MD16G1083100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014338
Physical Location & Seq
Reverse (-)
5816429 .. 5818517
2089 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1083100.v1.1.491

Sequence Viewer

Length: 615 bp
ATGCCTTCAGATTCATCACTCTTTCTCCTACTGCTCTTCTCCATAGCTTTCAATCTGCTCTCTCTAGCCCACGCCACGATCGGAAGCGAAGAAAGCGGAGGAGGGATTGGGAGGAGGGCGCTGCTGAGTCTGAAAGAAACTCCTCACGGAAGCAACGCCACCTACGAATGCTCTCCGGCTGGCCCTTGCGTTCCTTGCCTTTACCCCGAAAAGAAAGACGAGAAGTATCGCTGCAGTGAGACTGGGTATCGCATCCCCTTGAAATGTGTTGAAATGAAACATGGCTCAAAGGAAGCTAAAATGAAAGGTGCTCAAAAGAGTCGATCTGCTCTGGAGATCTCCCACAACATTGAAGAATCGGATAAAGCAGACGACCTTGGTACTTATGTAAAGCATAGAAGTTTACTTGATGATTCATCCACACAAGAGCCACAGGCTTACATTACATATAGAAGCTGTATACCAGCAGTTAATGAAGAGAAGTTGTCGGTGCTTGGTTTCGAGGTGATCGTGCTGTTCTTGTTGCTCATCAGCGGTTCATTAGTATACTTCAGAAGAAAGCAGACTACTTCAACTGCAGGATTTGTACGAATTCAGAACAACTCCAGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

22.55

Weight (kDa)

8.17

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69980
fragaria_vesca FvH4_4g35380 FvH4_4g35380
malus_domestica MD16G1083100.v1.1
prunus_persica Prupe.1G262900_v2.0.a1 Prupe.1G262900_v2.0.a1
pyrus_communis pycom16g07200
rosa_chinensis RchiOBHm_Chr4g0444991
rosa_laevigata RLG00000005779
rosa_multiflora Rmu_ssc0000285.1_g000002
rosa_roxburghii Rroxscaffold_5G00385500
rosa_rugosa Rorug04G0357100 Rorug04G0357200
rosa_samantha Rh4AG419000 Rh4BG430700 Rh4DG426600
rosa_wichuraiana Rw4G035870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 460, 546
AciI CCGC 2 cut(s) 96, 534
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 1 cut(s) 535
AfaI GTAC 2 cut(s) 382, 588
AgsI TTSAA 5 cut(s) 52, 262, 272, 353, 573
AjnI CCWGG 1 cut(s) 605
AluBI AGCT 3 cut(s) 47, 296, 456
AluI AGCT 3 cut(s) 47, 296, 456
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 233
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 2 cut(s) 121, 231
ApoI RAATTY 1 cut(s) 591
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 517
BaeI ACNNNNGTAYC 2 cut(s) 372, 405
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 2 cut(s) 108, 218
BciT130I CCWGG 1 cut(s) 607
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 1 cut(s) 65
BfmI CTRYAG 2 cut(s) 232, 576
BfoI RGCGCY 1 cut(s) 122
BglII AGATCT 1 cut(s) 336
BisI GCNGC 2 cut(s) 122, 232
BlsI GCNGC 2 cut(s) 123, 233
Bme1390I CCNGG 1 cut(s) 607
BmgT120I GGNCC 1 cut(s) 182
BmrFI CCNGG 1 cut(s) 607
BmrI ACTGGG 1 cut(s) 252
BmsI GCATC 1 cut(s) 261
BmuI ACTGGG 1 cut(s) 252
BpmI CTGGAG 2 cut(s) 353, 589
BsaJI CCNNGG 1 cut(s) 376
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bse1I ACTGG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 607
BseDI CCNNGG 1 cut(s) 376
BseGI GGATG 2 cut(s) 252, 416
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 1 cut(s) 247
BseRI GAGGAG 3 cut(s) 114, 127, 132
BseXI GCAGC 2 cut(s) 108, 218
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 1 cut(s) 183
BsiEI CGRYCG 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 233
BsmI GAATGC 1 cut(s) 173
BsnI GGCC 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 4 cut(s) 78, 323, 336, 507
BspACI CCGC 2 cut(s) 96, 534
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 1 cut(s) 117
BspMAI CTGCAG 2 cut(s) 236, 580
BspQI GCTCTTC 1 cut(s) 41
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 1 cut(s) 376
BssMI GATC 4 cut(s) 78, 323, 336, 507
BssNAI GTATAC 2 cut(s) 461, 547
BssT1I CCWWGG 1 cut(s) 376
Bst1107I GTATAC 2 cut(s) 461, 547
Bst2UI CCWGG 1 cut(s) 607
Bst6I CTCTTC 2 cut(s) 41, 471
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 1 cut(s) 125
BstF5I GGATG 2 cut(s) 252, 416
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 4 cut(s) 81, 326, 339, 510
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 4 cut(s) 78, 323, 336, 507
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 2 cut(s) 93, 195
BstNI CCWGG 1 cut(s) 607
BstSCI CCNGG 1 cut(s) 605
BstSFI CTRYAG 2 cut(s) 232, 576
BstV1I GCAGC 2 cut(s) 108, 218
BstX2I RGATCY 1 cut(s) 336
BstYI RGATCY 1 cut(s) 336
BstZ17I GTATAC 2 cut(s) 461, 547
BsuRI GGCC 1 cut(s) 183
BtsCI GGATG 2 cut(s) 252, 416
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 1 cut(s) 241
Cac8I GCNNGC 1 cut(s) 181
CfoI GCGC 1 cut(s) 121
Cfr13I GGNCC 1 cut(s) 182
Csp6I GTAC 2 cut(s) 381, 587
CviAII CATG 1 cut(s) 281
CviJI RGCY 9 cut(s) 47, 68, 179, 183, 285, 296, 430, 437, 456
CviKI_1 RGCY 9 cut(s) 47, 68, 179, 183, 285, 296, 430, 437, 456
CviQI GTAC 2 cut(s) 381, 587
DdeI CTNAG 1 cut(s) 125
DpnI GATC 4 cut(s) 80, 325, 338, 509
DpnII GATC 4 cut(s) 78, 323, 336, 507
Eam1104I CTCTTC 2 cut(s) 41, 471
EarI CTCTTC 2 cut(s) 41, 471
Eco130I CCWWGG 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 535
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 605
EcoT14I CCWWGG 1 cut(s) 376
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 1 cut(s) 284
FaiI YATR 8 cut(s) 44, 282, 387, 396, 448, 450, 461, 547
FatI CATG 1 cut(s) 280
FblI GTMKAC 2 cut(s) 460, 546
Fnu4HI GCNGC 2 cut(s) 122, 232
FokI GGATG 2 cut(s) 239, 403
Fsp4HI GCNGC 2 cut(s) 122, 232
FspBI CTAG 1 cut(s) 65
GlaI GCGC 1 cut(s) 120
GluI GCNGC 2 cut(s) 122, 232
GsuI CTGGAG 2 cut(s) 353, 589
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 1 cut(s) 183
HapII CCGG 1 cut(s) 176
HhaI GCGC 1 cut(s) 121
Hin1II CATG 1 cut(s) 284
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HinfI GANTC 5 cut(s) 11, 127, 319, 356, 413
HpaII CCGG 1 cut(s) 176
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 3 cut(s) 404, 461, 547
Hpy188I TCNGA 6 cut(s) 10, 83, 132, 361, 554, 597
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 3 cut(s) 404, 461, 547
HpyAV CCTTC 1 cut(s) 15
HpyCH4V TGCA 2 cut(s) 234, 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 195
HpyF3I CTNAG 1 cut(s) 125
Hsp92II CATG 1 cut(s) 284
HspAI GCGC 1 cut(s) 119
Kzo9I GATC 4 cut(s) 78, 323, 336, 507
LguI GCTCTTC 1 cut(s) 41
LpnPI CCDG 8 cut(s) 165, 189, 228, 317, 419, 477, 564, 592
Lsp1109I GCAGC 2 cut(s) 108, 218
LweI GCATC 1 cut(s) 261
MaeI CTAG 1 cut(s) 65
MalI GATC 4 cut(s) 80, 325, 338, 509
MboI GATC 4 cut(s) 78, 323, 336, 507
MboII GAAGA 5 cut(s) 28, 101, 365, 488, 567
MflI RGATCY 1 cut(s) 336
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 1 cut(s) 591
MlyI GAGTC 2 cut(s) 136, 328
MnlI CCTC 6 cut(s) 92, 95, 105, 108, 153, 496
MseI TTAA 2 cut(s) 471, 613
MspA1I CMGCKG 1 cut(s) 534
MspI CCGG 1 cut(s) 176
MspR9I CCNGG 1 cut(s) 607
Mva1269I GAATGC 1 cut(s) 173
MvaI CCWGG 1 cut(s) 607
MwoI GCNNNNNNNGC 2 cut(s) 93, 195
NdeII GATC 4 cut(s) 78, 323, 336, 507
NlaIII CATG 1 cut(s) 284
PciSI GCTCTTC 1 cut(s) 41
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 507
PctI GAATGC 1 cut(s) 173
PfeI GAWTC 3 cut(s) 11, 356, 413
PkrI GCNGC 2 cut(s) 123, 233
Ple19I CGATCG 1 cut(s) 81
PleI GAGTC 2 cut(s) 135, 327
PpsI GAGTC 2 cut(s) 135, 327
Psp6I CCWGG 1 cut(s) 605
PspGI CCWGG 1 cut(s) 605
PspPI GGNCC 1 cut(s) 182
PstI CTGCAG 2 cut(s) 236, 580
PsuI RGATCY 1 cut(s) 336
PvuI CGATCG 1 cut(s) 81
RsaI GTAC 2 cut(s) 382, 588
RsaNI GTAC 2 cut(s) 381, 587
SapI GCTCTTC 1 cut(s) 41
SaqAI TTAA 2 cut(s) 471, 613
SatI GCNGC 2 cut(s) 122, 232
Sau3AI GATC 4 cut(s) 78, 323, 336, 507
Sau96I GGNCC 1 cut(s) 182
SchI GAGTC 2 cut(s) 136, 328
ScrFI CCNGG 1 cut(s) 607
SduI GDGCHC 1 cut(s) 313
SetI ASST 8 cut(s) 49, 164, 298, 310, 378, 458, 507, 611
SfaNI GCATC 1 cut(s) 261
SfcI CTRYAG 2 cut(s) 232, 576
Sse9I AATT 1 cut(s) 591
SsiI CCGC 2 cut(s) 96, 534
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 605
StyI CCWWGG 1 cut(s) 376
TaqI TCGA 2 cut(s) 322, 501
TasI AATT 1 cut(s) 591
TfiI GAWTC 3 cut(s) 11, 356, 413
Tru1I TTAA 2 cut(s) 471, 613
Tru9I TTAA 2 cut(s) 471, 613
TscAI CASTG 1 cut(s) 241
TseI GCWGC 2 cut(s) 121, 231
TspDTI ATGAA 5 cut(s) 290, 317, 405, 489, 528
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 1 cut(s) 241
XapI RAATTY 1 cut(s) 591
XmiI GTMKAC 2 cut(s) 460, 546
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.