MD15G1058200.v1.1

BAAT / Acyl-CoA thioester hydrolase C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009765
Physical Location & Seq
Forward (+)
3909906 .. 3910830
925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1058200.v1.1.491

Sequence Viewer

Length: 405 bp
ATGTTCTGTTTTATGGTTCCGTGTGTTCCTTTAAGTTTTCGAATTCTGTCGGCAGTTATTCGACAGCGCAGGGTTGTTGTAGAAAACAATCATGGGGAGAAGCTTGTCGGGATATTACATGAGACGGGTTCGAAGGAGCTCGTTGTGGTGTGCCATGGCATACACTCTTCAAAGGAACGCATTCCTATGGTGAACATTGCCGCTGTGTTAGAAAACGATGGTATAAGCGCTTTCCGCTTTGACTTCGCTGGAAATGGGGAAAGTGAAGGTTTTTTTCGATATGGTCACTATCGGAGAGAAGCTGATGATATACGCGCTGTAGTTCAATACTTTCGTGGGAAGGATTATGTAGTAACTGCAGTTGTTGGTCACAGTAAAGGTCTGTCTATAATTTTGCTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Hydrolase_4 Ribosomal_S10

Protein Analysis

135

Amino Acids

15.06

Weight (kDa)

8.52

Isoelectric Point (pI)

24.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 45 - 131 5.7e-08 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 315
AciI CCGC 2 cut(s) 201, 235
AcsI RAATTY 1 cut(s) 42
AfeI AGCGCT 1 cut(s) 229
AgsI TTSAA 2 cut(s) 171, 326
AluBI AGCT 3 cut(s) 103, 139, 302
AluI AGCT 3 cut(s) 103, 139, 302
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 116
Aor51HI AGCGCT 1 cut(s) 229
ApoI RAATTY 1 cut(s) 42
Asp700I GAANNNNTTC 1 cut(s) 180
AspLEI GCGC 3 cut(s) 69, 230, 317
AsuHPI GGTGA 1 cut(s) 202
AsuII TTCGAA 2 cut(s) 40, 131
BanII GRGCYC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 141
BccI CCATC 1 cut(s) 212
BcoDI GTCTC 1 cut(s) 116
BfmI CTRYAG 2 cut(s) 318, 357
BfoI RGCGCY 1 cut(s) 231
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 18
Bpu14I TTCGAA 2 cut(s) 40, 131
BsaJI CCNNGG 1 cut(s) 154
Bse3DI GCAATG 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 154
BseMI GCAATG 1 cut(s) 195
Bsh1236I CGCG 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 116
BsmBI CGTCTC 1 cut(s) 116
BsmI GAATGC 1 cut(s) 180
Bsp119I TTCGAA 2 cut(s) 40, 131
Bsp1286I GDGCHC 1 cut(s) 141
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 2 cut(s) 201, 235
BspFNI CGCG 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 18
BspMAI CTGCAG 1 cut(s) 361
BspT104I TTCGAA 2 cut(s) 40, 131
BsrDI GCAATG 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 154
Bst4CI ACNGT 1 cut(s) 374
Bst6I CTCTTC 1 cut(s) 172
BstBI TTCGAA 2 cut(s) 40, 131
BstDSI CCRYGG 1 cut(s) 154
BstFNI CGCG 1 cut(s) 315
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 3 cut(s) 69, 230, 317
BstMAI GTCTC 1 cut(s) 116
BstMWI GCNNNNNNNGC 1 cut(s) 234
BstSFI CTRYAG 2 cut(s) 318, 357
BstUI CGCG 1 cut(s) 315
BtgI CCRYGG 1 cut(s) 154
CfoI GCGC 3 cut(s) 69, 230, 317
CviAII CATG 3 cut(s) 92, 119, 155
CviJI RGCY 3 cut(s) 103, 139, 302
CviKI_1 RGCY 3 cut(s) 103, 139, 302
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 141
Eco47III AGCGCT 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 154
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 3 cut(s) 95, 122, 158
FatI CATG 3 cut(s) 91, 118, 154
Fnu4HI GCNGC 1 cut(s) 201
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 1 cut(s) 201
GlaI GCGC 3 cut(s) 68, 229, 316
GluI GCNGC 1 cut(s) 201
HaeII RGCGCY 1 cut(s) 231
HhaI GCGC 3 cut(s) 69, 230, 317
Hin1II CATG 3 cut(s) 95, 122, 158
Hin6I GCGC 3 cut(s) 67, 228, 315
HinP1I GCGC 3 cut(s) 67, 228, 315
HindIII AAGCTT 1 cut(s) 101
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 1 cut(s) 294
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 3 cut(s) 127, 260, 334
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 234
Hsp92II CATG 3 cut(s) 95, 122, 158
HspAI GCGC 3 cut(s) 67, 228, 315
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 2 cut(s) 55, 234
MaeIII GTNAC 3 cut(s) 284, 352, 368
MboII GAAGA 1 cut(s) 159
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 2 cut(s) 42, 390
MroXI GAANNNNTTC 1 cut(s) 180
MseI TTAA 1 cut(s) 32
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 203
Mva1269I GAATGC 1 cut(s) 180
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 234
NcoI CCATGG 1 cut(s) 154
NlaIII CATG 3 cut(s) 95, 122, 158
NlaIV GGNNCC 1 cut(s) 18
NmuCI GTSAC 2 cut(s) 284, 368
NspV TTCGAA 2 cut(s) 40, 131
PctI GAATGC 1 cut(s) 180
PdmI GAANNNNTTC 1 cut(s) 180
PkrI GCNGC 1 cut(s) 202
Psp124BI GAGCTC 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 18
PstI CTGCAG 1 cut(s) 361
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 1 cut(s) 141
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 1 cut(s) 201
SduI GDGCHC 1 cut(s) 141
SetI ASST 5 cut(s) 105, 141, 271, 304, 382
SfcI CTRYAG 2 cut(s) 318, 357
SfuI TTCGAA 2 cut(s) 40, 131
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 2 cut(s) 42, 390
SsiI CCGC 2 cut(s) 201, 235
SstI GAGCTC 1 cut(s) 141
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 1 cut(s) 374
TaqI TCGA 4 cut(s) 40, 61, 131, 277
TasI AATT 2 cut(s) 42, 390
TauI GCSGC 1 cut(s) 203
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TseFI GTSAC 2 cut(s) 284, 368
Tsp45I GTSAC 2 cut(s) 284, 368
TspGWI ACGGA 1 cut(s) 9
XapI RAATTY 1 cut(s) 42
XmnI GAANNNNTTC 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.