MD16G1099500.v1.1

SufE-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015092
Physical Location & Seq
Forward (+)
6929699 .. 6930385
687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1099500.v1.1.491

Sequence Viewer

Length: 687 bp
ATGAACGCTACGGTAATTACAACAACAACACTGCCTCATTTCTCATCCTCCCGCTCCGCCGCCGCCGAATCATCCTCAAACCGCCAATTGCACAACCTTATACCGGCCAAGTTGAAACTCGACAAACAAAACAACAGATGTTCCTTATCCATAAAATGCAGCCCCAGTCGAGGAATTCGAATCACGAGCGCGTTGGCCTTGACGGCGGCGGAGGCACCTCCGTCGAAGGCCACGGAGATGGTGGCTGACAAGATAAAACGGCTCGTTTTGGAATTCAAGTCCCTGGCGGAGCCAATCGACCGCGTGAAGCGGCTACTACACTACGCAGCGAGGCTCCCGCCGGACAATGTGTCGGCGCGGGAGCCAGAGAGCCGAGTCCAGGGGTGCTCGACGCAGGTGTGGGTGGAGGCGGAGATGGACGAGGCGGGGAGGATTAGGTTCCGGGCGGACAGCGACTCGGAGATCTCGAAGGGGTTCTGCTCGTGCCTGATTTGGATGCTCGACGGCGCAGAGGCGGGTGAGGTTCTGGCAGTGGAGACGCATGATTTGCAGGACGTGAATGTGGGGGTGTACGGGAAGGTGAATTCGAGAGTGAACACGTGGCACAACGTGCTGCTGGCGATGCAGAGGAAGGCTCAGGCTTTGGTGACGGAGCGAAAGCAGGGGATACGGGGGACTAATCAGTAA

Protein Analysis

229

Amino Acids

25.08

Weight (kDa)

9.18

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SufE PF02657 91 - 210 1.2e-26 Fe-S metabolism associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67810
fragaria_vesca FvH4_4g25940 FvH4_4g25940
malus_domestica MD13G1098500.v1.1 MD16G1099500.v1.1
prunus_persica Prupe.1G246900_v2.0.a1
pyrus_communis pycom13g08620
rosa_chinensis RchiOBHm_Chr4g0433411
rosa_laevigata RLG00000006755
rosa_multiflora Rmu_sc0000759.1_g000005
rosa_roxburghii Rroxscaffold_5G00374960
rosa_rugosa Rorug04G0268600
rosa_samantha Rh4BG331200 Rh4CG346500 Rh4DG327500
rosa_wichuraiana Rw4G028010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 385
Acc36I ACCTGC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 214
AccBSI CCGCTC 1 cut(s) 54
AccII CGCG 3 cut(s) 191, 303, 358
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 3 cut(s) 174, 272, 583
AcvI CACGTG 1 cut(s) 600
AdeI CACNNNGTG 1 cut(s) 610
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 3 cut(s) 103, 170, 379
AflIII ACRYGT 1 cut(s) 597
AgsI TTSAA 2 cut(s) 115, 277
AhdI GACNNNNNGTC 1 cut(s) 349
AjiI CACGTC 1 cut(s) 556
AjnI CCWGG 2 cut(s) 282, 378
Alw21I GWGCWC 1 cut(s) 389
Alw26I GTCTC 1 cut(s) 530
AoxI GGCC 3 cut(s) 105, 195, 228
ApeKI GCWGC 3 cut(s) 159, 326, 613
ApoI RAATTY 3 cut(s) 174, 272, 583
ArsI GACNNNNNNTTYG 2 cut(s) 529, 561
Asp700I GAANNNNTTC 1 cut(s) 473
AspLEI GCGC 3 cut(s) 191, 358, 509
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 3 cut(s) 530, 592, 658
AsuII TTCGAA 1 cut(s) 178
BanI GGYRCC 1 cut(s) 214
BauI CACGAG 2 cut(s) 184, 481
BbrPI CACGTG 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 389
BbvI GCAGC 3 cut(s) 171, 338, 600
BccI CCATC 2 cut(s) 232, 409
BceAI ACGGC 3 cut(s) 219, 275, 520
BcgI CGANNNNNNTGC 2 cut(s) 204, 238
BciT130I CCWGG 2 cut(s) 284, 380
BciVI GTATCC 1 cut(s) 660
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 1 cut(s) 530
BfuAI ACCTGC 1 cut(s) 385
BfuI GTATCC 1 cut(s) 660
BglI GCCNNNNNGGC 1 cut(s) 203
BglII AGATCT 1 cut(s) 462
BisI GCNGC 7 cut(s) 60, 63, 160, 207, 311, 327, 614
BlsI GCNGC 7 cut(s) 61, 64, 161, 208, 312, 328, 615
Bme1390I CCNGG 3 cut(s) 284, 380, 443
BmeRI GACNNNNNGTC 1 cut(s) 349
BmgBI CACGTC 1 cut(s) 556
BmiI GGNNCC 5 cut(s) 216, 291, 335, 363, 440
BmrFI CCNGG 3 cut(s) 284, 380, 443
BmrI ACTGGG 1 cut(s) 159
BmsI GCATC 2 cut(s) 486, 612
BmuI ACTGGG 1 cut(s) 159
BplI GAGNNNNNCTC 2 cut(s) 619, 651
Bpu10I CCTNAGC 1 cut(s) 636
Bpu14I TTCGAA 1 cut(s) 178
BpuMI CCSGG 1 cut(s) 443
BsaAI YACGTR 1 cut(s) 600
BsaJI CCNNGG 3 cut(s) 231, 282, 379
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 170, 379
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 165
BseBI CCWGG 2 cut(s) 284, 380
BseDI CCNNGG 3 cut(s) 231, 282, 379
BseGI GGATG 3 cut(s) 44, 71, 501
BseLI CCNNNNNNNGG 3 cut(s) 103, 170, 379
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 1 cut(s) 165
BseXI GCAGC 3 cut(s) 171, 338, 600
Bsh1236I CGCG 3 cut(s) 191, 303, 358
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 3 cut(s) 107, 197, 230
BshNI GGYRCC 1 cut(s) 214
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 389
BsiSI CCGG 3 cut(s) 104, 341, 442
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 3 cut(s) 103, 170, 379
BsmAI GTCTC 1 cut(s) 530
BsmBI CGTCTC 1 cut(s) 530
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 3 cut(s) 107, 197, 230
Bsp119I TTCGAA 1 cut(s) 178
Bsp1286I GDGCHC 1 cut(s) 389
Bsp143I GATC 1 cut(s) 462
BspANI GGCC 3 cut(s) 107, 197, 230
BspCNI CTCAG 1 cut(s) 649
BspFNI CGCG 3 cut(s) 191, 303, 358
BspLI GGNNCC 5 cut(s) 216, 291, 335, 363, 440
BspMI ACCTGC 1 cut(s) 385
BspT104I TTCGAA 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 214
BsrBI CCGCTC 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 1 cut(s) 165
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 3 cut(s) 231, 282, 379
BssMI GATC 1 cut(s) 462
BssSI CACGAG 2 cut(s) 184, 481
Bst2BI CACGAG 2 cut(s) 184, 481
Bst2UI CCWGG 2 cut(s) 284, 380
Bst4CI ACNGT 1 cut(s) 13
BstAPI GCANNNNNTGC 2 cut(s) 547, 610
BstBAI YACGTR 1 cut(s) 600
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 1 cut(s) 636
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 3 cut(s) 44, 71, 501
BstFNI CGCG 3 cut(s) 191, 303, 358
BstHHI GCGC 3 cut(s) 191, 358, 509
BstKTI GATC 1 cut(s) 465
BstMAI GTCTC 1 cut(s) 530
BstMBI GATC 1 cut(s) 462
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 5 cut(s) 203, 212, 547, 610, 622
BstNI CCWGG 2 cut(s) 284, 380
BstSCI CCNGG 3 cut(s) 282, 378, 441
BstUI CGCG 3 cut(s) 191, 303, 358
BstV1I GCAGC 3 cut(s) 171, 338, 600
BstX2I RGATCY 1 cut(s) 462
BstXI CCANNNNNNTGG 1 cut(s) 238
BstYI RGATCY 1 cut(s) 462
BsuI GTATCC 1 cut(s) 660
BsuRI GGCC 3 cut(s) 107, 197, 230
BtgI CCRYGG 1 cut(s) 231
BtgZI GCGATG 1 cut(s) 635
BtrI CACGTC 1 cut(s) 556
BtsCI GGATG 3 cut(s) 44, 71, 501
BtsI GCAGTG 2 cut(s) 29, 537
BtsIMutI CAGTG 2 cut(s) 29, 537
BveI ACCTGC 1 cut(s) 385
Cac8I GCNNGC 1 cut(s) 618
CfoI GCGC 3 cut(s) 191, 358, 509
Cfr10I RCCGGY 1 cut(s) 103
CseI GACGC 2 cut(s) 400, 547
Csp6I GTAC 1 cut(s) 571
CviAII CATG 1 cut(s) 542
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 1 cut(s) 636
DpnI GATC 1 cut(s) 464
DpnII GATC 1 cut(s) 462
DraIII CACNNNGTG 1 cut(s) 610
DriI GACNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 105
Eam1105I GACNNNNNGTC 1 cut(s) 349
EciI GGCGGA 5 cut(s) 46, 224, 302, 425, 461
Eco72I CACGTG 1 cut(s) 600
EcoRI GAATTC 3 cut(s) 174, 272, 583
EcoRII CCWGG 2 cut(s) 282, 378
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 1 cut(s) 545
FaiI YATR 3 cut(s) 101, 152, 543
FaqI GGGAC 1 cut(s) 265
FatI CATG 1 cut(s) 541
FauI CCCGC 5 cut(s) 59, 345, 351, 418, 508
Fnu4HI GCNGC 7 cut(s) 60, 63, 160, 207, 311, 327, 614
FokI GGATG 3 cut(s) 31, 58, 508
Fsp4HI GCNGC 7 cut(s) 60, 63, 160, 207, 311, 327, 614
GlaI GCGC 3 cut(s) 190, 357, 508
GluI GCNGC 7 cut(s) 60, 63, 160, 207, 311, 327, 614
HaeIII GGCC 3 cut(s) 107, 197, 230
HapII CCGG 3 cut(s) 104, 341, 442
HgaI GACGC 2 cut(s) 400, 547
HhaI GCGC 3 cut(s) 191, 358, 509
Hin1II CATG 1 cut(s) 545
Hin6I GCGC 3 cut(s) 189, 356, 507
HinP1I GCGC 3 cut(s) 189, 356, 507
HinfI GANTC 4 cut(s) 68, 180, 375, 455
HpaII CCGG 3 cut(s) 104, 341, 442
HphI GGTGA 3 cut(s) 530, 592, 658
Hpy166II GTNNAC 2 cut(s) 571, 595
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 3 cut(s) 184, 466, 588
Hpy8I GTNNAC 2 cut(s) 571, 595
Hpy99I CGWCG 3 cut(s) 226, 394, 506
HpyAV CCTTC 4 cut(s) 220, 463, 571, 625
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4IV ACGT 3 cut(s) 555, 599, 609
HpyCH4V TGCA 4 cut(s) 91, 159, 550, 625
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 212, 547, 610, 622
HpyF3I CTNAG 1 cut(s) 636
HpySE526I ACGT 3 cut(s) 555, 599, 609
Hsp92II CATG 1 cut(s) 545
HspAI GCGC 3 cut(s) 189, 356, 507
Kzo9I GATC 1 cut(s) 462
LmnI GCTCC 5 cut(s) 59, 289, 339, 361, 652
Lsp1109I GCAGC 3 cut(s) 171, 338, 600
LweI GCATC 2 cut(s) 486, 612
MaeII ACGT 3 cut(s) 555, 599, 609
MaeIII GTNAC 1 cut(s) 646
MalI GATC 1 cut(s) 464
MbiI CCGCTC 1 cut(s) 54
MboI GATC 1 cut(s) 462
MfeI CAATTG 1 cut(s) 86
MflI RGATCY 1 cut(s) 462
MhlI GDGCHC 1 cut(s) 389
MluCI AATT 5 cut(s) 15, 86, 174, 272, 583
MlyI GAGTC 2 cut(s) 384, 449
MroXI GAANNNNTTC 1 cut(s) 473
MslI CAYNNNNRTG 1 cut(s) 236
MspI CCGG 3 cut(s) 104, 341, 442
MspR9I CCNGG 3 cut(s) 284, 380, 443
MunI CAATTG 1 cut(s) 86
MvaI CCWGG 2 cut(s) 284, 380
MvnI CGCG 3 cut(s) 191, 303, 358
MwoI GCNNNNNNNGC 5 cut(s) 203, 212, 547, 610, 622
NciI CCSGG 1 cut(s) 443
NdeII GATC 1 cut(s) 462
NlaIII CATG 1 cut(s) 545
NlaIV GGNNCC 5 cut(s) 216, 291, 335, 363, 440
NmeAIII GCCGAG 1 cut(s) 398
NmuCI GTSAC 1 cut(s) 646
NspV TTCGAA 1 cut(s) 178
PaqCI CACCTGC 1 cut(s) 385
PcsI WCGNNNNNNNCGW 2 cut(s) 175, 464
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 2 cut(s) 68, 180
PkrI GCNGC 7 cut(s) 61, 64, 161, 208, 312, 328, 615
PleI GAGTC 2 cut(s) 383, 449
PmaCI CACGTG 1 cut(s) 600
PmlI CACGTG 1 cut(s) 600
PpsI GAGTC 2 cut(s) 383, 449
Ppu21I YACGTR 1 cut(s) 600
Psp6I CCWGG 2 cut(s) 282, 378
PspCI CACGTG 1 cut(s) 600
PspGI CCWGG 2 cut(s) 282, 378
PspN4I GGNNCC 5 cut(s) 216, 291, 335, 363, 440
PsuI RGATCY 1 cut(s) 462
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
RseI CAYNNNNRTG 1 cut(s) 236
SatI GCNGC 7 cut(s) 60, 63, 160, 207, 311, 327, 614
Sau3AI GATC 1 cut(s) 462
SchI GAGTC 2 cut(s) 384, 449
ScrFI CCNGG 3 cut(s) 284, 380, 443
SduI GDGCHC 1 cut(s) 389
SetI ASST 9 cut(s) 99, 220, 399, 440, 525, 558, 582, 602, 612
SfaNI GCATC 2 cut(s) 486, 612
SfuI TTCGAA 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 236
Sse9I AATT 5 cut(s) 15, 86, 174, 272, 583
StyD4I CCNGG 3 cut(s) 282, 378, 441
TaaI ACNGT 1 cut(s) 13
TaiI ACGT 3 cut(s) 558, 602, 612
TaqI TCGA 9 cut(s) 120, 169, 178, 224, 297, 389, 467, 501, 587
TasI AATT 5 cut(s) 15, 86, 174, 272, 583
TauI GCSGC 4 cut(s) 62, 65, 209, 313
TfiI GAWTC 2 cut(s) 68, 180
TscAI CASTG 2 cut(s) 36, 537
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 3 cut(s) 159, 326, 613
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 210, 248, 665
TspRI CASTG 2 cut(s) 36, 537
XapI RAATTY 3 cut(s) 174, 272, 583
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XmnI GAANNNNTTC 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.