RchiOBHm_Chr4g0434041

Pentatricopeptide repeat-containing protein At1g22960

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011481
Physical Location & Seq
Forward (+)
57940938 .. 57944227
3290 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40251

Sequence Viewer

Length: 2145 bp
ATGACCCTCTGCGCCAGAGCTTCTAAAGCTTTCAAGGTACGCCTCCCTCCTCTACCCCTCTCCTCCTCCTTCCACTCCCCTTCCCTCCCCGCCACGTCACCTCTCTCCGACACCCACTACCGCGACCTCATCTTCTCCACCATCCGAACAACCCCCTCAGCTTTCCGCACCCGCGACTGGTCCTCCCACCGCTTCCGCCCCGTGATTTCCGACCCGGACCTCTTCGTCCGCGTCCTCGCCCAGATTCGGGCCAGGCCCACCGTCGCCCTCCGCTTCTTCCGATGGGCCGAGGCCCAGCCGGAGGTTAAGCGGTCCGAGTTTGTCTTCTGTGTCATTCTCGAGATTCTGGCCCACCACAACATGATGAGGTCTGCGTATTGGGTGATGGAGAGAGTGATTAGTGTAGACATGCATCATGGCATTGTGGATGTTTTGATTGATGAGTATGTTTTCGCAAAGGTCTCCATCAAGCTGCTTGATCTCTTGTTGTGGGTGTGCGCCAACAAGTTGTTGGTGGAGCAATGCCTGTCCATTTTCGATAAGATGATAACCAATGGGTTGTTGCCCCATGTGAATAACTGTAATAGGCTTCTTAGGATGCTTAGGGATAGGAACCTTGCCAGTAGAGCTCAAGAGGTTTATAGGAGGATGGGTGAGGCCGGCATCAAGCCCACCATTGTTACTTACAATACCATGCTGGATTCGTTTTGCAAGCAAGGGGAAGTGCAGCAGGCGCTTCACCTTGTCTCGGAGATGCAGAAGGTGGAGTGCTTCCCCAATGACGTGACCTACAATGTGTTGATCAATGGCTTGTCGAAGAAAGGGAAACTGGAGCAGGCCGAGGGGCTGTTGCAAGATATGTTGAAGTCGGGATTGAGGGCTTCAGCGTACACGTATAACCCTTTGATTTGTGGGTATTGCAAGAGGGGACTGCTTGCGGAGGCATTGAATCTCGGGGAAGAGATGGCGGCAAGAGGAGCCGCTCCTACTGTGGCTACTTATAACTCGATAATGTATGGCCTCTGCAAGTGGGGCAGAGTTACTGAGGCCAGGGACTGGTTTACTAATATGTTAAAGAAACGATTGTCGCCTGATATAGTTTCATACAACACTCTTATATATGGGTATTGTAGGTTAGGGGACATATGGAATGCTTTTCTACTGTTTGATGAGTTGAGACGGAGGGCTCTCACTCCTACTGTCATTACTTATAATACACTCATGGATGGTCTATGCAGATTGGGGGAGTTCGAGGTTGCTCAGCAGCTTAAAAAAGAAATGGTCAATCAAGGAATTTTCCCTGATGTTTTTACATATACTATTCTTGTAAATGGATATTGCAAGGCAGGAAATTTATCAAAGGCCAGGGAGTTATTTGATGAGATGTTGCATAACGGACTGGAGCCGGATCGCTTTGCATACAATACTCGAATGGTGGGGGAATTGGATCTTGGTGACCCATCCAAGGCATTTAATATGCAAGAAGAAATGCTAGCAAAGGGTTTGCCTCCTGATTTGTTCACATATAATGTTTTTGTGGATGGAGTATGTAAATTGGGCAACTTGAGTGAAGCATATAACATGTTGCAGAAAATGGTACATGATGGTCTCGTGCCAGATCATGTAACATACACTAGCATCATTCATGCTTACCTGGTAAGTGGCCGCCTCAGGAAGGCTAGAGAGGTATATGATGAGATGCTGAGCAAAGACGTGCATCCCTCAGTTGTAACTTACACAGTACTGATTCATGCTCATGCATCTAAGGGGAGACTGGAACTAGCATATATGTACTTCTCAGAGATGCAAGAGAAGCGTGTTCTGCCAAATGTTGTTACCTACAATGCTCTGATTAATGGTCTTTGTAAAGTTATGAGAATGGACCAGGCTTATGAGTTCTTTACTCATATGGAGGAAAAGGGTATACTTCCAAATAAGTATACATACACTATTCTAATAAATGAGAACTGCAACATCGGCAATTGGAAGGAGGCTTTGAGATTGTACAAACAGATGCTTGACAGAGAGATTGAGCCTGACTTTTGTACGCACAGTGCACTTTTTAAGCAACTAGACAGAGACTATAAACTGCATGCGGTTCATTGCCTTGAGAGTTTAATTCTTGCCACAGAAGAATCTAGCTAG

Protein Analysis

714

Amino Acids

81.84

Weight (kDa)

8.15

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 161 - 275 9.9e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 177 - 236 1.1e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 190 - 238 8.9e-08 PPR repeat family
TPR_24 PF23276 206 - 290 2.1e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 221 - 253 2.7e-11 PPR repeat
PPR_2 PF13041 224 - 273 1.5e-18 PPR repeat family
PPR_3 PF13812 226 - 269 1.3e-07 Pentatricopeptide repeat domain
PPR PF01535 227 - 254 1.6e-06 PPR repeat
PPR_1 PF12854 257 - 287 3.7e-09 PPR repeat
PPR_2 PF13041 259 - 303 1.1e-12 PPR repeat family
PPR PF01535 262 - 292 3.1e-08 PPR repeat
PPR_3 PF13812 283 - 338 2e-07 Pentatricopeptide repeat domain
TPR_24 PF23276 286 - 391 1.1e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 291 - 322 4e-08 PPR repeat
PPR_2 PF13041 296 - 343 3.9e-13 PPR repeat family
PPR_2 PF13041 329 - 378 2.1e-14 PPR repeat family
PPR_3 PF13812 355 - 409 3.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 361 - 392 1.3e-10 PPR repeat
PPR_2 PF13041 364 - 413 1.1e-16 PPR repeat family
PPR PF01535 367 - 395 1.2e-06 PPR repeat
PPR_1 PF12854 397 - 428 1.3e-09 PPR repeat
PPR_2 PF13041 399 - 448 1.3e-18 PPR repeat family
PPR PF01535 402 - 432 3e-06 PPR repeat
PPR_3 PF13812 423 - 476 1.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 431 - 462 2.5e-15 PPR repeat
PPR PF01535 437 - 467 9.7e-10 PPR repeat
PPR_2 PF13041 442 - 476 1.1e-08 PPR repeat family
PPR_2 PF13041 469 - 518 1.3e-09 PPR repeat family
PPR_1 PF12854 500 - 533 9.7e-10 PPR repeat
PPR_2 PF13041 507 - 551 1.5e-10 PPR repeat family
PPR_3 PF13812 528 - 579 6.7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 535 - 567 1.2e-06 PPR repeat
PPR_2 PF13041 539 - 587 1.2e-14 PPR repeat family
PPR PF01535 542 - 571 6.9e-07 PPR repeat
PPR_long PF17177 556 - 667 6e-12 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 564 - 619 4.6e-14 Pentatricopeptide repeat domain
PPR_1 PF12854 570 - 603 1e-05 PPR repeat
PPR_2 PF13041 574 - 623 1.1e-17 PPR repeat family
PPR PF01535 577 - 606 3.4e-06 PPR repeat
PPR_1 PF12854 606 - 638 2.1e-12 PPR repeat
PPR_2 PF13041 609 - 658 1.7e-19 PPR repeat family
PPR PF01535 612 - 642 2.9e-07 PPR repeat
PPR_3 PF13812 635 - 689 1.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 640 - 672 1.5e-07 PPR repeat
PPR_2 PF13041 653 - 691 2.1e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22960 AT1G22960
fragaria_vesca FvH4_4g26410
malus_domestica MD13G1032800.v1.1 MD16G1034800.v1.1
pyrus_communis pycom13g02830 pycom16g02960
rosa_chinensis RchiOBHm_Chr4g0434041
rosa_laevigata RLG00000006706
rosa_multiflora Rmu_co8498505.1_g000001 Rmu_sc0000352.1_g000026
rosa_roxburghii Rroxscaffold_5G00375450
rosa_rugosa Rorug04G0272900
rosa_samantha Rh4AG328600 Rh4BG335900 Rh4CG351400 Rh4DG332300 Rh5AG231100 Rh5BG086200
rosa_wichuraiana Rw4G028490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1002, 1212
AasI GACNNNNNNGTC 1 cut(s) 224
AccB7I CCANNNNNTGG 1 cut(s) 1056
AccBSI CCGCTC 1 cut(s) 983
AccI GTMKAC 3 cut(s) 405, 1924, 1940
AccII CGCG 3 cut(s) 123, 174, 231
AclWI GGATC 2 cut(s) 1416, 1455
AcoI YGGCCR 1 cut(s) 1663
AcsI RAATTY 2 cut(s) 1293, 1351
AcuI CTGAAG 1 cut(s) 867
AfaI GTAC 7 cut(s) 39, 890, 1599, 1743, 1793, 2006, 2047
AfiI CCNNNNNNNGG 8 cut(s) 177, 246, 247, 301, 748, 1056, 1465, 1675
AflIII ACRYGT 2 cut(s) 891, 1581
AgsI TTSAA 3 cut(s) 34, 865, 949
AjiI CACGTC 3 cut(s) 96, 784, 1714
AjnI CCWGG 5 cut(s) 251, 1049, 1364, 1653, 1884
AjuI GAANNNNNNNTTGG 2 cut(s) 1434, 1466
AluBI AGCT 7 cut(s) 20, 29, 161, 472, 629, 1267, 2142
AluI AGCT 7 cut(s) 20, 29, 161, 472, 629, 1267, 2142
Alw21I GWGCWC 2 cut(s) 631, 2059
Alw26I GTCTC 6 cut(s) 466, 751, 1171, 1613, 1765, 2073
Alw44I GTGCAC 1 cut(s) 2055
AlwI GGATC 2 cut(s) 1416, 1455
AlwNI CAGNNNCTG 1 cut(s) 1056
Ama87I CYCGRG 2 cut(s) 338, 953
ApaLI GTGCAC 1 cut(s) 2055
ApeKI GCWGC 3 cut(s) 472, 727, 1264
ApoI RAATTY 2 cut(s) 1293, 1351
AseI ATTAAT 1 cut(s) 1854
AspLEI GCGC 3 cut(s) 14, 500, 736
AspS9I GGNCC 9 cut(s) 180, 217, 249, 255, 285, 292, 312, 349, 1882
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 5 cut(s) 90, 394, 665, 731, 1466
AsuNHI GCTAGC 1 cut(s) 1492
AvaI CYCGRG 2 cut(s) 338, 953
AvaII GGWCC 4 cut(s) 180, 217, 312, 1882
AxyI CCTNAGG 1 cut(s) 1670
BaeGI GKGCMC 1 cut(s) 2059
BanII GRGCYC 2 cut(s) 631, 1189
BauI CACGAG 1 cut(s) 1610
BbsI GAAGAC 1 cut(s) 316
Bbv12I GWGCWC 2 cut(s) 631, 2059
BbvCI CCTCAGC 1 cut(s) 157
BbvI GCAGC 3 cut(s) 459, 739, 1276
BciT130I CCWGG 5 cut(s) 253, 1051, 1366, 1655, 1886
BclI TGATCA 1 cut(s) 801
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 6 cut(s) 466, 751, 1171, 1613, 1765, 2073
BfaI CTAG 7 cut(s) 1493, 1635, 1680, 1781, 2072, 2139, 2143
BfoI RGCGCY 1 cut(s) 737
BisI GCNGC 6 cut(s) 473, 728, 969, 981, 1265, 1666
BlpI GCTNAGC 2 cut(s) 1260, 1703
BlsI GCNGC 6 cut(s) 474, 729, 970, 982, 1266, 1667
BmcAI AGTACT 1 cut(s) 1743
Bme1390I CCNGG 6 cut(s) 215, 253, 1051, 1366, 1655, 1886
Bme18I GGWCC 4 cut(s) 180, 217, 312, 1882
BmeT110I CYCGRG 2 cut(s) 338, 953
BmgBI CACGTC 3 cut(s) 96, 784, 1714
BmgT120I GGNCC 9 cut(s) 180, 217, 249, 255, 285, 292, 312, 349, 1882
BmiI GGNNCC 3 cut(s) 614, 979, 1404
BmrFI CCNGG 6 cut(s) 215, 253, 1051, 1366, 1655, 1886
BmtI GCTAGC 1 cut(s) 1496
BpiI GAAGAC 1 cut(s) 316
BpmI CTGGAG 2 cut(s) 851, 1421
Bpu10I CCTNAGC 2 cut(s) 157, 602
Bpu1102I GCTNAGC 2 cut(s) 1260, 1703
BpuEI CTTGAG 3 cut(s) 615, 1585, 2129
BpuMI CCSGG 1 cut(s) 215
BsaAI YACGTR 1 cut(s) 894
BsaI GGTCTC 2 cut(s) 466, 1613
BsaJI CCNNGG 5 cut(s) 288, 840, 1050, 1365, 1464
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 637, 667
Bsc4I CCNNNNNNNGG 8 cut(s) 177, 246, 247, 301, 748, 1056, 1465, 1675
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 6 cut(s) 182, 621, 834, 1061, 1404, 1779
Bse21I CCTNAGG 1 cut(s) 1670
Bse3DI GCAATG 2 cut(s) 527, 2101
BseBI CCWGG 5 cut(s) 253, 1051, 1366, 1655, 1886
BseDI CCNNGG 5 cut(s) 288, 840, 1050, 1365, 1464
BseGI GGATG 8 cut(s) 141, 433, 603, 654, 1231, 1460, 1546, 1717
BseLI CCNNNNNNNGG 8 cut(s) 177, 246, 247, 301, 748, 1056, 1465, 1675
BseMI GCAATG 2 cut(s) 527, 2101
BseMII CTCAG 7 cut(s) 171, 1035, 1274, 1684, 1694, 1737, 1812
BseNI ACTGG 6 cut(s) 182, 621, 834, 1061, 1404, 1779
BseRI GAGGAG 4 cut(s) 39, 52, 55, 990
BseSI GKGCMC 1 cut(s) 2059
BseXI GCAGC 3 cut(s) 459, 739, 1276
BseYI CCCAGC 1 cut(s) 294
BsgI GTGCAG 1 cut(s) 746
Bsh1236I CGCG 3 cut(s) 123, 174, 231
BsiHKAI GWGCWC 2 cut(s) 631, 2059
BsiHKCI CYCGRG 2 cut(s) 338, 953
BsiSI CCGG 4 cut(s) 215, 299, 660, 1406
BslFI GGGAC 3 cut(s) 942, 1067, 1154
BslI CCNNNNNNNGG 8 cut(s) 177, 246, 247, 301, 748, 1056, 1465, 1675
BsmAI GTCTC 6 cut(s) 466, 751, 1171, 1613, 1765, 2073
BsmBI CGTCTC 1 cut(s) 1171
BsmFI GGGAC 3 cut(s) 942, 1067, 1154
BsmI GAATGC 1 cut(s) 1156
Bso31I GGTCTC 2 cut(s) 466, 1613
BsoBI CYCGRG 2 cut(s) 338, 953
Bsp1286I GDGCHC 3 cut(s) 631, 1189, 2059
Bsp1407I TGTACA 1 cut(s) 2004
Bsp143I GATC 5 cut(s) 478, 801, 1408, 1447, 1618
Bsp1720I GCTNAGC 2 cut(s) 1260, 1703
BspCNI CTCAG 7 cut(s) 170, 1036, 1273, 1683, 1695, 1736, 1811
BspFNI CGCG 3 cut(s) 123, 174, 231
BspLI GGNNCC 3 cut(s) 614, 979, 1404
BspOI GCTAGC 1 cut(s) 1496
BspPI GGATC 2 cut(s) 1416, 1455
BspTNI GGTCTC 2 cut(s) 466, 1613
BsrBI CCGCTC 1 cut(s) 983
BsrDI GCAATG 2 cut(s) 527, 2101
BsrFI RCCGGY 1 cut(s) 659
BsrGI TGTACA 1 cut(s) 2004
BsrI ACTGG 6 cut(s) 182, 621, 834, 1061, 1404, 1779
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 5 cut(s) 288, 840, 1050, 1365, 1464
BssMI GATC 5 cut(s) 478, 801, 1408, 1447, 1618
BssNAI GTATAC 2 cut(s) 1925, 1941
BssSI CACGAG 1 cut(s) 1610
BssT1I CCWWGG 1 cut(s) 1464
Bst1107I GTATAC 2 cut(s) 1925, 1941
Bst2BI CACGAG 1 cut(s) 1610
Bst2UI CCWGG 5 cut(s) 253, 1051, 1366, 1655, 1886
Bst4CI ACNGT 7 cut(s) 262, 581, 991, 1164, 1201, 1741, 2054
Bst6I CTCTTC 2 cut(s) 227, 954
BstAUI TGTACA 1 cut(s) 2004
BstBAI YACGTR 1 cut(s) 894
BstC8I GCNNGC 7 cut(s) 661, 713, 732, 837, 936, 1494, 2094
BstEII GGTNACC 1 cut(s) 1454
BstENI CCTNNNNNAGG 1 cut(s) 1673
BstF5I GGATG 8 cut(s) 141, 433, 603, 654, 1231, 1460, 1546, 1717
BstFNI CGCG 3 cut(s) 123, 174, 231
BstH2I RGCGCY 1 cut(s) 737
BstHHI GCGC 3 cut(s) 14, 500, 736
BstKTI GATC 5 cut(s) 481, 804, 1411, 1450, 1621
BstMAI GTCTC 6 cut(s) 466, 751, 1171, 1613, 1765, 2073
BstMBI GATC 5 cut(s) 478, 801, 1408, 1447, 1618
BstMWI GCNNNNNNNGC 8 cut(s) 26, 626, 733, 977, 1032, 1813, 1822, 1977
BstNI CCWGG 5 cut(s) 253, 1051, 1366, 1655, 1886
BstNSI RCATGY 3 cut(s) 412, 1585, 2096
BstPI GGTNACC 1 cut(s) 1454
BstSCI CCNGG 6 cut(s) 213, 251, 1049, 1364, 1653, 1884
BstSLI GKGCMC 1 cut(s) 2059
BstUI CGCG 3 cut(s) 123, 174, 231
BstV1I GCAGC 3 cut(s) 459, 739, 1276
BstV2I GAAGAC 1 cut(s) 316
BstX2I RGATCY 1 cut(s) 1447
BstYI RGATCY 1 cut(s) 1447
BstZ17I GTATAC 2 cut(s) 1925, 1941
Bsu36I CCTNAGG 1 cut(s) 1670
BtrI CACGTC 3 cut(s) 96, 784, 1714
BtsCI GGATG 8 cut(s) 141, 433, 603, 654, 1231, 1460, 1546, 1717
BtsIMutI CAGTG 1 cut(s) 2059
Cac8I GCNNGC 7 cut(s) 661, 713, 732, 837, 936, 1494, 2094
CaiI CAGNNNCTG 1 cut(s) 1056
CfoI GCGC 3 cut(s) 14, 500, 736
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 9 cut(s) 180, 217, 249, 255, 285, 292, 312, 349, 1882
CpoI CGGWCCG 1 cut(s) 312
CseI GACGC 1 cut(s) 220
CsiI ACCWGGT 1 cut(s) 1653
Csp6I GTAC 7 cut(s) 38, 889, 1598, 1742, 1792, 2005, 2046
CspI CGGWCCG 1 cut(s) 312
CviQI GTAC 7 cut(s) 38, 889, 1598, 1742, 1792, 2005, 2046
DpnI GATC 5 cut(s) 480, 803, 1410, 1449, 1620
DpnII GATC 5 cut(s) 478, 801, 1408, 1447, 1618
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 1663
Eam1104I CTCTTC 2 cut(s) 227, 954
EarI CTCTTC 2 cut(s) 227, 954
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 1 cut(s) 1464
Eco24I GRGCYC 2 cut(s) 631, 1189
Eco31I GGTCTC 2 cut(s) 466, 1613
Eco47I GGWCC 4 cut(s) 180, 217, 312, 1882
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 1 cut(s) 867
Eco81I CCTNAGG 1 cut(s) 1670
Eco88I CYCGRG 2 cut(s) 338, 953
Eco91I GGTNACC 1 cut(s) 1454
EcoICRI GAGCTC 1 cut(s) 629
EcoNI CCTNNNNNAGG 1 cut(s) 1673
EcoO65I GGTNACC 1 cut(s) 1454
EcoRII CCWGG 5 cut(s) 251, 1049, 1364, 1653, 1884
EcoT14I CCWWGG 1 cut(s) 1464
EcoT22I ATGCAT 2 cut(s) 414, 1762
EcoT38I GRGCYC 2 cut(s) 631, 1189
ErhI CCWWGG 1 cut(s) 1464
Esp3I CGTCTC 1 cut(s) 1171
FaqI GGGAC 3 cut(s) 942, 1067, 1154
FauI CCCGC 2 cut(s) 97, 179
FauNDI CATATG 2 cut(s) 1145, 1908
FbaI TGATCA 1 cut(s) 801
FblI GTMKAC 3 cut(s) 405, 1924, 1940
Fnu4HI GCNGC 6 cut(s) 473, 728, 969, 981, 1265, 1666
FokI GGATG 8 cut(s) 128, 440, 610, 661, 1238, 1447, 1553, 1704
FriOI GRGCYC 2 cut(s) 631, 1189
Fsp4HI GCNGC 6 cut(s) 473, 728, 969, 981, 1265, 1666
FspBI CTAG 7 cut(s) 1493, 1635, 1680, 1781, 2072, 2139, 2143
GlaI GCGC 3 cut(s) 13, 499, 735
GluI GCNGC 6 cut(s) 473, 728, 969, 981, 1265, 1666
GsaI CCCAGC 1 cut(s) 298
GsuI CTGGAG 2 cut(s) 851, 1421
HaeII RGCGCY 1 cut(s) 737
HapII CCGG 4 cut(s) 215, 299, 660, 1406
HgaI GACGC 1 cut(s) 220
HhaI GCGC 3 cut(s) 14, 500, 736
Hin6I GCGC 3 cut(s) 12, 498, 734
HinP1I GCGC 3 cut(s) 12, 498, 734
HindIII AAGCTT 1 cut(s) 27
HinfI GANTC 6 cut(s) 244, 343, 701, 949, 1747, 2135
HpaII CCGG 4 cut(s) 215, 299, 660, 1406
HphI GGTGA 5 cut(s) 90, 394, 665, 731, 1466
Hpy166II GTNNAC 7 cut(s) 406, 891, 1062, 1521, 1925, 1941, 2057
Hpy188I TCNGA 8 cut(s) 109, 146, 211, 281, 316, 751, 1801, 1851
Hpy188III TCNNGA 6 cut(s) 338, 340, 632, 870, 1511, 1672
Hpy8I GTNNAC 7 cut(s) 406, 891, 1062, 1521, 1925, 1941, 2057
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 5 cut(s) 79, 90, 754, 1669, 1981
HpyCH4III ACNGT 7 cut(s) 262, 581, 991, 1164, 1201, 1741, 2054
HpyCH4IV ACGT 4 cut(s) 95, 783, 893, 1713
HpyF10VI GCNNNNNNNGC 8 cut(s) 26, 626, 733, 977, 1032, 1813, 1822, 1977
HpySE526I ACGT 4 cut(s) 95, 783, 893, 1713
HspAI GCGC 3 cut(s) 12, 498, 734
KroI GCCGGC 1 cut(s) 659
KroNI GCCGGC 1 cut(s) 661
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 5 cut(s) 478, 801, 1408, 1447, 1618
LmnI GCTCC 5 cut(s) 517, 832, 977, 988, 1402
Lsp1109I GCAGC 3 cut(s) 459, 739, 1276
MabI ACCWGGT 1 cut(s) 1653
MaeI CTAG 7 cut(s) 1493, 1635, 1680, 1781, 2072, 2139, 2143
MaeII ACGT 4 cut(s) 95, 783, 893, 1713
MaeIII GTNAC 8 cut(s) 96, 679, 784, 1039, 1454, 1624, 1729, 1834
MalI GATC 5 cut(s) 480, 803, 1410, 1449, 1620
MbiI CCGCTC 1 cut(s) 983
MboI GATC 5 cut(s) 478, 801, 1408, 1447, 1618
MboII GAAGA 8 cut(s) 124, 214, 268, 316, 829, 971, 1496, 2144
MfeI CAATTG 1 cut(s) 1981
MflI RGATCY 1 cut(s) 1447
MhlI GDGCHC 3 cut(s) 631, 1189, 2059
MluCI AATT 6 cut(s) 1293, 1351, 1442, 1553, 1981, 2118
MmeI TCCRAC 2 cut(s) 132, 234
Mph1103I ATGCAT 2 cut(s) 414, 1762
MroNI GCCGGC 1 cut(s) 659
MseI TTAA 7 cut(s) 306, 1073, 1269, 1473, 1854, 2064, 2117
MslI CAYNNNNRTG 1 cut(s) 1755
MspI CCGG 4 cut(s) 215, 299, 660, 1406
MspR9I CCNGG 6 cut(s) 215, 253, 1051, 1366, 1655, 1886
MunI CAATTG 1 cut(s) 1981
Mva1269I GAATGC 1 cut(s) 1156
MvaI CCWGG 5 cut(s) 253, 1051, 1366, 1655, 1886
MvnI CGCG 3 cut(s) 123, 174, 231
MwoI GCNNNNNNNGC 8 cut(s) 26, 626, 733, 977, 1032, 1813, 1822, 1977
NaeI GCCGGC 1 cut(s) 661
NciI CCSGG 1 cut(s) 215
NdeI CATATG 2 cut(s) 1145, 1908
NdeII GATC 5 cut(s) 478, 801, 1408, 1447, 1618
NgoMIV GCCGGC 1 cut(s) 659
NheI GCTAGC 1 cut(s) 1492
NlaIV GGNNCC 3 cut(s) 614, 979, 1404
NmeAIII GCCGAG 2 cut(s) 313, 865
NmuCI GTSAC 3 cut(s) 96, 784, 1454
NsiI ATGCAT 2 cut(s) 414, 1762
NspI RCATGY 3 cut(s) 412, 1585, 2096
PaeI GCATGC 1 cut(s) 2096
PaeR7I CTCGAG 1 cut(s) 338
PciI ACATGT 1 cut(s) 1581
PctI GAATGC 1 cut(s) 1156
PdiI GCCGGC 1 cut(s) 661
PfeI GAWTC 6 cut(s) 244, 343, 701, 949, 1747, 2135
PflMI CCANNNNNTGG 1 cut(s) 1056
PkrI GCNGC 6 cut(s) 474, 729, 970, 982, 1266, 1667
Ppu21I YACGTR 1 cut(s) 894
PscI ACATGT 1 cut(s) 1581
PshBI ATTAAT 1 cut(s) 1854
PsiI TTATAA 2 cut(s) 1002, 1212
Psp124BI GAGCTC 1 cut(s) 631
Psp6I CCWGG 5 cut(s) 251, 1049, 1364, 1653, 1884
PspEI GGTNACC 1 cut(s) 1454
PspFI CCCAGC 1 cut(s) 294
PspGI CCWGG 5 cut(s) 251, 1049, 1364, 1653, 1884
PspN4I GGNNCC 3 cut(s) 614, 979, 1404
PspPI GGNCC 9 cut(s) 180, 217, 249, 255, 285, 292, 312, 349, 1882
PstNI CAGNNNCTG 1 cut(s) 1056
PsuI RGATCY 1 cut(s) 1447
RsaI GTAC 7 cut(s) 39, 890, 1599, 1743, 1793, 2006, 2047
RsaNI GTAC 7 cut(s) 38, 889, 1598, 1742, 1792, 2005, 2046
RseI CAYNNNNRTG 1 cut(s) 1755
Rsr2I CGGWCCG 1 cut(s) 312
RsrII CGGWCCG 1 cut(s) 312
SacI GAGCTC 1 cut(s) 631
SaqAI TTAA 7 cut(s) 306, 1073, 1269, 1473, 1854, 2064, 2117
SatI GCNGC 6 cut(s) 473, 728, 969, 981, 1265, 1666
Sau3AI GATC 5 cut(s) 478, 801, 1408, 1447, 1618
Sau96I GGNCC 9 cut(s) 180, 217, 249, 255, 285, 292, 312, 349, 1882
ScaI AGTACT 1 cut(s) 1743
ScrFI CCNGG 6 cut(s) 215, 253, 1051, 1366, 1655, 1886
SduI GDGCHC 3 cut(s) 631, 1189, 2059
SexAI ACCWGGT 1 cut(s) 1653
Sfr274I CTCGAG 1 cut(s) 338
SinI GGWCC 4 cut(s) 180, 217, 312, 1882
SlaI CTCGAG 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 1755
SmlI CTYRAG 4 cut(s) 338, 630, 1564, 2108
SmoI CTYRAG 4 cut(s) 338, 630, 1564, 2108
SphI GCATGC 1 cut(s) 2096
Sse9I AATT 6 cut(s) 1293, 1351, 1442, 1553, 1981, 2118
SspMI CTAG 7 cut(s) 1493, 1635, 1680, 1781, 2072, 2139, 2143
SstI GAGCTC 1 cut(s) 631
StyD4I CCNGG 6 cut(s) 213, 251, 1049, 1364, 1653, 1884
StyI CCWWGG 1 cut(s) 1464
TaaI ACNGT 7 cut(s) 262, 581, 991, 1164, 1201, 1741, 2054
TaiI ACGT 4 cut(s) 98, 786, 896, 1716
TaqI TCGA 6 cut(s) 339, 537, 815, 1007, 1251, 1429
TasI AATT 6 cut(s) 1293, 1351, 1442, 1553, 1981, 2118
TatI WGTACW 3 cut(s) 1741, 1791, 2004
TauI GCSGC 3 cut(s) 971, 983, 1668
TfiI GAWTC 6 cut(s) 244, 343, 701, 949, 1747, 2135
Tru1I TTAA 7 cut(s) 306, 1073, 1269, 1473, 1854, 2064, 2117
Tru9I TTAA 7 cut(s) 306, 1073, 1269, 1473, 1854, 2064, 2117
TscAI CASTG 1 cut(s) 2059
TseFI GTSAC 3 cut(s) 96, 784, 1454
TseI GCWGC 3 cut(s) 472, 727, 1264
Tsp45I GTSAC 3 cut(s) 96, 784, 1454
TspDTI ATGAA 4 cut(s) 1092, 1634, 1739, 2090
TspGWI ACGGA 2 cut(s) 1195, 1410
TspRI CASTG 1 cut(s) 2059
Van91I CCANNNNNTGG 1 cut(s) 1056
VneI GTGCAC 1 cut(s) 2055
VpaK11BI GGWCC 4 cut(s) 180, 217, 312, 1882
VspI ATTAAT 1 cut(s) 1854
XagI CCTNNNNNAGG 1 cut(s) 1673
XapI RAATTY 2 cut(s) 1293, 1351
XceI RCATGY 3 cut(s) 412, 1585, 2096
XcmI CCANNNNNNNNNTGG 1 cut(s) 508
XhoI CTCGAG 1 cut(s) 338
XmiI GTMKAC 3 cut(s) 405, 1924, 1940
XspI CTAG 7 cut(s) 1493, 1635, 1680, 1781, 2072, 2139, 2143
ZrmI AGTACT 1 cut(s) 1743
Zsp2I ATGCAT 2 cut(s) 414, 1762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.