MD16G1121900.v1.1

Developmental protein SEPALLATA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006987
Physical Location & Seq
Reverse (-)
8767076 .. 8769741
2666 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1121900.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGGAAGAGGGAGGGTGGAATTGAAGAGGATAGAGAACAAGATCAACAGACAGGTGACCTTTGCGAAGAGAAGGAACGGACTTTTGAAGAAAGCCTACGAGCTTTCCGTTCTTTGCGATGCCGAGGTTGCTCTCATCATCTTCTCCAATAGAGGAAAGCTGTACGAGTTTTGCAGTAGTTCAAGGTTGAGTGTTTCTGCTTCGGCATTGCTGTTAGATCTGCAACAGGAAATAAAGATAAACCCCATCAAAAGAAGATCTCTCATTTCTCATCACTCGTCACTCACTGTTTTTTTTCACAACTCTCCATCGGTCTCAGCTGGTTCTCATCTCTGCTCTTACACTGAAAATTTAGGGCGAGAATTTTTTTCTATTTTTTTAAAAATTAAATTGAGTTTCCTGGAAATATTAATCCAAAAGCAAGAACTTAATTACCCGGTTTTTCCAGTTCCTCATTTGGGGCTTAATATTTTCTCGTATACTGTATTAATTATAAGTTCATTATTTAAGTTTAATTTCTATGAGATTGGCTTGATTTTGTCCTTTTTTTCAATTTTTGTTTTTCGTTTTTTGGTTTCTCAAATGCATGGTAATGTAAAAAAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

203

Amino Acids

23.25

Weight (kDa)

9.93

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.2e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 494
AccI GTMKAC 1 cut(s) 479
AcsI RAATTY 3 cut(s) 349, 362, 602
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 1 cut(s) 458
AgsI TTSAA 4 cut(s) 25, 88, 183, 552
AjnI CCWGG 1 cut(s) 399
AluBI AGCT 3 cut(s) 103, 160, 320
AluI AGCT 3 cut(s) 103, 160, 320
Alw26I GTCTC 1 cut(s) 319
ApoI RAATTY 3 cut(s) 349, 362, 602
AseI ATTAAT 2 cut(s) 410, 488
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 80, 112
BccI CCATC 2 cut(s) 254, 316
BciT130I CCWGG 1 cut(s) 401
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 319
BglII AGATCT 2 cut(s) 217, 257
Bme1390I CCNGG 2 cut(s) 401, 437
BmrFI CCNGG 2 cut(s) 401, 437
BmsI GCATC 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 1 cut(s) 123
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse1I ACTGG 1 cut(s) 446
Bse3DI GCAATG 1 cut(s) 206
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 458
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 1 cut(s) 446
BsiSI CCGG 1 cut(s) 437
BslI CCNNNNNNNGG 1 cut(s) 458
BsmAI GTCTC 1 cut(s) 319
Bso31I GGTCTC 1 cut(s) 319
Bsp143I GATC 3 cut(s) 42, 217, 257
BspCNI CTCAG 1 cut(s) 329
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 206
BsrI ACTGG 1 cut(s) 446
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 3 cut(s) 42, 217, 257
BssNAI GTATAC 1 cut(s) 480
Bst1107I GTATAC 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 2 cut(s) 289, 484
Bst6I CTCTTC 2 cut(s) 20, 62
BstDEI CTNAG 1 cut(s) 316
BstEII GGTNACC 1 cut(s) 55
BstKTI GATC 3 cut(s) 45, 220, 260
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 3 cut(s) 42, 217, 257
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNI CCWGG 1 cut(s) 401
BstPI GGTNACC 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 399, 435
BstX2I RGATCY 2 cut(s) 217, 257
BstYI RGATCY 2 cut(s) 217, 257
BstZ17I GTATAC 1 cut(s) 480
BtgZI GCGATG 1 cut(s) 132
BtsIMutI CAGTG 2 cut(s) 285, 342
Csp6I GTAC 1 cut(s) 163
CviAII CATG 1 cut(s) 587
CviJI RGCY 6 cut(s) 95, 103, 160, 320, 463, 531
CviKI_1 RGCY 6 cut(s) 95, 103, 160, 320, 463, 531
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 1 cut(s) 316
DpnI GATC 3 cut(s) 44, 219, 259
DpnII GATC 3 cut(s) 42, 217, 257
DraI TTTAAA 1 cut(s) 381
Eam1104I CTCTTC 2 cut(s) 20, 62
EarI CTCTTC 2 cut(s) 20, 62
Eco31I GGTCTC 1 cut(s) 319
Eco91I GGTNACC 1 cut(s) 55
EcoO65I GGTNACC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 399
EcoT22I ATGCAT 1 cut(s) 588
FaeI CATG 1 cut(s) 590
FaiI YATR 4 cut(s) 480, 494, 522, 588
FatI CATG 1 cut(s) 586
FblI GTMKAC 1 cut(s) 479
HapII CCGG 1 cut(s) 437
Hin1II CATG 1 cut(s) 590
HpaII CCGG 1 cut(s) 437
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 1 cut(s) 480
Hpy8I GTNNAC 1 cut(s) 480
HpyAV CCTTC 1 cut(s) 66
HpyCH4III ACNGT 2 cut(s) 289, 484
HpyCH4V TGCA 3 cut(s) 174, 223, 586
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 1 cut(s) 316
Hsp92II CATG 1 cut(s) 590
Kzo9I GATC 3 cut(s) 42, 217, 257
LpnPI CCDG 7 cut(s) 38, 212, 306, 386, 413, 450, 459
LweI GCATC 1 cut(s) 109
MaeIII GTNAC 2 cut(s) 55, 279
MalI GATC 3 cut(s) 44, 219, 259
MboI GATC 3 cut(s) 42, 217, 257
MboII GAAGA 6 cut(s) 18, 37, 79, 100, 133, 267
MflI RGATCY 2 cut(s) 217, 257
MnlI CCTC 5 cut(s) 6, 21, 118, 146, 462
Mph1103I ATGCAT 1 cut(s) 588
MseI TTAA 8 cut(s) 380, 387, 410, 429, 465, 488, 507, 513
MslI CAYNNNNRTG 1 cut(s) 591
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 401, 437
MvaI CCWGG 1 cut(s) 401
MwoI GCNNNNNNNGC 1 cut(s) 128
NciI CCSGG 1 cut(s) 437
NdeII GATC 3 cut(s) 42, 217, 257
NlaIII CATG 1 cut(s) 590
NmeAIII GCCGAG 1 cut(s) 148
NmuCI GTSAC 2 cut(s) 55, 279
NsiI ATGCAT 1 cut(s) 588
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfoI TCCNGGA 1 cut(s) 399
PshBI ATTAAT 2 cut(s) 410, 488
PsiI TTATAA 1 cut(s) 494
Psp6I CCWGG 1 cut(s) 399
PspEI GGTNACC 1 cut(s) 55
PspGI CCWGG 1 cut(s) 399
PsuI RGATCY 2 cut(s) 217, 257
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 591
SaqAI TTAA 8 cut(s) 380, 387, 410, 429, 465, 488, 507, 513
Sau3AI GATC 3 cut(s) 42, 217, 257
ScrFI CCNGG 2 cut(s) 401, 437
SetI ASST 7 cut(s) 57, 62, 105, 129, 162, 188, 322
SfaNI GCATC 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 591
SspI AATATT 2 cut(s) 408, 469
StyD4I CCNGG 2 cut(s) 399, 435
TaaI ACNGT 2 cut(s) 289, 484
TaqII GACCGA 1 cut(s) 301
Tru1I TTAA 8 cut(s) 380, 387, 410, 429, 465, 488, 507, 513
Tru9I TTAA 8 cut(s) 380, 387, 410, 429, 465, 488, 507, 513
TscAI CASTG 2 cut(s) 292, 349
TseFI GTSAC 2 cut(s) 55, 279
Tsp45I GTSAC 2 cut(s) 55, 279
TspDTI ATGAA 1 cut(s) 489
TspGWI ACGGA 2 cut(s) 93, 97
TspRI CASTG 2 cut(s) 292, 349
VspI ATTAAT 2 cut(s) 410, 488
XapI RAATTY 3 cut(s) 349, 362, 602
XmiI GTMKAC 1 cut(s) 479
Zsp2I ATGCAT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.