MD15G1325100.v1.1

Xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002083
Physical Location & Seq
Forward (+)
34647974 .. 34648519
546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1325100.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGACCGACCCTACTTATTTTCTTTCATCGGTGGGCCTAGAAAGGGGTTAGAAAAAGCGTCCGCTCGGGACGAGTTCTTAAGGCAATGCGGTGAGTCGACCCGCTGCATGCTTTTAAAATGTGGGTCTGGGGCTGGCAAGTGCCACGAGCCCAGCGAGGTGCTAAAGGTGATGTCCGAGTCGCAGTTCTGCTTACAAGCGCCCGGTGACTCGTTCACCCGGCGGTCGACGTTTAACTCGGTGCTCGCCTGCTGCATACCGGTTTTCTCGTCGTCGCACACGGCGTACACTCAGTACAAGTGGTTTTTACTTGGTGACGAGAGCACGTATTCGATTTACATTGATGAGAAGAGCGACGCGAGTAAGAGAATCAACGAGGAGCTGCTGAAGATTCCGAAAGAAAAGGTGACGGCGATGCGAGAAAAGCTTATAGAATTGATCCCAAGTCTGACTTACGCGCATCCGAATGCGACTAATCTTGGGTTTGGGGACGCCGTGGACGTTGCACTTGCATCGCTGGCCAAGCACGTCCAGAAAATTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

182

Amino Acids

20.12

Weight (kDa)

8.13

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 2 - 118 2.9e-21 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 65
AccI GTMKAC 2 cut(s) 98, 227
AccII CGCG 2 cut(s) 359, 458
AciI CCGC 4 cut(s) 63, 90, 103, 223
AclWI GGATC 1 cut(s) 433
AcoI YGGCCR 1 cut(s) 519
AcuI CTGAAG 1 cut(s) 407
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 2 cut(s) 287, 296
AfiI CCNNNNNNNGG 1 cut(s) 44
AflII CTTAAG 1 cut(s) 79
AgeI ACCGGT 1 cut(s) 259
AjiI CACGTC 1 cut(s) 529
AluBI AGCT 2 cut(s) 382, 427
AluI AGCT 2 cut(s) 382, 427
Alw21I GWGCWC 2 cut(s) 246, 326
AlwI GGATC 1 cut(s) 433
Ama87I CYCGRG 1 cut(s) 66
AoxI GGCC 2 cut(s) 35, 519
ApeKI GCWGC 3 cut(s) 105, 252, 382
AsiGI ACCGGT 1 cut(s) 259
AspLEI GCGC 2 cut(s) 202, 460
AspS9I GGNCC 2 cut(s) 4, 35
AsuC2I CCSGG 2 cut(s) 204, 220
AsuHPI GGTGA 6 cut(s) 104, 181, 208, 218, 326, 418
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 1 cut(s) 4
BalI TGGCCA 1 cut(s) 521
BanII GRGCYC 1 cut(s) 153
BauI CACGAG 1 cut(s) 146
Bbv12I GWGCWC 2 cut(s) 246, 326
BbvI GCAGC 3 cut(s) 92, 239, 369
BceAI ACGGC 3 cut(s) 297, 426, 479
BcgI CGANNNNNNTGC 2 cut(s) 495, 529
BcnI CCSGG 2 cut(s) 204, 220
BfaI CTAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 203
BfrI CTTAAG 1 cut(s) 79
BisI GCNGC 3 cut(s) 106, 253, 383
BlsI GCNGC 3 cut(s) 107, 254, 384
Bme1390I CCNGG 2 cut(s) 204, 220
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 66
BmgBI CACGTC 1 cut(s) 529
BmgT120I GGNCC 2 cut(s) 4, 35
BmrFI CCNGG 2 cut(s) 204, 220
BmsI GCATC 3 cut(s) 405, 469, 521
BpuMI CCSGG 2 cut(s) 204, 220
BsaAI YACGTR 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 495
BsaWI WCCGGW 1 cut(s) 259
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 259
Bse3DI GCAATG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMI GCAATG 1 cut(s) 92
BseMII CTCAG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 3 cut(s) 92, 239, 369
BseYI CCCAGC 1 cut(s) 152
Bsh1236I CGCG 2 cut(s) 359, 458
Bsh1285I CGRYCG 1 cut(s) 227
BshFI GGCC 2 cut(s) 37, 521
BshTI ACCGGT 1 cut(s) 259
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 2 cut(s) 246, 326
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 3 cut(s) 204, 220, 260
BslFI GGGAC 2 cut(s) 83, 503
BslI CCNNNNNNNGG 1 cut(s) 44
BsmFI GGGAC 2 cut(s) 83, 503
BsmI GAATGC 1 cut(s) 472
BsnI GGCC 2 cut(s) 37, 521
BsoBI CYCGRG 1 cut(s) 66
Bsp1286I GDGCHC 3 cut(s) 153, 246, 326
Bsp143I GATC 1 cut(s) 438
BspACI CCGC 4 cut(s) 63, 90, 103, 223
BspANI GGCC 2 cut(s) 37, 521
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 2 cut(s) 359, 458
BspPI GGATC 1 cut(s) 433
BspQI GCTCTTC 1 cut(s) 344
BspTI CTTAAG 1 cut(s) 79
BsrBI CCGCTC 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 92
BsrFI RCCGGY 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 259
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 1 cut(s) 438
BssNI GRCGYC 1 cut(s) 492
BssSI CACGAG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 344
BstACI GRCGYC 1 cut(s) 492
BstAFI CTTAAG 1 cut(s) 79
BstBAI YACGTR 1 cut(s) 327
BstC8I GCNNGC 5 cut(s) 110, 136, 246, 250, 519
BstDEI CTNAG 1 cut(s) 291
BstDSI CCRYGG 1 cut(s) 495
BstF5I GGATG 1 cut(s) 460
BstFNI CGCG 2 cut(s) 359, 458
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 2 cut(s) 202, 460
BstKTI GATC 1 cut(s) 441
BstMBI GATC 1 cut(s) 438
BstMCI CGRYCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 3 cut(s) 424, 518, 523
BstNSI RCATGY 1 cut(s) 112
BstSCI CCNGG 2 cut(s) 202, 218
BstUI CGCG 2 cut(s) 359, 458
BstV1I GCAGC 3 cut(s) 92, 239, 369
BsuRI GGCC 2 cut(s) 37, 521
BtgI CCRYGG 1 cut(s) 495
BtgZI GCGATG 2 cut(s) 428, 498
BtrI CACGTC 1 cut(s) 529
BtsCI GGATG 1 cut(s) 460
Cac8I GCNNGC 5 cut(s) 110, 136, 246, 250, 519
CfoI GCGC 2 cut(s) 202, 460
Cfr10I RCCGGY 1 cut(s) 259
Cfr13I GGNCC 2 cut(s) 4, 35
CseI GACGC 3 cut(s) 48, 365, 500
Csp6I GTAC 2 cut(s) 286, 295
CspAI ACCGGT 1 cut(s) 259
CviAII CATG 1 cut(s) 109
CviJI RGCY 6 cut(s) 37, 134, 151, 382, 427, 521
CviKI_1 RGCY 6 cut(s) 37, 134, 151, 382, 427, 521
CviQI GTAC 2 cut(s) 286, 295
DdeI CTNAG 1 cut(s) 291
DpnI GATC 1 cut(s) 440
DpnII GATC 1 cut(s) 438
DraI TTTAAA 1 cut(s) 117
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
Eco24I GRGCYC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 153
FaeI CATG 1 cut(s) 112
FaiI YATR 5 cut(s) 110, 257, 431, 542, 544
FalI AAGNNNNNCTT 2 cut(s) 436, 468
FaqI GGGAC 2 cut(s) 83, 503
FatI CATG 1 cut(s) 108
FauI CCCGC 1 cut(s) 110
FblI GTMKAC 2 cut(s) 98, 227
Fnu4HI GCNGC 3 cut(s) 106, 253, 383
FokI GGATG 1 cut(s) 447
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 3 cut(s) 106, 253, 383
FspBI CTAG 1 cut(s) 39
GlaI GCGC 2 cut(s) 201, 459
GluI GCNGC 3 cut(s) 106, 253, 383
GsaI CCCAGC 1 cut(s) 156
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 2 cut(s) 37, 521
HapII CCGG 3 cut(s) 204, 220, 260
HgaI GACGC 3 cut(s) 48, 365, 500
HhaI GCGC 2 cut(s) 202, 460
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 1 cut(s) 112
Hin6I GCGC 2 cut(s) 200, 458
HinP1I GCGC 2 cut(s) 200, 458
HincII GTYRAC 2 cut(s) 99, 228
HindII GTYRAC 2 cut(s) 99, 228
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 5 cut(s) 95, 179, 209, 369, 391
HpaII CCGG 3 cut(s) 204, 220, 260
HphI GGTGA 6 cut(s) 104, 181, 208, 218, 326, 418
Hpy166II GTNNAC 5 cut(s) 99, 216, 228, 288, 499
Hpy188I TCNGA 4 cut(s) 178, 396, 450, 465
Hpy188III TCNNGA 2 cut(s) 68, 532
Hpy8I GTNNAC 5 cut(s) 99, 216, 228, 288, 499
Hpy99I CGWCG 4 cut(s) 232, 274, 277, 359
HpyCH4IV ACGT 4 cut(s) 230, 326, 501, 528
HpyCH4V TGCA 4 cut(s) 108, 255, 506, 512
HpyF10VI GCNNNNNNNGC 3 cut(s) 424, 518, 523
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 4 cut(s) 230, 326, 501, 528
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 1 cut(s) 112
HspAI GCGC 2 cut(s) 200, 458
Kzo9I GATC 1 cut(s) 438
LguI GCTCTTC 1 cut(s) 344
LmnI GCTCC 1 cut(s) 379
LpnPI CCDG 8 cut(s) 114, 120, 166, 217, 233, 262, 273, 503
Lsp1109I GCAGC 3 cut(s) 92, 239, 369
LweI GCATC 3 cut(s) 405, 469, 521
MaeI CTAG 1 cut(s) 39
MaeII ACGT 4 cut(s) 230, 326, 501, 528
MaeIII GTNAC 3 cut(s) 206, 314, 406
MalI GATC 1 cut(s) 440
MbiI CCGCTC 1 cut(s) 65
MboI GATC 1 cut(s) 438
MboII GAAGA 2 cut(s) 361, 400
MhlI GDGCHC 3 cut(s) 153, 246, 326
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 2 cut(s) 434, 537
MluNI TGGCCA 1 cut(s) 521
MlyI GAGTC 3 cut(s) 104, 188, 203
MnlI CCTC 2 cut(s) 151, 370
Mox20I TGGCCA 1 cut(s) 521
MscI TGGCCA 1 cut(s) 521
MseI TTAA 3 cut(s) 80, 116, 234
MslI CAYNNNNRTG 1 cut(s) 465
Msp20I TGGCCA 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 105
MspCI CTTAAG 1 cut(s) 79
MspI CCGG 3 cut(s) 204, 220, 260
MspR9I CCNGG 2 cut(s) 204, 220
Mva1269I GAATGC 1 cut(s) 472
MvnI CGCG 2 cut(s) 359, 458
MwoI GCNNNNNNNGC 3 cut(s) 424, 518, 523
NciI CCSGG 2 cut(s) 204, 220
NdeII GATC 1 cut(s) 438
NlaIII CATG 1 cut(s) 112
NmuCI GTSAC 3 cut(s) 206, 314, 406
NspI RCATGY 1 cut(s) 112
PaeI GCATGC 1 cut(s) 112
PciSI GCTCTTC 1 cut(s) 344
PcsI WCGNNNNNNNCGW 3 cut(s) 153, 281, 498
PctI GAATGC 1 cut(s) 472
PfeI GAWTC 2 cut(s) 369, 391
PinAI ACCGGT 1 cut(s) 259
PkrI GCNGC 3 cut(s) 107, 254, 384
PleI GAGTC 3 cut(s) 103, 187, 203
PpsI GAGTC 3 cut(s) 103, 187, 203
Ppu21I YACGTR 1 cut(s) 327
PspFI CCCAGC 1 cut(s) 152
PspPI GGNCC 2 cut(s) 4, 35
RsaI GTAC 2 cut(s) 287, 296
RsaNI GTAC 2 cut(s) 286, 295
RseI CAYNNNNRTG 1 cut(s) 465
SalI GTCGAC 2 cut(s) 97, 226
SapI GCTCTTC 1 cut(s) 344
SaqAI TTAA 3 cut(s) 80, 116, 234
SatI GCNGC 3 cut(s) 106, 253, 383
Sau3AI GATC 1 cut(s) 438
Sau96I GGNCC 2 cut(s) 4, 35
SchI GAGTC 3 cut(s) 104, 188, 203
ScrFI CCNGG 2 cut(s) 204, 220
SduI GDGCHC 3 cut(s) 153, 246, 326
SetI ASST 9 cut(s) 162, 171, 233, 329, 384, 408, 429, 504, 531
SfaNI GCATC 3 cut(s) 405, 469, 521
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 465
SmlI CTYRAG 1 cut(s) 79
SmoI CTYRAG 1 cut(s) 79
SphI GCATGC 1 cut(s) 112
Sse9I AATT 2 cut(s) 434, 537
SsiI CCGC 4 cut(s) 63, 90, 103, 223
SspMI CTAG 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 202, 218
TaiI ACGT 4 cut(s) 233, 329, 504, 531
TaqI TCGA 3 cut(s) 98, 227, 332
TaqII GACCGA 1 cut(s) 21
TasI AATT 2 cut(s) 434, 537
TatI WGTACW 1 cut(s) 294
TfiI GAWTC 2 cut(s) 369, 391
Tru1I TTAA 3 cut(s) 80, 116, 234
Tru9I TTAA 3 cut(s) 80, 116, 234
TseFI GTSAC 3 cut(s) 206, 314, 406
TseI GCWGC 3 cut(s) 105, 252, 382
Tsp45I GTSAC 3 cut(s) 206, 314, 406
TspDTI ATGAA 1 cut(s) 16
Vha464I CTTAAG 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 4
XceI RCATGY 1 cut(s) 112
XmiI GTMKAC 2 cut(s) 98, 227
XspI CTAG 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.