Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010023

Physical Location & Seq

Genomic Coordinates Forward (+)

11579620 .. 11582011

Gene Structure 2392 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1148300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1050 bp

ATGGACATGGCGAAAGGAAGCAGAGGTTGCAAAAGCTCATCATCACAACTCTCGGCAGAAACCCTCAATGAAACTGGCTCTCATGATCATGAGGAAGCTGAGGATCAGATGATGGACCTGCGAAGAGGTCCTTGGACAGTTGAGGAAGACCTAGCTCTCATGAATTACATTGCTAACCACGGCGAAGGCCGCTGGAACTCCCTCGCTGGCTGCGCAGGTCTGAAAAGAACCGGAAAGAGCTGCAGATTACGGTGGCTCAACTATCTCCGCCCCGATGTCCGTCGTGGCAACATCACCCTTGAAGAACAGCTTCTGATTCTTGAGCTTCATTCTCGCTGGGGTAACAGATGGTCAAAAATTGCACAACACTTGCCAGGAAGGACTGACAATGAGATAAAAAACTATTGGAGGACCCGTGTACAAAAACACGCCAAGCAACTCAAATGTGATGTCAACAGCAAGCAGTTCAAGGACACCATGAGGTACATCTGGATGCCTAGATTGGTTGAAAGAATTCAAGCCGCCGCGGCTGCCTCCACCACCACCAACGCCACCGCAACAGATTCCTCTGTCGCCGCCCCGGCTACCTCTCACCACTTCAGCAACAACTTCCAGTCTTCAGCACAAATGTTATTACAGCCAAGTCATCACCATCACTTCGGAGTTTCCTCACATTCGCAACTCACACCGAGTTACAATACCCCGGAGAATTCTAGTACGGCGGCCTCATCGGACTCGTTTGGGACTCAGGTGTCACCTGTTTCCGACCTCACTGATTATTACACCCCTACTACTGCTGCTGCTATCTCGGTTAACAATAACCCTACCCCGGATAATAGTTACTTCGTCCAAGACAATCATCAAGTTGGTAATTACGATAACTACTCGGGGTCCTTAGGTAATCAAGGGTTGGGATTAGAACATTTCCAAACCATGGACGAGCAAAACAACAATTATCAGTGGGGCACTGTGGAAGGCGGGGACATATCGGACAATTGGTGGAATGTTGAGGACATGTGGTTCTTACAACAGCATCTCAGCAGCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006970 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0033993 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1901363 GO:1901700 GO:1902074 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

350

Amino Acids

39.3

Weight (kDa)

5.95

Isoelectric Point (pI)

44.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	42 - 89	1.1e-16	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	45 - 103	8.7e-12	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	95 - 138	1.2e-16	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	99 - 141	5.1e-07	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48000 AT3G06490 AT5G49620 AT5G49620
fragaria_vesca	FvH4_4g20750 FvH4_4g20750
malus_domestica	MD13G1148200.v1.1 MD16G1148300.v1.1
prunus_persica	Prupe.1G111700_v2.0.a1
pyrus_communis	pycom13g12760 pycom16g12660
rosa_chinensis	RchiOBHm_Chr4g0426181
rosa_laevigata	RLG00000007312
rosa_multiflora	Rmu_sc0000730.1_g000023
rosa_roxburghii	Rroxscaffold_5G00368200
rosa_rugosa	Rorug04G0209500
rosa_samantha	Rh4AG266200 Rh4BG272300 Rh4CG285300 Rh4DG268400
rosa_wichuraiana	Rw4G023020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	751
Acc16I	TGCGCA	1 cut(s)	214
Acc36I	ACCTGC	2 cut(s)	126, 206
AccB7I	CCANNNNNTGG	1 cut(s)	934
AccII	CGCG	1 cut(s)	527
AciI	CCGC	9 cut(s)	190, 268, 522, 525, 527, 555, 576, 722, 978
AclWI	GGATC	1 cut(s)	111
AcsI	RAATTY	2 cut(s)	513, 709
AcuI	CTGAAG	2 cut(s)	583, 603
AfaI	GTAC	3 cut(s)	420, 485, 718
AfiI	CCNNNNNNNGG	2 cut(s)	829, 934
AflIII	ACRYGT	1 cut(s)	1014
AgsI	TTSAA	4 cut(s)	302, 469, 509, 518
AjnI	CCWGG	1 cut(s)	373
AjuI	GAANNNNNNNTTGG	2 cut(s)	115, 147
AloI	GAACNNNNNNTCC	2 cut(s)	1004, 1036
AluBI	AGCT	6 cut(s)	36, 98, 155, 240, 310, 325
AluI	AGCT	6 cut(s)	36, 98, 155, 240, 310, 325
AlwI	GGATC	1 cut(s)	111
AlwNI	CAGNNNCTG	1 cut(s)	313
Ama87I	CYCGRG	1 cut(s)	886
AoxI	GGCC	2 cut(s)	187, 723
ApeKI	GCWGC	6 cut(s)	210, 240, 530, 797, 800, 1041
ApoI	RAATTY	2 cut(s)	513, 709
ArsI	GACNNNNNNTTYG	2 cut(s)	435, 467
Asp700I	GAANNNNTTC	2 cut(s)	309, 513
AspLEI	GCGC	1 cut(s)	215
AspS9I	GGNCC	4 cut(s)	115, 128, 411, 891
AsuC2I	CCSGG	3 cut(s)	581, 704, 830
AsuHPI	GGTGA	4 cut(s)	286, 584, 641, 747
AvaI	CYCGRG	1 cut(s)	886
AvaII	GGWCC	4 cut(s)	115, 128, 411, 891
AxyI	CCTNAGG	1 cut(s)	895
BaeGI	GKGCMC	1 cut(s)	968
BbsI	GAAGAC	2 cut(s)	153, 609
BbvCI	CCTCAGC	1 cut(s)	99
BbvI	GCAGC	5 cut(s)	197, 227, 517, 784, 787
BccI	CCATC	3 cut(s)	106, 342, 660
BceAI	ACGGC	2 cut(s)	196, 735
BciT130I	CCWGG	1 cut(s)	375
BclI	TGATCA	1 cut(s)	85
BcnI	CCSGG	3 cut(s)	581, 704, 830
BfaI	CTAG	3 cut(s)	152, 498, 714
BfmI	CTRYAG	1 cut(s)	241
BfuAI	ACCTGC	2 cut(s)	126, 206
BglI	GCCNNNNNGGC	2 cut(s)	527, 581
Bme1390I	CCNGG	4 cut(s)	375, 581, 704, 830
Bme18I	GGWCC	4 cut(s)	115, 128, 411, 891
BmeT110I	CYCGRG	1 cut(s)	886
BmgT120I	GGNCC	4 cut(s)	115, 128, 411, 891
BmiI	GGNNCC	2 cut(s)	413, 892
BmrFI	CCNGG	4 cut(s)	375, 581, 704, 830
BmsI	GCATC	2 cut(s)	483, 1042
BpiI	GAAGAC	2 cut(s)	153, 609
Bpu10I	CCTNAGC	1 cut(s)	99
BpuEI	CTTGAG	1 cut(s)	341
BpuMI	CCSGG	3 cut(s)	581, 704, 830
BsaJI	CCNNGG	7 cut(s)	131, 178, 525, 579, 702, 828, 933
BsaWI	WCCGGW	1 cut(s)	230
BsaXI	ACNNNNNCTCC	2 cut(s)	400, 430
Bsc4I	CCNNNNNNNGG	2 cut(s)	829, 934
Bse1I	ACTGG	2 cut(s)	79, 613
Bse21I	CCTNAGG	1 cut(s)	895
Bse3DI	GCAATG	1 cut(s)	168
BseBI	CCWGG	1 cut(s)	375
BseDI	CCNNGG	7 cut(s)	131, 178, 525, 579, 702, 828, 933
BseGI	GGATG	1 cut(s)	498
BseLI	CCNNNNNNNGG	2 cut(s)	829, 934
BseMI	GCAATG	1 cut(s)	168
BseMII	CTCAG	2 cut(s)	90, 761
BseNI	ACTGG	2 cut(s)	79, 613
BseSI	GKGCMC	1 cut(s)	968
BseXI	GCAGC	5 cut(s)	197, 227, 517, 784, 787
BseYI	CCCAGC	1 cut(s)	336
Bsh1236I	CGCG	1 cut(s)	527
BshFI	GGCC	2 cut(s)	189, 725
BsiHKCI	CYCGRG	1 cut(s)	886
BsiSI	CCGG	4 cut(s)	231, 581, 704, 830
BslFI	GGGAC	2 cut(s)	757, 995
BslI	CCNNNNNNNGG	2 cut(s)	829, 934
BsmFI	GGGAC	2 cut(s)	757, 995
BsnI	GGCC	2 cut(s)	189, 725
BsoBI	CYCGRG	1 cut(s)	886
Bsp1286I	GDGCHC	1 cut(s)	968
Bsp1407I	TGTACA	1 cut(s)	418
Bsp143I	GATC	2 cut(s)	85, 103
Bsp19I	CCATGG	1 cut(s)	933
BspACI	CCGC	9 cut(s)	190, 268, 522, 525, 527, 555, 576, 722, 978
BspANI	GGCC	2 cut(s)	189, 725
BspCNI	CTCAG	3 cut(s)	91, 760, 1050
BspFNI	CGCG	1 cut(s)	527
BspHI	TCATGA	3 cut(s)	82, 88, 159
BspLI	GGNNCC	2 cut(s)	413, 892
BspMAI	CTGCAG	1 cut(s)	245
BspMI	ACCTGC	2 cut(s)	126, 206
BspPI	GGATC	1 cut(s)	111
BsrDI	GCAATG	1 cut(s)	168
BsrGI	TGTACA	1 cut(s)	418
BsrI	ACTGG	2 cut(s)	79, 613
BssECI	CCNNGG	7 cut(s)	131, 178, 525, 579, 702, 828, 933
BssMI	GATC	2 cut(s)	85, 103
BssT1I	CCWWGG	2 cut(s)	131, 933
Bst2UI	CCWGG	1 cut(s)	375
Bst4CI	ACNGT	3 cut(s)	139, 252, 970
Bst6I	CTCTTC	1 cut(s)	118
BstAPI	GCANNNNNTGC	1 cut(s)	27
BstAUI	TGTACA	1 cut(s)	418
BstC8I	GCNNGC	2 cut(s)	208, 461
BstDEI	CTNAG	4 cut(s)	99, 747, 895, 1037
BstDSI	CCRYGG	3 cut(s)	178, 525, 933
BstF5I	GGATG	1 cut(s)	498
BstFNI	CGCG	1 cut(s)	527
BstHHI	GCGC	1 cut(s)	215
BstKTI	GATC	2 cut(s)	88, 106
BstMBI	GATC	2 cut(s)	85, 103
BstMWI	GCNNNNNNNGC	6 cut(s)	27, 189, 212, 527, 530, 581
BstNI	CCWGG	1 cut(s)	375
BstNSI	RCATGY	1 cut(s)	1018
BstSCI	CCNGG	4 cut(s)	373, 579, 702, 828
BstSFI	CTRYAG	1 cut(s)	241
BstSLI	GKGCMC	1 cut(s)	968
BstUI	CGCG	1 cut(s)	527
BstV1I	GCAGC	5 cut(s)	197, 227, 517, 784, 787
BstV2I	GAAGAC	2 cut(s)	153, 609
Bsu36I	CCTNAGG	1 cut(s)	895
BsuRI	GGCC	2 cut(s)	189, 725
BtgI	CCRYGG	3 cut(s)	178, 525, 933
BtsCI	GGATG	1 cut(s)	498
BtsIMutI	CAGTG	3 cut(s)	771, 965, 966
BveI	ACCTGC	2 cut(s)	126, 206
Cac8I	GCNNGC	2 cut(s)	208, 461
CaiI	CAGNNNCTG	1 cut(s)	313
CciI	TCATGA	3 cut(s)	82, 88, 159
CfoI	GCGC	1 cut(s)	215
Cfr13I	GGNCC	4 cut(s)	115, 128, 411, 891
Cfr42I	CCGCGG	1 cut(s)	528
Csp6I	GTAC	3 cut(s)	419, 484, 717
CviAII	CATG	7 cut(s)	7, 83, 89, 160, 478, 934, 1015
CviQI	GTAC	3 cut(s)	419, 484, 717
DdeI	CTNAG	4 cut(s)	99, 747, 895, 1037
DpnI	GATC	2 cut(s)	87, 105
DpnII	GATC	2 cut(s)	85, 103
DrdI	GACNNNNNNGTC	1 cut(s)	751
DseDI	GACNNNNNNGTC	1 cut(s)	751
Eam1104I	CTCTTC	1 cut(s)	118
EarI	CTCTTC	1 cut(s)	118
EciI	GGCGGA	1 cut(s)	257
Eco130I	CCWWGG	2 cut(s)	131, 933
Eco47I	GGWCC	4 cut(s)	115, 128, 411, 891
Eco57I	CTGAAG	2 cut(s)	583, 603
Eco81I	CCTNAGG	1 cut(s)	895
Eco88I	CYCGRG	1 cut(s)	886
EcoO109I	RGGNCCY	3 cut(s)	128, 411, 891
EcoRI	GAATTC	2 cut(s)	513, 709
EcoRII	CCWGG	1 cut(s)	373
EcoT14I	CCWWGG	2 cut(s)	131, 933
ErhI	CCWWGG	2 cut(s)	131, 933
FaeI	CATG	7 cut(s)	10, 86, 92, 163, 481, 937, 1018
FaiI	YATR	8 cut(s)	8, 84, 90, 161, 479, 935, 986, 1016
FalI	AAGNNNNNCTT	4 cut(s)	115, 147, 294, 326
FaqI	GGGAC	2 cut(s)	757, 995
FatI	CATG	7 cut(s)	6, 82, 88, 159, 477, 933, 1014
FauI	CCCGC	1 cut(s)	971
FbaI	TGATCA	1 cut(s)	85
FokI	GGATG	1 cut(s)	505
FspBI	CTAG	3 cut(s)	152, 498, 714
FspI	TGCGCA	1 cut(s)	214
GlaI	GCGC	1 cut(s)	214
GsaI	CCCAGC	1 cut(s)	340
HaeIII	GGCC	2 cut(s)	189, 725
HapII	CCGG	4 cut(s)	231, 581, 704, 830
HhaI	GCGC	1 cut(s)	215
Hin1II	CATG	7 cut(s)	10, 86, 92, 163, 481, 937, 1018
Hin6I	GCGC	1 cut(s)	213
HinP1I	GCGC	1 cut(s)	213
HincII	GTYRAC	2 cut(s)	454, 814
HindII	GTYRAC	2 cut(s)	454, 814
HinfI	GANTC	4 cut(s)	316, 563, 734, 745
HpaI	GTTAAC	1 cut(s)	814
HpaII	CCGG	4 cut(s)	231, 581, 704, 830
HphI	GGTGA	4 cut(s)	286, 584, 641, 747
Hpy166II	GTNNAC	3 cut(s)	419, 454, 814
Hpy188I	TCNGA	7 cut(s)	108, 222, 315, 662, 733, 766, 991
Hpy188III	TCNNGA	5 cut(s)	83, 89, 160, 320, 490
Hpy8I	GTNNAC	3 cut(s)	419, 454, 814
Hpy99I	CGWCG	1 cut(s)	285
HpyAV	CCTTC	3 cut(s)	179, 372, 968
HpyCH4III	ACNGT	3 cut(s)	139, 252, 970
HpyCH4V	TGCA	3 cut(s)	30, 243, 362
HpyF10VI	GCNNNNNNNGC	6 cut(s)	27, 189, 212, 527, 530, 581
HpyF3I	CTNAG	4 cut(s)	99, 747, 895, 1037
Hsp92II	CATG	7 cut(s)	10, 86, 92, 163, 481, 937, 1018
HspAI	GCGC	1 cut(s)	213
Ksp22I	TGATCA	1 cut(s)	85
KspAI	GTTAAC	1 cut(s)	814
KspI	CCGCGG	1 cut(s)	528
Kzo9I	GATC	2 cut(s)	85, 103
Lsp1109I	GCAGC	5 cut(s)	197, 227, 517, 784, 787
LweI	GCATC	2 cut(s)	483, 1042
MaeI	CTAG	3 cut(s)	152, 498, 714
MaeIII	GTNAC	4 cut(s)	341, 692, 753, 839
MalI	GATC	2 cut(s)	87, 105
MboI	GATC	2 cut(s)	85, 103
MboII	GAAGA	4 cut(s)	135, 158, 314, 609
MfeI	CAATTG	1 cut(s)	994
MhlI	GDGCHC	1 cut(s)	968
MluCI	AATT	7 cut(s)	163, 357, 513, 709, 871, 952, 994
MlyI	GAGTC	2 cut(s)	728, 739
MmeI	TCCRAC	1 cut(s)	789
MroXI	GAANNNNTTC	2 cut(s)	309, 513
MseI	TTAA	1 cut(s)	813
MslI	CAYNNNNRTG	2 cut(s)	87, 491
MspA1I	CMGCKG	2 cut(s)	192, 527
MspI	CCGG	4 cut(s)	231, 581, 704, 830
MspR9I	CCNGG	4 cut(s)	375, 581, 704, 830
MunI	CAATTG	1 cut(s)	994
MvaI	CCWGG	1 cut(s)	375
MvnI	CGCG	1 cut(s)	527
MwoI	GCNNNNNNNGC	6 cut(s)	27, 189, 212, 527, 530, 581
NciI	CCSGG	3 cut(s)	581, 704, 830
NcoI	CCATGG	1 cut(s)	933
NdeII	GATC	2 cut(s)	85, 103
NlaIII	CATG	7 cut(s)	10, 86, 92, 163, 481, 937, 1018
NlaIV	GGNNCC	2 cut(s)	413, 892
NmeAIII	GCCGAG	1 cut(s)	32
NmuCI	GTSAC	1 cut(s)	753
NsbI	TGCGCA	1 cut(s)	214
NspI	RCATGY	1 cut(s)	1018
PagI	TCATGA	3 cut(s)	82, 88, 159
PciI	ACATGT	1 cut(s)	1014
PdmI	GAANNNNTTC	2 cut(s)	309, 513
PfeI	GAWTC	2 cut(s)	316, 563
PflMI	CCANNNNNTGG	1 cut(s)	934
PleI	GAGTC	2 cut(s)	728, 739
PpsI	GAGTC	2 cut(s)	728, 739
PpuMI	RGGWCCY	3 cut(s)	128, 411, 891
PscI	ACATGT	1 cut(s)	1014
Psp5II	RGGWCCY	3 cut(s)	128, 411, 891
Psp6I	CCWGG	1 cut(s)	373
PspFI	CCCAGC	1 cut(s)	336
PspGI	CCWGG	1 cut(s)	373
PspN4I	GGNNCC	2 cut(s)	413, 892
PspPI	GGNCC	4 cut(s)	115, 128, 411, 891
PspPPI	RGGWCCY	3 cut(s)	128, 411, 891
PstI	CTGCAG	1 cut(s)	245
PstNI	CAGNNNCTG	1 cut(s)	313
RsaI	GTAC	3 cut(s)	420, 485, 718
RsaNI	GTAC	3 cut(s)	419, 484, 717
RseI	CAYNNNNRTG	2 cut(s)	87, 491
SacII	CCGCGG	1 cut(s)	528
SaqAI	TTAA	1 cut(s)	813
Sau3AI	GATC	2 cut(s)	85, 103
Sau96I	GGNCC	4 cut(s)	115, 128, 411, 891
SchI	GAGTC	2 cut(s)	728, 739
ScrFI	CCNGG	4 cut(s)	375, 581, 704, 830
SduI	GDGCHC	1 cut(s)	968
SfaNI	GCATC	2 cut(s)	483, 1042
SfcI	CTRYAG	1 cut(s)	241
Sfr303I	CCGCGG	1 cut(s)	528
SgrBI	CCGCGG	1 cut(s)	528
SinI	GGWCC	4 cut(s)	115, 128, 411, 891
SmiMI	CAYNNNNRTG	2 cut(s)	87, 491
SmlI	CTYRAG	1 cut(s)	320
SmoI	CTYRAG	1 cut(s)	320
Sse9I	AATT	7 cut(s)	163, 357, 513, 709, 871, 952, 994
SsiI	CCGC	9 cut(s)	190, 268, 522, 525, 527, 555, 576, 722, 978
SspMI	CTAG	3 cut(s)	152, 498, 714
StyD4I	CCNGG	4 cut(s)	373, 579, 702, 828
StyI	CCWWGG	2 cut(s)	131, 933
TaaI	ACNGT	3 cut(s)	139, 252, 970
TasI	AATT	7 cut(s)	163, 357, 513, 709, 871, 952, 994
TatI	WGTACW	1 cut(s)	418
TauI	GCSGC	6 cut(s)	192, 524, 527, 530, 578, 725
TfiI	GAWTC	2 cut(s)	316, 563
Tru1I	TTAA	1 cut(s)	813
Tru9I	TTAA	1 cut(s)	813
TscAI	CASTG	3 cut(s)	778, 965, 973
TseFI	GTSAC	1 cut(s)	753
TseI	GCWGC	6 cut(s)	210, 240, 530, 797, 800, 1041
Tsp45I	GTSAC	1 cut(s)	753
TspDTI	ATGAA	3 cut(s)	84, 176, 317
TspGWI	ACGGA	1 cut(s)	269
TspRI	CASTG	3 cut(s)	778, 965, 973
Van91I	CCANNNNNTGG	1 cut(s)	934
VpaK11BI	GGWCC	4 cut(s)	115, 128, 411, 891
XapI	RAATTY	2 cut(s)	513, 709
XceI	RCATGY	1 cut(s)	1018
XmnI	GAANNNNTTC	2 cut(s)	309, 513
XspI	CTAG	3 cut(s)	152, 498, 714

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.