Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001642

Physical Location & Seq

Genomic Coordinates Forward (+)

4123644 .. 4129713

Gene Structure 6070 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1061300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGAACGGCAGCGGAGAGGGCCTCGCGGCTCCGGCGACGTCAGGGGCGGCCGTATCGCCGCTCGACTGGAAGTTTTCGCAGGTCTTCGGAGAAAGGACAGCCGGCGAAGAAGTTCAGGAAGTTGATATAATATCTGCTATTGAATTCAATAGAACTGGGGACCACCTTGCCACTGGAGATCGTGGAGGCCGGGTGGTATTGTTTGAAAGAACGGATATAAAGGATCATGGTGGACATCGGAGAGATCTGGAGAGGATGGATTATTCAATCAGTAGGCATCCTGAGTTCCGCTATAAAACCGAATTTCAAAGTCATGAACCTGAGTTTGATTATCTTAAGAGTTTGGAAATTGAGGAAAAAATCAACAAGATCAGATGGTGTCAAACAGCTAATGGTGCACTGTTTCTTCTCTCCACTAATGACAAAACCATCAAATACTGGAAGGTCCAAGAGAAGAAGGTAAAGAAAGTATGTGAGATGAATGTGGATGCTTCTAAATATATGGAAAATGGTTCAGTTGCTAGTTCTATGTCATCAAGCTCAAAACCGTATCTTGCAAATGAAGCATGTAAAGAGAGCCCAGTCAGTTCTCCAAGCAATGACTCTTCATTTCCATCTCTGCACTTGCCAGTGGTGACCAGTCAAGAGACGAGCCTTGTGGCTAGATGTCGAAGAGTGTATACCCATGCCCATGACTATCATATTAATTCGATTTCAAATAACAGCGATGGTGAAACTTTTATATCAGCTGATGACCTGCGAATTAATCTTTGGAACCTAGAGATTAGCAATCAAAGTTTTAATATTGTTGACGTGAAGCCTGCAAACATGGAGGATCTAACTGAGGTGATAACATGCGCAGAATTTCACCCTACTCATTGTAACATGTTGGCGTACAGTAGCTCCAAAGGCTCAATTCGTCTTATCGATCTGCGGCAGTCAGCTTTATGTGACACTCATAGCAAATTGTTTGAAGAACAAGAAGCGCCCGGTTCAAGATCTTTTTTCACTGAAATAATAGCATCGATTTCAGATATTAAGTTTGCTAAGAATGGAAGACATATACTTAGTCGGGATTACATGACTCTTAAGTTATGGGACATCAACATGGATTCTGGCCCAGTTGCAACTTTCCAGGTTCATGAATATCTAAGACCAAAGCTTTGTGATTTATACGAAAATGATTCCATCTTCGATAAGTTTGAGTGCTGTCTAAGTGGAGATGGACACCGAGTGGCAACAGGTTCTTACAGCAATCTGTTTCGGGTGTTTGGTTGTTCTGAAGGGAGCACAGAAGCAACAACATTGGAGGCCAGCAAAAACCCCATGAGGAGACAAGTTCAAACGCCAGCAAGGCCTTCTAGATCTTTGGGCAGCCTTCCTCGTGTTCTCAGGCGAGGTGTGGATAACTCTGGAGCTGACGCAAACGGAAATTCTTTTGATTTCACAACAAAGTTGCTACATCTTGCATGGCACCCTTCTGAGAATTCAATCGCCTGTGCTGCTTCAAACAGCCTTTACATGTACTATGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000159 GO:0000278 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0008287 GO:0009987 GO:0010921 GO:0016311 GO:0019208 GO:0019220 GO:0019222 GO:0019538 GO:0019888 GO:0030234 GO:0031323 GO:0031399 GO:0032268 GO:0032502 GO:0032991 GO:0035303 GO:0035304 GO:0036211 GO:0043170 GO:0043412 GO:0043666 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051174 GO:0051246 GO:0051336 GO:0060255 GO:0065007 GO:0065009 GO:0070262 GO:0071704 GO:0080090 GO:0098772 GO:1901564 GO:1902494 GO:1903293

KEGG Pathways

Metabolic & Signaling

ko03015 ko04071 ko04111 ko04151 ko04152 ko04261 ko04390 ko04391 ko04530 ko04728 ko05142 ko05160 ko05165 map03015 map04071 map04111 map04151 map04152 map04261 map04390 map04391 map04530 map04728 map05142 map05160 map05165

Pfam Domains

Protein Families

WD40

Protein Analysis

512

Amino Acids

57.21

Weight (kDa)

5.82

Isoelectric Point (pI)

52.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_Prp19	PF24814	225 - 369	2.1e-06	Prp19 WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001642)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17720 AT1G17720 AT1G51690 AT1G51690 AT1G51690 AT1G51690 AT1G51690
fragaria_vesca	FvH4_2g32090 FvH4_2g32090 FvH4_2g32090 FvH4_2g32090 FvH4_2g32090 FvH4_2g32090 FvH4_6g33020 FvH4_6g33020 FvH4_6g33020 FvH4_6g33020
malus_domestica	MD08G1043800.v1.1 MD08G1043900.v1.1 MD08G1044000.v1.1 MD09G1195400.v1.1 MD15G1061300.v1.1 MD17G1176000.v1.1
prunus_persica	Prupe.1G414000_v2.0.a1 Prupe.1G414000_v2.0.a1 Prupe.3G052100_v2.0.a1 Prupe.3G052100_v2.0.a1
pyrus_communis	pycom08g03540 pycom15g05740 pycom17g18330
rosa_chinensis	RchiOBHm_Chr2g0142561 RchiOBHm_Chr6g0310651
rosa_laevigata	RLG00000010469 RLG00000019967
rosa_multiflora	Rmu_co8494963.1_g000001 Rmu_sc0000482.1_g000004 Rmu_sc0007390.1_g000003
rosa_roxburghii	Rroxscaffold_2G00102520 Rroxscaffold_7G00158380
rosa_rugosa	Rorug02G0373900 Rorug02G0374000 Rorug06G0381900 Rorug06G0381900 Rorug06G0381900
rosa_samantha	Rh2BG435300 Rh2DG445300 Rh6BG505400
rosa_wichuraiana	Rw2G034890 Rw6G043120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	41
Acc16I	TGCGCA	1 cut(s)	859
Acc36I	ACCTGC	2 cut(s)	70, 765
AccB1I	GGYRCC	1 cut(s)	1473
AccBSI	CCGCTC	1 cut(s)	61
AccI	GTMKAC	1 cut(s)	680
AccII	CGCG	1 cut(s)	26
AciI	CCGC	6 cut(s)	12, 26, 47, 59, 289, 934
AclWI	GGATC	2 cut(s)	231, 843
AcoI	YGGCCR	1 cut(s)	48
AcsI	RAATTY	5 cut(s)	143, 302, 863, 1432, 1486
AcuI	CTGAAG	1 cut(s)	1302
AcyI	GRCGYC	1 cut(s)	38
AdeI	CACNNNGTG	1 cut(s)	1234
AfaI	GTAC	2 cut(s)	896, 1526
AflII	CTTAAG	2 cut(s)	335, 1088
AflIII	ACRYGT	2 cut(s)	885, 1521
AjiI	CACGTC	1 cut(s)	814
AjnI	CCWGG	1 cut(s)	1134
AjuI	GAANNNNNNNTTGG	2 cut(s)	754, 786
AluBI	AGCT	7 cut(s)	389, 540, 749, 903, 944, 1162, 1418
AluI	AGCT	7 cut(s)	389, 540, 749, 903, 944, 1162, 1418
Alw21I	GWGCWC	2 cut(s)	400, 1292
Alw26I	GTCTC	2 cut(s)	641, 1327
Alw44I	GTGCAC	1 cut(s)	396
AlwI	GGATC	2 cut(s)	231, 843
AoxI	GGCC	6 cut(s)	19, 48, 187, 1117, 1311, 1355
ApaLI	GTGCAC	1 cut(s)	396
ApeKI	GCWGC	3 cut(s)	9, 1374, 1502
ApoI	RAATTY	5 cut(s)	143, 302, 863, 1432, 1486
ArsI	GACNNNNNNTTYG	2 cut(s)	1146, 1178
AseI	ATTAAT	2 cut(s)	705, 765
Asp700I	GAANNNNTTC	1 cut(s)	111
AspLEI	GCGC	2 cut(s)	860, 988
AspS9I	GGNCC	4 cut(s)	19, 160, 445, 1118
AsuC2I	CCSGG	2 cut(s)	191, 990
AsuHPI	GGTGA	4 cut(s)	646, 743, 859, 860
AvaII	GGWCC	2 cut(s)	160, 445
BaeGI	GKGCMC	1 cut(s)	400
BanI	GGYRCC	1 cut(s)	1473
BanII	GRGCYC	1 cut(s)	581
BarI	GAAGNNNNNNTAC	2 cut(s)	664, 696
BauI	CACGAG	1 cut(s)	1383
BbsI	GAAGAC	2 cut(s)	76, 1063
Bbv12I	GWGCWC	2 cut(s)	400, 1292
BbvI	GCAGC	3 cut(s)	21, 1386, 1489
BccI	CCATC	7 cut(s)	250, 369, 437, 622, 722, 1196, 1217
BceAI	ACGGC	2 cut(s)	22, 35
BciT130I	CCWGG	1 cut(s)	1136
BcnI	CCSGG	2 cut(s)	191, 990
BcoDI	GTCTC	2 cut(s)	641, 1327
BfaI	CTAG	4 cut(s)	522, 663, 779, 1362
BfoI	RGCGCY	1 cut(s)	989
BfrI	CTTAAG	2 cut(s)	335, 1088
BfuAI	ACCTGC	2 cut(s)	70, 765
BglI	GCCNNNNNGGC	1 cut(s)	1354
BglII	AGATCT	3 cut(s)	244, 998, 1364
BisI	GCNGC	7 cut(s)	10, 27, 48, 59, 935, 1375, 1503
BlsI	GCNGC	7 cut(s)	11, 28, 49, 60, 936, 1376, 1504
Bme1390I	CCNGG	3 cut(s)	191, 990, 1136
Bme18I	GGWCC	2 cut(s)	160, 445
BmgBI	CACGTC	1 cut(s)	814
BmgT120I	GGNCC	4 cut(s)	19, 160, 445, 1118
BmiI	GGNNCC	4 cut(s)	30, 161, 776, 1475
BmrFI	CCNGG	3 cut(s)	191, 990, 1136
BmrI	ACTGGG	3 cut(s)	165, 575, 1115
BmsI	GCATC	3 cut(s)	286, 478, 1031
BmuI	ACTGGG	3 cut(s)	165, 575, 1115
BpiI	GAAGAC	2 cut(s)	76, 1063
BplI	GAGNNNNNCTC	1 cut(s)	38
BpmI	CTGGAG	3 cut(s)	195, 269, 1434
BpuMI	CCSGG	2 cut(s)	191, 990
Bsa29I	ATCGAT	2 cut(s)	927, 1025
BsaHI	GRCGYC	1 cut(s)	38
BsaXI	ACNNNNNCTCC	4 cut(s)	177, 207, 887, 917
Bse118I	RCCGGY	1 cut(s)	101
Bse1I	ACTGG	8 cut(s)	71, 160, 178, 443, 581, 629, 639, 1121
Bse3DI	GCAATG	1 cut(s)	604
BseBI	CCWGG	1 cut(s)	1136
BseCI	ATCGAT	2 cut(s)	927, 1025
BseGI	GGATG	3 cut(s)	261, 277, 493
BseMI	GCAATG	1 cut(s)	604
BseMII	CTCAG	5 cut(s)	273, 312, 834, 1405, 1473
BseNI	ACTGG	8 cut(s)	71, 160, 178, 443, 581, 629, 639, 1121
BseRI	GAGGAG	1 cut(s)	1345
BseSI	GKGCMC	1 cut(s)	400
BseX3I	CGGCCG	1 cut(s)	48
BseXI	GCAGC	3 cut(s)	21, 1386, 1489
BsgI	GTGCAG	1 cut(s)	605
Bsh1236I	CGCG	1 cut(s)	26
Bsh1285I	CGRYCG	1 cut(s)	51
BshFI	GGCC	6 cut(s)	21, 50, 189, 1119, 1313, 1357
BshNI	GGYRCC	1 cut(s)	1473
BshVI	ATCGAT	2 cut(s)	927, 1025
BsiEI	CGRYCG	1 cut(s)	51
BsiHKAI	GWGCWC	2 cut(s)	400, 1292
BsiSI	CCGG	4 cut(s)	32, 102, 190, 990
BslFI	GGGAC	2 cut(s)	173, 1112
BsmAI	GTCTC	2 cut(s)	641, 1327
BsmBI	CGTCTC	1 cut(s)	641
BsmFI	GGGAC	2 cut(s)	173, 1112
BsnI	GGCC	6 cut(s)	21, 50, 189, 1119, 1313, 1357
Bsp1286I	GDGCHC	3 cut(s)	400, 581, 1292
Bsp143I	GATC	8 cut(s)	178, 223, 244, 369, 835, 928, 998, 1364
BspACI	CCGC	6 cut(s)	12, 26, 47, 59, 289, 934
BspANI	GGCC	6 cut(s)	21, 50, 189, 1119, 1313, 1357
BspCNI	CTCAG	5 cut(s)	274, 313, 835, 1404, 1474
BspDI	ATCGAT	2 cut(s)	927, 1025
BspFNI	CGCG	1 cut(s)	26
BspHI	TCATGA	2 cut(s)	313, 1141
BspLI	GGNNCC	4 cut(s)	30, 161, 776, 1475
BspMI	ACCTGC	2 cut(s)	70, 765
BspPI	GGATC	2 cut(s)	231, 843
BspT107I	GGYRCC	1 cut(s)	1473
BspTI	CTTAAG	2 cut(s)	335, 1088
BsrBI	CCGCTC	1 cut(s)	61
BsrDI	GCAATG	1 cut(s)	604
BsrFI	RCCGGY	1 cut(s)	101
BsrI	ACTGG	8 cut(s)	71, 160, 178, 443, 581, 629, 639, 1121
BssAI	RCCGGY	1 cut(s)	101
BssMI	GATC	8 cut(s)	178, 223, 244, 369, 835, 928, 998, 1364
BssNAI	GTATAC	1 cut(s)	681
BssNI	GRCGYC	1 cut(s)	38
BssSI	CACGAG	1 cut(s)	1383
Bst1107I	GTATAC	1 cut(s)	681
Bst2BI	CACGAG	1 cut(s)	1383
Bst2UI	CCWGG	1 cut(s)	1136
Bst4CI	ACNGT	3 cut(s)	402, 549, 899
Bst6I	CTCTTC	2 cut(s)	610, 667
BstACI	GRCGYC	1 cut(s)	38
BstAFI	CTTAAG	2 cut(s)	335, 1088
BstC8I	GCNNGC	4 cut(s)	103, 822, 1315, 1350
BstDEI	CTNAG	9 cut(s)	282, 321, 843, 1047, 1067, 1151, 1214, 1391, 1482
BstEII	GGTNACC	1 cut(s)	634
BstF5I	GGATG	3 cut(s)	261, 277, 493
BstFNI	CGCG	1 cut(s)	26
BstH2I	RGCGCY	1 cut(s)	989
BstHHI	GCGC	2 cut(s)	860, 988
BstKTI	GATC	8 cut(s)	181, 226, 247, 372, 838, 931, 1001, 1367
BstMAI	GTCTC	2 cut(s)	641, 1327
BstMBI	GATC	8 cut(s)	178, 223, 244, 369, 835, 928, 998, 1364
BstMCI	CGRYCG	1 cut(s)	51
BstMWI	GCNNNNNNNGC	7 cut(s)	18, 32, 395, 563, 909, 1354, 1502
BstNI	CCWGG	1 cut(s)	1136
BstNSI	RCATGY	4 cut(s)	570, 858, 889, 1525
BstPI	GGTNACC	1 cut(s)	634
BstSCI	CCNGG	3 cut(s)	189, 988, 1134
BstSLI	GKGCMC	1 cut(s)	400
BstUI	CGCG	1 cut(s)	26
BstV1I	GCAGC	3 cut(s)	21, 1386, 1489
BstV2I	GAAGAC	2 cut(s)	76, 1063
BstX2I	RGATCY	4 cut(s)	244, 835, 998, 1364
BstYI	RGATCY	4 cut(s)	244, 835, 998, 1364
BstZ17I	GTATAC	1 cut(s)	681
BstZI	CGGCCG	1 cut(s)	48
Bsu15I	ATCGAT	2 cut(s)	927, 1025
BsuRI	GGCC	6 cut(s)	21, 50, 189, 1119, 1313, 1357
BsuTUI	ATCGAT	2 cut(s)	927, 1025
BtgZI	GCGATG	1 cut(s)	741
BtrI	CACGTC	1 cut(s)	814
BtsCI	GGATG	3 cut(s)	261, 277, 493
BtsIMutI	CAGTG	4 cut(s)	171, 398, 636, 1008
BveI	ACCTGC	2 cut(s)	70, 765
Cac8I	GCNNGC	4 cut(s)	103, 822, 1315, 1350
CciI	TCATGA	2 cut(s)	313, 1141
CfoI	GCGC	2 cut(s)	860, 988
Cfr10I	RCCGGY	1 cut(s)	101
Cfr13I	GGNCC	4 cut(s)	19, 160, 445, 1118
ClaI	ATCGAT	2 cut(s)	927, 1025
CseI	GACGC	1 cut(s)	1430
Csp6I	GTAC	2 cut(s)	895, 1525
CviQI	GTAC	2 cut(s)	895, 1525
DdeI	CTNAG	9 cut(s)	282, 321, 843, 1047, 1067, 1151, 1214, 1391, 1482
DpnI	GATC	8 cut(s)	180, 225, 246, 371, 837, 930, 1000, 1366
DpnII	GATC	8 cut(s)	178, 223, 244, 369, 835, 928, 998, 1364
DraIII	CACNNNGTG	1 cut(s)	1234
EaeI	YGGCCR	1 cut(s)	48
EagI	CGGCCG	1 cut(s)	48
Eam1104I	CTCTTC	2 cut(s)	610, 667
EarI	CTCTTC	2 cut(s)	610, 667
EclXI	CGGCCG	1 cut(s)	48
Eco147I	AGGCCT	1 cut(s)	1357
Eco24I	GRGCYC	1 cut(s)	581
Eco47I	GGWCC	2 cut(s)	160, 445
Eco52I	CGGCCG	1 cut(s)	48
Eco57I	CTGAAG	1 cut(s)	1302
Eco91I	GGTNACC	1 cut(s)	634
EcoO109I	RGGNCCY	1 cut(s)	19
EcoO65I	GGTNACC	1 cut(s)	634
EcoRI	GAATTC	2 cut(s)	143, 1486
EcoRII	CCWGG	1 cut(s)	1134
EcoT22I	ATGCAT	1 cut(s)	1534
EcoT38I	GRGCYC	1 cut(s)	581
Esp3I	CGTCTC	1 cut(s)	641
FaqI	GGGAC	2 cut(s)	173, 1112
FblI	GTMKAC	1 cut(s)	680
Fnu4HI	GCNGC	7 cut(s)	10, 27, 48, 59, 935, 1375, 1503
FokI	GGATG	3 cut(s)	264, 268, 500
FriOI	GRGCYC	1 cut(s)	581
Fsp4HI	GCNGC	7 cut(s)	10, 27, 48, 59, 935, 1375, 1503
FspBI	CTAG	4 cut(s)	522, 663, 779, 1362
FspI	TGCGCA	1 cut(s)	859
GlaI	GCGC	2 cut(s)	859, 987
GluI	GCNGC	7 cut(s)	10, 27, 48, 59, 935, 1375, 1503
GsuI	CTGGAG	3 cut(s)	195, 269, 1434
HaeII	RGCGCY	1 cut(s)	989
HaeIII	GGCC	6 cut(s)	21, 50, 189, 1119, 1313, 1357
HapII	CCGG	4 cut(s)	32, 102, 190, 990
HgaI	GACGC	1 cut(s)	1430
HhaI	GCGC	2 cut(s)	860, 988
Hin1I	GRCGYC	1 cut(s)	38
Hin6I	GCGC	2 cut(s)	858, 986
HinP1I	GCGC	2 cut(s)	858, 986
HincII	GTYRAC	1 cut(s)	811
HindII	GTYRAC	1 cut(s)	811
HindIII	AAGCTT	1 cut(s)	1160
HinfI	GANTC	4 cut(s)	602, 1084, 1112, 1184
HpaII	CCGG	4 cut(s)	32, 102, 190, 990
HphI	GGTGA	4 cut(s)	646, 743, 859, 860
Hpy166II	GTNNAC	4 cut(s)	233, 398, 681, 811
Hpy188I	TCNGA	6 cut(s)	89, 240, 374, 1033, 1282, 1483
Hpy8I	GTNNAC	4 cut(s)	233, 398, 681, 811
Hpy99I	CGWCG	1 cut(s)	40
HpyAV	CCTTC	6 cut(s)	436, 451, 1277, 1368, 1388, 1488
HpyCH4III	ACNGT	3 cut(s)	402, 549, 899
HpyCH4IV	ACGT	2 cut(s)	38, 813
HpyCH4V	TGCA	7 cut(s)	398, 557, 622, 824, 1127, 1469, 1532
HpyF10VI	GCNNNNNNNGC	7 cut(s)	18, 32, 395, 563, 909, 1354, 1502
HpyF3I	CTNAG	9 cut(s)	282, 321, 843, 1047, 1067, 1151, 1214, 1391, 1482
HpySE526I	ACGT	2 cut(s)	38, 813
Hsp92I	GRCGYC	1 cut(s)	38
HspAI	GCGC	2 cut(s)	858, 986
KroI	GCCGGC	1 cut(s)	101
KroNI	GCCGGC	1 cut(s)	103
Kzo9I	GATC	8 cut(s)	178, 223, 244, 369, 835, 928, 998, 1364
LmnI	GCTCC	4 cut(s)	34, 908, 1287, 1415
Lsp1109I	GCAGC	3 cut(s)	21, 1386, 1489
LweI	GCATC	3 cut(s)	286, 478, 1031
MaeI	CTAG	4 cut(s)	522, 663, 779, 1362
MaeII	ACGT	2 cut(s)	38, 813
MaeIII	GTNAC	3 cut(s)	634, 881, 950
MalI	GATC	8 cut(s)	180, 225, 246, 371, 837, 930, 1000, 1366
MbiI	CCGCTC	1 cut(s)	61
MboI	GATC	8 cut(s)	178, 223, 244, 369, 835, 928, 998, 1364
MboII	GAAGA	9 cut(s)	76, 119, 398, 466, 597, 684, 986, 1068, 1183
MflI	RGATCY	4 cut(s)	244, 835, 998, 1364
MhlI	GDGCHC	3 cut(s)	400, 581, 1292
MlyI	GAGTC	2 cut(s)	596, 1078
Mph1103I	ATGCAT	1 cut(s)	1534
MroNI	GCCGGC	1 cut(s)	101
MroXI	GAANNNNTTC	1 cut(s)	111
MseI	TTAA	6 cut(s)	336, 705, 765, 801, 1038, 1089
MslI	CAYNNNNRTG	2 cut(s)	690, 1106
MspA1I	CMGCKG	2 cut(s)	12, 749
MspCI	CTTAAG	2 cut(s)	335, 1088
MspI	CCGG	4 cut(s)	32, 102, 190, 990
MspR9I	CCNGG	3 cut(s)	191, 990, 1136
MvaI	CCWGG	1 cut(s)	1136
MvnI	CGCG	1 cut(s)	26
MwoI	GCNNNNNNNGC	7 cut(s)	18, 32, 395, 563, 909, 1354, 1502
NaeI	GCCGGC	1 cut(s)	103
NciI	CCSGG	2 cut(s)	191, 990
NdeII	GATC	8 cut(s)	178, 223, 244, 369, 835, 928, 998, 1364
NgoMIV	GCCGGC	1 cut(s)	101
NlaIV	GGNNCC	4 cut(s)	30, 161, 776, 1475
NmuCI	GTSAC	2 cut(s)	634, 950
NsbI	TGCGCA	1 cut(s)	859
NsiI	ATGCAT	1 cut(s)	1534
NspI	RCATGY	4 cut(s)	570, 858, 889, 1525
PagI	TCATGA	2 cut(s)	313, 1141
PceI	AGGCCT	1 cut(s)	1357
PciI	ACATGT	2 cut(s)	885, 1521
PdiI	GCCGGC	1 cut(s)	103
PdmI	GAANNNNTTC	1 cut(s)	111
PfeI	GAWTC	2 cut(s)	1112, 1184
PkrI	GCNGC	7 cut(s)	11, 28, 49, 60, 936, 1376, 1504
PleI	GAGTC	2 cut(s)	596, 1078
PpsI	GAGTC	2 cut(s)	596, 1078
PscI	ACATGT	2 cut(s)	885, 1521
PshBI	ATTAAT	2 cut(s)	705, 765
Psp6I	CCWGG	1 cut(s)	1134
PspEI	GGTNACC	1 cut(s)	634
PspGI	CCWGG	1 cut(s)	1134
PspN4I	GGNNCC	4 cut(s)	30, 161, 776, 1475
PspPI	GGNCC	4 cut(s)	19, 160, 445, 1118
PsuI	RGATCY	4 cut(s)	244, 835, 998, 1364
PvuII	CAGCTG	1 cut(s)	749
RsaI	GTAC	2 cut(s)	896, 1526
RsaNI	GTAC	2 cut(s)	895, 1525
RseI	CAYNNNNRTG	2 cut(s)	690, 1106
SaqAI	TTAA	6 cut(s)	336, 705, 765, 801, 1038, 1089
SatI	GCNGC	7 cut(s)	10, 27, 48, 59, 935, 1375, 1503
Sau3AI	GATC	8 cut(s)	178, 223, 244, 369, 835, 928, 998, 1364
Sau96I	GGNCC	4 cut(s)	19, 160, 445, 1118
SchI	GAGTC	2 cut(s)	596, 1078
ScrFI	CCNGG	3 cut(s)	191, 990, 1136
SduI	GDGCHC	3 cut(s)	400, 581, 1292
SfaNI	GCATC	3 cut(s)	286, 478, 1031
SinI	GGWCC	2 cut(s)	160, 445
SmiMI	CAYNNNNRTG	2 cut(s)	690, 1106
SmlI	CTYRAG	2 cut(s)	335, 1088
SmoI	CTYRAG	2 cut(s)	335, 1088
SseBI	AGGCCT	1 cut(s)	1357
SsiI	CCGC	6 cut(s)	12, 26, 47, 59, 289, 934
SspI	AATATT	1 cut(s)	805
SspMI	CTAG	4 cut(s)	522, 663, 779, 1362
StuI	AGGCCT	1 cut(s)	1357
StyD4I	CCNGG	3 cut(s)	189, 988, 1134
TaaI	ACNGT	3 cut(s)	402, 549, 899
TaiI	ACGT	2 cut(s)	41, 816
TaqI	TCGA	6 cut(s)	63, 670, 710, 927, 1025, 1194
TatI	WGTACW	1 cut(s)	1524
TauI	GCSGC	4 cut(s)	29, 50, 61, 937
TfiI	GAWTC	2 cut(s)	1112, 1184
Tru1I	TTAA	6 cut(s)	336, 705, 765, 801, 1038, 1089
Tru9I	TTAA	6 cut(s)	336, 705, 765, 801, 1038, 1089
TscAI	CASTG	4 cut(s)	178, 405, 636, 1015
TseFI	GTSAC	2 cut(s)	634, 950
TseI	GCWGC	3 cut(s)	9, 1374, 1502
Tsp45I	GTSAC	2 cut(s)	634, 950
TspDTI	ATGAA	7 cut(s)	17, 330, 494, 576, 597, 1130, 1158
TspGWI	ACGGA	2 cut(s)	227, 1443
TspRI	CASTG	4 cut(s)	178, 405, 636, 1015
Vha464I	CTTAAG	2 cut(s)	335, 1088
VneI	GTGCAC	1 cut(s)	396
VpaK11BI	GGWCC	2 cut(s)	160, 445
VspI	ATTAAT	2 cut(s)	705, 765
XapI	RAATTY	5 cut(s)	143, 302, 863, 1432, 1486
XbaI	TCTAGA	1 cut(s)	1361
XceI	RCATGY	4 cut(s)	570, 858, 889, 1525
XcmI	CCANNNNNNNNNTGG	1 cut(s)	170
XmiI	GTMKAC	1 cut(s)	680
XmnI	GAANNNNTTC	1 cut(s)	111
XspI	CTAG	4 cut(s)	522, 663, 779, 1362
ZraI	GACGTC	1 cut(s)	39
Zsp2I	ATGCAT	1 cut(s)	1534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.