Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0010023

Physical Location & Seq

Genomic Coordinates Forward (+)

8963714 .. 8965659

Gene Structure 1946 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom13g12760.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1248 bp

ATGCTTAACTTTTTCTCCATTTTCAAACCTATCCTTTCATCTATAAAAGCCATCCCCTCTCTGTTTCCCGTACATCCATCCAACTCTCTCTCTCTCTCTCTCAAAATCTCCAAGAACAAAGCAAGGAAAGCATTAAGCAAACCCTTTTTTTTTGTGTGTCCATTTGGAGAATTGATATTCTGGAGATGGACATGGCGAAAGGAAGCAGAGATTGCAACAGCCCATCATCACAATTCTCGGCAGAAATCCTCAATGAAACTGGCTCTTATGATCGATGAGGAAGCTGAGGATCAGATGATGGACCTGCGAAGAGGGCCTTGGACTGTTGAGGAAGACCTAGCTCTCATGAATTACATTGCTAACCACGGTGAAGGCCGCTGGAACTCCCTCGCTCGCTGCGCAGGTCTGAAAAGAACCGGAAAGAGCTGCAGATTGCGGTGGCTCAACTATCTCCGCCCTGATGTCCGTCGTGGTAACATCAGTCTTGAAGAACAGCTTCTGATTCTGGAACTTCATTCTCGCTGGGGAAACAGATGGTCGAAAATTGCACAGCAATTGCCAGGAAGGACTGACAATGAGATAAAAAACTATTGGAGGACCCGTGTCCAAAAACACGCCAAGCAACTCAAATGTGATGTCAACAGCAAGCAGTTCAAGGACACCATGAGATACCTCTGGATGCCTAGATTGGTCGAAAGAATCCAAGCCGCCGCGGCCACCTCCACTGCCATCAACGCCACCGCCACTAATTCCTCCATCGCGGCCCCAGCCACCACTCACCACTTCAACAACAACATTAACAACAACTTCCAATCTTCAGCACAAATGTTATTACAGCCAAGTGACCATCAGTTCGGGGTTTCATCACACTCCCAACTCACACCGAGTTACAATACTCCGGAGAATTCTAGTACGGCGGCCTCATCGGACTCGTTTGGGACTCAGGTATCACCTGTTTCCGACCTCACTGATTATTACACCCCTACTACTGCTGCTGCGGCTATCTCGGTTAACAATAACCATACCCCGGATAATAGTTACTTCGTCCAGGCCAATCATCAAGTTGGTACTTACGACAACTACTCGGGGTACTTCGGTAATCAAGGGTTCGGATTAGAACATTTCCAAAACATGGACGAGCAAAACAACAATGGTCAGTGGGGCACTGTGGACGGTGGGGACATATCGGACAATTTGTGGAATGTTGAAGACATGTGGTTCTTACAACAGCAGCTCAGCAACATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006970 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0033993 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1901363 GO:1901700 GO:1902074 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

416

Amino Acids

47.26

Weight (kDa)

8.75

Isoelectric Point (pI)

44.36

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48000 AT3G06490 AT5G49620 AT5G49620
fragaria_vesca	FvH4_4g20750 FvH4_4g20750
malus_domestica	MD13G1148200.v1.1 MD16G1148300.v1.1
prunus_persica	Prupe.1G111700_v2.0.a1
pyrus_communis	pycom13g12760 pycom16g12660
rosa_chinensis	RchiOBHm_Chr4g0426181
rosa_laevigata	RLG00000007312
rosa_multiflora	Rmu_sc0000730.1_g000023
rosa_roxburghii	Rroxscaffold_5G00368200
rosa_rugosa	Rorug04G0209500
rosa_samantha	Rh4AG266200 Rh4BG272300 Rh4CG285300 Rh4DG268400
rosa_wichuraiana	Rw4G023020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	400
Acc36I	ACCTGC	2 cut(s)	312, 392
AccB7I	CCANNNNNTGG	1 cut(s)	1132
AccII	CGCG	2 cut(s)	713, 761
AccIII	TCCGGA	1 cut(s)	898
AclWI	GGATC	1 cut(s)	297
AcoI	YGGCCR	1 cut(s)	714
AcsI	RAATTY	1 cut(s)	904
AcuI	CTGAAG	1 cut(s)	801
AfaI	GTAC	4 cut(s)	72, 913, 1069, 1091
AfiI	CCNNNNNNNGG	2 cut(s)	1027, 1132
AflIII	ACRYGT	1 cut(s)	1212
AgsI	TTSAA	5 cut(s)	25, 488, 655, 787, 1208
AjnI	CCWGG	2 cut(s)	559, 1047
AjuI	GAANNNNNNNTTGG	2 cut(s)	301, 333
AluBI	AGCT	5 cut(s)	284, 341, 426, 496, 1234
AluI	AGCT	5 cut(s)	284, 341, 426, 496, 1234
AlwI	GGATC	1 cut(s)	297
AlwNI	CAGNNNCTG	1 cut(s)	499
Ama87I	CYCGRG	1 cut(s)	1084
Aor13HI	TCCGGA	1 cut(s)	898
AoxI	GGCC	6 cut(s)	314, 373, 714, 762, 918, 1050
ApeKI	GCWGC	5 cut(s)	396, 426, 992, 995, 1231
ApoI	RAATTY	1 cut(s)	904
ArsI	GACNNNNNNTTYG	4 cut(s)	621, 653, 836, 868
Asp700I	GAANNNNTTC	1 cut(s)	495
AspLEI	GCGC	1 cut(s)	401
AspS9I	GGNCC	4 cut(s)	301, 314, 597, 763
AsuC2I	CCSGG	1 cut(s)	1028
AsuHPI	GGTGA	3 cut(s)	380, 770, 942
AvaI	CYCGRG	1 cut(s)	1084
AvaII	GGWCC	2 cut(s)	301, 597
BaeGI	GKGCMC	1 cut(s)	1166
BaeI	ACNNNNGTAYC	4 cut(s)	930, 963, 1081, 1114
BbsI	GAAGAC	2 cut(s)	339, 1215
BbvCI	CCTCAGC	1 cut(s)	285
BbvI	GCAGC	5 cut(s)	383, 413, 979, 982, 1243
BccI	CCATC	9 cut(s)	59, 85, 180, 231, 292, 528, 737, 764, 855
BceAI	ACGGC	1 cut(s)	930
BciT130I	CCWGG	2 cut(s)	561, 1049
BcnI	CCSGG	1 cut(s)	1028
BfaI	CTAG	3 cut(s)	338, 684, 909
BfmI	CTRYAG	1 cut(s)	427
BfuAI	ACCTGC	2 cut(s)	312, 392
BglI	GCCNNNNNGGC	1 cut(s)	713
BlpI	GCTNAGC	1 cut(s)	1235
Bme1390I	CCNGG	3 cut(s)	561, 1028, 1049
Bme18I	GGWCC	2 cut(s)	301, 597
BmeT110I	CYCGRG	1 cut(s)	1084
BmgT120I	GGNCC	4 cut(s)	301, 314, 597, 763
BmiI	GGNNCC	2 cut(s)	599, 765
BmrFI	CCNGG	3 cut(s)	561, 1028, 1049
BmsI	GCATC	1 cut(s)	669
BoxI	GACNNNNGTC	1 cut(s)	602
BpiI	GAAGAC	2 cut(s)	339, 1215
BpmI	CTGGAG	1 cut(s)	202
Bpu10I	CCTNAGC	1 cut(s)	285
Bpu1102I	GCTNAGC	1 cut(s)	1235
BpuMI	CCSGG	1 cut(s)	1028
Bsa29I	ATCGAT	1 cut(s)	273
BsaJI	CCNNGG	4 cut(s)	317, 364, 711, 1026
BsaWI	WCCGGW	2 cut(s)	416, 898
BsaXI	ACNNNNNCTCC	3 cut(s)	29, 586, 616
Bsc4I	CCNNNNNNNGG	2 cut(s)	1027, 1132
Bse1I	ACTGG	1 cut(s)	264
Bse3DI	GCAATG	1 cut(s)	354
BseAI	TCCGGA	1 cut(s)	898
BseBI	CCWGG	2 cut(s)	561, 1049
BseCI	ATCGAT	1 cut(s)	273
BseDI	CCNNGG	4 cut(s)	317, 364, 711, 1026
BseGI	GGATG	4 cut(s)	51, 73, 77, 684
BseLI	CCNNNNNNNGG	2 cut(s)	1027, 1132
BseMI	GCAATG	1 cut(s)	354
BseMII	CTCAG	2 cut(s)	276, 956
BseNI	ACTGG	1 cut(s)	264
BseSI	GKGCMC	1 cut(s)	1166
BseXI	GCAGC	5 cut(s)	383, 413, 979, 982, 1243
BseYI	CCCAGC	2 cut(s)	522, 766
Bsh1236I	CGCG	2 cut(s)	713, 761
BshFI	GGCC	6 cut(s)	316, 375, 716, 764, 920, 1052
BshVI	ATCGAT	1 cut(s)	273
BsiHKCI	CYCGRG	1 cut(s)	1084
BsiSI	CCGG	3 cut(s)	417, 899, 1028
BslFI	GGGAC	2 cut(s)	952, 1193
BslI	CCNNNNNNNGG	2 cut(s)	1027, 1132
BsmFI	GGGAC	2 cut(s)	952, 1193
BsnI	GGCC	6 cut(s)	316, 375, 716, 764, 920, 1052
BsoBI	CYCGRG	1 cut(s)	1084
Bsp1286I	GDGCHC	1 cut(s)	1166
Bsp13I	TCCGGA	1 cut(s)	898
Bsp143I	GATC	2 cut(s)	270, 289
Bsp1720I	GCTNAGC	1 cut(s)	1235
BspANI	GGCC	6 cut(s)	316, 375, 716, 764, 920, 1052
BspCNI	CTCAG	3 cut(s)	277, 955, 1248
BspDI	ATCGAT	1 cut(s)	273
BspEI	TCCGGA	1 cut(s)	898
BspFNI	CGCG	2 cut(s)	713, 761
BspHI	TCATGA	1 cut(s)	345
BspLI	GGNNCC	2 cut(s)	599, 765
BspMAI	CTGCAG	1 cut(s)	431
BspMI	ACCTGC	2 cut(s)	312, 392
BspPI	GGATC	1 cut(s)	297
BsrDI	GCAATG	1 cut(s)	354
BsrI	ACTGG	1 cut(s)	264
BssECI	CCNNGG	4 cut(s)	317, 364, 711, 1026
BssMI	GATC	2 cut(s)	270, 289
BssT1I	CCWWGG	1 cut(s)	317
Bst2UI	CCWGG	2 cut(s)	561, 1049
Bst4CI	ACNGT	4 cut(s)	325, 368, 1168, 1175
Bst6I	CTCTTC	1 cut(s)	304
BstAPI	GCANNNNNTGC	1 cut(s)	212
BstC8I	GCNNGC	2 cut(s)	394, 647
BstDEI	CTNAG	3 cut(s)	285, 942, 1235
BstDSI	CCRYGG	2 cut(s)	364, 711
BstF5I	GGATG	4 cut(s)	51, 73, 77, 684
BstFNI	CGCG	2 cut(s)	713, 761
BstHHI	GCGC	1 cut(s)	401
BstKTI	GATC	2 cut(s)	273, 292
BstMBI	GATC	2 cut(s)	270, 289
BstMWI	GCNNNNNNNGC	8 cut(s)	128, 212, 313, 398, 713, 734, 767, 998
BstNI	CCWGG	2 cut(s)	561, 1049
BstNSI	RCATGY	1 cut(s)	1216
BstPAI	GACNNNNGTC	1 cut(s)	602
BstSCI	CCNGG	3 cut(s)	559, 1026, 1047
BstSFI	CTRYAG	1 cut(s)	427
BstSLI	GKGCMC	1 cut(s)	1166
BstUI	CGCG	2 cut(s)	713, 761
BstV1I	GCAGC	5 cut(s)	383, 413, 979, 982, 1243
BstV2I	GAAGAC	2 cut(s)	339, 1215
Bsu15I	ATCGAT	1 cut(s)	273
BsuRI	GGCC	6 cut(s)	316, 375, 716, 764, 920, 1052
BsuTUI	ATCGAT	1 cut(s)	273
BtgI	CCRYGG	2 cut(s)	364, 711
BtgZI	GCGATG	1 cut(s)	742
BtsCI	GGATG	4 cut(s)	51, 73, 77, 684
BtsI	GCAGTG	1 cut(s)	723
BtsIMutI	CAGTG	4 cut(s)	723, 966, 1163, 1164
BveI	ACCTGC	2 cut(s)	312, 392
Cac8I	GCNNGC	2 cut(s)	394, 647
CaiI	CAGNNNCTG	1 cut(s)	499
CciI	TCATGA	1 cut(s)	345
CfoI	GCGC	1 cut(s)	401
Cfr13I	GGNCC	4 cut(s)	301, 314, 597, 763
Cfr42I	CCGCGG	1 cut(s)	714
ClaI	ATCGAT	1 cut(s)	273
Csp6I	GTAC	4 cut(s)	71, 912, 1068, 1090
CviAII	CATG	5 cut(s)	192, 346, 664, 1132, 1213
CviQI	GTAC	4 cut(s)	71, 912, 1068, 1090
DdeI	CTNAG	3 cut(s)	285, 942, 1235
DpnI	GATC	2 cut(s)	272, 291
DpnII	GATC	2 cut(s)	270, 289
EaeI	YGGCCR	1 cut(s)	714
Eam1104I	CTCTTC	1 cut(s)	304
EarI	CTCTTC	1 cut(s)	304
EciI	GGCGGA	1 cut(s)	443
Eco130I	CCWWGG	1 cut(s)	317
Eco47I	GGWCC	2 cut(s)	301, 597
Eco57I	CTGAAG	1 cut(s)	801
Eco88I	CYCGRG	1 cut(s)	1084
EcoO109I	RGGNCCY	2 cut(s)	314, 597
EcoRI	GAATTC	1 cut(s)	904
EcoRII	CCWGG	2 cut(s)	559, 1047
EcoT14I	CCWWGG	1 cut(s)	317
ErhI	CCWWGG	1 cut(s)	317
FaeI	CATG	5 cut(s)	195, 349, 667, 1135, 1216
FaiI	YATR	9 cut(s)	44, 193, 269, 347, 665, 1023, 1133, 1184, 1214
FalI	AAGNNNNNCTT	4 cut(s)	301, 333, 480, 512
FaqI	GGGAC	2 cut(s)	952, 1193
FatI	CATG	5 cut(s)	191, 345, 663, 1131, 1212
FokI	GGATG	4 cut(s)	38, 60, 64, 691
FspBI	CTAG	3 cut(s)	338, 684, 909
FspI	TGCGCA	1 cut(s)	400
GlaI	GCGC	1 cut(s)	400
GsaI	CCCAGC	2 cut(s)	526, 770
GsuI	CTGGAG	1 cut(s)	202
HaeIII	GGCC	6 cut(s)	316, 375, 716, 764, 920, 1052
HapII	CCGG	3 cut(s)	417, 899, 1028
HhaI	GCGC	1 cut(s)	401
Hin1II	CATG	5 cut(s)	195, 349, 667, 1135, 1216
Hin6I	GCGC	1 cut(s)	399
HinP1I	GCGC	1 cut(s)	399
HincII	GTYRAC	2 cut(s)	640, 1012
HindII	GTYRAC	2 cut(s)	640, 1012
HinfI	GANTC	4 cut(s)	502, 699, 929, 940
HpaI	GTTAAC	1 cut(s)	1012
HpaII	CCGG	3 cut(s)	417, 899, 1028
HphI	GGTGA	3 cut(s)	380, 770, 942
Hpy166II	GTNNAC	3 cut(s)	640, 1012, 1171
Hpy188I	TCNGA	7 cut(s)	294, 408, 501, 928, 961, 1112, 1189
Hpy188III	TCNNGA	6 cut(s)	181, 346, 485, 506, 676, 899
Hpy8I	GTNNAC	3 cut(s)	640, 1012, 1171
Hpy99I	CGWCG	1 cut(s)	471
HpyAV	CCTTC	2 cut(s)	365, 558
HpyCH4III	ACNGT	4 cut(s)	325, 368, 1168, 1175
HpyCH4V	TGCA	3 cut(s)	215, 429, 548
HpyF10VI	GCNNNNNNNGC	8 cut(s)	128, 212, 313, 398, 713, 734, 767, 998
HpyF3I	CTNAG	3 cut(s)	285, 942, 1235
Hsp92II	CATG	5 cut(s)	195, 349, 667, 1135, 1216
HspAI	GCGC	1 cut(s)	399
Kpn2I	TCCGGA	1 cut(s)	898
KspAI	GTTAAC	1 cut(s)	1012
KspI	CCGCGG	1 cut(s)	714
Kzo9I	GATC	2 cut(s)	270, 289
Lsp1109I	GCAGC	5 cut(s)	383, 413, 979, 982, 1243
LweI	GCATC	1 cut(s)	669
MaeI	CTAG	3 cut(s)	338, 684, 909
MaeIII	GTNAC	4 cut(s)	473, 842, 887, 1037
MalI	GATC	2 cut(s)	272, 291
MboI	GATC	2 cut(s)	270, 289
MboII	GAAGA	5 cut(s)	321, 344, 500, 807, 1220
MfeI	CAATTG	1 cut(s)	554
MhlI	GDGCHC	1 cut(s)	1166
MluCI	AATT	8 cut(s)	170, 232, 349, 543, 554, 748, 904, 1192
MlyI	GAGTC	2 cut(s)	923, 934
MmeI	TCCRAC	2 cut(s)	105, 984
MroI	TCCGGA	1 cut(s)	898
MroXI	GAANNNNTTC	1 cut(s)	495
MseI	TTAA	4 cut(s)	6, 134, 798, 1011
MspA1I	CMGCKG	2 cut(s)	378, 713
MspI	CCGG	3 cut(s)	417, 899, 1028
MspR9I	CCNGG	3 cut(s)	561, 1028, 1049
MunI	CAATTG	1 cut(s)	554
MvaI	CCWGG	2 cut(s)	561, 1049
MvnI	CGCG	2 cut(s)	713, 761
MwoI	GCNNNNNNNGC	8 cut(s)	128, 212, 313, 398, 713, 734, 767, 998
NciI	CCSGG	1 cut(s)	1028
NdeII	GATC	2 cut(s)	270, 289
NlaIII	CATG	5 cut(s)	195, 349, 667, 1135, 1216
NlaIV	GGNNCC	2 cut(s)	599, 765
NmeAIII	GCCGAG	1 cut(s)	217
NmuCI	GTSAC	1 cut(s)	842
NsbI	TGCGCA	1 cut(s)	400
NspI	RCATGY	1 cut(s)	1216
PagI	TCATGA	1 cut(s)	345
PciI	ACATGT	1 cut(s)	1212
PdmI	GAANNNNTTC	1 cut(s)	495
PfeI	GAWTC	2 cut(s)	502, 699
PflMI	CCANNNNNTGG	1 cut(s)	1132
PleI	GAGTC	2 cut(s)	923, 934
PpsI	GAGTC	2 cut(s)	923, 934
PpuMI	RGGWCCY	1 cut(s)	597
PscI	ACATGT	1 cut(s)	1212
PshAI	GACNNNNGTC	1 cut(s)	602
Psp5II	RGGWCCY	1 cut(s)	597
Psp6I	CCWGG	2 cut(s)	559, 1047
PspFI	CCCAGC	2 cut(s)	522, 766
PspGI	CCWGG	2 cut(s)	559, 1047
PspN4I	GGNNCC	2 cut(s)	599, 765
PspPI	GGNCC	4 cut(s)	301, 314, 597, 763
PspPPI	RGGWCCY	1 cut(s)	597
PstI	CTGCAG	1 cut(s)	431
PstNI	CAGNNNCTG	1 cut(s)	499
RsaI	GTAC	4 cut(s)	72, 913, 1069, 1091
RsaNI	GTAC	4 cut(s)	71, 912, 1068, 1090
SacII	CCGCGG	1 cut(s)	714
SaqAI	TTAA	4 cut(s)	6, 134, 798, 1011
Sau3AI	GATC	2 cut(s)	270, 289
Sau96I	GGNCC	4 cut(s)	301, 314, 597, 763
SchI	GAGTC	2 cut(s)	923, 934
ScrFI	CCNGG	3 cut(s)	561, 1028, 1049
SduI	GDGCHC	1 cut(s)	1166
SfaNI	GCATC	1 cut(s)	669
SfcI	CTRYAG	1 cut(s)	427
Sfr303I	CCGCGG	1 cut(s)	714
SgrBI	CCGCGG	1 cut(s)	714
SinI	GGWCC	2 cut(s)	301, 597
Sse9I	AATT	8 cut(s)	170, 232, 349, 543, 554, 748, 904, 1192
SspMI	CTAG	3 cut(s)	338, 684, 909
StyD4I	CCNGG	3 cut(s)	559, 1026, 1047
StyI	CCWWGG	1 cut(s)	317
TaaI	ACNGT	4 cut(s)	325, 368, 1168, 1175
TaqI	TCGA	3 cut(s)	273, 539, 693
TasI	AATT	8 cut(s)	170, 232, 349, 543, 554, 748, 904, 1192
TauI	GCSGC	7 cut(s)	378, 710, 713, 716, 764, 920, 1001
TfiI	GAWTC	2 cut(s)	502, 699
Tru1I	TTAA	4 cut(s)	6, 134, 798, 1011
Tru9I	TTAA	4 cut(s)	6, 134, 798, 1011
TscAI	CASTG	4 cut(s)	730, 973, 1163, 1171
TseFI	GTSAC	1 cut(s)	842
TseI	GCWGC	5 cut(s)	396, 426, 992, 995, 1231
Tsp45I	GTSAC	1 cut(s)	842
TspDTI	ATGAA	5 cut(s)	27, 269, 362, 503, 852
TspGWI	ACGGA	1 cut(s)	455
TspRI	CASTG	4 cut(s)	730, 973, 1163, 1171
Van91I	CCANNNNNTGG	1 cut(s)	1132
VpaK11BI	GGWCC	2 cut(s)	301, 597
XapI	RAATTY	1 cut(s)	904
XceI	RCATGY	1 cut(s)	1216
XmnI	GAANNNNTTC	1 cut(s)	495
XspI	CTAG	3 cut(s)	338, 684, 909

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.