Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0009585

Physical Location & Seq

Genomic Coordinates Reverse (-)

52685478 .. 52691635

Gene Structure 6158 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G026480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2796 bp

ATGGAGTCCGATTCAGACTCAGATGGCTCTCACATCTCGGCCACTCCTCCGCGGCCAAAGCCCACCCTTATCTCCAGACCTAGGTCCTCTCAATCCAACTCAAAAGCCAAAAAGCTCTTCTCCGAACCCCCGGCCCCCGCCAGACCCGATCCGTCTCCTCTTCTCTCCTCCGATTTACCTTTCCAGATACTCCGCCGCCCGCCCGGTCAAACCCTCGCGATCCCGACCGTCGATTCCTTCGTCACTCCCGGCTACTTCTCCAAATCGGCGCCGTCGTTCTCCAAAAACCGACGTGTCAGCATCGATTTCGATCCGGACACATACGACCCGCCGTCTACTTTCTCACCTCCGAATCCAAAGCCTCAGCCGGAGGCTGGTATCTCTGGATCTAACTATTCTGCCGCCGATTGGGTACCGCCGCAGCCGGAGCCCAAAGTTGAAGTTGCCAAAGTTGAAGTTGCTAATAGGTCTGGGGTTTCATCCAAAATTGTGAAGAAAAACGTCAATCTGATTCGAGGTAATGCTCCTTTGCCGCCGGTGAAGCTCAAAGGCGGAGCCGAAGGGAACTTTGTGAAGCTCAACCTGAACGGAAGGAAGCGCAAGTTTCTGAACAAGGGCAGAAGAGGAAGTTTCAGTTCTTCAAGTCGTCGGAAATTTTACCGGAAAGGTAAAAGAAAATTGACAGCGCAAGATGGAAATGAAGGAGATGAAGATGGCTTGGTTACAGATGCAATTGCAGAGCAACAGATTCGGAGCCAGGAGGACAGTAAAAGGGCCAAGAAATTCGACCTGGAACCGTTTGAAGAGGCGGTTGCGGCGGCTCGGAGTGAGCAATCCGATGAGAACTTGGTGAAGCTGTTGAGGCTGACTTATGGCTATGATTCGTTCCGAGATGGGCAGCTGGAGGCAATCAAGATGGTGCTGGCCGGAGATTCGACCATGGTTGTTCTGCCGACGGGTGCCGGCAAGTCTTTGTGTTATCAGTTGCCTGCTGTGATTTTACCTGGCGTGACTTTGGTTGTTAGTCCATTGATTGCATTGATGATTGACCAGCTCAAGCAGCTTCCTCATATGATTCGCGGTGGTCTTCTTTGTAGTAGTCAGACACTGGAAGAAGTTTCCGGGACGATTAGGCTGGTTCAAGAAGGGGGCATAAAAGTGCTTTTCGTCTCCCCAGAGAGATTCTTGAATGGAGAATTCCTGTCTATATTTTCTGCCACTCCTATATCTCTTGTTGTGGTGGATGAAGCTCATTGTGTATCTGAATGGTCACACAATTTCCGGCCATCATATATGAGGCTCAGAGCTTCTATGCTTCGTGCCAAGCTCAATGTGAACTGCATTCTTGCAATGACAGCTACTGCCACAGCCACAACTTTGAGTTCTGTCATGTCAGCTTTAGAGATTCCTCCAAACAATCTCATTCAAAAAGCCAACTTAAGGGATAATTTGCAACTATCAGTGTCATCAAGTGGAAATAGAATGAAAGATCTGCTGAAGTTGATCAAGTCTACTTCCTTTAAGGATGTTCAGAGCATCATTATCTACTGCAAATTTCAGTTTGAAACAGAAATGATAAGCAGATACTTATGTGACAACAATATCAATGCAAAGAGTTACCACAGTGGTATCCCTTCAAAAGATCGTACGCGTGTGCAGGAGTTATTTTTTACTAACAAAATTAGAGTGGTTGTTGCAACTGTGGCCTTTGGCATGGGCCTTGACAAGAGGGATATTGGCTCTGTGATTCATTACACCTTACCAGAAAGCTTGGAGGAATATGTTCAGGAAATCGGACGAGCTGGACGGGATGGAAGGTTATCCTATTGCCATCTTTATTTTGATGACAACACATATTACAAACTTCGAAGTCTTATGTATTGTGATGGAGTGGATGAATATGTGGTCAACAAATTCCTCTGCCAAGTATTCACCAGTGATGAGAACTTGAAGGGAAAAATTTATTCTTTGGTTAAAGAAACTGCATCCCGCAAGTTTGATATGAAAGAAGAGGTGATGCTCACAATTCTAACGCAGTTGGAGTTGGGTGAAGTGCAGTATTTGCATTTACTTCCAGAGATAAATGTAACATGCATTTTGAATTTTCATAAGACTTCCCCAGTTTTGCTTGCTGAAAAGGATGTTCTCATTGCAGCAATTCTGAAGAAGTCTGAGACTAAGCACGGACAATGTGTGTTTGACATACCAACTATAGCAAATAGCATTGGAGTTACAACTACCATCCTAACAAACCATTTACAGAATTTAAAGTTGAAAGGAGAAGTAACATATGAAGTGAAGGACCAAGCCTACTGTTATACAATTATGGAGGTCCCTGCAGATCTTTGTTCTCTATCAGCACAGCTTACAAAATGGCTATCAGAGGTTGAAAGTTGTAAGGTGCGGAAGTTGGATGCAATGTTTAATGCGGCAGTCTCTGCTGTTAATAACTGTGAGAAGATGGAAGGTTGTTCTGATGCCCAGCAAACACTATGTTTGCAAAGGAAGATTTTGGAGTATTTCAGTGAAGATAATGTTGATGTCCCTAATAAGATGGGTAAAAGCAGCCCATTTTTGCAAGCAGATATAAAAGTCTTTCTACAGAGCAATTCACAGGCCAAATTCACCCCCAGAGCTGTTGCAAGGATCATGCATGGCATTCCAAGTCCAGCATATGCTTCTGCATTTTGGTCTAAAACTCACTTCTGGGGAAGATATACGCATATAGACTTCCAGGCGGTAATGGAAGCAGCAAAGGTGGAATTGATCAATTTTGTGAGGAAGGATGCAGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009378 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009987 GO:0009991 GO:0010035 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031668 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0034645 GO:0042221 GO:0042623 GO:0042631 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070035 GO:0070887 GO:0071103 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0104004 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

931

Amino Acids

103.51

Weight (kDa)

8.88

Isoelectric Point (pI)

47.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	298 - 459	2.3e-22	DEAD/DEAH box helicase
Helicase_C	PF00271	495 - 605	2.6e-19	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0009585)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27880 AT1G27880
fragaria_vesca	FvH4_4g24310 FvH4_4g24310
malus_domestica	MD16G1111900.v1.1
prunus_persica	Prupe.1G233100_v2.0.a1
pyrus_communis	pycom13g09790 pycom16g09490
rosa_chinensis	RchiOBHm_Chr4g0431111
rosa_laevigata	RLG00000006924
rosa_multiflora	Rmu_co7983982.1_g000001 Rmu_co8245775.1_g000001 Rmu_sc0040015.1_g000001
rosa_roxburghii	Rroxscaffold_5G00372700
rosa_rugosa	Rorug04G0248100 Rorug04G0248200 Rorug04G0248300
rosa_samantha	Rh4AG304700 Rh4BG311900 Rh4CG328200 Rh4DG308500
rosa_wichuraiana	Rw4G026480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	412
AccB1I	GGYRCC	3 cut(s)	268, 412, 959
AccI	GTMKAC	2 cut(s)	335, 1511
AccII	CGCG	4 cut(s)	52, 218, 1080, 1651
AccIII	TCCGGA	1 cut(s)	313
AclWI	GGATC	5 cut(s)	143, 214, 305, 394, 2654
AcoI	YGGCCR	4 cut(s)	39, 53, 924, 1283
AcsI	RAATTY	9 cut(s)	653, 782, 1196, 1553, 1913, 1959, 2101, 2263, 2621
AcuI	CTGAAG	2 cut(s)	1517, 2183
AcyI	GRCGYC	1 cut(s)	269
AfaI	GTAC	2 cut(s)	414, 1648
AfiI	CCNNNNNNNGG	4 cut(s)	81, 374, 408, 1440
AflII	CTTAAG	1 cut(s)	1438
AflIII	ACRYGT	2 cut(s)	292, 1649
AhdI	GACNNNNNGTC	1 cut(s)	331
AjiI	CACGTC	1 cut(s)	293
AjnI	CCWGG	4 cut(s)	756, 789, 1003, 2733
AjuI	GAANNNNNNNTTGG	2 cut(s)	101, 133
Alw26I	GTCTC	4 cut(s)	159, 1174, 2168, 2440
AlwI	GGATC	5 cut(s)	143, 214, 305, 394, 2654
AlwNI	CAGNNNCTG	3 cut(s)	1108, 1361, 2438
Aor13HI	TCCGGA	1 cut(s)	313
AoxI	GGCC	9 cut(s)	39, 53, 132, 774, 924, 1283, 1704, 1717, 2616
ApeKI	GCWGC	6 cut(s)	421, 898, 1060, 2153, 2565, 2750
ApoI	RAATTY	9 cut(s)	653, 782, 1196, 1553, 1913, 1959, 2101, 2263, 2621
Asp700I	GAANNNNTTC	1 cut(s)	1782
Asp718I	GGTACC	1 cut(s)	412
AspA2I	CCTAGG	1 cut(s)	80
AspLEI	GCGC	3 cut(s)	271, 600, 688
AspS9I	GGNCC	6 cut(s)	84, 133, 774, 1717, 2302, 2332
AsuC2I	CCSGG	4 cut(s)	131, 204, 249, 1123
AsuHPI	GGTGA	7 cut(s)	336, 550, 862, 1924, 2026, 2060, 2617
AsuII	TTCGAA	1 cut(s)	1867
AvaII	GGWCC	3 cut(s)	84, 2302, 2332
AvrII	CCTAGG	1 cut(s)	80
BaeI	ACNNNNGTAYC	4 cut(s)	1576, 1609, 1612, 1645
BanI	GGYRCC	3 cut(s)	268, 412, 959
BanII	GRGCYC	1 cut(s)	432
BbsI	GAAGAC	1 cut(s)	1079
BbvCI	CCTCAGC	1 cut(s)	363
BbvI	GCAGC	6 cut(s)	433, 910, 1072, 2165, 2577, 2762
BceAI	ACGGC	2 cut(s)	256, 316
BcgI	CGANNNNNNTGC	4 cut(s)	289, 323, 731, 765
BciT130I	CCWGG	4 cut(s)	758, 791, 1005, 2735
BciVI	GTATCC	1 cut(s)	1640
BclI	TGATCA	2 cut(s)	1503, 2766
BcnI	CCSGG	4 cut(s)	131, 204, 249, 1123
BcoDI	GTCTC	4 cut(s)	159, 1174, 2168, 2440
BfaI	CTAG	2 cut(s)	81, 2794
BfmI	CTRYAG	3 cut(s)	2211, 2337, 2600
BfoI	RGCGCY	1 cut(s)	272
BfrI	CTTAAG	1 cut(s)	1438
BfuI	GTATCC	1 cut(s)	1640
BglII	AGATCT	2 cut(s)	1489, 2341
BlnI	CCTAGG	1 cut(s)	80
Bme1390I	CCNGG	8 cut(s)	131, 204, 249, 758, 791, 1005, 1123, 2735
Bme18I	GGWCC	3 cut(s)	84, 2302, 2332
BmeRI	GACNNNNNGTC	1 cut(s)	331
BmgBI	CACGTC	1 cut(s)	293
BmgT120I	GGNCC	6 cut(s)	84, 133, 774, 1717, 2302, 2332
BmiI	GGNNCC	9 cut(s)	135, 270, 414, 429, 556, 755, 795, 961, 2334
BmrFI	CCNGG	8 cut(s)	131, 204, 249, 758, 791, 1005, 1123, 2735
BmrI	ACTGGG	1 cut(s)	2114
BmsI	GCATC	8 cut(s)	309, 718, 1545, 1994, 2007, 2404, 2467, 2776
BmuI	ACTGGG	1 cut(s)	2114
BoxI	GACNNNNGTC	1 cut(s)	82
BpiI	GAAGAC	1 cut(s)	1079
BpmI	CTGGAG	2 cut(s)	58, 923
Bpu10I	CCTNAGC	1 cut(s)	363
Bpu14I	TTCGAA	1 cut(s)	1867
BpuEI	CTTGAG	1 cut(s)	1040
BpuMI	CCSGG	4 cut(s)	131, 204, 249, 1123
Bsa29I	ATCGAT	1 cut(s)	303
BsaBI	GATNNNNATC	1 cut(s)	309
BsaHI	GRCGYC	1 cut(s)	269
BsaJI	CCNNGG	4 cut(s)	50, 80, 129, 939
BsaWI	WCCGGW	2 cut(s)	313, 660
Bsc4I	CCNNNNNNNGG	4 cut(s)	81, 374, 408, 1440
Bse118I	RCCGGY	2 cut(s)	535, 962
Bse1I	ACTGG	3 cut(s)	1113, 1935, 2120
Bse3DI	GCAATG	3 cut(s)	1356, 2148, 2424
Bse8I	GATNNNNATC	1 cut(s)	309
BseAI	TCCGGA	1 cut(s)	313
BseBI	CCWGG	4 cut(s)	758, 791, 1005, 2735
BseCI	ATCGAT	1 cut(s)	303
BseDI	CCNNGG	4 cut(s)	50, 80, 129, 939
BseJI	GATNNNNATC	1 cut(s)	309
BseLI	CCNNNNNNNGG	4 cut(s)	81, 374, 408, 1440
BseMI	GCAATG	3 cut(s)	1356, 2148, 2424
BseMII	CTCAG	4 cut(s)	33, 377, 1315, 2163
BseNI	ACTGG	3 cut(s)	1113, 1935, 2120
BseRI	GAGGAG	3 cut(s)	36, 147, 157
BseXI	GCAGC	6 cut(s)	433, 910, 1072, 2165, 2577, 2762
BseYI	CCCAGC	1 cut(s)	2481
BsgI	GTGCAG	2 cut(s)	1676, 2075
Bsh1236I	CGCG	4 cut(s)	52, 218, 1080, 1651
Bsh1285I	CGRYCG	1 cut(s)	228
BshFI	GGCC	9 cut(s)	41, 55, 134, 776, 926, 1285, 1706, 1719, 2618
BshNI	GGYRCC	3 cut(s)	268, 412, 959
BshVI	ATCGAT	1 cut(s)	303
BsiEI	CGRYCG	1 cut(s)	228
BsiWI	CGTACG	1 cut(s)	1646
BslFI	GGGAC	3 cut(s)	1138, 2318, 2528
BslI	CCNNNNNNNGG	4 cut(s)	81, 374, 408, 1440
BsmAI	GTCTC	4 cut(s)	159, 1174, 2168, 2440
BsmBI	CGTCTC	2 cut(s)	159, 1174
BsmFI	GGGAC	3 cut(s)	1138, 2318, 2528
BsmI	GAATGC	2 cut(s)	1341, 2658
BsnI	GGCC	9 cut(s)	41, 55, 134, 776, 926, 1285, 1706, 1719, 2618
Bsp119I	TTCGAA	1 cut(s)	1867
Bsp1286I	GDGCHC	1 cut(s)	432
Bsp13I	TCCGGA	1 cut(s)	313
Bsp19I	CCATGG	1 cut(s)	939
Bsp68I	TCGCGA	1 cut(s)	218
BspANI	GGCC	9 cut(s)	41, 55, 134, 776, 926, 1285, 1706, 1719, 2618
BspCNI	CTCAG	4 cut(s)	32, 376, 1314, 2164
BspDI	ATCGAT	1 cut(s)	303
BspEI	TCCGGA	1 cut(s)	313
BspFNI	CGCG	4 cut(s)	52, 218, 1080, 1651
BspLI	GGNNCC	9 cut(s)	135, 270, 414, 429, 556, 755, 795, 961, 2334
BspMAI	CTGCAG	1 cut(s)	2341
BspPI	GGATC	5 cut(s)	143, 214, 305, 394, 2654
BspQI	GCTCTTC	1 cut(s)	122
BspT104I	TTCGAA	1 cut(s)	1867
BspT107I	GGYRCC	3 cut(s)	268, 412, 959
BspTI	CTTAAG	1 cut(s)	1438
BsrDI	GCAATG	3 cut(s)	1356, 2148, 2424
BsrFI	RCCGGY	2 cut(s)	535, 962
BsrI	ACTGG	3 cut(s)	1113, 1935, 2120
BssAI	RCCGGY	2 cut(s)	535, 962
BssECI	CCNNGG	4 cut(s)	50, 80, 129, 939
BssNI	GRCGYC	1 cut(s)	269
BssT1I	CCWWGG	2 cut(s)	80, 939
Bst2UI	CCWGG	4 cut(s)	758, 791, 1005, 2735
Bst4CI	ACNGT	7 cut(s)	229, 767, 798, 1625, 1702, 2315, 2453
Bst6I	CTCTTC	5 cut(s)	122, 165, 616, 798, 2004
BstACI	GRCGYC	1 cut(s)	269
BstAFI	CTTAAG	1 cut(s)	1438
BstAPI	GCANNNNNTGC	2 cut(s)	2062, 2438
BstBI	TTCGAA	1 cut(s)	1867
BstC8I	GCNNGC	6 cut(s)	200, 924, 964, 990, 2130, 2580
BstDEI	CTNAG	5 cut(s)	19, 363, 1301, 2172, 2178
BstDSI	CCRYGG	2 cut(s)	50, 939
BstFNI	CGCG	4 cut(s)	52, 218, 1080, 1651
BstH2I	RGCGCY	1 cut(s)	272
BstHHI	GCGC	3 cut(s)	271, 600, 688
BstMAI	GTCTC	4 cut(s)	159, 1174, 2168, 2440
BstMCI	CGRYCG	1 cut(s)	228
BstNI	CCWGG	4 cut(s)	758, 791, 1005, 2735
BstNSI	RCATGY	1 cut(s)	2094
BstPAI	GACNNNNGTC	1 cut(s)	82
BstSCI	CCNGG	8 cut(s)	129, 202, 247, 756, 789, 1003, 1121, 2733
BstSFI	CTRYAG	3 cut(s)	2211, 2337, 2600
BstUI	CGCG	4 cut(s)	52, 218, 1080, 1651
BstV1I	GCAGC	6 cut(s)	433, 910, 1072, 2165, 2577, 2762
BstV2I	GAAGAC	1 cut(s)	1079
BstX2I	RGATCY	3 cut(s)	386, 1489, 2341
BstYI	RGATCY	3 cut(s)	386, 1489, 2341
Bsu15I	ATCGAT	1 cut(s)	303
BsuI	GTATCC	1 cut(s)	1640
BsuRI	GGCC	9 cut(s)	41, 55, 134, 776, 926, 1285, 1706, 1719, 2618
BsuTUI	ATCGAT	1 cut(s)	303
BtgI	CCRYGG	2 cut(s)	50, 939
BtrI	CACGTC	1 cut(s)	293
BtsIMutI	CAGTG	5 cut(s)	1106, 1467, 1630, 1942, 2530
BtuMI	TCGCGA	1 cut(s)	218
Cac8I	GCNNGC	6 cut(s)	200, 924, 964, 990, 2130, 2580
CaiI	CAGNNNCTG	3 cut(s)	1108, 1361, 2438
CfoI	GCGC	3 cut(s)	271, 600, 688
Cfr10I	RCCGGY	2 cut(s)	535, 962
Cfr13I	GGNCC	6 cut(s)	84, 133, 774, 1717, 2302, 2332
Cfr42I	CCGCGG	1 cut(s)	53
ClaI	ATCGAT	1 cut(s)	303
Csp6I	GTAC	2 cut(s)	413, 1647
CviAII	CATG	6 cut(s)	940, 1390, 1714, 2091, 2650, 2654
CviQI	GTAC	2 cut(s)	413, 1647
DdeI	CTNAG	5 cut(s)	19, 363, 1301, 2172, 2178
DinI	GGCGCC	1 cut(s)	270
DraI	TTTAAA	1 cut(s)	2268
DriI	GACNNNNNGTC	1 cut(s)	331
EaeI	YGGCCR	4 cut(s)	39, 53, 924, 1283
Eam1104I	CTCTTC	5 cut(s)	122, 165, 616, 798, 2004
Eam1105I	GACNNNNNGTC	1 cut(s)	331
EarI	CTCTTC	5 cut(s)	122, 165, 616, 798, 2004
EciI	GGCGGA	2 cut(s)	182, 567
Eco130I	CCWWGG	2 cut(s)	80, 939
Eco24I	GRGCYC	1 cut(s)	432
Eco47I	GGWCC	3 cut(s)	84, 2302, 2332
Eco57I	CTGAAG	2 cut(s)	1517, 2183
EcoO109I	RGGNCCY	2 cut(s)	84, 2332
EcoRI	GAATTC	1 cut(s)	1196
EcoRII	CCWGG	4 cut(s)	756, 789, 1003, 2733
EcoT14I	CCWWGG	2 cut(s)	80, 939
EcoT22I	ATGCAT	2 cut(s)	2096, 2655
EcoT38I	GRGCYC	1 cut(s)	432
EgeI	GGCGCC	1 cut(s)	270
EheI	GGCGCC	1 cut(s)	270
ErhI	CCWWGG	2 cut(s)	80, 939
Esp3I	CGTCTC	2 cut(s)	159, 1174
FaeI	CATG	6 cut(s)	943, 1393, 1717, 2094, 2653, 2657
FaqI	GGGAC	3 cut(s)	1138, 2318, 2528
FatI	CATG	6 cut(s)	939, 1389, 1713, 2090, 2649, 2653
FauI	CCCGC	4 cut(s)	145, 207, 336, 1997
FauNDI	CATATG	3 cut(s)	1071, 2290, 2674
FbaI	TGATCA	2 cut(s)	1503, 2766
FblI	GTMKAC	2 cut(s)	335, 1511
FokI	GGATG	9 cut(s)	466, 1256, 1538, 1823, 1907, 1972, 2153, 2228, 2426
FriOI	GRGCYC	1 cut(s)	432
FspBI	CTAG	2 cut(s)	81, 2794
GlaI	GCGC	3 cut(s)	270, 599, 687
GsaI	CCCAGC	1 cut(s)	2485
GsuI	CTGGAG	2 cut(s)	58, 923
HaeII	RGCGCY	1 cut(s)	272
HaeIII	GGCC	9 cut(s)	41, 55, 134, 776, 926, 1285, 1706, 1719, 2618
HhaI	GCGC	3 cut(s)	271, 600, 688
Hin1I	GRCGYC	1 cut(s)	269
Hin1II	CATG	6 cut(s)	943, 1393, 1717, 2094, 2653, 2657
Hin6I	GCGC	3 cut(s)	269, 598, 686
HinP1I	GCGC	3 cut(s)	269, 598, 686
HincII	GTYRAC	1 cut(s)	1909
HindII	GTYRAC	1 cut(s)	1909
HindIII	AAGCTT	1 cut(s)	1768
HphI	GGTGA	7 cut(s)	336, 550, 862, 1924, 2026, 2060, 2617
Hpy166II	GTNNAC	4 cut(s)	336, 1336, 1512, 1909
Hpy8I	GTNNAC	4 cut(s)	336, 1336, 1512, 1909
Hpy99I	CGWCG	5 cut(s)	233, 277, 294, 651, 958
HpyCH4III	ACNGT	7 cut(s)	229, 767, 798, 1625, 1702, 2315, 2453
HpyCH4IV	ACGT	2 cut(s)	292, 501
HpyF3I	CTNAG	5 cut(s)	19, 363, 1301, 2172, 2178
HpySE526I	ACGT	2 cut(s)	292, 501
Hsp92I	GRCGYC	1 cut(s)	269
Hsp92II	CATG	6 cut(s)	943, 1393, 1717, 2094, 2653, 2657
HspAI	GCGC	3 cut(s)	269, 598, 686
KasI	GGCGCC	1 cut(s)	268
Kpn2I	TCCGGA	1 cut(s)	313
KpnI	GGTACC	1 cut(s)	416
KroI	GCCGGC	1 cut(s)	962
KroNI	GCCGGC	1 cut(s)	964
Ksp22I	TGATCA	2 cut(s)	1503, 2766
KspI	CCGCGG	1 cut(s)	53
LguI	GCTCTTC	1 cut(s)	122
LmnI	GCTCC	4 cut(s)	427, 529, 554, 753
Lsp1109I	GCAGC	6 cut(s)	433, 910, 1072, 2165, 2577, 2762
LweI	GCATC	8 cut(s)	309, 718, 1545, 1994, 2007, 2404, 2467, 2776
MaeI	CTAG	2 cut(s)	81, 2794
MaeII	ACGT	2 cut(s)	292, 501
MaeIII	GTNAC	9 cut(s)	241, 721, 1009, 1269, 1592, 1616, 2086, 2230, 2284
MfeI	CAATTG	1 cut(s)	732
MflI	RGATCY	3 cut(s)	386, 1489, 2341
MhlI	GDGCHC	1 cut(s)	432
MluI	ACGCGT	1 cut(s)	1649
Mly113I	GGCGCC	1 cut(s)	269
MlyI	GAGTC	2 cut(s)	11, 14
MmeI	TCCRAC	4 cut(s)	120, 629, 2019, 2391
Mph1103I	ATGCAT	2 cut(s)	2096, 2655
MroI	TCCGGA	1 cut(s)	313
MroNI	GCCGGC	1 cut(s)	962
MroXI	GAANNNNTTC	1 cut(s)	1782
MseI	TTAA	6 cut(s)	1439, 1521, 1974, 2267, 2424, 2445
MslI	CAYNNNNRTG	1 cut(s)	1157
MspA1I	CMGCKG	2 cut(s)	52, 901
MspCI	CTTAAG	1 cut(s)	1438
MspR9I	CCNGG	8 cut(s)	131, 204, 249, 758, 791, 1005, 1123, 2735
MunI	CAATTG	1 cut(s)	732
Mva1269I	GAATGC	2 cut(s)	1341, 2658
MvaI	CCWGG	4 cut(s)	758, 791, 1005, 2735
MvnI	CGCG	4 cut(s)	52, 218, 1080, 1651
NaeI	GCCGGC	1 cut(s)	964
NarI	GGCGCC	1 cut(s)	269
NciI	CCSGG	4 cut(s)	131, 204, 249, 1123
NcoI	CCATGG	1 cut(s)	939
NdeI	CATATG	3 cut(s)	1071, 2290, 2674
NgoMIV	GCCGGC	1 cut(s)	962
NlaIII	CATG	6 cut(s)	943, 1393, 1717, 2094, 2653, 2657
NlaIV	GGNNCC	9 cut(s)	135, 270, 414, 429, 556, 755, 795, 961, 2334
NmeAIII	GCCGAG	1 cut(s)	17
NmuCI	GTSAC	4 cut(s)	241, 1009, 1269, 1592
NruI	TCGCGA	1 cut(s)	218
NsiI	ATGCAT	2 cut(s)	2096, 2655
NspI	RCATGY	1 cut(s)	2094
NspV	TTCGAA	1 cut(s)	1867
PciSI	GCTCTTC	1 cut(s)	122
PcsI	WCGNNNNNNNCGW	2 cut(s)	237, 272
PctI	GAATGC	2 cut(s)	1341, 2658
PdiI	GCCGGC	1 cut(s)	964
PdmI	GAANNNNTTC	1 cut(s)	1782
Pfl23II	CGTACG	1 cut(s)	1646
PfoI	TCCNGGA	1 cut(s)	1121
PleI	GAGTC	2 cut(s)	11, 13
PluTI	GGCGCC	1 cut(s)	272
PpsI	GAGTC	2 cut(s)	11, 13
PpuMI	RGGWCCY	2 cut(s)	84, 2332
PshAI	GACNNNNGTC	1 cut(s)	82
Psp5II	RGGWCCY	2 cut(s)	84, 2332
Psp6I	CCWGG	4 cut(s)	756, 789, 1003, 2733
PspFI	CCCAGC	1 cut(s)	2481
PspGI	CCWGG	4 cut(s)	756, 789, 1003, 2733
PspLI	CGTACG	1 cut(s)	1646
PspN4I	GGNNCC	9 cut(s)	135, 270, 414, 429, 556, 755, 795, 961, 2334
PspPI	GGNCC	6 cut(s)	84, 133, 774, 1717, 2302, 2332
PspPPI	RGGWCCY	2 cut(s)	84, 2332
PstI	CTGCAG	1 cut(s)	2341
PstNI	CAGNNNCTG	3 cut(s)	1108, 1361, 2438
PsuI	RGATCY	3 cut(s)	386, 1489, 2341
PvuII	CAGCTG	1 cut(s)	901
RruI	TCGCGA	1 cut(s)	218
RsaI	GTAC	2 cut(s)	414, 1648
RsaNI	GTAC	2 cut(s)	413, 1647
RseI	CAYNNNNRTG	1 cut(s)	1157
SacII	CCGCGG	1 cut(s)	53
SapI	GCTCTTC	1 cut(s)	122
SaqAI	TTAA	6 cut(s)	1439, 1521, 1974, 2267, 2424, 2445
Sau96I	GGNCC	6 cut(s)	84, 133, 774, 1717, 2302, 2332
SchI	GAGTC	2 cut(s)	11, 14
ScrFI	CCNGG	8 cut(s)	131, 204, 249, 758, 791, 1005, 1123, 2735
SduI	GDGCHC	1 cut(s)	432
SfaNI	GCATC	8 cut(s)	309, 718, 1545, 1994, 2007, 2404, 2467, 2776
SfcI	CTRYAG	3 cut(s)	2211, 2337, 2600
SfoI	GGCGCC	1 cut(s)	270
Sfr303I	CCGCGG	1 cut(s)	53
SfuI	TTCGAA	1 cut(s)	1867
SgrAI	CRCCGGYG	1 cut(s)	535
SgrBI	CCGCGG	1 cut(s)	53
SinI	GGWCC	3 cut(s)	84, 2302, 2332
SmiMI	CAYNNNNRTG	1 cut(s)	1157
SmlI	CTYRAG	2 cut(s)	1055, 1438
SmoI	CTYRAG	2 cut(s)	1055, 1438
SspDI	GGCGCC	1 cut(s)	268
SspMI	CTAG	2 cut(s)	81, 2794
StyD4I	CCNGG	8 cut(s)	129, 202, 247, 756, 789, 1003, 1121, 2733
StyI	CCWWGG	2 cut(s)	80, 939
TaaI	ACNGT	7 cut(s)	229, 767, 798, 1625, 1702, 2315, 2453
TaiI	ACGT	2 cut(s)	295, 504
TaqI	TCGA	7 cut(s)	231, 303, 309, 514, 786, 935, 1867
TauI	GCSGC	8 cut(s)	55, 198, 404, 421, 535, 818, 821, 2432
Tru1I	TTAA	6 cut(s)	1439, 1521, 1974, 2267, 2424, 2445
Tru9I	TTAA	6 cut(s)	1439, 1521, 1974, 2267, 2424, 2445
TscAI	CASTG	5 cut(s)	1113, 1467, 1630, 1942, 2530
TseFI	GTSAC	4 cut(s)	241, 1009, 1269, 1592
TseI	GCWGC	6 cut(s)	421, 898, 1060, 2153, 2565, 2750
Tsp45I	GTSAC	4 cut(s)	241, 1009, 1269, 1592
TspGWI	ACGGA	3 cut(s)	141, 603, 2199
TspRI	CASTG	5 cut(s)	1113, 1467, 1630, 1942, 2530
Vha464I	CTTAAG	1 cut(s)	1438
VpaK11BI	GGWCC	3 cut(s)	84, 2302, 2332
XapI	RAATTY	9 cut(s)	653, 782, 1196, 1553, 1913, 1959, 2101, 2263, 2621
XceI	RCATGY	1 cut(s)	2094
XmaJI	CCTAGG	1 cut(s)	80
XmiI	GTMKAC	2 cut(s)	335, 1511
XmnI	GAANNNNTTC	1 cut(s)	1782
XspI	CTAG	2 cut(s)	81, 2794
Zsp2I	ATGCAT	2 cut(s)	2096, 2655

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.