Rorug02G0232700

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000152
Physical Location & Seq
Reverse (-)
23241697 .. 23246596
4900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0232700.1

Sequence Viewer

Length: 2334 bp
ATGTTCAGTAAGAAACCCATCCACCTCTTCAAACGCTTTCCCTCCTCTTTTCCATTCTCTTCACTTTCACCTTCCAACTACCTTAACTGTTATCTCCAATCTCTCCTCTCCAACCAAACTTCAACTCTCCCACACCTCTCTCAATCCCACGCTCTCATTATCACTTCTGGAAACTCAAACAACGTTTTCATAGCCTCAAAGCTCGTCTCTTTCTATGCCTCTCTCACCAAACCCGAATCTTCCACCAAAGTATTTGATTCAGTTTCTCCCAAAGACACATTCCTCTGGAACTCCATAATCAAAACCCACTTCTCCAATGGCAGTTACTCCAATGCCCTCGAGTTCTTTACCCAAATGCGAGGCTCCGAGTTGGGTCCCAACCAATTCACTCTTCCAATGGTTGTTGCTTCCTGCGCCGAGTTGAGGTTGCTAGACCATGGCAAGACTGTTCATGGGCTGGCTTCAAAACTCGGCCTTTTCGCGGGGAATCCCGCAGTTGGGTCATCTTTTGTCTACATGTACTCGAAATGCGGTCGAATGGGGGATGCATGTGTCGTGTTCGATGAAATTACTGAGAGAGATGTGGTGTGTTGGACTGCGGTTATAATTGGGTATGTGCAGAATGGTGAGAGTGAGAAGGGTTTGGAGTGTCTTTGTGAGATGCATAGGATTGGTGGAGATGGTGAGAGGCCGAATTTTAGGACACTGGAAGTTGGGTTTCAAGCTTGTGTGGGTATGGGAGCTATAGGAGAAGGTAGATGTTTACATGGTTTTGTTGTGAAAAGTGGAATTGGATGTTCAGAAGCTGTCAAGTCTTTGATTTTGTCTATGTATTCCAGATGTGGGACACCTGGTGAATCCTTCGTTTTGTTTCGTGAATTGAGAAATAGAGATCTTCTATCATGGACGTCGGTTATTGGTGCTTATACTAGATCTGGGTTAGTGGCAGAATGCTTGTGTTTATTTTGGGAAATGCAGGGTAGTGATATTGTTGCGGATGAAATTGTTGTCAGTTGCATGCTTTCAGGGTTTAGAAATTTGACAAATATCAGGGAAGGAAAGGCATTCCTTGGAATAGTGACAAGGCAAAACTATGGATCAACTCAGATGGTTTATAGTGAACTGGTATCCATGTACTGCAAGTTTGGGTTATTGACTCTTGCAGAGAAGCTTCTTAGTGAAATGCAGCATTGGGACAAAGAGTGTTGTAATAATATGATTTGTGGCTATGCTAAGGTGGGACAGCCTGCAAAATGTATAGAAATTTTTGGGAAGATGCAATATTTAGGCATTGAAGCTGATTCTAGTAGCTTGGTGTCTGTGATTTGTTCATGCTTCCAGTTGGGGGCGATCCATCTTGGCCAATCAGTTCATGGCTACATGATTAAGATTTCGATGGATGACAATGTCTCGGTAACCAATTCACTCATAGATATGTATGGAAAAAGTGGTTATTTGAACATTGCATTGAGAATTTTTCGTGGGACACATGGAGACATTGTAACATGGAACACAATGATGTCATCTTACACTCACAATGGAAATTTTGCTGAGGCCATAGTCCTATTTCATAAAATGACAGCAGAGAAGGTGAAGCCCAACTCGGCAACATTTGTAACGGTGCTTTCTGCTTGCTCTCATCTCGCATCTCTTAAAGAAGGAGAGAAAGTTCACTGTCACATTAAGGAAAGAGGGATAGAGATCAATTTTTCCGTTGCCGCTGCATTGGTTGATATGTATGCAAAATGTGGGCAGCTCAAGAAATCTAGAGAACTGTTCAACTCAATGAATGAGAGGGATGCTATTTCTTGGAATGTCATGATCTCAGGTTATGCTATGCACGGACATGCAGAATCTGCAATAGAAATTTTCCACAACATGGAAACTGCAAATGTTAAACCAAATGAAATAACATTCCTTTCTCTTCTTTCAGCCTGTAATCATTCAGGGCTTGTTGAACAAGGGAAGTATCTGTTTGGTAGAATGCAGGAATTTTCTGTAAAGCCCAATTTGAAGCACTATGCTTGTATGGTAGATATTCTTGGAAGGTCTGGTAAACTGCAAGAAGCTAAACATTTGGTTCTGTCAATGCCCATTACTCCTGATGGTGGTGTATGGGGATCTCTGTTAGCTGCCTGTAAGATTCACAATGAAATTGAAATGGGTGCAAGGGTTGCTAAGCATGCTATAGAATCTGATCCAGAAAATGACGGCTATTATATAATGCTTTCCAACCTGTATAGTTCCATTGGAAGGTGGGATGATGCTATAAGTGTGAGAAAAATGATGCAGGAGAAGGGTGTGGGAAAGACAAATGCATGGAGTGTGGTGTAA

Protein Analysis

777

Amino Acids

85.69

Weight (kDa)

7.28

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 499 - 546 4.7e-13 PPR repeat family
PPR PF01535 501 - 529 6.8e-07 PPR repeat
PPR_2 PF13041 600 - 646 4.8e-12 PPR repeat family
PPR PF01535 602 - 631 1.9e-06 PPR repeat
PPR_3 PF13812 623 - 681 3.3e-06 Pentatricopeptide repeat domain
E_motif PF20431 716 - 771 3e-11 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000152)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17950
fragaria_vesca FvH4_1g11910 FvH4_3g04400
malus_domestica MD02G1133000.v1.1 MD15G1245600.v1.1
prunus_persica Prupe.7G167700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0314921 RchiOBHm_Chr1g0319941 RchiOBHm_Chr1g0321251 RchiOBHm_Chr1g0323021 RchiOBHm_Chr1g0324491 RchiOBHm_Chr1g0325171 RchiOBHm_Chr1g0360601 RchiOBHm_Chr1g0365451 RchiOBHm_Chr1g0381201 RchiOBHm_Chr2g0085401 RchiOBHm_Chr2g0099501 RchiOBHm_Chr2g0106981 RchiOBHm_Chr2g0130271 RchiOBHm_Chr2g0155121 RchiOBHm_Chr3g0455971 RchiOBHm_Chr3g0459501 RchiOBHm_Chr3g0478111 RchiOBHm_Chr3g0480661 RchiOBHm_Chr4g0388561 RchiOBHm_Chr4g0394421 RchiOBHm_Chr4g0424371 RchiOBHm_Chr5g0028631 RchiOBHm_Chr5g0069751 RchiOBHm_Chr5g0076691 RchiOBHm_Chr5g0076711 RchiOBHm_Chr5g0076721 RchiOBHm_Chr5g0079671 RchiOBHm_Chr5g0079731 RchiOBHm_Chr5g0079911 RchiOBHm_Chr5g0081741 RchiOBHm_Chr6g0255231 RchiOBHm_Chr6g0283251 RchiOBHm_Chr6g0300091 RchiOBHm_Chr7g0190751 RchiOBHm_Chr7g0240241
rosa_multiflora Rmu_co8441375.1_g000001 Rmu_sc0000110.1_g000027 Rmu_sc0000187.1_g000008 Rmu_sc0000240.1_g000065 Rmu_sc0000251.1_g000004 Rmu_sc0000272.1_g000025 Rmu_sc0000276.1_g000074 Rmu_sc0000284.1_g000010 Rmu_sc0000285.1_g000021 Rmu_sc0000360.1_g000034 Rmu_sc0000365.1_g000038 Rmu_sc0000368.1_g000013 Rmu_sc0000368.1_g000025 Rmu_sc0000770.1_g000044 Rmu_sc0000770.1_g000052 Rmu_sc0000795.1_g000098 Rmu_sc0000945.1_g000037 Rmu_sc0000949.1_g000023 Rmu_sc0000982.1_g000002 Rmu_sc0001424.1_g000004 Rmu_sc0001775.1_g000004 Rmu_sc0001952.1_g000010 Rmu_sc0002080.1_g000046 Rmu_sc0002141.1_g000016 Rmu_sc0002183.1_g000004 Rmu_sc0002187.1_g000040 Rmu_sc0002263.1_g000057 Rmu_sc0002351.1_g000061 Rmu_sc0002542.1_g000017 Rmu_sc0002751.1_g000003 Rmu_sc0002792.1_g000029 Rmu_sc0002798.1_g000020 Rmu_sc0002985.1_g000010 Rmu_sc0003124.1_g000019 Rmu_sc0003207.1_g000019 Rmu_sc0003237.1_g000003 Rmu_sc0003363.1_g000050 Rmu_sc0003443.1_g000025 Rmu_sc0003465.1_g000032 Rmu_sc0003500.1_g000012 Rmu_sc0003809.1_g000026 Rmu_sc0003859.1_g000032 Rmu_sc0004085.1_g000003 Rmu_sc0004085.1_g000008 Rmu_sc0004571.1_g000019 Rmu_sc0004753.1_g000006 Rmu_sc0004865.1_g000017 Rmu_sc0004881.1_g000007 Rmu_sc0005697.1_g000023 Rmu_sc0005812.1_g000020 Rmu_sc0007076.1_g000001 Rmu_sc0007801.1_g000003 Rmu_sc0008355.1_g000007 Rmu_sc0009380.1_g000006 Rmu_sc0010191.1_g000004 Rmu_sc0010252.1_g000006 Rmu_sc0011026.1_g000001 Rmu_sc0011824.1_g000003 Rmu_sc0012533.1_g000006 Rmu_sc0012640.1_g000006 Rmu_sc0014032.1_g000001 Rmu_sc0014545.1_g000001 Rmu_sc0014840.1_g000008 Rmu_sc0015060.1_g000013 Rmu_sc0018122.1_g000003 Rmu_sc0026563.1_g000004 Rmu_ssc0000213.1_g000057 Rmu_ssc0000213.1_g000091 Rmu_ssc0000262.1_g000021 Rmu_ssc0000282.1_g000021 Rmu_ssc0000295.1_g000041 Rmu_ssc0000438.1_g000002 Rmu_ssc0000472.1_g000015
rosa_roxburghii Rroxscaffold_1G00020080 Rroxscaffold_2G00084980 Rroxscaffold_2G00143020 Rroxscaffold_3G00261750 Rroxscaffold_3G00268610 Rroxscaffold_4G00312950 Rroxscaffold_7G00156650 Rroxscaffold_7G00215160
rosa_rugosa Rorug01G0077100 Rorug01G0201100 Rorug01G0246900 Rorug01G0256600 Rorug02G0080200 Rorug02G0089300 Rorug02G0090700 Rorug02G0177200 Rorug02G0187000 Rorug02G0192600 Rorug02G0232700 Rorug02G0262100 Rorug02G0328300 Rorug02G0408300 Rorug02G0641200 Rorug03G0155000 Rorug03G0185500 Rorug03G0256500 Rorug03G0291000 Rorug03G0299400 Rorug03G0317000 Rorug03G0326700 Rorug03G0330800 Rorug03G0339700 Rorug03G0352900 Rorug04G0029800 Rorug04G0029900 Rorug04G0048100 Rorug04G0075800 Rorug04G0124400 Rorug04G0212100 Rorug04G0212400 Rorug04G0253200 Rorug05G0116700 Rorug05G0146500 Rorug05G0211900 Rorug05G0215700 Rorug05G0216100 Rorug05G0220500 Rorug05G0339400 Rorug05G0352000 Rorug05G0356500.1 Rorug05G0398900 Rorug05G0474700 Rorug05G0511400 Rorug05G0540200 Rorug05G0541000 Rorug05G0572900.1 Rorug05G0584000 Rorug06G0000900 Rorug06G0014600 Rorug06G0018800 Rorug06G0026100 Rorug06G0042900 Rorug06G0082600 Rorug06G0133900 Rorug06G0229100 Rorug07G0321400 Rorug07G0326400 Rorug07G0326600.1
rosa_wichuraiana Rw0G017710 Rw1G012170 Rw1G039350 Rw2G010030 Rw3G024430 Rw5G045720 Rw7G025340 Rw7G040870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
AatII GACGTC 1 cut(s) 911
AccB7I CCANNNNNTGG 1 cut(s) 1879
AccI GTMKAC 1 cut(s) 513
AccII CGCG 1 cut(s) 482
AciI CCGC 6 cut(s) 482, 492, 531, 599, 995, 1719
AclI AACGTT 1 cut(s) 183
AclWI GGATC 4 cut(s) 1105, 1345, 2128, 2192
AcoI YGGCCR 1 cut(s) 1360
AcsI RAATTY 7 cut(s) 694, 1036, 1263, 1473, 1543, 1866, 1991
AcyI GRCGYC 1 cut(s) 908
AdeI CACNNNGTG 1 cut(s) 854
AfaI GTAC 2 cut(s) 521, 1136
AfiI CCNNNNNNNGG 9 cut(s) 423, 481, 497, 498, 843, 1345, 1879, 2108, 2253
AflIII ACRYGT 1 cut(s) 516
AjnI CCWGG 1 cut(s) 850
AjuI GAANNNNNNNTTGG 4 cut(s) 263, 295, 375, 407
AloI GAACNNNNNNTCC 2 cut(s) 1653, 1685
Alw26I GTCTC 3 cut(s) 211, 1414, 1488
AlwI GGATC 4 cut(s) 1105, 1345, 2128, 2192
AlwNI CAGNNNCTG 2 cut(s) 806, 1856
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 4 cut(s) 472, 689, 1360, 1554
ApeKI GCWGC 4 cut(s) 1186, 1721, 1753, 2132
ApoI RAATTY 7 cut(s) 694, 1036, 1263, 1473, 1543, 1866, 1991
AspLEI GCGC 1 cut(s) 416
AspS9I GGNCC 1 cut(s) 374
AsuHPI GGTGA 6 cut(s) 60, 217, 638, 695, 866, 1603
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 1 cut(s) 374
BalI TGGCCA 1 cut(s) 1362
BbvCI CCTCAGC 1 cut(s) 1551
BbvI GCAGC 4 cut(s) 1198, 1708, 1765, 2119
BccI CCATC 6 cut(s) 26, 674, 1102, 1362, 1390, 2099
BceAI ACGGC 1 cut(s) 2227
BciT130I CCWGG 1 cut(s) 852
BciVI GTATCC 1 cut(s) 1138
BcoDI GTCTC 3 cut(s) 211, 1414, 1488
BfaI CTAG 4 cut(s) 431, 930, 1305, 1767
BfmI CTRYAG 2 cut(s) 744, 2187
BfuI GTATCC 1 cut(s) 1138
BglII AGATCT 2 cut(s) 892, 932
BisI GCNGC 5 cut(s) 1187, 1719, 1722, 1754, 2133
BlpI GCTNAGC 1 cut(s) 2178
BlsI GCNGC 5 cut(s) 1188, 1720, 1723, 1755, 2134
Bme1390I CCNGG 1 cut(s) 852
Bme18I GGWCC 1 cut(s) 374
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 3 cut(s) 364, 375, 376
BmrFI CCNGG 1 cut(s) 852
BmsI GCATC 7 cut(s) 535, 651, 1266, 1655, 1789, 2254, 2277
Bpu10I CCTNAGC 2 cut(s) 1233, 1551
Bpu1102I GCTNAGC 1 cut(s) 2178
BpuEI CTTGAG 1 cut(s) 1742
BsaBI GATNNNNATC 1 cut(s) 1700
BsaHI GRCGYC 1 cut(s) 908
BsaJI CCNNGG 2 cut(s) 436, 1069
BsaXI ACNNNNNCTCC 4 cut(s) 1485, 1515, 1653, 1683
Bsc4I CCNNNNNNNGG 9 cut(s) 423, 481, 497, 498, 843, 1345, 1879, 2108, 2253
Bse1I ACTGG 3 cut(s) 711, 1128, 1339
Bse3DI GCAATG 1 cut(s) 1461
Bse8I GATNNNNATC 1 cut(s) 1700
BseBI CCWGG 1 cut(s) 852
BseDI CCNNGG 2 cut(s) 436, 1069
BseGI GGATG 7 cut(s) 18, 550, 800, 1003, 1405, 1804, 2266
BseJI GATNNNNATC 1 cut(s) 1700
BseLI CCNNNNNNNGG 9 cut(s) 423, 481, 497, 498, 843, 1345, 1879, 2108, 2253
BseMI GCAATG 1 cut(s) 1461
BseMII CTCAG 4 cut(s) 564, 1118, 1542, 1839
BseNI ACTGG 3 cut(s) 711, 1128, 1339
BseRI GAGGAG 2 cut(s) 34, 95
BseXI GCAGC 4 cut(s) 1198, 1708, 1765, 2119
BsgI GTGCAG 1 cut(s) 638
Bsh1236I CGCG 1 cut(s) 482
Bsh1285I CGRYCG 1 cut(s) 535
BshFI GGCC 4 cut(s) 474, 691, 1362, 1556
BsiEI CGRYCG 1 cut(s) 535
BsiHKCI CYCGRG 1 cut(s) 338
BslFI GGGAC 5 cut(s) 360, 859, 1208, 1254, 1498
BslI CCNNNNNNNGG 9 cut(s) 423, 481, 497, 498, 843, 1345, 1879, 2108, 2253
BsmAI GTCTC 3 cut(s) 211, 1414, 1488
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 5 cut(s) 360, 859, 1208, 1254, 1498
BsmI GAATGC 3 cut(s) 956, 1064, 1989
BsnI GGCC 4 cut(s) 474, 691, 1362, 1556
BsoBI CYCGRG 1 cut(s) 338
Bsp143I GATC 8 cut(s) 892, 932, 1097, 1350, 1701, 1821, 2120, 2197
Bsp1720I GCTNAGC 1 cut(s) 2178
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 6 cut(s) 482, 492, 531, 599, 995, 1719
BspANI GGCC 4 cut(s) 474, 691, 1362, 1556
BspCNI CTCAG 4 cut(s) 565, 1117, 1543, 1838
BspFNI CGCG 1 cut(s) 482
BspHI TCATGA 1 cut(s) 1818
BspLI GGNNCC 3 cut(s) 364, 375, 376
BspPI GGATC 4 cut(s) 1105, 1345, 2128, 2192
BsrDI GCAATG 1 cut(s) 1461
BsrI ACTGG 3 cut(s) 711, 1128, 1339
BssECI CCNNGG 2 cut(s) 436, 1069
BssMI GATC 8 cut(s) 892, 932, 1097, 1350, 1701, 1821, 2120, 2197
BssNI GRCGYC 1 cut(s) 908
BssT1I CCWWGG 2 cut(s) 436, 1069
Bst2UI CCWGG 1 cut(s) 852
Bst4CI ACNGT 5 cut(s) 89, 448, 1621, 1676, 1776
Bst6I CTCTTC 4 cut(s) 32, 64, 396, 1929
BstACI GRCGYC 1 cut(s) 908
BstAPI GCANNNNNTGC 2 cut(s) 1856, 2174
BstC8I GCNNGC 5 cut(s) 459, 1019, 1248, 1633, 2184
BstDEI CTNAG 7 cut(s) 573, 1104, 1175, 1233, 1551, 1825, 2178
BstDSI CCRYGG 1 cut(s) 436
BstEII GGTNACC 1 cut(s) 1414
BstF5I GGATG 7 cut(s) 18, 550, 800, 1003, 1405, 1804, 2266
BstFNI CGCG 1 cut(s) 482
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 8 cut(s) 895, 935, 1100, 1353, 1704, 1824, 2123, 2200
BstMAI GTCTC 3 cut(s) 211, 1414, 1488
BstMBI GATC 8 cut(s) 892, 932, 1097, 1350, 1701, 1821, 2120, 2197
BstMCI CGRYCG 1 cut(s) 535
BstMWI GCNNNNNNNGC 4 cut(s) 413, 1856, 2174, 2183
BstNI CCWGG 1 cut(s) 852
BstNSI RCATGY 5 cut(s) 520, 552, 1021, 1850, 2186
BstPI GGTNACC 1 cut(s) 1414
BstSCI CCNGG 1 cut(s) 850
BstSFI CTRYAG 2 cut(s) 744, 2187
BstUI CGCG 1 cut(s) 482
BstV1I GCAGC 4 cut(s) 1198, 1708, 1765, 2119
BstX2I RGATCY 3 cut(s) 892, 932, 2120
BstYI RGATCY 3 cut(s) 892, 932, 2120
BsuI GTATCC 1 cut(s) 1138
BsuRI GGCC 4 cut(s) 474, 691, 1362, 1556
BtgI CCRYGG 1 cut(s) 436
BtsCI GGATG 7 cut(s) 18, 550, 800, 1003, 1405, 1804, 2266
BtsIMutI CAGTG 2 cut(s) 704, 1672
Cac8I GCNNGC 5 cut(s) 459, 1019, 1248, 1633, 2184
CaiI CAGNNNCTG 2 cut(s) 806, 1856
CciI TCATGA 1 cut(s) 1818
CfoI GCGC 1 cut(s) 416
Cfr13I GGNCC 1 cut(s) 374
CsiI ACCWGGT 1 cut(s) 850
Csp6I GTAC 2 cut(s) 520, 1135
CviQI GTAC 2 cut(s) 520, 1135
DdeI CTNAG 7 cut(s) 573, 1104, 1175, 1233, 1551, 1825, 2178
DpnI GATC 8 cut(s) 894, 934, 1099, 1352, 1703, 1823, 2122, 2199
DpnII GATC 8 cut(s) 892, 932, 1097, 1350, 1701, 1821, 2120, 2197
DraIII CACNNNGTG 1 cut(s) 854
EaeI YGGCCR 1 cut(s) 1360
Eam1104I CTCTTC 4 cut(s) 32, 64, 396, 1929
EarI CTCTTC 4 cut(s) 32, 64, 396, 1929
Eco130I CCWWGG 2 cut(s) 436, 1069
Eco47I GGWCC 1 cut(s) 374
Eco88I CYCGRG 1 cut(s) 338
Eco91I GGTNACC 1 cut(s) 1414
EcoO109I RGGNCCY 1 cut(s) 374
EcoO65I GGTNACC 1 cut(s) 1414
EcoRII CCWGG 1 cut(s) 850
EcoT14I CCWWGG 2 cut(s) 436, 1069
EcoT22I ATGCAT 3 cut(s) 550, 666, 2320
ErhI CCWWGG 2 cut(s) 436, 1069
Esp3I CGTCTC 1 cut(s) 211
FaqI GGGAC 5 cut(s) 360, 859, 1208, 1254, 1498
FauI CCCGC 2 cut(s) 475, 499
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 5 cut(s) 1187, 1719, 1722, 1754, 2133
FokI GGATG 7 cut(s) 5, 557, 807, 1010, 1412, 1811, 2273
Fsp4HI GCNGC 5 cut(s) 1187, 1719, 1722, 1754, 2133
FspBI CTAG 4 cut(s) 431, 930, 1305, 1767
GlaI GCGC 1 cut(s) 415
GluI GCNGC 5 cut(s) 1187, 1719, 1722, 1754, 2133
HaeIII GGCC 4 cut(s) 474, 691, 1362, 1556
HhaI GCGC 1 cut(s) 416
Hin1I GRCGYC 1 cut(s) 908
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HindIII AAGCTT 2 cut(s) 723, 1169
HinfI GANTC 9 cut(s) 236, 257, 487, 857, 1156, 1301, 1853, 2143, 2192
HphI GGTGA 6 cut(s) 60, 217, 638, 695, 866, 1603
Hpy166II GTNNAC 5 cut(s) 514, 764, 1121, 1672, 2057
Hpy188I TCNGA 4 cut(s) 367, 802, 1107, 2197
Hpy188III TCNNGA 9 cut(s) 168, 286, 837, 875, 1759, 1767, 1819, 2102, 2201
Hpy8I GTNNAC 5 cut(s) 514, 764, 1121, 1672, 2057
Hpy99I CGWCG 1 cut(s) 913
HpyCH4III ACNGT 5 cut(s) 89, 448, 1621, 1676, 1776
HpyCH4IV ACGT 2 cut(s) 183, 908
HpyF10VI GCNNNNNNNGC 4 cut(s) 413, 1856, 2174, 2183
HpyF3I CTNAG 7 cut(s) 573, 1104, 1175, 1233, 1551, 1825, 2178
HpySE526I ACGT 2 cut(s) 183, 908
Hsp92I GRCGYC 1 cut(s) 908
HspAI GCGC 1 cut(s) 414
KflI GGGWCCC 1 cut(s) 374
Kzo9I GATC 8 cut(s) 892, 932, 1097, 1350, 1701, 1821, 2120, 2197
LmnI GCTCC 2 cut(s) 368, 740
Lsp1109I GCAGC 4 cut(s) 1198, 1708, 1765, 2119
LweI GCATC 7 cut(s) 535, 651, 1266, 1655, 1789, 2254, 2277
MabI ACCWGGT 1 cut(s) 850
MaeI CTAG 4 cut(s) 431, 930, 1305, 1767
MaeII ACGT 2 cut(s) 183, 908
MaeIII GTNAC 6 cut(s) 323, 1078, 1414, 1501, 1615, 1676
MalI GATC 8 cut(s) 894, 934, 1099, 1352, 1703, 1823, 2122, 2199
MboI GATC 8 cut(s) 892, 932, 1097, 1350, 1701, 1821, 2120, 2197
MboII GAAGA 7 cut(s) 19, 51, 231, 383, 887, 1285, 1916
MflI RGATCY 3 cut(s) 892, 932, 2120
MlsI TGGCCA 1 cut(s) 1362
MluNI TGGCCA 1 cut(s) 1362
MlyI GAGTC 1 cut(s) 1150
MmeI TCCRAC 4 cut(s) 99, 135, 572, 2256
Mox20I TGGCCA 1 cut(s) 1362
Mph1103I ATGCAT 3 cut(s) 550, 666, 2320
MscI TGGCCA 1 cut(s) 1362
MseI TTAA 5 cut(s) 84, 1386, 1653, 1683, 1896
MslI CAYNNNNRTG 3 cut(s) 1433, 1517, 1845
Msp20I TGGCCA 1 cut(s) 1362
MspA1I CMGCKG 1 cut(s) 1721
MspR9I CCNGG 1 cut(s) 852
Mva1269I GAATGC 3 cut(s) 956, 1064, 1989
MvaI CCWGG 1 cut(s) 852
MvnI CGCG 1 cut(s) 482
MwoI GCNNNNNNNGC 4 cut(s) 413, 1856, 2174, 2183
NcoI CCATGG 1 cut(s) 436
NdeII GATC 8 cut(s) 892, 932, 1097, 1350, 1701, 1821, 2120, 2197
NlaIV GGNNCC 3 cut(s) 364, 375, 376
NmeAIII GCCGAG 3 cut(s) 442, 450, 1583
NmuCI GTSAC 2 cut(s) 1078, 1676
NsiI ATGCAT 3 cut(s) 550, 666, 2320
NspI RCATGY 5 cut(s) 520, 552, 1021, 1850, 2186
PaeI GCATGC 2 cut(s) 1021, 2186
PaeR7I CTCGAG 1 cut(s) 338
PagI TCATGA 1 cut(s) 1818
PciI ACATGT 1 cut(s) 516
PctI GAATGC 3 cut(s) 956, 1064, 1989
PfeI GAWTC 8 cut(s) 236, 257, 487, 857, 1301, 1853, 2143, 2192
PflFI GACNNNGTC 1 cut(s) 1406
PflMI CCANNNNNTGG 1 cut(s) 1879
PkrI GCNGC 5 cut(s) 1188, 1720, 1723, 1755, 2134
PleI GAGTC 1 cut(s) 1150
PpsI GAGTC 1 cut(s) 1150
PpuMI RGGWCCY 1 cut(s) 374
PscI ACATGT 1 cut(s) 516
PsiI TTATAA 1 cut(s) 605
Psp1406I AACGTT 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 850
PspEI GGTNACC 1 cut(s) 1414
PspGI CCWGG 1 cut(s) 850
PspN4I GGNNCC 3 cut(s) 364, 375, 376
PspPI GGNCC 1 cut(s) 374
PspPPI RGGWCCY 1 cut(s) 374
PspXI VCTCGAGB 1 cut(s) 338
PstNI CAGNNNCTG 2 cut(s) 806, 1856
PsuI RGATCY 3 cut(s) 892, 932, 2120
PsyI GACNNNGTC 1 cut(s) 1406
RsaI GTAC 2 cut(s) 521, 1136
RsaNI GTAC 2 cut(s) 520, 1135
RseI CAYNNNNRTG 3 cut(s) 1433, 1517, 1845
SaqAI TTAA 5 cut(s) 84, 1386, 1653, 1683, 1896
SatI GCNGC 5 cut(s) 1187, 1719, 1722, 1754, 2133
Sau3AI GATC 8 cut(s) 892, 932, 1097, 1350, 1701, 1821, 2120, 2197
Sau96I GGNCC 1 cut(s) 374
SchI GAGTC 1 cut(s) 1150
ScrFI CCNGG 1 cut(s) 852
SexAI ACCWGGT 1 cut(s) 850
SfaNI GCATC 7 cut(s) 535, 651, 1266, 1655, 1789, 2254, 2277
SfcI CTRYAG 2 cut(s) 744, 2187
Sfr274I CTCGAG 1 cut(s) 338
SinI GGWCC 1 cut(s) 374
SlaI CTCGAG 1 cut(s) 338
SmiMI CAYNNNNRTG 3 cut(s) 1433, 1517, 1845
SmlI CTYRAG 2 cut(s) 338, 1757
SmoI CTYRAG 2 cut(s) 338, 1757
SphI GCATGC 2 cut(s) 1021, 2186
SsiI CCGC 6 cut(s) 482, 492, 531, 599, 995, 1719
SspI AATATT 1 cut(s) 1283
SspMI CTAG 4 cut(s) 431, 930, 1305, 1767
StyD4I CCNGG 1 cut(s) 850
StyI CCWWGG 2 cut(s) 436, 1069
TaaI ACNGT 5 cut(s) 89, 448, 1621, 1676, 1776
TaiI ACGT 2 cut(s) 186, 911
TaqI TCGA 5 cut(s) 339, 524, 535, 561, 1394
TatI WGTACW 2 cut(s) 519, 1134
TauI GCSGC 1 cut(s) 1721
TfiI GAWTC 8 cut(s) 236, 257, 487, 857, 1301, 1853, 2143, 2192
Tru1I TTAA 5 cut(s) 84, 1386, 1653, 1683, 1896
Tru9I TTAA 5 cut(s) 84, 1386, 1653, 1683, 1896
TscAI CASTG 2 cut(s) 711, 1679
TseFI GTSAC 2 cut(s) 1078, 1676
TseI GCWGC 4 cut(s) 1186, 1721, 1753, 2132
Tsp45I GTSAC 2 cut(s) 1078, 1676
TspGWI ACGGA 2 cut(s) 1702, 1857
TspRI CASTG 2 cut(s) 711, 1679
Tth111I GACNNNGTC 1 cut(s) 1406
Van91I CCANNNNNTGG 1 cut(s) 1879
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 7 cut(s) 694, 1036, 1263, 1473, 1543, 1866, 1991
XbaI TCTAGA 1 cut(s) 1766
XceI RCATGY 5 cut(s) 520, 552, 1021, 1850, 2186
XcmI CCANNNNNNNNNTGG 2 cut(s) 314, 1370
XhoI CTCGAG 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 513
XspI CTAG 4 cut(s) 431, 930, 1305, 1767
ZraI GACGTC 1 cut(s) 909
Zsp2I ATGCAT 3 cut(s) 550, 666, 2320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.