Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000152

Physical Location & Seq

Genomic Coordinates Forward (+)

20289455 .. 20290990

Gene Structure 1536 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1245600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 981 bp

ATGGACCCTCAACAAAACCCAAATGAACTATTAGACGGCGGCAACAGGCAGGGGGGTGGAGTCATGTGCAGGCAAAGCAGTACGCGGTGGACACCCACCACTGATCAGATAAAAATCTTGAAGGACCTATACTACAACAATGGCATTAGATCACCCAGCGCTGAGCAGATTCACAGGATCTCTGCTAAACTGCGACAGTACGGCAAGATCGAAGGCAAGAACGTTTTCTATTGGTTTCAGAACCACAAAGCTCGTGAGAGGCAGAAGAAAAGATTCACTTCTTCTTCTACTCCTGATCATCACGCAGCACCACCAATGCCAGCTGTACCACCGCAAGCCGATAATATCAGGCAAAGATCATCAGGGTTTGGGATTGATATTAATGCAACTGCTGCTGCTGCTTATGAACAACTACCCATTAATCACCACAGCAAGTATTCCAACATTTCTGGTTCACCAGCTGGGTTTTCTTCTACATCTTCTTCAGTTGGTGTGAATGTTTCTGCTGGGGCACAGATGGGAAACTATGGGTATGGATCCATGGCCATGGAGAAGAGCTTTAGGGACTGCTCACTATCATCTGGAGGAAGTACTAGCACCGGTCATGTTGGTGGATCTAATTATAATAATTTTAATCACAACCCAGGATCATGGGTTGGTGTTGATCCATATTCCTCACCCTACTCCATCTTTGACAAGAAATCACCATCAAAACAAGTGTTTGGTGATCAAGAAAACATGACGGAAGAAGAATATTACAATCAGCAGCAAGCTTCACCAGAGATTGAGACTCTCCCTCTTTTCCCCATGCATGGTGAAGACATCCATGGTTTTGGCAACATCAAGTCCTCCTCCATGGAAGGCTACTACTCCGGCTGGTACCGCTCCGACGGCAGCAACGATGGTGGGTCTCGCACTTCCCTTGAGCTTAGCCTCAATTCCTACGGCCACATGGCCCCTGATTGCTTCAGATCATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009908 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010223 GO:0010346 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019827 GO:0022412 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032504 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048532 GO:0048608 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0080166 GO:0090506 GO:0090567 GO:0098727 GO:0099402 GO:0140110 GO:1903506 GO:1905393 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox

Protein Analysis

327

Amino Acids

35.79

Weight (kDa)

6.83

Isoelectric Point (pI)

60.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	28 - 88	9.3e-13	Homeodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000152)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17950
fragaria_vesca	FvH4_1g11910 FvH4_3g04400
malus_domestica	MD02G1133000.v1.1 MD15G1245600.v1.1
prunus_persica	Prupe.7G167700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0314921 RchiOBHm_Chr1g0319941 RchiOBHm_Chr1g0321251 RchiOBHm_Chr1g0323021 RchiOBHm_Chr1g0324491 RchiOBHm_Chr1g0325171 RchiOBHm_Chr1g0360601 RchiOBHm_Chr1g0365451 RchiOBHm_Chr1g0381201 RchiOBHm_Chr2g0085401 RchiOBHm_Chr2g0099501 RchiOBHm_Chr2g0106981 RchiOBHm_Chr2g0130271 RchiOBHm_Chr2g0155121 RchiOBHm_Chr3g0455971 RchiOBHm_Chr3g0459501 RchiOBHm_Chr3g0478111 RchiOBHm_Chr3g0480661 RchiOBHm_Chr4g0388561 RchiOBHm_Chr4g0394421 RchiOBHm_Chr4g0424371 RchiOBHm_Chr5g0028631 RchiOBHm_Chr5g0069751 RchiOBHm_Chr5g0076691 RchiOBHm_Chr5g0076711 RchiOBHm_Chr5g0076721 RchiOBHm_Chr5g0079671 RchiOBHm_Chr5g0079731 RchiOBHm_Chr5g0079911 RchiOBHm_Chr5g0081741 RchiOBHm_Chr6g0255231 RchiOBHm_Chr6g0283251 RchiOBHm_Chr6g0300091 RchiOBHm_Chr7g0190751 RchiOBHm_Chr7g0240241
rosa_multiflora	Rmu_co8441375.1_g000001 Rmu_sc0000110.1_g000027 Rmu_sc0000187.1_g000008 Rmu_sc0000240.1_g000065 Rmu_sc0000251.1_g000004 Rmu_sc0000272.1_g000025 Rmu_sc0000276.1_g000074 Rmu_sc0000284.1_g000010 Rmu_sc0000285.1_g000021 Rmu_sc0000360.1_g000034 Rmu_sc0000365.1_g000038 Rmu_sc0000368.1_g000013 Rmu_sc0000368.1_g000025 Rmu_sc0000770.1_g000044 Rmu_sc0000770.1_g000052 Rmu_sc0000795.1_g000098 Rmu_sc0000945.1_g000037 Rmu_sc0000949.1_g000023 Rmu_sc0000982.1_g000002 Rmu_sc0001424.1_g000004 Rmu_sc0001775.1_g000004 Rmu_sc0001952.1_g000010 Rmu_sc0002080.1_g000046 Rmu_sc0002141.1_g000016 Rmu_sc0002183.1_g000004 Rmu_sc0002187.1_g000040 Rmu_sc0002263.1_g000057 Rmu_sc0002351.1_g000061 Rmu_sc0002542.1_g000017 Rmu_sc0002751.1_g000003 Rmu_sc0002792.1_g000029 Rmu_sc0002798.1_g000020 Rmu_sc0002985.1_g000010 Rmu_sc0003124.1_g000019 Rmu_sc0003207.1_g000019 Rmu_sc0003237.1_g000003 Rmu_sc0003363.1_g000050 Rmu_sc0003443.1_g000025 Rmu_sc0003465.1_g000032 Rmu_sc0003500.1_g000012 Rmu_sc0003809.1_g000026 Rmu_sc0003859.1_g000032 Rmu_sc0004085.1_g000003 Rmu_sc0004085.1_g000008 Rmu_sc0004571.1_g000019 Rmu_sc0004753.1_g000006 Rmu_sc0004865.1_g000017 Rmu_sc0004881.1_g000007 Rmu_sc0005697.1_g000023 Rmu_sc0005812.1_g000020 Rmu_sc0007076.1_g000001 Rmu_sc0007801.1_g000003 Rmu_sc0008355.1_g000007 Rmu_sc0009380.1_g000006 Rmu_sc0010191.1_g000004 Rmu_sc0010252.1_g000006 Rmu_sc0011026.1_g000001 Rmu_sc0011824.1_g000003 Rmu_sc0012533.1_g000006 Rmu_sc0012640.1_g000006 Rmu_sc0014032.1_g000001 Rmu_sc0014545.1_g000001 Rmu_sc0014840.1_g000008 Rmu_sc0015060.1_g000013 Rmu_sc0018122.1_g000003 Rmu_sc0026563.1_g000004 Rmu_ssc0000213.1_g000057 Rmu_ssc0000213.1_g000091 Rmu_ssc0000262.1_g000021 Rmu_ssc0000282.1_g000021 Rmu_ssc0000295.1_g000041 Rmu_ssc0000438.1_g000002 Rmu_ssc0000472.1_g000015
rosa_roxburghii	Rroxscaffold_1G00020080 Rroxscaffold_2G00084980 Rroxscaffold_2G00143020 Rroxscaffold_3G00261750 Rroxscaffold_3G00268610 Rroxscaffold_4G00312950 Rroxscaffold_7G00156650 Rroxscaffold_7G00215160
rosa_rugosa	Rorug01G0077100 Rorug01G0201100 Rorug01G0246900 Rorug01G0256600 Rorug02G0080200 Rorug02G0089300 Rorug02G0090700 Rorug02G0177200 Rorug02G0187000 Rorug02G0192600 Rorug02G0232700 Rorug02G0262100 Rorug02G0328300 Rorug02G0408300 Rorug02G0641200 Rorug03G0155000 Rorug03G0185500 Rorug03G0256500 Rorug03G0291000 Rorug03G0299400 Rorug03G0317000 Rorug03G0326700 Rorug03G0330800 Rorug03G0339700 Rorug03G0352900 Rorug04G0029800 Rorug04G0029900 Rorug04G0048100 Rorug04G0075800 Rorug04G0124400 Rorug04G0212100 Rorug04G0212400 Rorug04G0253200 Rorug05G0116700 Rorug05G0146500 Rorug05G0211900 Rorug05G0215700 Rorug05G0216100 Rorug05G0220500 Rorug05G0339400 Rorug05G0352000 Rorug05G0356500.1 Rorug05G0398900 Rorug05G0474700 Rorug05G0511400 Rorug05G0540200 Rorug05G0541000 Rorug05G0572900.1 Rorug05G0584000 Rorug06G0000900 Rorug06G0014600 Rorug06G0018800 Rorug06G0026100 Rorug06G0042900 Rorug06G0082600 Rorug06G0133900 Rorug06G0229100 Rorug07G0321400 Rorug07G0326400 Rorug07G0326600.1
rosa_wichuraiana	Rw0G017710 Rw1G012170 Rw1G039350 Rw2G010030 Rw3G024430 Rw5G045720 Rw7G025340 Rw7G040870

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	624
Acc65I	GGTACC	1 cut(s)	879
AccB1I	GGYRCC	1 cut(s)	879
AccBSI	CCGCTC	1 cut(s)	885
AccII	CGCG	1 cut(s)	85
AciI	CCGC	4 cut(s)	39, 85, 332, 883
AclI	AACGTT	1 cut(s)	222
AclWI	GGATC	6 cut(s)	185, 531, 544, 622, 655, 659
AcoI	YGGCCR	2 cut(s)	543, 946
AcuI	CTGAAG	2 cut(s)	468, 952
AfaI	GTAC	5 cut(s)	82, 200, 327, 592, 881
AfeI	AGCGCT	1 cut(s)	160
AfiI	CCNNNNNNNGG	1 cut(s)	812
AgeI	ACCGGT	1 cut(s)	599
AgsI	TTSAA	1 cut(s)	121
AjnI	CCWGG	1 cut(s)	643
AluBI	AGCT	6 cut(s)	251, 323, 461, 558, 773, 928
AluI	AGCT	6 cut(s)	251, 323, 461, 558, 773, 928
Alw26I	GTCTC	2 cut(s)	782, 915
AlwI	GGATC	6 cut(s)	185, 531, 544, 622, 655, 659
Aor51HI	AGCGCT	1 cut(s)	160
AoxI	GGCC	3 cut(s)	543, 946, 954
ApeKI	GCWGC	6 cut(s)	305, 392, 395, 398, 766, 894
AseI	ATTAAT	2 cut(s)	381, 420
AsiGI	ACCGGT	1 cut(s)	599
Asp700I	GAANNNNTTC	2 cut(s)	224, 272
Asp718I	GGTACC	1 cut(s)	879
AspLEI	GCGC	1 cut(s)	161
AspS9I	GGNCC	3 cut(s)	4, 124, 955
AsuHPI	GGTGA	8 cut(s)	144, 416, 447, 669, 696, 737, 768, 827
AvaII	GGWCC	2 cut(s)	4, 124
BaeGI	GKGCMC	1 cut(s)	514
BalI	TGGCCA	1 cut(s)	545
BamHI	GGATCC	1 cut(s)	536
BanI	GGYRCC	1 cut(s)	879
BarI	GAAGNNNNNNTAC	2 cut(s)	113, 145
BauI	CACGAG	1 cut(s)	252
BbsI	GAAGAC	1 cut(s)	825
BbvI	GCAGC	6 cut(s)	317, 379, 382, 385, 778, 906
BccI	CCATC	4 cut(s)	511, 695, 715, 896
BceAI	ACGGC	4 cut(s)	52, 217, 907, 961
BciT130I	CCWGG	1 cut(s)	645
BclI	TGATCA	3 cut(s)	103, 295, 727
BcoDI	GTCTC	2 cut(s)	782, 915
BfaI	CTAG	1 cut(s)	594
BfoI	RGCGCY	1 cut(s)	162
BisI	GCNGC	7 cut(s)	40, 306, 393, 396, 399, 767, 895
BlpI	GCTNAGC	2 cut(s)	162, 929
BlsI	GCNGC	7 cut(s)	41, 307, 394, 397, 400, 768, 896
BmcAI	AGTACT	1 cut(s)	592
Bme1390I	CCNGG	1 cut(s)	645
Bme18I	GGWCC	2 cut(s)	4, 124
BmgT120I	GGNCC	3 cut(s)	4, 124, 955
BmiI	GGNNCC	4 cut(s)	6, 538, 881, 957
BmrFI	CCNGG	1 cut(s)	645
BpiI	GAAGAC	1 cut(s)	825
BpmI	CTGGAG	1 cut(s)	603
Bpu1102I	GCTNAGC	2 cut(s)	162, 929
BpuEI	CTTGAG	1 cut(s)	944
BsaBI	GATNNNNATC	1 cut(s)	113
BsaI	GGTCTC	1 cut(s)	915
BsaJI	CCNNGG	5 cut(s)	540, 546, 643, 826, 855
BsaWI	WCCGGW	1 cut(s)	599
Bsc4I	CCNNNNNNNGG	1 cut(s)	812
Bse118I	RCCGGY	1 cut(s)	599
Bse8I	GATNNNNATC	1 cut(s)	113
BseBI	CCWGG	1 cut(s)	645
BseDI	CCNNGG	5 cut(s)	540, 546, 643, 826, 855
BseGI	GGATG	1 cut(s)	822
BseJI	GATNNNNATC	1 cut(s)	113
BseLI	CCNNNNNNNGG	1 cut(s)	812
BseMII	CTCAG	1 cut(s)	153
BseRI	GAGGAG	1 cut(s)	841
BseSI	GKGCMC	1 cut(s)	514
BseXI	GCAGC	6 cut(s)	317, 379, 382, 385, 778, 906
BseYI	CCCAGC	3 cut(s)	155, 461, 506
BsgI	GTGCAG	1 cut(s)	88
Bsh1236I	CGCG	1 cut(s)	85
BshFI	GGCC	3 cut(s)	545, 948, 956
BshNI	GGYRCC	1 cut(s)	879
BshTI	ACCGGT	1 cut(s)	599
BsiSI	CCGG	2 cut(s)	600, 873
BslFI	GGGAC	1 cut(s)	578
BslI	CCNNNNNNNGG	1 cut(s)	812
BsmAI	GTCTC	2 cut(s)	782, 915
BsmFI	GGGAC	1 cut(s)	578
BsnI	GGCC	3 cut(s)	545, 948, 956
Bso31I	GGTCTC	1 cut(s)	915
Bsp1286I	GDGCHC	1 cut(s)	514
Bsp1720I	GCTNAGC	2 cut(s)	162, 929
Bsp19I	CCATGG	4 cut(s)	540, 546, 826, 855
BspACI	CCGC	4 cut(s)	39, 85, 332, 883
BspANI	GGCC	3 cut(s)	545, 948, 956
BspCNI	CTCAG	1 cut(s)	154
BspFNI	CGCG	1 cut(s)	85
BspLI	GGNNCC	4 cut(s)	6, 538, 881, 957
BspPI	GGATC	6 cut(s)	185, 531, 544, 622, 655, 659
BspQI	GCTCTTC	1 cut(s)	548
BspT107I	GGYRCC	1 cut(s)	879
BspTNI	GGTCTC	1 cut(s)	915
BsrBI	CCGCTC	1 cut(s)	885
BsrFI	RCCGGY	1 cut(s)	599
BssAI	RCCGGY	1 cut(s)	599
BssECI	CCNNGG	5 cut(s)	540, 546, 643, 826, 855
BssSI	CACGAG	1 cut(s)	252
BssT1I	CCWWGG	4 cut(s)	540, 546, 826, 855
Bst2BI	CACGAG	1 cut(s)	252
Bst2UI	CCWGG	1 cut(s)	645
Bst4CI	ACNGT	1 cut(s)	198
Bst6I	CTCTTC	1 cut(s)	548
BstAPI	GCANNNNNTGC	1 cut(s)	392
BstC8I	GCNNGC	4 cut(s)	71, 321, 336, 771
BstDEI	CTNAG	2 cut(s)	162, 929
BstDSI	CCRYGG	4 cut(s)	540, 546, 826, 855
BstF5I	GGATG	1 cut(s)	822
BstFNI	CGCG	1 cut(s)	85
BstH2I	RGCGCY	1 cut(s)	162
BstHHI	GCGC	1 cut(s)	161
BstMAI	GTCTC	2 cut(s)	782, 915
BstMWI	GCNNNNNNNGC	5 cut(s)	75, 392, 398, 882, 891
BstNI	CCWGG	1 cut(s)	645
BstSCI	CCNGG	1 cut(s)	643
BstSLI	GKGCMC	1 cut(s)	514
BstUI	CGCG	1 cut(s)	85
BstV1I	GCAGC	6 cut(s)	317, 379, 382, 385, 778, 906
BstV2I	GAAGAC	1 cut(s)	825
BstX2I	RGATCY	3 cut(s)	177, 536, 614
BstXI	CCANNNNNNTGG	3 cut(s)	547, 651, 833
BstYI	RGATCY	3 cut(s)	177, 536, 614
BsuRI	GGCC	3 cut(s)	545, 948, 956
BtgI	CCRYGG	4 cut(s)	540, 546, 826, 855
BtsCI	GGATG	1 cut(s)	822
BtsIMutI	CAGTG	1 cut(s)	99
Cac8I	GCNNGC	4 cut(s)	71, 321, 336, 771
CfoI	GCGC	1 cut(s)	161
Cfr10I	RCCGGY	1 cut(s)	599
Cfr13I	GGNCC	3 cut(s)	4, 124, 955
Csp6I	GTAC	5 cut(s)	81, 199, 326, 591, 880
CspAI	ACCGGT	1 cut(s)	599
CspCI	CAANNNNNGTGG	2 cut(s)	886, 921
CviQI	GTAC	5 cut(s)	81, 199, 326, 591, 880
DdeI	CTNAG	2 cut(s)	162, 929
EaeI	YGGCCR	2 cut(s)	543, 946
Eam1104I	CTCTTC	1 cut(s)	548
EarI	CTCTTC	1 cut(s)	548
Eco130I	CCWWGG	4 cut(s)	540, 546, 826, 855
Eco31I	GGTCTC	1 cut(s)	915
Eco47I	GGWCC	2 cut(s)	4, 124
Eco47III	AGCGCT	1 cut(s)	160
Eco57I	CTGAAG	2 cut(s)	468, 952
EcoO109I	RGGNCCY	1 cut(s)	124
EcoRII	CCWGG	1 cut(s)	643
EcoT14I	CCWWGG	4 cut(s)	540, 546, 826, 855
EcoT22I	ATGCAT	1 cut(s)	813
ErhI	CCWWGG	4 cut(s)	540, 546, 826, 855
FalI	AAGNNNNNCTT	2 cut(s)	262, 294
FaqI	GGGAC	1 cut(s)	578
FbaI	TGATCA	3 cut(s)	103, 295, 727
Fnu4HI	GCNGC	7 cut(s)	40, 306, 393, 396, 399, 767, 895
FokI	GGATG	1 cut(s)	809
Fsp4HI	GCNGC	7 cut(s)	40, 306, 393, 396, 399, 767, 895
FspBI	CTAG	1 cut(s)	594
GlaI	GCGC	1 cut(s)	160
GluI	GCNGC	7 cut(s)	40, 306, 393, 396, 399, 767, 895
GsaI	CCCAGC	3 cut(s)	159, 465, 510
GsuI	CTGGAG	1 cut(s)	603
HaeII	RGCGCY	1 cut(s)	162
HaeIII	GGCC	3 cut(s)	545, 948, 956
HapII	CCGG	2 cut(s)	600, 873
HhaI	GCGC	1 cut(s)	161
Hin6I	GCGC	1 cut(s)	159
HinP1I	GCGC	1 cut(s)	159
HindIII	AAGCTT	1 cut(s)	771
HinfI	GANTC	4 cut(s)	60, 169, 273, 790
HpaII	CCGG	2 cut(s)	600, 873
HphI	GGTGA	8 cut(s)	144, 416, 447, 669, 696, 737, 768, 827
Hpy166II	GTNNAC	2 cut(s)	90, 455
Hpy188I	TCNGA	4 cut(s)	108, 240, 889, 971
Hpy188III	TCNNGA	5 cut(s)	118, 254, 293, 582, 731
Hpy8I	GTNNAC	2 cut(s)	90, 455
Hpy99I	CGWCG	1 cut(s)	893
HpyAV	CCTTC	3 cut(s)	115, 206, 854
HpyCH4III	ACNGT	1 cut(s)	198
HpyCH4IV	ACGT	1 cut(s)	222
HpyCH4V	TGCA	3 cut(s)	69, 386, 811
HpyF10VI	GCNNNNNNNGC	5 cut(s)	75, 392, 398, 882, 891
HpyF3I	CTNAG	2 cut(s)	162, 929
HpySE526I	ACGT	1 cut(s)	222
HspAI	GCGC	1 cut(s)	159
KpnI	GGTACC	1 cut(s)	883
Ksp22I	TGATCA	3 cut(s)	103, 295, 727
LguI	GCTCTTC	1 cut(s)	548
LmnI	GCTCC	1 cut(s)	890
Lsp1109I	GCAGC	6 cut(s)	317, 379, 382, 385, 778, 906
MaeI	CTAG	1 cut(s)	594
MaeII	ACGT	1 cut(s)	222
MbiI	CCGCTC	1 cut(s)	885
MflI	RGATCY	3 cut(s)	177, 536, 614
MhlI	GDGCHC	1 cut(s)	514
MlsI	TGGCCA	1 cut(s)	545
MluCI	AATT	3 cut(s)	619, 628, 937
MluNI	TGGCCA	1 cut(s)	545
MlyI	GAGTC	2 cut(s)	69, 784
MmeI	TCCRAC	2 cut(s)	465, 912
MnlI	CCTC	8 cut(s)	18, 252, 578, 685, 807, 859, 862, 944
Mox20I	TGGCCA	1 cut(s)	545
Mph1103I	ATGCAT	1 cut(s)	813
MroXI	GAANNNNTTC	2 cut(s)	224, 272
MscI	TGGCCA	1 cut(s)	545
MseI	TTAA	4 cut(s)	381, 420, 633, 979
MslI	CAYNNNNRTG	2 cut(s)	545, 609
Msp20I	TGGCCA	1 cut(s)	545
MspA1I	CMGCKG	2 cut(s)	323, 461
MspI	CCGG	2 cut(s)	600, 873
MspR9I	CCNGG	1 cut(s)	645
MvaI	CCWGG	1 cut(s)	645
MvnI	CGCG	1 cut(s)	85
MwoI	GCNNNNNNNGC	5 cut(s)	75, 392, 398, 882, 891
NcoI	CCATGG	4 cut(s)	540, 546, 826, 855
NlaIV	GGNNCC	4 cut(s)	6, 538, 881, 957
NsiI	ATGCAT	1 cut(s)	813
PciSI	GCTCTTC	1 cut(s)	548
PcsI	WCGNNNNNNNCGW	2 cut(s)	207, 897
PdmI	GAANNNNTTC	2 cut(s)	224, 272
PfeI	GAWTC	2 cut(s)	169, 273
PinAI	ACCGGT	1 cut(s)	599
PkrI	GCNGC	7 cut(s)	41, 307, 394, 397, 400, 768, 896
PleI	GAGTC	2 cut(s)	68, 784
PpsI	GAGTC	2 cut(s)	68, 784
PpuMI	RGGWCCY	1 cut(s)	124
PshBI	ATTAAT	2 cut(s)	381, 420
PsiI	TTATAA	1 cut(s)	624
Psp1406I	AACGTT	1 cut(s)	222
Psp5II	RGGWCCY	1 cut(s)	124
Psp6I	CCWGG	1 cut(s)	643
PspFI	CCCAGC	3 cut(s)	155, 461, 506
PspGI	CCWGG	1 cut(s)	643
PspN4I	GGNNCC	4 cut(s)	6, 538, 881, 957
PspPI	GGNCC	3 cut(s)	4, 124, 955
PspPPI	RGGWCCY	1 cut(s)	124
PsuI	RGATCY	3 cut(s)	177, 536, 614
PvuII	CAGCTG	2 cut(s)	323, 461
RsaI	GTAC	5 cut(s)	82, 200, 327, 592, 881
RsaNI	GTAC	5 cut(s)	81, 199, 326, 591, 880
RseI	CAYNNNNRTG	2 cut(s)	545, 609
SapI	GCTCTTC	1 cut(s)	548
SaqAI	TTAA	4 cut(s)	381, 420, 633, 979
SatI	GCNGC	7 cut(s)	40, 306, 393, 396, 399, 767, 895
Sau96I	GGNCC	3 cut(s)	4, 124, 955
ScaI	AGTACT	1 cut(s)	592
SchI	GAGTC	2 cut(s)	69, 784
ScrFI	CCNGG	1 cut(s)	645
SduI	GDGCHC	1 cut(s)	514
SetI	ASST	8 cut(s)	129, 225, 253, 325, 463, 560, 775, 930
SinI	GGWCC	2 cut(s)	4, 124
SmiMI	CAYNNNNRTG	2 cut(s)	545, 609
SmlI	CTYRAG	1 cut(s)	923
SmoI	CTYRAG	1 cut(s)	923
Sse9I	AATT	3 cut(s)	619, 628, 937
SsiI	CCGC	4 cut(s)	39, 85, 332, 883
SspI	AATATT	1 cut(s)	755
SspMI	CTAG	1 cut(s)	594
StyD4I	CCNGG	1 cut(s)	643
StyI	CCWWGG	4 cut(s)	540, 546, 826, 855
TaaI	ACNGT	1 cut(s)	198
TaiI	ACGT	1 cut(s)	225
TaqI	TCGA	1 cut(s)	210
TasI	AATT	3 cut(s)	619, 628, 937
TatI	WGTACW	1 cut(s)	590
TauI	GCSGC	1 cut(s)	42
TfiI	GAWTC	2 cut(s)	169, 273
Tru1I	TTAA	4 cut(s)	381, 420, 633, 979
Tru9I	TTAA	4 cut(s)	381, 420, 633, 979
TscAI	CASTG	1 cut(s)	106
TseI	GCWGC	6 cut(s)	305, 392, 395, 398, 766, 894
TspDTI	ATGAA	2 cut(s)	39, 420
TspGWI	ACGGA	1 cut(s)	758
TspRI	CASTG	1 cut(s)	106
VpaK11BI	GGWCC	2 cut(s)	4, 124
VspI	ATTAAT	2 cut(s)	381, 420
XmnI	GAANNNNTTC	2 cut(s)	224, 272
XspI	CTAG	1 cut(s)	594
ZrmI	AGTACT	1 cut(s)	592
Zsp2I	ATGCAT	1 cut(s)	813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.