MD15G1366500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012941
Physical Location & Seq
Forward (+)
44515541 .. 44516668
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1366500.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGAAGCGAAGGGAGGCCAAAGGAGCACTCTCAGCTGATCTCTTAGTATGTTTTCCTTCAAGAACCAATCTAAAACTCATGCCGAAGCCAATTTGCAGTCCGGCAAGGCCCTCGGAGCCCAACTGCCGCCACCCCCACCACCACCAGCATCAACTCGTAAAAAAGTCGAGCATTGGGAGGAGCGGTCTTGGTCATGCTAGTCCTCTGATGTGGGCCAAGACCAAGTCCGTGGGTTCGGAGATCTCTGAGCCCACGTCCCCAAAAGTCACATGTGCTGGACAAATCAAAGTCCGGCACAAGTCAGGCTCGTGCAAAAGCTGGCAGTCAGTGATGGAACAGATTGAGAGAATTCACAACAACAAAAAACAGAGGAAACAGAGGAGACCCAATTGGGCTGAGGCTTTCGGGTTCAAGAAAGAAGTCATGCAATTCTTGACTTGCTTGAGAAGTATCAGGTTCGATTTTCGGTGTTTCGGTTCTTTTCCTCACGAATCCGATATTACTTCCGACGACGACGAAGACGAAGACGAAGAAGATGAGGAAGAAAACAGAGACGGTCGACAAAACCATGTGGGTGGTGTGGGAATTAGTGACGGTTCTGACCAGAGTAGCTCTAGTACTGTGTTTTCGAAATGGTTTATGGTTTTACAGGAAAATCAGAGTCATGGGTTTTGTAAAGAGGACAAAAAGGAGAGAAACTGTACGCCAAATTTCGATGACGGCGTCGGAATTGGATCAACTTCTGAAGCTCCAGCAGCCGCCGTTCCACCGCCAAATGCTTTGTTACTAATGAGGTGCAGGTCCGCCCCTGCAAAAACATGGCTAGAAGAGAAAGAAGAAGAAGACGACGACGAAGACGAAGAAGAAGAAGACGACGAAGAAGACGAAGAAGAAGAAGACGGCGACGATGAAGGAGGAGAAAATGGGTTGGAGAAGGAAGAGAAACAAGCAAAAGTTACACTGAGGAGTTTAATGGAGGAAGACAAGCAGAAAAAGAAGATGGAGAATTTGGTGGTGAGGGCATATGATTCTGATCTCTACAACATTTCATCAGACATTGCAAAGGAGACGTGGGTTGTTGGTGGATTGAAGGAAGCAATTTCCAGGAGTCGAAGTTGGAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

376

Amino Acids

42.43

Weight (kDa)

5.17

Isoelectric Point (pI)

70.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22230 AT1G78110
fragaria_vesca FvH4_5g37730
malus_domestica MD08G1180900.v1.1 MD15G1366500.v1.1
prunus_persica Prupe.1G514400_v2.0.a1
pyrus_communis pycom08g15460 pycom15g32860
rosa_chinensis RchiOBHm_Chr7g0239471
rosa_laevigata RLG00000000755
rosa_multiflora Rmu_sc0008652.1_g000005
rosa_roxburghii Rroxscaffold_3G00220920
rosa_rugosa Rorug07G0328100
rosa_samantha Rh7AG481600 Rh7BG454400 Rh7CG499600 Rh7DG467700
rosa_wichuraiana Rw7G040350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 789
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 559
AciI CCGC 5 cut(s) 127, 183, 759, 770, 804
AclWI GGATC 1 cut(s) 742
AcsI RAATTY 3 cut(s) 348, 709, 1006
AcuI CTGAAG 1 cut(s) 765
AcyI GRCGYC 1 cut(s) 723
AfaI GTAC 2 cut(s) 619, 703
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 3 cut(s) 60, 412, 1090
AjiI CACGTC 2 cut(s) 255, 1071
AjnI CCWGG 1 cut(s) 1103
AluBI AGCT 4 cut(s) 35, 318, 612, 749
AluI AGCT 4 cut(s) 35, 318, 612, 749
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 3 cut(s) 376, 546, 1061
AlwI GGATC 1 cut(s) 742
AoxI GGCC 3 cut(s) 15, 107, 213
ApeKI GCWGC 1 cut(s) 755
ApoI RAATTY 3 cut(s) 348, 709, 1006
AspS9I GGNCC 3 cut(s) 108, 213, 801
AsuHPI GGTGA 1 cut(s) 1027
AsuII TTCGAA 1 cut(s) 629
AvaII GGWCC 1 cut(s) 801
BanII GRGCYC 2 cut(s) 120, 252
BauI CACGAG 1 cut(s) 307
BbsI GAAGAC 8 cut(s) 525, 531, 849, 861, 876, 888, 903, 987
Bbv12I GWGCWC 1 cut(s) 28
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 767
BccI CCATC 2 cut(s) 325, 994
BceAI ACGGC 3 cut(s) 736, 746, 916
BcgI CGANNNNNNTGC 2 cut(s) 93, 127
BciT130I CCWGG 1 cut(s) 1105
BcoDI GTCTC 3 cut(s) 376, 546, 1061
BfaI CTAG 3 cut(s) 198, 615, 824
BfuAI ACCTGC 1 cut(s) 789
BglII AGATCT 1 cut(s) 240
BisI GCNGC 3 cut(s) 127, 756, 759
BlsI GCNGC 3 cut(s) 128, 757, 760
BmcAI AGTACT 1 cut(s) 619
Bme1390I CCNGG 1 cut(s) 1105
Bme18I GGWCC 1 cut(s) 801
BmgBI CACGTC 2 cut(s) 255, 1071
BmgT120I GGNCC 3 cut(s) 108, 213, 801
BmiI GGNNCC 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 1105
BmsI GCATC 1 cut(s) 157
BpiI GAAGAC 8 cut(s) 525, 531, 849, 861, 876, 888, 903, 987
BpmI CTGGAG 1 cut(s) 735
Bpu10I CCTNAGC 1 cut(s) 396
Bpu14I TTCGAA 1 cut(s) 629
BpuEI CTTGAG 1 cut(s) 463
BsaBI GATNNNNATC 1 cut(s) 1032
BsaHI GRCGYC 1 cut(s) 723
BsaI GGTCTC 1 cut(s) 376
BsaJI CCNNGG 2 cut(s) 111, 228
BsaXI ACNNNNNCTCC 3 cut(s) 169, 199, 1099
Bse3DI GCAATG 1 cut(s) 1056
Bse8I GATNNNNATC 1 cut(s) 1032
BseBI CCWGG 1 cut(s) 1105
BseDI CCNNGG 2 cut(s) 111, 228
BseJI GATNNNNATC 1 cut(s) 1032
BseMI GCAATG 1 cut(s) 1056
BseMII CTCAG 4 cut(s) 45, 237, 387, 953
BseRI GAGGAG 4 cut(s) 193, 394, 930, 979
BseXI GCAGC 1 cut(s) 767
BsgI GTGCAG 1 cut(s) 817
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 3 cut(s) 17, 109, 215
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 2 cut(s) 101, 292
BslFI GGGAC 1 cut(s) 241
BsmAI GTCTC 3 cut(s) 376, 546, 1061
BsmBI CGTCTC 2 cut(s) 546, 1061
BsmFI GGGAC 1 cut(s) 241
BsnI GGCC 3 cut(s) 17, 109, 215
Bso31I GGTCTC 1 cut(s) 376
Bsp119I TTCGAA 1 cut(s) 629
Bsp1286I GDGCHC 3 cut(s) 28, 120, 252
Bsp143I GATC 4 cut(s) 37, 240, 734, 1033
BspACI CCGC 5 cut(s) 127, 183, 759, 770, 804
BspANI GGCC 3 cut(s) 17, 109, 215
BspCNI CTCAG 4 cut(s) 44, 238, 388, 954
BspLI GGNNCC 1 cut(s) 117
BspMI ACCTGC 1 cut(s) 789
BspPI GGATC 1 cut(s) 742
BspT104I TTCGAA 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 376
BsrBI CCGCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 1056
BssECI CCNNGG 2 cut(s) 111, 228
BssMI GATC 4 cut(s) 37, 240, 734, 1033
BssNI GRCGYC 1 cut(s) 723
BssSI CACGAG 1 cut(s) 307
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 1105
Bst4CI ACNGT 4 cut(s) 557, 596, 622, 701
Bst6I CTCTTC 2 cut(s) 822, 933
BstACI GRCGYC 1 cut(s) 723
BstBI TTCGAA 1 cut(s) 629
BstC8I GCNNGC 1 cut(s) 320
BstDEI CTNAG 5 cut(s) 31, 43, 246, 396, 962
BstDSI CCRYGG 1 cut(s) 228
BstKTI GATC 4 cut(s) 40, 243, 737, 1036
BstMAI GTCTC 3 cut(s) 376, 546, 1061
BstMBI GATC 4 cut(s) 37, 240, 734, 1033
BstMCI CGRYCG 1 cut(s) 559
BstMWI GCNNNNNNNGC 4 cut(s) 23, 32, 115, 755
BstNI CCWGG 1 cut(s) 1105
BstNSI RCATGY 1 cut(s) 273
BstSCI CCNGG 1 cut(s) 1103
BstV1I GCAGC 1 cut(s) 767
BstV2I GAAGAC 8 cut(s) 525, 531, 849, 861, 876, 888, 903, 987
BstX2I RGATCY 1 cut(s) 240
BstXI CCANNNNNNTGG 2 cut(s) 229, 575
BstYI RGATCY 1 cut(s) 240
BsuRI GGCC 3 cut(s) 17, 109, 215
BtgI CCRYGG 1 cut(s) 228
BtrI CACGTC 2 cut(s) 255, 1071
BtsIMutI CAGTG 2 cut(s) 333, 959
BveI ACCTGC 1 cut(s) 789
Cac8I GCNNGC 1 cut(s) 320
Cfr13I GGNCC 3 cut(s) 108, 213, 801
CseI GACGC 1 cut(s) 712
Csp6I GTAC 2 cut(s) 618, 702
CviAII CATG 7 cut(s) 79, 194, 270, 424, 569, 665, 819
CviQI GTAC 2 cut(s) 618, 702
DdeI CTNAG 5 cut(s) 31, 43, 246, 396, 962
DpnI GATC 4 cut(s) 39, 242, 736, 1035
DpnII GATC 4 cut(s) 37, 240, 734, 1033
Eam1104I CTCTTC 2 cut(s) 822, 933
EarI CTCTTC 2 cut(s) 822, 933
EciI GGCGGA 1 cut(s) 793
Eco24I GRGCYC 2 cut(s) 120, 252
Eco31I GGTCTC 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 801
Eco57I CTGAAG 1 cut(s) 765
EcoO109I RGGNCCY 1 cut(s) 108
EcoRI GAATTC 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 1103
EcoT38I GRGCYC 2 cut(s) 120, 252
Esp3I CGTCTC 2 cut(s) 546, 1061
FaeI CATG 7 cut(s) 82, 197, 273, 427, 572, 668, 822
FaqI GGGAC 1 cut(s) 241
FatI CATG 7 cut(s) 78, 193, 269, 423, 568, 664, 818
FauNDI CATATG 1 cut(s) 1024
FblI GTMKAC 1 cut(s) 559
Fnu4HI GCNGC 3 cut(s) 127, 756, 759
FriOI GRGCYC 2 cut(s) 120, 252
Fsp4HI GCNGC 3 cut(s) 127, 756, 759
FspBI CTAG 3 cut(s) 198, 615, 824
GluI GCNGC 3 cut(s) 127, 756, 759
GsuI CTGGAG 1 cut(s) 735
HaeIII GGCC 3 cut(s) 17, 109, 215
HapII CCGG 2 cut(s) 101, 292
HgaI GACGC 1 cut(s) 712
Hin1I GRCGYC 1 cut(s) 723
Hin1II CATG 7 cut(s) 82, 197, 273, 427, 572, 668, 822
HincII GTYRAC 1 cut(s) 560
HindII GTYRAC 1 cut(s) 560
HinfI GANTC 4 cut(s) 491, 661, 1028, 1108
HpaII CCGG 2 cut(s) 101, 292
HphI GGTGA 1 cut(s) 1027
Hpy166II GTNNAC 1 cut(s) 560
Hpy188III TCNNGA 4 cut(s) 60, 412, 433, 488
Hpy8I GTNNAC 1 cut(s) 560
Hpy99I CGWCG 8 cut(s) 512, 515, 518, 728, 851, 854, 878, 908
HpyAV CCTTC 5 cut(s) 3, 66, 905, 928, 1084
HpyCH4III ACNGT 4 cut(s) 557, 596, 622, 701
HpyCH4IV ACGT 2 cut(s) 254, 1070
HpyCH4V TGCA 6 cut(s) 96, 312, 427, 798, 812, 1061
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 32, 115, 755
HpyF3I CTNAG 5 cut(s) 31, 43, 246, 396, 962
HpySE526I ACGT 2 cut(s) 254, 1070
Hsp92I GRCGYC 1 cut(s) 723
Hsp92II CATG 7 cut(s) 82, 197, 273, 427, 572, 668, 822
Kzo9I GATC 4 cut(s) 37, 240, 734, 1033
LmnI GCTCC 4 cut(s) 23, 115, 180, 754
Lsp1109I GCAGC 1 cut(s) 767
LweI GCATC 1 cut(s) 157
MaeI CTAG 3 cut(s) 198, 615, 824
MaeII ACGT 2 cut(s) 254, 1070
MaeIII GTNAC 4 cut(s) 265, 590, 783, 955
MalI GATC 4 cut(s) 39, 242, 736, 1035
MbiI CCGCTC 1 cut(s) 183
MboI GATC 4 cut(s) 37, 240, 734, 1033
MfeI CAATTG 1 cut(s) 388
MflI RGATCY 1 cut(s) 240
MhlI GDGCHC 3 cut(s) 28, 120, 252
MluCI AATT 9 cut(s) 90, 348, 388, 428, 585, 709, 729, 1006, 1098
MlyI GAGTC 2 cut(s) 670, 1117
MmeI TCCRAC 4 cut(s) 531, 706, 909, 1097
MseI TTAA 1 cut(s) 971
MslI CAYNNNNRTG 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 2 cut(s) 101, 292
MspR9I CCNGG 1 cut(s) 1105
MunI CAATTG 1 cut(s) 388
MvaI CCWGG 1 cut(s) 1105
MwoI GCNNNNNNNGC 4 cut(s) 23, 32, 115, 755
NdeI CATATG 1 cut(s) 1024
NdeII GATC 4 cut(s) 37, 240, 734, 1033
NlaIII CATG 7 cut(s) 82, 197, 273, 427, 572, 668, 822
NlaIV GGNNCC 1 cut(s) 117
NmuCI GTSAC 2 cut(s) 265, 590
NspI RCATGY 1 cut(s) 273
NspV TTCGAA 1 cut(s) 629
PciI ACATGT 1 cut(s) 269
PcsI WCGNNNNNNNCGW 4 cut(s) 519, 720, 855, 882
PfeI GAWTC 2 cut(s) 491, 1028
PflFI GACNNNGTC 2 cut(s) 223, 722
PfoI TCCNGGA 1 cut(s) 1103
PkrI GCNGC 3 cut(s) 128, 757, 760
PleI GAGTC 2 cut(s) 669, 1116
PpsI GAGTC 2 cut(s) 669, 1116
PscI ACATGT 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 1103
PspGI CCWGG 1 cut(s) 1103
PspN4I GGNNCC 1 cut(s) 117
PspPI GGNCC 3 cut(s) 108, 213, 801
PsuI RGATCY 1 cut(s) 240
PsyI GACNNNGTC 2 cut(s) 223, 722
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 2 cut(s) 619, 703
RsaNI GTAC 2 cut(s) 618, 702
RseI CAYNNNNRTG 1 cut(s) 573
SalI GTCGAC 1 cut(s) 558
SaqAI TTAA 1 cut(s) 971
SatI GCNGC 3 cut(s) 127, 756, 759
Sau3AI GATC 4 cut(s) 37, 240, 734, 1033
Sau96I GGNCC 3 cut(s) 108, 213, 801
ScaI AGTACT 1 cut(s) 619
SchI GAGTC 2 cut(s) 670, 1117
ScrFI CCNGG 1 cut(s) 1105
SduI GDGCHC 3 cut(s) 28, 120, 252
SetI ASST 9 cut(s) 37, 257, 320, 458, 614, 751, 797, 803, 1073
SfaNI GCATC 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 629
SinI GGWCC 1 cut(s) 801
SmiMI CAYNNNNRTG 1 cut(s) 573
SmlI CTYRAG 1 cut(s) 442
SmoI CTYRAG 1 cut(s) 442
Sse9I AATT 9 cut(s) 90, 348, 388, 428, 585, 709, 729, 1006, 1098
SsiI CCGC 5 cut(s) 127, 183, 759, 770, 804
SspMI CTAG 3 cut(s) 198, 615, 824
StyD4I CCNGG 1 cut(s) 1103
TaaI ACNGT 4 cut(s) 557, 596, 622, 701
TaiI ACGT 2 cut(s) 257, 1073
TaqI TCGA 6 cut(s) 167, 459, 559, 629, 714, 1111
TasI AATT 9 cut(s) 90, 348, 388, 428, 585, 709, 729, 1006, 1098
TatI WGTACW 1 cut(s) 617
TauI GCSGC 2 cut(s) 129, 761
TfiI GAWTC 2 cut(s) 491, 1028
Tru1I TTAA 1 cut(s) 971
Tru9I TTAA 1 cut(s) 971
TscAI CASTG 2 cut(s) 333, 966
TseFI GTSAC 2 cut(s) 265, 590
TseI GCWGC 1 cut(s) 755
Tsp45I GTSAC 2 cut(s) 265, 590
TspDTI ATGAA 3 cut(s) 17, 924, 1038
TspGWI ACGGA 1 cut(s) 217
TspRI CASTG 2 cut(s) 333, 966
Tth111I GACNNNGTC 2 cut(s) 223, 722
VpaK11BI GGWCC 1 cut(s) 801
XapI RAATTY 3 cut(s) 348, 709, 1006
XceI RCATGY 1 cut(s) 273
XmiI GTMKAC 1 cut(s) 559
XspI CTAG 3 cut(s) 198, 615, 824
ZrmI AGTACT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.