Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0011481

Physical Location & Seq

Genomic Coordinates Forward (+)

1859085 .. 1860848

Gene Structure 1764 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g02960.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGAGGCCGGCGCATTGGGTTATGGAGAGGGTGATTAGTGTAAATATGCATGGGATTGTGGATGTTTTGATTAATGAGTATGTGTTTTCGAAGGTCTCGTTGAAGCTTCTCGATCTCTTGTTCTGGGTTTATACAAAAAAATCGATGCTTGAGCAATGTCTCTGGATTTTCGATAAGATGGTTCGAAACCGGTTGTTGCCAGACGTGAAGAACTGCAATAGGGTTCTTAGGATACTTAGAAATAAACACCTTGTGACTAGAGTTAAAGACGTTTACAGAATGATGGGCGAGGCTGGGATTAAGCCGACGATTGTTACCTATAACACTATGTTGGATTCATTCTGCAAGGAAGGAGAAGTGCAACAAGCACTTGACCTTTTGTCAGAGATGCAAAAGATAGAGTGTTTTCCGAACGATGTAACGTATAATGTGTTGATCAATGGGCTGTCAAAGAAAGGGGAGCTGGAGCAGGCCAAGGGACTGATCCAGGAAATGATGAAATCTGGATTGAGGATTACAGCATTTACTTATAACCCTTTGATTTGTGGGTATTGCAACAAAGGGCTGCTTGAAGAGGCATTATGTCTTGAGAAAGAAATGGTAATTAGAGGAGCGAATCCCACCGTGGCTACTTATAATTCGTTAATGTATGGACTCTGCAAGCGGGGAAGAGTGATTGATGCCAGGGAACAGTTTTCCAATATGTTAAAGAGAAATATAAGGCCGGATATAGTGTCCTACAACACTCTAATATACGGGTATTGTAGATTAGGGAACTTGGGGGATGCTTTCAGATTGTTTGATGAGTTGAGGCATAGGACTTTCACTCCTACTGTCGTCACCTATAACACACTTATGGATGGTCTTTGCAGATCCGGAGACTTGGCTGTTGCTCGACAGCTTAAAAATGAAATGACCAATCAAGGAACTTGCCCTGATGTTTTTACATATACCATCTTGGTAAATGGATCTTGCAAGGCGGGAAACTTATCGATGGCCAAGGAGTTATTTGATGAGATGTTGCATAGAGGAGTGAAGCCGGACCGATTTGCATACAATACTCGAATAGTGGGTGAATTGACGCTTGGTGACCCTTCCAAGGCATTTAGCATGCAAGAAGAAATACTGGCAGGGGGTTTCCCTCCTGATTTGTTCACATACAATATTTTTGTGAATGGGATTTGTAAATTGGGCAATTTGGATGAAGCATATACCTTGTTGCAGAAAATTGTACGTGATGGTATCGTTCCAGACCATATAACTTATACTAGCATCATTCACGCTCACCTGGAGAGTGGCCAACTCATGAAGGCAAGAGAGGTGTTCTATGAGATGCTTAGCAAAGGTTTGTCTCCTTCGGTGATAACTTACACAGTATTGATTCATGCACACGCAGCTAAGGGAAGACTAGAACTGGCATATATGTACTTCTCAGAGATGCAAGAGAAGCGTGTTTTGCCAAATGTGGTCACCTACAATGCGCTGATAAATGGCCTTTGTAAAGTGATGAGAATGGATCAGGCTTACAAGTCGTTTGCGGAGATGGAGGAGAAAGGAATAACTCCAAATAAGTATACATACACTATTCTAATAAATGAGAACTGCAACATAGGCAACTGGAAGGAGGCTTTGAGGTTGTATAAACAGATGCTTGACAGATCGATCGAGCCTGATTCTTGTACACACAGTGCACTTTTTAAGCATCTAGACAAAGACTATCAGTTGCATGCAGTTCGGTACCTTGAGAATTTAATTTCAAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

588

Amino Acids

67.24

Weight (kDa)

8.51

Isoelectric Point (pI)

28.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	56 - 114	4.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	67 - 116	1.9e-07	PPR repeat family
TPR_24	PF23276	73 - 171	3.1e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	94 - 147	1.3e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	99 - 131	2e-12	PPR repeat
PPR_2	PF13041	102 - 151	5.9e-19	PPR repeat family
PPR	PF01535	105 - 133	3.5e-08	PPR repeat
PPR_long	PF17177	126 - 221	1.1e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	135 - 165	1.5e-08	PPR repeat
PPR_2	PF13041	137 - 183	2.8e-12	PPR repeat family
PPR	PF01535	140 - 170	6.8e-08	PPR repeat
PPR_3	PF13812	161 - 216	8.3e-06	Pentatricopeptide repeat domain
TPR_24	PF23276	162 - 269	7.1e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	169 - 201	1.7e-07	PPR repeat
PPR_2	PF13041	174 - 221	6.1e-14	PPR repeat family
PPR_1	PF12854	203 - 235	4.9e-07	PPR repeat
PPR_2	PF13041	207 - 255	1.5e-14	PPR repeat family
PPR_3	PF13812	233 - 287	6.2e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	238 - 270	1.9e-12	PPR repeat
PPR_2	PF13041	242 - 291	2.8e-18	PPR repeat family
PPR	PF01535	245 - 272	1.8e-07	PPR repeat
PPR_1	PF12854	275 - 306	8.3e-11	PPR repeat
PPR_2	PF13041	277 - 326	8.1e-18	PPR repeat family
PPR_3	PF13812	301 - 354	6.6e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	309 - 340	3.9e-12	PPR repeat
PPR	PF01535	315 - 345	8.3e-08	PPR repeat
PPR_2	PF13041	322 - 354	2.6e-07	PPR repeat family
PPR_2	PF13041	350 - 396	2.3e-07	PPR repeat family
PPR_1	PF12854	379 - 409	1.8e-09	PPR repeat
PPR_2	PF13041	382 - 428	1.7e-11	PPR repeat family
PPR_3	PF13812	406 - 461	8.1e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	417 - 465	2.9e-13	PPR repeat family
PPR	PF01535	420 - 450	9.8e-06	PPR repeat
PPR_long	PF17177	435 - 550	1.1e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	444 - 497	3.5e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	448 - 481	7.6e-06	PPR repeat
PPR_2	PF13041	452 - 501	3.6e-17	PPR repeat family
PPR_1	PF12854	484 - 516	1.8e-11	PPR repeat
PPR_2	PF13041	487 - 536	4.5e-20	PPR repeat family
PPR	PF01535	490 - 520	4.1e-07	PPR repeat
PPR_3	PF13812	512 - 567	2.6e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	518 - 550	8.4e-08	PPR repeat
PPR_2	PF13041	531 - 569	8.6e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011481)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22960 AT1G22960
fragaria_vesca	FvH4_4g26410
malus_domestica	MD13G1032800.v1.1 MD16G1034800.v1.1
pyrus_communis	pycom13g02830 pycom16g02960
rosa_chinensis	RchiOBHm_Chr4g0434041
rosa_laevigata	RLG00000006706
rosa_multiflora	Rmu_co8498505.1_g000001 Rmu_sc0000352.1_g000026
rosa_roxburghii	Rroxscaffold_5G00375450
rosa_rugosa	Rorug04G0272900
rosa_samantha	Rh4AG328600 Rh4BG335900 Rh4CG351400 Rh4DG332300 Rh5AG231100 Rh5BG086200
rosa_wichuraiana	Rw4G028490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	531, 636
Acc65I	GGTACC	1 cut(s)	1737
AccB1I	GGYRCC	1 cut(s)	1737
AccI	GTMKAC	1 cut(s)	1574
AccIII	TCCGGA	1 cut(s)	875
AciI	CCGC	3 cut(s)	664, 980, 1538
AclWI	GGATC	4 cut(s)	478, 867, 976, 1524
AcoI	YGGCCR	2 cut(s)	996, 1297
AcsI	RAATTY	1 cut(s)	1747
AdeI	CACNNNGTG	2 cut(s)	253, 1688
AfaI	GTAC	4 cut(s)	1233, 1427, 1681, 1739
AfiI	CCNNNNNNNGG	1 cut(s)	1099
AgeI	ACCGGT	1 cut(s)	189
AgsI	TTSAA	3 cut(s)	103, 572, 1758
AhdI	GACNNNNNGTC	1 cut(s)	379
AjiI	CACGTC	1 cut(s)	205
AjnI	CCWGG	3 cut(s)	486, 683, 1287
AjuI	GAANNNNNNNTTGG	2 cut(s)	1068, 1100
AluBI	AGCT	5 cut(s)	106, 463, 901, 1397, 1761
AluI	AGCT	5 cut(s)	106, 463, 901, 1397, 1761
Alw21I	GWGCWC	1 cut(s)	1693
Alw26I	GTCTC	4 cut(s)	100, 164, 873, 1356
Alw44I	GTGCAC	1 cut(s)	1689
AlwI	GGATC	4 cut(s)	478, 867, 976, 1524
Aor13HI	TCCGGA	1 cut(s)	875
AoxI	GGCC	6 cut(s)	5, 471, 722, 996, 1297, 1492
ApaLI	GTGCAC	1 cut(s)	1689
ApeKI	GCWGC	2 cut(s)	565, 1394
ApoI	RAATTY	1 cut(s)	1747
AseI	ATTAAT	1 cut(s)	72
AsiGI	ACCGGT	1 cut(s)	189
Asp718I	GGTACC	1 cut(s)	1737
AspLEI	GCGC	2 cut(s)	13, 1483
AspS9I	GGNCC	1 cut(s)	1042
AsuHPI	GGTGA	7 cut(s)	43, 832, 1085, 1100, 1277, 1372, 1462
AsuII	TTCGAA	2 cut(s)	89, 184
AvaII	GGWCC	1 cut(s)	1042
BaeGI	GKGCMC	1 cut(s)	1693
BalI	TGGCCA	2 cut(s)	998, 1299
BanI	GGYRCC	1 cut(s)	1737
BbsI	GAAGAC	1 cut(s)	1411
Bbv12I	GWGCWC	1 cut(s)	1693
BbvI	GCAGC	2 cut(s)	552, 1406
BccI	CCATC	7 cut(s)	172, 277, 854, 962, 988, 1232, 1537
BcgI	CGANNNNNNTGC	2 cut(s)	1715, 1749
BciT130I	CCWGG	3 cut(s)	488, 685, 1289
BciVI	GTATCC	1 cut(s)	225
BclI	TGATCA	1 cut(s)	435
BcoDI	GTCTC	4 cut(s)	100, 164, 873, 1356
BfaI	CTAG	5 cut(s)	258, 1269, 1409, 1706, 1762
BfuI	GTATCC	1 cut(s)	225
BisI	GCNGC	2 cut(s)	566, 1395
BlpI	GCTNAGC	1 cut(s)	1337
BlsI	GCNGC	2 cut(s)	567, 1396
Bme1390I	CCNGG	3 cut(s)	488, 685, 1289
Bme18I	GGWCC	1 cut(s)	1042
BmeRI	GACNNNNNGTC	1 cut(s)	379
BmgBI	CACGTC	1 cut(s)	205
BmgT120I	GGNCC	1 cut(s)	1042
BmiI	GGNNCC	1 cut(s)	1739
BmrFI	CCNGG	3 cut(s)	488, 685, 1289
BmsI	GCATC	9 cut(s)	135, 378, 670, 775, 1281, 1323, 1428, 1638, 1711
BpiI	GAAGAC	1 cut(s)	1411
BpmI	CTGGAG	2 cut(s)	485, 1310
Bpu10I	CCTNAGC	1 cut(s)	1398
Bpu1102I	GCTNAGC	1 cut(s)	1337
Bpu14I	TTCGAA	2 cut(s)	89, 184
BpuEI	CTTGAG	3 cut(s)	170, 608, 1763
Bsa29I	ATCGAT	3 cut(s)	143, 992, 1661
BsaAI	YACGTR	1 cut(s)	1235
BsaI	GGTCTC	1 cut(s)	100
BsaJI	CCNNGG	5 cut(s)	474, 624, 684, 999, 1098
BsaWI	WCCGGW	2 cut(s)	189, 875
BsaXI	ACNNNNNCTCC	2 cut(s)	811, 841
Bsc4I	CCNNNNNNNGG	1 cut(s)	1099
Bse118I	RCCGGY	2 cut(s)	7, 189
Bse1I	ACTGG	3 cut(s)	1131, 1419, 1622
Bse3DI	GCAATG	1 cut(s)	161
BseAI	TCCGGA	1 cut(s)	875
BseBI	CCWGG	3 cut(s)	488, 685, 1289
BseCI	ATCGAT	3 cut(s)	143, 992, 1661
BseDI	CCNNGG	5 cut(s)	474, 624, 684, 999, 1098
BseGI	GGATG	4 cut(s)	67, 790, 865, 1207
BseLI	CCNNNNNNNGG	1 cut(s)	1099
BseMI	GCAATG	1 cut(s)	161
BseMII	CTCAG	1 cut(s)	1446
BseNI	ACTGG	3 cut(s)	1131, 1419, 1622
BseRI	GAGGAG	3 cut(s)	624, 1044, 1562
BseSI	GKGCMC	1 cut(s)	1693
BseXI	GCAGC	2 cut(s)	552, 1406
BseYI	CCCAGC	1 cut(s)	293
Bsh1285I	CGRYCG	1 cut(s)	1665
BshFI	GGCC	6 cut(s)	7, 473, 724, 998, 1299, 1494
BshNI	GGYRCC	1 cut(s)	1737
BshTI	ACCGGT	1 cut(s)	189
BshVI	ATCGAT	3 cut(s)	143, 992, 1661
BsiEI	CGRYCG	1 cut(s)	1665
BsiHKAI	GWGCWC	1 cut(s)	1693
BsiSI	CCGG	5 cut(s)	8, 190, 725, 876, 1040
BslFI	GGGAC	1 cut(s)	492
BslI	CCNNNNNNNGG	1 cut(s)	1099
BsmAI	GTCTC	4 cut(s)	100, 164, 873, 1356
BsmFI	GGGAC	1 cut(s)	492
BsnI	GGCC	6 cut(s)	7, 473, 724, 998, 1299, 1494
Bso31I	GGTCTC	1 cut(s)	100
Bsp119I	TTCGAA	2 cut(s)	89, 184
Bsp1286I	GDGCHC	1 cut(s)	1693
Bsp13I	TCCGGA	1 cut(s)	875
Bsp1407I	TGTACA	1 cut(s)	1679
Bsp143I	GATC	8 cut(s)	112, 435, 483, 872, 968, 1516, 1658, 1662
Bsp1720I	GCTNAGC	1 cut(s)	1337
BspACI	CCGC	3 cut(s)	664, 980, 1538
BspANI	GGCC	6 cut(s)	7, 473, 724, 998, 1299, 1494
BspCNI	CTCAG	1 cut(s)	1445
BspDI	ATCGAT	3 cut(s)	143, 992, 1661
BspEI	TCCGGA	1 cut(s)	875
BspHI	TCATGA	1 cut(s)	1305
BspLI	GGNNCC	1 cut(s)	1739
BspPI	GGATC	4 cut(s)	478, 867, 976, 1524
BspT104I	TTCGAA	2 cut(s)	89, 184
BspT107I	GGYRCC	1 cut(s)	1737
BspTNI	GGTCTC	1 cut(s)	100
BsrDI	GCAATG	1 cut(s)	161
BsrFI	RCCGGY	2 cut(s)	7, 189
BsrGI	TGTACA	1 cut(s)	1679
BsrI	ACTGG	3 cut(s)	1131, 1419, 1622
BssAI	RCCGGY	2 cut(s)	7, 189
BssECI	CCNNGG	5 cut(s)	474, 624, 684, 999, 1098
BssMI	GATC	8 cut(s)	112, 435, 483, 872, 968, 1516, 1658, 1662
BssNAI	GTATAC	1 cut(s)	1575
BssT1I	CCWWGG	3 cut(s)	474, 999, 1098
Bst1107I	GTATAC	1 cut(s)	1575
Bst2UI	CCWGG	3 cut(s)	488, 685, 1289
Bst4CI	ACNGT	5 cut(s)	625, 693, 835, 1375, 1688
Bst6I	CTCTTC	2 cut(s)	567, 664
BstAUI	TGTACA	1 cut(s)	1679
BstBAI	YACGTR	1 cut(s)	1235
BstBI	TTCGAA	2 cut(s)	89, 184
BstC8I	GCNNGC	5 cut(s)	9, 471, 662, 1112, 1728
BstDEI	CTNAG	5 cut(s)	227, 236, 1337, 1398, 1432
BstDSI	CCRYGG	1 cut(s)	624
BstEII	GGTNACC	2 cut(s)	1088, 1468
BstF5I	GGATG	4 cut(s)	67, 790, 865, 1207
BstHHI	GCGC	2 cut(s)	13, 1483
BstKTI	GATC	8 cut(s)	115, 438, 486, 875, 971, 1519, 1661, 1665
BstMAI	GTCTC	4 cut(s)	100, 164, 873, 1356
BstMBI	GATC	8 cut(s)	112, 435, 483, 872, 968, 1516, 1658, 1662
BstMCI	CGRYCG	1 cut(s)	1665
BstMWI	GCNNNNNNNGC	4 cut(s)	1394, 1447, 1456, 1611
BstNI	CCWGG	3 cut(s)	488, 685, 1289
BstNSI	RCATGY	2 cut(s)	1114, 1730
BstPI	GGTNACC	2 cut(s)	1088, 1468
BstSCI	CCNGG	3 cut(s)	486, 683, 1287
BstSLI	GKGCMC	1 cut(s)	1693
BstV1I	GCAGC	2 cut(s)	552, 1406
BstV2I	GAAGAC	1 cut(s)	1411
BstX2I	RGATCY	2 cut(s)	872, 968
BstYI	RGATCY	2 cut(s)	872, 968
BstZ17I	GTATAC	1 cut(s)	1575
Bsu15I	ATCGAT	3 cut(s)	143, 992, 1661
BsuI	GTATCC	1 cut(s)	225
BsuRI	GGCC	6 cut(s)	7, 473, 724, 998, 1299, 1494
BsuTUI	ATCGAT	3 cut(s)	143, 992, 1661
BtgI	CCRYGG	1 cut(s)	624
BtrI	CACGTC	1 cut(s)	205
BtsCI	GGATG	4 cut(s)	67, 790, 865, 1207
BtsIMutI	CAGTG	1 cut(s)	1693
Cac8I	GCNNGC	5 cut(s)	9, 471, 662, 1112, 1728
CciI	TCATGA	1 cut(s)	1305
CfoI	GCGC	2 cut(s)	13, 1483
Cfr10I	RCCGGY	2 cut(s)	7, 189
Cfr13I	GGNCC	1 cut(s)	1042
ClaI	ATCGAT	3 cut(s)	143, 992, 1661
CpoI	CGGWCCG	1 cut(s)	1042
CseI	GACGC	1 cut(s)	1090
Csp6I	GTAC	4 cut(s)	1232, 1426, 1680, 1738
CspAI	ACCGGT	1 cut(s)	189
CspI	CGGWCCG	1 cut(s)	1042
CviAII	CATG	5 cut(s)	50, 1111, 1306, 1385, 1727
CviQI	GTAC	4 cut(s)	1232, 1426, 1680, 1738
DdeI	CTNAG	5 cut(s)	227, 236, 1337, 1398, 1432
DpnI	GATC	8 cut(s)	114, 437, 485, 874, 970, 1518, 1660, 1664
DpnII	GATC	8 cut(s)	112, 435, 483, 872, 968, 1516, 1658, 1662
DraIII	CACNNNGTG	2 cut(s)	253, 1688
DriI	GACNNNNNGTC	1 cut(s)	379
EaeI	YGGCCR	2 cut(s)	996, 1297
Eam1104I	CTCTTC	2 cut(s)	567, 664
Eam1105I	GACNNNNNGTC	1 cut(s)	379
EarI	CTCTTC	2 cut(s)	567, 664
Eco130I	CCWWGG	3 cut(s)	474, 999, 1098
Eco31I	GGTCTC	1 cut(s)	100
Eco47I	GGWCC	1 cut(s)	1042
Eco91I	GGTNACC	2 cut(s)	1088, 1468
EcoO65I	GGTNACC	2 cut(s)	1088, 1468
EcoRII	CCWGG	3 cut(s)	486, 683, 1287
EcoT14I	CCWWGG	3 cut(s)	474, 999, 1098
EcoT22I	ATGCAT	1 cut(s)	51
ErhI	CCWWGG	3 cut(s)	474, 999, 1098
FaeI	CATG	5 cut(s)	53, 1114, 1309, 1388, 1730
FalI	AAGNNNNNCTT	2 cut(s)	552, 584
FaqI	GGGAC	1 cut(s)	492
FatI	CATG	5 cut(s)	49, 1110, 1305, 1384, 1726
FauI	CCCGC	2 cut(s)	657, 973
FbaI	TGATCA	1 cut(s)	435
FblI	GTMKAC	1 cut(s)	1574
Fnu4HI	GCNGC	2 cut(s)	566, 1395
FokI	GGATG	4 cut(s)	74, 797, 872, 1214
Fsp4HI	GCNGC	2 cut(s)	566, 1395
FspBI	CTAG	5 cut(s)	258, 1269, 1409, 1706, 1762
GlaI	GCGC	2 cut(s)	12, 1482
GluI	GCNGC	2 cut(s)	566, 1395
GsaI	CCCAGC	1 cut(s)	297
GsuI	CTGGAG	2 cut(s)	485, 1310
HaeIII	GGCC	6 cut(s)	7, 473, 724, 998, 1299, 1494
HapII	CCGG	5 cut(s)	8, 190, 725, 876, 1040
HgaI	GACGC	1 cut(s)	1090
HhaI	GCGC	2 cut(s)	13, 1483
Hin1II	CATG	5 cut(s)	53, 1114, 1309, 1388, 1730
Hin6I	GCGC	2 cut(s)	11, 1481
HinP1I	GCGC	2 cut(s)	11, 1481
HindIII	AAGCTT	1 cut(s)	104
HinfI	GANTC	5 cut(s)	335, 616, 654, 1381, 1673
HpaII	CCGG	5 cut(s)	8, 190, 725, 876, 1040
HphI	GGTGA	7 cut(s)	43, 832, 1085, 1100, 1277, 1372, 1462
Hpy166II	GTNNAC	5 cut(s)	274, 1155, 1575, 1682, 1691
Hpy188I	TCNGA	4 cut(s)	385, 411, 794, 1435
Hpy188III	TCNNGA	9 cut(s)	110, 163, 504, 587, 876, 1145, 1250, 1306, 1706
Hpy8I	GTNNAC	5 cut(s)	274, 1155, 1575, 1682, 1691
Hpy99I	CGWCG	1 cut(s)	310
HpyAV	CCTTC	6 cut(s)	85, 344, 1104, 1303, 1365, 1615
HpyCH4III	ACNGT	5 cut(s)	625, 693, 835, 1375, 1688
HpyCH4IV	ACGT	4 cut(s)	204, 270, 422, 1234
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1394, 1447, 1456, 1611
HpyF3I	CTNAG	5 cut(s)	227, 236, 1337, 1398, 1432
HpySE526I	ACGT	4 cut(s)	204, 270, 422, 1234
Hsp92II	CATG	5 cut(s)	53, 1114, 1309, 1388, 1730
HspAI	GCGC	2 cut(s)	11, 1481
Kpn2I	TCCGGA	1 cut(s)	875
KpnI	GGTACC	1 cut(s)	1741
KroI	GCCGGC	1 cut(s)	7
KroNI	GCCGGC	1 cut(s)	9
Ksp22I	TGATCA	1 cut(s)	435
Kzo9I	GATC	8 cut(s)	112, 435, 483, 872, 968, 1516, 1658, 1662
LmnI	GCTCC	3 cut(s)	460, 466, 611
Lsp1109I	GCAGC	2 cut(s)	552, 1406
LweI	GCATC	9 cut(s)	135, 378, 670, 775, 1281, 1323, 1428, 1638, 1711
MaeI	CTAG	5 cut(s)	258, 1269, 1409, 1706, 1762
MaeII	ACGT	4 cut(s)	204, 270, 422, 1234
MaeIII	GTNAC	6 cut(s)	253, 313, 418, 838, 1088, 1468
MalI	GATC	8 cut(s)	114, 437, 485, 874, 970, 1518, 1660, 1664
MboI	GATC	8 cut(s)	112, 435, 483, 872, 968, 1516, 1658, 1662
MboII	GAAGA	5 cut(s)	220, 584, 681, 1130, 1416
MflI	RGATCY	2 cut(s)	872, 968
MhlI	GDGCHC	1 cut(s)	1693
MlsI	TGGCCA	2 cut(s)	998, 1299
MluCI	AATT	8 cut(s)	603, 637, 1076, 1187, 1195, 1227, 1747, 1752
MluNI	TGGCCA	2 cut(s)	998, 1299
MlyI	GAGTC	1 cut(s)	648
MmeI	TCCRAC	1 cut(s)	312
Mox20I	TGGCCA	2 cut(s)	998, 1299
Mph1103I	ATGCAT	1 cut(s)	51
MroI	TCCGGA	1 cut(s)	875
MroNI	GCCGGC	1 cut(s)	7
MscI	TGGCCA	2 cut(s)	998, 1299
MseI	TTAA	8 cut(s)	72, 264, 300, 644, 707, 903, 1698, 1751
MslI	CAYNNNNRTG	1 cut(s)	854
Msp20I	TGGCCA	2 cut(s)	998, 1299
MspI	CCGG	5 cut(s)	8, 190, 725, 876, 1040
MspR9I	CCNGG	3 cut(s)	488, 685, 1289
MvaI	CCWGG	3 cut(s)	488, 685, 1289
MwoI	GCNNNNNNNGC	4 cut(s)	1394, 1447, 1456, 1611
NaeI	GCCGGC	1 cut(s)	9
NdeII	GATC	8 cut(s)	112, 435, 483, 872, 968, 1516, 1658, 1662
NgoMIV	GCCGGC	1 cut(s)	7
NlaIII	CATG	5 cut(s)	53, 1114, 1309, 1388, 1730
NlaIV	GGNNCC	1 cut(s)	1739
NmuCI	GTSAC	4 cut(s)	253, 838, 1088, 1468
NsiI	ATGCAT	1 cut(s)	51
NspI	RCATGY	2 cut(s)	1114, 1730
NspV	TTCGAA	2 cut(s)	89, 184
PaeI	GCATGC	2 cut(s)	1114, 1730
PagI	TCATGA	1 cut(s)	1305
PcsI	WCGNNNNNNNCGW	1 cut(s)	95
PdiI	GCCGGC	1 cut(s)	9
PfeI	GAWTC	4 cut(s)	335, 616, 1381, 1673
PfoI	TCCNGGA	1 cut(s)	486
PinAI	ACCGGT	1 cut(s)	189
PkrI	GCNGC	2 cut(s)	567, 1396
Ple19I	CGATCG	1 cut(s)	1665
PleI	GAGTC	1 cut(s)	648
PpsI	GAGTC	1 cut(s)	648
Ppu21I	YACGTR	1 cut(s)	1235
PshBI	ATTAAT	1 cut(s)	72
PsiI	TTATAA	2 cut(s)	531, 636
Psp6I	CCWGG	3 cut(s)	486, 683, 1287
PspEI	GGTNACC	2 cut(s)	1088, 1468
PspFI	CCCAGC	1 cut(s)	293
PspGI	CCWGG	3 cut(s)	486, 683, 1287
PspN4I	GGNNCC	1 cut(s)	1739
PspPI	GGNCC	1 cut(s)	1042
PsuI	RGATCY	2 cut(s)	872, 968
PvuI	CGATCG	1 cut(s)	1665
RsaI	GTAC	4 cut(s)	1233, 1427, 1681, 1739
RsaNI	GTAC	4 cut(s)	1232, 1426, 1680, 1738
RseI	CAYNNNNRTG	1 cut(s)	854
Rsr2I	CGGWCCG	1 cut(s)	1042
RsrII	CGGWCCG	1 cut(s)	1042
SaqAI	TTAA	8 cut(s)	72, 264, 300, 644, 707, 903, 1698, 1751
SatI	GCNGC	2 cut(s)	566, 1395
Sau3AI	GATC	8 cut(s)	112, 435, 483, 872, 968, 1516, 1658, 1662
Sau96I	GGNCC	1 cut(s)	1042
SchI	GAGTC	1 cut(s)	648
ScrFI	CCNGG	3 cut(s)	488, 685, 1289
SduI	GDGCHC	1 cut(s)	1693
SfaNI	GCATC	9 cut(s)	135, 378, 670, 775, 1281, 1323, 1428, 1638, 1711
SfuI	TTCGAA	2 cut(s)	89, 184
SinI	GGWCC	1 cut(s)	1042
SmiMI	CAYNNNNRTG	1 cut(s)	854
SmlI	CTYRAG	3 cut(s)	149, 587, 1742
SmoI	CTYRAG	3 cut(s)	149, 587, 1742
SphI	GCATGC	2 cut(s)	1114, 1730
Sse9I	AATT	8 cut(s)	603, 637, 1076, 1187, 1195, 1227, 1747, 1752
SsiI	CCGC	3 cut(s)	664, 980, 1538
SspI	AATATT	1 cut(s)	1165
SspMI	CTAG	5 cut(s)	258, 1269, 1409, 1706, 1762
StyD4I	CCNGG	3 cut(s)	486, 683, 1287
StyI	CCWWGG	3 cut(s)	474, 999, 1098
TaaI	ACNGT	5 cut(s)	625, 693, 835, 1375, 1688
TaiI	ACGT	4 cut(s)	207, 273, 425, 1237
TaqII	GACCGA	1 cut(s)	1059
TasI	AATT	8 cut(s)	603, 637, 1076, 1187, 1195, 1227, 1747, 1752
TatI	WGTACW	2 cut(s)	1425, 1679
TfiI	GAWTC	4 cut(s)	335, 616, 1381, 1673
Tru1I	TTAA	8 cut(s)	72, 264, 300, 644, 707, 903, 1698, 1751
Tru9I	TTAA	8 cut(s)	72, 264, 300, 644, 707, 903, 1698, 1751
TscAI	CASTG	1 cut(s)	1693
TseFI	GTSAC	4 cut(s)	253, 838, 1088, 1468
TseI	GCWGC	2 cut(s)	565, 1394
Tsp45I	GTSAC	4 cut(s)	253, 838, 1088, 1468
TspDTI	ATGAA	6 cut(s)	327, 512, 924, 1218, 1322, 1373
TspRI	CASTG	1 cut(s)	1693
VneI	GTGCAC	1 cut(s)	1689
VpaK11BI	GGWCC	1 cut(s)	1042
VspI	ATTAAT	1 cut(s)	72
XapI	RAATTY	1 cut(s)	1747
XbaI	TCTAGA	1 cut(s)	1705
XceI	RCATGY	2 cut(s)	1114, 1730
XmiI	GTMKAC	1 cut(s)	1574
XspI	CTAG	5 cut(s)	258, 1269, 1409, 1706, 1762
Zsp2I	ATGCAT	1 cut(s)	51

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.