Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007597

Physical Location & Seq

Genomic Coordinates Reverse (-)

2848108 .. 2851854

Gene Structure 3747 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1040600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGAAGTTTATGAAGCTGGGGTCCAAGCCTGATGCATTTCAGGCTGATGGCAAGTTTATCAGATATGTGTCGTCTGAACTGCAAACGGATGTCGTTATTAATGTTGGGGAAGTGAAGTTTTACCTTCACAAGTTCCCTCTCTTGTCCAAGAGCAATCGCCTGCAAAAACTAGTATCAAAAGCCAATGAGGATTCTGAAGAAATTGACATGGTTGATTTTCCTGGTGGGCCAAAATCCTTTGAAATTTGTGCAAAGTTTTGCTATGGAATGACTGTTACTCTCAACGCTTACAATGTTGTGGCTGCTCGATGTGCTGCTGAGTATCTGGAAATGACTGAGGGTGTTGATCACAGTAACCTAATATACAAAATTGAGGTGTTCCTTAACTCAAGCATCTTCCGTAGCTGGAAAGATTCCATTATTGTGCTACAAACCACTAAACCTCTTCTGTCGTGGTCTGAAGATCTGAAGATTGCTGGAAGATGCATAGACTCAATTGCTTCTAAAACATCTGTAGATCCTGCCAACATCACTTGGTCCTACACATATAACAGAAAATTAGCAGAGCCTGATAAAATTATTGAAGAAGGGATGAATTATCGAGACAAATTTGAAAAAGTTCCAAGGGATTGGTGGGTTGAAGACATATGTGAGCTGGATATTGATCTCTACAAACGAGTTATTATTGCTGTGAAATCGAAGGGAAGAATGGATGGCAGTGTTATTGGGGAGGCTCTGAAGACTTATGCAGTTAGATGGTTGCCAGATTCTGTTGATGCACTGGTTTCGGATGAACACGCCTTGAGGAACAAATCTCTGGTAGAAACAATTATTTGCTTATTGCCGTCTGACAAAGGTATGGGTTGTTCATCCAGTTTCTTGCTGAAACTTTTGAAAGTTTCTATTTTGGTTGGAGCAGACAAGTCGTTGAGGGAGGAGTTGGTGAAGAGGATCAGTTTGAAGTTGCATGAAGCTCGTGTAGGTGATTTGTTGATCCCCGCACGATCTCCTCAAATTACTATATATGACGTTGAGTTGGTTCAGTCTGTTGTGAGACAGTATGTGATGCATGAAAAGTATAAGTGGGATTTGGATGTTGTCGAGAAGAATGAAAAGGGGACTGATACTTTTATATTAGGGCATGGTTCGTTGTTGGGTGTTGGTAGACTGGTCGATGGGTATCTTGCGGAAATTGCACATGACCCAAATCTTAACCTCAGTAGTTTCGTAGAGTTGTCACAATCAGTTCCCGAGTCAGCCAGGCCAATTCACGATGGGTTGTACAAAGCCATTGACATCTACCTGAAGGTATGCGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005886 GO:0006810 GO:0007275 GO:0008104 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009909 GO:0009911 GO:0009914 GO:0009926 GO:0010154 GO:0010229 GO:0010540 GO:0010817 GO:0012505 GO:0016020 GO:0022414 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0033036 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0045176 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051094 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071944 GO:0090567 GO:0097708 GO:0099402 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB NPH3

Protein Analysis

440

Amino Acids

49.52

Weight (kDa)

5.87

Isoelectric Point (pI)

34.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	25 - 117	8.2e-08	BTB/POZ domain
NPH3	PF03000	210 - 436	7.8e-71	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007597)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31820 AT4G31820
fragaria_vesca	FvH4_2g41130 FvH4_2g41130 FvH4_2g41130 FvH4_2g41130
malus_domestica	MD08G1057100.v1.1 MD15G1040500.v1.1 MD15G1040600.v1.1
prunus_persica	Prupe.1G393800_v2.0.a1
pyrus_communis	pycom08g04650 pycom08g04660 pycom15g03780
rosa_chinensis	RchiOBHm_Chr5g0077711 RchiOBHm_Chr6g0299411
rosa_laevigata	RLG00000011434
rosa_multiflora	Rmu_co8314217.1_g000001
rosa_roxburghii	Rroxscaffold_7G00168710
rosa_rugosa	Rorug06G0288000
rosa_samantha	Rh6AG399800 Rh6BG408200 Rh6CG413900 Rh6DG400900
rosa_wichuraiana	Rw6G034990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1165
AciI	CCGC	2 cut(s)	999, 1187
AclWI	GGATC	3 cut(s)	512, 959, 988
AcsI	RAATTY	2 cut(s)	243, 608
AcuI	CTGAAG	4 cut(s)	216, 480, 488, 758
AfaI	GTAC	1 cut(s)	1283
AgsI	TTSAA	6 cut(s)	242, 584, 614, 641, 895, 961
AhlI	ACTAGT	1 cut(s)	169
AjnI	CCWGG	2 cut(s)	220, 1259
AluBI	AGCT	4 cut(s)	16, 405, 655, 974
AluI	AGCT	4 cut(s)	16, 405, 655, 974
Alw26I	GTCTC	2 cut(s)	597, 1048
AlwI	GGATC	3 cut(s)	512, 959, 988
AlwNI	CAGNNNCTG	2 cut(s)	569, 770
Ama87I	CYCGRG	1 cut(s)	1250
AoxI	GGCC	2 cut(s)	227, 1262
ApeKI	GCWGC	2 cut(s)	302, 314
ApoI	RAATTY	2 cut(s)	243, 608
AseI	ATTAAT	1 cut(s)	99
Asp700I	GAANNNNTTC	1 cut(s)	618
AspS9I	GGNCC	3 cut(s)	21, 227, 537
AsuHPI	GGTGA	2 cut(s)	955, 995
AvaI	CYCGRG	1 cut(s)	1250
AvaII	GGWCC	2 cut(s)	21, 537
BauI	CACGAG	1 cut(s)	975
BbsI	GAAGAC	2 cut(s)	648, 746
BbvI	GCAGC	2 cut(s)	289, 301
BccI	CCATC	5 cut(s)	41, 707, 750, 1169, 1268
BceAI	ACGGC	1 cut(s)	829
BcgI	CGANNNNNNTGC	6 cut(s)	768, 802, 906, 940, 956, 990
BciT130I	CCWGG	2 cut(s)	222, 1261
BclI	TGATCA	1 cut(s)	346
BcoDI	GTCTC	2 cut(s)	597, 1048
BcuI	ACTAGT	1 cut(s)	169
BfaI	CTAG	1 cut(s)	170
BfmI	CTRYAG	1 cut(s)	513
BglII	AGATCT	1 cut(s)	463
BisI	GCNGC	2 cut(s)	303, 315
BlsI	GCNGC	2 cut(s)	304, 316
Bme1390I	CCNGG	2 cut(s)	222, 1261
Bme18I	GGWCC	2 cut(s)	21, 537
BmeT110I	CYCGRG	1 cut(s)	1250
BmgT120I	GGNCC	3 cut(s)	21, 227, 537
BmiI	GGNNCC	1 cut(s)	22
BmrFI	CCNGG	2 cut(s)	222, 1261
BmsI	GCATC	5 cut(s)	22, 402, 473, 766, 1056
BpiI	GAAGAC	2 cut(s)	648, 746
BpuEI	CTTGAG	2 cut(s)	373, 823
BsaBI	GATNNNNATC	2 cut(s)	663, 1179
BsaJI	CCNNGG	1 cut(s)	623
Bse1I	ACTGG	3 cut(s)	786, 873, 1173
Bse8I	GATNNNNATC	2 cut(s)	663, 1179
BseBI	CCWGG	2 cut(s)	222, 1261
BseDI	CCNNGG	1 cut(s)	623
BseGI	GGATG	6 cut(s)	94, 597, 718, 796, 869, 1099
BseJI	GATNNNNATC	2 cut(s)	663, 1179
BseMII	CTCAG	3 cut(s)	309, 327, 1231
BseNI	ACTGG	3 cut(s)	786, 873, 1173
BseRI	GAGGAG	2 cut(s)	950, 999
BseXI	GCAGC	2 cut(s)	289, 301
BseYI	CCCAGC	1 cut(s)	16
BshFI	GGCC	2 cut(s)	229, 1264
BsiHKCI	CYCGRG	1 cut(s)	1250
BslFI	GGGAC	1 cut(s)	1132
BsmAI	GTCTC	2 cut(s)	597, 1048
BsmFI	GGGAC	1 cut(s)	1132
BsnI	GGCC	2 cut(s)	229, 1264
BsoBI	CYCGRG	1 cut(s)	1250
Bsp1407I	TGTACA	1 cut(s)	1281
Bsp143I	GATC	7 cut(s)	346, 463, 517, 664, 951, 993, 1004
BspACI	CCGC	2 cut(s)	999, 1187
BspANI	GGCC	2 cut(s)	229, 1264
BspCNI	CTCAG	3 cut(s)	310, 328, 1230
BspLI	GGNNCC	1 cut(s)	22
BspPI	GGATC	3 cut(s)	512, 959, 988
BsrGI	TGTACA	1 cut(s)	1281
BsrI	ACTGG	3 cut(s)	786, 873, 1173
BssECI	CCNNGG	1 cut(s)	623
BssMI	GATC	7 cut(s)	346, 463, 517, 664, 951, 993, 1004
BssSI	CACGAG	1 cut(s)	975
BssT1I	CCWWGG	1 cut(s)	623
Bst2BI	CACGAG	1 cut(s)	975
Bst2UI	CCWGG	2 cut(s)	222, 1261
Bst4CI	ACNGT	3 cut(s)	274, 353, 1059
Bst6I	CTCTTC	2 cut(s)	450, 941
BstAUI	TGTACA	1 cut(s)	1281
BstC8I	GCNNGC	1 cut(s)	161
BstDEI	CTNAG	3 cut(s)	318, 336, 1217
BstF5I	GGATG	6 cut(s)	94, 597, 718, 796, 869, 1099
BstKTI	GATC	7 cut(s)	349, 466, 520, 667, 954, 996, 1007
BstMAI	GTCTC	2 cut(s)	597, 1048
BstMBI	GATC	7 cut(s)	346, 463, 517, 664, 951, 993, 1004
BstMWI	GCNNNNNNNGC	3 cut(s)	41, 311, 1193
BstNI	CCWGG	2 cut(s)	222, 1261
BstSCI	CCNGG	2 cut(s)	220, 1259
BstSFI	CTRYAG	1 cut(s)	513
BstV1I	GCAGC	2 cut(s)	289, 301
BstV2I	GAAGAC	2 cut(s)	648, 746
BstX2I	RGATCY	2 cut(s)	463, 517
BstXI	CCANNNNNNTGG	1 cut(s)	630
BstYI	RGATCY	2 cut(s)	463, 517
BsuRI	GGCC	2 cut(s)	229, 1264
BtsCI	GGATG	6 cut(s)	94, 597, 718, 796, 869, 1099
BtsI	GCAGTG	1 cut(s)	724
BtsIMutI	CAGTG	2 cut(s)	724, 779
Cac8I	GCNNGC	1 cut(s)	161
CaiI	CAGNNNCTG	2 cut(s)	569, 770
Cfr13I	GGNCC	3 cut(s)	21, 227, 537
Csp6I	GTAC	1 cut(s)	1282
CviAII	CATG	5 cut(s)	208, 968, 1070, 1142, 1199
CviQI	GTAC	1 cut(s)	1282
DdeI	CTNAG	3 cut(s)	318, 336, 1217
DpnI	GATC	7 cut(s)	348, 465, 519, 666, 953, 995, 1006
DpnII	GATC	7 cut(s)	346, 463, 517, 664, 951, 993, 1004
Eam1104I	CTCTTC	2 cut(s)	450, 941
EarI	CTCTTC	2 cut(s)	450, 941
Eco130I	CCWWGG	1 cut(s)	623
Eco47I	GGWCC	2 cut(s)	21, 537
Eco57I	CTGAAG	4 cut(s)	216, 480, 488, 758
Eco88I	CYCGRG	1 cut(s)	1250
EcoRII	CCWGG	2 cut(s)	220, 1259
EcoT14I	CCWWGG	1 cut(s)	623
EcoT22I	ATGCAT	3 cut(s)	37, 488, 1071
ErhI	CCWWGG	1 cut(s)	623
FaeI	CATG	5 cut(s)	211, 971, 1073, 1145, 1202
FaqI	GGGAC	1 cut(s)	1132
FatI	CATG	5 cut(s)	207, 967, 1069, 1141, 1198
FauI	CCCGC	1 cut(s)	1006
FauNDI	CATATG	1 cut(s)	647
FbaI	TGATCA	1 cut(s)	346
FblI	GTMKAC	1 cut(s)	1165
Fnu4HI	GCNGC	2 cut(s)	303, 315
FokI	GGATG	6 cut(s)	101, 604, 725, 803, 856, 1106
Fsp4HI	GCNGC	2 cut(s)	303, 315
FspBI	CTAG	1 cut(s)	170
GluI	GCNGC	2 cut(s)	303, 315
GsaI	CCCAGC	1 cut(s)	20
HaeIII	GGCC	2 cut(s)	229, 1264
Hin1II	CATG	5 cut(s)	211, 971, 1073, 1145, 1202
HinfI	GANTC	5 cut(s)	191, 413, 491, 767, 1253
HphI	GGTGA	2 cut(s)	955, 995
Hpy166II	GTNNAC	1 cut(s)	1166
Hpy188I	TCNGA	8 cut(s)	62, 76, 196, 460, 468, 738, 790, 850
Hpy188III	TCNNGA	5 cut(s)	326, 602, 1102, 1250, 1271
Hpy8I	GTNNAC	1 cut(s)	1166
HpyAV	CCTTC	4 cut(s)	134, 581, 694, 1300
HpyCH4III	ACNGT	3 cut(s)	274, 353, 1059
HpyCH4IV	ACGT	1 cut(s)	1029
HpyF10VI	GCNNNNNNNGC	3 cut(s)	41, 311, 1193
HpyF3I	CTNAG	3 cut(s)	318, 336, 1217
HpySE526I	ACGT	1 cut(s)	1029
Hsp92II	CATG	5 cut(s)	211, 971, 1073, 1145, 1202
Ksp22I	TGATCA	1 cut(s)	346
Kzo9I	GATC	7 cut(s)	346, 463, 517, 664, 951, 993, 1004
LmnI	GCTCC	1 cut(s)	914
Lsp1109I	GCAGC	2 cut(s)	289, 301
LweI	GCATC	5 cut(s)	22, 402, 473, 766, 1056
MaeI	CTAG	1 cut(s)	170
MaeII	ACGT	1 cut(s)	1029
MaeIII	GTNAC	3 cut(s)	274, 353, 1236
MalI	GATC	7 cut(s)	348, 465, 519, 666, 953, 995, 1006
MboI	GATC	7 cut(s)	346, 463, 517, 664, 951, 993, 1004
MfeI	CAATTG	1 cut(s)	495
MflI	RGATCY	2 cut(s)	463, 517
MlyI	GAGTC	2 cut(s)	485, 1262
MmeI	TCCRAC	1 cut(s)	892
Mph1103I	ATGCAT	3 cut(s)	37, 488, 1071
MroXI	GAANNNNTTC	1 cut(s)	618
MseI	TTAA	4 cut(s)	99, 384, 1212, 1318
MslI	CAYNNNNRTG	1 cut(s)	422
MspR9I	CCNGG	2 cut(s)	222, 1261
MunI	CAATTG	1 cut(s)	495
MvaI	CCWGG	2 cut(s)	222, 1261
MwoI	GCNNNNNNNGC	3 cut(s)	41, 311, 1193
NdeI	CATATG	1 cut(s)	647
NdeII	GATC	7 cut(s)	346, 463, 517, 664, 951, 993, 1004
NlaIII	CATG	5 cut(s)	211, 971, 1073, 1145, 1202
NlaIV	GGNNCC	1 cut(s)	22
NmuCI	GTSAC	1 cut(s)	1236
NsiI	ATGCAT	3 cut(s)	37, 488, 1071
PdmI	GAANNNNTTC	1 cut(s)	618
PfeI	GAWTC	3 cut(s)	191, 413, 767
PkrI	GCNGC	2 cut(s)	304, 316
PleI	GAGTC	2 cut(s)	485, 1261
PpsI	GAGTC	2 cut(s)	485, 1261
PshBI	ATTAAT	1 cut(s)	99
Psp6I	CCWGG	2 cut(s)	220, 1259
PspFI	CCCAGC	1 cut(s)	16
PspGI	CCWGG	2 cut(s)	220, 1259
PspN4I	GGNNCC	1 cut(s)	22
PspPI	GGNCC	3 cut(s)	21, 227, 537
PsrI	GAACNNNNNNTAC	2 cut(s)	850, 882
PstNI	CAGNNNCTG	2 cut(s)	569, 770
PsuI	RGATCY	2 cut(s)	463, 517
RsaI	GTAC	1 cut(s)	1283
RsaNI	GTAC	1 cut(s)	1282
RseI	CAYNNNNRTG	1 cut(s)	422
SaqAI	TTAA	4 cut(s)	99, 384, 1212, 1318
SatI	GCNGC	2 cut(s)	303, 315
Sau3AI	GATC	7 cut(s)	346, 463, 517, 664, 951, 993, 1004
Sau96I	GGNCC	3 cut(s)	21, 227, 537
SchI	GAGTC	2 cut(s)	485, 1262
ScrFI	CCNGG	2 cut(s)	222, 1261
SfaNI	GCATC	5 cut(s)	22, 402, 473, 766, 1056
SfcI	CTRYAG	1 cut(s)	513
SinI	GGWCC	2 cut(s)	21, 537
SmiMI	CAYNNNNRTG	1 cut(s)	422
SmlI	CTYRAG	2 cut(s)	388, 802
SmoI	CTYRAG	2 cut(s)	388, 802
SpeI	ACTAGT	1 cut(s)	169
SsiI	CCGC	2 cut(s)	999, 1187
SspMI	CTAG	1 cut(s)	170
StyD4I	CCNGG	2 cut(s)	220, 1259
StyI	CCWWGG	1 cut(s)	623
TaaI	ACNGT	3 cut(s)	274, 353, 1059
TaiI	ACGT	1 cut(s)	1032
TaqI	TCGA	5 cut(s)	307, 601, 698, 1101, 1173
TatI	WGTACW	1 cut(s)	1281
TfiI	GAWTC	3 cut(s)	191, 413, 767
Tru1I	TTAA	4 cut(s)	99, 384, 1212, 1318
Tru9I	TTAA	4 cut(s)	99, 384, 1212, 1318
TscAI	CASTG	2 cut(s)	724, 786
TseFI	GTSAC	1 cut(s)	1236
TseI	GCWGC	2 cut(s)	302, 314
Tsp45I	GTSAC	1 cut(s)	1236
TspDTI	ATGAA	8 cut(s)	17, 26, 608, 807, 858, 984, 1086, 1125
TspGWI	ACGGA	2 cut(s)	101, 389
TspRI	CASTG	2 cut(s)	724, 786
VpaK11BI	GGWCC	2 cut(s)	21, 537
VspI	ATTAAT	1 cut(s)	99
XapI	RAATTY	2 cut(s)	243, 608
XcmI	CCANNNNNNNNNTGG	1 cut(s)	630
XmiI	GTMKAC	1 cut(s)	1165
XmnI	GAANNNNTTC	1 cut(s)	618
XspI	CTAG	1 cut(s)	170
Zsp2I	ATGCAT	3 cut(s)	37, 488, 1071

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.