MD16G1034800.v1.1

Pentatricopeptide repeat-containing protein At1g22960

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011481
Physical Location & Seq
Forward (+)
2482415 .. 2485418
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1034800.v1.1.491

Sequence Viewer

Length: 2178 bp
ATGACCCTCTGCGCCAGAGCTTCAAAAGCTTTAGCCGCCACTGCCTCCTCCTCCTCCGCCGTACACCACCGTCCTCTCAAGGTACGCCATTTCTTCGCCCTCTCTGGGTCTTCCCAGAACTGCTCCTCAATTACTTCTCTACGCACCACAGCTTTCTCCCAAACCCATTACCGGGACCTTATTTTCGACACCATTGACGAAAAGCCCTGGGCTTTTTGCAACTCCAAGTGGGTCTCCGATCGTTTCCAAGCTGTTATTGTCGACCCCGACTTGTTTGTTCGAGTCCTCGTCGAGATTAGGACCCGACCCAGAATCGCTCTGAGGTTTTTCCGATGGGTCGAGGGCCAACCGGGTTTTAAAAGATCCGAGTTTGCGTTCTGTGTGATTCTTGAGATTCTGGCTCACAACAATTTGATGAGGCCGGCGCATTGGGTTATGGAGAGGGTGATTAGTGTAAATATGCATGGGATTGTGGATGTTTTGATTAATGAGTATGTGTTTTCGAAGGTCTCGTTGAAGCTTCTCGATCTCTTGTTCTGGGTTTATACAAAAAAATCGATGCTTGAGCAATGTCTCTGGATTTTCGATAAGATGGTTCGAAACCGGTTGTTGCCAGACGTGAAGAACTGCAATAGGGTTCTTAGGATACTTAGAAATAAACACCTTGTGACTAGAGTTAAAGACGTTTACAGAATGATGGGCGAGGCTGGGATTAAGCCGACGATTGTTACCTATAATACTATGTTGGATTCATTCTGCAGGGAAGGAGAAGTGCAACAAGCACTTGACCTTTTGTCAGAGATGCAAAAGATAGAGTGTTTTCCCAACGATGTAACGTATAATGTGTTGATCAATGGGCTGTCAAAGAAAGGGGAGCTGGAGCAGGCCAAGGGGCTGATCAAGGAAATGATGAAATCTGGATTGAGGATTACAGCATTTACTTATAACCCTTTGATTTGTGGGTATTGCAACAAAGGACTGCTTGAAGAGGCATTATGTCTTGAGAAAGAAATGGTAATTAGAGGAGCGAATCCCACCGTGGCTACTTATAATTCGTTAATGTATGGACTCTGCAAGCGGGGAAGAGTGACTGATGCCAGGGAACAGTTTTCCAATATGTTAAAGAGAAATATAAGGCCGGATATAGTTTCATACAACACTCTAATATACGGGTATTGTAGATTAGGGAACTTGGGGGATGCTTTCAGATTGTTTGATGAGTTGAGGCATAGAACTTTCACTCCTACTCTCGTCACCTATAACACACTTATGGATGGTCTTTGCAGATCCGGAGACTTGGCTGTTGCTCGACAGCTTAAAAAAGAAATGACCAATCAAGGAACTTGCCCTGATGTTTTTACATATACCATCTTGGTAAATGGATCTTGCAAGGCGGGAAACTTATCGATGGCCAAGGAGTTATTTGATGAGATGTTGCATAGAGGAGTGGAGCCGGACCGATTTGCGTACAATACTCGAATAGTGGGTGAATTGACGCTTGGTGACCCTTCCAAGGCATTTAGCATGCAAGAAGAAATACTGGCAGGGGGTTTCCCTCCTGATTTGTTCACATACAATATTTTTGTGAATGGGATTTGTAAATTGGGCAATTTGGATGAAGCATATACCTTGTTGCAGAAAATGGTACGTGATGGTATCGTTCCAGACCATATAACTTATACTAGCATCATTCACGCTCACCTGGAGAGTGGCCAACTCATGAAGGCAAGAGAGGTGTTTTATGAGATGCTTGGCAAAGGTTTGTCTCCTTCGGTGATAACTTACACAGTATTGATTCATGCACACGCGGCTAAGGGAAGACTAGAACTGGCATATATGTACTTCTCAGAGATGCAAGAGAAGCATATTTTGCCAAATGTGGTCACCTACAATGCGCTGATAAATGGCCTTTGTAAAGTGATGAGAATGGATCAGGCTTACAAGTCGTTTACTGAGATGGAGGAGAAAGGAATAGCTCCAAATAAGTATACATACACTATTCTAATAAATGAGAACTGCAACATAGGGAACTGGAAGGAGGCTTTGAGGTTGTATAAACAGATGCTTGACAGAGAGGTCGAGCCTGATTCTTGTACACACAGTGCACTTTTTAAGCATCTAGACAAAGACTATCAGTTGCATGCAGTTCGGTACCTTGAGAATTTAATTTCAAGCTAG

Protein Analysis

726

Amino Acids

83.01

Weight (kDa)

8.75

Isoelectric Point (pI)

30.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 194 - 252 7.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 205 - 254 3.5e-07 PPR repeat family
TPR_24 PF23276 211 - 308 4.3e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 231 - 285 8.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 237 - 269 2.3e-12 PPR repeat
PPR_2 PF13041 240 - 289 1.3e-18 PPR repeat family
PPR PF01535 243 - 271 6.6e-08 PPR repeat
PPR_long PF17177 266 - 359 1.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 273 - 303 1.1e-08 PPR repeat
PPR_2 PF13041 275 - 321 2.6e-12 PPR repeat family
PPR PF01535 278 - 308 4.5e-08 PPR repeat
TPR_24 PF23276 299 - 407 1.8e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 307 - 339 2.2e-07 PPR repeat
PPR_2 PF13041 312 - 359 8e-14 PPR repeat family
PPR_1 PF12854 341 - 373 1.1e-07 PPR repeat
PPR_2 PF13041 345 - 389 3.5e-13 PPR repeat family
PPR_3 PF13812 371 - 425 1.2e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 376 - 408 2.4e-12 PPR repeat
PPR_2 PF13041 380 - 429 1.8e-17 PPR repeat family
PPR PF01535 383 - 410 2.4e-07 PPR repeat
PPR_1 PF12854 413 - 444 1.2e-09 PPR repeat
PPR_2 PF13041 415 - 464 2.5e-16 PPR repeat family
PPR_3 PF13812 439 - 492 1.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 447 - 478 5e-12 PPR repeat
PPR_2 PF13041 450 - 492 1.3e-12 PPR repeat family
PPR PF01535 453 - 483 1.1e-07 PPR repeat
PPR_2 PF13041 488 - 534 3.1e-07 PPR repeat family
PPR_1 PF12854 517 - 549 8.6e-11 PPR repeat
PPR_2 PF13041 520 - 566 4e-13 PPR repeat family
PPR PF01535 523 - 553 1.6e-06 PPR repeat
PPR_3 PF13812 544 - 599 5.2e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 555 - 603 1.6e-12 PPR repeat family
PPR_long PF17177 574 - 687 5.5e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 582 - 635 3.4e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 586 - 619 9.7e-06 PPR repeat
PPR_2 PF13041 590 - 639 6e-17 PPR repeat family
PPR_1 PF12854 622 - 654 8.4e-12 PPR repeat
PPR_2 PF13041 625 - 674 8.3e-20 PPR repeat family
PPR PF01535 628 - 658 6.6e-07 PPR repeat
PPR_3 PF13812 650 - 705 5.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 656 - 688 1.7e-07 PPR repeat
PPR_2 PF13041 668 - 707 5.8e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22960 AT1G22960
fragaria_vesca FvH4_4g26410
malus_domestica MD13G1032800.v1.1 MD16G1034800.v1.1
pyrus_communis pycom13g02830 pycom16g02960
rosa_chinensis RchiOBHm_Chr4g0434041
rosa_laevigata RLG00000006706
rosa_multiflora Rmu_co8498505.1_g000001 Rmu_sc0000352.1_g000026
rosa_roxburghii Rroxscaffold_5G00375450
rosa_rugosa Rorug04G0272900
rosa_samantha Rh4AG328600 Rh4BG335900 Rh4CG351400 Rh4DG332300 Rh5AG231100 Rh5BG086200
rosa_wichuraiana Rw4G028490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 945, 1050
AasI GACNNNNNNGTC 1 cut(s) 2075
Acc65I GGTACC 1 cut(s) 2151
AccB1I GGYRCC 1 cut(s) 2151
AccI GTMKAC 2 cut(s) 261, 1988
AccII CGCG 1 cut(s) 1808
AccIII TCCGGA 1 cut(s) 1289
AciI CCGC 5 cut(s) 36, 57, 1078, 1394, 1808
AclWI GGATC 4 cut(s) 357, 1281, 1390, 1938
AcoI YGGCCR 2 cut(s) 1410, 1711
AcsI RAATTY 1 cut(s) 2161
AdeI CACNNNGTG 2 cut(s) 667, 2102
AfaI GTAC 7 cut(s) 63, 84, 1469, 1647, 1841, 2095, 2153
AfiI CCNNNNNNNGG 4 cut(s) 105, 171, 172, 1513
AgeI ACCGGT 1 cut(s) 603
AgsI TTSAA 4 cut(s) 24, 517, 986, 2172
AhdI GACNNNNNGTC 1 cut(s) 793
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 3 cut(s) 206, 1097, 1701
AjuI GAANNNNNNNTTGG 2 cut(s) 1482, 1514
AloI GAACNNNNNNTCC 2 cut(s) 1225, 1257
AluBI AGCT 9 cut(s) 20, 29, 152, 251, 520, 877, 1315, 1976, 2175
AluI AGCT 9 cut(s) 20, 29, 152, 251, 520, 877, 1315, 1976, 2175
Alw21I GWGCWC 1 cut(s) 2107
Alw26I GTCTC 5 cut(s) 238, 514, 578, 1287, 1770
Alw44I GTGCAC 1 cut(s) 2103
AlwI GGATC 4 cut(s) 357, 1281, 1390, 1938
Aor13HI TCCGGA 1 cut(s) 1289
AoxI GGCC 7 cut(s) 343, 419, 885, 1136, 1410, 1711, 1906
ApaLI GTGCAC 1 cut(s) 2103
ApoI RAATTY 1 cut(s) 2161
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AseI ATTAAT 1 cut(s) 486
AsiGI ACCGGT 1 cut(s) 603
Asp718I GGTACC 1 cut(s) 2151
AspLEI GCGC 3 cut(s) 14, 427, 1897
AspS9I GGNCC 4 cut(s) 175, 300, 343, 1456
AsuC2I CCSGG 2 cut(s) 173, 351
AsuHPI GGTGA 7 cut(s) 457, 1246, 1499, 1514, 1691, 1786, 1876
AsuII TTCGAA 2 cut(s) 503, 598
AvaII GGWCC 3 cut(s) 175, 300, 1456
BaeGI GKGCMC 1 cut(s) 2107
BalI TGGCCA 2 cut(s) 1412, 1713
BanI GGYRCC 1 cut(s) 2151
BbsI GAAGAC 2 cut(s) 102, 1825
Bbv12I GWGCWC 1 cut(s) 2107
BccI CCATC 8 cut(s) 327, 586, 691, 1268, 1376, 1402, 1646, 1951
BceAI ACGGC 1 cut(s) 44
BcgI CGANNNNNNTGC 2 cut(s) 2129, 2163
BciT130I CCWGG 3 cut(s) 208, 1099, 1703
BciVI GTATCC 1 cut(s) 639
BclI TGATCA 2 cut(s) 849, 897
BcnI CCSGG 2 cut(s) 173, 351
BcoDI GTCTC 5 cut(s) 238, 514, 578, 1287, 1770
BfaI CTAG 5 cut(s) 672, 1683, 1823, 2120, 2176
BfmI CTRYAG 1 cut(s) 757
BfuI GTATCC 1 cut(s) 639
BisI GCNGC 2 cut(s) 36, 1809
BlsI GCNGC 2 cut(s) 37, 1810
Bme1390I CCNGG 5 cut(s) 173, 208, 351, 1099, 1703
Bme18I GGWCC 3 cut(s) 175, 300, 1456
BmeRI GACNNNNNGTC 1 cut(s) 793
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 4 cut(s) 175, 300, 343, 1456
BmiI GGNNCC 4 cut(s) 176, 302, 1452, 2153
BmrFI CCNGG 5 cut(s) 173, 208, 351, 1099, 1703
BmsI GCATC 9 cut(s) 549, 792, 1084, 1189, 1695, 1737, 1842, 2052, 2125
BpiI GAAGAC 2 cut(s) 102, 1825
BpmI CTGGAG 2 cut(s) 899, 1724
Bpu10I CCTNAGC 1 cut(s) 1812
Bpu14I TTCGAA 2 cut(s) 503, 598
BpuEI CTTGAG 5 cut(s) 62, 410, 584, 1022, 2177
BpuMI CCSGG 2 cut(s) 173, 351
Bsa29I ATCGAT 2 cut(s) 557, 1406
BsaAI YACGTR 1 cut(s) 1649
BsaI GGTCTC 2 cut(s) 238, 514
BsaJI CCNNGG 7 cut(s) 206, 207, 888, 1038, 1098, 1413, 1512
BsaWI WCCGGW 2 cut(s) 603, 1289
BsaXI ACNNNNNCTCC 2 cut(s) 1225, 1255
Bsc4I CCNNNNNNNGG 4 cut(s) 105, 171, 172, 1513
Bse118I RCCGGY 2 cut(s) 421, 603
Bse1I ACTGG 3 cut(s) 1545, 1833, 2036
Bse3DI GCAATG 1 cut(s) 575
BseAI TCCGGA 1 cut(s) 1289
BseBI CCWGG 3 cut(s) 208, 1099, 1703
BseCI ATCGAT 2 cut(s) 557, 1406
BseDI CCNNGG 7 cut(s) 206, 207, 888, 1038, 1098, 1413, 1512
BseGI GGATG 4 cut(s) 481, 1204, 1279, 1621
BseLI CCNNNNNNNGG 4 cut(s) 105, 171, 172, 1513
BseMI GCAATG 1 cut(s) 575
BseMII CTCAG 3 cut(s) 311, 1860, 1944
BseNI ACTGG 3 cut(s) 1545, 1833, 2036
BseRI GAGGAG 7 cut(s) 37, 40, 43, 115, 1038, 1458, 1976
BseSI GKGCMC 1 cut(s) 2107
BseYI CCCAGC 1 cut(s) 707
Bsh1236I CGCG 1 cut(s) 1808
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 7 cut(s) 345, 421, 887, 1138, 1412, 1713, 1908
BshNI GGYRCC 1 cut(s) 2151
BshTI ACCGGT 1 cut(s) 603
BshVI ATCGAT 2 cut(s) 557, 1406
BsiEI CGRYCG 1 cut(s) 241
BsiHKAI GWGCWC 1 cut(s) 2107
BsiSI CCGG 7 cut(s) 172, 350, 422, 604, 1139, 1290, 1454
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 4 cut(s) 105, 171, 172, 1513
BsmAI GTCTC 5 cut(s) 238, 514, 578, 1287, 1770
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 7 cut(s) 345, 421, 887, 1138, 1412, 1713, 1908
Bso31I GGTCTC 2 cut(s) 238, 514
Bsp119I TTCGAA 2 cut(s) 503, 598
Bsp1286I GDGCHC 1 cut(s) 2107
Bsp13I TCCGGA 1 cut(s) 1289
Bsp1407I TGTACA 1 cut(s) 2093
Bsp143I GATC 8 cut(s) 238, 362, 526, 849, 897, 1286, 1382, 1930
BspACI CCGC 5 cut(s) 36, 57, 1078, 1394, 1808
BspANI GGCC 7 cut(s) 345, 421, 887, 1138, 1412, 1713, 1908
BspCNI CTCAG 3 cut(s) 312, 1859, 1945
BspDI ATCGAT 2 cut(s) 557, 1406
BspEI TCCGGA 1 cut(s) 1289
BspFNI CGCG 1 cut(s) 1808
BspHI TCATGA 1 cut(s) 1719
BspLI GGNNCC 4 cut(s) 176, 302, 1452, 2153
BspMAI CTGCAG 1 cut(s) 761
BspPI GGATC 4 cut(s) 357, 1281, 1390, 1938
BspT104I TTCGAA 2 cut(s) 503, 598
BspT107I GGYRCC 1 cut(s) 2151
BspTNI GGTCTC 2 cut(s) 238, 514
BsrDI GCAATG 1 cut(s) 575
BsrFI RCCGGY 2 cut(s) 421, 603
BsrGI TGTACA 1 cut(s) 2093
BsrI ACTGG 3 cut(s) 1545, 1833, 2036
BssAI RCCGGY 2 cut(s) 421, 603
BssECI CCNNGG 7 cut(s) 206, 207, 888, 1038, 1098, 1413, 1512
BssMI GATC 8 cut(s) 238, 362, 526, 849, 897, 1286, 1382, 1930
BssNAI GTATAC 1 cut(s) 1989
BssT1I CCWWGG 3 cut(s) 888, 1413, 1512
Bst1107I GTATAC 1 cut(s) 1989
Bst2UI CCWGG 3 cut(s) 208, 1099, 1703
Bst4CI ACNGT 5 cut(s) 71, 1039, 1107, 1789, 2102
Bst6I CTCTTC 2 cut(s) 981, 1078
BstAPI GCANNNNNTGC 1 cut(s) 1870
BstAUI TGTACA 1 cut(s) 2093
BstBAI YACGTR 1 cut(s) 1649
BstBI TTCGAA 2 cut(s) 503, 598
BstC8I GCNNGC 5 cut(s) 423, 885, 1076, 1526, 2142
BstDEI CTNAG 6 cut(s) 320, 641, 650, 1812, 1846, 1953
BstDSI CCRYGG 1 cut(s) 1038
BstEII GGTNACC 2 cut(s) 1502, 1882
BstF5I GGATG 4 cut(s) 481, 1204, 1279, 1621
BstFNI CGCG 1 cut(s) 1808
BstHHI GCGC 3 cut(s) 14, 427, 1897
BstKTI GATC 8 cut(s) 241, 365, 529, 852, 900, 1289, 1385, 1933
BstMAI GTCTC 5 cut(s) 238, 514, 578, 1287, 1770
BstMBI GATC 8 cut(s) 238, 362, 526, 849, 897, 1286, 1382, 1930
BstMCI CGRYCG 1 cut(s) 241
BstMWI GCNNNNNNNGC 6 cut(s) 26, 35, 41, 1808, 1861, 1870
BstNI CCWGG 3 cut(s) 208, 1099, 1703
BstNSI RCATGY 2 cut(s) 1528, 2144
BstPI GGTNACC 2 cut(s) 1502, 1882
BstSCI CCNGG 5 cut(s) 171, 206, 349, 1097, 1701
BstSFI CTRYAG 1 cut(s) 757
BstSLI GKGCMC 1 cut(s) 2107
BstUI CGCG 1 cut(s) 1808
BstV2I GAAGAC 2 cut(s) 102, 1825
BstX2I RGATCY 3 cut(s) 362, 1286, 1382
BstYI RGATCY 3 cut(s) 362, 1286, 1382
BstZ17I GTATAC 1 cut(s) 1989
Bsu15I ATCGAT 2 cut(s) 557, 1406
BsuI GTATCC 1 cut(s) 639
BsuRI GGCC 7 cut(s) 345, 421, 887, 1138, 1412, 1713, 1908
BsuTUI ATCGAT 2 cut(s) 557, 1406
BtgI CCRYGG 1 cut(s) 1038
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 4 cut(s) 481, 1204, 1279, 1621
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 39, 2107
Cac8I GCNNGC 5 cut(s) 423, 885, 1076, 1526, 2142
CciI TCATGA 1 cut(s) 1719
CfoI GCGC 3 cut(s) 14, 427, 1897
Cfr10I RCCGGY 2 cut(s) 421, 603
Cfr13I GGNCC 4 cut(s) 175, 300, 343, 1456
ClaI ATCGAT 2 cut(s) 557, 1406
CpoI CGGWCCG 1 cut(s) 1456
CseI GACGC 1 cut(s) 1504
Csp6I GTAC 7 cut(s) 62, 83, 1468, 1646, 1840, 2094, 2152
CspAI ACCGGT 1 cut(s) 603
CspI CGGWCCG 1 cut(s) 1456
CviAII CATG 5 cut(s) 464, 1525, 1720, 1799, 2141
CviQI GTAC 7 cut(s) 62, 83, 1468, 1646, 1840, 2094, 2152
DdeI CTNAG 6 cut(s) 320, 641, 650, 1812, 1846, 1953
DpnI GATC 8 cut(s) 240, 364, 528, 851, 899, 1288, 1384, 1932
DpnII GATC 8 cut(s) 238, 362, 526, 849, 897, 1286, 1382, 1930
DraI TTTAAA 1 cut(s) 358
DraIII CACNNNGTG 2 cut(s) 667, 2102
DrdI GACNNNNNNGTC 1 cut(s) 2075
DriI GACNNNNNGTC 1 cut(s) 793
DseDI GACNNNNNNGTC 1 cut(s) 2075
EaeI YGGCCR 2 cut(s) 1410, 1711
Eam1104I CTCTTC 2 cut(s) 981, 1078
Eam1105I GACNNNNNGTC 1 cut(s) 793
EarI CTCTTC 2 cut(s) 981, 1078
EciI GGCGGA 1 cut(s) 46
Eco130I CCWWGG 3 cut(s) 888, 1413, 1512
Eco31I GGTCTC 2 cut(s) 238, 514
Eco47I GGWCC 3 cut(s) 175, 300, 1456
Eco91I GGTNACC 2 cut(s) 1502, 1882
EcoO109I RGGNCCY 2 cut(s) 175, 300
EcoO65I GGTNACC 2 cut(s) 1502, 1882
EcoRII CCWGG 3 cut(s) 206, 1097, 1701
EcoT14I CCWWGG 3 cut(s) 888, 1413, 1512
EcoT22I ATGCAT 1 cut(s) 465
ErhI CCWWGG 3 cut(s) 888, 1413, 1512
FaeI CATG 5 cut(s) 467, 1528, 1723, 1802, 2144
FalI AAGNNNNNCTT 2 cut(s) 966, 998
FaqI GGGAC 1 cut(s) 188
FatI CATG 5 cut(s) 463, 1524, 1719, 1798, 2140
FauI CCCGC 2 cut(s) 1071, 1387
FbaI TGATCA 2 cut(s) 849, 897
FblI GTMKAC 2 cut(s) 261, 1988
Fnu4HI GCNGC 2 cut(s) 36, 1809
FokI GGATG 4 cut(s) 488, 1211, 1286, 1628
Fsp4HI GCNGC 2 cut(s) 36, 1809
FspBI CTAG 5 cut(s) 672, 1683, 1823, 2120, 2176
GlaI GCGC 3 cut(s) 13, 426, 1896
GluI GCNGC 2 cut(s) 36, 1809
GsaI CCCAGC 1 cut(s) 711
GsuI CTGGAG 2 cut(s) 899, 1724
HaeIII GGCC 7 cut(s) 345, 421, 887, 1138, 1412, 1713, 1908
HapII CCGG 7 cut(s) 172, 350, 422, 604, 1139, 1290, 1454
HgaI GACGC 1 cut(s) 1504
HhaI GCGC 3 cut(s) 14, 427, 1897
Hin1II CATG 5 cut(s) 467, 1528, 1723, 1802, 2144
Hin6I GCGC 3 cut(s) 12, 425, 1895
HinP1I GCGC 3 cut(s) 12, 425, 1895
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 2 cut(s) 27, 518
HinfI GANTC 9 cut(s) 282, 312, 385, 394, 749, 1030, 1068, 1795, 2087
HpaII CCGG 7 cut(s) 172, 350, 422, 604, 1139, 1290, 1454
HphI GGTGA 7 cut(s) 457, 1246, 1499, 1514, 1691, 1786, 1876
Hpy166II GTNNAC 8 cut(s) 64, 262, 688, 1569, 1950, 1989, 2096, 2105
Hpy188I TCNGA 7 cut(s) 238, 321, 332, 367, 799, 1208, 1849
Hpy8I GTNNAC 8 cut(s) 64, 262, 688, 1569, 1950, 1989, 2096, 2105
Hpy99I CGWCG 2 cut(s) 293, 724
HpyAV CCTTC 6 cut(s) 499, 758, 1518, 1717, 1779, 2029
HpyCH4III ACNGT 5 cut(s) 71, 1039, 1107, 1789, 2102
HpyCH4IV ACGT 4 cut(s) 618, 684, 836, 1648
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 35, 41, 1808, 1861, 1870
HpyF3I CTNAG 6 cut(s) 320, 641, 650, 1812, 1846, 1953
HpySE526I ACGT 4 cut(s) 618, 684, 836, 1648
Hsp92II CATG 5 cut(s) 467, 1528, 1723, 1802, 2144
HspAI GCGC 3 cut(s) 12, 425, 1895
Kpn2I TCCGGA 1 cut(s) 1289
KpnI GGTACC 1 cut(s) 2155
KroI GCCGGC 1 cut(s) 421
KroNI GCCGGC 1 cut(s) 423
Ksp22I TGATCA 2 cut(s) 849, 897
Kzo9I GATC 8 cut(s) 238, 362, 526, 849, 897, 1286, 1382, 1930
LmnI GCTCC 6 cut(s) 128, 874, 880, 1025, 1450, 1981
LweI GCATC 9 cut(s) 549, 792, 1084, 1189, 1695, 1737, 1842, 2052, 2125
MaeI CTAG 5 cut(s) 672, 1683, 1823, 2120, 2176
MaeII ACGT 4 cut(s) 618, 684, 836, 1648
MaeIII GTNAC 7 cut(s) 667, 727, 832, 1087, 1252, 1502, 1882
MalI GATC 8 cut(s) 240, 364, 528, 851, 899, 1288, 1384, 1932
MboI GATC 8 cut(s) 238, 362, 526, 849, 897, 1286, 1382, 1930
MboII GAAGA 7 cut(s) 85, 102, 634, 998, 1095, 1544, 1830
MflI RGATCY 3 cut(s) 362, 1286, 1382
MhlI GDGCHC 1 cut(s) 2107
MlsI TGGCCA 2 cut(s) 1412, 1713
MluCI AATT 9 cut(s) 129, 409, 1017, 1051, 1490, 1601, 1609, 2161, 2166
MluNI TGGCCA 2 cut(s) 1412, 1713
MlyI GAGTC 2 cut(s) 291, 1062
MmeI TCCRAC 1 cut(s) 726
Mox20I TGGCCA 2 cut(s) 1412, 1713
Mph1103I ATGCAT 1 cut(s) 465
MroI TCCGGA 1 cut(s) 1289
MroNI GCCGGC 1 cut(s) 421
MscI TGGCCA 2 cut(s) 1412, 1713
MseI TTAA 9 cut(s) 357, 486, 678, 714, 1058, 1121, 1317, 2112, 2165
MslI CAYNNNNRTG 1 cut(s) 1268
Msp20I TGGCCA 2 cut(s) 1412, 1713
MspI CCGG 7 cut(s) 172, 350, 422, 604, 1139, 1290, 1454
MspR9I CCNGG 5 cut(s) 173, 208, 351, 1099, 1703
MvaI CCWGG 3 cut(s) 208, 1099, 1703
MvnI CGCG 1 cut(s) 1808
MwoI GCNNNNNNNGC 6 cut(s) 26, 35, 41, 1808, 1861, 1870
NaeI GCCGGC 1 cut(s) 423
NciI CCSGG 2 cut(s) 173, 351
NdeII GATC 8 cut(s) 238, 362, 526, 849, 897, 1286, 1382, 1930
NgoMIV GCCGGC 1 cut(s) 421
NlaIII CATG 5 cut(s) 467, 1528, 1723, 1802, 2144
NlaIV GGNNCC 4 cut(s) 176, 302, 1452, 2153
NmuCI GTSAC 5 cut(s) 667, 1087, 1252, 1502, 1882
NsiI ATGCAT 1 cut(s) 465
NspI RCATGY 2 cut(s) 1528, 2144
NspV TTCGAA 2 cut(s) 503, 598
PaeI GCATGC 2 cut(s) 1528, 2144
PagI TCATGA 1 cut(s) 1719
PasI CCCWGGG 1 cut(s) 207
PcsI WCGNNNNNNNCGW 1 cut(s) 509
PdiI GCCGGC 1 cut(s) 423
PfeI GAWTC 7 cut(s) 312, 385, 394, 749, 1030, 1795, 2087
PinAI ACCGGT 1 cut(s) 603
PkrI GCNGC 2 cut(s) 37, 1810
Ple19I CGATCG 1 cut(s) 241
PleI GAGTC 2 cut(s) 290, 1062
PpsI GAGTC 2 cut(s) 290, 1062
Ppu21I YACGTR 1 cut(s) 1649
PpuMI RGGWCCY 2 cut(s) 175, 300
PshBI ATTAAT 1 cut(s) 486
PsiI TTATAA 2 cut(s) 945, 1050
Psp5II RGGWCCY 2 cut(s) 175, 300
Psp6I CCWGG 3 cut(s) 206, 1097, 1701
PspEI GGTNACC 2 cut(s) 1502, 1882
PspFI CCCAGC 1 cut(s) 707
PspGI CCWGG 3 cut(s) 206, 1097, 1701
PspN4I GGNNCC 4 cut(s) 176, 302, 1452, 2153
PspPI GGNCC 4 cut(s) 175, 300, 343, 1456
PspPPI RGGWCCY 2 cut(s) 175, 300
PstI CTGCAG 1 cut(s) 761
PsuI RGATCY 3 cut(s) 362, 1286, 1382
PvuI CGATCG 1 cut(s) 241
RsaI GTAC 7 cut(s) 63, 84, 1469, 1647, 1841, 2095, 2153
RsaNI GTAC 7 cut(s) 62, 83, 1468, 1646, 1840, 2094, 2152
RseI CAYNNNNRTG 1 cut(s) 1268
Rsr2I CGGWCCG 1 cut(s) 1456
RsrII CGGWCCG 1 cut(s) 1456
SalI GTCGAC 1 cut(s) 260
SaqAI TTAA 9 cut(s) 357, 486, 678, 714, 1058, 1121, 1317, 2112, 2165
SatI GCNGC 2 cut(s) 36, 1809
Sau3AI GATC 8 cut(s) 238, 362, 526, 849, 897, 1286, 1382, 1930
Sau96I GGNCC 4 cut(s) 175, 300, 343, 1456
SchI GAGTC 2 cut(s) 291, 1062
ScrFI CCNGG 5 cut(s) 173, 208, 351, 1099, 1703
SduI GDGCHC 1 cut(s) 2107
SfaNI GCATC 9 cut(s) 549, 792, 1084, 1189, 1695, 1737, 1842, 2052, 2125
SfcI CTRYAG 1 cut(s) 757
SfuI TTCGAA 2 cut(s) 503, 598
SinI GGWCC 3 cut(s) 175, 300, 1456
SmiMI CAYNNNNRTG 1 cut(s) 1268
SmlI CTYRAG 5 cut(s) 77, 389, 563, 1001, 2156
SmoI CTYRAG 5 cut(s) 77, 389, 563, 1001, 2156
SphI GCATGC 2 cut(s) 1528, 2144
Sse9I AATT 9 cut(s) 129, 409, 1017, 1051, 1490, 1601, 1609, 2161, 2166
SsiI CCGC 5 cut(s) 36, 57, 1078, 1394, 1808
SspI AATATT 1 cut(s) 1579
SspMI CTAG 5 cut(s) 672, 1683, 1823, 2120, 2176
StyD4I CCNGG 5 cut(s) 171, 206, 349, 1097, 1701
StyI CCWWGG 3 cut(s) 888, 1413, 1512
TaaI ACNGT 5 cut(s) 71, 1039, 1107, 1789, 2102
TaiI ACGT 4 cut(s) 621, 687, 839, 1651
TaqII GACCGA 1 cut(s) 1473
TasI AATT 9 cut(s) 129, 409, 1017, 1051, 1490, 1601, 1609, 2161, 2166
TatI WGTACW 2 cut(s) 1839, 2093
TauI GCSGC 2 cut(s) 38, 1811
TfiI GAWTC 7 cut(s) 312, 385, 394, 749, 1030, 1795, 2087
Tru1I TTAA 9 cut(s) 357, 486, 678, 714, 1058, 1121, 1317, 2112, 2165
Tru9I TTAA 9 cut(s) 357, 486, 678, 714, 1058, 1121, 1317, 2112, 2165
TscAI CASTG 2 cut(s) 46, 2107
TseFI GTSAC 5 cut(s) 667, 1087, 1252, 1502, 1882
Tsp45I GTSAC 5 cut(s) 667, 1087, 1252, 1502, 1882
TspDTI ATGAA 6 cut(s) 741, 926, 1140, 1632, 1736, 1787
TspRI CASTG 2 cut(s) 46, 2107
VneI GTGCAC 1 cut(s) 2103
VpaK11BI GGWCC 3 cut(s) 175, 300, 1456
VspI ATTAAT 1 cut(s) 486
XapI RAATTY 1 cut(s) 2161
XbaI TCTAGA 1 cut(s) 2119
XceI RCATGY 2 cut(s) 1528, 2144
XmiI GTMKAC 2 cut(s) 261, 1988
XspI CTAG 5 cut(s) 672, 1683, 1823, 2120, 2176
Zsp2I ATGCAT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.