MD15G1326800.v1.1

DNA-binding domain in plant proteins such as APETALA2 and EREBPs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013978
Physical Location & Seq
Reverse (-)
35297146 .. 35298843
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1326800.v1.1.491

Sequence Viewer

Length: 972 bp
ATGGCAACATCCATAGATCTTTACAGCAGCAGCACAATGACATCAGATCTATCAGATCCCTCGAGGGAAGATCAGCTCATGAAAGCACTTATGCCTTATATGAAAAGTGCTTCTTCAACATCCCCTTCCACTTCTTACACTTACCCTCCTCAGAACAACCAAAACTTCAACTTGTACCCTGACTTCTGCTCAGCACCTATGATTTCACAAGCAGGTGCTATAGGGCTTAACCAACTTACCCCATCTCAAATTCTTCAAATCCAATCCCAAATGATCCTCCAACAACACCAGCAAAAACAACAGATTTCTGCTTTTGCTTCCTCACCTGCTCACACCCTTAAGCTGCTTCGTCCAAAAGCAGTTCCAATGAAGCAAGCTGGCACTCCCTCCAAACCCACAAAGCTCTACAGAGGAGTGAGGCAGAGGCATTGGGGCAAATGGGTTGCTGAGATTAGACTTCCCAGGAACCGAACCCGGCTTTGGCTTGGTACATTTGACACCGCTGAAGAAGCTGCTTTGGCTTATGATAAAGCTGCTTTTAAGCTTAGGGGAGACTTTGCTCGCCTCAATTTCCCCCATCTCAGGCACCAGGGAGCCCTTGTCTGCGGCGACTTTGGCCACTACAAGCCCCTGCATTCCTCTGTCGATGCGAAGCTCCAGGCAATATGCCAGAGCTTGGGTGCTAATTCGACGAAACAGGGGAATACAGGGGAGCCCTGTCCTGTGGCAGAGACAAAGCCAGTGGTATCAGCTCCTTTAGAGGCTAAGATGGATGATTCATTCAAAAGTGAATTGAAAAGCGAATCGGAGGCCTTTTCTTCGTCGTCATATTCGCCTTCTCGGTCTGATGAGTCATCTTCGCCGGAATCCGACATTACCTTCTTAGATTTCTCGGATTCTCAGTGGGACGAGGCTGAGAATTTTGGATTGGAGAAGTACCCTTCAGTGGAGATTGATTGGTCTGCTATCTAA

Protein Analysis

324

Amino Acids

35.76

Weight (kDa)

6.39

Isoelectric Point (pI)

56.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 135 - 184 1.4e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22200 AT4G39780
fragaria_vesca FvH4_1g21210
malus_domestica MD15G1326800.v1.1
prunus_persica Prupe.6G182200_v2.0.a1
pyrus_communis pycom01g05500 pycom395g00070
rosa_chinensis RchiOBHm_Chr2g0115041
rosa_laevigata RLG00000018150
rosa_multiflora Rmu_sc0000784.1_g000017
rosa_roxburghii Rroxscaffold_2G00130510
rosa_rugosa Rorug02G0192600
rosa_samantha Rh2AG251200 Rh2BG260700 Rh2CG255800 Rh2DG259200
rosa_wichuraiana Rw2G019610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 203, 334
AbsI CCTCGAGG 1 cut(s) 61
Acc36I ACCTGC 2 cut(s) 203, 334
AccB1I GGYRCC 1 cut(s) 585
AccB7I CCANNNNNTGG 1 cut(s) 676
AciI CCGC 2 cut(s) 501, 606
AclWI GGATC 2 cut(s) 50, 268
AcoI YGGCCR 1 cut(s) 616
AcsI RAATTY 2 cut(s) 249, 919
AcuI CTGAAG 2 cut(s) 525, 927
AfaI GTAC 3 cut(s) 176, 490, 938
AfiI CCNNNNNNNGG 4 cut(s) 480, 582, 676, 946
AflII CTTAAG 1 cut(s) 338
AgsI TTSAA 5 cut(s) 117, 169, 257, 784, 796
AjnI CCWGG 3 cut(s) 461, 588, 657
AjuI GAANNNNNNNTTGG 4 cut(s) 463, 495, 911, 943
Alw26I GTCTC 2 cut(s) 546, 725
AlwI GGATC 2 cut(s) 50, 268
Ama87I CYCGRG 1 cut(s) 61
AoxI GGCC 2 cut(s) 616, 810
ApeKI GCWGC 5 cut(s) 27, 30, 343, 512, 533
ApoI RAATTY 2 cut(s) 249, 919
AsuC2I CCSGG 1 cut(s) 475
AsuHPI GGTGA 1 cut(s) 315
AvaI CYCGRG 1 cut(s) 61
BalI TGGCCA 1 cut(s) 618
BanI GGYRCC 1 cut(s) 585
BanII GRGCYC 2 cut(s) 598, 717
BarI GAAGNNNNNNTAC 2 cut(s) 167, 199
BbvI GCAGC 5 cut(s) 39, 42, 330, 499, 520
BccI CCATC 3 cut(s) 250, 585, 763
BciT130I CCWGG 3 cut(s) 463, 590, 659
BcnI CCSGG 1 cut(s) 475
BcoDI GTCTC 2 cut(s) 546, 725
BfmI CTRYAG 2 cut(s) 219, 406
BfrI CTTAAG 1 cut(s) 338
BfuAI ACCTGC 2 cut(s) 203, 334
BglII AGATCT 2 cut(s) 16, 46
BisI GCNGC 6 cut(s) 28, 31, 344, 513, 534, 607
BlpI GCTNAGC 1 cut(s) 190
BlsI GCNGC 6 cut(s) 29, 32, 345, 514, 535, 608
Bme1390I CCNGG 4 cut(s) 463, 475, 590, 659
BmeT110I CYCGRG 1 cut(s) 61
BmiI GGNNCC 4 cut(s) 467, 587, 595, 714
BmrFI CCNGG 4 cut(s) 463, 475, 590, 659
BmsI GCATC 1 cut(s) 637
BpmI CTGGAG 1 cut(s) 641
Bpu10I CCTNAGC 1 cut(s) 545
Bpu1102I GCTNAGC 1 cut(s) 190
BpuMI CCSGG 1 cut(s) 475
BsaJI CCNNGG 2 cut(s) 461, 589
BsaXI ACNNNNNCTCC 4 cut(s) 130, 160, 585, 615
Bsc4I CCNNNNNNNGG 4 cut(s) 480, 582, 676, 946
Bse1I ACTGG 1 cut(s) 740
BseBI CCWGG 3 cut(s) 463, 590, 659
BseDI CCNNGG 2 cut(s) 461, 589
BseGI GGATG 3 cut(s) 8, 119, 778
BseLI CCNNNNNNNGG 4 cut(s) 480, 582, 676, 946
BseMII CTCAG 6 cut(s) 164, 204, 438, 595, 906, 914
BseNI ACTGG 1 cut(s) 740
BseRI GAGGAG 2 cut(s) 138, 426
BseXI GCAGC 5 cut(s) 39, 42, 330, 499, 520
BshFI GGCC 2 cut(s) 618, 812
BshNI GGYRCC 1 cut(s) 585
BsiHKCI CYCGRG 1 cut(s) 61
BsiSI CCGG 2 cut(s) 475, 863
BslFI GGGAC 1 cut(s) 920
BslI CCNNNNNNNGG 4 cut(s) 480, 582, 676, 946
BsmAI GTCTC 2 cut(s) 546, 725
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 2 cut(s) 618, 812
BsoBI CYCGRG 1 cut(s) 61
Bsp1286I GDGCHC 2 cut(s) 598, 717
Bsp143I GATC 5 cut(s) 16, 46, 55, 70, 273
Bsp1720I GCTNAGC 1 cut(s) 190
BspACI CCGC 2 cut(s) 501, 606
BspANI GGCC 2 cut(s) 618, 812
BspCNI CTCAG 6 cut(s) 163, 203, 439, 594, 907, 913
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 4 cut(s) 467, 587, 595, 714
BspMI ACCTGC 2 cut(s) 203, 334
BspPI GGATC 2 cut(s) 50, 268
BspT107I GGYRCC 1 cut(s) 585
BspTI CTTAAG 1 cut(s) 338
BsrI ACTGG 1 cut(s) 740
BssECI CCNNGG 2 cut(s) 461, 589
BssMI GATC 5 cut(s) 16, 46, 55, 70, 273
Bst2UI CCWGG 3 cut(s) 463, 590, 659
BstAFI CTTAAG 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 375, 379, 562
BstDEI CTNAG 9 cut(s) 150, 190, 447, 545, 581, 765, 883, 900, 915
BstF5I GGATG 3 cut(s) 8, 119, 778
BstKTI GATC 5 cut(s) 19, 49, 58, 73, 276
BstMAI GTCTC 2 cut(s) 546, 725
BstMBI GATC 5 cut(s) 16, 46, 55, 70, 273
BstMWI GCNNNNNNNGC 3 cut(s) 509, 518, 615
BstNI CCWGG 3 cut(s) 463, 590, 659
BstSCI CCNGG 4 cut(s) 461, 473, 588, 657
BstSFI CTRYAG 2 cut(s) 219, 406
BstV1I GCAGC 5 cut(s) 39, 42, 330, 499, 520
BstX2I RGATCY 3 cut(s) 16, 46, 55
BstYI RGATCY 3 cut(s) 16, 46, 55
BsuRI GGCC 2 cut(s) 618, 812
BtsCI GGATG 3 cut(s) 8, 119, 778
BtsIMutI CAGTG 3 cut(s) 747, 908, 951
BveI ACCTGC 2 cut(s) 203, 334
Cac8I GCNNGC 3 cut(s) 375, 379, 562
CciI TCATGA 1 cut(s) 78
Csp6I GTAC 3 cut(s) 175, 489, 937
CspCI CAANNNNNGTGG 2 cut(s) 723, 758
CviAII CATG 1 cut(s) 79
CviQI GTAC 3 cut(s) 175, 489, 937
DdeI CTNAG 9 cut(s) 150, 190, 447, 545, 581, 765, 883, 900, 915
DpnI GATC 5 cut(s) 18, 48, 57, 72, 275
DpnII GATC 5 cut(s) 16, 46, 55, 70, 273
EaeI YGGCCR 1 cut(s) 616
Eco147I AGGCCT 1 cut(s) 812
Eco24I GRGCYC 2 cut(s) 598, 717
Eco57I CTGAAG 2 cut(s) 525, 927
Eco88I CYCGRG 1 cut(s) 61
EcoRII CCWGG 3 cut(s) 461, 588, 657
EcoT38I GRGCYC 2 cut(s) 598, 717
FaeI CATG 1 cut(s) 82
FalI AAGNNNNNCTT 2 cut(s) 97, 129
FaqI GGGAC 1 cut(s) 920
FatI CATG 1 cut(s) 78
Fnu4HI GCNGC 6 cut(s) 28, 31, 344, 513, 534, 607
FokI GGATG 2 cut(s) 106, 785
FriOI GRGCYC 2 cut(s) 598, 717
Fsp4HI GCNGC 6 cut(s) 28, 31, 344, 513, 534, 607
GluI GCNGC 6 cut(s) 28, 31, 344, 513, 534, 607
GsuI CTGGAG 1 cut(s) 641
HaeIII GGCC 2 cut(s) 618, 812
HapII CCGG 2 cut(s) 475, 863
Hin1II CATG 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 5 cut(s) 776, 803, 851, 866, 896
HpaII CCGG 2 cut(s) 475, 863
HphI GGTGA 1 cut(s) 315
Hpy188I TCNGA 7 cut(s) 46, 55, 153, 808, 847, 871, 895
Hpy188III TCNNGA 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 694, 826
HpyAV CCTTC 4 cut(s) 135, 846, 889, 951
HpyCH4V TGCA 1 cut(s) 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 509, 518, 615
HpyF3I CTNAG 9 cut(s) 150, 190, 447, 545, 581, 765, 883, 900, 915
Hsp92II CATG 1 cut(s) 82
Kzo9I GATC 5 cut(s) 16, 46, 55, 70, 273
LmnI GCTCC 4 cut(s) 593, 660, 712, 757
Lsp1109I GCAGC 5 cut(s) 39, 42, 330, 499, 520
LweI GCATC 1 cut(s) 637
MalI GATC 5 cut(s) 18, 48, 57, 72, 275
MboI GATC 5 cut(s) 16, 46, 55, 70, 273
MboII GAAGA 6 cut(s) 80, 105, 245, 518, 810, 849
MflI RGATCY 3 cut(s) 16, 46, 55
MhlI GDGCHC 2 cut(s) 598, 717
MlsI TGGCCA 1 cut(s) 618
MluCI AATT 5 cut(s) 249, 568, 685, 791, 919
MluNI TGGCCA 1 cut(s) 618
MlyI GAGTC 1 cut(s) 860
MmeI TCCRAC 2 cut(s) 304, 894
Mox20I TGGCCA 1 cut(s) 618
MscI TGGCCA 1 cut(s) 618
MseI TTAA 3 cut(s) 228, 339, 540
Msp20I TGGCCA 1 cut(s) 618
MspA1I CMGCKG 1 cut(s) 503
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 2 cut(s) 475, 863
MspR9I CCNGG 4 cut(s) 463, 475, 590, 659
Mva1269I GAATGC 1 cut(s) 634
MvaI CCWGG 3 cut(s) 463, 590, 659
MwoI GCNNNNNNNGC 3 cut(s) 509, 518, 615
NciI CCSGG 1 cut(s) 475
NdeII GATC 5 cut(s) 16, 46, 55, 70, 273
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 4 cut(s) 467, 587, 595, 714
PaeR7I CTCGAG 1 cut(s) 61
PagI TCATGA 1 cut(s) 78
PaqCI CACCTGC 2 cut(s) 203, 334
PceI AGGCCT 1 cut(s) 812
PctI GAATGC 1 cut(s) 634
PfeI GAWTC 4 cut(s) 776, 803, 866, 896
PflMI CCANNNNNTGG 1 cut(s) 676
PkrI GCNGC 6 cut(s) 29, 32, 345, 514, 535, 608
PleI GAGTC 1 cut(s) 859
PpsI GAGTC 1 cut(s) 859
Psp6I CCWGG 3 cut(s) 461, 588, 657
PspGI CCWGG 3 cut(s) 461, 588, 657
PspN4I GGNNCC 4 cut(s) 467, 587, 595, 714
PspXI VCTCGAGB 1 cut(s) 61
PsuI RGATCY 3 cut(s) 16, 46, 55
RsaI GTAC 3 cut(s) 176, 490, 938
RsaNI GTAC 3 cut(s) 175, 489, 937
SaqAI TTAA 3 cut(s) 228, 339, 540
SatI GCNGC 6 cut(s) 28, 31, 344, 513, 534, 607
Sau3AI GATC 5 cut(s) 16, 46, 55, 70, 273
SchI GAGTC 1 cut(s) 860
ScrFI CCNGG 4 cut(s) 463, 475, 590, 659
SduI GDGCHC 2 cut(s) 598, 717
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 2 cut(s) 219, 406
Sfr274I CTCGAG 1 cut(s) 61
SlaI CTCGAG 1 cut(s) 61
SmlI CTYRAG 2 cut(s) 61, 338
SmoI CTYRAG 2 cut(s) 61, 338
Sse9I AATT 5 cut(s) 249, 568, 685, 791, 919
SseBI AGGCCT 1 cut(s) 812
SsiI CCGC 2 cut(s) 501, 606
StuI AGGCCT 1 cut(s) 812
StyD4I CCNGG 4 cut(s) 461, 473, 588, 657
TaqI TCGA 3 cut(s) 62, 645, 689
TaqII GACCGA 1 cut(s) 831
TasI AATT 5 cut(s) 249, 568, 685, 791, 919
TauI GCSGC 1 cut(s) 609
TfiI GAWTC 4 cut(s) 776, 803, 866, 896
Tru1I TTAA 3 cut(s) 228, 339, 540
Tru9I TTAA 3 cut(s) 228, 339, 540
TscAI CASTG 3 cut(s) 747, 908, 951
TseI GCWGC 5 cut(s) 27, 30, 343, 512, 533
TspDTI ATGAA 4 cut(s) 95, 116, 383, 768
TspRI CASTG 3 cut(s) 747, 908, 951
Van91I CCANNNNNTGG 1 cut(s) 676
Vha464I CTTAAG 1 cut(s) 338
XapI RAATTY 2 cut(s) 249, 919
XhoI CTCGAG 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.