Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010320

Physical Location & Seq

Genomic Coordinates Forward (+)

13219384 .. 13224505

Gene Structure 5122 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1162000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1965 bp

ATGGCGGCGAAGAAGCAGGAGAAGAGCTCGCCAAAGAAGAGGAAGCAAATCCCAGGCAATAAGCCGGAGACCAACAGCTCGACTTCTAAGAAGCCCAAGCTCCTCGACTCCAAGCCCTCAAATCCTCGAAGCACCGACTTCAAGAAACCCTCCAAACCTTTCAAGCCCCGAGAACCGGGACTCGATCATGAGAAACAAGTTCCGCTTTCGAAGCGAGAGGGCCGCCTCCGTGCCAAGGAGCTTGCAGAGGCTAGGAAGAAAAAGCGGAAGCGTCATTACAATTTAGAGCAAGAGCTTGCACATCTGTGGGAAAAGATGCGGCAACGAAATATTTCCAAAGAAGATCGATCCAAGTTGGTGAGTGAAGCGGTAGAGAAAATGAAGGGGAAAATTCCTGAAATCGCAAGCTCCCATGTGTCTTCTCGTGTTCTGCAGACTTGCATTAAGTACTGTTCACAAGCTGAAAAGGATGCAGTATTTGAGGAGCTTCAGCCACATCTTCTTACTCTTGCATGCAACACGTATGCTGTTCATCTGGTGACCAAAATGTTAGACAATGCCTCCAAAATGCAGCTAGCGGCATTTATCTCATCTCTCCGAGGACATGTTGCTTCCCTTCTTCGTCACATGGTTGGTTCTGTAGTCGTTGAGCATGCATACCAATTGGGAAATGCAACTCAAAAGCATGAACTTTTGGTGGAACTATATTCTACGGAGCTTCAGTTGTTTAAGGACTTGGTCTCGAAGAATGAGGGCAGGTTACTAGATATAATTTCAAAGCTAGATCTGCAGAAATCTTCAGTGTTGCGGCACATGACCTCAGTGATTCAACCAATTTTAGAGAAAGGAATAATTGATCACTCTATAGTACACAGGGTGTTGATAGAGTACTTCACAATAGCTGAACAGTTCTCTGCCACAGATGTAATTAAACAGTTGTCGGGCCCACTTCTTGTTCGGGTGATCCACACAAAGGATGGATCTAAGGTTGGGATGCTCTGTGTCAAGCATGGCAGTTCAAAGGAAAGAAAGAAGATAATCAAAGGAATGAAAGGCCATGTTCATAAAATAGCTCTTGATCAAGGTGGAAGTATGGTGCTTGTTTGCCTCGTTTCAACTGTTGATGATACGAAGCTTACTACAAAGGTTGTCATTCATGAACTTCAAGAAAATCTAAAGGATCTTGTTCTTGATAAGAATGGAAGGCGCCCGTTACTGCAGTTACTTCATCCAAATTGTTCACGCTATTTGACTCCCGATGACCTGGCTTCCCTCAGTTTGTCTATCCCTTCTCTTTCCAACAAGGCTGGACATGACAATTCTGAGACAAAATCTTCGAAGGTTAACAACTCTGGTGAGGAGGGCAGTAGTGATTTGGAATTAGATGAAGCAGATATGAATGCTGAGGATGGTCTCCACTCAGTTGAGGGGGGAAAAAAGGATCCTGCCATTCGAAGGCAGGAGTTGTTAGTCCAGAGTGGGCTGGCTGAGAAGCTAGTTGATGTATGCATTACAAGTGCCGGGGAATTGCTTAGATCAAATTTTGGCAAAGAAGTCATATATGAGGTTGCAACTGGGGGTGCCGGTGGAATTCTCCACCCAAGTTTGGATGACAAGTTGAGTGAGTTATATGAAACTATAGCATCTCTTGTAGCAGAACCGAAATCCCAAGAATCCAAAGAGGACTCAAAAGAGGAACACATCCTTGAAAATTTCCATTCCAGTCGGACCATAAGAAAACTAATCTTGGACTGCCCCACATTTGCTTCCACTTTGTGGAACAAAGCACTCAAAGGAGAGTGTGAGTTGTGGGCCCACGGTCACAGCGGAAAGGTAATTGCTGCATTCTTAGAATCCTCAGACTCTAAGGTCAATCAGCTGGCAAAGAAAGAGTTGAAGAAGTTGATAGACGGTGGCATTCTCAAAATGCCCGATCCAAAAGTAGCCACCGGAAAGAAGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006417 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0010608 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032268 GO:0032501 GO:0032502 GO:0034248 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0080090 GO:0097159 GO:1901363 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CPL zf-XS

Protein Analysis

655

Amino Acids

72.68

Weight (kDa)

9.19

Isoelectric Point (pI)

41.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF_NOP9	PF22493	135 - 402	6.1e-12	NOP9-like PUF repeat domain
CPL	PF08144	477 - 582	3.9e-10	CPL (NUC119) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010320)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16810
fragaria_vesca	FvH4_4g18900 FvH4_4g18900
malus_domestica	MD13G1162200.v1.1 MD16G1162000.v1.1
prunus_persica	Prupe.1G129500_v2.0.a1
pyrus_communis	pycom13g13840 pycom16g13730 pycom16g13740
rosa_chinensis	RchiOBHm_Chr4g0423801
rosa_laevigata	RLG00000007532
rosa_multiflora	Rmu_co8056970.1_g000001 Rmu_sc0003369.1_g000020
rosa_roxburghii	Rroxscaffold_5G00365910
rosa_rugosa	Rorug04G0190800
rosa_samantha	Rh4AG250200 Rh4BG255400 Rh4CG064700 Rh4CG263400 Rh4DG249700
rosa_wichuraiana	Rw4G021420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1868
Acc36I	ACCTGC	1 cut(s)	747
AccB1I	GGYRCC	2 cut(s)	1206, 1580
AccB7I	CCANNNNNTGG	1 cut(s)	1776
AciI	CCGC	9 cut(s)	5, 203, 223, 265, 319, 368, 578, 808, 1827
AclWI	GGATC	7 cut(s)	342, 958, 988, 1188, 1436, 1449, 1928
AcsI	RAATTY	4 cut(s)	390, 1540, 1590, 1711
AcuI	CTGAAG	3 cut(s)	473, 704, 783
AcyI	GRCGYC	1 cut(s)	1207
AdeI	CACNNNGTG	2 cut(s)	877, 1776
AfaI	GTAC	3 cut(s)	449, 870, 890
AfiI	CCNNNNNNNGG	6 cut(s)	175, 235, 973, 1455, 1606, 1776
AflIII	ACRYGT	2 cut(s)	519, 604
AgsI	TTSAA	9 cut(s)	142, 163, 777, 830, 1020, 1116, 1166, 1709, 1897
AjnI	CCWGG	2 cut(s)	52, 1263
AleI	CACNNNNGTG	1 cut(s)	304
Alw21I	GWGCWC	1 cut(s)	29
Alw26I	GTCTC	4 cut(s)	62, 745, 1319, 1418
AlwI	GGATC	7 cut(s)	342, 958, 988, 1188, 1436, 1449, 1928
Ama87I	CYCGRG	1 cut(s)	168
AoxI	GGCC	4 cut(s)	220, 943, 1054, 1812
ApaI	GGGCCC	2 cut(s)	947, 1816
ApeKI	GCWGC	2 cut(s)	571, 1841
ApoI	RAATTY	4 cut(s)	390, 1540, 1590, 1711
ArsI	GACNNNNNNTTYG	2 cut(s)	1318, 1350
Asp700I	GAANNNNTTC	1 cut(s)	331
AspLEI	GCGC	1 cut(s)	1209
AspS9I	GGNCC	6 cut(s)	220, 943, 944, 1728, 1812, 1813
AsuC2I	CCSGG	2 cut(s)	177, 1522
AsuHPI	GGTGA	4 cut(s)	370, 550, 973, 1367
AsuII	TTCGAA	3 cut(s)	209, 1337, 1453
AsuNHI	GCTAGC	1 cut(s)	574
AvaI	CYCGRG	1 cut(s)	168
AvaII	GGWCC	1 cut(s)	1728
BaeGI	GKGCMC	2 cut(s)	947, 1816
BamHI	GGATCC	1 cut(s)	1441
BanI	GGYRCC	2 cut(s)	1206, 1580
BanII	GRGCYC	3 cut(s)	29, 947, 1816
BarI	GAAGNNNNNNTAC	2 cut(s)	260, 292
BauI	CACGAG	1 cut(s)	423
BbsI	GAAGAC	1 cut(s)	411
Bbv12I	GWGCWC	1 cut(s)	29
BbvCI	CCTCAGC	1 cut(s)	1404
BbvI	GCAGC	2 cut(s)	583, 1828
BccI	CCATC	2 cut(s)	971, 1403
BciT130I	CCWGG	2 cut(s)	54, 1265
BclI	TGATCA	2 cut(s)	856, 1078
BcnI	CCSGG	2 cut(s)	177, 1522
BcoDI	GTCTC	4 cut(s)	62, 745, 1319, 1418
BfaI	CTAG	5 cut(s)	252, 575, 764, 782, 1496
BfmI	CTRYAG	6 cut(s)	431, 639, 788, 864, 1217, 1638
BfoI	RGCGCY	1 cut(s)	1210
BfuAI	ACCTGC	1 cut(s)	747
BglII	AGATCT	1 cut(s)	784
BisI	GCNGC	7 cut(s)	6, 223, 320, 572, 579, 809, 1842
BlsI	GCNGC	7 cut(s)	7, 224, 321, 573, 580, 810, 1843
BmcAI	AGTACT	2 cut(s)	449, 890
Bme1390I	CCNGG	4 cut(s)	54, 177, 1265, 1522
Bme18I	GGWCC	1 cut(s)	1728
BmeT110I	CYCGRG	1 cut(s)	168
BmgT120I	GGNCC	6 cut(s)	220, 943, 944, 1728, 1812, 1813
BmiI	GGNNCC	5 cut(s)	945, 1208, 1443, 1582, 1814
BmrFI	CCNGG	4 cut(s)	54, 177, 1265, 1522
BmrI	ACTGGG	1 cut(s)	1584
BmsI	GCATC	4 cut(s)	306, 460, 984, 1652
BmtI	GCTAGC	1 cut(s)	578
BmuI	ACTGGG	1 cut(s)	1584
BpiI	GAAGAC	1 cut(s)	411
BplI	GAGNNNNNCTC	2 cut(s)	11, 43
Bpu10I	CCTNAGC	1 cut(s)	1404
Bpu14I	TTCGAA	3 cut(s)	209, 1337, 1453
BpuMI	CCSGG	2 cut(s)	177, 1522
Bsa29I	ATCGAT	1 cut(s)	346
BsaAI	YACGTR	1 cut(s)	522
BsaHI	GRCGYC	1 cut(s)	1207
BsaI	GGTCTC	3 cut(s)	62, 745, 1418
BsaJI	CCNNGG	5 cut(s)	52, 234, 598, 1521, 1816
BsaWI	WCCGGW	1 cut(s)	1949
BsaXI	ACNNNNNCTCC	4 cut(s)	545, 575, 707, 737
Bsc4I	CCNNNNNNNGG	6 cut(s)	175, 235, 973, 1455, 1606, 1776
Bse118I	RCCGGY	1 cut(s)	1583
Bse1I	ACTGG	2 cut(s)	1579, 1722
BseBI	CCWGG	2 cut(s)	54, 1265
BseCI	ATCGAT	1 cut(s)	346
BseDI	CCNNGG	5 cut(s)	52, 234, 598, 1521, 1816
BseGI	GGATG	7 cut(s)	475, 982, 999, 1228, 1414, 1615, 1701
BseLI	CCNNNNNNNGG	6 cut(s)	175, 235, 973, 1455, 1606, 1776
BseMII	CTCAG	7 cut(s)	834, 1288, 1314, 1395, 1434, 1479, 1872
BseNI	ACTGG	2 cut(s)	1579, 1722
BseRI	GAGGAG	3 cut(s)	92, 497, 1373
BseSI	GKGCMC	2 cut(s)	947, 1816
BseXI	GCAGC	2 cut(s)	583, 1828
BshFI	GGCC	4 cut(s)	222, 945, 1056, 1814
BshNI	GGYRCC	2 cut(s)	1206, 1580
BshVI	ATCGAT	1 cut(s)	346
BsiHKAI	GWGCWC	1 cut(s)	29
BsiHKCI	CYCGRG	1 cut(s)	168
BsiSI	CCGG	5 cut(s)	65, 176, 1521, 1584, 1950
BslFI	GGGAC	1 cut(s)	192
BslI	CCNNNNNNNGG	6 cut(s)	175, 235, 973, 1455, 1606, 1776
BsmAI	GTCTC	4 cut(s)	62, 745, 1319, 1418
BsmFI	GGGAC	1 cut(s)	192
BsmI	GAATGC	3 cut(s)	1405, 1844, 1917
BsnI	GGCC	4 cut(s)	222, 945, 1056, 1814
Bso31I	GGTCTC	3 cut(s)	62, 745, 1418
BsoBI	CYCGRG	1 cut(s)	168
Bsp119I	TTCGAA	3 cut(s)	209, 1337, 1453
Bsp120I	GGGCCC	2 cut(s)	943, 1812
Bsp1286I	GDGCHC	3 cut(s)	29, 947, 1816
BspACI	CCGC	9 cut(s)	5, 203, 223, 265, 319, 368, 578, 808, 1827
BspANI	GGCC	4 cut(s)	222, 945, 1056, 1814
BspCNI	CTCAG	7 cut(s)	833, 1287, 1315, 1396, 1433, 1480, 1871
BspDI	ATCGAT	1 cut(s)	346
BspHI	TCATGA	2 cut(s)	187, 1156
BspLI	GGNNCC	5 cut(s)	945, 1208, 1443, 1582, 1814
BspMAI	CTGCAG	3 cut(s)	435, 792, 1221
BspMI	ACCTGC	1 cut(s)	747
BspOI	GCTAGC	1 cut(s)	578
BspPI	GGATC	7 cut(s)	342, 958, 988, 1188, 1436, 1449, 1928
BspQI	GCTCTTC	1 cut(s)	17
BspT104I	TTCGAA	3 cut(s)	209, 1337, 1453
BspT107I	GGYRCC	2 cut(s)	1206, 1580
BspTNI	GGTCTC	3 cut(s)	62, 745, 1418
BsrFI	RCCGGY	1 cut(s)	1583
BsrI	ACTGG	2 cut(s)	1579, 1722
BssAI	RCCGGY	1 cut(s)	1583
BssECI	CCNNGG	5 cut(s)	52, 234, 598, 1521, 1816
BssNI	GRCGYC	1 cut(s)	1207
BssSI	CACGAG	1 cut(s)	423
BssT1I	CCWWGG	1 cut(s)	234
Bst2BI	CACGAG	1 cut(s)	423
Bst2UI	CCWGG	2 cut(s)	54, 1265
Bst4CI	ACNGT	6 cut(s)	452, 909, 936, 1120, 1820, 1913
Bst6I	CTCTTC	2 cut(s)	17, 32
BstACI	GRCGYC	1 cut(s)	1207
BstBAI	YACGTR	1 cut(s)	522
BstBI	TTCGAA	3 cut(s)	209, 1337, 1453
BstC8I	GCNNGC	9 cut(s)	29, 243, 297, 406, 514, 576, 654, 1485, 1881
BstDSI	CCRYGG	1 cut(s)	1816
BstEII	GGTNACC	1 cut(s)	538
BstF5I	GGATG	7 cut(s)	475, 982, 999, 1228, 1414, 1615, 1701
BstH2I	RGCGCY	1 cut(s)	1210
BstHHI	GCGC	1 cut(s)	1209
BstMAI	GTCTC	4 cut(s)	62, 745, 1319, 1418
BstMWI	GCNNNNNNNGC	2 cut(s)	211, 787
BstNI	CCWGG	2 cut(s)	54, 1265
BstNSI	RCATGY	3 cut(s)	516, 608, 656
BstPI	GGTNACC	1 cut(s)	538
BstSCI	CCNGG	4 cut(s)	52, 175, 1263, 1520
BstSFI	CTRYAG	6 cut(s)	431, 639, 788, 864, 1217, 1638
BstSLI	GKGCMC	2 cut(s)	947, 1816
BstV1I	GCAGC	2 cut(s)	583, 1828
BstV2I	GAAGAC	1 cut(s)	411
BstX2I	RGATCY	4 cut(s)	784, 980, 1180, 1441
BstYI	RGATCY	4 cut(s)	784, 980, 1180, 1441
Bsu15I	ATCGAT	1 cut(s)	346
BsuRI	GGCC	4 cut(s)	222, 945, 1056, 1814
BsuTUI	ATCGAT	1 cut(s)	346
BtgI	CCRYGG	1 cut(s)	1816
BtsCI	GGATG	7 cut(s)	475, 982, 999, 1228, 1414, 1615, 1701
BtsIMutI	CAGTG	2 cut(s)	807, 828
BveI	ACCTGC	1 cut(s)	747
Cac8I	GCNNGC	9 cut(s)	29, 243, 297, 406, 514, 576, 654, 1485, 1881
CciI	TCATGA	2 cut(s)	187, 1156
CfoI	GCGC	1 cut(s)	1209
Cfr10I	RCCGGY	1 cut(s)	1583
Cfr13I	GGNCC	6 cut(s)	220, 943, 944, 1728, 1812, 1813
ClaI	ATCGAT	1 cut(s)	346
CseI	GACGC	1 cut(s)	260
Csp6I	GTAC	3 cut(s)	448, 869, 889
CviQI	GTAC	3 cut(s)	448, 869, 889
DinI	GGCGCC	1 cut(s)	1208
DraIII	CACNNNGTG	2 cut(s)	877, 1776
DrdI	GACNNNNNNGTC	1 cut(s)	1868
DseDI	GACNNNNNNGTC	1 cut(s)	1868
Eam1104I	CTCTTC	2 cut(s)	17, 32
EarI	CTCTTC	2 cut(s)	17, 32
Ecl136II	GAGCTC	1 cut(s)	27
Eco130I	CCWWGG	1 cut(s)	234
Eco24I	GRGCYC	3 cut(s)	29, 947, 1816
Eco31I	GGTCTC	3 cut(s)	62, 745, 1418
Eco47I	GGWCC	1 cut(s)	1728
Eco53kI	GAGCTC	1 cut(s)	27
Eco57I	CTGAAG	3 cut(s)	473, 704, 783
Eco88I	CYCGRG	1 cut(s)	168
Eco91I	GGTNACC	1 cut(s)	538
EcoICRI	GAGCTC	1 cut(s)	27
EcoO65I	GGTNACC	1 cut(s)	538
EcoRI	GAATTC	1 cut(s)	1590
EcoRII	CCWGG	2 cut(s)	52, 1263
EcoT14I	CCWWGG	1 cut(s)	234
EcoT22I	ATGCAT	2 cut(s)	658, 1511
EcoT38I	GRGCYC	3 cut(s)	29, 947, 1816
EgeI	GGCGCC	1 cut(s)	1208
EheI	GGCGCC	1 cut(s)	1208
ErhI	CCWWGG	1 cut(s)	234
FalI	AAGNNNNNCTT	2 cut(s)	189, 221
FaqI	GGGAC	1 cut(s)	192
FbaI	TGATCA	2 cut(s)	856, 1078
Fnu4HI	GCNGC	7 cut(s)	6, 223, 320, 572, 579, 809, 1842
FokI	GGATG	7 cut(s)	482, 989, 1006, 1215, 1421, 1622, 1688
FriOI	GRGCYC	3 cut(s)	29, 947, 1816
Fsp4HI	GCNGC	7 cut(s)	6, 223, 320, 572, 579, 809, 1842
FspBI	CTAG	5 cut(s)	252, 575, 764, 782, 1496
GlaI	GCGC	1 cut(s)	1208
GluI	GCNGC	7 cut(s)	6, 223, 320, 572, 579, 809, 1842
HaeII	RGCGCY	1 cut(s)	1210
HaeIII	GGCC	4 cut(s)	222, 945, 1056, 1814
HapII	CCGG	5 cut(s)	65, 176, 1521, 1584, 1950
HgaI	GACGC	1 cut(s)	260
HhaI	GCGC	1 cut(s)	1209
Hin1I	GRCGYC	1 cut(s)	1207
Hin6I	GCGC	1 cut(s)	1207
HinP1I	GCGC	1 cut(s)	1207
HincII	GTYRAC	1 cut(s)	1345
HindII	GTYRAC	1 cut(s)	1345
HindIII	AAGCTT	1 cut(s)	1133
HinfI	GANTC	8 cut(s)	107, 180, 826, 1252, 1673, 1685, 1853, 1862
HpaI	GTTAAC	1 cut(s)	1345
HpaII	CCGG	5 cut(s)	65, 176, 1521, 1584, 1950
HphI	GGTGA	4 cut(s)	370, 550, 973, 1367
Hpy166II	GTNNAC	4 cut(s)	455, 871, 1241, 1345
Hpy188I	TCNGA	4 cut(s)	599, 1324, 1728, 1861
Hpy8I	GTNNAC	4 cut(s)	455, 871, 1241, 1345
HpyAV	CCTTC	7 cut(s)	376, 626, 1197, 1299, 1333, 1449, 1951
HpyCH4III	ACNGT	6 cut(s)	452, 909, 936, 1120, 1820, 1913
HpyCH4IV	ACGT	1 cut(s)	521
HpyF10VI	GCNNNNNNNGC	2 cut(s)	211, 787
HpySE526I	ACGT	1 cut(s)	521
Hsp92I	GRCGYC	1 cut(s)	1207
HspAI	GCGC	1 cut(s)	1207
KasI	GGCGCC	1 cut(s)	1206
Ksp22I	TGATCA	2 cut(s)	856, 1078
KspAI	GTTAAC	1 cut(s)	1345
LguI	GCTCTTC	1 cut(s)	17
LmnI	GCTCC	5 cut(s)	105, 238, 413, 484, 715
Lsp1109I	GCAGC	2 cut(s)	583, 1828
LweI	GCATC	4 cut(s)	306, 460, 984, 1652
MaeI	CTAG	5 cut(s)	252, 575, 764, 782, 1496
MaeII	ACGT	1 cut(s)	521
MaeIII	GTNAC	6 cut(s)	538, 623, 759, 1212, 1221, 1820
MfeI	CAATTG	1 cut(s)	662
MflI	RGATCY	4 cut(s)	784, 980, 1180, 1441
MhlI	GDGCHC	3 cut(s)	29, 947, 1816
Mly113I	GGCGCC	1 cut(s)	1207
MlyI	GAGTC	5 cut(s)	101, 174, 1246, 1679, 1856
MmeI	TCCRAC	2 cut(s)	1323, 1706
Mph1103I	ATGCAT	2 cut(s)	658, 1511
MroXI	GAANNNNTTC	1 cut(s)	331
MseI	TTAA	4 cut(s)	444, 729, 930, 1344
MslI	CAYNNNNRTG	1 cut(s)	304
MspA1I	CMGCKG	2 cut(s)	1827, 1879
MspI	CCGG	5 cut(s)	65, 176, 1521, 1584, 1950
MspR9I	CCNGG	4 cut(s)	54, 177, 1265, 1522
MunI	CAATTG	1 cut(s)	662
Mva1269I	GAATGC	3 cut(s)	1405, 1844, 1917
MvaI	CCWGG	2 cut(s)	54, 1265
MwoI	GCNNNNNNNGC	2 cut(s)	211, 787
NarI	GGCGCC	1 cut(s)	1207
NciI	CCSGG	2 cut(s)	177, 1522
NheI	GCTAGC	1 cut(s)	574
NlaIV	GGNNCC	5 cut(s)	945, 1208, 1443, 1582, 1814
NmuCI	GTSAC	3 cut(s)	538, 623, 1820
NsiI	ATGCAT	2 cut(s)	658, 1511
NspI	RCATGY	3 cut(s)	516, 608, 656
NspV	TTCGAA	3 cut(s)	209, 1337, 1453
OliI	CACNNNNGTG	1 cut(s)	304
PaeI	GCATGC	2 cut(s)	516, 656
PagI	TCATGA	2 cut(s)	187, 1156
PciI	ACATGT	1 cut(s)	604
PciSI	GCTCTTC	1 cut(s)	17
PctI	GAATGC	3 cut(s)	1405, 1844, 1917
PdmI	GAANNNNTTC	1 cut(s)	331
PfeI	GAWTC	3 cut(s)	826, 1673, 1853
PflFI	GACNNNGTC	1 cut(s)	737
PflMI	CCANNNNNTGG	1 cut(s)	1776
PkrI	GCNGC	7 cut(s)	7, 224, 321, 573, 580, 810, 1843
PleI	GAGTC	5 cut(s)	101, 174, 1246, 1679, 1856
PluTI	GGCGCC	1 cut(s)	1210
PpsI	GAGTC	5 cut(s)	101, 174, 1246, 1679, 1856
Ppu21I	YACGTR	1 cut(s)	522
PscI	ACATGT	1 cut(s)	604
Psp124BI	GAGCTC	1 cut(s)	29
Psp6I	CCWGG	2 cut(s)	52, 1263
PspEI	GGTNACC	1 cut(s)	538
PspGI	CCWGG	2 cut(s)	52, 1263
PspN4I	GGNNCC	5 cut(s)	945, 1208, 1443, 1582, 1814
PspOMI	GGGCCC	2 cut(s)	943, 1812
PspPI	GGNCC	6 cut(s)	220, 943, 944, 1728, 1812, 1813
PstI	CTGCAG	3 cut(s)	435, 792, 1221
PsuI	RGATCY	4 cut(s)	784, 980, 1180, 1441
PsyI	GACNNNGTC	1 cut(s)	737
PvuII	CAGCTG	1 cut(s)	1879
RsaI	GTAC	3 cut(s)	449, 870, 890
RsaNI	GTAC	3 cut(s)	448, 869, 889
RseI	CAYNNNNRTG	1 cut(s)	304
SacI	GAGCTC	1 cut(s)	29
SapI	GCTCTTC	1 cut(s)	17
SaqAI	TTAA	4 cut(s)	444, 729, 930, 1344
SatI	GCNGC	7 cut(s)	6, 223, 320, 572, 579, 809, 1842
Sau96I	GGNCC	6 cut(s)	220, 943, 944, 1728, 1812, 1813
ScaI	AGTACT	2 cut(s)	449, 890
SchI	GAGTC	5 cut(s)	101, 174, 1246, 1679, 1856
ScrFI	CCNGG	4 cut(s)	54, 177, 1265, 1522
SduI	GDGCHC	3 cut(s)	29, 947, 1816
SfaNI	GCATC	4 cut(s)	306, 460, 984, 1652
SfcI	CTRYAG	6 cut(s)	431, 639, 788, 864, 1217, 1638
SfoI	GGCGCC	1 cut(s)	1208
SfuI	TTCGAA	3 cut(s)	209, 1337, 1453
SinI	GGWCC	1 cut(s)	1728
SmiMI	CAYNNNNRTG	1 cut(s)	304
SphI	GCATGC	2 cut(s)	516, 656
SsiI	CCGC	9 cut(s)	5, 203, 223, 265, 319, 368, 578, 808, 1827
SspDI	GGCGCC	1 cut(s)	1206
SspI	AATATT	1 cut(s)	331
SspMI	CTAG	5 cut(s)	252, 575, 764, 782, 1496
SstI	GAGCTC	1 cut(s)	29
StyD4I	CCNGG	4 cut(s)	52, 175, 1263, 1520
StyI	CCWWGG	1 cut(s)	234
TaaI	ACNGT	6 cut(s)	452, 909, 936, 1120, 1820, 1913
TaiI	ACGT	1 cut(s)	524
TaqI	TCGA	9 cut(s)	80, 105, 127, 183, 209, 346, 743, 1337, 1453
TatI	WGTACW	3 cut(s)	447, 868, 888
TauI	GCSGC	5 cut(s)	8, 225, 322, 581, 811
TfiI	GAWTC	3 cut(s)	826, 1673, 1853
Tru1I	TTAA	4 cut(s)	444, 729, 930, 1344
Tru9I	TTAA	4 cut(s)	444, 729, 930, 1344
TscAI	CASTG	2 cut(s)	807, 828
TseFI	GTSAC	3 cut(s)	538, 623, 1820
TseI	GCWGC	2 cut(s)	571, 1841
Tsp45I	GTSAC	3 cut(s)	538, 623, 1820
TspGWI	ACGGA	2 cut(s)	218, 728
TspRI	CASTG	2 cut(s)	807, 828
Tth111I	GACNNNGTC	1 cut(s)	737
Van91I	CCANNNNNTGG	1 cut(s)	1776
VpaK11BI	GGWCC	1 cut(s)	1728
XapI	RAATTY	4 cut(s)	390, 1540, 1590, 1711
XceI	RCATGY	3 cut(s)	516, 608, 656
XcmI	CCANNNNNNNNNTGG	1 cut(s)	974
XmnI	GAANNNNTTC	1 cut(s)	331
XspI	CTAG	5 cut(s)	252, 575, 764, 782, 1496
ZrmI	AGTACT	2 cut(s)	449, 890
Zsp2I	ATGCAT	2 cut(s)	658, 1511

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.