Rorug02G0192600

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000152
Physical Location & Seq
Forward (+)
17959963 .. 17963813
3851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0192600.1

Sequence Viewer

Length: 1323 bp
ATGGCGGAGCTAAAGCTCTCGGAGAGTCGAGACTTGACCCGAATAGAGCGCATAGGCGCACACTCCCACATCCGGGGACTTGGTCTTGACTCTGCCCTAGAAGCTCGTGATGTTTCGGAGGGCATGGTGGGCCAGACGGCGGCGCGGAAGGCTGCCGGTGTGATACTACAGATGATCAAGGAAGGGAAGATAGCTGGACGGGCTGTGTTGTTGGCTGGACAGCCTGGCACCGGCAAGACGGCCATTGCGATGGGGATGGCTAAGTCACTGGGGCTTGAAACGCCGTTTGCGATGTTAGCTGGGAGTGAGCTATTTTCGCTTGAGATGGGCAAGACCGAGGCCCTGATGCAAGCTTTTAGGAAGGCTATTGGGGTGAGAATCAAGGAAGAGACTGAAATTATTGAAGGTGAGGTGGTGGAGGTCCAGATTGACCGGCCTGCGGTGGCTGGAGCAGCTTCGAAGACTGGGAAATTGACATTGAAGACGACGGAGATGGAGACGGTGTATGACTTGGGTGCCAAGATGATTGAAGCATTGGGGAAGGAGAAGGTGCAGAGTGGGGATGTGGTTGCCATTGACAAAGCGTCCGGGAAGATTACCAAGGTCGGTAGGTCATTTTCCAGGTCGAGGGACTATGATGCCATGGGACCGAGTGTGAAGTTAGTGCAGTGCCCTGACGGGGAGTTGCAGAAGCGTAAGGAGGTTGTGCATTGCGTCACACTTCATGAGATAGATGTCATCAACAGCAGAACACAAGGATTTCTGGCACTCTTTACAGGAGATACTGGTGAAATTCGTTCAGAAGTGAGAGAACAGATTGACACAAAGGTAGCAGAGTGGAGGGAGGAAGGGAAGGCAGAAATTGTGCCAGGTGTTCTCTTTATCGATGAGGTCCATATGCTTGACATTGAGTGCTTTTCATTTCTGAATCGTGCTTTGGAGAATGAGATGGCTCCAATTTTAGTTGTTGCTACGAACAGAGGGATCACTACAATCAGAGGCACAAACTATAAATCTCCCCATGGTATTCCAATTGATCTCCTTGATCGCCTGCTTATTATCACCACTCAGCCTTACACAGAGGGTGAAATTCGCAAGATTCTTGACATAAGATCCCAAGAAGAAGAAGTTGAGATGTCTGAAGAGGCAAAGCATTTGTTGACCAAGATCGGTGTAGATGCATCCTTGAGATATGCCATCCATCTCATAACAGCTGCTGCATTGGCATGCCAAAAGCGGAAGGGAAAGATTGTTGAGATGGAGGATATTAACCGAGTTTACCATCTGTTTTTGGATGTGACAGAGTCAGTACATGTTCAATGA

Protein Analysis

440

Amino Acids

48.2

Weight (kDa)

5.73

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIP49 PF06068 17 - 361 1.4e-165 TIP49 P-loop domain
DnaB_C PF03796 61 - 193 1.2e-06 DnaB-like helicase C terminal domain
AAA PF00004 69 - 120 3.3e-06 ATPase family associated with various cellular activities (AAA)
TIP49_C PF17856 367 - 432 2.3e-25 TIP49 AAA-lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000152)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17950
fragaria_vesca FvH4_1g11910 FvH4_3g04400
malus_domestica MD02G1133000.v1.1 MD15G1245600.v1.1
prunus_persica Prupe.7G167700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0314921 RchiOBHm_Chr1g0319941 RchiOBHm_Chr1g0321251 RchiOBHm_Chr1g0323021 RchiOBHm_Chr1g0324491 RchiOBHm_Chr1g0325171 RchiOBHm_Chr1g0360601 RchiOBHm_Chr1g0365451 RchiOBHm_Chr1g0381201 RchiOBHm_Chr2g0085401 RchiOBHm_Chr2g0099501 RchiOBHm_Chr2g0106981 RchiOBHm_Chr2g0130271 RchiOBHm_Chr2g0155121 RchiOBHm_Chr3g0455971 RchiOBHm_Chr3g0459501 RchiOBHm_Chr3g0478111 RchiOBHm_Chr3g0480661 RchiOBHm_Chr4g0388561 RchiOBHm_Chr4g0394421 RchiOBHm_Chr4g0424371 RchiOBHm_Chr5g0028631 RchiOBHm_Chr5g0069751 RchiOBHm_Chr5g0076691 RchiOBHm_Chr5g0076711 RchiOBHm_Chr5g0076721 RchiOBHm_Chr5g0079671 RchiOBHm_Chr5g0079731 RchiOBHm_Chr5g0079911 RchiOBHm_Chr5g0081741 RchiOBHm_Chr6g0255231 RchiOBHm_Chr6g0283251 RchiOBHm_Chr6g0300091 RchiOBHm_Chr7g0190751 RchiOBHm_Chr7g0240241
rosa_multiflora Rmu_co8441375.1_g000001 Rmu_sc0000110.1_g000027 Rmu_sc0000187.1_g000008 Rmu_sc0000240.1_g000065 Rmu_sc0000251.1_g000004 Rmu_sc0000272.1_g000025 Rmu_sc0000276.1_g000074 Rmu_sc0000284.1_g000010 Rmu_sc0000285.1_g000021 Rmu_sc0000360.1_g000034 Rmu_sc0000365.1_g000038 Rmu_sc0000368.1_g000013 Rmu_sc0000368.1_g000025 Rmu_sc0000770.1_g000044 Rmu_sc0000770.1_g000052 Rmu_sc0000795.1_g000098 Rmu_sc0000945.1_g000037 Rmu_sc0000949.1_g000023 Rmu_sc0000982.1_g000002 Rmu_sc0001424.1_g000004 Rmu_sc0001775.1_g000004 Rmu_sc0001952.1_g000010 Rmu_sc0002080.1_g000046 Rmu_sc0002141.1_g000016 Rmu_sc0002183.1_g000004 Rmu_sc0002187.1_g000040 Rmu_sc0002263.1_g000057 Rmu_sc0002351.1_g000061 Rmu_sc0002542.1_g000017 Rmu_sc0002751.1_g000003 Rmu_sc0002792.1_g000029 Rmu_sc0002798.1_g000020 Rmu_sc0002985.1_g000010 Rmu_sc0003124.1_g000019 Rmu_sc0003207.1_g000019 Rmu_sc0003237.1_g000003 Rmu_sc0003363.1_g000050 Rmu_sc0003443.1_g000025 Rmu_sc0003465.1_g000032 Rmu_sc0003500.1_g000012 Rmu_sc0003809.1_g000026 Rmu_sc0003859.1_g000032 Rmu_sc0004085.1_g000003 Rmu_sc0004085.1_g000008 Rmu_sc0004571.1_g000019 Rmu_sc0004753.1_g000006 Rmu_sc0004865.1_g000017 Rmu_sc0004881.1_g000007 Rmu_sc0005697.1_g000023 Rmu_sc0005812.1_g000020 Rmu_sc0007076.1_g000001 Rmu_sc0007801.1_g000003 Rmu_sc0008355.1_g000007 Rmu_sc0009380.1_g000006 Rmu_sc0010191.1_g000004 Rmu_sc0010252.1_g000006 Rmu_sc0011026.1_g000001 Rmu_sc0011824.1_g000003 Rmu_sc0012533.1_g000006 Rmu_sc0012640.1_g000006 Rmu_sc0014032.1_g000001 Rmu_sc0014545.1_g000001 Rmu_sc0014840.1_g000008 Rmu_sc0015060.1_g000013 Rmu_sc0018122.1_g000003 Rmu_sc0026563.1_g000004 Rmu_ssc0000213.1_g000057 Rmu_ssc0000213.1_g000091 Rmu_ssc0000262.1_g000021 Rmu_ssc0000282.1_g000021 Rmu_ssc0000295.1_g000041 Rmu_ssc0000438.1_g000002 Rmu_ssc0000472.1_g000015
rosa_roxburghii Rroxscaffold_1G00020080 Rroxscaffold_2G00084980 Rroxscaffold_2G00143020 Rroxscaffold_3G00261750 Rroxscaffold_3G00268610 Rroxscaffold_4G00312950 Rroxscaffold_7G00156650 Rroxscaffold_7G00215160
rosa_rugosa Rorug01G0077100 Rorug01G0201100 Rorug01G0246900 Rorug01G0256600 Rorug02G0080200 Rorug02G0089300 Rorug02G0090700 Rorug02G0177200 Rorug02G0187000 Rorug02G0192600 Rorug02G0232700 Rorug02G0262100 Rorug02G0328300 Rorug02G0408300 Rorug02G0641200 Rorug03G0155000 Rorug03G0185500 Rorug03G0256500 Rorug03G0291000 Rorug03G0299400 Rorug03G0317000 Rorug03G0326700 Rorug03G0330800 Rorug03G0339700 Rorug03G0352900 Rorug04G0029800 Rorug04G0029900 Rorug04G0048100 Rorug04G0075800 Rorug04G0124400 Rorug04G0212100 Rorug04G0212400 Rorug04G0253200 Rorug05G0116700 Rorug05G0146500 Rorug05G0211900 Rorug05G0215700 Rorug05G0216100 Rorug05G0220500 Rorug05G0339400 Rorug05G0352000 Rorug05G0356500.1 Rorug05G0398900 Rorug05G0474700 Rorug05G0511400 Rorug05G0540200 Rorug05G0541000 Rorug05G0572900.1 Rorug05G0584000 Rorug06G0000900 Rorug06G0014600 Rorug06G0018800 Rorug06G0026100 Rorug06G0042900 Rorug06G0082600 Rorug06G0133900 Rorug06G0229100 Rorug07G0321400 Rorug07G0326400 Rorug07G0326600.1
rosa_wichuraiana Rw0G017710 Rw1G012170 Rw1G039350 Rw2G010030 Rw3G024430 Rw5G045720 Rw7G025340 Rw7G040870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 227, 515
AccII CGCG 1 cut(s) 145
AciI CCGC 5 cut(s) 5, 140, 145, 440, 1237
AclWI GGATC 2 cut(s) 992, 1107
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 2 cut(s) 792, 1089
AcuI CTGAAG 1 cut(s) 1161
AfaI GTAC 1 cut(s) 1311
AfiI CCNNNNNNNGG 7 cut(s) 72, 73, 139, 230, 439, 627, 679
AflIII ACRYGT 1 cut(s) 1312
AgsI TTSAA 5 cut(s) 278, 404, 481, 530, 1319
AhdI GACNNNNNGTC 1 cut(s) 583
AjnI CCWGG 3 cut(s) 223, 620, 868
AjuI GAANNNNNNNTTGG 2 cut(s) 920, 952
AluBI AGCT 9 cut(s) 10, 16, 104, 194, 299, 310, 353, 455, 1214
AluI AGCT 9 cut(s) 10, 16, 104, 194, 299, 310, 353, 455, 1214
Alw26I GTCTC 3 cut(s) 24, 383, 491
AlwI GGATC 2 cut(s) 992, 1107
AlwNI CAGNNNCTG 1 cut(s) 1217
AoxI GGCC 4 cut(s) 130, 240, 339, 434
ApeKI GCWGC 4 cut(s) 152, 452, 1214, 1217
ApoI RAATTY 2 cut(s) 792, 1089
AspLEI GCGC 3 cut(s) 51, 59, 145
AspS9I GGNCC 5 cut(s) 130, 340, 421, 647, 892
AsuC2I CCSGG 2 cut(s) 74, 589
AsuHPI GGTGA 5 cut(s) 385, 419, 800, 1054, 1097
AsuII TTCGAA 1 cut(s) 458
AvaII GGWCC 3 cut(s) 421, 647, 892
BaeGI GKGCMC 1 cut(s) 674
BanI GGYRCC 2 cut(s) 227, 515
BauI CACGAG 1 cut(s) 105
BbsI GAAGAC 2 cut(s) 467, 488
BbvI GCAGC 4 cut(s) 139, 464, 1201, 1204
BccI CCATC 9 cut(s) 244, 250, 319, 487, 943, 1205, 1209, 1252, 1290
BceAI ACGGC 3 cut(s) 153, 255, 268
BciT130I CCWGG 3 cut(s) 225, 622, 870
BclI TGATCA 1 cut(s) 174
BcnI CCSGG 2 cut(s) 74, 589
BcoDI GTCTC 3 cut(s) 24, 383, 491
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 167
BisI GCNGC 5 cut(s) 141, 153, 453, 1215, 1218
BlsI GCNGC 5 cut(s) 142, 154, 454, 1216, 1219
Bme1390I CCNGG 5 cut(s) 74, 225, 589, 622, 870
Bme18I GGWCC 3 cut(s) 421, 647, 892
BmeRI GACNNNNNGTC 1 cut(s) 583
BmgT120I GGNCC 5 cut(s) 130, 340, 421, 647, 892
BmiI GGNNCC 4 cut(s) 229, 517, 648, 954
BmrFI CCNGG 5 cut(s) 74, 225, 589, 622, 870
BmrI ACTGGG 2 cut(s) 278, 474
BmsI GCATC 4 cut(s) 336, 628, 1168, 1190
BmuI ACTGGG 2 cut(s) 278, 474
BpiI GAAGAC 2 cut(s) 467, 488
BpmI CTGGAG 1 cut(s) 468
Bpu14I TTCGAA 1 cut(s) 458
BpuEI CTTGAG 2 cut(s) 341, 1207
BpuMI CCSGG 2 cut(s) 74, 589
Bsa29I ATCGAT 1 cut(s) 885
BsaJI CCNNGG 5 cut(s) 73, 336, 600, 642, 1021
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 7 cut(s) 72, 73, 139, 230, 439, 627, 679
Bse118I RCCGGY 3 cut(s) 155, 230, 432
Bse1I ACTGG 3 cut(s) 273, 469, 790
Bse3DI GCAATG 2 cut(s) 243, 709
BseBI CCWGG 3 cut(s) 225, 622, 870
BseCI ATCGAT 1 cut(s) 885
BseDI CCNNGG 5 cut(s) 73, 336, 600, 642, 1021
BseGI GGATG 6 cut(s) 69, 261, 568, 1181, 1197, 1300
BseLI CCNNNNNNNGG 7 cut(s) 72, 73, 139, 230, 439, 627, 679
BseMI GCAATG 2 cut(s) 243, 709
BseMII CTCAG 1 cut(s) 1082
BseNI ACTGG 3 cut(s) 273, 469, 790
BseSI GKGCMC 1 cut(s) 674
BseXI GCAGC 4 cut(s) 139, 464, 1201, 1204
BseYI CCCAGC 1 cut(s) 299
BsgI GTGCAG 2 cut(s) 572, 686
Bsh1236I CGCG 1 cut(s) 145
BshFI GGCC 4 cut(s) 132, 242, 341, 436
BshNI GGYRCC 2 cut(s) 227, 515
BshVI ATCGAT 1 cut(s) 885
BsiSI CCGG 5 cut(s) 73, 156, 231, 433, 588
BslFI GGGAC 3 cut(s) 90, 644, 660
BslI CCNNNNNNNGG 7 cut(s) 72, 73, 139, 230, 439, 627, 679
BsmAI GTCTC 3 cut(s) 24, 383, 491
BsmBI CGTCTC 1 cut(s) 491
BsmFI GGGAC 3 cut(s) 90, 644, 660
BsnI GGCC 4 cut(s) 132, 242, 341, 436
Bsp119I TTCGAA 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 674
Bsp143I GATC 6 cut(s) 174, 984, 1036, 1045, 1112, 1167
Bsp19I CCATGG 2 cut(s) 642, 1021
BspACI CCGC 5 cut(s) 5, 140, 145, 440, 1237
BspANI GGCC 4 cut(s) 132, 242, 341, 436
BspCNI CTCAG 1 cut(s) 1081
BspDI ATCGAT 1 cut(s) 885
BspFNI CGCG 1 cut(s) 145
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 4 cut(s) 229, 517, 648, 954
BspPI GGATC 2 cut(s) 992, 1107
BspT104I TTCGAA 1 cut(s) 458
BspT107I GGYRCC 2 cut(s) 227, 515
BsrDI GCAATG 2 cut(s) 243, 709
BsrFI RCCGGY 3 cut(s) 155, 230, 432
BsrI ACTGG 3 cut(s) 273, 469, 790
BssAI RCCGGY 3 cut(s) 155, 230, 432
BssECI CCNNGG 5 cut(s) 73, 336, 600, 642, 1021
BssMI GATC 6 cut(s) 174, 984, 1036, 1045, 1112, 1167
BssSI CACGAG 1 cut(s) 105
BssT1I CCWWGG 3 cut(s) 600, 642, 1021
Bst2BI CACGAG 1 cut(s) 105
Bst2UI CCWGG 3 cut(s) 225, 622, 870
Bst4CI ACNGT 1 cut(s) 502
Bst6I CTCTTC 2 cut(s) 381, 1137
BstBI TTCGAA 1 cut(s) 458
BstC8I GCNNGC 4 cut(s) 351, 438, 1052, 1228
BstDEI CTNAG 2 cut(s) 261, 1068
BstDSI CCRYGG 2 cut(s) 642, 1021
BstF5I GGATG 6 cut(s) 69, 261, 568, 1181, 1197, 1300
BstFNI CGCG 1 cut(s) 145
BstHHI GCGC 3 cut(s) 51, 59, 145
BstKTI GATC 6 cut(s) 177, 987, 1039, 1048, 1115, 1170
BstMAI GTCTC 3 cut(s) 24, 383, 491
BstMBI GATC 6 cut(s) 174, 984, 1036, 1045, 1112, 1167
BstMWI GCNNNNNNNGC 9 cut(s) 101, 129, 149, 200, 280, 296, 316, 452, 1223
BstNI CCWGG 3 cut(s) 225, 622, 870
BstNSI RCATGY 2 cut(s) 1230, 1316
BstSCI CCNGG 5 cut(s) 72, 223, 587, 620, 868
BstSFI CTRYAG 1 cut(s) 167
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 1 cut(s) 145
BstV1I GCAGC 4 cut(s) 139, 464, 1201, 1204
BstV2I GAAGAC 2 cut(s) 467, 488
BstX2I RGATCY 1 cut(s) 1112
BstXI CCANNNNNNTGG 1 cut(s) 250
BstYI RGATCY 1 cut(s) 1112
Bsu15I ATCGAT 1 cut(s) 885
BsuRI GGCC 4 cut(s) 132, 242, 341, 436
BsuTUI ATCGAT 1 cut(s) 885
BtgI CCRYGG 2 cut(s) 642, 1021
BtgZI GCGATG 2 cut(s) 263, 305
BtsCI GGATG 6 cut(s) 69, 261, 568, 1181, 1197, 1300
BtsI GCAGTG 1 cut(s) 674
BtsIMutI CAGTG 2 cut(s) 266, 674
Cac8I GCNNGC 4 cut(s) 351, 438, 1052, 1228
CaiI CAGNNNCTG 1 cut(s) 1217
CciI TCATGA 1 cut(s) 724
CfoI GCGC 3 cut(s) 51, 59, 145
Cfr10I RCCGGY 3 cut(s) 155, 230, 432
Cfr13I GGNCC 5 cut(s) 130, 340, 421, 647, 892
ClaI ATCGAT 1 cut(s) 885
CseI GACGC 2 cut(s) 573, 703
Csp6I GTAC 1 cut(s) 1310
CviAII CATG 6 cut(s) 124, 643, 725, 1022, 1227, 1313
CviQI GTAC 1 cut(s) 1310
DdeI CTNAG 2 cut(s) 261, 1068
DpnI GATC 6 cut(s) 176, 986, 1038, 1047, 1114, 1169
DpnII GATC 6 cut(s) 174, 984, 1036, 1045, 1112, 1167
DriI GACNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 2 cut(s) 381, 1137
Eam1105I GACNNNNNGTC 1 cut(s) 583
EarI CTCTTC 2 cut(s) 381, 1137
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 600, 642, 1021
Eco47I GGWCC 3 cut(s) 421, 647, 892
Eco57I CTGAAG 1 cut(s) 1161
EcoO109I RGGNCCY 1 cut(s) 340
EcoRII CCWGG 3 cut(s) 223, 620, 868
EcoT14I CCWWGG 3 cut(s) 600, 642, 1021
EcoT22I ATGCAT 1 cut(s) 1183
ErhI CCWWGG 3 cut(s) 600, 642, 1021
Esp3I CGTCTC 1 cut(s) 491
FaeI CATG 6 cut(s) 127, 646, 728, 1025, 1230, 1316
FaqI GGGAC 3 cut(s) 90, 644, 660
FatI CATG 6 cut(s) 123, 642, 724, 1021, 1226, 1312
FauNDI CATATG 1 cut(s) 897
FbaI TGATCA 1 cut(s) 174
Fnu4HI GCNGC 5 cut(s) 141, 153, 453, 1215, 1218
FokI GGATG 6 cut(s) 56, 268, 575, 1168, 1184, 1307
Fsp4HI GCNGC 5 cut(s) 141, 153, 453, 1215, 1218
FspBI CTAG 1 cut(s) 98
GlaI GCGC 3 cut(s) 50, 58, 144
GluI GCNGC 5 cut(s) 141, 153, 453, 1215, 1218
GsaI CCCAGC 1 cut(s) 303
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 4 cut(s) 132, 242, 341, 436
HapII CCGG 5 cut(s) 73, 156, 231, 433, 588
HgaI GACGC 2 cut(s) 573, 703
HhaI GCGC 3 cut(s) 51, 59, 145
Hin1II CATG 6 cut(s) 127, 646, 728, 1025, 1230, 1316
Hin6I GCGC 3 cut(s) 49, 57, 143
HinP1I GCGC 3 cut(s) 49, 57, 143
HincII GTYRAC 1 cut(s) 1161
HindII GTYRAC 1 cut(s) 1161
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 6 cut(s) 25, 89, 378, 928, 1099, 1304
HpaII CCGG 5 cut(s) 73, 156, 231, 433, 588
HphI GGTGA 5 cut(s) 385, 419, 800, 1054, 1097
Hpy166II GTNNAC 2 cut(s) 1161, 1279
Hpy188I TCNGA 6 cut(s) 22, 118, 802, 927, 998, 1141
Hpy188III TCNNGA 6 cut(s) 29, 86, 107, 424, 725, 1103
Hpy8I GTNNAC 2 cut(s) 1161, 1279
Hpy99I CGWCG 1 cut(s) 490
HpyAV CCTTC 9 cut(s) 142, 176, 355, 398, 535, 541, 842, 847, 1234
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4V TGCA 7 cut(s) 349, 553, 667, 688, 709, 1181, 1220
HpyF10VI GCNNNNNNNGC 9 cut(s) 101, 129, 149, 200, 280, 296, 316, 452, 1223
HpyF3I CTNAG 2 cut(s) 261, 1068
Hsp92II CATG 6 cut(s) 127, 646, 728, 1025, 1230, 1316
HspAI GCGC 3 cut(s) 49, 57, 143
Ksp22I TGATCA 1 cut(s) 174
Kzo9I GATC 6 cut(s) 174, 984, 1036, 1045, 1112, 1167
LmnI GCTCC 3 cut(s) 7, 449, 958
Lsp1109I GCAGC 4 cut(s) 139, 464, 1201, 1204
LweI GCATC 4 cut(s) 336, 628, 1168, 1190
MaeI CTAG 1 cut(s) 98
MaeIII GTNAC 3 cut(s) 264, 715, 1297
MalI GATC 6 cut(s) 176, 986, 1038, 1047, 1114, 1169
MboI GATC 6 cut(s) 174, 984, 1036, 1045, 1112, 1167
MboII GAAGA 8 cut(s) 199, 398, 472, 493, 604, 1133, 1136, 1154
MfeI CAATTG 1 cut(s) 1032
MflI RGATCY 1 cut(s) 1112
MhlI GDGCHC 1 cut(s) 674
MluCI AATT 7 cut(s) 396, 470, 792, 861, 957, 1032, 1089
MlyI GAGTC 3 cut(s) 34, 83, 1313
Mph1103I ATGCAT 1 cut(s) 1183
MseI TTAA 1 cut(s) 1269
MslI CAYNNNNRTG 2 cut(s) 248, 1225
MspA1I CMGCKG 1 cut(s) 1214
MspI CCGG 5 cut(s) 73, 156, 231, 433, 588
MspR9I CCNGG 5 cut(s) 74, 225, 589, 622, 870
MunI CAATTG 1 cut(s) 1032
MvaI CCWGG 3 cut(s) 225, 622, 870
MvnI CGCG 1 cut(s) 145
MwoI GCNNNNNNNGC 9 cut(s) 101, 129, 149, 200, 280, 296, 316, 452, 1223
NciI CCSGG 2 cut(s) 74, 589
NcoI CCATGG 2 cut(s) 642, 1021
NdeI CATATG 1 cut(s) 897
NdeII GATC 6 cut(s) 174, 984, 1036, 1045, 1112, 1167
NlaIII CATG 6 cut(s) 127, 646, 728, 1025, 1230, 1316
NlaIV GGNNCC 4 cut(s) 229, 517, 648, 954
NmuCI GTSAC 3 cut(s) 264, 715, 1297
NsiI ATGCAT 1 cut(s) 1183
NspI RCATGY 2 cut(s) 1230, 1316
NspV TTCGAA 1 cut(s) 458
PaeI GCATGC 1 cut(s) 1230
PagI TCATGA 1 cut(s) 724
PciI ACATGT 1 cut(s) 1312
PcsI WCGNNNNNNNCGW 2 cut(s) 245, 287
PfeI GAWTC 3 cut(s) 378, 928, 1099
PflFI GACNNNGTC 2 cut(s) 81, 1303
PfoI TCCNGGA 1 cut(s) 587
PkrI GCNGC 5 cut(s) 142, 154, 454, 1216, 1219
PleI GAGTC 3 cut(s) 33, 83, 1312
PpsI GAGTC 3 cut(s) 33, 83, 1312
PscI ACATGT 1 cut(s) 1312
Psp6I CCWGG 3 cut(s) 223, 620, 868
PspFI CCCAGC 1 cut(s) 299
PspGI CCWGG 3 cut(s) 223, 620, 868
PspN4I GGNNCC 4 cut(s) 229, 517, 648, 954
PspPI GGNCC 5 cut(s) 130, 340, 421, 647, 892
PstNI CAGNNNCTG 1 cut(s) 1217
PsuI RGATCY 1 cut(s) 1112
PsyI GACNNNGTC 2 cut(s) 81, 1303
PvuII CAGCTG 1 cut(s) 1214
RsaI GTAC 1 cut(s) 1311
RsaNI GTAC 1 cut(s) 1310
RseI CAYNNNNRTG 2 cut(s) 248, 1225
SaqAI TTAA 1 cut(s) 1269
SatI GCNGC 5 cut(s) 141, 153, 453, 1215, 1218
Sau3AI GATC 6 cut(s) 174, 984, 1036, 1045, 1112, 1167
Sau96I GGNCC 5 cut(s) 130, 340, 421, 647, 892
SchI GAGTC 3 cut(s) 34, 83, 1313
ScrFI CCNGG 5 cut(s) 74, 225, 589, 622, 870
SduI GDGCHC 1 cut(s) 674
SfaNI GCATC 4 cut(s) 336, 628, 1168, 1190
SfcI CTRYAG 1 cut(s) 167
SfuI TTCGAA 1 cut(s) 458
SinI GGWCC 3 cut(s) 421, 647, 892
SmiMI CAYNNNNRTG 2 cut(s) 248, 1225
SmlI CTYRAG 2 cut(s) 320, 1186
SmoI CTYRAG 2 cut(s) 320, 1186
SphI GCATGC 1 cut(s) 1230
Sse9I AATT 7 cut(s) 396, 470, 792, 861, 957, 1032, 1089
SsiI CCGC 5 cut(s) 5, 140, 145, 440, 1237
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 5 cut(s) 72, 223, 587, 620, 868
StyI CCWWGG 3 cut(s) 600, 642, 1021
TaaI ACNGT 1 cut(s) 502
TaqI TCGA 4 cut(s) 28, 458, 626, 885
TaqII GACCGA 2 cut(s) 350, 664
TasI AATT 7 cut(s) 396, 470, 792, 861, 957, 1032, 1089
TatI WGTACW 1 cut(s) 1309
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 3 cut(s) 378, 928, 1099
Tru1I TTAA 1 cut(s) 1269
Tru9I TTAA 1 cut(s) 1269
TscAI CASTG 2 cut(s) 273, 674
TseFI GTSAC 3 cut(s) 264, 715, 1297
TseI GCWGC 4 cut(s) 152, 452, 1214, 1217
Tsp45I GTSAC 3 cut(s) 264, 715, 1297
TspDTI ATGAA 2 cut(s) 713, 909
TspGWI ACGGA 1 cut(s) 503
TspRI CASTG 2 cut(s) 273, 674
Tth111I GACNNNGTC 2 cut(s) 81, 1303
VpaK11BI GGWCC 3 cut(s) 421, 647, 892
XapI RAATTY 2 cut(s) 792, 1089
XceI RCATGY 2 cut(s) 1230, 1316
XspI CTAG 1 cut(s) 98
Zsp2I ATGCAT 1 cut(s) 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.