Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009585

Physical Location & Seq

Genomic Coordinates Forward (+)

7854630 .. 7860767

Gene Structure 6138 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1111900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2913 bp

ATGGAATCCGATTCCGACTCCGACGGCTCTCACATCTCTGCCACTCCCCCAAGACACACTAAACCTTCATTACCACCACAGCCGCCACCGACAAATCCAAAGCGACAACCGGCGCCAAATCGGAAGCCACCGCCACCAAAGCCAAAGCCTAGCATTCTCTCCTCCCGCACATCGAGAATTAGGTACAGACCTTCGTCTAAGTCCAAACCCATTTCCAAACCCGAAACTCCCTCTTCCGAGCCCACCCGACCCGACCCGCCGCCCGATCCATCTCCTCTCCTCCTCCCCAATTTACCCTACCAAATCTGCCGCTGGCCGTCCGATCCATCCCGCGCGATCCCCACCGTCGATTCCAGCGATACCCTTCTCGCCCCACAGGGCAGGCACTTGTTCCAGTCACCGTCGTTCTCCAAAATCCGACGGACCTCCATCGATTTCGATTCTTTCCAAGACGACGAGCCGCCGCTGTCTTCTTCGGCGCCGAAGCCGGATCCAACGGCTGCGATTGGCGGGTCTGATTCTTCTGCCCCGAATCGGGTACCGCCAGAGCCAAAACGCGAAGCGGCTGCTGCCAAGTCTGGAGTTTCGGCCATAGCTGTGAAGAAACGTTTCAATTTGATTGGAGGCAATGTGCCTTCGGCACCTGTGAAGTTTCGAAGCAGTGGGGCAGAAGGGAACTTTGTGAAGCTCAATCTGAATAGGGGGAAGCGCAAATTCGTGAACCGAAGAGGAAATAGCAGCTCGTCGGGTCGTCGAAAATTTTACAGAAAGGGGAAAAGGAAATTGAAAGCAGTAGGTGAAAATGATGGGGAGACAATTGGTGAAGAAGATGAGTTGGTTGCAGATGCTATTGCAGAACAACAGATTCGAAAGCAAGACAGTAAAAGACCGAAATTTGATTCCGAATCGTTTGAAGAGGCGGTTTCGATGGCTAGAAATGATCCGAGTGATGAGAATTTGTTGAAACTGTTGAGGCTAACTTACGGGTATGAATTGTTCCGAGATGGGCAGTTGGAGGCAATCAAGATGGTGCTTGAGGGGAAGTCGACAATGCTTGTTTTGCCTACGGGTGCTGGCAAATCGTTGTGTTATCAGTTGCCCTCATTGATTTTACCTGGGGTAACTTTGGTTGTCAGTCCATTGATTGCACTGATGATTGACCAGCTTAAGCAGCTGCCTCATATGATTCGTGGCGGTCTTATTTGTAGCAATCAGAGACCTGAAGAAGCTTCCGAGACAATAAGGCTGCTTCAACAAGGGGAGTTAAAGGTGCTTTTTGTTTCGCCGGAGAGGTTTTTGAATGCGGAATTCTTGTCTATATTTTCCGCTAACACAGTTGTATCTCTTGTTGTGGTGGATGAAGCTCACTGTGTTTCAGAATGGTCACATAACTTCCGTCCCTCATATATGAGGCTTAGAGCTTGTTTTCTTCGTGCCAAGCTCAATGTCAACTGCATTCTTGCCATGACAGCTACGGCCACAGCCACAACTTTGGGTTCTGTCATGTCTGCTTTAGAGATTCCTCCAACTAATCTCATTCAAAAAGCAATACTAAGGGATAATTTACAACTTTCAGTGTCATTGAGTGGAAATAGAATGAAAGATCTGCTGATGTTGATCAAGTCAACTTCGTTCAAGGATGTTCAGAGCATCATTATCTACTGCAAATTTCAGTTTGAAACTGAAATGATAAGCAGATACTTATGTGACAATAATATCTCTGCAAAGAGTTACCACAGTGGTATCCCTGCTAAAGATCGTAGTCGAGTGCAGGAGTTGTTTTTCACTAACAAGATACGAGTGGTTGTTGCAACTGTGGCCTTTGGCATGGGCCTTGACAAGAGGGATATTGGTGCTGTGATTCATTACACCTTGCCAGAAAGCTTGGAGGAGTATGTTCAGGAAATTGGACGAGCTGGGCGGGATGGGAGGTTGTCCCATTGCCATCTTTATTGTGATGATATCACATATTTTAAACTTCGCAGTCTTATGTATAGTGATGGTGTGGATGAATATACGGTAAACAAATTCCTCTGTCAAGTATTCACCAGTGATGAGAACTCGCATGGGAAAGTTTGTTCATTGGTTAAAGAAACTGCATCTCGCAAGTTTGATATGAAAGAAGAGGTGATGCTCACACTTCTAACGCAGTTGGAGTTGGGTGAAGTGCAGTATTTGCATTTACTTCCAGAGTTAAATGTGACTTGCACCTTGAATTTTCATAATACATCCCCAGCTTTACTTGCAGGGAAAGATGCTCTTGTTGCTGCAATTTTGAAAAAGTCTGAGACTAAGCAAGGACAATACGTGTTTGACCTACCGACTGTGGCAAATAGCATTGGAGTTACAGCTACTGTCCTTTCCAATCATCTTCAAATTTTAAAGTTGAAAGGAGAAGTAACATACGAACTGAAGGACCAAGCCTACTGTTTTACAATCGTCGAAGCCCCGGCAGATCTTTGTTCTCTATCAGCACATCTCACAAAATGGTTATCAGAGGTTGAAAGTTGCAAGGTGCGGAAGTTGGACGCAATGTTTAATGCGGCAGTTTTTGCTGTTAACTCTTGCGAGAAGACGAAAGGTTGTTGTGATGCCCAACGCACACAATGTTTGCAAAAGAAAATTGTCGAATACTTCGATGGAGATGACAATTCTGAAGTCCCTAATACGATGGGTAAATCCAGCCGATTTTTGCGAGCAGATATAAAAGTCTTTCTGCAGAGCAATTCACAGGCCAAATTCACTCCCAGAGCTGTTGCTAGGATAATGCATGGCATTGCAAGTCCATCCTATCCTTCTACATCCTGGTCTAAAACTCACTTCTGGGGAAGATACACAAGTATAGACTTCCAGGTGGTAATGGACGCGGCAAGGGCGGAACTGGTCAATTTTGTGGGGAAGAATGCTGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009378 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009987 GO:0009991 GO:0010035 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031668 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0034645 GO:0042221 GO:0042623 GO:0042631 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070035 GO:0070887 GO:0071103 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0104004 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

971

Amino Acids

107.51

Weight (kDa)

9.06

Isoelectric Point (pI)

46.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	335 - 495	2.3e-22	DEAD/DEAH box helicase
Helicase_C	PF00271	540 - 643	6.8e-19	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009585)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27880 AT1G27880
fragaria_vesca	FvH4_4g24310 FvH4_4g24310
malus_domestica	MD16G1111900.v1.1
prunus_persica	Prupe.1G233100_v2.0.a1
pyrus_communis	pycom13g09790 pycom16g09490
rosa_chinensis	RchiOBHm_Chr4g0431111
rosa_laevigata	RLG00000006924
rosa_multiflora	Rmu_co7983982.1_g000001 Rmu_co8245775.1_g000001 Rmu_sc0040015.1_g000001
rosa_roxburghii	Rroxscaffold_5G00372700
rosa_rugosa	Rorug04G0248100 Rorug04G0248200 Rorug04G0248300
rosa_samantha	Rh4AG304700 Rh4BG311900 Rh4CG328200 Rh4DG308500
rosa_wichuraiana	Rw4G026480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	538
AccB1I	GGYRCC	4 cut(s)	112, 478, 538, 640
AccI	GTMKAC	1 cut(s)	1046
AccII	CGCG	4 cut(s)	333, 335, 558, 2867
AclI	AACGTT	1 cut(s)	607
AclWI	GGATC	6 cut(s)	260, 317, 331, 485, 498, 935
AcoI	YGGCCR	3 cut(s)	314, 588, 1476
AcuI	CTGAAG	3 cut(s)	1242, 2432, 2676
AcyI	GRCGYC	2 cut(s)	113, 479
AfaI	GTAC	2 cut(s)	185, 540
AfiI	CCNNNNNNNGG	2 cut(s)	534, 535
AflII	CTTAAG	1 cut(s)	1166
AflIII	ACRYGT	1 cut(s)	2307
AjnI	CCWGG	3 cut(s)	1114, 2804, 2850
AloI	GAACNNNNNNTCC	2 cut(s)	2062, 2094
Alw26I	GTCTC	4 cut(s)	806, 1210, 1229, 2282
AlwI	GGATC	6 cut(s)	260, 317, 331, 485, 498, 935
AlwNI	CAGNNNCTG	1 cut(s)	2354
AoxI	GGCC	6 cut(s)	314, 588, 1476, 1818, 1831, 2733
ApeKI	GCWGC	8 cut(s)	500, 566, 569, 738, 1171, 1174, 1246, 2267
Asp700I	GAANNNNTTC	1 cut(s)	608
Asp718I	GGTACC	1 cut(s)	538
AspLEI	GCGC	4 cut(s)	115, 335, 481, 711
AspS9I	GGNCC	3 cut(s)	423, 1831, 2416
AsuC2I	CCSGG	1 cut(s)	2450
AsuHPI	GGTGA	6 cut(s)	390, 809, 833, 2038, 2140, 2174
AsuII	TTCGAA	2 cut(s)	655, 868
AvaII	GGWCC	2 cut(s)	423, 2416
BaeI	ACNNNNGTAYC	8 cut(s)	1323, 1356, 1690, 1723, 1726, 1759, 2824, 2857
BamHI	GGATCC	1 cut(s)	490
BanI	GGYRCC	4 cut(s)	112, 478, 538, 640
BanII	GRGCYC	1 cut(s)	243
BbsI	GAAGAC	2 cut(s)	462, 2579
BbvI	GCAGC	8 cut(s)	487, 553, 556, 750, 1161, 1183, 1233, 2254
BceAI	ACGGC	4 cut(s)	40, 301, 513, 1491
BcgI	CGANNNNNNTGC	2 cut(s)	548, 582
BciT130I	CCWGG	3 cut(s)	1116, 2806, 2852
BciVI	GTATCC	1 cut(s)	1754
BclI	TGATCA	1 cut(s)	1617
BcnI	CCSGG	1 cut(s)	2450
BcoDI	GTCTC	4 cut(s)	806, 1210, 1229, 2282
BfaI	CTAG	3 cut(s)	150, 933, 2760
BfmI	CTRYAG	1 cut(s)	2717
BfoI	RGCGCY	2 cut(s)	116, 482
BfrI	CTTAAG	1 cut(s)	1166
BfuI	GTATCC	1 cut(s)	1754
BglII	AGATCT	2 cut(s)	1603, 2455
Bme1390I	CCNGG	4 cut(s)	1116, 2450, 2806, 2852
Bme18I	GGWCC	2 cut(s)	423, 2416
BmgT120I	GGNCC	3 cut(s)	423, 1831, 2416
BmiI	GGNNCC	5 cut(s)	114, 480, 492, 540, 642
BmrFI	CCNGG	4 cut(s)	1116, 2450, 2806, 2852
BmsI	GCATC	6 cut(s)	835, 1659, 2108, 2121, 2245, 2581
BoxI	GACNNNNGTC	1 cut(s)	193
BpiI	GAAGAC	2 cut(s)	462, 2579
BpmI	CTGGAG	1 cut(s)	600
Bpu14I	TTCGAA	2 cut(s)	655, 868
BpuEI	CTTGAG	1 cut(s)	1055
BpuMI	CCSGG	1 cut(s)	2450
Bsa29I	ATCGAT	1 cut(s)	432
BsaAI	YACGTR	1 cut(s)	2308
BsaBI	GATNNNNATC	1 cut(s)	1616
BsaHI	GRCGYC	2 cut(s)	113, 479
BsaI	GGTCTC	1 cut(s)	1210
BsaJI	CCNNGG	2 cut(s)	1115, 2448
BsaXI	ACNNNNNCTCC	2 cut(s)	615, 645
Bsc4I	CCNNNNNNNGG	2 cut(s)	534, 535
Bse118I	RCCGGY	1 cut(s)	109
Bse1I	ACTGG	3 cut(s)	394, 2049, 2886
Bse3DI	GCAATG	4 cut(s)	634, 1939, 2538, 2775
Bse8I	GATNNNNATC	1 cut(s)	1616
BseBI	CCWGG	3 cut(s)	1116, 2806, 2852
BseCI	ATCGAT	1 cut(s)	432
BseDI	CCNNGG	2 cut(s)	1115, 2448
BseGI	GGATG	8 cut(s)	326, 1363, 1645, 1930, 2014, 2228, 2786, 2801
BseJI	GATNNNNATC	1 cut(s)	1616
BseLI	CCNNNNNNNGG	2 cut(s)	534, 535
BseMI	GCAATG	4 cut(s)	634, 1939, 2538, 2775
BseMII	CTCAG	1 cut(s)	2277
BseNI	ACTGG	3 cut(s)	394, 2049, 2886
BseRI	GAGGAG	5 cut(s)	151, 264, 269, 272, 1904
BseXI	GCAGC	8 cut(s)	487, 553, 556, 750, 1161, 1183, 1233, 2254
BseYI	CCCAGC	2 cut(s)	1916, 2233
BsgI	GTGCAG	2 cut(s)	1790, 2189
Bsh1236I	CGCG	4 cut(s)	333, 335, 558, 2867
BshFI	GGCC	6 cut(s)	316, 590, 1478, 1820, 1833, 2735
BshNI	GGYRCC	4 cut(s)	112, 478, 538, 640
BshVI	ATCGAT	1 cut(s)	432
BsiSI	CCGG	4 cut(s)	110, 488, 1286, 2450
BslFI	GGGAC	3 cut(s)	1383, 1921, 2645
BslI	CCNNNNNNNGG	2 cut(s)	534, 535
BsmAI	GTCTC	4 cut(s)	806, 1210, 1229, 2282
BsmFI	GGGAC	3 cut(s)	1383, 1921, 2645
BsmI	GAATGC	4 cut(s)	153, 1306, 1455, 2908
BsnI	GGCC	6 cut(s)	316, 590, 1478, 1820, 1833, 2735
Bso31I	GGTCTC	1 cut(s)	1210
Bsp119I	TTCGAA	2 cut(s)	655, 868
Bsp1286I	GDGCHC	1 cut(s)	243
Bsp143I	GATC	9 cut(s)	265, 322, 336, 490, 940, 1603, 1617, 1756, 2455
BspANI	GGCC	6 cut(s)	316, 590, 1478, 1820, 1833, 2735
BspCNI	CTCAG	1 cut(s)	2278
BspDI	ATCGAT	1 cut(s)	432
BspFNI	CGCG	4 cut(s)	333, 335, 558, 2867
BspLI	GGNNCC	5 cut(s)	114, 480, 492, 540, 642
BspMAI	CTGCAG	1 cut(s)	2721
BspPI	GGATC	6 cut(s)	260, 317, 331, 485, 498, 935
BspT104I	TTCGAA	2 cut(s)	655, 868
BspT107I	GGYRCC	4 cut(s)	112, 478, 538, 640
BspTI	CTTAAG	1 cut(s)	1166
BspTNI	GGTCTC	1 cut(s)	1210
BsrDI	GCAATG	4 cut(s)	634, 1939, 2538, 2775
BsrFI	RCCGGY	1 cut(s)	109
BsrI	ACTGG	3 cut(s)	394, 2049, 2886
BssAI	RCCGGY	1 cut(s)	109
BssECI	CCNNGG	2 cut(s)	1115, 2448
BssMI	GATC	9 cut(s)	265, 322, 336, 490, 940, 1603, 1617, 1756, 2455
BssNI	GRCGYC	2 cut(s)	113, 479
Bst2UI	CCWGG	3 cut(s)	1116, 2806, 2852
Bst6I	CTCTTC	4 cut(s)	238, 721, 909, 2118
BstACI	GRCGYC	2 cut(s)	113, 479
BstAFI	CTTAAG	1 cut(s)	1166
BstAPI	GCANNNNNTGC	2 cut(s)	2176, 2552
BstBAI	YACGTR	1 cut(s)	2308
BstBI	TTCGAA	2 cut(s)	655, 868
BstC8I	GCNNGC	4 cut(s)	314, 383, 1075, 2697
BstDEI	CTNAG	5 cut(s)	198, 1415, 1553, 2286, 2292
BstF5I	GGATG	8 cut(s)	326, 1363, 1645, 1930, 2014, 2228, 2786, 2801
BstFNI	CGCG	4 cut(s)	333, 335, 558, 2867
BstH2I	RGCGCY	2 cut(s)	116, 482
BstHHI	GCGC	4 cut(s)	115, 335, 481, 711
BstKTI	GATC	9 cut(s)	268, 325, 339, 493, 943, 1606, 1620, 1759, 2458
BstMAI	GTCTC	4 cut(s)	806, 1210, 1229, 2282
BstMBI	GATC	9 cut(s)	265, 322, 336, 490, 940, 1603, 1617, 1756, 2455
BstNI	CCWGG	3 cut(s)	1116, 2806, 2852
BstPAI	GACNNNNGTC	1 cut(s)	193
BstSCI	CCNGG	4 cut(s)	1114, 2448, 2804, 2850
BstSFI	CTRYAG	1 cut(s)	2717
BstUI	CGCG	4 cut(s)	333, 335, 558, 2867
BstV1I	GCAGC	8 cut(s)	487, 553, 556, 750, 1161, 1183, 1233, 2254
BstV2I	GAAGAC	2 cut(s)	462, 2579
BstX2I	RGATCY	3 cut(s)	490, 1603, 2455
BstXI	CCANNNNNNTGG	1 cut(s)	1492
BstYI	RGATCY	3 cut(s)	490, 1603, 2455
Bsu15I	ATCGAT	1 cut(s)	432
BsuI	GTATCC	1 cut(s)	1754
BsuRI	GGCC	6 cut(s)	316, 590, 1478, 1820, 1833, 2735
BsuTUI	ATCGAT	1 cut(s)	432
BtsCI	GGATG	8 cut(s)	326, 1363, 1645, 1930, 2014, 2228, 2786, 2801
BtsI	GCAGTG	1 cut(s)	667
BtsIMutI	CAGTG	6 cut(s)	667, 1148, 1366, 1581, 1744, 2056
Cac8I	GCNNGC	4 cut(s)	314, 383, 1075, 2697
CaiI	CAGNNNCTG	1 cut(s)	2354
CfoI	GCGC	4 cut(s)	115, 335, 481, 711
Cfr10I	RCCGGY	1 cut(s)	109
Cfr13I	GGNCC	3 cut(s)	423, 1831, 2416
ClaI	ATCGAT	1 cut(s)	432
CseI	GACGC	2 cut(s)	2537, 2873
Csp6I	GTAC	2 cut(s)	184, 539
CviAII	CATG	5 cut(s)	1465, 1504, 1828, 2066, 2771
CviQI	GTAC	2 cut(s)	184, 539
DdeI	CTNAG	5 cut(s)	198, 1415, 1553, 2286, 2292
DinI	GGCGCC	2 cut(s)	114, 480
DpnI	GATC	9 cut(s)	267, 324, 338, 492, 942, 1605, 1619, 1758, 2457
DpnII	GATC	9 cut(s)	265, 322, 336, 490, 940, 1603, 1617, 1756, 2455
DraI	TTTAAA	2 cut(s)	1975, 2382
EaeI	YGGCCR	3 cut(s)	314, 588, 1476
Eam1104I	CTCTTC	4 cut(s)	238, 721, 909, 2118
EarI	CTCTTC	4 cut(s)	238, 721, 909, 2118
EciI	GGCGGA	1 cut(s)	2891
Eco24I	GRGCYC	1 cut(s)	243
Eco31I	GGTCTC	1 cut(s)	1210
Eco32I	GATATC	1 cut(s)	1963
Eco47I	GGWCC	2 cut(s)	423, 2416
Eco57I	CTGAAG	3 cut(s)	1242, 2432, 2676
EcoRI	GAATTC	1 cut(s)	1307
EcoRII	CCWGG	3 cut(s)	1114, 2804, 2850
EcoRV	GATATC	1 cut(s)	1963
EcoT22I	ATGCAT	1 cut(s)	2772
EcoT38I	GRGCYC	1 cut(s)	243
EgeI	GGCGCC	2 cut(s)	114, 480
EheI	GGCGCC	2 cut(s)	114, 480
FaeI	CATG	5 cut(s)	1468, 1507, 1831, 2069, 2774
FalI	AAGNNNNNCTT	2 cut(s)	2244, 2276
FaqI	GGGAC	3 cut(s)	1383, 1921, 2645
FatI	CATG	5 cut(s)	1464, 1503, 1827, 2065, 2770
FauI	CCCGC	5 cut(s)	173, 264, 338, 503, 1914
FauNDI	CATATG	1 cut(s)	1182
FbaI	TGATCA	1 cut(s)	1617
FblI	GTMKAC	1 cut(s)	1046
FokI	GGATG	8 cut(s)	313, 1370, 1652, 1937, 2021, 2215, 2773, 2788
FriOI	GRGCYC	1 cut(s)	243
FspBI	CTAG	3 cut(s)	150, 933, 2760
GlaI	GCGC	4 cut(s)	114, 334, 480, 710
GsaI	CCCAGC	2 cut(s)	1920, 2237
GsuI	CTGGAG	1 cut(s)	600
HaeII	RGCGCY	2 cut(s)	116, 482
HaeIII	GGCC	6 cut(s)	316, 590, 1478, 1820, 1833, 2735
HapII	CCGG	4 cut(s)	110, 488, 1286, 2450
HgaI	GACGC	2 cut(s)	2537, 2873
HhaI	GCGC	4 cut(s)	115, 335, 481, 711
Hin1I	GRCGYC	2 cut(s)	113, 479
Hin1II	CATG	5 cut(s)	1468, 1507, 1831, 2069, 2774
Hin6I	GCGC	4 cut(s)	113, 333, 479, 709
HinP1I	GCGC	4 cut(s)	113, 333, 479, 709
HincII	GTYRAC	4 cut(s)	1047, 1450, 1626, 2560
HindII	GTYRAC	4 cut(s)	1047, 1450, 1626, 2560
HindIII	AAGCTT	2 cut(s)	1227, 1882
HpaI	GTTAAC	1 cut(s)	2560
HpaII	CCGG	4 cut(s)	110, 488, 1286, 2450
HphI	GGTGA	6 cut(s)	390, 809, 833, 2038, 2140, 2174
Hpy166II	GTNNAC	6 cut(s)	721, 1047, 1450, 1626, 2023, 2560
Hpy188III	TCNNGA	6 cut(s)	174, 579, 718, 1024, 1901, 2189
Hpy8I	GTNNAC	6 cut(s)	721, 1047, 1450, 1626, 2023, 2560
Hpy99I	CGWCG	8 cut(s)	26, 350, 406, 423, 458, 748, 756, 2444
HpyAV	CCTTC	7 cut(s)	75, 201, 374, 645, 665, 2407, 2805
HpyCH4IV	ACGT	2 cut(s)	607, 2307
HpyF3I	CTNAG	5 cut(s)	198, 1415, 1553, 2286, 2292
HpySE526I	ACGT	2 cut(s)	607, 2307
Hsp92I	GRCGYC	2 cut(s)	113, 479
Hsp92II	CATG	5 cut(s)	1468, 1507, 1831, 2069, 2774
HspAI	GCGC	4 cut(s)	113, 333, 479, 709
KasI	GGCGCC	2 cut(s)	112, 478
KpnI	GGTACC	1 cut(s)	542
Ksp22I	TGATCA	1 cut(s)	1617
KspAI	GTTAAC	1 cut(s)	2560
Kzo9I	GATC	9 cut(s)	265, 322, 336, 490, 940, 1603, 1617, 1756, 2455
Lsp1109I	GCAGC	8 cut(s)	487, 553, 556, 750, 1161, 1183, 1233, 2254
LweI	GCATC	6 cut(s)	835, 1659, 2108, 2121, 2245, 2581
MaeI	CTAG	3 cut(s)	150, 933, 2760
MaeII	ACGT	2 cut(s)	607, 2307
MaeIII	GTNAC	8 cut(s)	396, 1120, 1383, 1706, 1730, 2200, 2344, 2398
MalI	GATC	9 cut(s)	267, 324, 338, 492, 942, 1605, 1619, 1758, 2457
MboI	GATC	9 cut(s)	265, 322, 336, 490, 940, 1603, 1617, 1756, 2455
MfeI	CAATTG	1 cut(s)	816
MflI	RGATCY	3 cut(s)	490, 1603, 2455
MhlI	GDGCHC	1 cut(s)	243
Mly113I	GGCGCC	2 cut(s)	113, 479
MlyI	GAGTC	1 cut(s)	11
MmeI	TCCRAC	8 cut(s)	39, 45, 442, 518, 993, 1550, 2133, 2505
Mph1103I	ATGCAT	1 cut(s)	2772
MroXI	GAANNNNTTC	1 cut(s)	608
MseI	TTAA	8 cut(s)	1167, 1265, 1974, 2088, 2195, 2381, 2538, 2559
MslI	CAYNNNNRTG	1 cut(s)	596
MspA1I	CMGCKG	3 cut(s)	312, 466, 1174
MspCI	CTTAAG	1 cut(s)	1166
MspI	CCGG	4 cut(s)	110, 488, 1286, 2450
MspR9I	CCNGG	4 cut(s)	1116, 2450, 2806, 2852
MunI	CAATTG	1 cut(s)	816
Mva1269I	GAATGC	4 cut(s)	153, 1306, 1455, 2908
MvaI	CCWGG	3 cut(s)	1116, 2806, 2852
MvnI	CGCG	4 cut(s)	333, 335, 558, 2867
NarI	GGCGCC	2 cut(s)	113, 479
NciI	CCSGG	1 cut(s)	2450
NdeI	CATATG	1 cut(s)	1182
NdeII	GATC	9 cut(s)	265, 322, 336, 490, 940, 1603, 1617, 1756, 2455
NlaIII	CATG	5 cut(s)	1468, 1507, 1831, 2069, 2774
NlaIV	GGNNCC	5 cut(s)	114, 480, 492, 540, 642
NmuCI	GTSAC	4 cut(s)	396, 1383, 1706, 2200
NsiI	ATGCAT	1 cut(s)	2772
NspV	TTCGAA	2 cut(s)	655, 868
PcsI	WCGNNNNNNNCGW	2 cut(s)	354, 2634
PctI	GAATGC	4 cut(s)	153, 1306, 1455, 2908
PdmI	GAANNNNTTC	1 cut(s)	608
PleI	GAGTC	1 cut(s)	11
PluTI	GGCGCC	2 cut(s)	116, 482
PpsI	GAGTC	1 cut(s)	11
Ppu21I	YACGTR	1 cut(s)	2308
PshAI	GACNNNNGTC	1 cut(s)	193
Psp1406I	AACGTT	1 cut(s)	607
Psp6I	CCWGG	3 cut(s)	1114, 2804, 2850
PspFI	CCCAGC	2 cut(s)	1916, 2233
PspGI	CCWGG	3 cut(s)	1114, 2804, 2850
PspN4I	GGNNCC	5 cut(s)	114, 480, 492, 540, 642
PspPI	GGNCC	3 cut(s)	423, 1831, 2416
PsrI	GAACNNNNNNTAC	2 cut(s)	980, 1012
PstI	CTGCAG	1 cut(s)	2721
PstNI	CAGNNNCTG	1 cut(s)	2354
PsuI	RGATCY	3 cut(s)	490, 1603, 2455
PvuII	CAGCTG	1 cut(s)	1174
RsaI	GTAC	2 cut(s)	185, 540
RsaNI	GTAC	2 cut(s)	184, 539
RseI	CAYNNNNRTG	1 cut(s)	596
SalI	GTCGAC	1 cut(s)	1045
SaqAI	TTAA	8 cut(s)	1167, 1265, 1974, 2088, 2195, 2381, 2538, 2559
Sau3AI	GATC	9 cut(s)	265, 322, 336, 490, 940, 1603, 1617, 1756, 2455
Sau96I	GGNCC	3 cut(s)	423, 1831, 2416
SchI	GAGTC	1 cut(s)	11
ScrFI	CCNGG	4 cut(s)	1116, 2450, 2806, 2852
SduI	GDGCHC	1 cut(s)	243
SfaNI	GCATC	6 cut(s)	835, 1659, 2108, 2121, 2245, 2581
SfcI	CTRYAG	1 cut(s)	2717
SfoI	GGCGCC	2 cut(s)	114, 480
SfuI	TTCGAA	2 cut(s)	655, 868
SinI	GGWCC	2 cut(s)	423, 2416
SmiMI	CAYNNNNRTG	1 cut(s)	596
SmlI	CTYRAG	2 cut(s)	1034, 1166
SmoI	CTYRAG	2 cut(s)	1034, 1166
SspDI	GGCGCC	2 cut(s)	112, 478
SspMI	CTAG	3 cut(s)	150, 933, 2760
StyD4I	CCNGG	4 cut(s)	1114, 2448, 2804, 2850
TaiI	ACGT	2 cut(s)	610, 2310
TaqII	GACCGA	1 cut(s)	904
TauI	GCSGC	8 cut(s)	85, 262, 312, 463, 466, 566, 2546, 2870
Tru1I	TTAA	8 cut(s)	1167, 1265, 1974, 2088, 2195, 2381, 2538, 2559
Tru9I	TTAA	8 cut(s)	1167, 1265, 1974, 2088, 2195, 2381, 2538, 2559
TscAI	CASTG	6 cut(s)	667, 1155, 1373, 1581, 1744, 2056
TseFI	GTSAC	4 cut(s)	396, 1383, 1706, 2200
TseI	GCWGC	8 cut(s)	500, 566, 569, 738, 1171, 1174, 1246, 2267
Tsp45I	GTSAC	4 cut(s)	396, 1383, 1706, 2200
TspDTI	ATGAA	9 cut(s)	57, 1005, 1374, 1613, 1853, 2025, 2070, 2132, 2210
TspGWI	ACGGA	2 cut(s)	436, 1385
TspRI	CASTG	6 cut(s)	667, 1155, 1373, 1581, 1744, 2056
Vha464I	CTTAAG	1 cut(s)	1166
VpaK11BI	GGWCC	2 cut(s)	423, 2416
XmiI	GTMKAC	1 cut(s)	1046
XmnI	GAANNNNTTC	1 cut(s)	608
XspI	CTAG	3 cut(s)	150, 933, 2760
Zsp2I	ATGCAT	1 cut(s)	2772

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.