Rroxscaffold_2G00130510

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013978
Physical Location & Seq
Reverse (-)
66714138 .. 66715849
1712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00130510.1

Sequence Viewer

Length: 1065 bp
ATGGCAGCAGCTATAGATATTTACAGTAGCAGCAGCTCAATGTCACCAGATTTATCAGAGGAGCTTATGAGAGCGCTTATGCCTTATATGAAAAGTGCTTCTTCAACCTCTTCTATTTCTCCACCAACATCTCCTTCCACTTCTTCTTCTTCTTCTTCTTCCTACACTCCATTTTCCTCTTCTTCTTATCCCATTTTGTACCCTGACTTTTGCTCAAACAGCCAAATGTTCCCACAAGTGGGTTTTGAGCAAACAGGTTCATTGGGGCTTAACCAACTCACCCCATCTCAAATTCGCCAAATCCAAACCCAAATGATACTCCAACAACAGCACCAACAACAACAGAAGCAGCAAATTGCAGCTCTAGCTCCACTACCAAGCCAATATCACCACCAACACTCTCACACCCTCAACTTCCTTGGCCCAAAAGCAATCCCAATGAAGCAATCTGGTACTCCCAAGCCCACAAAGCTCTACAGGGGAGTGAGGCAGAGGCATTGGGGAAAATGGGTTGCTGAGATTAGACTCCCCAAGAACCGAACTCGCCTCTGGCTTGGCACATTCGAAACAGCCGAAGAAGCAGCTTTAGCTTATGATAAAGCTGCTTATAAGCTCAGGGGAGACTTTGCCAGGCTCAATTTCCCTCATCTTAAGCATCAAGGAGCTCATATCACTGGAGATTTTGGCAATTACAAGCCTCTTCCTTCCGCAGTTGACGCCAAGCTCCAAGCAATTTGCGAGAGCTTGGCTGTTAATTCGCCGAAACAGGGCAAAACAGAGGAGCTCTGTTCTGAAACAGAGACAAAGCCAGTGATTTCAGCTCCAAAGATGGTCTTTGATGATTCATTGAGTACAGATTTGAAAAGAGAATGGGACTCTCCTAAATTGGAGGCCATTTCATGGTCATCTTCACCCTCTCGATCCTGCGATGAGTCCTCAGCCGGGTCATCTTCACCGGAATCTGACATTACATTCTTGGATTTCTCAGATTCTCAGTGGGATGAGAATGATACTTTCGGATTGGAGAAGTACCCTTCAGTGGAGATTGATTGGTCTGCTATTTAA

Protein Analysis

354

Amino Acids

39.25

Weight (kDa)

6.0

Isoelectric Point (pI)

66.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 158 - 207 3.1e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22200 AT4G39780
fragaria_vesca FvH4_1g21210
malus_domestica MD15G1326800.v1.1
prunus_persica Prupe.6G182200_v2.0.a1
pyrus_communis pycom01g05500 pycom395g00070
rosa_chinensis RchiOBHm_Chr2g0115041
rosa_laevigata RLG00000018150
rosa_multiflora Rmu_sc0000784.1_g000017
rosa_roxburghii Rroxscaffold_2G00130510
rosa_rugosa Rorug02G0192600
rosa_samantha Rh2AG251200 Rh2BG260700 Rh2CG255800 Rh2DG259200
rosa_wichuraiana Rw2G019610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
AccB7I CCANNNNNTGG 1 cut(s) 900
AciI CCGC 1 cut(s) 708
AclWI GGATC 1 cut(s) 915
AcsI RAATTY 1 cut(s) 291
AcuI CTGAAG 1 cut(s) 1020
AcyI GRCGYC 1 cut(s) 717
AfaI GTAC 4 cut(s) 200, 454, 853, 1031
AfeI AGCGCT 1 cut(s) 75
AfiI CCNNNNNNNGG 6 cut(s) 238, 239, 767, 900, 942, 1039
AflII CTTAAG 1 cut(s) 650
AgsI TTSAA 2 cut(s) 105, 862
AjnI CCWGG 1 cut(s) 629
AjuI GAANNNNNNNTTGG 2 cut(s) 118, 150
Alw21I GWGCWC 2 cut(s) 667, 786
Alw26I GTCTC 2 cut(s) 615, 794
AlwI GGATC 1 cut(s) 915
Aor51HI AGCGCT 1 cut(s) 75
AoxI GGCC 2 cut(s) 421, 891
ApeKI GCWGC 8 cut(s) 5, 8, 30, 33, 349, 359, 581, 602
ApoI RAATTY 1 cut(s) 291
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 422
AsuC2I CCSGG 1 cut(s) 943
AsuHPI GGTGA 5 cut(s) 36, 271, 380, 903, 945
AsuII TTCGAA 1 cut(s) 564
BanII GRGCYC 2 cut(s) 667, 786
Bbv12I GWGCWC 2 cut(s) 667, 786
BbvCI CCTCAGC 1 cut(s) 937
BbvI GCAGC 8 cut(s) 17, 20, 42, 45, 361, 371, 589, 593
BccI CCATC 2 cut(s) 292, 823
BciT130I CCWGG 1 cut(s) 631
BcnI CCSGG 1 cut(s) 943
BcoDI GTCTC 2 cut(s) 615, 794
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 2 cut(s) 12, 475
BfoI RGCGCY 1 cut(s) 77
BfrI CTTAAG 1 cut(s) 650
BisI GCNGC 8 cut(s) 6, 9, 31, 34, 350, 360, 582, 603
BlsI GCNGC 8 cut(s) 7, 10, 32, 35, 351, 361, 583, 604
Bme1390I CCNGG 2 cut(s) 631, 943
BmgT120I GGNCC 1 cut(s) 422
BmrFI CCNGG 2 cut(s) 631, 943
BmsI GCATC 1 cut(s) 664
BpmI CTGGAG 1 cut(s) 696
Bpu10I CCTNAGC 2 cut(s) 614, 937
Bpu14I TTCGAA 1 cut(s) 564
BpuMI CCSGG 1 cut(s) 943
BsaHI GRCGYC 1 cut(s) 717
BsaJI CCNNGG 1 cut(s) 418
BsaWI WCCGGW 1 cut(s) 955
Bsc4I CCNNNNNNNGG 6 cut(s) 238, 239, 767, 900, 942, 1039
Bse1I ACTGG 2 cut(s) 679, 809
BseBI CCWGG 1 cut(s) 631
BseDI CCNNGG 1 cut(s) 418
BseGI GGATG 1 cut(s) 1006
BseLI CCNNNNNNNGG 6 cut(s) 238, 239, 767, 900, 942, 1039
BseMII CTCAG 5 cut(s) 507, 628, 951, 999, 1007
BseNI ACTGG 2 cut(s) 679, 809
BseRI GAGGAG 2 cut(s) 74, 794
BseXI GCAGC 8 cut(s) 17, 20, 42, 45, 361, 371, 589, 593
BshFI GGCC 2 cut(s) 423, 893
BsiHKAI GWGCWC 2 cut(s) 667, 786
BsiSI CCGG 2 cut(s) 942, 956
BslFI GGGAC 1 cut(s) 887
BslI CCNNNNNNNGG 6 cut(s) 238, 239, 767, 900, 942, 1039
BsmAI GTCTC 2 cut(s) 615, 794
BsmFI GGGAC 1 cut(s) 887
BsnI GGCC 2 cut(s) 423, 893
Bsp119I TTCGAA 1 cut(s) 564
Bsp1286I GDGCHC 2 cut(s) 667, 786
Bsp143I GATC 1 cut(s) 920
BspACI CCGC 1 cut(s) 708
BspANI GGCC 2 cut(s) 423, 893
BspCNI CTCAG 5 cut(s) 508, 627, 950, 998, 1006
BspPI GGATC 1 cut(s) 915
BspT104I TTCGAA 1 cut(s) 564
BspTI CTTAAG 1 cut(s) 650
BsrI ACTGG 2 cut(s) 679, 809
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 1 cut(s) 920
BssNI GRCGYC 1 cut(s) 717
BssT1I CCWWGG 1 cut(s) 418
Bst2UI CCWGG 1 cut(s) 631
Bst4CI ACNGT 1 cut(s) 26
Bst6I CTCTTC 3 cut(s) 115, 184, 705
BstACI GRCGYC 1 cut(s) 717
BstAFI CTTAAG 1 cut(s) 650
BstBI TTCGAA 1 cut(s) 564
BstDEI CTNAG 5 cut(s) 516, 614, 937, 985, 993
BstF5I GGATG 1 cut(s) 1006
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 1 cut(s) 923
BstMAI GTCTC 2 cut(s) 615, 794
BstMBI GATC 1 cut(s) 920
BstMWI GCNNNNNNNGC 6 cut(s) 219, 365, 469, 578, 587, 716
BstNI CCWGG 1 cut(s) 631
BstSCI CCNGG 2 cut(s) 629, 941
BstSFI CTRYAG 2 cut(s) 12, 475
BstV1I GCAGC 8 cut(s) 17, 20, 42, 45, 361, 371, 589, 593
BsuRI GGCC 2 cut(s) 423, 893
BtgZI GCGATG 1 cut(s) 942
BtsCI GGATG 1 cut(s) 1006
BtsIMutI CAGTG 4 cut(s) 672, 816, 1001, 1044
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 422
CseI GACGC 1 cut(s) 725
Csp6I GTAC 4 cut(s) 199, 453, 852, 1030
CviAII CATG 1 cut(s) 900
CviQI GTAC 4 cut(s) 199, 453, 852, 1030
DdeI CTNAG 5 cut(s) 516, 614, 937, 985, 993
DpnI GATC 1 cut(s) 922
DpnII GATC 1 cut(s) 920
Eam1104I CTCTTC 3 cut(s) 115, 184, 705
EarI CTCTTC 3 cut(s) 115, 184, 705
Ecl136II GAGCTC 2 cut(s) 665, 784
Eco130I CCWWGG 1 cut(s) 418
Eco24I GRGCYC 2 cut(s) 667, 786
Eco47III AGCGCT 1 cut(s) 75
Eco53kI GAGCTC 2 cut(s) 665, 784
Eco57I CTGAAG 1 cut(s) 1020
EcoICRI GAGCTC 2 cut(s) 665, 784
EcoRII CCWGG 1 cut(s) 629
EcoT14I CCWWGG 1 cut(s) 418
EcoT38I GRGCYC 2 cut(s) 667, 786
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 1 cut(s) 903
FaiI YATR 9 cut(s) 14, 68, 80, 87, 89, 594, 609, 669, 901
FalI AAGNNNNNCTT 4 cut(s) 85, 117, 818, 850
FaqI GGGAC 1 cut(s) 887
FatI CATG 1 cut(s) 899
Fnu4HI GCNGC 8 cut(s) 6, 9, 31, 34, 350, 360, 582, 603
FokI GGATG 1 cut(s) 1013
FriOI GRGCYC 2 cut(s) 667, 786
Fsp4HI GCNGC 8 cut(s) 6, 9, 31, 34, 350, 360, 582, 603
FspBI CTAG 1 cut(s) 365
GlaI GCGC 1 cut(s) 75
GluI GCNGC 8 cut(s) 6, 9, 31, 34, 350, 360, 582, 603
GsuI CTGGAG 1 cut(s) 696
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 2 cut(s) 423, 893
HapII CCGG 2 cut(s) 942, 956
HgaI GACGC 1 cut(s) 725
HhaI GCGC 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 717
Hin1II CATG 1 cut(s) 903
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 1 cut(s) 715
HindII GTYRAC 1 cut(s) 715
HinfI GANTC 6 cut(s) 525, 842, 875, 932, 959, 989
HpaII CCGG 2 cut(s) 942, 956
HphI GGTGA 5 cut(s) 36, 271, 380, 903, 945
Hpy166II GTNNAC 1 cut(s) 715
Hpy188I TCNGA 5 cut(s) 58, 793, 964, 988, 1019
Hpy188III TCNNGA 1 cut(s) 918
Hpy8I GTNNAC 1 cut(s) 715
HpyAV CCTTC 3 cut(s) 144, 714, 1044
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 6 cut(s) 219, 365, 469, 578, 587, 716
HpyF3I CTNAG 5 cut(s) 516, 614, 937, 985, 993
Hsp92I GRCGYC 1 cut(s) 717
Hsp92II CATG 1 cut(s) 903
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 1 cut(s) 920
LmnI GCTCC 6 cut(s) 61, 373, 662, 729, 781, 826
Lsp1109I GCAGC 8 cut(s) 17, 20, 42, 45, 361, 371, 589, 593
LweI GCATC 1 cut(s) 664
MaeI CTAG 1 cut(s) 365
MaeIII GTNAC 1 cut(s) 42
MalI GATC 1 cut(s) 922
MboI GATC 1 cut(s) 920
MhlI GDGCHC 2 cut(s) 667, 786
MluCI AATT 7 cut(s) 291, 354, 637, 688, 732, 754, 884
MlyI GAGTC 3 cut(s) 519, 869, 941
MmeI TCCRAC 1 cut(s) 346
MseI TTAA 4 cut(s) 270, 651, 753, 1063
MspCI CTTAAG 1 cut(s) 650
MspI CCGG 2 cut(s) 942, 956
MspR9I CCNGG 2 cut(s) 631, 943
MvaI CCWGG 1 cut(s) 631
MwoI GCNNNNNNNGC 6 cut(s) 219, 365, 469, 578, 587, 716
NciI CCSGG 1 cut(s) 943
NdeII GATC 1 cut(s) 920
NlaIII CATG 1 cut(s) 903
NmuCI GTSAC 1 cut(s) 42
NspV TTCGAA 1 cut(s) 564
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PfeI GAWTC 3 cut(s) 842, 959, 989
PflMI CCANNNNNTGG 1 cut(s) 900
PkrI GCNGC 8 cut(s) 7, 10, 32, 35, 351, 361, 583, 604
PleI GAGTC 3 cut(s) 519, 869, 940
PpsI GAGTC 3 cut(s) 519, 869, 940
PsiI TTATAA 1 cut(s) 609
Psp124BI GAGCTC 2 cut(s) 667, 786
Psp6I CCWGG 1 cut(s) 629
PspGI CCWGG 1 cut(s) 629
PspPI GGNCC 1 cut(s) 422
RsaI GTAC 4 cut(s) 200, 454, 853, 1031
RsaNI GTAC 4 cut(s) 199, 453, 852, 1030
SacI GAGCTC 2 cut(s) 667, 786
SaqAI TTAA 4 cut(s) 270, 651, 753, 1063
SatI GCNGC 8 cut(s) 6, 9, 31, 34, 350, 360, 582, 603
Sau3AI GATC 1 cut(s) 920
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 3 cut(s) 519, 869, 941
ScrFI CCNGG 2 cut(s) 631, 943
SduI GDGCHC 2 cut(s) 667, 786
SfaNI GCATC 1 cut(s) 664
SfcI CTRYAG 2 cut(s) 12, 475
SfuI TTCGAA 1 cut(s) 564
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
Sse9I AATT 7 cut(s) 291, 354, 637, 688, 732, 754, 884
SsiI CCGC 1 cut(s) 708
SspMI CTAG 1 cut(s) 365
SstI GAGCTC 2 cut(s) 667, 786
StyD4I CCNGG 2 cut(s) 629, 941
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 564, 919
TasI AATT 7 cut(s) 291, 354, 637, 688, 732, 754, 884
TatI WGTACW 1 cut(s) 851
TfiI GAWTC 3 cut(s) 842, 959, 989
Tru1I TTAA 4 cut(s) 270, 651, 753, 1063
Tru9I TTAA 4 cut(s) 270, 651, 753, 1063
TscAI CASTG 4 cut(s) 679, 816, 1001, 1044
TseFI GTSAC 1 cut(s) 42
TseI GCWGC 8 cut(s) 5, 8, 30, 33, 349, 359, 581, 602
Tsp45I GTSAC 1 cut(s) 42
TspDTI ATGAA 5 cut(s) 104, 249, 455, 834, 888
TspRI CASTG 4 cut(s) 679, 816, 1001, 1044
Van91I CCANNNNNTGG 1 cut(s) 900
Vha464I CTTAAG 1 cut(s) 650
XapI RAATTY 1 cut(s) 291
XspI CTAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.