Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010133

Physical Location & Seq

Genomic Coordinates Forward (+)

23850165 .. 23852924

Gene Structure 2760 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1273400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGGGTCGCGTGGCTTCAAACCCGGTTAGCCTCCATAGATCCCGGCCATGTTTCCAAACTCCTCGACTTCCCCACTACGTTAAGCCCACTCTCCACTTCCCAAACCACCACCGCCGATTTCCGACCGTTTCTTGCCAGCAAACCCCCAACCCACCTGACCCCTCCACAACGGAGAAGTCGGTTGCTGAGCCAGGCTCTGTCAGTGATAGCAGTAGTCAAGCTACGACTTCCTCTACGAGCTCTGGCCCTCCGGAATTTCCAAACAAGAATACCAACAGGCAAGTAGCGGTGGTTTCCACTCTTGCGGCGGTCGTGCTTTTCTTATCAGGAAGACTAGATTTTGGTGTTTCTTTGAAGGACCTATCTCTTGCTGCATTACCTTATGAAGAGGCTCTTTCGAATGGGAAGCCTACTGTTGTCGAGTTCTATGCCGATTGGTGTGAAGTATGCAAGGAATTAGCTCCAGATGTGTATAAAGTTGAGCAGCAGTACAAGGATCGTGTAAATTTTGTTATGCTGAATGTTGACAACACAAAGTGGGAACAAGAGCTTGATGAGTTTGGTGTTGAGGGTATTCCACACTTTGCATTCCTAGATAAAGCGGGAAATGAAGAGGGGAACGTAGTAGGCAGGCTTCCAGAAAAGTACCTGCTTGAGAATGTGGATGCTCTTGCCCGTGGAGAAGCCTCCATACCTCATGCTCGTGTAGTGGGGCAGTTTTCAAGTGCTGAAGCCAGAAAGGTTCACCAAGTTCCTGATCCAAGAAGTCACGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009636 GO:0009987 GO:0010190 GO:0015035 GO:0015036 GO:0016020 GO:0016043 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0016671 GO:0017004 GO:0019725 GO:0022607 GO:0031976 GO:0031977 GO:0031984 GO:0033554 GO:0034357 GO:0034599 GO:0034622 GO:0042221 GO:0042592 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045454 GO:0047134 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055035 GO:0055114 GO:0065003 GO:0065007 GO:0065008 GO:0070887 GO:0071840 GO:0097237 GO:0098754 GO:0098869 GO:1990748

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Thioredoxin

Protein Analysis

259

Amino Acids

28.42

Weight (kDa)

5.99

Isoelectric Point (pI)

32.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thioredoxin	PF00085	127 - 222	3.6e-20	Thioredoxin
Thioredoxin_7	PF13899	128 - 199	4.1e-10	Thioredoxin-like
Thioredoxin_2	PF13098	133 - 217	1.8e-09	Thioredoxin-like domain
Thioredoxin_8	PF13905	135 - 187	4.5e-07	Thioredoxin-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37200
fragaria_vesca	FvH4_1g14520
malus_domestica	MD02G1158600.v1.1 MD15G1273400.v1.1 MD17G1248400.v1.1
prunus_persica	Prupe.7G145200_v2.0.a1
pyrus_communis	pycom02g12510 pycom15g23650 pycom17g24960 pycom17g25120
rosa_chinensis	RchiOBHm_Chr2g0103261
rosa_laevigata	RLG00000017219
rosa_multiflora	Rmu_sc0006743.1_g000012
rosa_roxburghii	Rroxscaffold_2G00139800
rosa_rugosa	Rorug02G0112900 Rorug02G0113000
rosa_samantha	Rh2AG161600 Rh2BG168500 Rh2CG168500 Rh2DG167400
rosa_wichuraiana	Rw2G012690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	657
AccII	CGCG	1 cut(s)	9
AccIII	TCCGGA	1 cut(s)	250
AciI	CCGC	5 cut(s)	112, 287, 305, 308, 602
AclWI	GGATC	3 cut(s)	33, 504, 752
AcoI	YGGCCR	1 cut(s)	44
AcsI	RAATTY	2 cut(s)	254, 505
AcuI	CTGAAG	1 cut(s)	750
AdeI	CACNNNGTG	1 cut(s)	537
AfaI	GTAC	2 cut(s)	491, 647
AgsI	TTSAA	3 cut(s)	18, 355, 723
AjnI	CCWGG	1 cut(s)	190
AluBI	AGCT	4 cut(s)	221, 240, 461, 550
AluI	AGCT	4 cut(s)	221, 240, 461, 550
Alw21I	GWGCWC	1 cut(s)	242
AlwI	GGATC	3 cut(s)	33, 504, 752
AlwNI	CAGNNNCTG	1 cut(s)	197
Aor13HI	TCCGGA	1 cut(s)	250
AoxI	GGCC	2 cut(s)	44, 244
ApeKI	GCWGC	2 cut(s)	371, 484
ApoI	RAATTY	2 cut(s)	254, 505
AspS9I	GGNCC	2 cut(s)	245, 358
AsuC2I	CCSGG	2 cut(s)	23, 43
AsuHPI	GGTGA	1 cut(s)	737
AsuII	TTCGAA	1 cut(s)	398
AvaII	GGWCC	1 cut(s)	358
BanII	GRGCYC	1 cut(s)	242
BarI	GAAGNNNNNNTAC	4 cut(s)	618, 650, 675, 707
BauI	CACGAG	1 cut(s)	702
BbsI	GAAGAC	1 cut(s)	337
Bbv12I	GWGCWC	1 cut(s)	242
BbvI	GCAGC	2 cut(s)	358, 496
BcgI	CGANNNNNNTGC	2 cut(s)	410, 444
BciT130I	CCWGG	1 cut(s)	192
BcnI	CCSGG	2 cut(s)	23, 43
BfaI	CTAG	2 cut(s)	335, 593
BfuAI	ACCTGC	1 cut(s)	657
BisI	GCNGC	3 cut(s)	306, 372, 485
BlpI	GCTNAGC	1 cut(s)	186
BlsI	GCNGC	3 cut(s)	307, 373, 486
Bme1390I	CCNGG	3 cut(s)	23, 43, 192
Bme18I	GGWCC	1 cut(s)	358
BmgT120I	GGNCC	2 cut(s)	245, 358
BmrFI	CCNGG	3 cut(s)	23, 43, 192
BmsI	GCATC	1 cut(s)	655
BpiI	GAAGAC	1 cut(s)	337
BplI	GAGNNNNNCTC	2 cut(s)	179, 211
BpmI	CTGGAG	1 cut(s)	447
Bpu1102I	GCTNAGC	1 cut(s)	186
Bpu14I	TTCGAA	1 cut(s)	398
BpuEI	CTTGAG	1 cut(s)	674
BpuMI	CCSGG	2 cut(s)	23, 43
BsaJI	CCNNGG	1 cut(s)	676
BsaWI	WCCGGW	1 cut(s)	250
BseAI	TCCGGA	1 cut(s)	250
BseBI	CCWGG	1 cut(s)	192
BseDI	CCNNGG	1 cut(s)	676
BseGI	GGATG	1 cut(s)	670
BseMII	CTCAG	1 cut(s)	177
BseRI	GAGGAG	1 cut(s)	51
BseXI	GCAGC	2 cut(s)	358, 496
Bsh1236I	CGCG	1 cut(s)	9
Bsh1285I	CGRYCG	2 cut(s)	126, 312
BshFI	GGCC	2 cut(s)	46, 246
BsiEI	CGRYCG	2 cut(s)	126, 312
BsiHKAI	GWGCWC	1 cut(s)	242
BsiSI	CCGG	3 cut(s)	23, 43, 251
BsmI	GAATGC	1 cut(s)	587
BsnI	GGCC	2 cut(s)	46, 246
Bsp119I	TTCGAA	1 cut(s)	398
Bsp1286I	GDGCHC	1 cut(s)	242
Bsp13I	TCCGGA	1 cut(s)	250
Bsp143I	GATC	3 cut(s)	38, 496, 757
Bsp1720I	GCTNAGC	1 cut(s)	186
BspACI	CCGC	5 cut(s)	112, 287, 305, 308, 602
BspANI	GGCC	2 cut(s)	46, 246
BspCNI	CTCAG	1 cut(s)	178
BspEI	TCCGGA	1 cut(s)	250
BspFNI	CGCG	1 cut(s)	9
BspMI	ACCTGC	1 cut(s)	657
BspPI	GGATC	3 cut(s)	33, 504, 752
BspT104I	TTCGAA	1 cut(s)	398
BssECI	CCNNGG	1 cut(s)	676
BssMI	GATC	3 cut(s)	38, 496, 757
BssSI	CACGAG	1 cut(s)	702
Bst2BI	CACGAG	1 cut(s)	702
Bst2UI	CCWGG	1 cut(s)	192
Bst4CI	ACNGT	2 cut(s)	127, 415
Bst6I	CTCTTC	2 cut(s)	381, 606
BstBI	TTCGAA	1 cut(s)	398
BstC8I	GCNNGC	2 cut(s)	137, 632
BstDEI	CTNAG	1 cut(s)	186
BstDSI	CCRYGG	1 cut(s)	676
BstF5I	GGATG	1 cut(s)	670
BstFNI	CGCG	1 cut(s)	9
BstKTI	GATC	3 cut(s)	41, 499, 760
BstMBI	GATC	3 cut(s)	38, 496, 757
BstMCI	CGRYCG	2 cut(s)	126, 312
BstNI	CCWGG	1 cut(s)	192
BstSCI	CCNGG	3 cut(s)	21, 41, 190
BstUI	CGCG	1 cut(s)	9
BstV1I	GCAGC	2 cut(s)	358, 496
BstV2I	GAAGAC	1 cut(s)	337
BstX2I	RGATCY	1 cut(s)	38
BstYI	RGATCY	1 cut(s)	38
BsuRI	GGCC	2 cut(s)	46, 246
BtgI	CCRYGG	1 cut(s)	676
BtsCI	GGATG	1 cut(s)	670
BtsIMutI	CAGTG	1 cut(s)	208
BveI	ACCTGC	1 cut(s)	657
Cac8I	GCNNGC	2 cut(s)	137, 632
CaiI	CAGNNNCTG	1 cut(s)	197
Cfr13I	GGNCC	2 cut(s)	245, 358
Csp6I	GTAC	2 cut(s)	490, 646
CviAII	CATG	2 cut(s)	48, 698
CviQI	GTAC	2 cut(s)	490, 646
DdeI	CTNAG	1 cut(s)	186
DpnI	GATC	3 cut(s)	40, 498, 759
DpnII	GATC	3 cut(s)	38, 496, 757
DraIII	CACNNNGTG	1 cut(s)	537
EaeI	YGGCCR	1 cut(s)	44
Eam1104I	CTCTTC	2 cut(s)	381, 606
EarI	CTCTTC	2 cut(s)	381, 606
Ecl136II	GAGCTC	1 cut(s)	240
Eco24I	GRGCYC	1 cut(s)	242
Eco47I	GGWCC	1 cut(s)	358
Eco53kI	GAGCTC	1 cut(s)	240
Eco57I	CTGAAG	1 cut(s)	750
EcoICRI	GAGCTC	1 cut(s)	240
EcoO109I	RGGNCCY	1 cut(s)	358
EcoRII	CCWGG	1 cut(s)	190
EcoT38I	GRGCYC	1 cut(s)	242
FaeI	CATG	2 cut(s)	51, 701
FaiI	YATR	9 cut(s)	36, 49, 384, 429, 448, 474, 515, 692, 699
FalI	AAGNNNNNCTT	2 cut(s)	378, 410
FatI	CATG	2 cut(s)	47, 697
FauI	CCCGC	1 cut(s)	595
Fnu4HI	GCNGC	3 cut(s)	306, 372, 485
FokI	GGATG	1 cut(s)	677
FriOI	GRGCYC	1 cut(s)	242
Fsp4HI	GCNGC	3 cut(s)	306, 372, 485
FspBI	CTAG	2 cut(s)	335, 593
GluI	GCNGC	3 cut(s)	306, 372, 485
GsuI	CTGGAG	1 cut(s)	447
HaeIII	GGCC	2 cut(s)	46, 246
HapII	CCGG	3 cut(s)	23, 43, 251
Hin1II	CATG	2 cut(s)	51, 701
HincII	GTYRAC	1 cut(s)	526
HindII	GTYRAC	1 cut(s)	526
HpaII	CCGG	3 cut(s)	23, 43, 251
HphI	GGTGA	1 cut(s)	737
Hpy166II	GTNNAC	2 cut(s)	526, 745
Hpy188I	TCNGA	1 cut(s)	123
Hpy188III	TCNNGA	5 cut(s)	251, 327, 464, 638, 755
Hpy8I	GTNNAC	2 cut(s)	526, 745
HpyAV	CCTTC	1 cut(s)	349
HpyCH4III	ACNGT	2 cut(s)	127, 415
HpyCH4IV	ACGT	2 cut(s)	78, 621
HpyCH4V	TGCA	3 cut(s)	374, 450, 587
HpyF3I	CTNAG	1 cut(s)	186
HpySE526I	ACGT	2 cut(s)	78, 621
Hsp92II	CATG	2 cut(s)	51, 701
Kpn2I	TCCGGA	1 cut(s)	250
Kzo9I	GATC	3 cut(s)	38, 496, 757
LmnI	GCTCC	1 cut(s)	466
Lsp1109I	GCAGC	2 cut(s)	358, 496
LweI	GCATC	1 cut(s)	655
MaeI	CTAG	2 cut(s)	335, 593
MaeII	ACGT	2 cut(s)	78, 621
MaeIII	GTNAC	1 cut(s)	767
MalI	GATC	3 cut(s)	40, 498, 759
MboI	GATC	3 cut(s)	38, 496, 757
MboII	GAAGA	3 cut(s)	342, 398, 623
MflI	RGATCY	1 cut(s)	38
MhlI	GDGCHC	1 cut(s)	242
MluCI	AATT	3 cut(s)	254, 455, 505
MmeI	TCCRAC	1 cut(s)	146
MroI	TCCGGA	1 cut(s)	250
MseI	TTAA	1 cut(s)	81
MslI	CAYNNNNRTG	1 cut(s)	702
MspI	CCGG	3 cut(s)	23, 43, 251
MspR9I	CCNGG	3 cut(s)	23, 43, 192
Mva1269I	GAATGC	1 cut(s)	587
MvaI	CCWGG	1 cut(s)	192
MvnI	CGCG	1 cut(s)	9
NciI	CCSGG	2 cut(s)	23, 43
NdeII	GATC	3 cut(s)	38, 496, 757
NlaIII	CATG	2 cut(s)	51, 701
NmuCI	GTSAC	1 cut(s)	767
NspV	TTCGAA	1 cut(s)	398
PctI	GAATGC	1 cut(s)	587
PkrI	GCNGC	3 cut(s)	307, 373, 486
PpuMI	RGGWCCY	1 cut(s)	358
Psp124BI	GAGCTC	1 cut(s)	242
Psp5II	RGGWCCY	1 cut(s)	358
Psp6I	CCWGG	1 cut(s)	190
PspGI	CCWGG	1 cut(s)	190
PspPI	GGNCC	2 cut(s)	245, 358
PspPPI	RGGWCCY	1 cut(s)	358
PstNI	CAGNNNCTG	1 cut(s)	197
PsuI	RGATCY	1 cut(s)	38
RsaI	GTAC	2 cut(s)	491, 647
RsaNI	GTAC	2 cut(s)	490, 646
RseI	CAYNNNNRTG	1 cut(s)	702
SacI	GAGCTC	1 cut(s)	242
SaqAI	TTAA	1 cut(s)	81
SatI	GCNGC	3 cut(s)	306, 372, 485
Sau3AI	GATC	3 cut(s)	38, 496, 757
Sau96I	GGNCC	2 cut(s)	245, 358
ScrFI	CCNGG	3 cut(s)	23, 43, 192
SduI	GDGCHC	1 cut(s)	242
SfaNI	GCATC	1 cut(s)	655
SfuI	TTCGAA	1 cut(s)	398
SinI	GGWCC	1 cut(s)	358
SmiMI	CAYNNNNRTG	1 cut(s)	702
SmlI	CTYRAG	1 cut(s)	653
SmoI	CTYRAG	1 cut(s)	653
Sse9I	AATT	3 cut(s)	254, 455, 505
SsiI	CCGC	5 cut(s)	112, 287, 305, 308, 602
SspMI	CTAG	2 cut(s)	335, 593
SstI	GAGCTC	1 cut(s)	242
StyD4I	CCNGG	3 cut(s)	21, 41, 190
TaaI	ACNGT	2 cut(s)	127, 415
TaiI	ACGT	2 cut(s)	81, 624
TaqI	TCGA	3 cut(s)	64, 398, 420
TasI	AATT	3 cut(s)	254, 455, 505
TatI	WGTACW	1 cut(s)	489
TauI	GCSGC	1 cut(s)	308
Tru1I	TTAA	1 cut(s)	81
Tru9I	TTAA	1 cut(s)	81
TscAI	CASTG	1 cut(s)	208
TseFI	GTSAC	1 cut(s)	767
TseI	GCWGC	2 cut(s)	371, 484
Tsp45I	GTSAC	1 cut(s)	767
TspDTI	ATGAA	2 cut(s)	399, 624
TspGWI	ACGGA	1 cut(s)	185
TspRI	CASTG	1 cut(s)	208
VpaK11BI	GGWCC	1 cut(s)	358
XapI	RAATTY	2 cut(s)	254, 505
XspI	CTAG	2 cut(s)	335, 593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.