pycom13g02220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005818
Physical Location & Seq
Forward (+)
1543926 .. 1545961
2036 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g02220.6

Sequence Viewer

Length: 750 bp
ATGAGTCCCAAAAGTACATTGAACTCGAGCAGAGCCCTACAGGGCATGCATGGGGTGCATGTGGTGTCCAGTTCTCGGTTTGAATCAGAAGAGACTACTCAAAATGACCTTTCTCACTCAACTTGTGGAACCTCACCTATTGGAGCAAATCAGCAGCTTTTTATGCAGCGAGTGTGGCAGCAAAGACCGCCATGCTTGAAGCCCATCCAGGGCTGCTGCATTCAAGGTGATCAGAGTGTTACAGAAACATTCGTGAATGTGCTCACTTCACTTCCTTTTATAGTTCTTGGAATCCATGCCCCAAGGAAGAATCTAAATACCAAGCTATATGCTAATTCATTAATCGGAGTTGGAGTCGCCTCAAGTTTATATCATTCTTCAAGAGGAAAACTAAGGAAATATTTAAGATGGGCCGACTATACGATGATAGCCACAACAACAGTATGTCTATCAAGAGCCCTCAGAAATGAAAACCCAAAGCTGCTGACGGCAGCTTCTGCATTATTTTTACCTTTCCAGCCTCTGATGGTTTCTGCCGTTCACACGGGATTAATGGAGGTAGCATTCGCCAAGAGAGCATTGAAAGATCCAGAGCTAAGAAATGCCCACTATGCACATAAGATGTCATCACTATTGGGAGGTGTCTTGTTCATTGCCGATGACATGTTTCCTCAGACTCCGTTCCTTCATGCTGCGTGGCACCTTGCTGCAGCCGTCGGTGTTGGCACCTGCAATAAGCTTCTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

27.28

Weight (kDa)

9.45

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 737
Acc36I ACCTGC 1 cut(s) 737
AccB1I GGYRCC 2 cut(s) 699, 725
AciI CCGC 1 cut(s) 188
AclWI GGATC 1 cut(s) 581
AfaI GTAC 1 cut(s) 16
AfiI CCNNNNNNNGG 2 cut(s) 75, 209
AflIII ACRYGT 1 cut(s) 663
AgsI TTSAA 6 cut(s) 22, 83, 199, 224, 381, 583
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 6 cut(s) 157, 325, 481, 494, 595, 739
AluI AGCT 6 cut(s) 157, 325, 481, 494, 595, 739
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 581
AlwNI CAGNNNCTG 2 cut(s) 497, 523
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 1 cut(s) 411
AseI ATTAAT 2 cut(s) 341, 551
AspS9I GGNCC 1 cut(s) 411
AsuHPI GGTGA 2 cut(s) 126, 239
AvaI CYCGRG 1 cut(s) 25
BanI GGYRCC 2 cut(s) 699, 725
BanII GRGCYC 2 cut(s) 37, 460
Bbv12I GWGCWC 1 cut(s) 264
BccI CCATC 3 cut(s) 212, 402, 520
BceAI ACGGC 3 cut(s) 504, 521, 698
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 86
BfmI CTRYAG 2 cut(s) 38, 708
BfuAI ACCTGC 1 cut(s) 737
Bme1390I CCNGG 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 411
BmiI GGNNCC 3 cut(s) 130, 701, 727
BmrFI CCNGG 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 346
BsaJI CCNNGG 2 cut(s) 208, 302
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 209
Bse1I ACTGG 1 cut(s) 69
Bse3DI GCAATG 1 cut(s) 651
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 2 cut(s) 208, 302
BseGI GGATG 1 cut(s) 204
BseLI CCNNNNNNNGG 2 cut(s) 75, 209
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 2 cut(s) 475, 686
BseNI ACTGG 1 cut(s) 69
BshFI GGCC 1 cut(s) 413
BshNI GGYRCC 2 cut(s) 699, 725
BsiHKAI GWGCWC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 25
BslI CCNNNNNNNGG 2 cut(s) 75, 209
BsmAI GTCTC 1 cut(s) 86
BsmI GAATGC 2 cut(s) 219, 563
BsnI GGCC 1 cut(s) 413
BsoBI CYCGRG 1 cut(s) 25
Bsp1286I GDGCHC 3 cut(s) 37, 264, 460
Bsp143I GATC 2 cut(s) 229, 586
BspACI CCGC 1 cut(s) 188
BspANI GGCC 1 cut(s) 413
BspCNI CTCAG 2 cut(s) 474, 685
BspLI GGNNCC 3 cut(s) 130, 701, 727
BspMAI CTGCAG 1 cut(s) 712
BspMI ACCTGC 1 cut(s) 737
BspPI GGATC 1 cut(s) 581
BspT107I GGYRCC 2 cut(s) 699, 725
BsrDI GCAATG 1 cut(s) 651
BsrI ACTGG 1 cut(s) 69
BssECI CCNNGG 2 cut(s) 208, 302
BssMI GATC 2 cut(s) 229, 586
BssT1I CCWWGG 1 cut(s) 302
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 1 cut(s) 84
BstAPI GCANNNNNTGC 2 cut(s) 55, 497
BstC8I GCNNGC 1 cut(s) 47
BstDEI CTNAG 4 cut(s) 392, 461, 596, 672
BstF5I GGATG 1 cut(s) 204
BstKTI GATC 2 cut(s) 232, 589
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 2 cut(s) 229, 586
BstMWI GCNNNNNNNGC 7 cut(s) 55, 163, 175, 187, 497, 575, 611
BstNI CCWGG 1 cut(s) 209
BstNSI RCATGY 3 cut(s) 49, 62, 667
BstSCI CCNGG 1 cut(s) 207
BstSFI CTRYAG 2 cut(s) 38, 708
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BsuRI GGCC 1 cut(s) 413
BtsCI GGATG 1 cut(s) 204
BveI ACCTGC 1 cut(s) 737
Cac8I GCNNGC 1 cut(s) 47
CaiI CAGNNNCTG 2 cut(s) 497, 523
Cfr13I GGNCC 1 cut(s) 411
Csp6I GTAC 1 cut(s) 15
CviAII CATG 7 cut(s) 46, 50, 59, 192, 296, 664, 689
CviQI GTAC 1 cut(s) 15
DdeI CTNAG 4 cut(s) 392, 461, 596, 672
DpnI GATC 2 cut(s) 231, 588
DpnII GATC 2 cut(s) 229, 586
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 302
Eco24I GRGCYC 2 cut(s) 37, 460
Eco88I CYCGRG 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 302
EcoT22I ATGCAT 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 37, 460
ErhI CCWWGG 1 cut(s) 302
FaeI CATG 7 cut(s) 49, 53, 62, 195, 299, 667, 692
FatI CATG 7 cut(s) 45, 49, 58, 191, 295, 663, 688
FbaI TGATCA 1 cut(s) 229
FokI GGATG 1 cut(s) 191
FriOI GRGCYC 2 cut(s) 37, 460
HaeIII GGCC 1 cut(s) 413
Hin1II CATG 7 cut(s) 49, 53, 62, 195, 299, 667, 692
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 6 cut(s) 4, 83, 291, 310, 354, 676
HphI GGTGA 2 cut(s) 126, 239
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 6 cut(s) 88, 234, 347, 464, 525, 675
Hpy188III TCNNGA 5 cut(s) 253, 381, 453, 590, 743
Hpy8I GTNNAC 1 cut(s) 541
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 1 cut(s) 695
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4V TGCA 8 cut(s) 49, 58, 166, 219, 500, 614, 710, 732
HpyF10VI GCNNNNNNNGC 7 cut(s) 55, 163, 175, 187, 497, 575, 611
HpyF3I CTNAG 4 cut(s) 392, 461, 596, 672
Hsp92II CATG 7 cut(s) 49, 53, 62, 195, 299, 667, 692
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 2 cut(s) 229, 586
LmnI GCTCC 1 cut(s) 143
LpnPI CCDG 8 cut(s) 26, 82, 194, 221, 530, 603, 728, 742
MaeIII GTNAC 1 cut(s) 238
MalI GATC 2 cut(s) 231, 588
MboI GATC 2 cut(s) 229, 586
MboII GAAGA 3 cut(s) 101, 319, 369
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 3 cut(s) 37, 264, 460
MluCI AATT 1 cut(s) 334
MlyI GAGTC 3 cut(s) 13, 363, 670
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 8 cut(s) 142, 370, 377, 470, 531, 550, 632, 681
Mph1103I ATGCAT 1 cut(s) 51
MseI TTAA 3 cut(s) 341, 404, 551
MspR9I CCNGG 1 cut(s) 209
Mva1269I GAATGC 2 cut(s) 219, 563
MvaI CCWGG 1 cut(s) 209
MwoI GCNNNNNNNGC 7 cut(s) 55, 163, 175, 187, 497, 575, 611
NdeII GATC 2 cut(s) 229, 586
NlaIII CATG 7 cut(s) 49, 53, 62, 195, 299, 667, 692
NlaIV GGNNCC 3 cut(s) 130, 701, 727
NsiI ATGCAT 1 cut(s) 51
NspI RCATGY 3 cut(s) 49, 62, 667
PaeI GCATGC 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 25
PaqCI CACCTGC 1 cut(s) 737
PciI ACATGT 1 cut(s) 663
PctI GAATGC 2 cut(s) 219, 563
PfeI GAWTC 3 cut(s) 83, 291, 310
PleI GAGTC 3 cut(s) 12, 362, 670
PpsI GAGTC 3 cut(s) 12, 362, 670
PscI ACATGT 1 cut(s) 663
PshBI ATTAAT 2 cut(s) 341, 551
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 3 cut(s) 130, 701, 727
PspPI GGNCC 1 cut(s) 411
PspXI VCTCGAGB 1 cut(s) 25
PstI CTGCAG 1 cut(s) 712
PstNI CAGNNNCTG 2 cut(s) 497, 523
PsuI RGATCY 1 cut(s) 586
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
SaqAI TTAA 3 cut(s) 341, 404, 551
Sau3AI GATC 2 cut(s) 229, 586
Sau96I GGNCC 1 cut(s) 411
SchI GAGTC 3 cut(s) 13, 363, 670
ScrFI CCNGG 1 cut(s) 209
SduI GDGCHC 3 cut(s) 37, 264, 460
SfcI CTRYAG 2 cut(s) 38, 708
Sfr274I CTCGAG 1 cut(s) 25
SlaI CTCGAG 1 cut(s) 25
SmlI CTYRAG 2 cut(s) 25, 361
SmoI CTYRAG 2 cut(s) 25, 361
SphI GCATGC 1 cut(s) 49
Sse9I AATT 1 cut(s) 334
SsiI CCGC 1 cut(s) 188
SspI AATATT 1 cut(s) 401
StyD4I CCNGG 1 cut(s) 207
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 1 cut(s) 442
TaqI TCGA 1 cut(s) 26
TasI AATT 1 cut(s) 334
TatI WGTACW 1 cut(s) 14
TfiI GAWTC 3 cut(s) 83, 291, 310
Tru1I TTAA 3 cut(s) 341, 404, 551
Tru9I TTAA 3 cut(s) 341, 404, 551
TspDTI ATGAA 4 cut(s) 327, 483, 640, 677
TspGWI ACGGA 1 cut(s) 669
VspI ATTAAT 2 cut(s) 341, 551
XceI RCATGY 3 cut(s) 49, 62, 667
XhoI CTCGAG 1 cut(s) 25
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.