Rh4DG327500

SufE-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0015092
Physical Location & Seq
Forward (+)
55131696 .. 55133894
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG327500.1

Sequence Viewer

Length: 678 bp
ATGAACTCATCCACCCCATTAAGACCAACACTCCCCAGTTTCTGTCCCTCTCTTCCCCCCTGCTCCTTCTTCTTCACTCACTCCTCAAACCCTAAATCGCAACGAAATGAGAGGAATCTCGCGTTGAAGTCAATCAAATGCGTCCATAGTGGTTTTGGTTCGTATGCTCCTTGCAAATCAAGCATTCGAAACCCTTCCGCGCTGTCATCTTGCTTGACGGAGGCTCCAGCTCTGGTTCGGACCTCTGACACGGTTGCGGATAAGATGCAACGGCTGGTTTTGGAATTCAAGTCTCTGTCGGAGCCCATAGACCGCGTGAAGAGGCTTTTACACTACGCAGCGAACCTGCCGCCGTGCAGCGAGTCGGCTCGGCAGCCGGAGAGCCGGGTCCCGGGCTGCACGACTCAGGTGTGGCTGGAGGCCGAGCTGGACGAGCTGAGGCGAATGAGGTTCCGAGCCGATAGCGACTCGGAGATCTCGAAAGGGTTCTGCTCGTGCCTGATCTGGATGCTCGACGGCGCCGAGCCGAGGGAGGTCTTGGAAATAAAGGCCAAAGATTTGGAGGATGTGAACGTGGGACTGTATGGAAAGGCCAATTCCAGAGTGAACACGTGGCAAAATGTGTTGTTGGCGATGCAGAGGAAGACTCAGGCTTTGGTTACGGCGGCGGAGAGATAG

Protein Analysis

225

Amino Acids

25.09

Weight (kDa)

8.89

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SufE PF02657 95 - 214 2.6e-29 Fe-S metabolism associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67810
fragaria_vesca FvH4_4g25940 FvH4_4g25940
malus_domestica MD13G1098500.v1.1 MD16G1099500.v1.1
prunus_persica Prupe.1G246900_v2.0.a1
pyrus_communis pycom13g08620
rosa_chinensis RchiOBHm_Chr4g0433411
rosa_laevigata RLG00000006755
rosa_multiflora Rmu_sc0000759.1_g000005
rosa_roxburghii Rroxscaffold_5G00374960
rosa_rugosa Rorug04G0268600
rosa_samantha Rh4BG331200 Rh4CG346500 Rh4DG327500
rosa_wichuraiana Rw4G028010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 354
AccB1I GGYRCC 1 cut(s) 518
AccII CGCG 3 cut(s) 122, 200, 315
AciI CCGC 6 cut(s) 198, 257, 313, 350, 665, 668
AcsI RAATTY 1 cut(s) 284
AcvI CACGTG 1 cut(s) 612
AcyI GRCGYC 1 cut(s) 519
AfiI CCNNNNNNNGG 2 cut(s) 391, 528
AflIII ACRYGT 1 cut(s) 609
AgsI TTSAA 2 cut(s) 127, 289
AluBI AGCT 3 cut(s) 230, 427, 436
AluI AGCT 3 cut(s) 230, 427, 436
Alw26I GTCTC 1 cut(s) 297
AlwNI CAGNNNCTG 1 cut(s) 42
Ama87I CYCGRG 1 cut(s) 391
AoxI GGCC 3 cut(s) 420, 549, 591
ApeKI GCWGC 4 cut(s) 338, 357, 373, 396
ApoI RAATTY 1 cut(s) 284
ArsI GACNNNNNNTTYG 2 cut(s) 637, 669
Asp700I GAANNNNTTC 2 cut(s) 193, 485
AspLEI GCGC 2 cut(s) 202, 521
AspS9I GGNCC 2 cut(s) 240, 388
AsuC2I CCSGG 3 cut(s) 386, 392, 393
AsuII TTCGAA 1 cut(s) 187
AvaI CYCGRG 1 cut(s) 391
AvaII GGWCC 2 cut(s) 240, 388
BanI GGYRCC 1 cut(s) 518
BanII GRGCYC 1 cut(s) 306
BauI CACGAG 1 cut(s) 493
BbrPI CACGTG 1 cut(s) 612
BbsI GAAGAC 1 cut(s) 650
BbvCI CCTCAGC 1 cut(s) 437
BbvI GCAGC 4 cut(s) 350, 369, 383, 385
BceAI ACGGC 3 cut(s) 287, 337, 532
BcnI CCSGG 3 cut(s) 386, 392, 393
BcoDI GTCTC 1 cut(s) 297
BfoI RGCGCY 1 cut(s) 522
BfuAI ACCTGC 1 cut(s) 354
BglII AGATCT 1 cut(s) 474
BisI GCNGC 6 cut(s) 339, 350, 358, 374, 397, 666
BlsI GCNGC 6 cut(s) 340, 351, 359, 375, 398, 667
Bme1390I CCNGG 3 cut(s) 386, 392, 393
Bme18I GGWCC 2 cut(s) 240, 388
BmeT110I CYCGRG 1 cut(s) 391
BmgT120I GGNCC 2 cut(s) 240, 388
BmiI GGNNCC 6 cut(s) 225, 303, 389, 390, 452, 520
BmrFI CCNGG 3 cut(s) 386, 392, 393
BmrI ACTGGG 1 cut(s) 30
BmsI GCATC 3 cut(s) 255, 498, 624
BmuI ACTGGG 1 cut(s) 30
BpiI GAAGAC 1 cut(s) 650
BplI GAGNNNNNCTC 2 cut(s) 631, 663
BpmI CTGGAG 2 cut(s) 210, 437
Bpu10I CCTNAGC 1 cut(s) 437
Bpu14I TTCGAA 1 cut(s) 187
BpuMI CCSGG 3 cut(s) 386, 392, 393
BsaAI YACGTR 1 cut(s) 612
BsaHI GRCGYC 1 cut(s) 519
BsaJI CCNNGG 2 cut(s) 391, 527
BsaXI ACNNNNNCTCC 4 cut(s) 15, 45, 208, 238
Bsc4I CCNNNNNNNGG 2 cut(s) 391, 528
Bse1I ACTGG 1 cut(s) 36
BseDI CCNNGG 2 cut(s) 391, 527
BseGI GGATG 3 cut(s) 8, 513, 571
BseLI CCNNNNNNNGG 2 cut(s) 391, 528
BseMII CTCAG 3 cut(s) 419, 428, 662
BseNI ACTGG 1 cut(s) 36
BseRI GAGGAG 1 cut(s) 73
BseXI GCAGC 4 cut(s) 350, 369, 383, 385
BsgI GTGCAG 2 cut(s) 376, 382
Bsh1236I CGCG 3 cut(s) 122, 200, 315
BshFI GGCC 3 cut(s) 422, 551, 593
BshNI GGYRCC 1 cut(s) 518
BsiHKCI CYCGRG 1 cut(s) 391
BsiSI CCGG 3 cut(s) 377, 385, 392
BslFI GGGAC 3 cut(s) 30, 374, 591
BslI CCNNNNNNNGG 2 cut(s) 391, 528
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 3 cut(s) 30, 374, 591
BsmI GAATGC 1 cut(s) 183
BsnI GGCC 3 cut(s) 422, 551, 593
BsoBI CYCGRG 1 cut(s) 391
Bsp119I TTCGAA 1 cut(s) 187
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 2 cut(s) 474, 501
BspACI CCGC 6 cut(s) 198, 257, 313, 350, 665, 668
BspANI GGCC 3 cut(s) 422, 551, 593
BspCNI CTCAG 3 cut(s) 418, 429, 661
BspFNI CGCG 3 cut(s) 122, 200, 315
BspLI GGNNCC 6 cut(s) 225, 303, 389, 390, 452, 520
BspMI ACCTGC 1 cut(s) 354
BspT104I TTCGAA 1 cut(s) 187
BspT107I GGYRCC 1 cut(s) 518
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 2 cut(s) 391, 527
BssMI GATC 2 cut(s) 474, 501
BssNI GRCGYC 1 cut(s) 519
BssSI CACGAG 1 cut(s) 493
Bst2BI CACGAG 1 cut(s) 493
Bst4CI ACNGT 2 cut(s) 253, 582
Bst6I CTCTTC 2 cut(s) 57, 314
BstACI GRCGYC 1 cut(s) 519
BstBAI YACGTR 1 cut(s) 612
BstBI TTCGAA 1 cut(s) 187
BstDEI CTNAG 3 cut(s) 405, 437, 648
BstF5I GGATG 3 cut(s) 8, 513, 571
BstFNI CGCG 3 cut(s) 122, 200, 315
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 2 cut(s) 202, 521
BstKTI GATC 2 cut(s) 477, 504
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 2 cut(s) 474, 501
BstMWI GCNNNNNNNGC 2 cut(s) 180, 433
BstSCI CCNGG 3 cut(s) 384, 390, 391
BstUI CGCG 3 cut(s) 122, 200, 315
BstV1I GCAGC 4 cut(s) 350, 369, 383, 385
BstV2I GAAGAC 1 cut(s) 650
BstX2I RGATCY 1 cut(s) 474
BstXI CCANNNNNNTGG 1 cut(s) 559
BstYI RGATCY 1 cut(s) 474
BsuRI GGCC 3 cut(s) 422, 551, 593
BtgZI GCGATG 1 cut(s) 647
BtsCI GGATG 3 cut(s) 8, 513, 571
BveI ACCTGC 1 cut(s) 354
CaiI CAGNNNCTG 1 cut(s) 42
CfoI GCGC 2 cut(s) 202, 521
Cfr13I GGNCC 2 cut(s) 240, 388
Cfr9I CCCGGG 1 cut(s) 391
CseI GACGC 1 cut(s) 130
DdeI CTNAG 3 cut(s) 405, 437, 648
DinI GGCGCC 1 cut(s) 520
DpnI GATC 2 cut(s) 476, 503
DpnII GATC 2 cut(s) 474, 501
Eam1104I CTCTTC 2 cut(s) 57, 314
EarI CTCTTC 2 cut(s) 57, 314
Eco24I GRGCYC 1 cut(s) 306
Eco47I GGWCC 2 cut(s) 240, 388
Eco72I CACGTG 1 cut(s) 612
Eco88I CYCGRG 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 388
EcoRI GAATTC 1 cut(s) 284
EcoT38I GRGCYC 1 cut(s) 306
EgeI GGCGCC 1 cut(s) 520
EheI GGCGCC 1 cut(s) 520
FaiI YATR 4 cut(s) 147, 165, 308, 585
FaqI GGGAC 3 cut(s) 30, 374, 591
Fnu4HI GCNGC 6 cut(s) 339, 350, 358, 374, 397, 666
FokI GGATG 2 cut(s) 520, 578
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 6 cut(s) 339, 350, 358, 374, 397, 666
GlaI GCGC 2 cut(s) 201, 520
GluI GCNGC 6 cut(s) 339, 350, 358, 374, 397, 666
GsuI CTGGAG 2 cut(s) 210, 437
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 3 cut(s) 422, 551, 593
HapII CCGG 3 cut(s) 377, 385, 392
HgaI GACGC 1 cut(s) 130
HhaI GCGC 2 cut(s) 202, 521
Hin1I GRCGYC 1 cut(s) 519
Hin6I GCGC 2 cut(s) 200, 519
HinP1I GCGC 2 cut(s) 200, 519
HinfI GANTC 5 cut(s) 115, 362, 403, 467, 646
HpaII CCGG 3 cut(s) 377, 385, 392
Hpy166II GTNNAC 2 cut(s) 571, 607
Hpy188I TCNGA 5 cut(s) 240, 247, 301, 455, 472
Hpy188III TCNNGA 3 cut(s) 478, 505, 600
Hpy8I GTNNAC 2 cut(s) 571, 607
Hpy99I CGWCG 1 cut(s) 518
HpyAV CCTTC 2 cut(s) 76, 204
HpyCH4III ACNGT 2 cut(s) 253, 582
HpyCH4IV ACGT 2 cut(s) 573, 611
HpyCH4V TGCA 5 cut(s) 174, 268, 357, 399, 637
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 433
HpyF3I CTNAG 3 cut(s) 405, 437, 648
HpySE526I ACGT 2 cut(s) 573, 611
Hsp92I GRCGYC 1 cut(s) 519
HspAI GCGC 2 cut(s) 200, 519
KasI GGCGCC 1 cut(s) 518
KflI GGGWCCC 1 cut(s) 388
Kzo9I GATC 2 cut(s) 474, 501
LmnI GCTCC 4 cut(s) 68, 172, 229, 301
Lsp1109I GCAGC 4 cut(s) 350, 369, 383, 385
LweI GCATC 3 cut(s) 255, 498, 624
MaeII ACGT 2 cut(s) 573, 611
MaeIII GTNAC 1 cut(s) 658
MalI GATC 2 cut(s) 476, 503
MboI GATC 2 cut(s) 474, 501
MboII GAAGA 5 cut(s) 44, 61, 64, 331, 655
MflI RGATCY 1 cut(s) 474
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 2 cut(s) 284, 595
Mly113I GGCGCC 1 cut(s) 519
MlyI GAGTC 4 cut(s) 371, 397, 461, 640
MmeI TCCRAC 1 cut(s) 279
MroXI GAANNNNTTC 2 cut(s) 193, 485
MseI TTAA 1 cut(s) 20
MspI CCGG 3 cut(s) 377, 385, 392
MspR9I CCNGG 3 cut(s) 386, 392, 393
Mva1269I GAATGC 1 cut(s) 183
MvnI CGCG 3 cut(s) 122, 200, 315
MwoI GCNNNNNNNGC 2 cut(s) 180, 433
NarI GGCGCC 1 cut(s) 519
NciI CCSGG 3 cut(s) 386, 392, 393
NdeII GATC 2 cut(s) 474, 501
NlaIV GGNNCC 6 cut(s) 225, 303, 389, 390, 452, 520
NmeAIII GCCGAG 4 cut(s) 349, 448, 547, 552
NspV TTCGAA 1 cut(s) 187
PcsI WCGNNNNNNNCGW 2 cut(s) 476, 519
PctI GAATGC 1 cut(s) 183
PdmI GAANNNNTTC 2 cut(s) 193, 485
PfeI GAWTC 1 cut(s) 115
PkrI GCNGC 6 cut(s) 340, 351, 359, 375, 398, 667
PleI GAGTC 4 cut(s) 370, 397, 461, 640
PluTI GGCGCC 1 cut(s) 522
PmaCI CACGTG 1 cut(s) 612
PmlI CACGTG 1 cut(s) 612
PpsI GAGTC 4 cut(s) 370, 397, 461, 640
Ppu21I YACGTR 1 cut(s) 612
PpuMI RGGWCCY 1 cut(s) 388
Psp5II RGGWCCY 1 cut(s) 388
PspCI CACGTG 1 cut(s) 612
PspN4I GGNNCC 6 cut(s) 225, 303, 389, 390, 452, 520
PspPI GGNCC 2 cut(s) 240, 388
PspPPI RGGWCCY 1 cut(s) 388
PstNI CAGNNNCTG 1 cut(s) 42
PsuI RGATCY 1 cut(s) 474
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 6 cut(s) 339, 350, 358, 374, 397, 666
Sau3AI GATC 2 cut(s) 474, 501
Sau96I GGNCC 2 cut(s) 240, 388
SchI GAGTC 4 cut(s) 371, 397, 461, 640
ScrFI CCNGG 3 cut(s) 386, 392, 393
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 3 cut(s) 255, 498, 624
SfoI GGCGCC 1 cut(s) 520
SfuI TTCGAA 1 cut(s) 187
SinI GGWCC 2 cut(s) 240, 388
SmaI CCCGGG 1 cut(s) 393
Sse9I AATT 2 cut(s) 284, 595
SsiI CCGC 6 cut(s) 198, 257, 313, 350, 665, 668
SspDI GGCGCC 1 cut(s) 518
StyD4I CCNGG 3 cut(s) 384, 390, 391
TaaI ACNGT 2 cut(s) 253, 582
TaiI ACGT 2 cut(s) 576, 614
TaqI TCGA 3 cut(s) 187, 479, 513
TasI AATT 2 cut(s) 284, 595
TauI GCSGC 2 cut(s) 352, 668
TfiI GAWTC 1 cut(s) 115
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TseI GCWGC 4 cut(s) 338, 357, 373, 396
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 233
TspMI CCCGGG 1 cut(s) 391
VpaK11BI GGWCC 2 cut(s) 240, 388
XapI RAATTY 1 cut(s) 284
XcmI CCANNNNNNNNNTGG 1 cut(s) 152
XmaI CCCGGG 1 cut(s) 391
XmnI GAANNNNTTC 2 cut(s) 193, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.