Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005015

Physical Location & Seq

Genomic Coordinates Forward (+)

10592132 .. 10595534

Gene Structure 3403 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1143500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 684 bp

ATGAGGAAGAGAGAGGAAGAAGATGCCCACCAGAACCCTCCCGCCGTCGACAAGCTTCAGCAAGTCGTGCACAGCAACTCTACAGGCGGTTGGGACAAGTGCTGGGAGCAAGGTTTGACCCCTTGGGATTTAGGGCAGCCAACGCCTGTTATTTCCCATCTTCATCGGACGGGAGCCCTTCCCAAGGGCAGGGCTCTTGTCCCTGGATGTGGCACTGGATATGATGTTGTAGAAATTGCATGTCCTGAACGTCATGCCACTGGATTGGACATTTCAGAGAATGCCATTAACAAAGCTCTGGAGTTGTTTTCCTCACTTCCCAAAGCACGGTACTTTACCTTCTTAAAGGTGGACTTTTTCACTTGGCATCCAACTGAATTGTTTGATCTCGTTTTTGATTATACGTTCTTCTGTGCTGTTGAACCAGACATGAGATCTGCATGGGCACGGAAGATCGCAGATATCTTAAAACCGGATGGGGAGCTCATAACACTGATGTTTCCTACCAGTGATCATATTGGTGGACCGCCTTACAAAGTGTCAGTTTCAGATTATGAAGAGGTTTTGCATCCCCTGGGCTTTAAGGCAACCTCCATCGTAGACAATGAACTAGCTGTCCGACCTCGCAAGGGAAGAGAGAAACTAGGAAGGTGGAAGAGGTCTCTAAGCAAATCCTCTCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006082 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008757 GO:0009056 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0016020 GO:0016143 GO:0016145 GO:0016740 GO:0016741 GO:0018708 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0031967 GO:0031975 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044248 GO:0044273 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0071704 GO:0071944 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPMT

Protein Analysis

228

Amino Acids

25.55

Weight (kDa)

6.45

Isoelectric Point (pI)

39.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPMT	PF05724	28 - 210	1.9e-44	Thiopurine S-methyltransferase (TPMT)
Methyltransf_31	PF13847	68 - 164	1.5e-09	Methyltransferase domain
Methyltransf_11	PF08241	68 - 163	1.6e-08	Methyltransferase domain
Methyltransf_25	PF13649	69 - 160	5.6e-11	Methyltransferase domain
Methyltransf_12	PF08242	69 - 162	4.3e-10	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005015)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43910 AT2G43910 AT2G43910 AT2G43920 AT2G43920 AT2G43920 AT2G43920 AT2G43920 AT2G43920 AT2G43940
fragaria_vesca	FvH4_1g00240 FvH4_1g00240 FvH4_1g00240
malus_domestica	MD15G1143500.v1.1
prunus_persica	Prupe.7G269600_v2.0.a1 Prupe.7G269600_v2.0.a1 Prupe.7G269600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0084541
rosa_laevigata	RLG00000015625
rosa_multiflora	Rmu_sc0009647.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155980
rosa_rugosa	Rorug01G0454800 Rorug01G0454900 Rorug01G0455000 Rorug01G0455100
rosa_samantha	Rh2AG002900 Rh2BG002500 Rh2CG003000 Rh2DG003000
rosa_wichuraiana	Rw2G000260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	48, 600
AciI	CCGC	3 cut(s)	42, 87, 527
AcuI	CTGAAG	1 cut(s)	41
AfaI	GTAC	1 cut(s)	332
AfiI	CCNNNNNNNGG	5 cut(s)	184, 189, 209, 327, 629
AgsI	TTSAA	1 cut(s)	422
AjnI	CCWGG	2 cut(s)	202, 573
AloI	GAACNNNNNNTCC	2 cut(s)	600, 632
AluBI	AGCT	4 cut(s)	55, 296, 484, 614
AluI	AGCT	4 cut(s)	55, 296, 484, 614
Alw21I	GWGCWC	2 cut(s)	72, 486
Alw26I	GTCTC	1 cut(s)	666
Alw44I	GTGCAC	1 cut(s)	68
ApaLI	GTGCAC	1 cut(s)	68
ApeKI	GCWGC	1 cut(s)	136
AspS9I	GGNCC	1 cut(s)	524
AvaII	GGWCC	1 cut(s)	524
BaeGI	GKGCMC	2 cut(s)	72, 448
BanII	GRGCYC	3 cut(s)	178, 196, 486
BarI	GAAGNNNNNNTAC	2 cut(s)	323, 355
Bbv12I	GWGCWC	2 cut(s)	72, 486
BbvI	GCAGC	1 cut(s)	148
BccI	CCATC	3 cut(s)	165, 470, 602
BceAI	ACGGC	1 cut(s)	29
BciT130I	CCWGG	2 cut(s)	204, 575
BclI	TGATCA	1 cut(s)	511
BcoDI	GTCTC	1 cut(s)	666
BfaI	CTAG	2 cut(s)	611, 644
BfmI	CTRYAG	1 cut(s)	81
BglII	AGATCT	1 cut(s)	434
BisI	GCNGC	1 cut(s)	137
BlsI	GCNGC	1 cut(s)	138
Bme1390I	CCNGG	2 cut(s)	204, 575
Bme18I	GGWCC	1 cut(s)	524
BmgT120I	GGNCC	1 cut(s)	524
BmiI	GGNNCC	1 cut(s)	175
BmrFI	CCNGG	2 cut(s)	204, 575
BmsI	GCATC	3 cut(s)	13, 376, 577
BpmI	CTGGAG	1 cut(s)	320
BsaI	GGTCTC	1 cut(s)	666
BsaJI	CCNNGG	5 cut(s)	122, 183, 202, 573, 574
BsaWI	WCCGGW	1 cut(s)	472
Bsc4I	CCNNNNNNNGG	5 cut(s)	184, 189, 209, 327, 629
Bse1I	ACTGG	3 cut(s)	220, 265, 507
BseBI	CCWGG	2 cut(s)	204, 575
BseDI	CCNNGG	5 cut(s)	122, 183, 202, 573, 574
BseGI	GGATG	4 cut(s)	212, 367, 481, 568
BseLI	CCNNNNNNNGG	5 cut(s)	184, 189, 209, 327, 629
BseNI	ACTGG	3 cut(s)	220, 265, 507
BseSI	GKGCMC	2 cut(s)	72, 448
BseXI	GCAGC	1 cut(s)	148
BseYI	CCCAGC	1 cut(s)	102
BsiHKAI	GWGCWC	2 cut(s)	72, 486
BsiSI	CCGG	1 cut(s)	473
BslFI	GGGAC	2 cut(s)	107, 185
BslI	CCNNNNNNNGG	5 cut(s)	184, 189, 209, 327, 629
BsmAI	GTCTC	1 cut(s)	666
BsmFI	GGGAC	2 cut(s)	107, 185
BsmI	GAATGC	1 cut(s)	286
Bso31I	GGTCTC	1 cut(s)	666
Bsp1286I	GDGCHC	5 cut(s)	72, 178, 196, 448, 486
Bsp143I	GATC	4 cut(s)	385, 434, 453, 511
BspACI	CCGC	3 cut(s)	42, 87, 527
BspLI	GGNNCC	1 cut(s)	175
BspTNI	GGTCTC	1 cut(s)	666
BsrI	ACTGG	3 cut(s)	220, 265, 507
BssECI	CCNNGG	5 cut(s)	122, 183, 202, 573, 574
BssMI	GATC	4 cut(s)	385, 434, 453, 511
BssT1I	CCWWGG	2 cut(s)	122, 183
Bst2UI	CCWGG	2 cut(s)	204, 575
Bst4CI	ACNGT	1 cut(s)	330
Bst6I	CTCTTC	4 cut(s)	2, 552, 628, 650
BstAPI	GCANNNNNTGC	1 cut(s)	67
BstDEI	CTNAG	1 cut(s)	665
BstENI	CCTNNNNNAGG	1 cut(s)	182
BstF5I	GGATG	4 cut(s)	212, 367, 481, 568
BstKTI	GATC	4 cut(s)	388, 437, 456, 514
BstMAI	GTCTC	1 cut(s)	666
BstMBI	GATC	4 cut(s)	385, 434, 453, 511
BstMWI	GCNNNNNNNGC	2 cut(s)	67, 142
BstNI	CCWGG	2 cut(s)	204, 575
BstNSI	RCATGY	1 cut(s)	243
BstSCI	CCNGG	2 cut(s)	202, 573
BstSFI	CTRYAG	1 cut(s)	81
BstSLI	GKGCMC	2 cut(s)	72, 448
BstV1I	GCAGC	1 cut(s)	148
BstX2I	RGATCY	1 cut(s)	434
BstXI	CCANNNNNNTGG	1 cut(s)	265
BstYI	RGATCY	1 cut(s)	434
BtsCI	GGATG	4 cut(s)	212, 367, 481, 568
BtsIMutI	CAGTG	4 cut(s)	213, 258, 491, 514
Cfr13I	GGNCC	1 cut(s)	524
Csp6I	GTAC	1 cut(s)	331
CviAII	CATG	4 cut(s)	240, 254, 430, 441
CviJI	RGCY	8 cut(s)	55, 139, 176, 194, 296, 484, 579, 614
CviKI_1	RGCY	8 cut(s)	55, 139, 176, 194, 296, 484, 579, 614
CviQI	GTAC	1 cut(s)	331
DdeI	CTNAG	1 cut(s)	665
DpnI	GATC	4 cut(s)	387, 436, 455, 513
DpnII	GATC	4 cut(s)	385, 434, 453, 511
Eam1104I	CTCTTC	4 cut(s)	2, 552, 628, 650
EarI	CTCTTC	4 cut(s)	2, 552, 628, 650
Ecl136II	GAGCTC	1 cut(s)	484
Eco130I	CCWWGG	2 cut(s)	122, 183
Eco24I	GRGCYC	3 cut(s)	178, 196, 486
Eco31I	GGTCTC	1 cut(s)	666
Eco32I	GATATC	1 cut(s)	463
Eco47I	GGWCC	1 cut(s)	524
Eco53kI	GAGCTC	1 cut(s)	484
Eco57I	CTGAAG	1 cut(s)	41
EcoICRI	GAGCTC	1 cut(s)	484
EcoNI	CCTNNNNNAGG	1 cut(s)	182
EcoRII	CCWGG	2 cut(s)	202, 573
EcoRV	GATATC	1 cut(s)	463
EcoT14I	CCWWGG	2 cut(s)	122, 183
EcoT38I	GRGCYC	3 cut(s)	178, 196, 486
ErhI	CCWWGG	2 cut(s)	122, 183
FaeI	CATG	4 cut(s)	243, 257, 433, 444
FaiI	YATR	9 cut(s)	222, 241, 255, 402, 431, 442, 488, 516, 555
FalI	AAGNNNNNCTT	2 cut(s)	338, 370
FaqI	GGGAC	2 cut(s)	107, 185
FatI	CATG	4 cut(s)	239, 253, 429, 440
FauI	CCCGC	1 cut(s)	49
FbaI	TGATCA	1 cut(s)	511
FblI	GTMKAC	2 cut(s)	48, 600
Fnu4HI	GCNGC	1 cut(s)	137
FokI	GGATG	4 cut(s)	219, 354, 488, 555
FriOI	GRGCYC	3 cut(s)	178, 196, 486
Fsp4HI	GCNGC	1 cut(s)	137
FspBI	CTAG	2 cut(s)	611, 644
GluI	GCNGC	1 cut(s)	137
GsaI	CCCAGC	1 cut(s)	106
GsuI	CTGGAG	1 cut(s)	320
HapII	CCGG	1 cut(s)	473
Hin1II	CATG	4 cut(s)	243, 257, 433, 444
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HindIII	AAGCTT	1 cut(s)	53
HpaII	CCGG	1 cut(s)	473
Hpy166II	GTNNAC	5 cut(s)	49, 70, 352, 524, 601
Hpy188I	TCNGA	5 cut(s)	168, 277, 550, 620, 683
Hpy188III	TCNNGA	2 cut(s)	245, 299
Hpy8I	GTNNAC	5 cut(s)	49, 70, 352, 524, 601
Hpy99I	CGWCG	1 cut(s)	50
HpyAV	CCTTC	3 cut(s)	188, 349, 642
HpyCH4III	ACNGT	1 cut(s)	330
HpyCH4IV	ACGT	2 cut(s)	250, 404
HpyCH4V	TGCA	4 cut(s)	70, 239, 440, 568
HpyF10VI	GCNNNNNNNGC	2 cut(s)	67, 142
HpyF3I	CTNAG	1 cut(s)	665
HpySE526I	ACGT	2 cut(s)	250, 404
Hsp92II	CATG	4 cut(s)	243, 257, 433, 444
Ksp22I	TGATCA	1 cut(s)	511
Kzo9I	GATC	4 cut(s)	385, 434, 453, 511
LmnI	GCTCC	3 cut(s)	106, 173, 481
Lsp1109I	GCAGC	1 cut(s)	148
LweI	GCATC	3 cut(s)	13, 376, 577
MaeI	CTAG	2 cut(s)	611, 644
MaeII	ACGT	2 cut(s)	250, 404
MalI	GATC	4 cut(s)	387, 436, 455, 513
MboI	GATC	4 cut(s)	385, 434, 453, 511
MboII	GAAGA	9 cut(s)	19, 29, 32, 152, 400, 463, 569, 645, 667
MflI	RGATCY	1 cut(s)	434
MhlI	GDGCHC	5 cut(s)	72, 178, 196, 448, 486
MluCI	AATT	2 cut(s)	234, 377
MmeI	TCCRAC	2 cut(s)	395, 643
MnlI	CCTC	7 cut(s)	7, 48, 322, 553, 601, 633, 651
MseI	TTAA	4 cut(s)	288, 344, 467, 582
MslI	CAYNNNNRTG	1 cut(s)	519
MspI	CCGG	1 cut(s)	473
MspR9I	CCNGG	2 cut(s)	204, 575
Mva1269I	GAATGC	1 cut(s)	286
MvaI	CCWGG	2 cut(s)	204, 575
MwoI	GCNNNNNNNGC	2 cut(s)	67, 142
NdeII	GATC	4 cut(s)	385, 434, 453, 511
NlaIII	CATG	4 cut(s)	243, 257, 433, 444
NlaIV	GGNNCC	1 cut(s)	175
NspI	RCATGY	1 cut(s)	243
PasI	CCCWGGG	1 cut(s)	574
PctI	GAATGC	1 cut(s)	286
PkrI	GCNGC	1 cut(s)	138
Psp124BI	GAGCTC	1 cut(s)	486
Psp6I	CCWGG	2 cut(s)	202, 573
PspFI	CCCAGC	1 cut(s)	102
PspGI	CCWGG	2 cut(s)	202, 573
PspN4I	GGNNCC	1 cut(s)	175
PspPI	GGNCC	1 cut(s)	524
PsuI	RGATCY	1 cut(s)	434
RsaI	GTAC	1 cut(s)	332
RsaNI	GTAC	1 cut(s)	331
RseI	CAYNNNNRTG	1 cut(s)	519
SacI	GAGCTC	1 cut(s)	486
SalI	GTCGAC	1 cut(s)	47
SaqAI	TTAA	4 cut(s)	288, 344, 467, 582
SatI	GCNGC	1 cut(s)	137
Sau3AI	GATC	4 cut(s)	385, 434, 453, 511
Sau96I	GGNCC	1 cut(s)	524
ScrFI	CCNGG	2 cut(s)	204, 575
SduI	GDGCHC	5 cut(s)	72, 178, 196, 448, 486
SfaNI	GCATC	3 cut(s)	13, 376, 577
SfcI	CTRYAG	1 cut(s)	81
SinI	GGWCC	1 cut(s)	524
SmiMI	CAYNNNNRTG	1 cut(s)	519
Sse9I	AATT	2 cut(s)	234, 377
SsiI	CCGC	3 cut(s)	42, 87, 527
SspMI	CTAG	2 cut(s)	611, 644
SstI	GAGCTC	1 cut(s)	486
StyD4I	CCNGG	2 cut(s)	202, 573
StyI	CCWWGG	2 cut(s)	122, 183
TaaI	ACNGT	1 cut(s)	330
TaiI	ACGT	2 cut(s)	253, 407
TaqI	TCGA	1 cut(s)	48
TasI	AATT	2 cut(s)	234, 377
Tru1I	TTAA	4 cut(s)	288, 344, 467, 582
Tru9I	TTAA	4 cut(s)	288, 344, 467, 582
TscAI	CASTG	4 cut(s)	220, 265, 498, 514
TseI	GCWGC	1 cut(s)	136
TspDTI	ATGAA	3 cut(s)	152, 570, 621
TspGWI	ACGGA	1 cut(s)	463
TspRI	CASTG	4 cut(s)	220, 265, 498, 514
VneI	GTGCAC	1 cut(s)	68
VpaK11BI	GGWCC	1 cut(s)	524
XagI	CCTNNNNNAGG	1 cut(s)	182
XceI	RCATGY	1 cut(s)	243
XmiI	GTMKAC	2 cut(s)	48, 600
XspI	CTAG	2 cut(s)	611, 644

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.