MD16G1036000.v1.1

Ubiquitin-2 like Rad60 SUMO-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014096
Physical Location & Seq
Reverse (-)
2597689 .. 2600165
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1036000.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGAGGATTTTTCCGAAGAGCTCGAGCCCTTATTCGATTACAGACGCGTTCAGCCCGTCAATCTAATATGCCTCGACGACGATGAATTAGATGCTCCGTCAGCTTCTCCTCCGAAAAGGAGGAAAATTTCCGATTCCGTGGTTGAGAAGGTTGACGAACCTGTGAAGGTAGTGAATGTTATTAGCTGTGAAGATAAGGAAGAGGAGGACTGGTTACCTCCTCCACCCAAGGTTTCTGCCGGCGCTAAGCGGAAGGGTGGGGATTCTACCATAATGGAACTGAGGAAAAAGAAGCAAGAGCTGGCATCATTTGCACAATCAGCTGAAAATGTGTTGAGATCGGTAGAGGAGTCTGTGAAAAGAGAACTTAGTAGCTCATTGGAGGCTGTTGAAGAACAACCTCCAAAGCCCCGCCCTGAACGAAACAAAATTGTTATATCTATTCAAGACAAAGATGGAGTTAAGCAGTTCCGTATTTATGCGGACGACAAGTTTGAGCGGCTCTTCAAAATGTATGCTGATAATGTTAAGCTTGACCTTCAGAGTCTGGTGTTTTGTTTTGATGGGGACAAAATAGGTCCAGCTGCTACGCCTGATGCCCTTGGGATGGAGGAAAATGACATTATTGAAGTGTTTCTTCAACCGAAGAACTTAATCCGTCACCTTGATGAATCTTTGATGGGGACTGGAAGGGCCTTTGACCGGCATCTTTTGGTGTATGTTTGTAGTTTGTGCAGTACCTGCTCTTTAACTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05418 map03013 map05418
Pfam Domains
Protein Families
Rad60-SLD

Protein Analysis

253

Amino Acids

28.49

Weight (kDa)

4.82

Isoelectric Point (pI)

65.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 144 - 212 8.5e-12 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014096)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68185 AT1G68185 AT1G68185
fragaria_vesca FvH4_4g26560
malus_domestica MD13G1034500.v1.1 MD16G1036000.v1.1
prunus_persica Prupe.1G318000_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0434211
rosa_laevigata RLG00000006692
rosa_multiflora Rmu_sc0000459.1_g000010
rosa_roxburghii Rroxscaffold_5G00375590
rosa_rugosa Rorug04G0275500
rosa_samantha Rh4AG330100 Rh4BG337700 Rh4CG353100 Rh4DG333700
rosa_wichuraiana Rw4G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 749
AccBSI CCGCTC 1 cut(s) 499
AccII CGCG 1 cut(s) 48
AciI CCGC 4 cut(s) 250, 412, 482, 499
AcsI RAATTY 1 cut(s) 126
AcuI CTGAAG 1 cut(s) 524
AfaI GTAC 1 cut(s) 739
AfiI CCNNNNNNNGG 2 cut(s) 607, 702
AflIII ACRYGT 1 cut(s) 46
AgsI TTSAA 5 cut(s) 392, 446, 508, 629, 641
AluBI AGCT 8 cut(s) 22, 104, 186, 301, 323, 375, 532, 584
AluI AGCT 8 cut(s) 22, 104, 186, 301, 323, 375, 532, 584
Alw21I GWGCWC 1 cut(s) 24
AlwNI CAGNNNCTG 2 cut(s) 547, 741
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 1 cut(s) 693
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 1 cut(s) 126
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
Asp700I GAANNNNTTC 1 cut(s) 633
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 2 cut(s) 578, 693
AsuHPI GGTGA 1 cut(s) 653
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 1 cut(s) 578
BanII GRGCYC 2 cut(s) 24, 30
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 1 cut(s) 571
BccI CCATC 4 cut(s) 449, 557, 601, 673
BfoI RGCGCY 1 cut(s) 246
BfuAI ACCTGC 1 cut(s) 749
BisI GCNGC 2 cut(s) 500, 585
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 2 cut(s) 501, 586
Bme18I GGWCC 1 cut(s) 578
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 2 cut(s) 578, 693
BmsI GCATC 4 cut(s) 82, 314, 586, 715
Bpu1102I GCTNAGC 1 cut(s) 246
BsaJI CCNNGG 3 cut(s) 138, 228, 601
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 607, 702
Bse118I RCCGGY 2 cut(s) 239, 702
Bse1I ACTGG 2 cut(s) 215, 691
BseDI CCNNGG 3 cut(s) 138, 228, 601
BseGI GGATG 1 cut(s) 612
BseLI CCNNNNNNNGG 2 cut(s) 607, 702
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 2 cut(s) 215, 691
BseRI GAGGAG 4 cut(s) 99, 210, 218, 362
BseXI GCAGC 1 cut(s) 571
BsgI GTGCAG 1 cut(s) 754
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 1 cut(s) 695
BsiHKAI GWGCWC 1 cut(s) 24
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 2 cut(s) 240, 703
BslFI GGGAC 2 cut(s) 581, 697
BslI CCNNNNNNNGG 2 cut(s) 607, 702
BsmFI GGGAC 2 cut(s) 581, 697
BsnI GGCC 1 cut(s) 695
BsoBI CYCGRG 1 cut(s) 23
Bsp1286I GDGCHC 2 cut(s) 24, 30
Bsp143I GATC 1 cut(s) 338
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 4 cut(s) 250, 412, 482, 499
BspANI GGCC 1 cut(s) 695
BspCNI CTCAG 1 cut(s) 273
BspFNI CGCG 1 cut(s) 48
BspMI ACCTGC 1 cut(s) 749
BspQI GCTCTTC 2 cut(s) 12, 509
BsrBI CCGCTC 1 cut(s) 499
BsrFI RCCGGY 2 cut(s) 239, 702
BsrI ACTGG 2 cut(s) 215, 691
BssAI RCCGGY 2 cut(s) 239, 702
BssECI CCNNGG 3 cut(s) 138, 228, 601
BssMI GATC 1 cut(s) 338
BssT1I CCWWGG 2 cut(s) 228, 601
Bst4CI ACNGT 1 cut(s) 754
Bst6I CTCTTC 3 cut(s) 12, 195, 509
BstAPI GCANNNNNTGC 2 cut(s) 311, 741
BstC8I GCNNGC 2 cut(s) 241, 303
BstDEI CTNAG 3 cut(s) 246, 281, 368
BstDSI CCRYGG 1 cut(s) 138
BstEII GGTNACC 1 cut(s) 213
BstF5I GGATG 1 cut(s) 612
BstFNI CGCG 1 cut(s) 48
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 1 cut(s) 341
BstMBI GATC 1 cut(s) 338
BstMWI GCNNNNNNNGC 4 cut(s) 101, 311, 320, 741
BstPI GGTNACC 1 cut(s) 213
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 571
BsuRI GGCC 1 cut(s) 695
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 1 cut(s) 612
BveI ACCTGC 1 cut(s) 749
Cac8I GCNNGC 2 cut(s) 241, 303
CaiI CAGNNNCTG 2 cut(s) 547, 741
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 2 cut(s) 239, 702
Cfr13I GGNCC 2 cut(s) 578, 693
CseI GACGC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 738
CviQI GTAC 1 cut(s) 738
DdeI CTNAG 3 cut(s) 246, 281, 368
DpnI GATC 1 cut(s) 340
DpnII GATC 1 cut(s) 338
Eam1104I CTCTTC 3 cut(s) 12, 195, 509
EarI CTCTTC 3 cut(s) 12, 195, 509
Ecl136II GAGCTC 1 cut(s) 22
Eco130I CCWWGG 2 cut(s) 228, 601
Eco24I GRGCYC 2 cut(s) 24, 30
Eco47I GGWCC 1 cut(s) 578
Eco53kI GAGCTC 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 524
Eco88I CYCGRG 1 cut(s) 23
Eco91I GGTNACC 1 cut(s) 213
EcoICRI GAGCTC 1 cut(s) 22
EcoO109I RGGNCCY 1 cut(s) 693
EcoO65I GGTNACC 1 cut(s) 213
EcoT14I CCWWGG 2 cut(s) 228, 601
EcoT38I GRGCYC 2 cut(s) 24, 30
ErhI CCWWGG 2 cut(s) 228, 601
FaiI YATR 7 cut(s) 70, 272, 437, 480, 516, 720, 757
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FaqI GGGAC 2 cut(s) 581, 697
FauI CCCGC 1 cut(s) 419
Fnu4HI GCNGC 2 cut(s) 500, 585
FokI GGATG 1 cut(s) 619
FriOI GRGCYC 2 cut(s) 24, 30
Fsp4HI GCNGC 2 cut(s) 500, 585
GlaI GCGC 1 cut(s) 244
GluI GCNGC 2 cut(s) 500, 585
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 1 cut(s) 695
HapII CCGG 2 cut(s) 240, 703
HgaI GACGC 1 cut(s) 54
HhaI GCGC 1 cut(s) 245
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 5 cut(s) 134, 263, 350, 544, 671
HpaII CCGG 2 cut(s) 240, 703
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 4 cut(s) 16, 114, 133, 543
Hpy188III TCNNGA 1 cut(s) 446
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 2 cut(s) 80, 83
HpyAV CCTTC 5 cut(s) 142, 160, 247, 548, 684
HpyCH4III ACNGT 1 cut(s) 754
HpyCH4V TGCA 2 cut(s) 314, 735
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 311, 320, 741
HpyF3I CTNAG 3 cut(s) 246, 281, 368
HspAI GCGC 1 cut(s) 243
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Kzo9I GATC 1 cut(s) 338
LguI GCTCTTC 2 cut(s) 12, 509
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 1 cut(s) 571
LweI GCATC 4 cut(s) 82, 314, 586, 715
MaeIII GTNAC 2 cut(s) 213, 659
MalI GATC 1 cut(s) 340
MbiI CCGCTC 1 cut(s) 499
MboI GATC 1 cut(s) 338
MboII GAAGA 7 cut(s) 29, 203, 212, 404, 496, 629, 658
MhlI GDGCHC 2 cut(s) 24, 30
MluCI AATT 3 cut(s) 86, 126, 429
MluI ACGCGT 1 cut(s) 46
MlyI GAGTC 2 cut(s) 359, 553
MroNI GCCGGC 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 633
MseI TTAA 4 cut(s) 462, 528, 653, 749
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 2 cut(s) 323, 584
MspI CCGG 2 cut(s) 240, 703
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 4 cut(s) 101, 311, 320, 741
NaeI GCCGGC 1 cut(s) 241
NdeII GATC 1 cut(s) 338
NgoMIV GCCGGC 1 cut(s) 239
NmuCI GTSAC 1 cut(s) 659
PaeR7I CTCGAG 1 cut(s) 23
PciSI GCTCTTC 2 cut(s) 12, 509
PdiI GCCGGC 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 3 cut(s) 134, 263, 671
PkrI GCNGC 2 cut(s) 501, 586
PleI GAGTC 2 cut(s) 358, 552
PpsI GAGTC 2 cut(s) 358, 552
Psp124BI GAGCTC 1 cut(s) 24
PspEI GGTNACC 1 cut(s) 213
PspPI GGNCC 2 cut(s) 578, 693
PspXI VCTCGAGB 1 cut(s) 23
PstNI CAGNNNCTG 2 cut(s) 547, 741
PvuII CAGCTG 2 cut(s) 323, 584
RsaI GTAC 1 cut(s) 739
RsaNI GTAC 1 cut(s) 738
RseI CAYNNNNRTG 1 cut(s) 666
SacI GAGCTC 1 cut(s) 24
SapI GCTCTTC 2 cut(s) 12, 509
SaqAI TTAA 4 cut(s) 462, 528, 653, 749
SatI GCNGC 2 cut(s) 500, 585
Sau3AI GATC 1 cut(s) 338
Sau96I GGNCC 2 cut(s) 578, 693
SchI GAGTC 2 cut(s) 359, 553
SduI GDGCHC 2 cut(s) 24, 30
SfaNI GCATC 4 cut(s) 82, 314, 586, 715
Sfr274I CTCGAG 1 cut(s) 23
SinI GGWCC 1 cut(s) 578
SlaI CTCGAG 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 3 cut(s) 86, 126, 429
SsiI CCGC 4 cut(s) 250, 412, 482, 499
SstI GAGCTC 1 cut(s) 24
StyI CCWWGG 2 cut(s) 228, 601
TaaI ACNGT 1 cut(s) 754
TaqI TCGA 3 cut(s) 24, 36, 75
TasI AATT 3 cut(s) 86, 126, 429
TauI GCSGC 1 cut(s) 502
TfiI GAWTC 3 cut(s) 134, 263, 671
Tru1I TTAA 4 cut(s) 462, 528, 653, 749
Tru9I TTAA 4 cut(s) 462, 528, 653, 749
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 2 cut(s) 99, 684
TspGWI ACGGA 4 cut(s) 87, 127, 461, 647
VpaK11BI GGWCC 1 cut(s) 578
XapI RAATTY 1 cut(s) 126
XhoI CTCGAG 1 cut(s) 23
XmnI GAANNNNTTC 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.