Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0010133

Physical Location & Seq

Genomic Coordinates Forward (+)

9650741 .. 9653788

Gene Structure 3048 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0112900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGGGAAAGATGTCTCCCTCAGTACGTTCACTAGTATCAACCTCCCTCATCAGAAAGCCACCTTCTTTATCCCCAAACCAATCCCCATCTCATAAACCCCATAACTCTCAGACCCACCTCACCAGAAAAACCCCACCTCAAAATTCCGGCAAAACCCAACTCCAACTCCAACTCCAAACCCAGACCAAGAACCCATTCACCTCCCCAAACCTCTCAGACGCCAAAAGGGTCTTCAACTCCATCGCCACCGCAACAAAAACCCCTCTCGATCTCCGCGTCCACAACGCCCTCCTCCAATCCTACGCCGCAATCTCCACCGTCAACGACTCCATCGCTCTCTTCAACCACATGATCAAAACCCAACCCGCATTCTCGCCGGACCGGTCCACCTACCACATCTTGCTCGCCCAGTCCTGCACCGTACCCGATCAGACCCTCCAATCTGTTCACCAAGTCCTCAACTTTATGCTCAACAATGGCTTCCACCCAGATAAGGTCACCACCGACATTGCCGTCCGGTCACTCTGCTTGGCCGGACACGTGGACGAGGCAGTGCAAGTGATGAAGCAAATGTCGTTGAAAAACGCGGTACCGGATACTTATACTTACAATTTTCTTGTCAAGTGTCTCTGCAAGTCGCGAGTTTTGAGTACTGTTAAAGAGTTTATTGATGATATGCGAAAGTCTTTCGATTTGAAGCCGGATTTGGTTACTTATACGATTATGATTGACAATGTAGCTAATAGCAAGAATTTACGTGAGGCTAGCCGATTAGTGAAGGAGTTAGATAAGGAGGGGTTTAAGCCGGATTGCTATGTTTACAATACGATTATGAAAGGCTATTGTATGCTGAGTAAGGGTAATGAGGTGGTTCAGGTGTTGAAGGACATGCAGGAGAATGGAGTGGAGCCTGATCTTGTTACATATAATACTCTGATTTTCGGGTTGTCGAAGTGTGGCAGGGTTATGGAGGCGAAGAAGTATTTGCGGATCATGGTGGACATGGGGCATTTCCCCAACGAGGTGACTTATACTTCATTGATGAATGGATTGTGTCGGAAGGGGGATGCATTGGGTGCAGTGGCATTGTTGGAGGAGATGCAAGCGCAGGGGTGCAGCCCGAATGAGTGCACCTATAATACCTTGCTTCATGGGTTGTGTAAGGGAAGGTTGTTAGAGAAAGGGCTGGAATTGTATGGACTGATGAAAAAAGATGGGATGAAGCTTGACACGGCTTCGTATAGTACACTTGTGAGGACACTTTGTAGGGCAGGCAGGGTTGCAGACGCTTATGGAGTGTTTGATTATGCAGTTGAGAGTAAGAGCTTAACAAATGTTTCTGCTTACTTGACTTTGGAAAGTACAGTTAAGCAGCTTAAGAAAGCACGACCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009636 GO:0009987 GO:0010190 GO:0015035 GO:0015036 GO:0016020 GO:0016043 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0016671 GO:0017004 GO:0019725 GO:0022607 GO:0031976 GO:0031977 GO:0031984 GO:0033554 GO:0034357 GO:0034599 GO:0034622 GO:0042221 GO:0042592 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045454 GO:0047134 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055035 GO:0055114 GO:0065003 GO:0065007 GO:0065008 GO:0070887 GO:0071840 GO:0097237 GO:0098754 GO:0098869 GO:1990748

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Thioredoxin

Protein Analysis

464

Amino Acids

51.66

Weight (kDa)

9.16

Isoelectric Point (pI)

36.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_1	PF12854	159 - 192	6.4e-06	PPR repeat
PPR_2	PF13041	174 - 212	4.6e-09	PPR repeat family
PPR_3	PF13812	230 - 281	1.7e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	234 - 282	2.4e-11	PPR repeat family
PPR_3	PF13812	257 - 313	2.2e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	269 - 318	4.1e-14	PPR repeat family
PPR_1	PF12854	300 - 332	4.3e-10	PPR repeat
PPR_2	PF13041	304 - 353	1.9e-15	PPR repeat family
PPR	PF01535	307 - 336	3.4e-06	PPR repeat
PPR_long	PF17177	309 - 439	7.1e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	332 - 384	1.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	335 - 368	9.7e-11	PPR repeat
PPR	PF01535	342 - 372	1.7e-07	PPR repeat
PPR_2	PF13041	342 - 388	6.2e-17	PPR repeat family
PPR_1	PF12854	371 - 402	2.1e-08	PPR repeat
PPR_2	PF13041	377 - 423	1.2e-11	PPR repeat family
PPR	PF01535	378 - 406	6.5e-07	PPR repeat
PPR_1	PF12854	405 - 436	3.8e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37200
fragaria_vesca	FvH4_1g14520
malus_domestica	MD02G1158600.v1.1 MD15G1273400.v1.1 MD17G1248400.v1.1
prunus_persica	Prupe.7G145200_v2.0.a1
pyrus_communis	pycom02g12510 pycom15g23650 pycom17g24960 pycom17g25120
rosa_chinensis	RchiOBHm_Chr2g0103261
rosa_laevigata	RLG00000017219
rosa_multiflora	Rmu_sc0006743.1_g000012
rosa_roxburghii	Rroxscaffold_2G00139800
rosa_rugosa	Rorug02G0112900 Rorug02G0113000
rosa_samantha	Rh2AG161600 Rh2BG168500 Rh2CG168500 Rh2DG167400
rosa_wichuraiana	Rw2G012690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	512
Acc65I	GGTACC	1 cut(s)	589
AccB1I	GGYRCC	1 cut(s)	589
AccII	CGCG	3 cut(s)	276, 587, 640
AciI	CCGC	6 cut(s)	249, 274, 306, 366, 587, 988
AclWI	GGATC	1 cut(s)	998
AcoI	YGGCCR	1 cut(s)	531
AcsI	RAATTY	2 cut(s)	142, 751
AcvI	CACGTG	1 cut(s)	541
AcyI	GRCGYC	1 cut(s)	219
AfaI	GTAC	6 cut(s)	24, 423, 591, 652, 1246, 1363
AfiI	CCNNNNNNNGG	2 cut(s)	493, 1021
AflII	CTTAAG	1 cut(s)	1376
AflIII	ACRYGT	1 cut(s)	538
AgeI	ACCGGT	1 cut(s)	381
AgsI	TTSAA	5 cut(s)	235, 343, 580, 697, 883
AhlI	ACTAGT	1 cut(s)	31
AjuI	GAANNNNNNNTTGG	6 cut(s)	179, 211, 215, 247, 689, 721
AluBI	AGCT	4 cut(s)	740, 1225, 1326, 1375
AluI	AGCT	4 cut(s)	740, 1225, 1326, 1375
Alw21I	GWGCWC	1 cut(s)	1133
Alw26I	GTCTC	2 cut(s)	18, 632
Alw44I	GTGCAC	1 cut(s)	1129
AlwI	GGATC	1 cut(s)	998
AoxI	GGCC	1 cut(s)	531
ApaLI	GTGCAC	1 cut(s)	1129
ApeKI	GCWGC	2 cut(s)	1116, 1372
ApoI	RAATTY	2 cut(s)	142, 751
AsiGI	ACCGGT	1 cut(s)	381
Asp700I	GAANNNNTTC	1 cut(s)	194
Asp718I	GGTACC	1 cut(s)	589
AspLEI	GCGC	1 cut(s)	1108
AspS9I	GGNCC	2 cut(s)	379, 384
AsuHPI	GGTGA	5 cut(s)	112, 190, 440, 490, 1036
AsuNHI	GCTAGC	1 cut(s)	764
AvaII	GGWCC	2 cut(s)	379, 384
BaeGI	GKGCMC	1 cut(s)	1133
BanI	GGYRCC	1 cut(s)	589
BbrPI	CACGTG	1 cut(s)	541
BbsI	GAAGAC	1 cut(s)	223
Bbv12I	GWGCWC	1 cut(s)	1133
BbvI	GCAGC	2 cut(s)	1128, 1384
BccI	CCATC	4 cut(s)	94, 248, 338, 1208
BceAI	ACGGC	2 cut(s)	497, 1248
BciVI	GTATCC	1 cut(s)	589
BclI	TGATCA	1 cut(s)	351
BcoDI	GTCTC	2 cut(s)	18, 632
BcuI	ACTAGT	1 cut(s)	31
BfaI	CTAG	2 cut(s)	32, 765
BfrI	CTTAAG	1 cut(s)	1376
BfuI	GTATCC	1 cut(s)	589
BisI	GCNGC	3 cut(s)	306, 1117, 1373
BlsI	GCNGC	3 cut(s)	307, 1118, 1374
BmcAI	AGTACT	1 cut(s)	652
Bme18I	GGWCC	2 cut(s)	379, 384
BmgT120I	GGNCC	2 cut(s)	379, 384
BmiI	GGNNCC	2 cut(s)	591, 909
BmrI	ACTGGG	1 cut(s)	403
BmsI	GCATC	2 cut(s)	1057, 1089
BmtI	GCTAGC	1 cut(s)	768
BmuI	ACTGGG	1 cut(s)	403
BpiI	GAAGAC	1 cut(s)	223
BsaAI	YACGTR	2 cut(s)	541, 758
BsaHI	GRCGYC	1 cut(s)	219
BsaWI	WCCGGW	3 cut(s)	381, 516, 592
BsaXI	ACNNNNNCTCC	4 cut(s)	150, 156, 180, 186
Bsc4I	CCNNNNNNNGG	2 cut(s)	493, 1021
Bse118I	RCCGGY	1 cut(s)	381
Bse1I	ACTGG	1 cut(s)	409
Bse3DI	GCAATG	1 cut(s)	507
BseGI	GGATG	2 cut(s)	1072, 1224
BseLI	CCNNNNNNNGG	2 cut(s)	493, 1021
BseMI	GCAATG	1 cut(s)	507
BseMII	CTCAG	4 cut(s)	33, 122, 228, 842
BseNI	ACTGG	1 cut(s)	409
BseRI	GAGGAG	2 cut(s)	281, 1109
BseSI	GKGCMC	1 cut(s)	1133
BseXI	GCAGC	2 cut(s)	1128, 1384
BsgI	GTGCAG	3 cut(s)	400, 1098, 1135
Bsh1236I	CGCG	3 cut(s)	276, 587, 640
BshFI	GGCC	1 cut(s)	533
BshNI	GGYRCC	1 cut(s)	589
BshTI	ACCGGT	1 cut(s)	381
BsiHKAI	GWGCWC	1 cut(s)	1133
BsiSI	CCGG	8 cut(s)	147, 377, 382, 517, 534, 593, 701, 806
BslI	CCNNNNNNNGG	2 cut(s)	493, 1021
BsmAI	GTCTC	2 cut(s)	18, 632
BsmI	GAATGC	1 cut(s)	368
BsnI	GGCC	1 cut(s)	533
Bsp1286I	GDGCHC	1 cut(s)	1133
Bsp143I	GATC	5 cut(s)	268, 351, 427, 913, 990
Bsp68I	TCGCGA	1 cut(s)	640
BspACI	CCGC	6 cut(s)	249, 274, 306, 366, 587, 988
BspANI	GGCC	1 cut(s)	533
BspCNI	CTCAG	4 cut(s)	32, 121, 227, 843
BspFNI	CGCG	3 cut(s)	276, 587, 640
BspLI	GGNNCC	2 cut(s)	591, 909
BspOI	GCTAGC	1 cut(s)	768
BspPI	GGATC	1 cut(s)	998
BspT107I	GGYRCC	1 cut(s)	589
BspTI	CTTAAG	1 cut(s)	1376
BsrDI	GCAATG	1 cut(s)	507
BsrFI	RCCGGY	1 cut(s)	381
BsrI	ACTGG	1 cut(s)	409
BssAI	RCCGGY	1 cut(s)	381
BssMI	GATC	5 cut(s)	268, 351, 427, 913, 990
BssNI	GRCGYC	1 cut(s)	219
Bst4CI	ACNGT	4 cut(s)	319, 421, 655, 1366
Bst6I	CTCTTC	1 cut(s)	344
BstACI	GRCGYC	1 cut(s)	219
BstAFI	CTTAAG	1 cut(s)	1376
BstAPI	GCANNNNNTGC	1 cut(s)	1076
BstBAI	YACGTR	2 cut(s)	541, 758
BstC8I	GCNNGC	4 cut(s)	405, 766, 1104, 1273
BstDEI	CTNAG	4 cut(s)	19, 108, 214, 851
BstEII	GGTNACC	1 cut(s)	496
BstF5I	GGATG	2 cut(s)	1072, 1224
BstFNI	CGCG	3 cut(s)	276, 587, 640
BstHHI	GCGC	1 cut(s)	1108
BstKTI	GATC	5 cut(s)	271, 354, 430, 916, 993
BstMAI	GTCTC	2 cut(s)	18, 632
BstMBI	GATC	5 cut(s)	268, 351, 427, 913, 990
BstMWI	GCNNNNNNNGC	1 cut(s)	1076
BstNSI	RCATGY	1 cut(s)	892
BstPI	GGTNACC	1 cut(s)	496
BstSLI	GKGCMC	1 cut(s)	1133
BstUI	CGCG	3 cut(s)	276, 587, 640
BstV1I	GCAGC	2 cut(s)	1128, 1384
BstV2I	GAAGAC	1 cut(s)	223
BsuI	GTATCC	1 cut(s)	589
BsuRI	GGCC	1 cut(s)	533
BtgZI	GCGATG	2 cut(s)	226, 316
BtsCI	GGATG	2 cut(s)	1072, 1224
BtsI	GCAGTG	2 cut(s)	558, 1086
BtsIMutI	CAGTG	2 cut(s)	558, 1086
BtuMI	TCGCGA	1 cut(s)	640
Cac8I	GCNNGC	4 cut(s)	405, 766, 1104, 1273
CfoI	GCGC	1 cut(s)	1108
Cfr10I	RCCGGY	1 cut(s)	381
Cfr13I	GGNCC	2 cut(s)	379, 384
CpoI	CGGWCCG	1 cut(s)	379
CseI	GACGC	3 cut(s)	227, 265, 1295
Csp6I	GTAC	6 cut(s)	23, 422, 590, 651, 1245, 1362
CspAI	ACCGGT	1 cut(s)	381
CspCI	CAANNNNNGTGG	2 cut(s)	490, 525
CspI	CGGWCCG	1 cut(s)	379
CviAII	CATG	5 cut(s)	349, 889, 994, 1003, 1151
CviQI	GTAC	6 cut(s)	23, 422, 590, 651, 1245, 1362
DdeI	CTNAG	4 cut(s)	19, 108, 214, 851
DpnI	GATC	5 cut(s)	270, 353, 429, 915, 992
DpnII	GATC	5 cut(s)	268, 351, 427, 913, 990
DrdI	GACNNNNNNGTC	1 cut(s)	512
DseDI	GACNNNNNNGTC	1 cut(s)	512
EaeI	YGGCCR	1 cut(s)	531
Eam1104I	CTCTTC	1 cut(s)	344
EarI	CTCTTC	1 cut(s)	344
Eco47I	GGWCC	2 cut(s)	379, 384
Eco72I	CACGTG	1 cut(s)	541
Eco91I	GGTNACC	1 cut(s)	496
EcoO65I	GGTNACC	1 cut(s)	496
EcoT22I	ATGCAT	1 cut(s)	1072
FaeI	CATG	5 cut(s)	352, 892, 997, 1006, 1154
FatI	CATG	5 cut(s)	348, 888, 993, 1002, 1150
FauI	CCCGC	1 cut(s)	373
FbaI	TGATCA	1 cut(s)	351
Fnu4HI	GCNGC	3 cut(s)	306, 1117, 1373
FokI	GGATG	2 cut(s)	1079, 1231
Fsp4HI	GCNGC	3 cut(s)	306, 1117, 1373
FspBI	CTAG	2 cut(s)	32, 765
GlaI	GCGC	1 cut(s)	1107
GluI	GCNGC	3 cut(s)	306, 1117, 1373
HaeIII	GGCC	1 cut(s)	533
HapII	CCGG	8 cut(s)	147, 377, 382, 517, 534, 593, 701, 806
HgaI	GACGC	3 cut(s)	227, 265, 1295
HhaI	GCGC	1 cut(s)	1108
Hin1I	GRCGYC	1 cut(s)	219
Hin1II	CATG	5 cut(s)	352, 892, 997, 1006, 1154
Hin6I	GCGC	1 cut(s)	1106
HinP1I	GCGC	1 cut(s)	1106
HincII	GTYRAC	1 cut(s)	322
HindII	GTYRAC	1 cut(s)	322
HindIII	AAGCTT	1 cut(s)	1223
HinfI	GANTC	1 cut(s)	326
HpaII	CCGG	8 cut(s)	147, 377, 382, 517, 534, 593, 701, 806
HphI	GGTGA	5 cut(s)	112, 190, 440, 490, 1036
Hpy188I	TCNGA	6 cut(s)	53, 111, 217, 432, 936, 1059
Hpy188III	TCNNGA	2 cut(s)	266, 639
HpyAV	CCTTC	5 cut(s)	72, 772, 877, 1054, 1161
HpyCH4III	ACNGT	4 cut(s)	319, 421, 655, 1366
HpyCH4IV	ACGT	3 cut(s)	25, 540, 757
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1076
HpyF3I	CTNAG	4 cut(s)	19, 108, 214, 851
HpySE526I	ACGT	3 cut(s)	25, 540, 757
Hsp92I	GRCGYC	1 cut(s)	219
Hsp92II	CATG	5 cut(s)	352, 892, 997, 1006, 1154
HspAI	GCGC	1 cut(s)	1106
KpnI	GGTACC	1 cut(s)	593
Ksp22I	TGATCA	1 cut(s)	351
Kzo9I	GATC	5 cut(s)	268, 351, 427, 913, 990
LmnI	GCTCC	1 cut(s)	907
Lsp1109I	GCAGC	2 cut(s)	1128, 1384
LweI	GCATC	2 cut(s)	1057, 1089
MaeI	CTAG	2 cut(s)	32, 765
MaeII	ACGT	3 cut(s)	25, 540, 757
MaeIII	GTNAC	5 cut(s)	496, 519, 709, 919, 1024
MalI	GATC	5 cut(s)	270, 353, 429, 915, 992
MboI	GATC	5 cut(s)	268, 351, 427, 913, 990
MboII	GAAGA	3 cut(s)	223, 331, 988
MhlI	GDGCHC	1 cut(s)	1133
MluCI	AATT	4 cut(s)	142, 610, 751, 1190
MlyI	GAGTC	1 cut(s)	320
MmeI	TCCRAC	4 cut(s)	187, 193, 1037, 1071
Mph1103I	ATGCAT	1 cut(s)	1072
MroXI	GAANNNNTTC	1 cut(s)	194
MseI	TTAA	5 cut(s)	657, 801, 1328, 1368, 1377
MspCI	CTTAAG	1 cut(s)	1376
MspI	CCGG	8 cut(s)	147, 377, 382, 517, 534, 593, 701, 806
Mva1269I	GAATGC	1 cut(s)	368
MvnI	CGCG	3 cut(s)	276, 587, 640
MwoI	GCNNNNNNNGC	1 cut(s)	1076
NdeII	GATC	5 cut(s)	268, 351, 427, 913, 990
NheI	GCTAGC	1 cut(s)	764
NlaIII	CATG	5 cut(s)	352, 892, 997, 1006, 1154
NlaIV	GGNNCC	2 cut(s)	591, 909
NmuCI	GTSAC	3 cut(s)	496, 519, 1024
NruI	TCGCGA	1 cut(s)	640
NsiI	ATGCAT	1 cut(s)	1072
NspI	RCATGY	1 cut(s)	892
PcsI	WCGNNNNNNNCGW	1 cut(s)	273
PctI	GAATGC	1 cut(s)	368
PdmI	GAANNNNTTC	1 cut(s)	194
PinAI	ACCGGT	1 cut(s)	381
PkrI	GCNGC	3 cut(s)	307, 1118, 1374
PleI	GAGTC	1 cut(s)	320
PmaCI	CACGTG	1 cut(s)	541
PmlI	CACGTG	1 cut(s)	541
PpsI	GAGTC	1 cut(s)	320
Ppu21I	YACGTR	2 cut(s)	541, 758
PspCI	CACGTG	1 cut(s)	541
PspEI	GGTNACC	1 cut(s)	496
PspN4I	GGNNCC	2 cut(s)	591, 909
PspPI	GGNCC	2 cut(s)	379, 384
RruI	TCGCGA	1 cut(s)	640
RsaI	GTAC	6 cut(s)	24, 423, 591, 652, 1246, 1363
RsaNI	GTAC	6 cut(s)	23, 422, 590, 651, 1245, 1362
Rsr2I	CGGWCCG	1 cut(s)	379
RsrII	CGGWCCG	1 cut(s)	379
SaqAI	TTAA	5 cut(s)	657, 801, 1328, 1368, 1377
SatI	GCNGC	3 cut(s)	306, 1117, 1373
Sau3AI	GATC	5 cut(s)	268, 351, 427, 913, 990
Sau96I	GGNCC	2 cut(s)	379, 384
ScaI	AGTACT	1 cut(s)	652
SchI	GAGTC	1 cut(s)	320
SduI	GDGCHC	1 cut(s)	1133
SfaNI	GCATC	2 cut(s)	1057, 1089
SinI	GGWCC	2 cut(s)	379, 384
SmlI	CTYRAG	1 cut(s)	1376
SmoI	CTYRAG	1 cut(s)	1376
SpeI	ACTAGT	1 cut(s)	31
Sse9I	AATT	4 cut(s)	142, 610, 751, 1190
SsiI	CCGC	6 cut(s)	249, 274, 306, 366, 587, 988
SspMI	CTAG	2 cut(s)	32, 765
TaaI	ACNGT	4 cut(s)	319, 421, 655, 1366
TaiI	ACGT	3 cut(s)	28, 543, 760
TaqI	TCGA	3 cut(s)	267, 690, 950
TasI	AATT	4 cut(s)	142, 610, 751, 1190
TatI	WGTACW	3 cut(s)	650, 1244, 1361
TauI	GCSGC	1 cut(s)	308
Tru1I	TTAA	5 cut(s)	657, 801, 1328, 1368, 1377
Tru9I	TTAA	5 cut(s)	657, 801, 1328, 1368, 1377
TscAI	CASTG	2 cut(s)	558, 1086
TseFI	GTSAC	3 cut(s)	496, 519, 1024
TseI	GCWGC	2 cut(s)	1116, 1372
Tsp45I	GTSAC	3 cut(s)	496, 519, 1024
TspDTI	ATGAA	7 cut(s)	578, 848, 1026, 1058, 1139, 1220, 1235
TspRI	CASTG	2 cut(s)	558, 1086
Vha464I	CTTAAG	1 cut(s)	1376
VneI	GTGCAC	1 cut(s)	1129
VpaK11BI	GGWCC	2 cut(s)	379, 384
XapI	RAATTY	2 cut(s)	142, 751
XceI	RCATGY	1 cut(s)	892
XmnI	GAANNNNTTC	1 cut(s)	194
XspI	CTAG	2 cut(s)	32, 765
ZrmI	AGTACT	1 cut(s)	652
Zsp2I	ATGCAT	1 cut(s)	1072

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.