MD16G1092500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006448
Physical Location & Seq
Reverse (-)
6381843 .. 6384278
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1092500.v1.1.491

Sequence Viewer

Length: 315 bp
ATGGCGGCTAACTGCGCGAGGCGAACCCTACAATTCTCTTCAGCTTCTGCAAAAACCCTACTCTCCCGTCCACCGTCTTCACCTTTCGCCTCCAAAGCTTCCAAGCTAACCGAACTCACTTCCCTCAAGTCCGCTTCTGCTCCTCGACTCGCTGCCCAGAAGCTCAAGTTCTCAAGGCTTCCGGTGGAGTTGGGCGGTGCGCAGTCGTTGATCCCGTTGCACAGTGCTACCGCTTCTGCCTTGTTGACGTCGCTCCTGTCTTTGCACAACACCAGCTGGGGGTGCCTATCAGAAGGTTTCGCTACTCCACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

105

Amino Acids

10.83

Weight (kDa)

10.62

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 251
Acc16I TGCGCA 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 282
AccII CGCG 1 cut(s) 17
AciI CCGC 4 cut(s) 5, 132, 195, 231
AclWI GGATC 1 cut(s) 205
AcuI CTGAAG 1 cut(s) 24
AcyI GRCGYC 1 cut(s) 248
AfiI CCNNNNNNNGG 1 cut(s) 279
AluBI AGCT 5 cut(s) 44, 98, 106, 163, 276
AluI AGCT 5 cut(s) 44, 98, 106, 163, 276
AlwI GGATC 1 cut(s) 205
AlwNI CAGNNNCTG 1 cut(s) 47
ApeKI GCWGC 1 cut(s) 152
AspLEI GCGC 2 cut(s) 17, 202
AsuHPI GGTGA 1 cut(s) 72
BanI GGYRCC 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 139
BfmI CTRYAG 1 cut(s) 311
BisI GCNGC 2 cut(s) 6, 153
BlsI GCNGC 2 cut(s) 7, 154
BmiI GGNNCC 1 cut(s) 284
BpiI GAAGAC 1 cut(s) 69
BpuEI CTTGAG 3 cut(s) 110, 149, 157
BsaHI GRCGYC 1 cut(s) 248
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 279
BseRI GAGGAG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 139
BseYI CCCAGC 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 17
BshNI GGYRCC 1 cut(s) 282
BsiSI CCGG 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 279
Bsp143I GATC 1 cut(s) 210
BspACI CCGC 4 cut(s) 5, 132, 195, 231
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 1 cut(s) 284
BspPI GGATC 1 cut(s) 205
BspT107I GGYRCC 1 cut(s) 282
BssMI GATC 1 cut(s) 210
BssNI GRCGYC 1 cut(s) 248
Bst4CI ACNGT 2 cut(s) 75, 224
Bst6I CTCTTC 1 cut(s) 43
BstACI GRCGYC 1 cut(s) 248
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 2 cut(s) 17, 202
BstKTI GATC 1 cut(s) 213
BstMBI GATC 1 cut(s) 210
BstMWI GCNNNNNNNGC 3 cut(s) 14, 95, 282
BstSFI CTRYAG 1 cut(s) 311
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 229
CaiI CAGNNNCTG 1 cut(s) 47
CfoI GCGC 2 cut(s) 17, 202
CviJI RGCY 7 cut(s) 8, 44, 98, 106, 163, 178, 276
CviKI_1 RGCY 7 cut(s) 8, 44, 98, 106, 163, 178, 276
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 43
EarI CTCTTC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 24
FaiI YATR 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 6, 153
Fsp4HI GCNGC 2 cut(s) 6, 153
FspI TGCGCA 1 cut(s) 201
GlaI GCGC 2 cut(s) 16, 201
GluI GCNGC 2 cut(s) 6, 153
GsaI CCCAGC 1 cut(s) 280
HapII CCGG 1 cut(s) 182
HhaI GCGC 2 cut(s) 17, 202
Hin1I GRCGYC 1 cut(s) 248
Hin6I GCGC 2 cut(s) 15, 200
HinP1I GCGC 2 cut(s) 15, 200
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 1 cut(s) 147
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 72
Hpy166II GTNNAC 2 cut(s) 71, 246
Hpy188I TCNGA 1 cut(s) 292
Hpy8I GTNNAC 2 cut(s) 71, 246
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 2 cut(s) 75, 224
HpyCH4IV ACGT 1 cut(s) 248
HpyCH4V TGCA 3 cut(s) 50, 220, 265
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 95, 282
HpySE526I ACGT 1 cut(s) 248
Hsp92I GRCGYC 1 cut(s) 248
HspAI GCGC 2 cut(s) 15, 200
Kzo9I GATC 1 cut(s) 210
LmnI GCTCC 2 cut(s) 145, 258
LpnPI CCDG 5 cut(s) 170, 195, 262, 269, 286
Lsp1109I GCAGC 1 cut(s) 139
MaeII ACGT 1 cut(s) 248
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MboII GAAGA 2 cut(s) 30, 69
MluCI AATT 1 cut(s) 32
MlyI GAGTC 1 cut(s) 141
MnlI CCTC 4 cut(s) 12, 100, 134, 153
MspA1I CMGCKG 1 cut(s) 276
MspI CCGG 1 cut(s) 182
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 14, 95, 282
NdeII GATC 1 cut(s) 210
NlaIV GGNNCC 1 cut(s) 284
NsbI TGCGCA 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PkrI GCNGC 2 cut(s) 7, 154
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
PspFI CCCAGC 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 284
PstNI CAGNNNCTG 1 cut(s) 47
PvuII CAGCTG 1 cut(s) 276
SatI GCNGC 2 cut(s) 6, 153
Sau3AI GATC 1 cut(s) 210
SchI GAGTC 1 cut(s) 141
SetI ASST 8 cut(s) 46, 85, 100, 108, 165, 251, 278, 298
SfcI CTRYAG 1 cut(s) 311
SmlI CTYRAG 3 cut(s) 125, 164, 172
SmoI CTYRAG 3 cut(s) 125, 164, 172
Sse9I AATT 1 cut(s) 32
SsiI CCGC 4 cut(s) 5, 132, 195, 231
TaaI ACNGT 2 cut(s) 75, 224
TaiI ACGT 1 cut(s) 251
TaqI TCGA 1 cut(s) 145
TasI AATT 1 cut(s) 32
TauI GCSGC 1 cut(s) 8
TscAI CASTG 1 cut(s) 229
TseI GCWGC 1 cut(s) 152
TspRI CASTG 1 cut(s) 229
ZraI GACGTC 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.