Homeodomain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1925 |
| AccB1I | GGYRCC | 1 cut(s) | 1036 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 187 |
| AccII | CGCG | 1 cut(s) | 205 |
| AccIII | TCCGGA | 1 cut(s) | 1228 |
| AclWI | GGATC | 3 cut(s) | 428, 441, 442 |
| AcoI | YGGCCR | 1 cut(s) | 1528 |
| AcsI | RAATTY | 1 cut(s) | 457 |
| AcuI | CTGAAG | 2 cut(s) | 999, 1062 |
| AfaI | GTAC | 4 cut(s) | 40, 507, 591, 1913 |
| AfeI | AGCGCT | 1 cut(s) | 1114 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 97, 187, 264, 284, 387, 1143, 1661 |
| AgsI | TTSAA | 4 cut(s) | 20, 556, 1175, 1448 |
| AjnI | CCWGG | 5 cut(s) | 194, 296, 436, 1069, 1130 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 447, 479 |
| AleI | CACNNNNGTG | 1 cut(s) | 583 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1099, 1131 |
| Alw21I | GWGCWC | 2 cut(s) | 546, 640 |
| Alw26I | GTCTC | 2 cut(s) | 914, 1308 |
| AlwI | GGATC | 3 cut(s) | 428, 441, 442 |
| Ama87I | CYCGRG | 2 cut(s) | 719, 1556 |
| Aor13HI | TCCGGA | 1 cut(s) | 1228 |
| Aor51HI | AGCGCT | 1 cut(s) | 1114 |
| AoxI | GGCC | 6 cut(s) | 192, 311, 329, 378, 1519, 1528 |
| ApoI | RAATTY | 1 cut(s) | 457 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 786, 932, 1162, 1224, 1443 |
| AspLEI | GCGC | 3 cut(s) | 205, 207, 1115 |
| AspS9I | GGNCC | 8 cut(s) | 192, 312, 330, 379, 390, 564, 973, 1134 |
| AsuHPI | GGTGA | 4 cut(s) | 265, 491, 1487, 1762 |
| AsuII | TTCGAA | 3 cut(s) | 64, 521, 759 |
| AvaI | CYCGRG | 2 cut(s) | 719, 1556 |
| AvaII | GGWCC | 4 cut(s) | 390, 564, 973, 1134 |
| AxyI | CCTNAGG | 1 cut(s) | 209 |
| BaeGI | GKGCMC | 1 cut(s) | 1056 |
| BamHI | GGATCC | 1 cut(s) | 433 |
| BanI | GGYRCC | 1 cut(s) | 1036 |
| BbsI | GAAGAC | 2 cut(s) | 779, 921 |
| Bbv12I | GWGCWC | 2 cut(s) | 546, 640 |
| BbvCI | CCTCAGC | 1 cut(s) | 125 |
| BccI | CCATC | 7 cut(s) | 133, 394, 1307, 1363, 1501, 1706, 1912 |
| BciT130I | CCWGG | 5 cut(s) | 196, 298, 438, 1071, 1132 |
| BcoDI | GTCTC | 2 cut(s) | 914, 1308 |
| BfaI | CTAG | 4 cut(s) | 408, 416, 486, 681 |
| BfmI | CTRYAG | 2 cut(s) | 1119, 1946 |
| BfoI | RGCGCY | 1 cut(s) | 1116 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 1405, 1527 |
| BglII | AGATCT | 1 cut(s) | 183 |
| BmcAI | AGTACT | 1 cut(s) | 507 |
| Bme1390I | CCNGG | 5 cut(s) | 196, 298, 438, 1071, 1132 |
| Bme18I | GGWCC | 4 cut(s) | 390, 564, 973, 1134 |
| BmeT110I | CYCGRG | 2 cut(s) | 719, 1556 |
| BmgT120I | GGNCC | 8 cut(s) | 192, 312, 330, 379, 390, 564, 973, 1134 |
| BmiI | GGNNCC | 4 cut(s) | 25, 193, 435, 1038 |
| BmrFI | CCNGG | 5 cut(s) | 196, 298, 438, 1071, 1132 |
| BmrI | ACTGGG | 2 cut(s) | 1371, 1719 |
| BmsI | GCATC | 2 cut(s) | 1255, 1877 |
| BmuI | ACTGGG | 2 cut(s) | 1371, 1719 |
| BoxI | GACNNNNGTC | 1 cut(s) | 906 |
| BpiI | GAAGAC | 2 cut(s) | 779, 921 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1153, 1185 |
| BpmI | CTGGAG | 2 cut(s) | 459, 1557 |
| Bpu10I | CCTNAGC | 2 cut(s) | 125, 893 |
| Bpu14I | TTCGAA | 3 cut(s) | 64, 521, 759 |
| BpuEI | CTTGAG | 3 cut(s) | 304, 1179, 1720 |
| BsaAI | YACGTR | 1 cut(s) | 1915 |
| BsaJI | CCNNGG | 8 cut(s) | 91, 195, 584, 1032, 1070, 1349, 1557, 1655 |
| BsaWI | WCCGGW | 1 cut(s) | 1228 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1099, 1129 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 97, 187, 264, 284, 387, 1143, 1661 |
| Bse1I | ACTGG | 6 cut(s) | 258, 551, 851, 1366, 1725, 1958 |
| Bse21I | CCTNAGG | 1 cut(s) | 209 |
| Bse3DI | GCAATG | 1 cut(s) | 1820 |
| BseAI | TCCGGA | 1 cut(s) | 1228 |
| BseBI | CCWGG | 5 cut(s) | 196, 298, 438, 1071, 1132 |
| BseDI | CCNNGG | 8 cut(s) | 91, 195, 584, 1032, 1070, 1349, 1557, 1655 |
| BseGI | GGATG | 2 cut(s) | 555, 1813 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 97, 187, 264, 284, 387, 1143, 1661 |
| BseMI | GCAATG | 1 cut(s) | 1820 |
| BseMII | CTCAG | 5 cut(s) | 116, 907, 1239, 1284, 1319 |
| BseNI | ACTGG | 6 cut(s) | 258, 551, 851, 1366, 1725, 1958 |
| BsePI | GCGCGC | 1 cut(s) | 203 |
| BseRI | GAGGAG | 1 cut(s) | 1574 |
| BseSI | GKGCMC | 1 cut(s) | 1056 |
| BsgI | GTGCAG | 2 cut(s) | 267, 1473 |
| Bsh1236I | CGCG | 1 cut(s) | 205 |
| BshFI | GGCC | 6 cut(s) | 194, 313, 331, 380, 1521, 1530 |
| BshNI | GGYRCC | 1 cut(s) | 1036 |
| BsiHKAI | GWGCWC | 2 cut(s) | 546, 640 |
| BsiHKCI | CYCGRG | 2 cut(s) | 719, 1556 |
| BsiSI | CCGG | 1 cut(s) | 1229 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 97, 187, 264, 284, 387, 1143, 1661 |
| BsmAI | GTCTC | 2 cut(s) | 914, 1308 |
| BsmI | GAATGC | 1 cut(s) | 1291 |
| BsnI | GGCC | 6 cut(s) | 194, 313, 331, 380, 1521, 1530 |
| BsoBI | CYCGRG | 2 cut(s) | 719, 1556 |
| Bsp119I | TTCGAA | 3 cut(s) | 64, 521, 759 |
| Bsp1286I | GDGCHC | 3 cut(s) | 546, 640, 1056 |
| Bsp13I | TCCGGA | 1 cut(s) | 1228 |
| Bsp143I | GATC | 4 cut(s) | 183, 335, 433, 447 |
| Bsp19I | CCATGG | 2 cut(s) | 91, 1655 |
| BspANI | GGCC | 6 cut(s) | 194, 313, 331, 380, 1521, 1530 |
| BspCNI | CTCAG | 5 cut(s) | 117, 906, 1240, 1285, 1318 |
| BspEI | TCCGGA | 1 cut(s) | 1228 |
| BspFNI | CGCG | 1 cut(s) | 205 |
| BspLI | GGNNCC | 4 cut(s) | 25, 193, 435, 1038 |
| BspPI | GGATC | 3 cut(s) | 428, 441, 442 |
| BspQI | GCTCTTC | 4 cut(s) | 938, 1176, 1194, 1434 |
| BspT104I | TTCGAA | 3 cut(s) | 64, 521, 759 |
| BspT107I | GGYRCC | 1 cut(s) | 1036 |
| BsrDI | GCAATG | 1 cut(s) | 1820 |
| BsrI | ACTGG | 6 cut(s) | 258, 551, 851, 1366, 1725, 1958 |
| BssECI | CCNNGG | 8 cut(s) | 91, 195, 584, 1032, 1070, 1349, 1557, 1655 |
| BssHII | GCGCGC | 1 cut(s) | 203 |
| BssMI | GATC | 4 cut(s) | 183, 335, 433, 447 |
| BssT1I | CCWWGG | 4 cut(s) | 91, 1032, 1349, 1655 |
| Bst2UI | CCWGG | 5 cut(s) | 196, 298, 438, 1071, 1132 |
| Bst4CI | ACNGT | 4 cut(s) | 154, 585, 1123, 1934 |
| Bst6I | CTCTTC | 5 cut(s) | 938, 1102, 1176, 1194, 1434 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 735, 807, 1274 |
| BstBAI | YACGTR | 1 cut(s) | 1915 |
| BstBI | TTCGAA | 3 cut(s) | 64, 521, 759 |
| BstC8I | GCNNGC | 2 cut(s) | 205, 727 |
| BstDEI | CTNAG | 7 cut(s) | 125, 209, 616, 893, 1248, 1293, 1305 |
| BstDSI | CCRYGG | 3 cut(s) | 91, 584, 1655 |
| BstEII | GGTNACC | 1 cut(s) | 253 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1141 |
| BstF5I | GGATG | 2 cut(s) | 555, 1813 |
| BstFNI | CGCG | 1 cut(s) | 205 |
| BstH2I | RGCGCY | 1 cut(s) | 1116 |
| BstHHI | GCGC | 3 cut(s) | 205, 207, 1115 |
| BstKTI | GATC | 4 cut(s) | 186, 338, 436, 450 |
| BstMAI | GTCTC | 2 cut(s) | 914, 1308 |
| BstMBI | GATC | 4 cut(s) | 183, 335, 433, 447 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 200, 735, 744, 807, 968, 1274, 1405, 1527 |
| BstNI | CCWGG | 5 cut(s) | 196, 298, 438, 1071, 1132 |
| BstNSI | RCATGY | 3 cut(s) | 729, 738, 1277 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 906 |
| BstPI | GGTNACC | 1 cut(s) | 253 |
| BstSCI | CCNGG | 5 cut(s) | 194, 296, 436, 1069, 1130 |
| BstSFI | CTRYAG | 2 cut(s) | 1119, 1946 |
| BstSLI | GKGCMC | 1 cut(s) | 1056 |
| BstUI | CGCG | 1 cut(s) | 205 |
| BstV2I | GAAGAC | 2 cut(s) | 779, 921 |
| BstX2I | RGATCY | 2 cut(s) | 183, 433 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 400 |
| BstYI | RGATCY | 2 cut(s) | 183, 433 |
| Bsu36I | CCTNAGG | 1 cut(s) | 209 |
| BsuRI | GGCC | 6 cut(s) | 194, 313, 331, 380, 1521, 1530 |
| BtgI | CCRYGG | 3 cut(s) | 91, 584, 1655 |
| BtsCI | GGATG | 2 cut(s) | 555, 1813 |
| BtsIMutI | CAGTG | 5 cut(s) | 265, 544, 655, 1509, 1951 |
| Cac8I | GCNNGC | 2 cut(s) | 205, 727 |
| CfoI | GCGC | 3 cut(s) | 205, 207, 1115 |
| Cfr13I | GGNCC | 8 cut(s) | 192, 312, 330, 379, 390, 564, 973, 1134 |
| CpoI | CGGWCCG | 1 cut(s) | 973 |
| CseI | GACGC | 1 cut(s) | 1604 |
| CsiI | ACCWGGT | 1 cut(s) | 1130 |
| Csp6I | GTAC | 4 cut(s) | 39, 506, 590, 1912 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 264, 268, 299, 303, 1334, 1369 |
| CspI | CGGWCCG | 1 cut(s) | 973 |
| CviQI | GTAC | 4 cut(s) | 39, 506, 590, 1912 |
| DdeI | CTNAG | 7 cut(s) | 125, 209, 616, 893, 1248, 1293, 1305 |
| DpnI | GATC | 4 cut(s) | 185, 337, 435, 449 |
| DpnII | GATC | 4 cut(s) | 183, 335, 433, 447 |
| DraI | TTTAAA | 1 cut(s) | 1624 |
| EaeI | YGGCCR | 1 cut(s) | 1528 |
| Eam1104I | CTCTTC | 5 cut(s) | 938, 1102, 1176, 1194, 1434 |
| EarI | CTCTTC | 5 cut(s) | 938, 1102, 1176, 1194, 1434 |
| EciI | GGCGGA | 2 cut(s) | 185, 1793 |
| Eco130I | CCWWGG | 4 cut(s) | 91, 1032, 1349, 1655 |
| Eco47I | GGWCC | 4 cut(s) | 390, 564, 973, 1134 |
| Eco47III | AGCGCT | 1 cut(s) | 1114 |
| Eco57I | CTGAAG | 2 cut(s) | 999, 1062 |
| Eco81I | CCTNAGG | 1 cut(s) | 209 |
| Eco88I | CYCGRG | 2 cut(s) | 719, 1556 |
| Eco91I | GGTNACC | 1 cut(s) | 253 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1141 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1134 |
| EcoO65I | GGTNACC | 1 cut(s) | 253 |
| EcoRII | CCWGG | 5 cut(s) | 194, 296, 436, 1069, 1130 |
| EcoT14I | CCWWGG | 4 cut(s) | 91, 1032, 1349, 1655 |
| EcoT22I | ATGCAT | 1 cut(s) | 731 |
| ErhI | CCWWGG | 4 cut(s) | 91, 1032, 1349, 1655 |
| FauI | CCCGC | 1 cut(s) | 89 |
| FokI | GGATG | 2 cut(s) | 562, 1800 |
| FspBI | CTAG | 4 cut(s) | 408, 416, 486, 681 |
| GlaI | GCGC | 3 cut(s) | 204, 206, 1114 |
| GsuI | CTGGAG | 2 cut(s) | 459, 1557 |
| HaeII | RGCGCY | 1 cut(s) | 1116 |
| HaeIII | GGCC | 6 cut(s) | 194, 313, 331, 380, 1521, 1530 |
| HapII | CCGG | 1 cut(s) | 1229 |
| HgaI | GACGC | 1 cut(s) | 1604 |
| HhaI | GCGC | 3 cut(s) | 205, 207, 1115 |
| Hin6I | GCGC | 3 cut(s) | 203, 205, 1113 |
| HinP1I | GCGC | 3 cut(s) | 203, 205, 1113 |
| HindIII | AAGCTT | 1 cut(s) | 1647 |
| HinfI | GANTC | 9 cut(s) | 373, 404, 1180, 1192, 1478, 1704, 1762, 1789, 1883 |
| HpaII | CCGG | 1 cut(s) | 1229 |
| HphI | GGTGA | 4 cut(s) | 265, 491, 1487, 1762 |
| Hpy166II | GTNNAC | 4 cut(s) | 390, 564, 1210, 1356 |
| Hpy188I | TCNGA | 8 cut(s) | 327, 781, 1000, 1018, 1249, 1308, 1552, 1594 |
| Hpy188III | TCNNGA | 7 cut(s) | 498, 911, 942, 1175, 1196, 1229, 1457 |
| Hpy8I | GTNNAC | 4 cut(s) | 390, 564, 1210, 1356 |
| Hpy99I | CGWCG | 1 cut(s) | 118 |
| HpyAV | CCTTC | 7 cut(s) | 339, 624, 948, 1023, 1601, 1655, 1698 |
| HpyCH4III | ACNGT | 4 cut(s) | 154, 585, 1123, 1934 |
| HpyCH4IV | ACGT | 3 cut(s) | 37, 1525, 1914 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 200, 735, 744, 807, 968, 1274, 1405, 1527 |
| HpyF3I | CTNAG | 7 cut(s) | 125, 209, 616, 893, 1248, 1293, 1305 |
| HpySE526I | ACGT | 3 cut(s) | 37, 1525, 1914 |
| HspAI | GCGC | 3 cut(s) | 203, 205, 1113 |
| Kpn2I | TCCGGA | 1 cut(s) | 1228 |
| Kzo9I | GATC | 4 cut(s) | 183, 335, 433, 447 |
| LguI | GCTCTTC | 4 cut(s) | 938, 1176, 1194, 1434 |
| LmnI | GCTCC | 4 cut(s) | 268, 541, 883, 1120 |
| LweI | GCATC | 2 cut(s) | 1255, 1877 |
| MabI | ACCWGGT | 1 cut(s) | 1130 |
| MaeI | CTAG | 4 cut(s) | 408, 416, 486, 681 |
| MaeII | ACGT | 3 cut(s) | 37, 1525, 1914 |
| MalI | GATC | 4 cut(s) | 185, 337, 435, 449 |
| MboI | GATC | 4 cut(s) | 183, 335, 433, 447 |
| MflI | RGATCY | 2 cut(s) | 183, 433 |
| MhlI | GDGCHC | 3 cut(s) | 546, 640, 1056 |
| MluCI | AATT | 5 cut(s) | 66, 280, 362, 457, 1298 |
| MlyI | GAGTC | 4 cut(s) | 398, 1186, 1189, 1713 |
| MmeI | TCCRAC | 4 cut(s) | 85, 196, 322, 1623 |
| Mph1103I | ATGCAT | 1 cut(s) | 731 |
| MroI | TCCGGA | 1 cut(s) | 1228 |
| MroXI | GAANNNNTTC | 5 cut(s) | 786, 932, 1162, 1224, 1443 |
| MseI | TTAA | 6 cut(s) | 705, 1047, 1239, 1257, 1565, 1623 |
| MslI | CAYNNNNRTG | 2 cut(s) | 583, 1215 |
| MspA1I | CMGCKG | 5 cut(s) | 164, 251, 627, 971, 1828 |
| MspI | CCGG | 1 cut(s) | 1229 |
| MspR9I | CCNGG | 5 cut(s) | 196, 298, 438, 1071, 1132 |
| Mva1269I | GAATGC | 1 cut(s) | 1291 |
| MvaI | CCWGG | 5 cut(s) | 196, 298, 438, 1071, 1132 |
| MvnI | CGCG | 1 cut(s) | 205 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 200, 735, 744, 807, 968, 1274, 1405, 1527 |
| NcoI | CCATGG | 2 cut(s) | 91, 1655 |
| NdeII | GATC | 4 cut(s) | 183, 335, 433, 447 |
| NlaIV | GGNNCC | 4 cut(s) | 25, 193, 435, 1038 |
| NmeAIII | GCCGAG | 1 cut(s) | 1880 |
| NmuCI | GTSAC | 4 cut(s) | 133, 154, 253, 300 |
| NsiI | ATGCAT | 1 cut(s) | 731 |
| NspI | RCATGY | 3 cut(s) | 729, 738, 1277 |
| NspV | TTCGAA | 3 cut(s) | 64, 521, 759 |
| OliI | CACNNNNGTG | 1 cut(s) | 583 |
| PaeI | GCATGC | 1 cut(s) | 729 |
| PaeR7I | CTCGAG | 1 cut(s) | 719 |
| PauI | GCGCGC | 1 cut(s) | 203 |
| PciSI | GCTCTTC | 4 cut(s) | 938, 1176, 1194, 1434 |
| PctI | GAATGC | 1 cut(s) | 1291 |
| PdmI | GAANNNNTTC | 5 cut(s) | 786, 932, 1162, 1224, 1443 |
| PfeI | GAWTC | 5 cut(s) | 373, 1478, 1762, 1789, 1883 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 187 |
| PfoI | TCCNGGA | 1 cut(s) | 436 |
| PleI | GAGTC | 4 cut(s) | 398, 1186, 1188, 1712 |
| PpsI | GAGTC | 4 cut(s) | 398, 1186, 1188, 1712 |
| Ppu21I | YACGTR | 1 cut(s) | 1915 |
| PpuMI | RGGWCCY | 1 cut(s) | 1134 |
| PshAI | GACNNNNGTC | 1 cut(s) | 906 |
| PsiI | TTATAA | 1 cut(s) | 1925 |
| Psp5II | RGGWCCY | 1 cut(s) | 1134 |
| Psp6I | CCWGG | 5 cut(s) | 194, 296, 436, 1069, 1130 |
| PspEI | GGTNACC | 1 cut(s) | 253 |
| PspGI | CCWGG | 5 cut(s) | 194, 296, 436, 1069, 1130 |
| PspN4I | GGNNCC | 4 cut(s) | 25, 193, 435, 1038 |
| PspPI | GGNCC | 8 cut(s) | 192, 312, 330, 379, 390, 564, 973, 1134 |
| PspPPI | RGGWCCY | 1 cut(s) | 1134 |
| PspXI | VCTCGAGB | 1 cut(s) | 719 |
| PsuI | RGATCY | 2 cut(s) | 183, 433 |
| PteI | GCGCGC | 1 cut(s) | 203 |
| PvuII | CAGCTG | 1 cut(s) | 1828 |
| RsaI | GTAC | 4 cut(s) | 40, 507, 591, 1913 |
| RsaNI | GTAC | 4 cut(s) | 39, 506, 590, 1912 |
| RseI | CAYNNNNRTG | 2 cut(s) | 583, 1215 |
| Rsr2I | CGGWCCG | 1 cut(s) | 973 |
| RsrII | CGGWCCG | 1 cut(s) | 973 |
| SapI | GCTCTTC | 4 cut(s) | 938, 1176, 1194, 1434 |
| SaqAI | TTAA | 6 cut(s) | 705, 1047, 1239, 1257, 1565, 1623 |
| Sau3AI | GATC | 4 cut(s) | 183, 335, 433, 447 |
| Sau96I | GGNCC | 8 cut(s) | 192, 312, 330, 379, 390, 564, 973, 1134 |
| ScaI | AGTACT | 1 cut(s) | 507 |
| SchI | GAGTC | 4 cut(s) | 398, 1186, 1189, 1713 |
| ScrFI | CCNGG | 5 cut(s) | 196, 298, 438, 1071, 1132 |
| SduI | GDGCHC | 3 cut(s) | 546, 640, 1056 |
| SexAI | ACCWGGT | 1 cut(s) | 1130 |
| SfaNI | GCATC | 2 cut(s) | 1255, 1877 |
| SfcI | CTRYAG | 2 cut(s) | 1119, 1946 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 1527 |
| Sfr274I | CTCGAG | 1 cut(s) | 719 |
| SfuI | TTCGAA | 3 cut(s) | 64, 521, 759 |
| SinI | GGWCC | 4 cut(s) | 390, 564, 973, 1134 |
| SlaI | CTCGAG | 1 cut(s) | 719 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 583, 1215 |
| SmlI | CTYRAG | 4 cut(s) | 319, 719, 1194, 1699 |
| SmoI | CTYRAG | 4 cut(s) | 319, 719, 1194, 1699 |
| SphI | GCATGC | 1 cut(s) | 729 |
| Sse9I | AATT | 5 cut(s) | 66, 280, 362, 457, 1298 |
| SspMI | CTAG | 4 cut(s) | 408, 416, 486, 681 |
| StyD4I | CCNGG | 5 cut(s) | 194, 296, 436, 1069, 1130 |
| StyI | CCWWGG | 4 cut(s) | 91, 1032, 1349, 1655 |
| TaaI | ACNGT | 4 cut(s) | 154, 585, 1123, 1934 |
| TaiI | ACGT | 3 cut(s) | 40, 1528, 1917 |
| TaqI | TCGA | 8 cut(s) | 64, 308, 521, 720, 759, 912, 1190, 1936 |
| TasI | AATT | 5 cut(s) | 66, 280, 362, 457, 1298 |
| TatI | WGTACW | 1 cut(s) | 505 |
| TauI | GCSGC | 3 cut(s) | 167, 170, 1533 |
| TfiI | GAWTC | 5 cut(s) | 373, 1478, 1762, 1789, 1883 |
| Tru1I | TTAA | 6 cut(s) | 705, 1047, 1239, 1257, 1565, 1623 |
| Tru9I | TTAA | 6 cut(s) | 705, 1047, 1239, 1257, 1565, 1623 |
| TscAI | CASTG | 5 cut(s) | 265, 551, 655, 1509, 1958 |
| TseFI | GTSAC | 4 cut(s) | 133, 154, 253, 300 |
| Tsp45I | GTSAC | 4 cut(s) | 133, 154, 253, 300 |
| TspDTI | ATGAA | 7 cut(s) | 450, 1089, 1233, 1446, 1620, 1680, 1723 |
| TspRI | CASTG | 5 cut(s) | 265, 551, 655, 1509, 1958 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 187 |
| VpaK11BI | GGWCC | 4 cut(s) | 390, 564, 973, 1134 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1141 |
| XapI | RAATTY | 1 cut(s) | 457 |
| XceI | RCATGY | 3 cut(s) | 729, 738, 1277 |
| XhoI | CTCGAG | 1 cut(s) | 719 |
| XmnI | GAANNNNTTC | 5 cut(s) | 786, 932, 1162, 1224, 1443 |
| XspI | CTAG | 4 cut(s) | 408, 416, 486, 681 |
| ZrmI | AGTACT | 1 cut(s) | 507 |
| Zsp2I | ATGCAT | 1 cut(s) | 731 |