MD15G1324900.v1.1

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002083
Physical Location & Seq
Forward (+)
34629958 .. 34630701
744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1324900.v1.1.491

Sequence Viewer

Length: 744 bp
ATGAGGGTCACTGATGGTCCTGATTTCGGAGCCAACTCCCTCCTCAACCTACCAGCCGTTAAAAACATGTCGGTGCTGACCGTGGAGAGGCATCCTTGGCAAGGGTCAAACCAACACGGCTTGCCTTACCCTTCCTATTTCCACCCGTCCACTTGGCAGGAGATGGTGGCGTGGCAGAACAGAGTGAGGGGAATGGACCGACCCCACTTGTTTTCTTTCATCGGTGGGCCCAGAAAGGGGTTAGAAAAAGCGGCCGTCCGGGACGAGTTCATTAGGCAATGCGGTGAGTCGACCCGCTGCATGCTTTTAAAATGTGGGTGTGGGGCCGGCAAGTGCCACGAGCCCAGCGAGGTGCTAAAGGTGATGTCCGAGTCGCAGTTCTGCTTACAAGCGCCCGGTGACTCGTTCACCCGGCGGTCGACGTTTGACTCGGTGCTCGCCGGCTGCATACCGGTTTTCTCGTCGCCGCACACGGCGTACACTCAGTACAAGTGGTTTTTACCTGGTGACGTGAGCACGTACTCGGTTTACATTGACGAGAAGAGCGACGCGAGTAAAAGGATCGAAGAGGAGCTGCTGAAGTTTCCGAACGAAAAAGTGACGGCGATGCGGGAAATGCTCATAGAATTGATCCCAAGTCTGACTTACGCGCATCCGAATGCGACTAATCTTGGGTTTGGGGACGCCGTGGACGTTGCACTTGCATCGTTGGCCAAGCACATCCAGAAAATATACGATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

248

Amino Acids

27.67

Weight (kDa)

7.07

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 9 - 182 2.6e-36 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 290, 419
AccII CGCG 2 cut(s) 551, 650
AciI CCGC 6 cut(s) 251, 282, 295, 415, 467, 610
AclWI GGATC 2 cut(s) 569, 625
AcoI YGGCCR 2 cut(s) 252, 711
AcuI CTGAAG 1 cut(s) 599
AcyI GRCGYC 1 cut(s) 684
AfaI GTAC 3 cut(s) 479, 488, 521
AfiI CCNNNNNNNGG 5 cut(s) 26, 87, 101, 236, 237
AflIII ACRYGT 1 cut(s) 66
AgeI ACCGGT 1 cut(s) 451
AjiI CACGTC 1 cut(s) 511
AjnI CCWGG 1 cut(s) 502
AluBI AGCT 1 cut(s) 574
AluI AGCT 1 cut(s) 574
Alw21I GWGCWC 2 cut(s) 438, 518
AlwI GGATC 2 cut(s) 569, 625
AoxI GGCC 4 cut(s) 227, 252, 324, 711
ApaI GGGCCC 1 cut(s) 231
ApeKI GCWGC 3 cut(s) 297, 444, 574
AsiGI ACCGGT 1 cut(s) 451
AspLEI GCGC 2 cut(s) 394, 652
AspS9I GGNCC 5 cut(s) 17, 196, 227, 228, 324
AsuC2I CCSGG 3 cut(s) 260, 396, 412
AsuHPI GGTGA 5 cut(s) 296, 373, 400, 410, 518
AvaII GGWCC 2 cut(s) 17, 196
BaeGI GKGCMC 1 cut(s) 231
BalI TGGCCA 1 cut(s) 713
BanII GRGCYC 2 cut(s) 231, 345
BauI CACGAG 1 cut(s) 338
Bbv12I GWGCWC 2 cut(s) 438, 518
BbvI GCAGC 3 cut(s) 284, 431, 561
BccI CCATC 2 cut(s) 8, 157
BceAI ACGGC 6 cut(s) 41, 133, 239, 489, 618, 671
BcgI CGANNNNNNTGC 2 cut(s) 687, 721
BciT130I CCWGG 1 cut(s) 504
BcnI CCSGG 3 cut(s) 260, 396, 412
BfoI RGCGCY 1 cut(s) 395
BisI GCNGC 5 cut(s) 252, 298, 445, 467, 575
BlsI GCNGC 5 cut(s) 253, 299, 446, 468, 576
Bme1390I CCNGG 4 cut(s) 260, 396, 412, 504
Bme18I GGWCC 2 cut(s) 17, 196
BmgBI CACGTC 1 cut(s) 511
BmgT120I GGNCC 5 cut(s) 17, 196, 227, 228, 324
BmiI GGNNCC 3 cut(s) 31, 229, 325
BmrFI CCNGG 4 cut(s) 260, 396, 412, 504
BmsI GCATC 4 cut(s) 100, 597, 661, 713
BpuMI CCSGG 3 cut(s) 260, 396, 412
BsaAI YACGTR 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 684
BsaJI CCNNGG 3 cut(s) 81, 95, 687
BsaWI WCCGGW 1 cut(s) 451
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 87, 101, 236, 237
Bse118I RCCGGY 3 cut(s) 326, 440, 451
Bse3DI GCAATG 1 cut(s) 284
BseBI CCWGG 1 cut(s) 504
BseDI CCNNGG 3 cut(s) 81, 95, 687
BseGI GGATG 3 cut(s) 91, 652, 720
BseLI CCNNNNNNNGG 5 cut(s) 26, 87, 101, 236, 237
BseMI GCAATG 1 cut(s) 284
BseMII CTCAG 1 cut(s) 497
BseRI GAGGAG 2 cut(s) 32, 584
BseSI GKGCMC 1 cut(s) 231
BseX3I CGGCCG 1 cut(s) 252
BseXI GCAGC 3 cut(s) 284, 431, 561
BseYI CCCAGC 1 cut(s) 344
Bsh1236I CGCG 2 cut(s) 551, 650
Bsh1285I CGRYCG 2 cut(s) 255, 419
BshFI GGCC 4 cut(s) 229, 254, 326, 713
BshTI ACCGGT 1 cut(s) 451
BsiEI CGRYCG 2 cut(s) 255, 419
BsiHKAI GWGCWC 2 cut(s) 438, 518
BsiSI CCGG 6 cut(s) 259, 327, 396, 412, 441, 452
BslFI GGGAC 2 cut(s) 275, 695
BslI CCNNNNNNNGG 5 cut(s) 26, 87, 101, 236, 237
BsmFI GGGAC 2 cut(s) 275, 695
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 4 cut(s) 229, 254, 326, 713
Bsp120I GGGCCC 1 cut(s) 227
Bsp1286I GDGCHC 4 cut(s) 231, 345, 438, 518
Bsp143I GATC 2 cut(s) 561, 630
BspACI CCGC 6 cut(s) 251, 282, 295, 415, 467, 610
BspANI GGCC 4 cut(s) 229, 254, 326, 713
BspCNI CTCAG 1 cut(s) 496
BspFNI CGCG 2 cut(s) 551, 650
BspLI GGNNCC 3 cut(s) 31, 229, 325
BspPI GGATC 2 cut(s) 569, 625
BspQI GCTCTTC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 284
BsrFI RCCGGY 3 cut(s) 326, 440, 451
BssAI RCCGGY 3 cut(s) 326, 440, 451
BssECI CCNNGG 3 cut(s) 81, 95, 687
BssMI GATC 2 cut(s) 561, 630
BssNI GRCGYC 1 cut(s) 684
BssSI CACGAG 1 cut(s) 338
BssT1I CCWWGG 1 cut(s) 95
Bst2BI CACGAG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 2 cut(s) 536, 561
BstACI GRCGYC 1 cut(s) 684
BstBAI YACGTR 1 cut(s) 519
BstC8I GCNNGC 5 cut(s) 122, 302, 328, 438, 442
BstDEI CTNAG 1 cut(s) 483
BstDSI CCRYGG 2 cut(s) 81, 687
BstENI CCTNNNNNAGG 1 cut(s) 99
BstF5I GGATG 3 cut(s) 91, 652, 720
BstFNI CGCG 2 cut(s) 551, 650
BstH2I RGCGCY 1 cut(s) 395
BstHHI GCGC 2 cut(s) 394, 652
BstKTI GATC 2 cut(s) 564, 633
BstMBI GATC 2 cut(s) 561, 630
BstMCI CGRYCG 2 cut(s) 255, 419
BstMWI GCNNNNNNNGC 3 cut(s) 97, 616, 710
BstNI CCWGG 1 cut(s) 504
BstNSI RCATGY 2 cut(s) 70, 304
BstSCI CCNGG 4 cut(s) 258, 394, 410, 502
BstSLI GKGCMC 1 cut(s) 231
BstUI CGCG 2 cut(s) 551, 650
BstV1I GCAGC 3 cut(s) 284, 431, 561
BstZI CGGCCG 1 cut(s) 252
BsuRI GGCC 4 cut(s) 229, 254, 326, 713
BtgI CCRYGG 2 cut(s) 81, 687
BtgZI GCGATG 1 cut(s) 620
BtrI CACGTC 1 cut(s) 511
BtsCI GGATG 3 cut(s) 91, 652, 720
BtsIMutI CAGTG 1 cut(s) 9
Cac8I GCNNGC 5 cut(s) 122, 302, 328, 438, 442
CfoI GCGC 2 cut(s) 394, 652
Cfr10I RCCGGY 3 cut(s) 326, 440, 451
Cfr13I GGNCC 5 cut(s) 17, 196, 227, 228, 324
CseI GACGC 2 cut(s) 557, 692
CsiI ACCWGGT 1 cut(s) 502
Csp6I GTAC 3 cut(s) 478, 487, 520
CspAI ACCGGT 1 cut(s) 451
CviAII CATG 2 cut(s) 67, 301
CviQI GTAC 3 cut(s) 478, 487, 520
DdeI CTNAG 1 cut(s) 483
DpnI GATC 2 cut(s) 563, 632
DpnII GATC 2 cut(s) 561, 630
DraI TTTAAA 1 cut(s) 309
EaeI YGGCCR 2 cut(s) 252, 711
EagI CGGCCG 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 536, 561
EarI CTCTTC 2 cut(s) 536, 561
EclXI CGGCCG 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 95
Eco24I GRGCYC 2 cut(s) 231, 345
Eco47I GGWCC 2 cut(s) 17, 196
Eco52I CGGCCG 1 cut(s) 252
Eco57I CTGAAG 1 cut(s) 599
EcoNI CCTNNNNNAGG 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 502
EcoT14I CCWWGG 1 cut(s) 95
EcoT38I GRGCYC 2 cut(s) 231, 345
ErhI CCWWGG 1 cut(s) 95
FaeI CATG 2 cut(s) 70, 304
FaiI YATR 5 cut(s) 68, 302, 449, 623, 733
FalI AAGNNNNNCTT 2 cut(s) 628, 660
FaqI GGGAC 2 cut(s) 275, 695
FatI CATG 2 cut(s) 66, 300
FauI CCCGC 2 cut(s) 302, 603
FblI GTMKAC 2 cut(s) 290, 419
Fnu4HI GCNGC 5 cut(s) 252, 298, 445, 467, 575
FokI GGATG 3 cut(s) 78, 639, 707
FriOI GRGCYC 2 cut(s) 231, 345
Fsp4HI GCNGC 5 cut(s) 252, 298, 445, 467, 575
GlaI GCGC 2 cut(s) 393, 651
GluI GCNGC 5 cut(s) 252, 298, 445, 467, 575
GsaI CCCAGC 1 cut(s) 348
HaeII RGCGCY 1 cut(s) 395
HaeIII GGCC 4 cut(s) 229, 254, 326, 713
HapII CCGG 6 cut(s) 259, 327, 396, 412, 441, 452
HgaI GACGC 2 cut(s) 557, 692
HhaI GCGC 2 cut(s) 394, 652
Hin1I GRCGYC 1 cut(s) 684
Hin1II CATG 2 cut(s) 70, 304
Hin6I GCGC 2 cut(s) 392, 650
HinP1I GCGC 2 cut(s) 392, 650
HincII GTYRAC 2 cut(s) 291, 420
HindII GTYRAC 2 cut(s) 291, 420
HinfI GANTC 4 cut(s) 287, 371, 401, 428
HpaII CCGG 6 cut(s) 259, 327, 396, 412, 441, 452
HphI GGTGA 5 cut(s) 296, 373, 400, 410, 518
Hpy166II GTNNAC 7 cut(s) 150, 291, 408, 420, 480, 529, 691
Hpy188I TCNGA 5 cut(s) 29, 370, 588, 642, 657
Hpy188III TCNNGA 2 cut(s) 20, 724
Hpy8I GTNNAC 7 cut(s) 150, 291, 408, 420, 480, 529, 691
Hpy99I CGWCG 3 cut(s) 424, 466, 551
HpyAV CCTTC 1 cut(s) 141
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 4 cut(s) 422, 510, 518, 693
HpyCH4V TGCA 4 cut(s) 300, 447, 698, 704
HpyF10VI GCNNNNNNNGC 3 cut(s) 97, 616, 710
HpyF3I CTNAG 1 cut(s) 483
HpySE526I ACGT 4 cut(s) 422, 510, 518, 693
Hsp92I GRCGYC 1 cut(s) 684
Hsp92II CATG 2 cut(s) 70, 304
HspAI GCGC 2 cut(s) 392, 650
KroI GCCGGC 2 cut(s) 326, 440
KroNI GCCGGC 2 cut(s) 328, 442
Kzo9I GATC 2 cut(s) 561, 630
LguI GCTCTTC 1 cut(s) 536
LmnI GCTCC 2 cut(s) 29, 571
Lsp1109I GCAGC 3 cut(s) 284, 431, 561
LweI GCATC 4 cut(s) 100, 597, 661, 713
MabI ACCWGGT 1 cut(s) 502
MaeII ACGT 4 cut(s) 422, 510, 518, 693
MaeIII GTNAC 4 cut(s) 7, 398, 506, 598
MalI GATC 2 cut(s) 563, 632
MboI GATC 2 cut(s) 561, 630
MboII GAAGA 2 cut(s) 553, 578
MhlI GDGCHC 4 cut(s) 231, 345, 438, 518
MlsI TGGCCA 1 cut(s) 713
MluCI AATT 1 cut(s) 626
MluNI TGGCCA 1 cut(s) 713
MlyI GAGTC 4 cut(s) 296, 380, 395, 422
MnlI CCTC 6 cut(s) 50, 53, 81, 180, 343, 562
Mox20I TGGCCA 1 cut(s) 713
MroNI GCCGGC 2 cut(s) 326, 440
MscI TGGCCA 1 cut(s) 713
MseI TTAA 2 cut(s) 60, 308
MslI CAYNNNNRTG 2 cut(s) 71, 657
Msp20I TGGCCA 1 cut(s) 713
MspA1I CMGCKG 1 cut(s) 297
MspI CCGG 6 cut(s) 259, 327, 396, 412, 441, 452
MspR9I CCNGG 4 cut(s) 260, 396, 412, 504
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 1 cut(s) 504
MvnI CGCG 2 cut(s) 551, 650
MwoI GCNNNNNNNGC 3 cut(s) 97, 616, 710
NaeI GCCGGC 2 cut(s) 328, 442
NciI CCSGG 3 cut(s) 260, 396, 412
NdeII GATC 2 cut(s) 561, 630
NgoMIV GCCGGC 2 cut(s) 326, 440
NlaIII CATG 2 cut(s) 70, 304
NlaIV GGNNCC 3 cut(s) 31, 229, 325
NmuCI GTSAC 4 cut(s) 7, 398, 506, 598
NspI RCATGY 2 cut(s) 70, 304
PaeI GCATGC 1 cut(s) 304
PciI ACATGT 1 cut(s) 66
PciSI GCTCTTC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 3 cut(s) 345, 543, 690
PctI GAATGC 1 cut(s) 664
PdiI GCCGGC 2 cut(s) 328, 442
PfoI TCCNGGA 1 cut(s) 258
PinAI ACCGGT 1 cut(s) 451
PkrI GCNGC 5 cut(s) 253, 299, 446, 468, 576
PleI GAGTC 4 cut(s) 295, 379, 395, 422
PpsI GAGTC 4 cut(s) 295, 379, 395, 422
Ppu21I YACGTR 1 cut(s) 519
PscI ACATGT 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 502
PspFI CCCAGC 1 cut(s) 344
PspGI CCWGG 1 cut(s) 502
PspN4I GGNNCC 3 cut(s) 31, 229, 325
PspOMI GGGCCC 1 cut(s) 227
PspPI GGNCC 5 cut(s) 17, 196, 227, 228, 324
RsaI GTAC 3 cut(s) 479, 488, 521
RsaNI GTAC 3 cut(s) 478, 487, 520
RseI CAYNNNNRTG 2 cut(s) 71, 657
SalI GTCGAC 2 cut(s) 289, 418
SapI GCTCTTC 1 cut(s) 536
SaqAI TTAA 2 cut(s) 60, 308
SatI GCNGC 5 cut(s) 252, 298, 445, 467, 575
Sau3AI GATC 2 cut(s) 561, 630
Sau96I GGNCC 5 cut(s) 17, 196, 227, 228, 324
SchI GAGTC 4 cut(s) 296, 380, 395, 422
ScrFI CCNGG 4 cut(s) 260, 396, 412, 504
SduI GDGCHC 4 cut(s) 231, 345, 438, 518
SetI ASST 9 cut(s) 51, 354, 363, 425, 505, 513, 521, 576, 696
SexAI ACCWGGT 1 cut(s) 502
SfaNI GCATC 4 cut(s) 100, 597, 661, 713
SinI GGWCC 2 cut(s) 17, 196
SmiMI CAYNNNNRTG 2 cut(s) 71, 657
SphI GCATGC 1 cut(s) 304
Sse9I AATT 1 cut(s) 626
SsiI CCGC 6 cut(s) 251, 282, 295, 415, 467, 610
StyD4I CCNGG 4 cut(s) 258, 394, 410, 502
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 4 cut(s) 425, 513, 521, 696
TaqI TCGA 3 cut(s) 290, 419, 564
TaqII GACCGA 1 cut(s) 213
TasI AATT 1 cut(s) 626
TatI WGTACW 1 cut(s) 486
TauI GCSGC 2 cut(s) 254, 469
Tru1I TTAA 2 cut(s) 60, 308
Tru9I TTAA 2 cut(s) 60, 308
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 4 cut(s) 7, 398, 506, 598
TseI GCWGC 3 cut(s) 297, 444, 574
Tsp45I GTSAC 4 cut(s) 7, 398, 506, 598
TspDTI ATGAA 2 cut(s) 208, 259
TspRI CASTG 1 cut(s) 16
VpaK11BI GGWCC 2 cut(s) 17, 196
XagI CCTNNNNNAGG 1 cut(s) 99
XceI RCATGY 2 cut(s) 70, 304
XmiI GTMKAC 2 cut(s) 290, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.