pycom01g05500

DNA-binding domain in plant proteins such as APETALA2 and EREBPs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013978
Physical Location & Seq
Forward (+)
5221951 .. 5222922
972 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05500.1

Sequence Viewer

Length: 972 bp
ATGGCAAAAGCTATAGATCTTTACAGCAGCAGCACAATGACACCAGATTTATCAGATCTCTTGGGGGAAGATCAACTCATGAAGGCACTTACGCCTTTTATGAAAAGTGCTTCTTCAACATCTCCTTCCACTTCCTACAGTTACCCTCCTCAGAACAACCAAAACTTCAACTTATACTTCTGCTCATCACGTATGATTTCACAAGCAGGTGCTATAGGGCTTAACCAACTTATCCCATCTCAAATTCTTCAAATCCAATCCCAAATGATCCGCCAACAACACCAACAAAAGCAACAGATTTCTGCTTTTGCTTCTTCAGCTGCTTACCTTAAGCTGCTTAGTACAAAAGCAATCCCAATGAAGACAGCTGGTACTCCCCCCAAACTCACAAAGCTCTACAGGGGAGTGAGGCAGAGGCACTGGGGCAAATGGGTTGCTGAGATTAGACTCCCCAGGAACCGAAAACGTCTTTGGCTAGGTACATTTGACACCGCTGAAGAAGCTGCTTTGGCTTATGATAAAGCCGCTTTTAAGCTCAGGGGAGACTTTGCTCGCCTCAATTTCCCCCATCTTAAGCAACAAGGGGCTCATGTCTCCGGTGCCTTTGGCCGCTACAAGCCCCTTCATTCCTCCGTTGATGCGAAGCTCCAGGCGATTTGCCAGAGCTTGGCTGCTAATTCGATGAAACAGGGGAATACAGGGGAGCCCTGTTCTGTCGCAGAGACAAAGCCAGTGGTATCAGCTCCTTTAGAGACTAGGATGGATGATTCTTTCAAAACTGAATTAAAAAGTGAATGGGAGGCCTTTTCTTCGTCGTCATCTTCGCCTTTTTGGTCTGATCGGTCTGATGAGGCATCTACGCCGGAATCCGACATTACCTTCTTGGATTTCTCGGATTCTCAGTGGGATGAGGCTGAGAATTTTGGATTGGAGAAGTACCCTTCGGTGGAGATTGATTGGTCTGCTATCTAA

Protein Analysis

324

Amino Acids

35.98

Weight (kDa)

8.29

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 132 - 181 4.8e-16 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22200 AT4G39780
fragaria_vesca FvH4_1g21210
malus_domestica MD15G1326800.v1.1
prunus_persica Prupe.6G182200_v2.0.a1
pyrus_communis pycom01g05500 pycom395g00070
rosa_chinensis RchiOBHm_Chr2g0115041
rosa_laevigata RLG00000018150
rosa_multiflora Rmu_sc0000784.1_g000017
rosa_roxburghii Rroxscaffold_2G00130510
rosa_rugosa Rorug02G0192600
rosa_samantha Rh2AG251200 Rh2BG260700 Rh2CG255800 Rh2DG259200
rosa_wichuraiana Rw2G019610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 599
AccB7I CCANNNNNTGG 1 cut(s) 667
AciI CCGC 4 cut(s) 271, 492, 525, 610
AclWI GGATC 1 cut(s) 262
AcoI YGGCCR 1 cut(s) 607
AcsI RAATTY 2 cut(s) 243, 919
AcuI CTGAAG 2 cut(s) 300, 516
AfaI GTAC 4 cut(s) 343, 373, 481, 938
AfiI CCNNNNNNNGG 2 cut(s) 667, 946
AflII CTTAAG 2 cut(s) 329, 572
AgsI TTSAA 4 cut(s) 117, 169, 251, 775
AjnI CCWGG 2 cut(s) 452, 648
AjuI GAANNNNNNNTTGG 4 cut(s) 454, 486, 911, 943
Alw26I GTCTC 4 cut(s) 537, 598, 716, 746
AlwI GGATC 1 cut(s) 262
AoxI GGCC 2 cut(s) 607, 801
ApeKI GCWGC 6 cut(s) 27, 30, 320, 334, 503, 671
ApoI RAATTY 2 cut(s) 243, 919
BanI GGYRCC 1 cut(s) 599
BanII GRGCYC 2 cut(s) 589, 708
BbsI GAAGAC 1 cut(s) 368
BbvI GCAGC 6 cut(s) 39, 42, 307, 321, 490, 658
BccI CCATC 3 cut(s) 244, 576, 754
BciT130I CCWGG 2 cut(s) 454, 650
BcoDI GTCTC 4 cut(s) 537, 598, 716, 746
BfaI CTAG 2 cut(s) 476, 756
BfmI CTRYAG 4 cut(s) 12, 136, 213, 397
BfrI CTTAAG 2 cut(s) 329, 572
BfuAI ACCTGC 1 cut(s) 197
BglII AGATCT 2 cut(s) 16, 55
BisI GCNGC 8 cut(s) 28, 31, 321, 335, 504, 525, 610, 672
BlsI GCNGC 8 cut(s) 29, 32, 322, 336, 505, 526, 611, 673
Bme1390I CCNGG 2 cut(s) 454, 650
BmiI GGNNCC 3 cut(s) 458, 601, 705
BmrFI CCNGG 2 cut(s) 454, 650
BmrI ACTGGG 1 cut(s) 430
BmsI GCATC 2 cut(s) 628, 863
BmuI ACTGGG 1 cut(s) 430
BpiI GAAGAC 1 cut(s) 368
BpmI CTGGAG 1 cut(s) 632
Bpu10I CCTNAGC 1 cut(s) 536
BsaAI YACGTR 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 452
BsaWI WCCGGW 1 cut(s) 596
Bsc4I CCNNNNNNNGG 2 cut(s) 667, 946
Bse1I ACTGG 2 cut(s) 425, 731
BseBI CCWGG 2 cut(s) 454, 650
BseDI CCNNGG 1 cut(s) 452
BseGI GGATG 3 cut(s) 765, 769, 913
BseLI CCNNNNNNNGG 2 cut(s) 667, 946
BseMII CTCAG 5 cut(s) 164, 429, 550, 906, 914
BseNI ACTGG 2 cut(s) 425, 731
BseRI GAGGAG 1 cut(s) 138
BseXI GCAGC 6 cut(s) 39, 42, 307, 321, 490, 658
BshFI GGCC 2 cut(s) 609, 803
BshNI GGYRCC 1 cut(s) 599
BsiSI CCGG 2 cut(s) 597, 863
BslI CCNNNNNNNGG 2 cut(s) 667, 946
BsmAI GTCTC 4 cut(s) 537, 598, 716, 746
BsnI GGCC 2 cut(s) 609, 803
Bsp1286I GDGCHC 2 cut(s) 589, 708
Bsp143I GATC 5 cut(s) 16, 55, 70, 267, 838
BspACI CCGC 4 cut(s) 271, 492, 525, 610
BspANI GGCC 2 cut(s) 609, 803
BspCNI CTCAG 5 cut(s) 163, 430, 549, 907, 913
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 3 cut(s) 458, 601, 705
BspMI ACCTGC 1 cut(s) 197
BspPI GGATC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 599
BspTI CTTAAG 2 cut(s) 329, 572
BsrI ACTGG 2 cut(s) 425, 731
BssECI CCNNGG 1 cut(s) 452
BssMI GATC 5 cut(s) 16, 55, 70, 267, 838
Bst2UI CCWGG 2 cut(s) 454, 650
Bst4CI ACNGT 1 cut(s) 140
BstAFI CTTAAG 2 cut(s) 329, 572
BstBAI YACGTR 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 6 cut(s) 150, 338, 438, 536, 900, 915
BstF5I GGATG 3 cut(s) 765, 769, 913
BstKTI GATC 5 cut(s) 19, 58, 73, 270, 841
BstMAI GTCTC 4 cut(s) 537, 598, 716, 746
BstMBI GATC 5 cut(s) 16, 55, 70, 267, 838
BstMWI GCNNNNNNNGC 3 cut(s) 317, 500, 509
BstNI CCWGG 2 cut(s) 454, 650
BstSCI CCNGG 2 cut(s) 452, 648
BstSFI CTRYAG 4 cut(s) 12, 136, 213, 397
BstV1I GCAGC 6 cut(s) 39, 42, 307, 321, 490, 658
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 2 cut(s) 16, 55
BstYI RGATCY 2 cut(s) 16, 55
BsuRI GGCC 2 cut(s) 609, 803
BtsCI GGATG 3 cut(s) 765, 769, 913
BtsIMutI CAGTG 3 cut(s) 418, 738, 908
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 553
CciI TCATGA 1 cut(s) 78
Csp6I GTAC 4 cut(s) 342, 372, 480, 937
CspCI CAANNNNNGTGG 2 cut(s) 714, 749
CviAII CATG 2 cut(s) 79, 590
CviQI GTAC 4 cut(s) 342, 372, 480, 937
DdeI CTNAG 6 cut(s) 150, 338, 438, 536, 900, 915
DpnI GATC 5 cut(s) 18, 57, 72, 269, 840
DpnII GATC 5 cut(s) 16, 55, 70, 267, 838
EaeI YGGCCR 1 cut(s) 607
EciI GGCGGA 1 cut(s) 260
Eco147I AGGCCT 1 cut(s) 803
Eco24I GRGCYC 2 cut(s) 589, 708
Eco57I CTGAAG 2 cut(s) 300, 516
EcoRII CCWGG 2 cut(s) 452, 648
EcoT38I GRGCYC 2 cut(s) 589, 708
FaeI CATG 2 cut(s) 82, 593
FaiI YATR 8 cut(s) 14, 80, 101, 175, 194, 215, 516, 591
FalI AAGNNNNNCTT 2 cut(s) 97, 129
FatI CATG 2 cut(s) 78, 589
Fnu4HI GCNGC 8 cut(s) 28, 31, 321, 335, 504, 525, 610, 672
FokI GGATG 3 cut(s) 772, 776, 920
FriOI GRGCYC 2 cut(s) 589, 708
Fsp4HI GCNGC 8 cut(s) 28, 31, 321, 335, 504, 525, 610, 672
FspBI CTAG 2 cut(s) 476, 756
GluI GCNGC 8 cut(s) 28, 31, 321, 335, 504, 525, 610, 672
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 2 cut(s) 609, 803
HapII CCGG 2 cut(s) 597, 863
Hin1II CATG 2 cut(s) 82, 593
HinfI GANTC 4 cut(s) 447, 767, 866, 896
HpaII CCGG 2 cut(s) 597, 863
Hpy188I TCNGA 6 cut(s) 55, 153, 838, 847, 871, 895
Hpy188III TCNNGA 1 cut(s) 79
Hpy99I CGWCG 1 cut(s) 817
HpyAV CCTTC 5 cut(s) 76, 135, 632, 889, 951
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4IV ACGT 2 cut(s) 190, 466
HpyF10VI GCNNNNNNNGC 3 cut(s) 317, 500, 509
HpyF3I CTNAG 6 cut(s) 150, 338, 438, 536, 900, 915
HpySE526I ACGT 2 cut(s) 190, 466
Hsp92II CATG 2 cut(s) 82, 593
Kzo9I GATC 5 cut(s) 16, 55, 70, 267, 838
LmnI GCTCC 3 cut(s) 651, 703, 748
Lsp1109I GCAGC 6 cut(s) 39, 42, 307, 321, 490, 658
LweI GCATC 2 cut(s) 628, 863
MaeI CTAG 2 cut(s) 476, 756
MaeII ACGT 2 cut(s) 190, 466
MaeIII GTNAC 1 cut(s) 140
MalI GATC 5 cut(s) 18, 57, 72, 269, 840
MboI GATC 5 cut(s) 16, 55, 70, 267, 838
MboII GAAGA 8 cut(s) 80, 105, 239, 306, 373, 509, 801, 813
MflI RGATCY 2 cut(s) 16, 55
MhlI GDGCHC 2 cut(s) 589, 708
MluCI AATT 5 cut(s) 243, 559, 676, 782, 919
MlyI GAGTC 1 cut(s) 441
MmeI TCCRAC 1 cut(s) 894
MnlI CCTC 9 cut(s) 156, 159, 402, 408, 566, 640, 793, 844, 904
MseI TTAA 5 cut(s) 222, 330, 531, 573, 785
MspA1I CMGCKG 3 cut(s) 320, 368, 494
MspCI CTTAAG 2 cut(s) 329, 572
MspI CCGG 2 cut(s) 597, 863
MspR9I CCNGG 2 cut(s) 454, 650
MvaI CCWGG 2 cut(s) 454, 650
MwoI GCNNNNNNNGC 3 cut(s) 317, 500, 509
NdeII GATC 5 cut(s) 16, 55, 70, 267, 838
NlaIII CATG 2 cut(s) 82, 593
NlaIV GGNNCC 3 cut(s) 458, 601, 705
PagI TCATGA 1 cut(s) 78
PaqCI CACCTGC 1 cut(s) 197
PceI AGGCCT 1 cut(s) 803
PfeI GAWTC 3 cut(s) 767, 866, 896
PflMI CCANNNNNTGG 1 cut(s) 667
PkrI GCNGC 8 cut(s) 29, 32, 322, 336, 505, 526, 611, 673
PleI GAGTC 1 cut(s) 441
PpsI GAGTC 1 cut(s) 441
Ppu21I YACGTR 1 cut(s) 191
Psp6I CCWGG 2 cut(s) 452, 648
PspGI CCWGG 2 cut(s) 452, 648
PspN4I GGNNCC 3 cut(s) 458, 601, 705
PsuI RGATCY 2 cut(s) 16, 55
PvuII CAGCTG 2 cut(s) 320, 368
RsaI GTAC 4 cut(s) 343, 373, 481, 938
RsaNI GTAC 4 cut(s) 342, 372, 480, 937
SaqAI TTAA 5 cut(s) 222, 330, 531, 573, 785
SatI GCNGC 8 cut(s) 28, 31, 321, 335, 504, 525, 610, 672
Sau3AI GATC 5 cut(s) 16, 55, 70, 267, 838
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 2 cut(s) 454, 650
SduI GDGCHC 2 cut(s) 589, 708
SfaNI GCATC 2 cut(s) 628, 863
SfcI CTRYAG 4 cut(s) 12, 136, 213, 397
SmlI CTYRAG 2 cut(s) 329, 572
SmoI CTYRAG 2 cut(s) 329, 572
Sse9I AATT 5 cut(s) 243, 559, 676, 782, 919
SseBI AGGCCT 1 cut(s) 803
SsiI CCGC 4 cut(s) 271, 492, 525, 610
SspMI CTAG 2 cut(s) 476, 756
StuI AGGCCT 1 cut(s) 803
StyD4I CCNGG 2 cut(s) 452, 648
TaaI ACNGT 1 cut(s) 140
TaiI ACGT 2 cut(s) 193, 469
TaqI TCGA 1 cut(s) 680
TaqII GACCGA 1 cut(s) 831
TasI AATT 5 cut(s) 243, 559, 676, 782, 919
TatI WGTACW 1 cut(s) 341
TauI GCSGC 2 cut(s) 527, 612
TfiI GAWTC 3 cut(s) 767, 866, 896
Tru1I TTAA 5 cut(s) 222, 330, 531, 573, 785
Tru9I TTAA 5 cut(s) 222, 330, 531, 573, 785
TscAI CASTG 3 cut(s) 425, 738, 908
TseI GCWGC 6 cut(s) 27, 30, 320, 334, 503, 671
TspDTI ATGAA 5 cut(s) 95, 116, 374, 614, 698
TspGWI ACGGA 1 cut(s) 622
TspRI CASTG 3 cut(s) 425, 738, 908
Van91I CCANNNNNTGG 1 cut(s) 667
Vha464I CTTAAG 2 cut(s) 329, 572
XapI RAATTY 2 cut(s) 243, 919
XspI CTAG 2 cut(s) 476, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.