MD15G1338200.v1.1

Amino acid kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014351
Physical Location & Seq
Reverse (-)
38136265 .. 38140697
4433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1338200.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGGCGGCAATTTCGCCCTCATCCTTTGTCTCTGTATCCTCCTCCACCTCCTCCTGTTCGTTTTCATGTCCGTTTGTGAGTTGCTTGAAGCGCGCTTACGATGGCGTGTCGGTGAGTAATCACCGCTCACATGGGCGGTTGGCGGCGGTTAGCTGCTCATCTTCTGAATTGGGCTCCGGTTCCGACTCTGACTCCGTGAACCGCCGCAGTCATACTCAAATGTCATCTATGGCTCCCTTTGGGGTGACAATGAGTGGGAATGGCTCGTCTGGACCATCTTATAAGTGGCGAAGGGTTTTGCTTAAAGTAAGCGGAGAAGCACTTGCCGGGGATCATACACAGAATATTGATCCAAAGGTAACAATGGAAATTGCAAGGGAGGTTGCATCTGTTACTCGCCTTGGCATTGAGGTTGCAATTGTGGTTGGCGGGGGAAATATCTTCCGTGGATCTTCTTGGGTGGGAAGCAGTGGTCTTGACCAATCATCTGCTGATTACATCGGGATGCTGGCAACTGTCATGAACTCAATATTTCTTCAAGCAACAATGGAAAGTATCGGCATCCCTACTCGAGTGCAGACTGCTTTTCGCATGTCTGAGGTTGCAGAGCCTTATATACGTCGAAGAGCTGTAAGGCATTTGGAAAAGGGGAGGGTAGTGATCTTTGCAGCTGGAACTGGAAATCCGTTCTTCACCACAGATACTGCTGCAGCGCTTCGTGGTGCAGAAATCAATGCAGAGGTTGTGCTGAAAGCTACAAATGTTGATGGGGTTTACGACAACGATCCAAGGTGTGACCCGAATGCCCGTCTTCTTGAGAATCTAACATATCAAGAGGTGATGGCAAAAGATCTTTCAGTAATGGACATGACTGCCATTACCTTATGCCAGACAAACAAAATTCCTGTTGTTGTGTTCAATCTAAACAAGCCTGGTAACATCTCAAAAGCGATAAAGGGGGAGAAGGTTGGCACGTTAATTTGTAAGGAGGATAGTTTACCGCTGAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
AA_kinase

Protein Analysis

338

Amino Acids

35.9

Weight (kDa)

8.65

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_kinase PF00696 98 - 307 2.1e-36 Amino acid kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014351)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18680 AT3G18680
fragaria_vesca FvH4_1g24030
malus_domestica MD01G1011700.v1.1 MD15G1338200.v1.1
prunus_persica Prupe.6G157900_v2.0.a1 Prupe.6G157900_v2.0.a1
pyrus_communis pycom15g28830
rosa_chinensis RchiOBHm_Chr2g0120171
rosa_laevigata RLG00000018528
rosa_multiflora Rmu_sc0015469.1_g000003
rosa_roxburghii Rroxscaffold_2G00123650
rosa_rugosa Rorug02G0227300 Rorug02G0227400
rosa_samantha Rh2AG284000 Rh2DG309000
rosa_wichuraiana Rw2G022750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AccBSI CCGCTC 1 cut(s) 126
AccII CGCG 1 cut(s) 93
AclWI GGATC 4 cut(s) 339, 344, 457, 779
AcsI RAATTY 1 cut(s) 900
AfeI AGCGCT 1 cut(s) 714
AgsI TTSAA 3 cut(s) 88, 539, 919
AjnI CCWGG 1 cut(s) 931
AloI GAACNNNNNNTCC 2 cut(s) 667, 699
AluBI AGCT 5 cut(s) 153, 629, 671, 755, 1009
AluI AGCT 5 cut(s) 153, 629, 671, 755, 1009
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 4 cut(s) 339, 344, 457, 779
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 570
Aor51HI AGCGCT 1 cut(s) 714
ApeKI GCWGC 4 cut(s) 153, 668, 707, 710
ApoI RAATTY 1 cut(s) 900
AspLEI GCGC 3 cut(s) 93, 95, 715
AspS9I GGNCC 1 cut(s) 272
AsuC2I CCSGG 1 cut(s) 328
AsuHPI GGTGA 5 cut(s) 113, 124, 256, 685, 850
AvaI CYCGRG 1 cut(s) 570
AvaII GGWCC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 176
BarI GAAGNNNNNNTAC 2 cut(s) 80, 112
BbsI GAAGAC 1 cut(s) 803
BbvI GCAGC 4 cut(s) 140, 680, 694, 722
BccI CCATC 4 cut(s) 95, 283, 761, 835
BciT130I CCWGG 1 cut(s) 933
BciVI GTATCC 1 cut(s) 46
BcnI CCSGG 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 34
BfmI CTRYAG 1 cut(s) 708
BfoI RGCGCY 1 cut(s) 716
BfuI GTATCC 1 cut(s) 46
BglII AGATCT 1 cut(s) 850
BisI GCNGC 7 cut(s) 6, 144, 154, 205, 669, 708, 711
BlsI GCNGC 7 cut(s) 7, 145, 155, 206, 670, 709, 712
Bme1390I CCNGG 2 cut(s) 328, 933
Bme18I GGWCC 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 570
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 3 cut(s) 175, 181, 234
BmrFI CCNGG 2 cut(s) 328, 933
BmsI GCATC 3 cut(s) 395, 495, 570
BpiI GAAGAC 1 cut(s) 803
BpuEI CTTGAG 1 cut(s) 836
BpuMI CCSGG 1 cut(s) 328
BsaJI CCNNGG 4 cut(s) 327, 400, 445, 788
BsaWI WCCGGW 1 cut(s) 176
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bse1I ACTGG 1 cut(s) 682
BseBI CCWGG 1 cut(s) 933
BseDI CCNNGG 4 cut(s) 327, 400, 445, 788
BseGI GGATG 3 cut(s) 20, 510, 561
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 1 cut(s) 682
BsePI GCGCGC 1 cut(s) 91
BseRI GAGGAG 2 cut(s) 31, 40
BseXI GCAGC 4 cut(s) 140, 680, 694, 722
BsgI GTGCAG 2 cut(s) 596, 744
Bsh1236I CGCG 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 570
BsiSI CCGG 2 cut(s) 177, 327
BsmAI GTCTC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 808
BsoBI CYCGRG 1 cut(s) 570
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 6 cut(s) 331, 349, 449, 660, 784, 850
BspCNI CTCAG 1 cut(s) 589
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 1 cut(s) 519
BspLI GGNNCC 3 cut(s) 175, 181, 234
BspMAI CTGCAG 1 cut(s) 712
BspPI GGATC 4 cut(s) 339, 344, 457, 779
BspQI GCTCTTC 1 cut(s) 619
BsrBI CCGCTC 1 cut(s) 126
BsrI ACTGG 1 cut(s) 682
BssECI CCNNGG 4 cut(s) 327, 400, 445, 788
BssHII GCGCGC 1 cut(s) 91
BssMI GATC 6 cut(s) 331, 349, 449, 660, 784, 850
BssT1I CCWWGG 2 cut(s) 400, 788
Bst2UI CCWGG 1 cut(s) 933
Bst4CI ACNGT 1 cut(s) 517
Bst6I CTCTTC 1 cut(s) 619
BstC8I GCNNGC 2 cut(s) 93, 510
BstDEI CTNAG 1 cut(s) 597
BstDSI CCRYGG 1 cut(s) 445
BstF5I GGATG 3 cut(s) 20, 510, 561
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 716
BstHHI GCGC 3 cut(s) 93, 95, 715
BstKTI GATC 6 cut(s) 334, 352, 452, 663, 787, 853
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 6 cut(s) 331, 349, 449, 660, 784, 850
BstMWI GCNNNNNNNGC 1 cut(s) 90
BstNI CCWGG 1 cut(s) 933
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 2 cut(s) 326, 931
BstSFI CTRYAG 1 cut(s) 708
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 4 cut(s) 140, 680, 694, 722
BstV2I GAAGAC 1 cut(s) 803
BstX2I RGATCY 2 cut(s) 449, 850
BstYI RGATCY 2 cut(s) 449, 850
BsuI GTATCC 1 cut(s) 46
BtgI CCRYGG 1 cut(s) 445
BtsCI GGATG 3 cut(s) 20, 510, 561
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 475
Cac8I GCNNGC 2 cut(s) 93, 510
CaiI CAGNNNCTG 1 cut(s) 704
CciI TCATGA 1 cut(s) 519
CfoI GCGC 3 cut(s) 93, 95, 715
Cfr13I GGNCC 1 cut(s) 272
CviAII CATG 5 cut(s) 66, 131, 520, 592, 868
DdeI CTNAG 1 cut(s) 597
DpnI GATC 6 cut(s) 333, 351, 451, 662, 786, 852
DpnII GATC 6 cut(s) 331, 349, 449, 660, 784, 850
Eam1104I CTCTTC 1 cut(s) 619
EarI CTCTTC 1 cut(s) 619
Eco130I CCWWGG 2 cut(s) 400, 788
Eco24I GRGCYC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 272
Eco47III AGCGCT 1 cut(s) 714
Eco88I CYCGRG 1 cut(s) 570
EcoRII CCWGG 1 cut(s) 931
EcoT14I CCWWGG 2 cut(s) 400, 788
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 2 cut(s) 400, 788
FaeI CATG 5 cut(s) 69, 134, 523, 595, 871
FatI CATG 5 cut(s) 65, 130, 519, 591, 867
FauI CCCGC 1 cut(s) 422
Fnu4HI GCNGC 7 cut(s) 6, 144, 154, 205, 669, 708, 711
FokI GGATG 3 cut(s) 7, 517, 548
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 7 cut(s) 6, 144, 154, 205, 669, 708, 711
GlaI GCGC 3 cut(s) 92, 94, 714
GluI GCNGC 7 cut(s) 6, 144, 154, 205, 669, 708, 711
HaeII RGCGCY 1 cut(s) 716
HapII CCGG 2 cut(s) 177, 327
HhaI GCGC 3 cut(s) 93, 95, 715
Hin1II CATG 5 cut(s) 69, 134, 523, 595, 871
Hin6I GCGC 3 cut(s) 91, 93, 713
HinP1I GCGC 3 cut(s) 91, 93, 713
HinfI GANTC 3 cut(s) 185, 191, 820
HpaII CCGG 2 cut(s) 177, 327
HphI GGTGA 5 cut(s) 113, 124, 256, 685, 850
Hpy166II GTNNAC 3 cut(s) 199, 775, 998
Hpy188I TCNGA 4 cut(s) 166, 184, 190, 598
Hpy188III TCNNGA 6 cut(s) 270, 476, 502, 520, 815, 833
Hpy8I GTNNAC 3 cut(s) 199, 775, 998
Hpy99I CGWCG 1 cut(s) 624
HpyAV CCTTC 2 cut(s) 285, 958
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4IV ACGT 2 cut(s) 619, 974
HpyCH4V TGCA 9 cut(s) 374, 386, 416, 577, 605, 668, 710, 725, 737
HpyF10VI GCNNNNNNNGC 1 cut(s) 90
HpyF3I CTNAG 1 cut(s) 597
HpySE526I ACGT 2 cut(s) 619, 974
Hsp92II CATG 5 cut(s) 69, 134, 523, 595, 871
HspAI GCGC 3 cut(s) 91, 93, 713
Kzo9I GATC 6 cut(s) 331, 349, 449, 660, 784, 850
LguI GCTCTTC 1 cut(s) 619
LmnI GCTCC 2 cut(s) 179, 238
Lsp1109I GCAGC 4 cut(s) 140, 680, 694, 722
LweI GCATC 3 cut(s) 395, 495, 570
MaeII ACGT 2 cut(s) 619, 974
MaeIII GTNAC 5 cut(s) 244, 358, 391, 794, 935
MalI GATC 6 cut(s) 333, 351, 451, 662, 786, 852
MbiI CCGCTC 1 cut(s) 126
MboI GATC 6 cut(s) 331, 349, 449, 660, 784, 850
MboII GAAGA 7 cut(s) 153, 433, 444, 527, 636, 682, 803
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 2 cut(s) 449, 850
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 6 cut(s) 9, 167, 369, 417, 900, 978
MlyI GAGTC 2 cut(s) 179, 185
MmeI TCCRAC 1 cut(s) 207
MseI TTAA 2 cut(s) 303, 977
MslI CAYNNNNRTG 1 cut(s) 503
MspA1I CMGCKG 2 cut(s) 671, 1003
MspI CCGG 2 cut(s) 177, 327
MspR9I CCNGG 2 cut(s) 328, 933
MunI CAATTG 1 cut(s) 417
Mva1269I GAATGC 1 cut(s) 808
MvaI CCWGG 1 cut(s) 933
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 90
NciI CCSGG 1 cut(s) 328
NdeII GATC 6 cut(s) 331, 349, 449, 660, 784, 850
NlaIII CATG 5 cut(s) 69, 134, 523, 595, 871
NlaIV GGNNCC 3 cut(s) 175, 181, 234
NmuCI GTSAC 2 cut(s) 244, 794
NspI RCATGY 1 cut(s) 595
PaeR7I CTCGAG 1 cut(s) 570
PagI TCATGA 1 cut(s) 519
PauI GCGCGC 1 cut(s) 91
PciSI GCTCTTC 1 cut(s) 619
PctI GAATGC 1 cut(s) 808
PfeI GAWTC 1 cut(s) 820
PkrI GCNGC 7 cut(s) 7, 145, 155, 206, 670, 709, 712
PleI GAGTC 2 cut(s) 179, 185
PpsI GAGTC 2 cut(s) 179, 185
PsiI TTATAA 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 931
PspGI CCWGG 1 cut(s) 931
PspN4I GGNNCC 3 cut(s) 175, 181, 234
PspPI GGNCC 1 cut(s) 272
PspXI VCTCGAGB 1 cut(s) 570
PstI CTGCAG 1 cut(s) 712
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 2 cut(s) 449, 850
PteI GCGCGC 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 671
RseI CAYNNNNRTG 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 619
SaqAI TTAA 2 cut(s) 303, 977
SatI GCNGC 7 cut(s) 6, 144, 154, 205, 669, 708, 711
Sau3AI GATC 6 cut(s) 331, 349, 449, 660, 784, 850
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 2 cut(s) 179, 185
ScrFI CCNGG 2 cut(s) 328, 933
SduI GDGCHC 1 cut(s) 176
SfaNI GCATC 3 cut(s) 395, 495, 570
SfcI CTRYAG 1 cut(s) 708
Sfr274I CTCGAG 1 cut(s) 570
SinI GGWCC 1 cut(s) 272
SlaI CTCGAG 1 cut(s) 570
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 2 cut(s) 570, 815
SmoI CTYRAG 2 cut(s) 570, 815
Sse9I AATT 6 cut(s) 9, 167, 369, 417, 900, 978
SspI AATATT 2 cut(s) 346, 531
StyD4I CCNGG 2 cut(s) 326, 931
StyI CCWWGG 2 cut(s) 400, 788
TaaI ACNGT 1 cut(s) 517
TaiI ACGT 2 cut(s) 622, 977
TaqI TCGA 2 cut(s) 571, 622
TasI AATT 6 cut(s) 9, 167, 369, 417, 900, 978
TauI GCSGC 3 cut(s) 8, 146, 207
TfiI GAWTC 1 cut(s) 820
Tru1I TTAA 2 cut(s) 303, 977
Tru9I TTAA 2 cut(s) 303, 977
TscAI CASTG 1 cut(s) 475
TseFI GTSAC 2 cut(s) 244, 794
TseI GCWGC 4 cut(s) 153, 668, 707, 710
Tsp45I GTSAC 2 cut(s) 244, 794
TspDTI ATGAA 2 cut(s) 54, 536
TspGWI ACGGA 4 cut(s) 60, 184, 434, 675
TspRI CASTG 1 cut(s) 475
VpaK11BI GGWCC 1 cut(s) 272
XapI RAATTY 1 cut(s) 900
XceI RCATGY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 282
XhoI CTCGAG 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.