Rroxscaffold_2G00130190

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002083
Physical Location & Seq
Forward (+)
66297169 .. 66298164
996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00130190.1

Sequence Viewer

Length: 879 bp
ATGCAATTCGCCTCAGAGCTCATCTTCCATGCACGTTTGCTCAACCATCCTTGCCGCACGTGGGATCATGCAAGTGCCAATTTATTTTATGTCCCCTTTTACGGCAGCCTCTACTCTCTGAGCCATTTCAACGAGTCCAACTCCACCATACGCAACCAGCTCGCGGTGGACTTGGCCGAGTACATTCAGGCGCAACCCATGTGGAAGAGGAACAATGGTAAGGACCACTTTATGGTCCAAGGGAGAGTCGCATTCAATTACATGGGGATCGACCAATTTGATAAAGCCAACATCCTTCCAGCGAAAAACATGTCTGTGTTCATCATAGAGAGATCTAAGTCCAGCGAGCACCAACATGGCATACCCTACCGTTCGTATTTTGACCCCTCTACGTCGGAACAAATAGTGACATGGCAAAACAAGGTGAGGGAAATGGAGAGGCCTCACTTGTTTTCCTTTGTACTAGGTGGGCCAAGAAAGAACAAGGGGTTGCGAGAGGAGTGCATAAGGCAATGTGGTGAGTCAAATCGTAGCAAGCTTTTAAATTGCGGCGGATCCGGGGACGGTAAAAAGTGCCTGCAACAGGTACGCATGGTACTAAAGAGAATTGAATCAGTACTAAACATTAATCAGTACGCATGGTTTTTACCTGAAGATGCGAGCGCGTACTCTATGTTCATAGATCAGAAGAGTAATGCGAGAAAGAGAATTGAAGACCAGTTGTTGAAGATTTCGAGCGACAGGGTTGAGAGGATGAGTGAAAATCTTATAGACTTGATTCCGAGCTTGACTTATGCCCACCCAAATGCGTCAAGTTTAGGGGTTGAGGATGTTGCACTTGCATCATTGGCCAAGCATGTCAACAGAATTATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

292

Amino Acids

33.53

Weight (kDa)

9.02

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 2 - 181 1.2e-29 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 872
AccB7I CCANNNNNTGG 1 cut(s) 232
AccII CGCG 2 cut(s) 164, 665
AciI CCGC 4 cut(s) 55, 164, 549, 552
AclWI GGATC 4 cut(s) 72, 275, 549, 562
AcoI YGGCCR 2 cut(s) 174, 849
AcuI CTGAAG 1 cut(s) 672
AcvI CACGTG 1 cut(s) 60
AfaI GTAC 7 cut(s) 182, 462, 588, 597, 618, 635, 668
AfiI CCNNNNNNNGG 5 cut(s) 61, 101, 163, 232, 583
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 5 cut(s) 130, 256, 611, 713, 727
AluBI AGCT 4 cut(s) 19, 160, 538, 786
AluI AGCT 4 cut(s) 19, 160, 538, 786
Alw21I GWGCWC 2 cut(s) 21, 351
AlwI GGATC 4 cut(s) 72, 275, 549, 562
AoxI GGCC 4 cut(s) 174, 440, 470, 849
ApeKI GCWGC 1 cut(s) 105
ArsI GACNNNNNNTTYG 2 cut(s) 296, 328
AseI ATTAAT 1 cut(s) 627
AspLEI GCGC 2 cut(s) 193, 665
AspS9I GGNCC 3 cut(s) 223, 235, 470
AsuC2I CCSGG 1 cut(s) 559
AsuHPI GGTGA 2 cut(s) 436, 530
AvaII GGWCC 2 cut(s) 223, 235
BalI TGGCCA 1 cut(s) 851
BamHI GGATCC 1 cut(s) 554
BanII GRGCYC 1 cut(s) 21
BbrPI CACGTG 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 720
Bbv12I GWGCWC 2 cut(s) 21, 351
BbvI GCAGC 1 cut(s) 117
BccI CCATC 1 cut(s) 54
BceAI ACGGC 1 cut(s) 118
BcgI CGANNNNNNTGC 4 cut(s) 142, 176, 483, 517
BcnI CCSGG 1 cut(s) 559
BfaI CTAG 1 cut(s) 464
BglII AGATCT 1 cut(s) 332
BisI GCNGC 3 cut(s) 55, 106, 550
BlsI GCNGC 3 cut(s) 56, 107, 551
BmcAI AGTACT 1 cut(s) 618
Bme1390I CCNGG 1 cut(s) 559
Bme18I GGWCC 2 cut(s) 223, 235
BmgT120I GGNCC 3 cut(s) 223, 235, 470
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 1 cut(s) 559
BmsI GCATC 2 cut(s) 646, 851
BpiI GAAGAC 1 cut(s) 720
BplI GAGNNNNNCTC 2 cut(s) 125, 157
BpuMI CCSGG 1 cut(s) 559
BsaAI YACGTR 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 238, 558
Bsc4I CCNNNNNNNGG 5 cut(s) 61, 101, 163, 232, 583
Bse1I ACTGG 1 cut(s) 718
Bse3DI GCAATG 1 cut(s) 518
BseDI CCNNGG 2 cut(s) 238, 558
BseGI GGATG 4 cut(s) 46, 291, 759, 835
BseLI CCNNNNNNNGG 5 cut(s) 61, 101, 163, 232, 583
BseMI GCAATG 1 cut(s) 518
BseMII CTCAG 2 cut(s) 27, 110
BseNI ACTGG 1 cut(s) 718
BseRI GAGGAG 1 cut(s) 512
BseXI GCAGC 1 cut(s) 117
Bsh1236I CGCG 2 cut(s) 164, 665
BshFI GGCC 4 cut(s) 176, 442, 472, 851
BsiHKAI GWGCWC 2 cut(s) 21, 351
BsiSI CCGG 1 cut(s) 558
BslFI GGGAC 2 cut(s) 77, 575
BslI CCNNNNNNNGG 5 cut(s) 61, 101, 163, 232, 583
BsmFI GGGAC 2 cut(s) 77, 575
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 4 cut(s) 176, 442, 472, 851
Bsp1286I GDGCHC 2 cut(s) 21, 351
Bsp143I GATC 5 cut(s) 64, 267, 332, 554, 682
BspACI CCGC 4 cut(s) 55, 164, 549, 552
BspANI GGCC 4 cut(s) 176, 442, 472, 851
BspCNI CTCAG 2 cut(s) 26, 111
BspFNI CGCG 2 cut(s) 164, 665
BspLI GGNNCC 1 cut(s) 556
BspPI GGATC 4 cut(s) 72, 275, 549, 562
BsrDI GCAATG 1 cut(s) 518
BsrI ACTGG 1 cut(s) 718
BssECI CCNNGG 2 cut(s) 238, 558
BssMI GATC 5 cut(s) 64, 267, 332, 554, 682
BssT1I CCWWGG 1 cut(s) 238
Bst4CI ACNGT 2 cut(s) 371, 566
Bst6I CTCTTC 2 cut(s) 200, 683
BstBAI YACGTR 1 cut(s) 60
BstC8I GCNNGC 5 cut(s) 162, 347, 536, 578, 661
BstDEI CTNAG 3 cut(s) 13, 119, 336
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 4 cut(s) 46, 291, 759, 835
BstFNI CGCG 2 cut(s) 164, 665
BstHHI GCGC 2 cut(s) 193, 665
BstKTI GATC 5 cut(s) 67, 270, 335, 557, 685
BstMBI GATC 5 cut(s) 64, 267, 332, 554, 682
BstMWI GCNNNNNNNGC 1 cut(s) 848
BstNSI RCATGY 2 cut(s) 313, 860
BstSCI CCNGG 1 cut(s) 557
BstUI CGCG 2 cut(s) 164, 665
BstV1I GCAGC 1 cut(s) 117
BstV2I GAAGAC 1 cut(s) 720
BstX2I RGATCY 2 cut(s) 332, 554
BstYI RGATCY 2 cut(s) 332, 554
BsuRI GGCC 4 cut(s) 176, 442, 472, 851
BtsCI GGATG 4 cut(s) 46, 291, 759, 835
Cac8I GCNNGC 5 cut(s) 162, 347, 536, 578, 661
CfoI GCGC 2 cut(s) 193, 665
Cfr13I GGNCC 3 cut(s) 223, 235, 470
CseI GACGC 1 cut(s) 798
Csp6I GTAC 7 cut(s) 181, 461, 587, 596, 617, 634, 667
CspCI CAANNNNNGTGG 2 cut(s) 182, 217
CviQI GTAC 7 cut(s) 181, 461, 587, 596, 617, 634, 667
DdeI CTNAG 3 cut(s) 13, 119, 336
DpnI GATC 5 cut(s) 66, 269, 334, 556, 684
DpnII GATC 5 cut(s) 64, 267, 332, 554, 682
DraI TTTAAA 1 cut(s) 543
EaeI YGGCCR 2 cut(s) 174, 849
Eam1104I CTCTTC 2 cut(s) 200, 683
EarI CTCTTC 2 cut(s) 200, 683
EciI GGCGGA 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 238
Eco147I AGGCCT 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 223, 235
Eco53kI GAGCTC 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 672
Eco72I CACGTG 1 cut(s) 60
EcoICRI GAGCTC 1 cut(s) 19
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoT14I CCWWGG 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 238
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FaqI GGGAC 2 cut(s) 77, 575
Fnu4HI GCNGC 3 cut(s) 55, 106, 550
FokI GGATG 4 cut(s) 33, 278, 766, 842
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 3 cut(s) 55, 106, 550
FspBI CTAG 1 cut(s) 464
GlaI GCGC 2 cut(s) 192, 664
GluI GCNGC 3 cut(s) 55, 106, 550
HaeIII GGCC 4 cut(s) 176, 442, 472, 851
HapII CCGG 1 cut(s) 558
HgaI GACGC 1 cut(s) 798
HhaI GCGC 2 cut(s) 193, 665
Hin6I GCGC 2 cut(s) 191, 663
HinP1I GCGC 2 cut(s) 191, 663
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 5 cut(s) 134, 246, 521, 611, 778
HpaII CCGG 1 cut(s) 558
HphI GGTGA 2 cut(s) 436, 530
Hpy166II GTNNAC 2 cut(s) 169, 862
Hpy188I TCNGA 5 cut(s) 16, 120, 397, 687, 783
Hpy8I GTNNAC 2 cut(s) 169, 862
Hpy99I CGWCG 1 cut(s) 397
HpyAV CCTTC 1 cut(s) 305
HpyCH4III ACNGT 2 cut(s) 371, 566
HpyCH4IV ACGT 3 cut(s) 34, 59, 392
HpyCH4V TGCA 7 cut(s) 4, 32, 71, 504, 580, 836, 842
HpyF10VI GCNNNNNNNGC 1 cut(s) 848
HpyF3I CTNAG 3 cut(s) 13, 119, 336
HpySE526I ACGT 3 cut(s) 34, 59, 392
HspAI GCGC 2 cut(s) 191, 663
Kzo9I GATC 5 cut(s) 64, 267, 332, 554, 682
Lsp1109I GCAGC 1 cut(s) 117
LweI GCATC 2 cut(s) 646, 851
MaeI CTAG 1 cut(s) 464
MaeII ACGT 3 cut(s) 34, 59, 392
MaeIII GTNAC 1 cut(s) 406
MalI GATC 5 cut(s) 66, 269, 334, 556, 684
MboI GATC 5 cut(s) 64, 267, 332, 554, 682
MboII GAAGA 6 cut(s) 16, 217, 665, 700, 725, 739
MflI RGATCY 2 cut(s) 332, 554
MhlI GDGCHC 2 cut(s) 21, 351
MlsI TGGCCA 1 cut(s) 851
MluCI AATT 9 cut(s) 5, 79, 256, 275, 544, 606, 708, 867, 874
MluNI TGGCCA 1 cut(s) 851
MlyI GAGTC 3 cut(s) 143, 255, 530
MmeI TCCRAC 2 cut(s) 162, 375
Mox20I TGGCCA 1 cut(s) 851
MscI TGGCCA 1 cut(s) 851
MseI TTAA 3 cut(s) 542, 627, 877
MslI CAYNNNNRTG 4 cut(s) 72, 314, 354, 804
Msp20I TGGCCA 1 cut(s) 851
MspI CCGG 1 cut(s) 558
MspR9I CCNGG 1 cut(s) 559
Mva1269I GAATGC 1 cut(s) 251
MvnI CGCG 2 cut(s) 164, 665
MwoI GCNNNNNNNGC 1 cut(s) 848
NciI CCSGG 1 cut(s) 559
NdeII GATC 5 cut(s) 64, 267, 332, 554, 682
NlaIV GGNNCC 1 cut(s) 556
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 406
NspI RCATGY 2 cut(s) 313, 860
PceI AGGCCT 1 cut(s) 442
PciI ACATGT 1 cut(s) 309
PctI GAATGC 1 cut(s) 251
PfeI GAWTC 2 cut(s) 611, 778
PflMI CCANNNNNTGG 1 cut(s) 232
PkrI GCNGC 3 cut(s) 56, 107, 551
PleI GAGTC 3 cut(s) 142, 254, 529
PmaCI CACGTG 1 cut(s) 60
PmlI CACGTG 1 cut(s) 60
PpsI GAGTC 3 cut(s) 142, 254, 529
Ppu21I YACGTR 1 cut(s) 60
PscI ACATGT 1 cut(s) 309
PshBI ATTAAT 1 cut(s) 627
PsiI TTATAA 1 cut(s) 872
Psp124BI GAGCTC 1 cut(s) 21
PspCI CACGTG 1 cut(s) 60
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 3 cut(s) 223, 235, 470
PsrI GAACNNNNNNTAC 2 cut(s) 659, 691
PsuI RGATCY 2 cut(s) 332, 554
RsaI GTAC 7 cut(s) 182, 462, 588, 597, 618, 635, 668
RsaNI GTAC 7 cut(s) 181, 461, 587, 596, 617, 634, 667
RseI CAYNNNNRTG 4 cut(s) 72, 314, 354, 804
SacI GAGCTC 1 cut(s) 21
SaqAI TTAA 3 cut(s) 542, 627, 877
SatI GCNGC 3 cut(s) 55, 106, 550
Sau3AI GATC 5 cut(s) 64, 267, 332, 554, 682
Sau96I GGNCC 3 cut(s) 223, 235, 470
ScaI AGTACT 1 cut(s) 618
SchI GAGTC 3 cut(s) 143, 255, 530
ScrFI CCNGG 1 cut(s) 559
SduI GDGCHC 2 cut(s) 21, 351
SfaNI GCATC 2 cut(s) 646, 851
SinI GGWCC 2 cut(s) 223, 235
SmiMI CAYNNNNRTG 4 cut(s) 72, 314, 354, 804
Sse9I AATT 9 cut(s) 5, 79, 256, 275, 544, 606, 708, 867, 874
SseBI AGGCCT 1 cut(s) 442
SsiI CCGC 4 cut(s) 55, 164, 549, 552
SspMI CTAG 1 cut(s) 464
SstI GAGCTC 1 cut(s) 21
StuI AGGCCT 1 cut(s) 442
StyD4I CCNGG 1 cut(s) 557
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 2 cut(s) 371, 566
TaiI ACGT 3 cut(s) 37, 62, 395
TaqI TCGA 2 cut(s) 270, 734
TasI AATT 9 cut(s) 5, 79, 256, 275, 544, 606, 708, 867, 874
TatI WGTACW 3 cut(s) 180, 460, 616
TauI GCSGC 2 cut(s) 57, 552
TfiI GAWTC 2 cut(s) 611, 778
Tru1I TTAA 3 cut(s) 542, 627, 877
Tru9I TTAA 3 cut(s) 542, 627, 877
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 1 cut(s) 105
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 2 cut(s) 310, 667
Van91I CCANNNNNTGG 1 cut(s) 232
VpaK11BI GGWCC 2 cut(s) 223, 235
VspI ATTAAT 1 cut(s) 627
XagI CCTNNNNNAGG 1 cut(s) 581
XceI RCATGY 2 cut(s) 313, 860
XspI CTAG 1 cut(s) 464
ZrmI AGTACT 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.