Rorug04G0334800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005818
Physical Location & Seq
Reverse (-)
50918424 .. 50920300
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0334800.1

Sequence Viewer

Length: 1500 bp
ATGGATTTCTTGAATTGTATGCTCTTATGTCTTTGCCTTGCCGGGCTCTCAGTCCAAGCCTTCCGTTCATTTGGAAGAAGCAAGGCAATTCCTAGAACTAGGCTTCCACCAGGACCAAAACCACTTCCATTAATTGGCAATCTCTTTGAGATTGGAGACAAACCCCATCTCTCTTTGACCAAGCTTTCCGAACGCTATGGCCCCATAGTCAGTTTGCAACTCGGTCAAGTAACCACAGTGGTGATTTCATCAGCATCCATGGCCAAAGAAATCCTCCGAACCCACGACCATCTCTTCTGCAACCGAACTATCCCAGATGCTGTCAATTCCTTCAAGCACTGCGAGGACAGCATCGTGTGGCTGCCTGTTTCAGACAAATGGAGAAACCTTCGCAAAATATGTAACTCGCAGCTGTTTGCCACCAAAGTTCTTGATGCAAACCAAGCCAACCGGCGCGTAAAAGTGCAAGAGCTCATTGCTGATATCTGCGAAAGCAAGGAGAAAGGTGTGGCAGTGAACATTGGAAGGGCTGCTTTCAAAACCACGCTCAATCTGCTGTCGCGGACTTTCTTCTCGGTGGATTTAGCTAACCCCAGTAGTGAGACGGCTAGGGAGTTGAAGGAGACTATTTGGGGTATCATGGAAGAGATAGGGAAACCAAACGTGGCCGACTATTTTCATTTTCTTAGAAAGCTTGACCCTCAAGGAATAAGGCGGCGGTTGACCTACCACTTTCACAAGACGATAGTCCTCTTTGACAGCATGATCAATCAAAGGTTGGAATCCAGAAAAGTGCAAGATTATATTCCGACTAATGATATGTTGGATACTCTTCTAAACATGAGTGAAGAGAAAAATGAGGAAATGGACAAGACCAAAGTTGAGCATTTGTTTCTGGACTTGATTGGTGCGGGGACAGATACAACTTCAGCCACAATGGAATGGGCAATGGCTGAGCTACTCTGCAGTCCAGAGAAACTTGCAAAAGCTCAAGCAGAGCTAGACCAAATAATTGGGAAAGGAAAACCAATTGAGGAATCAGACATTGCTCAACTCCCTTACTTGCAAGCAATAATCAAAGAAACATTTCGATTGCACCCAACAGTGCCATTGCTACTTCCCCGTAAAGCCAATTCAGAAGTAGAAATCGGAGGGTACATTATTCCTAAGGGTGCCCAAGTTCTGGTCAATGCTTGGGCCGTAGGCAGAGATTCCAGCGTTTGGGACAACCCAAGCTTGTTTACACCTGAGAGGTTTTTGGGGTCCGAAATTGATGTTATGGGAAGAAACTTCGAGCTTATTCCGTTTGGCGGTGGGAGGAGAATATGTCCAGGTTTGCCATTAGCGATGAGAATGTTGCACTTGATGTTGGGTTCAGTCCTGAACTGTTTTGATTGGAAGCTTGAAGATGGAGTTGTACCTGAGACTGTTAACATGGAAGACAAGTTTGGCCTCAGTTTACATATGGCTCATTCTCTGAGGGCTGTGCCCAACTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

499

Amino Acids

56.02

Weight (kDa)

7.18

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 36 - 482 3e-106 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1172
AccB7I CCANNNNNTGG 3 cut(s) 134, 1183, 1221
AccII CGCG 2 cut(s) 456, 562
AciI CCGC 5 cut(s) 562, 715, 718, 911, 1311
AcoI YGGCCR 2 cut(s) 261, 666
AcuI CTGAAG 1 cut(s) 912
AfaI GTAC 2 cut(s) 1157, 1419
AfiI CCNNNNNNNGG 4 cut(s) 134, 1183, 1221, 1310
AgsI TTSAA 5 cut(s) 13, 334, 538, 619, 1406
AjnI CCWGG 2 cut(s) 109, 1330
AjuI GAANNNNNNNTTGG 2 cut(s) 1431, 1463
AleI CACNNNNGTG 1 cut(s) 239
AloI GAACNNNNNNTCC 2 cut(s) 88, 120
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 4 cut(s) 150, 596, 617, 1418
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 5 cut(s) 199, 261, 666, 1197, 1450
ApeKI GCWGC 3 cut(s) 361, 409, 530
AseI ATTAAT 1 cut(s) 131
Asp700I GAANNNNTTC 1 cut(s) 1086
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 4 cut(s) 113, 200, 1197, 1263
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 253
AvaII GGWCC 2 cut(s) 113, 1263
AxyI CCTNAGG 1 cut(s) 1167
BaeGI GKGCMC 2 cut(s) 1177, 1491
BalI TGGCCA 1 cut(s) 263
BanI GGYRCC 1 cut(s) 1172
BanII GRGCYC 2 cut(s) 48, 474
BbsI GAAGAC 1 cut(s) 1446
Bbv12I GWGCWC 1 cut(s) 474
BbvI GCAGC 3 cut(s) 348, 421, 517
BccI CCATC 3 cut(s) 174, 297, 1403
BceAI ACGGC 2 cut(s) 621, 1184
BciT130I CCWGG 2 cut(s) 111, 1332
BciVI GTATCC 1 cut(s) 820
BclI TGATCA 1 cut(s) 765
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 4 cut(s) 150, 596, 617, 1418
BfaI CTAG 4 cut(s) 93, 99, 609, 1001
BfmI CTRYAG 1 cut(s) 964
BfuI GTATCC 1 cut(s) 820
BisI GCNGC 4 cut(s) 362, 410, 531, 716
BlpI GCTNAGC 1 cut(s) 954
BlsI GCNGC 4 cut(s) 363, 411, 532, 717
Bme1390I CCNGG 3 cut(s) 43, 111, 1332
Bme18I GGWCC 2 cut(s) 113, 1263
BmgT120I GGNCC 4 cut(s) 113, 200, 1197, 1263
BmiI GGNNCC 3 cut(s) 202, 1174, 1264
BmrFI CCNGG 3 cut(s) 43, 111, 1332
BmrI ACTGGG 1 cut(s) 588
BmsI GCATC 4 cut(s) 263, 307, 360, 424
BmuI ACTGGG 1 cut(s) 588
BoxI GACNNNNGTC 1 cut(s) 746
BpiI GAAGAC 1 cut(s) 1446
Bpu1102I GCTNAGC 1 cut(s) 954
BpuEI CTTGAG 2 cut(s) 687, 975
BpuMI CCSGG 1 cut(s) 43
BsaJI CCNNGG 1 cut(s) 258
BsaXI ACNNNNNCTCC 2 cut(s) 1312, 1342
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 1183, 1221, 1310
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 1 cut(s) 594
Bse21I CCTNAGG 1 cut(s) 1167
Bse3DI GCAATG 4 cut(s) 474, 954, 1044, 1109
BseBI CCWGG 2 cut(s) 111, 1332
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 254
BseLI CCNNNNNNNGG 4 cut(s) 134, 1183, 1221, 1310
BseMI GCAATG 4 cut(s) 474, 954, 1044, 1109
BseMII CTCAG 6 cut(s) 63, 945, 1239, 1413, 1468, 1469
BseNI ACTGG 1 cut(s) 594
BseRI GAGGAG 1 cut(s) 1333
BseSI GKGCMC 2 cut(s) 1177, 1491
BseXI GCAGC 3 cut(s) 348, 421, 517
Bsh1236I CGCG 2 cut(s) 456, 562
BshFI GGCC 5 cut(s) 201, 263, 668, 1199, 1452
BshNI GGYRCC 1 cut(s) 1172
BsiHKAI GWGCWC 1 cut(s) 474
BsiSI CCGG 2 cut(s) 42, 451
BslFI GGGAC 2 cut(s) 928, 1238
BslI CCNNNNNNNGG 4 cut(s) 134, 1183, 1221, 1310
BsmAI GTCTC 4 cut(s) 150, 596, 617, 1418
BsmBI CGTCTC 1 cut(s) 596
BsmFI GGGAC 2 cut(s) 928, 1238
BsnI GGCC 5 cut(s) 201, 263, 668, 1199, 1452
Bsp1286I GDGCHC 4 cut(s) 48, 474, 1177, 1491
Bsp143I GATC 1 cut(s) 765
Bsp1720I GCTNAGC 1 cut(s) 954
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 5 cut(s) 562, 715, 718, 911, 1311
BspANI GGCC 5 cut(s) 201, 263, 668, 1199, 1452
BspCNI CTCAG 6 cut(s) 62, 946, 1240, 1414, 1467, 1470
BspFNI CGCG 2 cut(s) 456, 562
BspLI GGNNCC 3 cut(s) 202, 1174, 1264
BspMAI CTGCAG 1 cut(s) 968
BspT107I GGYRCC 1 cut(s) 1172
BsrDI GCAATG 4 cut(s) 474, 954, 1044, 1109
BsrFI RCCGGY 1 cut(s) 450
BsrI ACTGG 1 cut(s) 594
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 1 cut(s) 765
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 2 cut(s) 111, 1332
Bst4CI ACNGT 4 cut(s) 238, 1105, 1388, 1429
Bst6I CTCTTC 4 cut(s) 299, 639, 837, 843
BstC8I GCNNGC 1 cut(s) 1068
BstDEI CTNAG 8 cut(s) 49, 686, 954, 1167, 1248, 1422, 1454, 1478
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 254
BstFNI CGCG 2 cut(s) 456, 562
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 1 cut(s) 768
BstMAI GTCTC 4 cut(s) 150, 596, 617, 1418
BstMBI GATC 1 cut(s) 765
BstMWI GCNNNNNNNGC 4 cut(s) 260, 348, 443, 553
BstNI CCWGG 2 cut(s) 111, 1332
BstPAI GACNNNNGTC 1 cut(s) 746
BstSCI CCNGG 3 cut(s) 41, 109, 1330
BstSFI CTRYAG 1 cut(s) 964
BstSLI GKGCMC 2 cut(s) 1177, 1491
BstUI CGCG 2 cut(s) 456, 562
BstV1I GCAGC 3 cut(s) 348, 421, 517
BstV2I GAAGAC 1 cut(s) 1446
BstXI CCANNNNNNTGG 1 cut(s) 1013
Bsu36I CCTNAGG 1 cut(s) 1167
BsuI GTATCC 1 cut(s) 820
BsuRI GGCC 5 cut(s) 201, 263, 668, 1199, 1452
BtgI CCRYGG 1 cut(s) 258
BtgZI GCGATG 1 cut(s) 1361
BtsCI GGATG 1 cut(s) 254
BtsI GCAGTG 2 cut(s) 337, 519
BtsIMutI CAGTG 4 cut(s) 243, 337, 519, 1110
Cac8I GCNNGC 1 cut(s) 1068
CaiI CAGNNNCTG 1 cut(s) 320
CfoI GCGC 1 cut(s) 456
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 4 cut(s) 113, 200, 1197, 1263
Csp6I GTAC 2 cut(s) 1156, 1418
CviAII CATG 5 cut(s) 259, 640, 763, 841, 1435
CviQI GTAC 2 cut(s) 1156, 1418
DdeI CTNAG 8 cut(s) 49, 686, 954, 1167, 1248, 1422, 1454, 1478
DpnI GATC 1 cut(s) 767
DpnII GATC 1 cut(s) 765
EaeI YGGCCR 2 cut(s) 261, 666
Eam1104I CTCTTC 4 cut(s) 299, 639, 837, 843
EarI CTCTTC 4 cut(s) 299, 639, 837, 843
Ecl136II GAGCTC 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 258
Eco24I GRGCYC 2 cut(s) 48, 474
Eco32I GATATC 1 cut(s) 484
Eco47I GGWCC 2 cut(s) 113, 1263
Eco53kI GAGCTC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 912
Eco81I CCTNAGG 1 cut(s) 1167
EcoICRI GAGCTC 1 cut(s) 472
EcoRII CCWGG 2 cut(s) 109, 1330
EcoRV GATATC 1 cut(s) 484
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 2 cut(s) 48, 474
ErhI CCWWGG 1 cut(s) 258
Esp3I CGTCTC 1 cut(s) 596
FaeI CATG 5 cut(s) 262, 643, 766, 844, 1438
FalI AAGNNNNNCTT 2 cut(s) 517, 549
FaqI GGGAC 2 cut(s) 928, 1238
FatI CATG 5 cut(s) 258, 639, 762, 840, 1434
FauI CCCGC 1 cut(s) 904
FauNDI CATATG 1 cut(s) 1464
FbaI TGATCA 1 cut(s) 765
Fnu4HI GCNGC 4 cut(s) 362, 410, 531, 716
FokI GGATG 1 cut(s) 241
FriOI GRGCYC 2 cut(s) 48, 474
Fsp4HI GCNGC 4 cut(s) 362, 410, 531, 716
FspBI CTAG 4 cut(s) 93, 99, 609, 1001
GlaI GCGC 1 cut(s) 455
GluI GCNGC 4 cut(s) 362, 410, 531, 716
HaeIII GGCC 5 cut(s) 201, 263, 668, 1199, 1452
HapII CCGG 2 cut(s) 42, 451
HhaI GCGC 1 cut(s) 456
Hin1II CATG 5 cut(s) 262, 643, 766, 844, 1438
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HincII GTYRAC 2 cut(s) 723, 1432
HindII GTYRAC 2 cut(s) 723, 1432
HindIII AAGCTT 4 cut(s) 182, 692, 1234, 1400
HinfI GANTC 3 cut(s) 782, 1037, 1211
HpaI GTTAAC 1 cut(s) 1432
HpaII CCGG 2 cut(s) 42, 451
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 5 cut(s) 517, 723, 1242, 1432, 1460
Hpy188I TCNGA 9 cut(s) 190, 278, 373, 810, 1042, 1138, 1151, 1267, 1479
Hpy188III TCNNGA 6 cut(s) 10, 431, 786, 896, 971, 1381
Hpy8I GTNNAC 5 cut(s) 517, 723, 1242, 1432, 1460
HpyAV CCTTC 5 cut(s) 70, 340, 398, 519, 613
HpyCH4III ACNGT 4 cut(s) 238, 1105, 1388, 1429
HpyCH4IV ACGT 1 cut(s) 663
HpyF10VI GCNNNNNNNGC 4 cut(s) 260, 348, 443, 553
HpyF3I CTNAG 8 cut(s) 49, 686, 954, 1167, 1248, 1422, 1454, 1478
HpySE526I ACGT 1 cut(s) 663
Hsp92II CATG 5 cut(s) 262, 643, 766, 844, 1438
HspAI GCGC 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 765
KspAI GTTAAC 1 cut(s) 1432
Kzo9I GATC 1 cut(s) 765
Lsp1109I GCAGC 3 cut(s) 348, 421, 517
LweI GCATC 4 cut(s) 263, 307, 360, 424
MaeI CTAG 4 cut(s) 93, 99, 609, 1001
MaeII ACGT 1 cut(s) 663
MaeIII GTNAC 2 cut(s) 229, 401
MalI GATC 1 cut(s) 767
MboI GATC 1 cut(s) 765
MboII GAAGA 9 cut(s) 87, 286, 562, 656, 824, 860, 1296, 1418, 1451
MfeI CAATTG 1 cut(s) 1029
MhlI GDGCHC 4 cut(s) 48, 474, 1177, 1491
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 8 cut(s) 13, 87, 132, 325, 1011, 1029, 1132, 1269
MluNI TGGCCA 1 cut(s) 263
MmeI TCCRAC 3 cut(s) 759, 804, 833
Mox20I TGGCCA 1 cut(s) 263
MroXI GAANNNNTTC 1 cut(s) 1086
MscI TGGCCA 1 cut(s) 263
MseI TTAA 2 cut(s) 131, 1431
MslI CAYNNNNRTG 1 cut(s) 239
Msp20I TGGCCA 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 2 cut(s) 42, 451
MspR9I CCNGG 3 cut(s) 43, 111, 1332
MunI CAATTG 1 cut(s) 1029
MvaI CCWGG 2 cut(s) 111, 1332
MvnI CGCG 2 cut(s) 456, 562
MwoI GCNNNNNNNGC 4 cut(s) 260, 348, 443, 553
NciI CCSGG 1 cut(s) 43
NcoI CCATGG 1 cut(s) 258
NdeI CATATG 1 cut(s) 1464
NdeII GATC 1 cut(s) 765
NlaIII CATG 5 cut(s) 262, 643, 766, 844, 1438
NlaIV GGNNCC 3 cut(s) 202, 1174, 1264
OliI CACNNNNGTG 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 1086
PfeI GAWTC 3 cut(s) 782, 1037, 1211
PflMI CCANNNNNTGG 3 cut(s) 134, 1183, 1221
PkrI GCNGC 4 cut(s) 363, 411, 532, 717
PshAI GACNNNNGTC 1 cut(s) 746
PshBI ATTAAT 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 474
Psp6I CCWGG 2 cut(s) 109, 1330
PspGI CCWGG 2 cut(s) 109, 1330
PspN4I GGNNCC 3 cut(s) 202, 1174, 1264
PspPI GGNCC 4 cut(s) 113, 200, 1197, 1263
PstI CTGCAG 1 cut(s) 968
PstNI CAGNNNCTG 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 412
RsaI GTAC 2 cut(s) 1157, 1419
RsaNI GTAC 2 cut(s) 1156, 1418
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 474
SaqAI TTAA 2 cut(s) 131, 1431
SatI GCNGC 4 cut(s) 362, 410, 531, 716
Sau3AI GATC 1 cut(s) 765
Sau96I GGNCC 4 cut(s) 113, 200, 1197, 1263
ScrFI CCNGG 3 cut(s) 43, 111, 1332
SduI GDGCHC 4 cut(s) 48, 474, 1177, 1491
SfaNI GCATC 4 cut(s) 263, 307, 360, 424
SfcI CTRYAG 1 cut(s) 964
SinI GGWCC 2 cut(s) 113, 1263
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 702, 990
SmoI CTYRAG 2 cut(s) 702, 990
Sse9I AATT 8 cut(s) 13, 87, 132, 325, 1011, 1029, 1132, 1269
SsiI CCGC 5 cut(s) 562, 715, 718, 911, 1311
SspMI CTAG 4 cut(s) 93, 99, 609, 1001
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 3 cut(s) 41, 109, 1330
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 4 cut(s) 238, 1105, 1388, 1429
TaiI ACGT 1 cut(s) 666
TaqI TCGA 2 cut(s) 1090, 1293
TaqII GACCGA 1 cut(s) 212
TasI AATT 8 cut(s) 13, 87, 132, 325, 1011, 1029, 1132, 1269
TauI GCSGC 1 cut(s) 718
TfiI GAWTC 3 cut(s) 782, 1037, 1211
Tru1I TTAA 2 cut(s) 131, 1431
Tru9I TTAA 2 cut(s) 131, 1431
TscAI CASTG 4 cut(s) 243, 344, 519, 1110
TseI GCWGC 3 cut(s) 361, 409, 530
TspDTI ATGAA 3 cut(s) 57, 237, 668
TspGWI ACGGA 2 cut(s) 53, 1293
TspRI CASTG 4 cut(s) 243, 344, 519, 1110
Van91I CCANNNNNTGG 3 cut(s) 134, 1183, 1221
VpaK11BI GGWCC 2 cut(s) 113, 1263
VspI ATTAAT 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 1086
XspI CTAG 4 cut(s) 93, 99, 609, 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.