Rroxscaffold_5G00375450

Pentatricopeptide repeat-containing protein At1g22960

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011481
Physical Location & Seq
Forward (+)
56236441 .. 56238599
2159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375450.1

Sequence Viewer

Length: 2133 bp
ATGACCCTCTGCGCCAGAGCTTGTAAAGCTTTCAAGGTACGCCTCCCTCCTCTACCCCTCTCCTCCTCCTCCTCCTCCTCTTCCTTCCACTCTCCTTCCCTCCCCGCCACGTCACCTCTCTCCGACACCCACTACCGCGACCTCATCTTCTCCACCATCCGAACAACCCCCTCCGCTTTCCGCACCCGCGACTGGTCCTCCCACCGCTTCCGCCCCGTGATTTCCGACCCGGACCTCTTCGTCCGCGTCCTCGCCCAGATTCGGGCCAGGCCCACCGTCGCCCTCCGCTTCTTCCGATGGGCCGAGGCCCAGCCGGAGGTTAAGCGCTCCGAGTTTGTCTTCTGTGTCATTCTCGAGATTCTGGCCCACCACAACATGATGAGGTCTGCGTATTGGGTGATGGAGAGAGTGATTAGCGTAGATATGCATCATGGCATTGTGGATGTTTTGATTGATGAGTACGTTTTCGCAAAGGTCTCCATTAAGCTGCTTGATCTCTTGTTGTGGGTTTGTGCCAACAAGTTGTTGCTGGAGCAATGCTTGTCCATTTTCGATAAGATGATAACCAATGGGTTGTTGCCGGATGTGAATAACTGTAATAGGCTTCTTAGGATGCTTAGGGATAGGAACCTTGCCAGTAGAGCTAAAGAGGTTTATAGCAGGATGGGTGAGGCCGGGATCAAGCCCACCATTGTTACTTACAACACTATGTTGGATTCGTTTTGCAAGCAAGGGCAAGTGCAGCAGGCGCTTCACCTTGTGTCGGAGATGCAGAAGGTGGACTGCTTCCCGAATGATGTGACCTACAATGTGAAGCTGAAGCAGGCCGAGGGCCTGTTGCAAGATATGTTGAAGTCGGGATTGAGGGCTTCAGCCTACACATATAACCCTTTGATTTGTGGGTATTGCAAGAGGGGACTGCTTGCGGAGGCATTGAATCTCGGGGAAGAGATGGCGGCAAGAGGAGCCCCTCCTACTGTGCCTACTTATAACTCGATAATCTATGGCCTCTGCAAGTGGGGCAGAGTTACTGAGGCCAGGGACTGGTTTACTGATATGTTAAAGAAACGATTGTCGCCTGATATAGTTTCATACAACACTCTTATATATGGGTATTGTAGGTTAGGGGACATACGGAATGCTTTTCTACTGTTTGATGAGTTGAGACGGAGGGCCCTCACTCCTACTGTCGTTACTTATAATACACTCATGGAGGGTCTATGCAGATTGGGGGAGTTCGAGGTTGCTCAGCAGCTTAAAAAAGAAATGGTCAATCAAGGAACTTTCCCTGATGTTTTTACATATACTATTCTTGTAAATGGATATTGCAAGGCAGGAAATTTATCAAAGGCCAGGGAGTTATTTGATGAGATGTTGCATAACGGACTGGAGCCGGATCGCTTTGCATACAATACTCGAATAGTGGGGGAATTGGATCTTGGAGACCCATCCAAGGCATTTAATATGCAAGAAGAAATGCTAGCAAAGGGTTTGCCTCCTGATTTGTTCACATATAATGTTTTTGTGGATGGAATATGTAAATTGGGCAACTTGAGTGAAGCATATAACATGTTGCAGAAAATGGTACATGATGGTCTCGTGCCAGATCATGTAACATACACTAGCATCATTCATGCTTACCTGGTAAGTGGCCGTCTCAGGAAGGCTAGAGAGGTATATGATGAGATGCTGAGCAAAGACGTGCATCCCTCAGTTGTAACTTATACAGTACTGATTCATGCTCATGCATCTAAGGGGAGACTGGAACTAGCATATATGTACTTCTCAGAGATGCAAGAGAAGCGTGTTCTGCCAAATGTTGTTACCTACAATGCTCTGATAAATGGTCTTTGTAAAGTTATGAGAATGGACCAGGCTTATGAGTTCTTTACTCAGATGGAGGAAAAGGGTATACTTCCAAATAAGTATACATACACTATTCTAATAAATGAGAACTGCAACATCGGCAATTGGAAGGAGGCTTTGAGATTGTACAAACAGATGCTTGACAGAGAGATTGAACCTGACTTTTGTACGCACAGTGCACTTTTTAAGCAACTAGACAGAGACTATAAACTGCATGCAGTTCATTGCCTTGAGAGTTTAATTCTTGCCACAGAAGAATCTAGCTAG

Protein Analysis

710

Amino Acids

81.27

Weight (kDa)

8.13

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 166 - 273 4.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 182 - 241 1.1e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 194 - 243 1.2e-08 PPR repeat family
PPR_1 PF12854 226 - 258 4.1e-11 PPR repeat
PPR_2 PF13041 229 - 271 4.5e-15 PPR repeat family
PPR_3 PF13812 231 - 273 8.8e-06 Pentatricopeptide repeat domain
PPR PF01535 232 - 261 7.7e-07 PPR repeat
PPR_3 PF13812 279 - 334 1.8e-06 Pentatricopeptide repeat domain
TPR_24 PF23276 282 - 387 1.2e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 287 - 318 4e-08 PPR repeat
PPR_2 PF13041 292 - 339 3.3e-13 PPR repeat family
PPR_1 PF12854 321 - 353 7e-07 PPR repeat
PPR_2 PF13041 325 - 373 4e-14 PPR repeat family
PPR PF01535 329 - 357 2.9e-06 PPR repeat
PPR_3 PF13812 351 - 405 2.9e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 357 - 388 1.1e-10 PPR repeat
PPR_2 PF13041 360 - 409 1.5e-17 PPR repeat family
PPR PF01535 363 - 391 4e-07 PPR repeat
PPR_1 PF12854 393 - 424 1.9e-09 PPR repeat
PPR_2 PF13041 395 - 444 2.7e-18 PPR repeat family
PPR_3 PF13812 419 - 472 2.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 427 - 458 2.6e-14 PPR repeat
PPR PF01535 433 - 463 9.7e-10 PPR repeat
PPR_2 PF13041 437 - 472 3.7e-09 PPR repeat family
PPR_2 PF13041 465 - 514 8.6e-10 PPR repeat family
PPR_1 PF12854 496 - 529 1.5e-09 PPR repeat
PPR_2 PF13041 503 - 547 9.4e-11 PPR repeat family
PPR_3 PF13812 524 - 575 6.7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 531 - 563 1.2e-06 PPR repeat
PPR_2 PF13041 535 - 583 1.2e-14 PPR repeat family
PPR PF01535 538 - 567 6.9e-07 PPR repeat
PPR_long PF17177 553 - 663 5.7e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 560 - 615 4.6e-14 Pentatricopeptide repeat domain
PPR_1 PF12854 566 - 599 1e-05 PPR repeat
PPR_2 PF13041 570 - 619 1.1e-17 PPR repeat family
PPR PF01535 573 - 602 3.4e-06 PPR repeat
PPR_1 PF12854 602 - 634 6.2e-13 PPR repeat
PPR_2 PF13041 605 - 654 6.6e-20 PPR repeat family
PPR PF01535 608 - 638 8.9e-08 PPR repeat
PPR_3 PF13812 630 - 685 1.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 636 - 668 1.5e-07 PPR repeat
PPR_2 PF13041 648 - 687 6.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22960 AT1G22960
fragaria_vesca FvH4_4g26410
malus_domestica MD13G1032800.v1.1 MD16G1034800.v1.1
pyrus_communis pycom13g02830 pycom16g02960
rosa_chinensis RchiOBHm_Chr4g0434041
rosa_laevigata RLG00000006706
rosa_multiflora Rmu_co8498505.1_g000001 Rmu_sc0000352.1_g000026
rosa_roxburghii Rroxscaffold_5G00375450
rosa_rugosa Rorug04G0272900
rosa_samantha Rh4AG328600 Rh4BG335900 Rh4CG351400 Rh4DG332300 Rh5AG231100 Rh5BG086200
rosa_wichuraiana Rw4G028490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 990, 1200
AasI GACNNNNNNGTC 1 cut(s) 239
AccB7I CCANNNNNTGG 1 cut(s) 1044
AccI GTMKAC 2 cut(s) 1912, 1928
AccII CGCG 3 cut(s) 138, 189, 246
AclWI GGATC 3 cut(s) 686, 1404, 1443
AcoI YGGCCR 1 cut(s) 1651
AcsI RAATTY 1 cut(s) 1339
AcuI CTGAAG 2 cut(s) 839, 855
AdeI CACNNNGTG 1 cut(s) 760
AfaI GTAC 7 cut(s) 39, 461, 1587, 1731, 1781, 1994, 2035
AfeI AGCGCT 1 cut(s) 326
AfiI CCNNNNNNNGG 7 cut(s) 192, 261, 262, 316, 763, 1044, 1453
AflIII ACRYGT 1 cut(s) 1569
AgsI TTSAA 4 cut(s) 34, 853, 937, 2021
AjiI CACGTC 2 cut(s) 111, 1702
AjnI CCWGG 5 cut(s) 266, 1037, 1352, 1641, 1872
AjuI GAANNNNNNNTTGG 2 cut(s) 1422, 1454
AluBI AGCT 7 cut(s) 20, 29, 487, 644, 817, 1255, 2130
AluI AGCT 7 cut(s) 20, 29, 487, 644, 817, 1255, 2130
Alw21I GWGCWC 1 cut(s) 2047
Alw26I GTCTC 7 cut(s) 481, 1159, 1437, 1601, 1661, 1753, 2061
Alw44I GTGCAC 1 cut(s) 2043
AlwI GGATC 3 cut(s) 686, 1404, 1443
AlwNI CAGNNNCTG 1 cut(s) 1044
Ama87I CYCGRG 2 cut(s) 353, 941
Aor51HI AGCGCT 1 cut(s) 326
ApaI GGGCCC 1 cut(s) 1177
ApaLI GTGCAC 1 cut(s) 2043
ApeKI GCWGC 3 cut(s) 487, 742, 1252
ApoI RAATTY 1 cut(s) 1339
AspLEI GCGC 3 cut(s) 14, 327, 751
AsuC2I CCSGG 2 cut(s) 230, 676
AsuHPI GGTGA 4 cut(s) 105, 409, 680, 746
AsuNHI GCTAGC 1 cut(s) 1480
AvaI CYCGRG 2 cut(s) 353, 941
AvaII GGWCC 3 cut(s) 195, 232, 1870
BaeGI GKGCMC 2 cut(s) 1177, 2047
BanII GRGCYC 2 cut(s) 970, 1177
BauI CACGAG 1 cut(s) 1598
BbsI GAAGAC 1 cut(s) 331
Bbv12I GWGCWC 1 cut(s) 2047
BbvI GCAGC 3 cut(s) 474, 754, 1264
BccI CCATC 9 cut(s) 164, 291, 394, 658, 946, 1456, 1523, 1586, 1891
BceAI ACGGC 1 cut(s) 1638
BciT130I CCWGG 5 cut(s) 268, 1039, 1354, 1643, 1874
BcnI CCSGG 2 cut(s) 230, 676
BcoDI GTCTC 7 cut(s) 481, 1159, 1437, 1601, 1661, 1753, 2061
BfaI CTAG 7 cut(s) 1481, 1623, 1668, 1769, 2060, 2127, 2131
BfoI RGCGCY 2 cut(s) 328, 752
BisI GCNGC 4 cut(s) 488, 743, 957, 1253
BlpI GCTNAGC 2 cut(s) 1248, 1691
BlsI GCNGC 4 cut(s) 489, 744, 958, 1254
BmcAI AGTACT 1 cut(s) 1731
Bme1390I CCNGG 7 cut(s) 230, 268, 676, 1039, 1354, 1643, 1874
Bme18I GGWCC 3 cut(s) 195, 232, 1870
BmeT110I CYCGRG 2 cut(s) 353, 941
BmgBI CACGTC 2 cut(s) 111, 1702
BmiI GGNNCC 4 cut(s) 629, 967, 1175, 1392
BmrFI CCNGG 7 cut(s) 230, 268, 676, 1039, 1354, 1643, 1874
BmsI GCATC 9 cut(s) 436, 603, 759, 1635, 1677, 1714, 1757, 1782, 1992
BmtI GCTAGC 1 cut(s) 1484
BpiI GAAGAC 1 cut(s) 331
BpmI CTGGAG 2 cut(s) 551, 1409
Bpu10I CCTNAGC 1 cut(s) 617
Bpu1102I GCTNAGC 2 cut(s) 1248, 1691
BpuEI CTTGAG 2 cut(s) 1573, 2117
BpuMI CCSGG 2 cut(s) 230, 676
BsaBI GATNNNNATC 1 cut(s) 426
BsaI GGTCTC 3 cut(s) 481, 1437, 1601
BsaJI CCNNGG 5 cut(s) 303, 828, 1038, 1353, 1452
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 7 cut(s) 192, 261, 262, 316, 763, 1044, 1453
Bse1I ACTGG 5 cut(s) 197, 636, 1049, 1392, 1767
Bse3DI GCAATG 2 cut(s) 542, 2089
Bse8I GATNNNNATC 1 cut(s) 426
BseBI CCWGG 5 cut(s) 268, 1039, 1354, 1643, 1874
BseDI CCNNGG 5 cut(s) 303, 828, 1038, 1353, 1452
BseGI GGATG 8 cut(s) 156, 448, 589, 618, 669, 1448, 1534, 1705
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 7 cut(s) 192, 261, 262, 316, 763, 1044, 1453
BseMI GCAATG 2 cut(s) 542, 2089
BseMII CTCAG 7 cut(s) 1023, 1262, 1672, 1682, 1725, 1800, 1907
BseNI ACTGG 5 cut(s) 197, 636, 1049, 1392, 1767
BseRI GAGGAG 8 cut(s) 39, 52, 55, 58, 61, 64, 67, 978
BseSI GKGCMC 2 cut(s) 1177, 2047
BseXI GCAGC 3 cut(s) 474, 754, 1264
BseYI CCCAGC 1 cut(s) 309
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 3 cut(s) 138, 189, 246
BsiHKAI GWGCWC 1 cut(s) 2047
BsiHKCI CYCGRG 2 cut(s) 353, 941
BsiSI CCGG 5 cut(s) 230, 314, 581, 675, 1394
BslFI GGGAC 3 cut(s) 930, 1055, 1142
BslI CCNNNNNNNGG 7 cut(s) 192, 261, 262, 316, 763, 1044, 1453
BsmAI GTCTC 7 cut(s) 481, 1159, 1437, 1601, 1661, 1753, 2061
BsmBI CGTCTC 2 cut(s) 1159, 1661
BsmFI GGGAC 3 cut(s) 930, 1055, 1142
BsmI GAATGC 1 cut(s) 1144
Bso31I GGTCTC 3 cut(s) 481, 1437, 1601
BsoBI CYCGRG 2 cut(s) 353, 941
Bsp120I GGGCCC 1 cut(s) 1173
Bsp1286I GDGCHC 3 cut(s) 970, 1177, 2047
Bsp1407I TGTACA 1 cut(s) 1992
Bsp143I GATC 5 cut(s) 493, 678, 1396, 1435, 1606
Bsp1720I GCTNAGC 2 cut(s) 1248, 1691
BspCNI CTCAG 7 cut(s) 1024, 1261, 1671, 1683, 1724, 1799, 1906
BspFNI CGCG 3 cut(s) 138, 189, 246
BspLI GGNNCC 4 cut(s) 629, 967, 1175, 1392
BspOI GCTAGC 1 cut(s) 1484
BspPI GGATC 3 cut(s) 686, 1404, 1443
BspTNI GGTCTC 3 cut(s) 481, 1437, 1601
BsrDI GCAATG 2 cut(s) 542, 2089
BsrGI TGTACA 1 cut(s) 1992
BsrI ACTGG 5 cut(s) 197, 636, 1049, 1392, 1767
BssECI CCNNGG 5 cut(s) 303, 828, 1038, 1353, 1452
BssMI GATC 5 cut(s) 493, 678, 1396, 1435, 1606
BssNAI GTATAC 2 cut(s) 1913, 1929
BssSI CACGAG 1 cut(s) 1598
BssT1I CCWWGG 1 cut(s) 1452
Bst1107I GTATAC 2 cut(s) 1913, 1929
Bst2BI CACGAG 1 cut(s) 1598
Bst2UI CCWGG 5 cut(s) 268, 1039, 1354, 1643, 1874
Bst4CI ACNGT 7 cut(s) 277, 596, 979, 1152, 1189, 1729, 2042
Bst6I CTCTTC 3 cut(s) 85, 242, 942
BstAUI TGTACA 1 cut(s) 1992
BstC8I GCNNGC 6 cut(s) 728, 747, 825, 924, 1482, 2082
BstF5I GGATG 8 cut(s) 156, 448, 589, 618, 669, 1448, 1534, 1705
BstFNI CGCG 3 cut(s) 138, 189, 246
BstH2I RGCGCY 2 cut(s) 328, 752
BstHHI GCGC 3 cut(s) 14, 327, 751
BstKTI GATC 5 cut(s) 496, 681, 1399, 1438, 1609
BstMAI GTCTC 7 cut(s) 481, 1159, 1437, 1601, 1661, 1753, 2061
BstMBI GATC 5 cut(s) 493, 678, 1396, 1435, 1606
BstMWI GCNNNNNNNGC 9 cut(s) 26, 641, 742, 748, 965, 1020, 1801, 1810, 1965
BstNI CCWGG 5 cut(s) 268, 1039, 1354, 1643, 1874
BstNSI RCATGY 2 cut(s) 1573, 2084
BstSCI CCNGG 7 cut(s) 228, 266, 674, 1037, 1352, 1641, 1872
BstSLI GKGCMC 2 cut(s) 1177, 2047
BstUI CGCG 3 cut(s) 138, 189, 246
BstV1I GCAGC 3 cut(s) 474, 754, 1264
BstV2I GAAGAC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 1435
BstYI RGATCY 1 cut(s) 1435
BstZ17I GTATAC 2 cut(s) 1913, 1929
BtrI CACGTC 2 cut(s) 111, 1702
BtsCI GGATG 8 cut(s) 156, 448, 589, 618, 669, 1448, 1534, 1705
BtsIMutI CAGTG 1 cut(s) 2047
Cac8I GCNNGC 6 cut(s) 728, 747, 825, 924, 1482, 2082
CaiI CAGNNNCTG 1 cut(s) 1044
CfoI GCGC 3 cut(s) 14, 327, 751
CseI GACGC 1 cut(s) 235
CsiI ACCWGGT 1 cut(s) 1641
Csp6I GTAC 7 cut(s) 38, 460, 1586, 1730, 1780, 1993, 2034
CviQI GTAC 7 cut(s) 38, 460, 1586, 1730, 1780, 1993, 2034
DpnI GATC 5 cut(s) 495, 680, 1398, 1437, 1608
DpnII GATC 5 cut(s) 493, 678, 1396, 1435, 1606
DraIII CACNNNGTG 1 cut(s) 760
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 1651
Eam1104I CTCTTC 3 cut(s) 85, 242, 942
EarI CTCTTC 3 cut(s) 85, 242, 942
EciI GGCGGA 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 1452
Eco24I GRGCYC 2 cut(s) 970, 1177
Eco31I GGTCTC 3 cut(s) 481, 1437, 1601
Eco47I GGWCC 3 cut(s) 195, 232, 1870
Eco47III AGCGCT 1 cut(s) 326
Eco57I CTGAAG 2 cut(s) 839, 855
Eco88I CYCGRG 2 cut(s) 353, 941
EcoO109I RGGNCCY 3 cut(s) 832, 1173, 1174
EcoRII CCWGG 5 cut(s) 266, 1037, 1352, 1641, 1872
EcoT14I CCWWGG 1 cut(s) 1452
EcoT22I ATGCAT 2 cut(s) 429, 1750
EcoT38I GRGCYC 2 cut(s) 970, 1177
ErhI CCWWGG 1 cut(s) 1452
Esp3I CGTCTC 2 cut(s) 1159, 1661
FaqI GGGAC 3 cut(s) 930, 1055, 1142
FauI CCCGC 2 cut(s) 112, 194
FblI GTMKAC 2 cut(s) 1912, 1928
Fnu4HI GCNGC 4 cut(s) 488, 743, 957, 1253
FokI GGATG 8 cut(s) 143, 455, 596, 625, 676, 1435, 1541, 1692
FriOI GRGCYC 2 cut(s) 970, 1177
Fsp4HI GCNGC 4 cut(s) 488, 743, 957, 1253
FspBI CTAG 7 cut(s) 1481, 1623, 1668, 1769, 2060, 2127, 2131
GlaI GCGC 3 cut(s) 13, 326, 750
GluI GCNGC 4 cut(s) 488, 743, 957, 1253
GsaI CCCAGC 1 cut(s) 313
GsuI CTGGAG 2 cut(s) 551, 1409
HaeII RGCGCY 2 cut(s) 328, 752
HapII CCGG 5 cut(s) 230, 314, 581, 675, 1394
HgaI GACGC 1 cut(s) 235
HhaI GCGC 3 cut(s) 14, 327, 751
Hin6I GCGC 3 cut(s) 12, 325, 749
HinP1I GCGC 3 cut(s) 12, 325, 749
HindIII AAGCTT 1 cut(s) 27
HinfI GANTC 6 cut(s) 259, 358, 716, 937, 1735, 2123
HpaII CCGG 5 cut(s) 230, 314, 581, 675, 1394
HphI GGTGA 4 cut(s) 105, 409, 680, 746
Hpy166II GTNNAC 6 cut(s) 781, 1050, 1509, 1913, 1929, 2045
Hpy188I TCNGA 9 cut(s) 124, 161, 226, 296, 331, 766, 1789, 1839, 1896
Hpy188III TCNNGA 6 cut(s) 353, 355, 790, 858, 1499, 1660
Hpy8I GTNNAC 6 cut(s) 781, 1050, 1509, 1913, 1929, 2045
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 5 cut(s) 94, 105, 769, 1657, 1969
HpyCH4III ACNGT 7 cut(s) 277, 596, 979, 1152, 1189, 1729, 2042
HpyCH4IV ACGT 3 cut(s) 110, 462, 1701
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 641, 742, 748, 965, 1020, 1801, 1810, 1965
HpySE526I ACGT 3 cut(s) 110, 462, 1701
HspAI GCGC 3 cut(s) 12, 325, 749
Kzo9I GATC 5 cut(s) 493, 678, 1396, 1435, 1606
LmnI GCTCC 4 cut(s) 332, 532, 965, 1390
Lsp1109I GCAGC 3 cut(s) 474, 754, 1264
LweI GCATC 9 cut(s) 436, 603, 759, 1635, 1677, 1714, 1757, 1782, 1992
MabI ACCWGGT 1 cut(s) 1641
MaeI CTAG 7 cut(s) 1481, 1623, 1668, 1769, 2060, 2127, 2131
MaeII ACGT 3 cut(s) 110, 462, 1701
MaeIII GTNAC 8 cut(s) 111, 694, 799, 1027, 1192, 1612, 1717, 1822
MalI GATC 5 cut(s) 495, 680, 1398, 1437, 1608
MboI GATC 5 cut(s) 493, 678, 1396, 1435, 1606
MboII GAAGA 8 cut(s) 72, 139, 229, 283, 331, 959, 1484, 2132
MfeI CAATTG 1 cut(s) 1969
MflI RGATCY 1 cut(s) 1435
MhlI GDGCHC 3 cut(s) 970, 1177, 2047
MluCI AATT 5 cut(s) 1339, 1430, 1541, 1969, 2106
MmeI TCCRAC 4 cut(s) 147, 249, 693, 744
Mph1103I ATGCAT 2 cut(s) 429, 1750
MseI TTAA 7 cut(s) 321, 483, 1061, 1257, 1461, 2052, 2105
MslI CAYNNNNRTG 1 cut(s) 1743
MspI CCGG 5 cut(s) 230, 314, 581, 675, 1394
MspR9I CCNGG 7 cut(s) 230, 268, 676, 1039, 1354, 1643, 1874
MunI CAATTG 1 cut(s) 1969
Mva1269I GAATGC 1 cut(s) 1144
MvaI CCWGG 5 cut(s) 268, 1039, 1354, 1643, 1874
MvnI CGCG 3 cut(s) 138, 189, 246
MwoI GCNNNNNNNGC 9 cut(s) 26, 641, 742, 748, 965, 1020, 1801, 1810, 1965
NciI CCSGG 2 cut(s) 230, 676
NdeII GATC 5 cut(s) 493, 678, 1396, 1435, 1606
NheI GCTAGC 1 cut(s) 1480
NlaIV GGNNCC 4 cut(s) 629, 967, 1175, 1392
NmeAIII GCCGAG 2 cut(s) 328, 853
NmuCI GTSAC 2 cut(s) 111, 799
NsiI ATGCAT 2 cut(s) 429, 1750
NspI RCATGY 2 cut(s) 1573, 2084
PaeI GCATGC 1 cut(s) 2084
PaeR7I CTCGAG 1 cut(s) 353
PciI ACATGT 1 cut(s) 1569
PctI GAATGC 1 cut(s) 1144
PfeI GAWTC 6 cut(s) 259, 358, 716, 937, 1735, 2123
PflMI CCANNNNNTGG 1 cut(s) 1044
PkrI GCNGC 4 cut(s) 489, 744, 958, 1254
PscI ACATGT 1 cut(s) 1569
PsiI TTATAA 2 cut(s) 990, 1200
Psp6I CCWGG 5 cut(s) 266, 1037, 1352, 1641, 1872
PspFI CCCAGC 1 cut(s) 309
PspGI CCWGG 5 cut(s) 266, 1037, 1352, 1641, 1872
PspN4I GGNNCC 4 cut(s) 629, 967, 1175, 1392
PspOMI GGGCCC 1 cut(s) 1173
PstNI CAGNNNCTG 1 cut(s) 1044
PsuI RGATCY 1 cut(s) 1435
RsaI GTAC 7 cut(s) 39, 461, 1587, 1731, 1781, 1994, 2035
RsaNI GTAC 7 cut(s) 38, 460, 1586, 1730, 1780, 1993, 2034
RseI CAYNNNNRTG 1 cut(s) 1743
SaqAI TTAA 7 cut(s) 321, 483, 1061, 1257, 1461, 2052, 2105
SatI GCNGC 4 cut(s) 488, 743, 957, 1253
Sau3AI GATC 5 cut(s) 493, 678, 1396, 1435, 1606
ScaI AGTACT 1 cut(s) 1731
ScrFI CCNGG 7 cut(s) 230, 268, 676, 1039, 1354, 1643, 1874
SduI GDGCHC 3 cut(s) 970, 1177, 2047
SexAI ACCWGGT 1 cut(s) 1641
SfaNI GCATC 9 cut(s) 436, 603, 759, 1635, 1677, 1714, 1757, 1782, 1992
Sfr274I CTCGAG 1 cut(s) 353
SinI GGWCC 3 cut(s) 195, 232, 1870
SlaI CTCGAG 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 1743
SmlI CTYRAG 3 cut(s) 353, 1552, 2096
SmoI CTYRAG 3 cut(s) 353, 1552, 2096
SphI GCATGC 1 cut(s) 2084
Sse9I AATT 5 cut(s) 1339, 1430, 1541, 1969, 2106
SspMI CTAG 7 cut(s) 1481, 1623, 1668, 1769, 2060, 2127, 2131
StyD4I CCNGG 7 cut(s) 228, 266, 674, 1037, 1352, 1641, 1872
StyI CCWWGG 1 cut(s) 1452
TaaI ACNGT 7 cut(s) 277, 596, 979, 1152, 1189, 1729, 2042
TaiI ACGT 3 cut(s) 113, 465, 1704
TaqI TCGA 5 cut(s) 354, 552, 995, 1239, 1417
TasI AATT 5 cut(s) 1339, 1430, 1541, 1969, 2106
TatI WGTACW 3 cut(s) 1729, 1779, 1992
TauI GCSGC 1 cut(s) 959
TfiI GAWTC 6 cut(s) 259, 358, 716, 937, 1735, 2123
Tru1I TTAA 7 cut(s) 321, 483, 1061, 1257, 1461, 2052, 2105
Tru9I TTAA 7 cut(s) 321, 483, 1061, 1257, 1461, 2052, 2105
TscAI CASTG 1 cut(s) 2047
TseFI GTSAC 2 cut(s) 111, 799
TseI GCWGC 3 cut(s) 487, 742, 1252
Tsp45I GTSAC 2 cut(s) 111, 799
TspDTI ATGAA 4 cut(s) 1080, 1622, 1727, 2078
TspGWI ACGGA 3 cut(s) 1150, 1183, 1398
TspRI CASTG 1 cut(s) 2047
Van91I CCANNNNNTGG 1 cut(s) 1044
VneI GTGCAC 1 cut(s) 2043
VpaK11BI GGWCC 3 cut(s) 195, 232, 1870
XapI RAATTY 1 cut(s) 1339
XceI RCATGY 2 cut(s) 1573, 2084
XhoI CTCGAG 1 cut(s) 353
XmiI GTMKAC 2 cut(s) 1912, 1928
XspI CTAG 7 cut(s) 1481, 1623, 1668, 1769, 2060, 2127, 2131
ZrmI AGTACT 1 cut(s) 1731
Zsp2I ATGCAT 2 cut(s) 429, 1750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.