MD15G1368300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006218
Physical Location & Seq
Forward (+)
44801630 .. 44801988
359 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1368300.v1.1.491

Sequence Viewer

Length: 297 bp
ATGAGATCGGACGTCGCAATTCGCTGTGCAAAAGCCGCTTTTCTTCTCTCATCGCTCAAATCATCGCCCAATCGCCGCGTCAGCACCGCAATCGACGACGACGACGACGAAATCGCAGAGGAGAAGGAGATGCTGAAGAAGGAGATCGGAGATCTAAAGGTGACGGTTGCGAGGGAGCGACTCAGGAACAAGAGGATTAAGGTCTGTGGCTTGCTCGAGATTCTTCTTCAGCTGGCGTTGCTCTTTGCAATTTCCGCTTTCCTTTTCATGCTCGTGATTGAAGATGGAGAATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

99

Amino Acids

11.0

Weight (kDa)

5.5

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 15
AccII CGCG 1 cut(s) 78
AciI CCGC 4 cut(s) 36, 76, 87, 255
AcuI CTGAAG 2 cut(s) 155, 212
AcyI GRCGYC 1 cut(s) 12
AgsI TTSAA 1 cut(s) 281
AluBI AGCT 1 cut(s) 232
AluI AGCT 1 cut(s) 232
Ama87I CYCGRG 1 cut(s) 215
AsuHPI GGTGA 1 cut(s) 172
AvaI CYCGRG 1 cut(s) 215
BauI CACGAG 1 cut(s) 272
BccI CCATC 1 cut(s) 278
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BglII AGATCT 1 cut(s) 151
BisI GCNGC 2 cut(s) 36, 76
BlsI GCNGC 2 cut(s) 37, 77
BmeT110I CYCGRG 1 cut(s) 215
BmsI GCATC 1 cut(s) 120
BsaHI GRCGYC 1 cut(s) 12
BseMII CTCAG 1 cut(s) 196
BseRI GAGGAG 1 cut(s) 134
Bsh1236I CGCG 1 cut(s) 78
BsiHKCI CYCGRG 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 215
Bsp143I GATC 3 cut(s) 5, 144, 151
BspACI CCGC 4 cut(s) 36, 76, 87, 255
BspCNI CTCAG 1 cut(s) 195
BspFNI CGCG 1 cut(s) 78
BssMI GATC 3 cut(s) 5, 144, 151
BssNI GRCGYC 1 cut(s) 12
BssSI CACGAG 1 cut(s) 272
Bst2BI CACGAG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 166
BstACI GRCGYC 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 212, 234
BstDEI CTNAG 1 cut(s) 182
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 3 cut(s) 8, 147, 154
BstMBI GATC 3 cut(s) 5, 144, 151
BstMWI GCNNNNNNNGC 4 cut(s) 35, 81, 238, 254
BstUI CGCG 1 cut(s) 78
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BtgZI GCGATG 2 cut(s) 36, 48
Cac8I GCNNGC 2 cut(s) 212, 234
CseI GACGC 1 cut(s) 67
CviAII CATG 1 cut(s) 268
CviJI RGCY 3 cut(s) 35, 210, 232
CviKI_1 RGCY 3 cut(s) 35, 210, 232
DdeI CTNAG 1 cut(s) 182
DpnI GATC 3 cut(s) 7, 146, 153
DpnII GATC 3 cut(s) 5, 144, 151
Eco57I CTGAAG 2 cut(s) 155, 212
Eco88I CYCGRG 1 cut(s) 215
FaeI CATG 1 cut(s) 271
FaiI YATR 1 cut(s) 269
FatI CATG 1 cut(s) 267
Fnu4HI GCNGC 2 cut(s) 36, 76
Fsp4HI GCNGC 2 cut(s) 36, 76
GluI GCNGC 2 cut(s) 36, 76
HgaI GACGC 1 cut(s) 67
Hin1I GRCGYC 1 cut(s) 12
Hin1II CATG 1 cut(s) 271
HinfI GANTC 3 cut(s) 180, 220, 290
HphI GGTGA 1 cut(s) 172
Hpy188I TCNGA 2 cut(s) 10, 149
Hpy188III TCNNGA 3 cut(s) 184, 217, 274
Hpy99I CGWCG 6 cut(s) 17, 98, 101, 104, 107, 110
HpyAV CCTTC 2 cut(s) 118, 133
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 1 cut(s) 12
HpyCH4V TGCA 2 cut(s) 29, 248
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 81, 238, 254
HpyF3I CTNAG 1 cut(s) 182
HpySE526I ACGT 1 cut(s) 12
Hsp92I GRCGYC 1 cut(s) 12
Hsp92II CATG 1 cut(s) 271
Kzo9I GATC 3 cut(s) 5, 144, 151
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 2 cut(s) 169, 218
LweI GCATC 1 cut(s) 120
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 1 cut(s) 160
MalI GATC 3 cut(s) 7, 146, 153
MboI GATC 3 cut(s) 5, 144, 151
MboII GAAGA 5 cut(s) 35, 148, 215, 218, 293
MflI RGATCY 1 cut(s) 151
MluCI AATT 2 cut(s) 18, 249
MlyI GAGTC 1 cut(s) 174
MnlI CCTC 3 cut(s) 112, 165, 186
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 272
MspA1I CMGCKG 1 cut(s) 232
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 4 cut(s) 35, 81, 238, 254
NdeII GATC 3 cut(s) 5, 144, 151
NlaIII CATG 1 cut(s) 271
NmuCI GTSAC 1 cut(s) 160
PaeR7I CTCGAG 1 cut(s) 215
PcsI WCGNNNNNNNCGW 3 cut(s) 99, 102, 105
PfeI GAWTC 2 cut(s) 220, 290
PkrI GCNGC 2 cut(s) 37, 77
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
PsuI RGATCY 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 272
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 2 cut(s) 36, 76
Sau3AI GATC 3 cut(s) 5, 144, 151
SchI GAGTC 1 cut(s) 174
SetI ASST 4 cut(s) 15, 162, 204, 234
SfaNI GCATC 1 cut(s) 120
Sfr274I CTCGAG 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 2 cut(s) 18, 249
SsiI CCGC 4 cut(s) 36, 76, 87, 255
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 1 cut(s) 15
TaqI TCGA 2 cut(s) 93, 216
TasI AATT 2 cut(s) 18, 249
TauI GCSGC 2 cut(s) 38, 78
TfiI GAWTC 2 cut(s) 220, 290
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TseFI GTSAC 1 cut(s) 160
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 215
ZraI GACGTC 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.