Rh2AG251200

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013978
Physical Location & Seq
Reverse (-)
28502810 .. 28505308
2499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG251200.1

Sequence Viewer

Length: 1053 bp
ATGGCAGCAGCTATAGATATTTACAGTAGCAGCAGCTCAATGTCACCAGATTTATCAGAGGAGCTTATGAGAGCACTTATGCCTTGTATGAACAGTGCTTCTTCAACCTCTTCTATTTCTCCACCAACATCTCCTTCCACTTCTTATTATTCTTCTTCTTCCTACACTCCATTTTACTCTTCTTCTTATCCCAATTTGTACCCTGACTTTTGCTCAAACACCCAAATGTTCCCACAAGTGGGTTTTGAGCAAACAGGTTCATTGGGGCTTAACCAACTCACCCCATCTCAAATTCGTCAAATCCAAACCCAAATGATACTCCAACAACAACAGAAGCAGCAAATTGCAGCTCTAGCTCCACTACCAAACCAATATCACCACCAACACTCTCACACCCTCAACTTCCTTGGCCCAAAAGCAATCCCAATGAAGCAATCTGGTACTCCCAAGCCCACAAAGCTCTACAGGGGAGTGAGGCAGAGACATTGGGGAAAATGGGTTGCTGAGATTAGACTCCCCAAGAACCGAACTCGCCTCTGGCTTGGCACATTCGAAACAGCCGAAGAAGCAGCTTTAGCTTATGATAAAGCTGCTTATAAGCTCAGGGGAGACTTTGCCAGGCTCAATTTCCCTCATCTTAAGCATCAAGGAGCTCATATCACTGGAGATTTTGGCAATTACAAGCCTCTTCCTTCCGCAGTTGACGCCAAGCTCCAAGCAATTTGCGAGAGCTTGGCTGTTAATTCGCCGAAACAGGGCAAAACAGAGGAGCTCTGTTCTGAAACAGAGACAAAGCCAATGATTTCAGCTCCAAAGATGGTCTTTGATGATTCATTGAGTACAGATTTGAAAAGAGAATGGGACTCTCCTAAATTGGAGGCCATTTCATGGTCATCTTCACCCTCTCGATCCTGCGATGAGTCCTCAGCCGGGTCATCTTCACCGGAATCTGACATTACATTCTTGGATTTCTCAGATTCTCAGTGGGATGAGAATGATACTTTCGGATTGGAGAAGTACCCTTCAGTGGAGATTGATTGGTCTGCTATTTAA

Protein Analysis

350

Amino Acids

38.95

Weight (kDa)

5.78

Isoelectric Point (pI)

60.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 154 - 203 3.1e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22200 AT4G39780
fragaria_vesca FvH4_1g21210
malus_domestica MD15G1326800.v1.1
prunus_persica Prupe.6G182200_v2.0.a1
pyrus_communis pycom01g05500 pycom395g00070
rosa_chinensis RchiOBHm_Chr2g0115041
rosa_laevigata RLG00000018150
rosa_multiflora Rmu_sc0000784.1_g000017
rosa_roxburghii Rroxscaffold_2G00130510
rosa_rugosa Rorug02G0192600
rosa_samantha Rh2AG251200 Rh2BG260700 Rh2CG255800 Rh2DG259200
rosa_wichuraiana Rw2G019610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 597
AccB7I CCANNNNNTGG 1 cut(s) 888
AciI CCGC 1 cut(s) 696
AclWI GGATC 1 cut(s) 903
AcsI RAATTY 1 cut(s) 291
AcuI CTGAAG 1 cut(s) 1008
AcyI GRCGYC 1 cut(s) 705
AfaI GTAC 4 cut(s) 200, 442, 841, 1019
AfiI CCNNNNNNNGG 6 cut(s) 238, 239, 755, 888, 930, 1027
AflII CTTAAG 1 cut(s) 638
AgsI TTSAA 2 cut(s) 105, 850
AjnI CCWGG 1 cut(s) 617
AjuI GAANNNNNNNTTGG 2 cut(s) 118, 150
Alw21I GWGCWC 3 cut(s) 76, 655, 774
Alw26I GTCTC 3 cut(s) 475, 603, 782
AlwI GGATC 1 cut(s) 903
AoxI GGCC 2 cut(s) 409, 879
ApeKI GCWGC 8 cut(s) 5, 8, 30, 33, 337, 347, 569, 590
ApoI RAATTY 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 410
AsuC2I CCSGG 1 cut(s) 931
AsuHPI GGTGA 5 cut(s) 36, 271, 368, 891, 933
AsuII TTCGAA 1 cut(s) 552
BanII GRGCYC 2 cut(s) 655, 774
Bbv12I GWGCWC 3 cut(s) 76, 655, 774
BbvCI CCTCAGC 1 cut(s) 925
BbvI GCAGC 8 cut(s) 17, 20, 42, 45, 349, 359, 577, 581
BccI CCATC 2 cut(s) 292, 811
BciT130I CCWGG 1 cut(s) 619
BcnI CCSGG 1 cut(s) 931
BcoDI GTCTC 3 cut(s) 475, 603, 782
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 2 cut(s) 12, 463
BfrI CTTAAG 1 cut(s) 638
BisI GCNGC 8 cut(s) 6, 9, 31, 34, 338, 348, 570, 591
BlsI GCNGC 8 cut(s) 7, 10, 32, 35, 339, 349, 571, 592
Bme1390I CCNGG 2 cut(s) 619, 931
BmgT120I GGNCC 1 cut(s) 410
BmrFI CCNGG 2 cut(s) 619, 931
BmsI GCATC 1 cut(s) 652
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 2 cut(s) 602, 925
Bpu14I TTCGAA 1 cut(s) 552
BpuMI CCSGG 1 cut(s) 931
BsaHI GRCGYC 1 cut(s) 705
BsaJI CCNNGG 1 cut(s) 406
BsaWI WCCGGW 1 cut(s) 943
Bsc4I CCNNNNNNNGG 6 cut(s) 238, 239, 755, 888, 930, 1027
Bse1I ACTGG 1 cut(s) 667
BseBI CCWGG 1 cut(s) 619
BseDI CCNNGG 1 cut(s) 406
BseGI GGATG 1 cut(s) 994
BseLI CCNNNNNNNGG 6 cut(s) 238, 239, 755, 888, 930, 1027
BseMII CTCAG 5 cut(s) 495, 616, 939, 987, 995
BseNI ACTGG 1 cut(s) 667
BseRI GAGGAG 2 cut(s) 74, 782
BseXI GCAGC 8 cut(s) 17, 20, 42, 45, 349, 359, 577, 581
BshFI GGCC 2 cut(s) 411, 881
BsiHKAI GWGCWC 3 cut(s) 76, 655, 774
BsiSI CCGG 2 cut(s) 930, 944
BslFI GGGAC 1 cut(s) 875
BslI CCNNNNNNNGG 6 cut(s) 238, 239, 755, 888, 930, 1027
BsmAI GTCTC 3 cut(s) 475, 603, 782
BsmFI GGGAC 1 cut(s) 875
BsnI GGCC 2 cut(s) 411, 881
Bsp119I TTCGAA 1 cut(s) 552
Bsp1286I GDGCHC 3 cut(s) 76, 655, 774
Bsp143I GATC 1 cut(s) 908
BspACI CCGC 1 cut(s) 696
BspANI GGCC 2 cut(s) 411, 881
BspCNI CTCAG 5 cut(s) 496, 615, 938, 986, 994
BspPI GGATC 1 cut(s) 903
BspT104I TTCGAA 1 cut(s) 552
BspTI CTTAAG 1 cut(s) 638
BsrI ACTGG 1 cut(s) 667
BssECI CCNNGG 1 cut(s) 406
BssMI GATC 1 cut(s) 908
BssNI GRCGYC 1 cut(s) 705
BssT1I CCWWGG 1 cut(s) 406
Bst2UI CCWGG 1 cut(s) 619
Bst4CI ACNGT 2 cut(s) 26, 95
Bst6I CTCTTC 3 cut(s) 115, 184, 693
BstACI GRCGYC 1 cut(s) 705
BstAFI CTTAAG 1 cut(s) 638
BstBI TTCGAA 1 cut(s) 552
BstDEI CTNAG 5 cut(s) 504, 602, 925, 973, 981
BstF5I GGATG 1 cut(s) 994
BstKTI GATC 1 cut(s) 911
BstMAI GTCTC 3 cut(s) 475, 603, 782
BstMBI GATC 1 cut(s) 908
BstMWI GCNNNNNNNGC 5 cut(s) 353, 457, 566, 575, 704
BstNI CCWGG 1 cut(s) 619
BstSCI CCNGG 2 cut(s) 617, 929
BstSFI CTRYAG 2 cut(s) 12, 463
BstV1I GCAGC 8 cut(s) 17, 20, 42, 45, 349, 359, 577, 581
BsuRI GGCC 2 cut(s) 411, 881
BtgZI GCGATG 1 cut(s) 930
BtsCI GGATG 1 cut(s) 994
BtsIMutI CAGTG 4 cut(s) 100, 660, 989, 1032
Cfr13I GGNCC 1 cut(s) 410
CseI GACGC 1 cut(s) 713
Csp6I GTAC 4 cut(s) 199, 441, 840, 1018
CviAII CATG 1 cut(s) 888
CviQI GTAC 4 cut(s) 199, 441, 840, 1018
DdeI CTNAG 5 cut(s) 504, 602, 925, 973, 981
DpnI GATC 1 cut(s) 910
DpnII GATC 1 cut(s) 908
Eam1104I CTCTTC 3 cut(s) 115, 184, 693
EarI CTCTTC 3 cut(s) 115, 184, 693
Ecl136II GAGCTC 2 cut(s) 653, 772
Eco130I CCWWGG 1 cut(s) 406
Eco24I GRGCYC 2 cut(s) 655, 774
Eco53kI GAGCTC 2 cut(s) 653, 772
Eco57I CTGAAG 1 cut(s) 1008
EcoICRI GAGCTC 2 cut(s) 653, 772
EcoRII CCWGG 1 cut(s) 617
EcoT14I CCWWGG 1 cut(s) 406
EcoT38I GRGCYC 2 cut(s) 655, 774
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 1 cut(s) 891
FaiI YATR 8 cut(s) 14, 68, 80, 89, 582, 597, 657, 889
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FaqI GGGAC 1 cut(s) 875
FatI CATG 1 cut(s) 887
Fnu4HI GCNGC 8 cut(s) 6, 9, 31, 34, 338, 348, 570, 591
FokI GGATG 1 cut(s) 1001
FriOI GRGCYC 2 cut(s) 655, 774
Fsp4HI GCNGC 8 cut(s) 6, 9, 31, 34, 338, 348, 570, 591
FspBI CTAG 1 cut(s) 353
GluI GCNGC 8 cut(s) 6, 9, 31, 34, 338, 348, 570, 591
GsuI CTGGAG 1 cut(s) 684
HaeIII GGCC 2 cut(s) 411, 881
HapII CCGG 2 cut(s) 930, 944
HgaI GACGC 1 cut(s) 713
Hin1I GRCGYC 1 cut(s) 705
Hin1II CATG 1 cut(s) 891
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HinfI GANTC 6 cut(s) 513, 830, 863, 920, 947, 977
HpaII CCGG 2 cut(s) 930, 944
HphI GGTGA 5 cut(s) 36, 271, 368, 891, 933
Hpy166II GTNNAC 1 cut(s) 703
Hpy188I TCNGA 5 cut(s) 58, 781, 952, 976, 1007
Hpy188III TCNNGA 1 cut(s) 906
Hpy8I GTNNAC 1 cut(s) 703
HpyAV CCTTC 3 cut(s) 144, 702, 1032
HpyCH4III ACNGT 2 cut(s) 26, 95
HpyCH4V TGCA 1 cut(s) 347
HpyF10VI GCNNNNNNNGC 5 cut(s) 353, 457, 566, 575, 704
HpyF3I CTNAG 5 cut(s) 504, 602, 925, 973, 981
Hsp92I GRCGYC 1 cut(s) 705
Hsp92II CATG 1 cut(s) 891
Kzo9I GATC 1 cut(s) 908
LmnI GCTCC 6 cut(s) 61, 361, 650, 717, 769, 814
Lsp1109I GCAGC 8 cut(s) 17, 20, 42, 45, 349, 359, 577, 581
LweI GCATC 1 cut(s) 652
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 42
MalI GATC 1 cut(s) 910
MboI GATC 1 cut(s) 908
MhlI GDGCHC 3 cut(s) 76, 655, 774
MluCI AATT 8 cut(s) 193, 291, 342, 625, 676, 720, 742, 872
MlyI GAGTC 3 cut(s) 507, 857, 929
MmeI TCCRAC 1 cut(s) 346
MseI TTAA 4 cut(s) 270, 639, 741, 1051
MslI CAYNNNNRTG 1 cut(s) 224
MspCI CTTAAG 1 cut(s) 638
MspI CCGG 2 cut(s) 930, 944
MspR9I CCNGG 2 cut(s) 619, 931
MvaI CCWGG 1 cut(s) 619
MwoI GCNNNNNNNGC 5 cut(s) 353, 457, 566, 575, 704
NciI CCSGG 1 cut(s) 931
NdeII GATC 1 cut(s) 908
NlaIII CATG 1 cut(s) 891
NmuCI GTSAC 1 cut(s) 42
NspV TTCGAA 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 558
PfeI GAWTC 3 cut(s) 830, 947, 977
PflMI CCANNNNNTGG 1 cut(s) 888
PkrI GCNGC 8 cut(s) 7, 10, 32, 35, 339, 349, 571, 592
PleI GAGTC 3 cut(s) 507, 857, 928
PpsI GAGTC 3 cut(s) 507, 857, 928
PsiI TTATAA 1 cut(s) 597
Psp124BI GAGCTC 2 cut(s) 655, 774
Psp6I CCWGG 1 cut(s) 617
PspGI CCWGG 1 cut(s) 617
PspPI GGNCC 1 cut(s) 410
RsaI GTAC 4 cut(s) 200, 442, 841, 1019
RsaNI GTAC 4 cut(s) 199, 441, 840, 1018
RseI CAYNNNNRTG 1 cut(s) 224
SacI GAGCTC 2 cut(s) 655, 774
SaqAI TTAA 4 cut(s) 270, 639, 741, 1051
SatI GCNGC 8 cut(s) 6, 9, 31, 34, 338, 348, 570, 591
Sau3AI GATC 1 cut(s) 908
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 3 cut(s) 507, 857, 929
ScrFI CCNGG 2 cut(s) 619, 931
SduI GDGCHC 3 cut(s) 76, 655, 774
SfaNI GCATC 1 cut(s) 652
SfcI CTRYAG 2 cut(s) 12, 463
SfuI TTCGAA 1 cut(s) 552
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
Sse9I AATT 8 cut(s) 193, 291, 342, 625, 676, 720, 742, 872
SsiI CCGC 1 cut(s) 696
SspMI CTAG 1 cut(s) 353
SstI GAGCTC 2 cut(s) 655, 774
StyD4I CCNGG 2 cut(s) 617, 929
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 2 cut(s) 26, 95
TaqI TCGA 2 cut(s) 552, 907
TasI AATT 8 cut(s) 193, 291, 342, 625, 676, 720, 742, 872
TatI WGTACW 1 cut(s) 839
TfiI GAWTC 3 cut(s) 830, 947, 977
Tru1I TTAA 4 cut(s) 270, 639, 741, 1051
Tru9I TTAA 4 cut(s) 270, 639, 741, 1051
TscAI CASTG 4 cut(s) 100, 667, 989, 1032
TseFI GTSAC 1 cut(s) 42
TseI GCWGC 8 cut(s) 5, 8, 30, 33, 337, 347, 569, 590
Tsp45I GTSAC 1 cut(s) 42
TspDTI ATGAA 5 cut(s) 104, 249, 443, 822, 876
TspRI CASTG 4 cut(s) 100, 667, 989, 1032
Van91I CCANNNNNTGG 1 cut(s) 888
Vha464I CTTAAG 1 cut(s) 638
XapI RAATTY 1 cut(s) 291
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.