MD16G1026500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005818
Physical Location & Seq
Forward (+)
1880028 .. 1882774
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1026500.v1.1.491

Sequence Viewer

Length: 753 bp
ATGAGTCCCCAAAGTACATTGAACCCGAGTAGAGCCCTAGAGGGCATGCATGGGGTGCATGTGGTGCCCTGTTCACGGCTTGAAATAGAAGAGACTACTGAAACTGGGGACCTTTCTCATTCAACTTGTGGAAGCTCAGCTATTGGAGGAAATCAGCAGCTTTTTATGCAGCGAGTGTGGCAGCAAAGACCGCCTTGTTTGAAGCCCATCCAGGGCTGCTGCATTCAAGGTGATCAGAGTGTTGCAGAAACCGTCGTGAATGTGCTCACTTCACTTCCTTTTATAGTTCTTGGAATCCACTCCCCAAGGAAGAATCTAAGTACCAAGCTATATGCTAATTCATTAATTGGAGTTGGAGTCGCCTCAAGTTTATATCATTCTTCGAGAGGAAAACTAAGGCAGTATTTAAGATGGGCCGATTATACAATGATAGCCACAACAACAGTATGTCTATCAAGAGCCCTCAGAAATGAAAACCCAAAACTGCTGATGGCAGCTTCTGCATTATGTTTACCCTTCCAGCCTCTGATGGTTTCAGCTGTTCATACTGGAATGATGGAGGTAGCATTCGCCAGGAGAGCATTGAAAGATCCTGAGCTAAGAAAGGCACACACTGTACATAAGATGTCGTCAATACTGGGAGGCGTGCTGTTCTTTGCCGACGACGTGTTTCCTCAGACTCCGTTCCTTCATGCTGCGTGGCACCTTGCTGCAGCCATCGGTGTTGGCACCTGCAATAAGCTTCTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

27.24

Weight (kDa)

8.98

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 740
Acc36I ACCTGC 1 cut(s) 740
AccB1I GGYRCC 3 cut(s) 64, 702, 728
AciI CCGC 1 cut(s) 191
AclWI GGATC 1 cut(s) 584
AfaI GTAC 3 cut(s) 16, 322, 618
AfiI CCNNNNNNNGG 2 cut(s) 75, 212
AflIII ACRYGT 1 cut(s) 666
AgsI TTSAA 6 cut(s) 22, 83, 123, 202, 227, 586
AjiI CACGTC 1 cut(s) 667
AjnI CCWGG 2 cut(s) 210, 572
AluBI AGCT 8 cut(s) 135, 140, 160, 328, 497, 539, 598, 742
AluI AGCT 8 cut(s) 135, 140, 160, 328, 497, 539, 598, 742
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 584
AlwNI CAGNNNCTG 2 cut(s) 500, 526
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 9 cut(s) 157, 169, 181, 216, 219, 494, 695, 710, 713
AseI ATTAAT 1 cut(s) 344
AspS9I GGNCC 2 cut(s) 109, 414
AsuHPI GGTGA 1 cut(s) 242
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 69
BanI GGYRCC 3 cut(s) 64, 702, 728
BanII GRGCYC 2 cut(s) 37, 463
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 9 cut(s) 169, 181, 193, 203, 206, 506, 682, 697, 725
BccI CCATC 6 cut(s) 215, 405, 484, 523, 550, 725
BceAI ACGGC 1 cut(s) 92
BciT130I CCWGG 2 cut(s) 212, 574
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 711
BfuAI ACCTGC 1 cut(s) 740
BisI GCNGC 9 cut(s) 158, 170, 182, 217, 220, 495, 696, 711, 714
BlpI GCTNAGC 1 cut(s) 136
BlsI GCNGC 9 cut(s) 159, 171, 183, 218, 221, 496, 697, 712, 715
Bme1390I CCNGG 2 cut(s) 212, 574
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 25
BmgBI CACGTC 1 cut(s) 667
BmgT120I GGNCC 2 cut(s) 109, 414
BmiI GGNNCC 4 cut(s) 66, 110, 704, 730
BmrFI CCNGG 2 cut(s) 212, 574
BmrI ACTGGG 2 cut(s) 114, 647
BmuI ACTGGG 2 cut(s) 114, 647
Bpu10I CCTNAGC 1 cut(s) 594
Bpu1102I GCTNAGC 1 cut(s) 136
BpuEI CTTGAG 1 cut(s) 349
BsaJI CCNNGG 2 cut(s) 211, 305
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 212
Bse1I ACTGG 3 cut(s) 109, 553, 642
BseBI CCWGG 2 cut(s) 212, 574
BseDI CCNNGG 2 cut(s) 211, 305
BseGI GGATG 1 cut(s) 207
BseLI CCNNNNNNNGG 2 cut(s) 75, 212
BseMII CTCAG 4 cut(s) 150, 478, 585, 689
BseNI ACTGG 3 cut(s) 109, 553, 642
BseSI GKGCMC 1 cut(s) 69
BseXI GCAGC 9 cut(s) 169, 181, 193, 203, 206, 506, 682, 697, 725
BshFI GGCC 1 cut(s) 416
BshNI GGYRCC 3 cut(s) 64, 702, 728
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 25
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 75, 212
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 122
BsmI GAATGC 2 cut(s) 222, 566
BsnI GGCC 1 cut(s) 416
BsoBI CYCGRG 1 cut(s) 25
Bsp1286I GDGCHC 4 cut(s) 37, 69, 267, 463
Bsp1407I TGTACA 1 cut(s) 616
Bsp143I GATC 2 cut(s) 232, 589
Bsp1720I GCTNAGC 1 cut(s) 136
BspACI CCGC 1 cut(s) 191
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 4 cut(s) 149, 477, 586, 688
BspLI GGNNCC 4 cut(s) 66, 110, 704, 730
BspMAI CTGCAG 1 cut(s) 715
BspMI ACCTGC 1 cut(s) 740
BspPI GGATC 1 cut(s) 584
BspT107I GGYRCC 3 cut(s) 64, 702, 728
BsrGI TGTACA 1 cut(s) 616
BsrI ACTGG 3 cut(s) 109, 553, 642
BssECI CCNNGG 2 cut(s) 211, 305
BssMI GATC 2 cut(s) 232, 589
BssT1I CCWWGG 1 cut(s) 305
Bst2UI CCWGG 2 cut(s) 212, 574
Bst4CI ACNGT 3 cut(s) 253, 445, 616
Bst6I CTCTTC 1 cut(s) 84
BstAPI GCANNNNNTGC 3 cut(s) 55, 64, 500
BstAUI TGTACA 1 cut(s) 616
BstC8I GCNNGC 2 cut(s) 47, 647
BstDEI CTNAG 7 cut(s) 136, 317, 395, 464, 594, 599, 675
BstF5I GGATG 1 cut(s) 207
BstKTI GATC 2 cut(s) 235, 592
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 2 cut(s) 232, 589
BstMWI GCNNNNNNNGC 7 cut(s) 55, 64, 166, 178, 190, 500, 578
BstNI CCWGG 2 cut(s) 212, 574
BstNSI RCATGY 2 cut(s) 49, 62
BstSCI CCNGG 2 cut(s) 210, 572
BstSFI CTRYAG 1 cut(s) 711
BstSLI GKGCMC 1 cut(s) 69
BstV1I GCAGC 9 cut(s) 169, 181, 193, 203, 206, 506, 682, 697, 725
BstX2I RGATCY 1 cut(s) 589
BstYI RGATCY 1 cut(s) 589
BsuRI GGCC 1 cut(s) 416
BtrI CACGTC 1 cut(s) 667
BtsCI GGATG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 612
BveI ACCTGC 1 cut(s) 740
Cac8I GCNNGC 2 cut(s) 47, 647
CaiI CAGNNNCTG 2 cut(s) 500, 526
Cfr13I GGNCC 2 cut(s) 109, 414
Csp6I GTAC 3 cut(s) 15, 321, 617
CviAII CATG 4 cut(s) 46, 50, 59, 692
CviQI GTAC 3 cut(s) 15, 321, 617
DdeI CTNAG 7 cut(s) 136, 317, 395, 464, 594, 599, 675
DpnI GATC 2 cut(s) 234, 591
DpnII GATC 2 cut(s) 232, 589
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 305
Eco24I GRGCYC 2 cut(s) 37, 463
Eco47I GGWCC 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 2 cut(s) 210, 572
EcoT14I CCWWGG 1 cut(s) 305
EcoT22I ATGCAT 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 37, 463
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 4 cut(s) 49, 53, 62, 695
FalI AAGNNNNNCTT 2 cut(s) 178, 210
FaqI GGGAC 1 cut(s) 122
FatI CATG 4 cut(s) 45, 49, 58, 691
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 9 cut(s) 158, 170, 182, 217, 220, 495, 696, 711, 714
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 37, 463
Fsp4HI GCNGC 9 cut(s) 158, 170, 182, 217, 220, 495, 696, 711, 714
FspBI CTAG 1 cut(s) 38
GluI GCNGC 9 cut(s) 158, 170, 182, 217, 220, 495, 696, 711, 714
HaeIII GGCC 1 cut(s) 416
Hin1II CATG 4 cut(s) 49, 53, 62, 695
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 5 cut(s) 4, 294, 313, 357, 679
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 2 cut(s) 74, 512
Hpy188I TCNGA 4 cut(s) 237, 467, 528, 678
Hpy188III TCNNGA 5 cut(s) 256, 384, 456, 593, 746
Hpy8I GTNNAC 2 cut(s) 74, 512
Hpy99I CGWCG 3 cut(s) 257, 665, 668
HpyAV CCTTC 2 cut(s) 526, 698
HpyCH4III ACNGT 3 cut(s) 253, 445, 616
HpyCH4IV ACGT 1 cut(s) 666
HpyCH4V TGCA 8 cut(s) 49, 58, 169, 222, 245, 503, 713, 735
HpyF10VI GCNNNNNNNGC 7 cut(s) 55, 64, 166, 178, 190, 500, 578
HpyF3I CTNAG 7 cut(s) 136, 317, 395, 464, 594, 599, 675
HpySE526I ACGT 1 cut(s) 666
Hsp92II CATG 4 cut(s) 49, 53, 62, 695
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 2 cut(s) 232, 589
Lsp1109I GCAGC 9 cut(s) 169, 181, 193, 203, 206, 506, 682, 697, 725
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 666
MalI GATC 2 cut(s) 234, 591
MboI GATC 2 cut(s) 232, 589
MboII GAAGA 3 cut(s) 101, 322, 372
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 4 cut(s) 37, 69, 267, 463
MluCI AATT 2 cut(s) 337, 345
MlyI GAGTC 3 cut(s) 13, 366, 673
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 9 cut(s) 34, 140, 373, 380, 473, 534, 553, 635, 684
Mph1103I ATGCAT 1 cut(s) 51
MseI TTAA 2 cut(s) 344, 407
MspA1I CMGCKG 1 cut(s) 539
MspR9I CCNGG 2 cut(s) 212, 574
Mva1269I GAATGC 2 cut(s) 222, 566
MvaI CCWGG 2 cut(s) 212, 574
MwoI GCNNNNNNNGC 7 cut(s) 55, 64, 166, 178, 190, 500, 578
NdeII GATC 2 cut(s) 232, 589
NlaIII CATG 4 cut(s) 49, 53, 62, 695
NlaIV GGNNCC 4 cut(s) 66, 110, 704, 730
NsiI ATGCAT 1 cut(s) 51
NspI RCATGY 2 cut(s) 49, 62
PaeI GCATGC 1 cut(s) 49
PaqCI CACCTGC 1 cut(s) 740
PctI GAATGC 2 cut(s) 222, 566
PfeI GAWTC 2 cut(s) 294, 313
PkrI GCNGC 9 cut(s) 159, 171, 183, 218, 221, 496, 697, 712, 715
PleI GAGTC 3 cut(s) 12, 365, 673
PpsI GAGTC 3 cut(s) 12, 365, 673
PpuMI RGGWCCY 1 cut(s) 109
PshBI ATTAAT 1 cut(s) 344
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 2 cut(s) 210, 572
PspGI CCWGG 2 cut(s) 210, 572
PspN4I GGNNCC 4 cut(s) 66, 110, 704, 730
PspPI GGNCC 2 cut(s) 109, 414
PspPPI RGGWCCY 1 cut(s) 109
PstI CTGCAG 1 cut(s) 715
PstNI CAGNNNCTG 2 cut(s) 500, 526
PsuI RGATCY 1 cut(s) 589
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 3 cut(s) 16, 322, 618
RsaNI GTAC 3 cut(s) 15, 321, 617
SaqAI TTAA 2 cut(s) 344, 407
SatI GCNGC 9 cut(s) 158, 170, 182, 217, 220, 495, 696, 711, 714
Sau3AI GATC 2 cut(s) 232, 589
Sau96I GGNCC 2 cut(s) 109, 414
SchI GAGTC 3 cut(s) 13, 366, 673
ScrFI CCNGG 2 cut(s) 212, 574
SduI GDGCHC 4 cut(s) 37, 69, 267, 463
SfcI CTRYAG 1 cut(s) 711
SinI GGWCC 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 364
SmoI CTYRAG 1 cut(s) 364
SphI GCATGC 1 cut(s) 49
Sse9I AATT 2 cut(s) 337, 345
SsiI CCGC 1 cut(s) 191
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 210, 572
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 3 cut(s) 253, 445, 616
TaiI ACGT 1 cut(s) 669
TaqI TCGA 1 cut(s) 383
TasI AATT 2 cut(s) 337, 345
TatI WGTACW 2 cut(s) 14, 616
TfiI GAWTC 2 cut(s) 294, 313
Tru1I TTAA 2 cut(s) 344, 407
Tru9I TTAA 2 cut(s) 344, 407
TscAI CASTG 1 cut(s) 619
TseI GCWGC 9 cut(s) 157, 169, 181, 216, 219, 494, 695, 710, 713
TspDTI ATGAA 4 cut(s) 330, 486, 533, 680
TspGWI ACGGA 1 cut(s) 672
TspRI CASTG 1 cut(s) 619
VpaK11BI GGWCC 1 cut(s) 109
VspI ATTAAT 1 cut(s) 344
XceI RCATGY 2 cut(s) 49, 62
XspI CTAG 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.