Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0010320

Physical Location & Seq

Genomic Coordinates Reverse (-)

46094504 .. 46098996

Gene Structure 4493 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G021420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1959 bp

ATGGCGGCCAAGGACCACGACACCAGACTAAAGAAGCGGAAGCAAGTTTCCGGCGGTACTAAGCCGGGGACCCACCATACCTCCACTTCCAAGAAACCCAAGCTCGTCGGCTCAAAGCCCACCAATCCTCGAAACAATGACTCCTCTAAACCCTTCAAACGCCCCAAACCGGCGGGGCCTGACAACGCCGAGAAAACGACGCCGTTGTCGAAGAAAGAGCTTCGCGTCCGCGCTAAGGAGCTTGCAGAAGCTAGGAAGAAGAAGCGAAAGCGTTATTACAATTTAGAGCATGAGCTTGCGCATCTGTGGGAGAAGATGAGGAGTCGAGATATTTCCAAGGAAGATAGATATAAGTTGGTTAGCGAAGCACTTGAAAAAATGAAGGGGAAAATTCCTGAAATTGCTAGCTCCCATGTTTCTTCCCGTGTTCTGCAGACTTGTGTTAAGTACTGCACGAAAGCAGAAAGGGATGCAAAAAAGAAAAAAGCGGACGGGGATGCAAAAAAGAGAAAAGCAGAAAAGGATGCAGTATTTGAGGAGCTTAAGCCGCATTTTCTTACCCTTTCACGCAGTGCATATGGTGTTCATCTGGTGAAAAAGATGTTAGATAATGCCTCCAAACAGCAGCTTGCGGAGTTTATTTCGTCACTTCATGGACATGTTGCATCCCTTCTGCGTCACATGGTTGGATCTGTAGTCGTAGAGCATGCATACCAATTGGGAAATGCAACTCAAAAACAAGAACTTTTGGTGGAACTATATTCTACAGAACTACAGTTGTTTAAGGACTTGGTCTTGAAGAAAGAGGGAAGGTTAGTAGATATAATATCAAAGTTAGATCTGCAGAAATCTTCAGTCCTGAAGCACATGACTTCGGTGATTCAACCAATTTTAGAGAAAGGAATAGTTGACCACTCTATATTACATAGGGTGTTGATAGAGTACTTCACCATAGCTGAAGAGTTCTCTGCCACAGATGTCATTAAACAGTTGTCAGGTCCTCTTCTTGTCCGAATGATTCATACAAGGGATGGATCTAGGGTTGGAATGCTCTGTGTCAAGTATGGCAGTGCAAAGGAAAGAAAGAAGATAATCAAAGGAATGAAAGGCCACATACGTAATATAGCTAGTGATCAATGTGGAAGTATGGTGCTTGTTTCTCTTGTTTCAGTTGTTGATGACACGAAGCTTATTACAAAGGTTGTCATTAATGAGCTTCAAGAACATTTAAAGGATCTTGTTTTTGATAAGGATGGAAGGCGTCCGTTGCTACAGCTACTTCATCCAAATTGTTCACGCTATTTGACTCCCTATGACCTGGCTTCTCTCAGTTTGTCTATCCCTTCTTTATCCAACAAGGTTGAGTCAGACATCCCTTCTGAAGGGATGGAAACTGAAACTAATGATCCTGATGAGAGTACCCAATTAGTTGATGGTGGAAAAAAAGATCCTTCAGTTCGAAGGCAAGAATTGTTAGTCAAGAGTGGGCTTGCTGAGAGGCTAGTTGATGTATGCATTGCAAGTGCAGGGGAGTTGCTTAGATCAAACTTTGGCAAAGAAGTCATGTATGAGGTTGCAACTGGGGGTGCTGATGGAATCCTCCACCCAACTTTGGATGACAAGTTGAATGAACTATATGAAGCTATAGCAACTCTTGTCGCAGAGCCCAAATCCGAAGAACCAAAAGAAGAACACCTCCTTGAAAATTTCCATTCTAGTCGGACCATTAGAAGACTAATCTTGGATTGCCCTGCATTCGCTTCCACTCTGTGGAATAAGGCACTGAAAGGGAAATGTGAATTGTGGGCCCAAGGTCACAGCTGTAGGGTAGTCTCTGCATTCTTAGCATCTTCAGACTCTAAGGTAAACAAGCTGGCAAAGAAGGAGTTGCAGCCACTGATAGATGCTGGTACTCTGAAAGTCCCCGAGACACGAGCAGCCATTGGAACCAAGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006417 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0010608 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032268 GO:0032501 GO:0032502 GO:0034248 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0080090 GO:0097159 GO:1901363 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CPL zf-XS

Protein Analysis

652

Amino Acids

72.86

Weight (kDa)

9.42

Isoelectric Point (pI)

34.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPL	PF08144	481 - 580	1.8e-08	CPL (NUC119) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010320)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16810
fragaria_vesca	FvH4_4g18900 FvH4_4g18900
malus_domestica	MD13G1162200.v1.1 MD16G1162000.v1.1
prunus_persica	Prupe.1G129500_v2.0.a1
pyrus_communis	pycom13g13840 pycom16g13730 pycom16g13740
rosa_chinensis	RchiOBHm_Chr4g0423801
rosa_laevigata	RLG00000007532
rosa_multiflora	Rmu_co8056970.1_g000001 Rmu_sc0003369.1_g000020
rosa_roxburghii	Rroxscaffold_5G00365910
rosa_rugosa	Rorug04G0190800
rosa_samantha	Rh4AG250200 Rh4BG255400 Rh4CG064700 Rh4CG263400 Rh4DG249700
rosa_wichuraiana	Rw4G021420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	205
Acc16I	TGCGCA	1 cut(s)	300
AccB7I	CCANNNNNTGG	1 cut(s)	1770
AccII	CGCG	2 cut(s)	225, 231
AciI	CCGC	8 cut(s)	5, 37, 54, 173, 229, 488, 548, 632
AclWI	GGATC	5 cut(s)	697, 1042, 1242, 1400, 1442
AcoI	YGGCCR	1 cut(s)	6
AcsI	RAATTY	2 cut(s)	390, 1705
AcuI	CTGAAG	6 cut(s)	837, 881, 978, 1401, 1437, 1836
AcyI	GRCGYC	2 cut(s)	200, 1261
AdeI	CACNNNGTG	2 cut(s)	572, 1770
AfaI	GTAC	5 cut(s)	58, 449, 944, 1420, 1912
AfiI	CCNNNNNNNGG	5 cut(s)	169, 235, 1382, 1612, 1770
AflII	CTTAAG	1 cut(s)	542
AflIII	ACRYGT	1 cut(s)	658
AgsI	TTSAA	7 cut(s)	157, 374, 799, 884, 1220, 1627, 1703
AjnI	CCWGG	1 cut(s)	1317
Alw26I	GTCTC	2 cut(s)	1837, 1922
AlwI	GGATC	5 cut(s)	697, 1042, 1242, 1400, 1442
AlwNI	CAGNNNCTG	1 cut(s)	1897
Ama87I	CYCGRG	1 cut(s)	1925
AoxI	GGCC	4 cut(s)	6, 176, 1108, 1806
ApaI	GGGCCC	1 cut(s)	1810
ApeKI	GCWGC	3 cut(s)	625, 1891, 1937
ApoI	RAATTY	2 cut(s)	390, 1705
AseI	ATTAAT	1 cut(s)	1209
AspLEI	GCGC	2 cut(s)	233, 301
AspS9I	GGNCC	7 cut(s)	13, 69, 176, 998, 1722, 1806, 1807
AsuC2I	CCSGG	1 cut(s)	66
AsuHPI	GGTGA	3 cut(s)	604, 889, 940
AsuII	TTCGAA	1 cut(s)	1459
AsuNHI	GCTAGC	1 cut(s)	404
AvaI	CYCGRG	1 cut(s)	1925
AvaII	GGWCC	4 cut(s)	13, 69, 998, 1722
BaeGI	GKGCMC	1 cut(s)	1810
BanII	GRGCYC	2 cut(s)	1668, 1810
BauI	CACGAG	1 cut(s)	1932
BbsI	GAAGAC	1 cut(s)	1738
BbvI	GCAGC	3 cut(s)	637, 1903, 1949
BccI	CCATC	5 cut(s)	1025, 1247, 1381, 1427, 1586
BceAI	ACGGC	1 cut(s)	187
BciT130I	CCWGG	1 cut(s)	1319
BclI	TGATCA	1 cut(s)	1132
BcnI	CCSGG	1 cut(s)	66
BcoDI	GTCTC	2 cut(s)	1837, 1922
BfaI	CTAG	6 cut(s)	252, 405, 1038, 1128, 1502, 1716
BfmI	CTRYAG	8 cut(s)	431, 693, 765, 773, 842, 1271, 1644, 1822
BfrI	CTTAAG	1 cut(s)	542
BglII	AGATCT	1 cut(s)	838
BisI	GCNGC	5 cut(s)	6, 548, 626, 1892, 1938
BlsI	GCNGC	5 cut(s)	7, 549, 627, 1893, 1939
BmcAI	AGTACT	2 cut(s)	449, 944
Bme1390I	CCNGG	2 cut(s)	66, 1319
Bme18I	GGWCC	4 cut(s)	13, 69, 998, 1722
BmeT110I	CYCGRG	1 cut(s)	1925
BmgT120I	GGNCC	7 cut(s)	13, 69, 176, 998, 1722, 1806, 1807
BmiI	GGNNCC	5 cut(s)	70, 71, 177, 1808, 1948
BmrFI	CCNGG	2 cut(s)	66, 1319
BmrI	ACTGGG	1 cut(s)	1590
BmsI	GCATC	7 cut(s)	310, 460, 487, 514, 674, 1856, 1894
BmtI	GCTAGC	1 cut(s)	408
BmuI	ACTGGG	1 cut(s)	1590
BpiI	GAAGAC	1 cut(s)	1738
Bpu10I	CCTNAGC	1 cut(s)	234
Bpu14I	TTCGAA	1 cut(s)	1459
BpuMI	CCSGG	1 cut(s)	66
BsaAI	YACGTR	1 cut(s)	1118
BsaHI	GRCGYC	2 cut(s)	200, 1261
BsaJI	CCNNGG	5 cut(s)	9, 65, 336, 1810, 1950
BsaXI	ACNNNNNCTCC	4 cut(s)	65, 95, 125, 155
Bsc4I	CCNNNNNNNGG	5 cut(s)	169, 235, 1382, 1612, 1770
Bse118I	RCCGGY	1 cut(s)	169
Bse1I	ACTGG	1 cut(s)	1585
Bse3DI	GCAATG	1 cut(s)	1515
BseBI	CCWGG	1 cut(s)	1319
BseDI	CCNNGG	5 cut(s)	9, 65, 336, 1810, 1950
BseLI	CCNNNNNNNGG	5 cut(s)	169, 235, 1382, 1612, 1770
BseMI	GCAATG	1 cut(s)	1515
BseMII	CTCAG	2 cut(s)	1342, 1485
BseNI	ACTGG	1 cut(s)	1585
BseRI	GAGGAG	3 cut(s)	133, 334, 551
BseSI	GKGCMC	1 cut(s)	1810
BseXI	GCAGC	3 cut(s)	637, 1903, 1949
BsgI	GTGCAG	2 cut(s)	436, 1545
Bsh1236I	CGCG	2 cut(s)	225, 231
BshFI	GGCC	4 cut(s)	8, 178, 1110, 1808
BsiHKCI	CYCGRG	1 cut(s)	1925
BsiSI	CCGG	3 cut(s)	51, 65, 170
BslFI	GGGAC	2 cut(s)	82, 1907
BslI	CCNNNNNNNGG	5 cut(s)	169, 235, 1382, 1612, 1770
BsmAI	GTCTC	2 cut(s)	1837, 1922
BsmFI	GGGAC	2 cut(s)	82, 1907
BsmI	GAATGC	3 cut(s)	1053, 1754, 1838
BsnI	GGCC	4 cut(s)	8, 178, 1110, 1808
BsoBI	CYCGRG	1 cut(s)	1925
Bsp119I	TTCGAA	1 cut(s)	1459
Bsp120I	GGGCCC	1 cut(s)	1806
Bsp1286I	GDGCHC	2 cut(s)	1668, 1810
Bsp143I	GATC	8 cut(s)	689, 838, 1034, 1132, 1234, 1405, 1447, 1541
BspACI	CCGC	8 cut(s)	5, 37, 54, 173, 229, 488, 548, 632
BspANI	GGCC	4 cut(s)	8, 178, 1110, 1808
BspCNI	CTCAG	2 cut(s)	1341, 1486
BspFNI	CGCG	2 cut(s)	225, 231
BspLI	GGNNCC	5 cut(s)	70, 71, 177, 1808, 1948
BspMAI	CTGCAG	2 cut(s)	435, 846
BspOI	GCTAGC	1 cut(s)	408
BspPI	GGATC	5 cut(s)	697, 1042, 1242, 1400, 1442
BspT104I	TTCGAA	1 cut(s)	1459
BspTI	CTTAAG	1 cut(s)	542
BsrDI	GCAATG	1 cut(s)	1515
BsrFI	RCCGGY	1 cut(s)	169
BsrI	ACTGG	1 cut(s)	1585
BssAI	RCCGGY	1 cut(s)	169
BssECI	CCNNGG	5 cut(s)	9, 65, 336, 1810, 1950
BssMI	GATC	8 cut(s)	689, 838, 1034, 1132, 1234, 1405, 1447, 1541
BssNI	GRCGYC	2 cut(s)	200, 1261
BssSI	CACGAG	1 cut(s)	1932
BssT1I	CCWWGG	4 cut(s)	9, 336, 1810, 1950
Bst2BI	CACGAG	1 cut(s)	1932
Bst2UI	CCWGG	1 cut(s)	1319
Bst4CI	ACNGT	2 cut(s)	777, 990
Bst6I	CTCTTC	2 cut(s)	954, 1008
BstACI	GRCGYC	2 cut(s)	200, 1261
BstAFI	CTTAAG	1 cut(s)	542
BstBAI	YACGTR	1 cut(s)	1118
BstBI	TTCGAA	1 cut(s)	1459
BstC8I	GCNNGC	7 cut(s)	243, 297, 406, 630, 708, 1491, 1875
BstDEI	CTNAG	7 cut(s)	60, 234, 1328, 1494, 1538, 1843, 1860
BstENI	CCTNNNNNAGG	1 cut(s)	1380
BstFNI	CGCG	2 cut(s)	225, 231
BstHHI	GCGC	2 cut(s)	233, 301
BstKTI	GATC	8 cut(s)	692, 841, 1037, 1135, 1237, 1408, 1450, 1544
BstMAI	GTCTC	2 cut(s)	1837, 1922
BstMBI	GATC	8 cut(s)	689, 838, 1034, 1132, 1234, 1405, 1447, 1541
BstMWI	GCNNNNNNNGC	3 cut(s)	547, 1267, 1844
BstNI	CCWGG	1 cut(s)	1319
BstNSI	RCATGY	2 cut(s)	662, 710
BstSCI	CCNGG	2 cut(s)	64, 1317
BstSFI	CTRYAG	8 cut(s)	431, 693, 765, 773, 842, 1271, 1644, 1822
BstSLI	GKGCMC	1 cut(s)	1810
BstSNI	TACGTA	1 cut(s)	1118
BstUI	CGCG	2 cut(s)	225, 231
BstV1I	GCAGC	3 cut(s)	637, 1903, 1949
BstV2I	GAAGAC	1 cut(s)	1738
BstX2I	RGATCY	5 cut(s)	689, 838, 1034, 1234, 1447
BstYI	RGATCY	5 cut(s)	689, 838, 1034, 1234, 1447
BsuRI	GGCC	4 cut(s)	8, 178, 1110, 1808
BtsI	GCAGTG	2 cut(s)	577, 1075
BtsIMutI	CAGTG	4 cut(s)	577, 1075, 1781, 1895
Cac8I	GCNNGC	7 cut(s)	243, 297, 406, 630, 708, 1491, 1875
CaiI	CAGNNNCTG	1 cut(s)	1897
CfoI	GCGC	2 cut(s)	233, 301
Cfr10I	RCCGGY	1 cut(s)	169
Cfr13I	GGNCC	7 cut(s)	13, 69, 176, 998, 1722, 1806, 1807
CseI	GACGC	4 cut(s)	208, 214, 665, 1250
Csp6I	GTAC	5 cut(s)	57, 448, 943, 1419, 1911
CviAII	CATG	8 cut(s)	290, 413, 653, 659, 682, 707, 868, 1564
CviQI	GTAC	5 cut(s)	57, 448, 943, 1419, 1911
DdeI	CTNAG	7 cut(s)	60, 234, 1328, 1494, 1538, 1843, 1860
DpnI	GATC	8 cut(s)	691, 840, 1036, 1134, 1236, 1407, 1449, 1543
DpnII	GATC	8 cut(s)	689, 838, 1034, 1132, 1234, 1405, 1447, 1541
DraI	TTTAAA	1 cut(s)	1230
DraIII	CACNNNGTG	2 cut(s)	572, 1770
DrdI	GACNNNNNNGTC	1 cut(s)	205
DseDI	GACNNNNNNGTC	1 cut(s)	205
EaeI	YGGCCR	1 cut(s)	6
Eam1104I	CTCTTC	2 cut(s)	954, 1008
EarI	CTCTTC	2 cut(s)	954, 1008
Eco105I	TACGTA	1 cut(s)	1118
Eco130I	CCWWGG	4 cut(s)	9, 336, 1810, 1950
Eco24I	GRGCYC	2 cut(s)	1668, 1810
Eco47I	GGWCC	4 cut(s)	13, 69, 998, 1722
Eco57I	CTGAAG	6 cut(s)	837, 881, 978, 1401, 1437, 1836
Eco88I	CYCGRG	1 cut(s)	1925
EcoNI	CCTNNNNNAGG	1 cut(s)	1380
EcoO109I	RGGNCCY	3 cut(s)	69, 176, 998
EcoRII	CCWGG	1 cut(s)	1317
EcoT14I	CCWWGG	4 cut(s)	9, 336, 1810, 1950
EcoT22I	ATGCAT	2 cut(s)	712, 1517
EcoT38I	GRGCYC	2 cut(s)	1668, 1810
ErhI	CCWWGG	4 cut(s)	9, 336, 1810, 1950
FaeI	CATG	8 cut(s)	293, 416, 656, 662, 685, 710, 871, 1567
FaqI	GGGAC	2 cut(s)	82, 1907
FatI	CATG	8 cut(s)	289, 412, 652, 658, 681, 706, 867, 1563
FauI	CCCGC	1 cut(s)	166
FauNDI	CATATG	1 cut(s)	577
FbaI	TGATCA	1 cut(s)	1132
Fnu4HI	GCNGC	5 cut(s)	6, 548, 626, 1892, 1938
FriOI	GRGCYC	2 cut(s)	1668, 1810
Fsp4HI	GCNGC	5 cut(s)	6, 548, 626, 1892, 1938
FspBI	CTAG	6 cut(s)	252, 405, 1038, 1128, 1502, 1716
FspI	TGCGCA	1 cut(s)	300
GlaI	GCGC	2 cut(s)	232, 300
GluI	GCNGC	5 cut(s)	6, 548, 626, 1892, 1938
HaeIII	GGCC	4 cut(s)	8, 178, 1110, 1808
HapII	CCGG	3 cut(s)	51, 65, 170
HgaI	GACGC	4 cut(s)	208, 214, 665, 1250
HhaI	GCGC	2 cut(s)	233, 301
Hin1I	GRCGYC	2 cut(s)	200, 1261
Hin1II	CATG	8 cut(s)	293, 416, 656, 662, 685, 710, 871, 1567
Hin6I	GCGC	2 cut(s)	231, 299
HinP1I	GCGC	2 cut(s)	231, 299
HincII	GTYRAC	1 cut(s)	910
HindII	GTYRAC	1 cut(s)	910
HindIII	AAGCTT	1 cut(s)	1187
HinfI	GANTC	8 cut(s)	140, 322, 880, 1018, 1306, 1364, 1596, 1856
HpaII	CCGG	3 cut(s)	51, 65, 170
HphI	GGTGA	3 cut(s)	604, 889, 940
Hpy166II	GTNNAC	3 cut(s)	910, 1295, 1867
Hpy188I	TCNGA	7 cut(s)	1013, 1369, 1381, 1675, 1722, 1855, 1917
Hpy188III	TCNNGA	7 cut(s)	326, 395, 796, 859, 1220, 1409, 1480
Hpy8I	GTNNAC	3 cut(s)	910, 1295, 1867
Hpy99I	CGWCG	2 cut(s)	110, 202
HpyCH4III	ACNGT	2 cut(s)	777, 990
HpyCH4IV	ACGT	1 cut(s)	1117
HpyF10VI	GCNNNNNNNGC	3 cut(s)	547, 1267, 1844
HpyF3I	CTNAG	7 cut(s)	60, 234, 1328, 1494, 1538, 1843, 1860
HpySE526I	ACGT	1 cut(s)	1117
Hsp92I	GRCGYC	2 cut(s)	200, 1261
Hsp92II	CATG	8 cut(s)	293, 416, 656, 662, 685, 710, 871, 1567
HspAI	GCGC	2 cut(s)	231, 299
KflI	GGGWCCC	1 cut(s)	69
Ksp22I	TGATCA	1 cut(s)	1132
Kzo9I	GATC	8 cut(s)	689, 838, 1034, 1132, 1234, 1405, 1447, 1541
LmnI	GCTCC	3 cut(s)	238, 413, 538
Lsp1109I	GCAGC	3 cut(s)	637, 1903, 1949
LweI	GCATC	7 cut(s)	310, 460, 487, 514, 674, 1856, 1894
MaeI	CTAG	6 cut(s)	252, 405, 1038, 1128, 1502, 1716
MaeII	ACGT	1 cut(s)	1117
MaeIII	GTNAC	3 cut(s)	645, 677, 1814
MalI	GATC	8 cut(s)	691, 840, 1036, 1134, 1236, 1407, 1449, 1543
MboI	GATC	8 cut(s)	689, 838, 1034, 1132, 1234, 1405, 1447, 1541
MfeI	CAATTG	1 cut(s)	716
MflI	RGATCY	5 cut(s)	689, 838, 1034, 1234, 1447
MhlI	GDGCHC	2 cut(s)	1668, 1810
MlyI	GAGTC	5 cut(s)	134, 331, 1300, 1373, 1850
MmeI	TCCRAC	4 cut(s)	667, 1024, 1377, 1700
Mph1103I	ATGCAT	2 cut(s)	712, 1517
MseI	TTAA	6 cut(s)	444, 543, 783, 984, 1209, 1229
MslI	CAYNNNNRTG	1 cut(s)	657
MspA1I	CMGCKG	1 cut(s)	1821
MspCI	CTTAAG	1 cut(s)	542
MspI	CCGG	3 cut(s)	51, 65, 170
MspR9I	CCNGG	2 cut(s)	66, 1319
MunI	CAATTG	1 cut(s)	716
Mva1269I	GAATGC	3 cut(s)	1053, 1754, 1838
MvaI	CCWGG	1 cut(s)	1319
MvnI	CGCG	2 cut(s)	225, 231
MwoI	GCNNNNNNNGC	3 cut(s)	547, 1267, 1844
NciI	CCSGG	1 cut(s)	66
NdeI	CATATG	1 cut(s)	577
NdeII	GATC	8 cut(s)	689, 838, 1034, 1132, 1234, 1405, 1447, 1541
NheI	GCTAGC	1 cut(s)	404
NlaIII	CATG	8 cut(s)	293, 416, 656, 662, 685, 710, 871, 1567
NlaIV	GGNNCC	5 cut(s)	70, 71, 177, 1808, 1948
NmeAIII	GCCGAG	1 cut(s)	214
NmuCI	GTSAC	3 cut(s)	645, 677, 1814
NsbI	TGCGCA	1 cut(s)	300
NsiI	ATGCAT	2 cut(s)	712, 1517
NspI	RCATGY	2 cut(s)	662, 710
NspV	TTCGAA	1 cut(s)	1459
PaeI	GCATGC	1 cut(s)	710
PciI	ACATGT	1 cut(s)	658
PcsI	WCGNNNNNNNCGW	1 cut(s)	206
PctI	GAATGC	3 cut(s)	1053, 1754, 1838
PfeI	GAWTC	3 cut(s)	880, 1018, 1596
PflFI	GACNNNGTC	1 cut(s)	791
PflMI	CCANNNNNTGG	1 cut(s)	1770
PkrI	GCNGC	5 cut(s)	7, 549, 627, 1893, 1939
PleI	GAGTC	5 cut(s)	134, 330, 1300, 1372, 1850
PpsI	GAGTC	5 cut(s)	134, 330, 1300, 1372, 1850
Ppu21I	YACGTR	1 cut(s)	1118
PpuMI	RGGWCCY	2 cut(s)	69, 998
PscI	ACATGT	1 cut(s)	658
PshBI	ATTAAT	1 cut(s)	1209
Psp5II	RGGWCCY	2 cut(s)	69, 998
Psp6I	CCWGG	1 cut(s)	1317
PspGI	CCWGG	1 cut(s)	1317
PspN4I	GGNNCC	5 cut(s)	70, 71, 177, 1808, 1948
PspOMI	GGGCCC	1 cut(s)	1806
PspPI	GGNCC	7 cut(s)	13, 69, 176, 998, 1722, 1806, 1807
PspPPI	RGGWCCY	2 cut(s)	69, 998
PstI	CTGCAG	2 cut(s)	435, 846
PstNI	CAGNNNCTG	1 cut(s)	1897
PsuI	RGATCY	5 cut(s)	689, 838, 1034, 1234, 1447
PsyI	GACNNNGTC	1 cut(s)	791
PvuII	CAGCTG	1 cut(s)	1821
RsaI	GTAC	5 cut(s)	58, 449, 944, 1420, 1912
RsaNI	GTAC	5 cut(s)	57, 448, 943, 1419, 1911
RseI	CAYNNNNRTG	1 cut(s)	657
SaqAI	TTAA	6 cut(s)	444, 543, 783, 984, 1209, 1229
SatI	GCNGC	5 cut(s)	6, 548, 626, 1892, 1938
Sau3AI	GATC	8 cut(s)	689, 838, 1034, 1132, 1234, 1405, 1447, 1541
Sau96I	GGNCC	7 cut(s)	13, 69, 176, 998, 1722, 1806, 1807
ScaI	AGTACT	2 cut(s)	449, 944
SchI	GAGTC	5 cut(s)	134, 331, 1300, 1373, 1850
ScrFI	CCNGG	2 cut(s)	66, 1319
SduI	GDGCHC	2 cut(s)	1668, 1810
SfaNI	GCATC	7 cut(s)	310, 460, 487, 514, 674, 1856, 1894
SfcI	CTRYAG	8 cut(s)	431, 693, 765, 773, 842, 1271, 1644, 1822
SfuI	TTCGAA	1 cut(s)	1459
SinI	GGWCC	4 cut(s)	13, 69, 998, 1722
SmiMI	CAYNNNNRTG	1 cut(s)	657
SmlI	CTYRAG	1 cut(s)	542
SmoI	CTYRAG	1 cut(s)	542
SnaBI	TACGTA	1 cut(s)	1118
SphI	GCATGC	1 cut(s)	710
SsiI	CCGC	8 cut(s)	5, 37, 54, 173, 229, 488, 548, 632
SspMI	CTAG	6 cut(s)	252, 405, 1038, 1128, 1502, 1716
StyD4I	CCNGG	2 cut(s)	64, 1317
StyI	CCWWGG	4 cut(s)	9, 336, 1810, 1950
TaaI	ACNGT	2 cut(s)	777, 990
TaiI	ACGT	1 cut(s)	1120
TaqI	TCGA	4 cut(s)	130, 209, 325, 1459
TatI	WGTACW	2 cut(s)	447, 942
TauI	GCSGC	2 cut(s)	8, 550
TfiI	GAWTC	3 cut(s)	880, 1018, 1596
Tru1I	TTAA	6 cut(s)	444, 543, 783, 984, 1209, 1229
Tru9I	TTAA	6 cut(s)	444, 543, 783, 984, 1209, 1229
TscAI	CASTG	4 cut(s)	577, 1075, 1788, 1902
TseFI	GTSAC	3 cut(s)	645, 677, 1814
TseI	GCWGC	3 cut(s)	625, 1891, 1937
Tsp45I	GTSAC	3 cut(s)	645, 677, 1814
TspDTI	ATGAA	8 cut(s)	395, 575, 641, 1010, 1118, 1271, 1644, 1653
TspGWI	ACGGA	1 cut(s)	1254
TspRI	CASTG	4 cut(s)	577, 1075, 1788, 1902
Tth111I	GACNNNGTC	1 cut(s)	791
Van91I	CCANNNNNTGG	1 cut(s)	1770
Vha464I	CTTAAG	1 cut(s)	542
VpaK11BI	GGWCC	4 cut(s)	13, 69, 998, 1722
VspI	ATTAAT	1 cut(s)	1209
XagI	CCTNNNNNAGG	1 cut(s)	1380
XapI	RAATTY	2 cut(s)	390, 1705
XceI	RCATGY	2 cut(s)	662, 710
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1430
XspI	CTAG	6 cut(s)	252, 405, 1038, 1128, 1502, 1716
ZrmI	AGTACT	2 cut(s)	449, 944
Zsp2I	ATGCAT	2 cut(s)	712, 1517

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.