Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007659

Physical Location & Seq

Genomic Coordinates Forward (+)

16486044 .. 16495450

Gene Structure 9407 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1189300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2163 bp

ATGGCTCACGACGCCAATCCACTTTCTTCTGCTCTGATCTGCTCATTGGAAGCTAAGACGAACGTCCCAACGCTCCTTCCGATGGATTCAGCGGCAGCTCAGCGTCGCCTTAAAGCAATCAACCGCCACCTCGCCACCGGCGTCGACTCCGAGTCCCCTCTCCGGCCCAATCCCACCACCGCCGGCGAGTTCTTTTCCGAGCATGGGTACAGTGTGGTGCTTCCAGAGAAATTAGACACAGGAAAGTGGAATGTATACAGATCTGCACGCTCCCCAATGAAGCTTGTTAGTAGATTCCCCGATCATCCTGAAATTGGTACACTGCATGATAATTTTGTGCATTCAGTTGAAACTTTCCGAGATTACAAATATTTGGGCACACGAATTCGCGTTGATGGAACAGTTGGAGATTACAAGTGGATGACATACGGAGAAGCAGGTACTGCTCGGTCAGGAATAGGTTCTGGCCTAATTTATAATGGAATACCAAAGGGATCTTCAATTGGATTGTATTTTATTAACAGACCTGAGTGGCTCATTGTTGATCATGCCTGCTCTGCGTATTCGTATATATCAGTTCCTTTGTATGACACTCTAGGTCCAGATGCTGTCAAGTACATTGTAAATCATGCTGTTATGCAAGTTATCTTTTGTGTGCCTCAAACATTGAATAGTTTATTGAGCTTCTTGGCGGATATTCCAACCGTACGCCTGATTGTGGTTGTTGGGGGGATGGATGATCAAATGCCATCTCTTCCGTCATCGGCTGGAGTGAAGGTTGTAACATATTCAAAATTGCTGTCTCAGGGCTCTAGTAGTCTTCAGCCTTTTTTCCCTCCAAAACCAGAGGATGTTGCAACAATATGCTACACAAGTGGTACAACAGGCACTCCAAAGGGAGCTGTATTGACTCATGGAAACTTGATTGCAAATGTTGCTGGGACCACGATTGCTATCAAATTCTACCCGTCCGACATCTACATATCATATCTTCCTTTGGCACACATTTATGAGCGGGTTAACCAGGTCATGACGGCATACTTTGGAGTTGCTGTTGGATTCTACCAAGGGGATAATTTAAAATTAATGGACGATATGGCAGCTCTAAGACCTACCATCTTCAGCAGTGTTCCTAGGCTGTATAACAGAATATATGCGGGTATTACAAATGCGGTCAAGACATCCGGTGTACTGAGGGAGAGACTATTTAATGCTGCTTACAATGCCAAGAAGCAGGCACTATTAAGTGGAAAAAATCCATCCCCCATGTGGGATAGATTGGTATTCAATAAGATAAAGGCTAAGCTAGGAGGACGAGTTCGTTTTATGACATCAGGTGCTTCACCCTTGTCACCTGATGTAATGCAGTTTTTGAAGATATGCTTTAGTGGTGAAGTGGCTGAAGGGTATGGAATGACTGAGACTTCTTGTGTTATTAGTGCTATGGATGGGGATGACAAAGTATGTGGGCATGTTGGCTCTCCTAATCCAGCTTGTGAAATAAAGCTTGTTGATGTTCCAGAAATGAACTATACATCTGATGATCAACCATATCCTCGTGGTGAAATCTGTGTGAGGGGTCCTATTATATTCCAAGGATATCACAAAGATGAAGTGCAGACGAGGGAAGTGGTCGACGAAGATGGATGGCTTCATACTGGAGACATTGGGTTGTGGTTACCTGGAGGTCGTCTGAAAATTATTGATAGGAAGAAGAACATTTTCAAGTTGGCACAGGGAGAGTATATAGCTCCAGAGAAAGTCGAGAATGTCTATGCCAAGTGCAAGTTTATTGCCCAGTGCTTTGTTTATGGCGACAGCTTTAATTCTTCTTTGGTAGCGATTGTCGCAGTCGACCCGGATGCATTGAAAGCATGGGCTGATTCTGAAGGCATTAAGTTTGAAGATGTAGGGAAACTATGCAATGACCCAAGAGCAAGAGCAGCTGTATTGGCCGATATGGATGCTGCTGGCAGGGAAGCTCAGTTGAGAGGTTTCGAATTTGTAAAAGCCGTGACACTGGTGCTTGAACCGTTCACATTGGAGAATGGATTGCTCACTCCGACATTTAAGATCAAGAGACCTCAAGCAAAGGAGTACTATGCAAAAGCAATATCGGCCATGTATTCAGAGCTCGTCGCTTCTGAATCCTCCCCTCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001101 GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010193 GO:0015645 GO:0016020 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0032787 GO:0042221 GO:0042493 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0050896 GO:0071704 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00061 ko00071 ko01100 ko01212 ko02024 ko03320 ko04146 ko04216 ko04714 ko04920 map00061 map00071 map01100 map01212 map02024 map03320 map04146 map04216 map04714 map04920

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

721

Amino Acids

78.91

Weight (kDa)

7.17

Isoelectric Point (pI)

29.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	132 - 535	6.8e-86	AMP-binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007659)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05970
fragaria_vesca	FvH4_4g15110 FvH4_6g05940
malus_domestica	MD13G1188800.v1.1 MD16G1189300.v1.1
prunus_persica	Prupe.1G155800_v2.0.a1
pyrus_communis	pycom13g16310 pycom16g15930
rosa_chinensis	RchiOBHm_Chr4g0416751
rosa_laevigata	RLG00000007955
rosa_multiflora	Rmu_sc0028652.1_g000009 Rmu_sc0028670.1_g000001 Rmu_sc0028671.1_g000001
rosa_roxburghii	Rroxscaffold_5G00360620
rosa_rugosa	Rorug04G0144200 Rorug04G0144300 Rorug04G0144400 Rorug04G0144400 Rorug04G0144500
rosa_samantha	Rh4AG207100 Rh4BG204300 Rh4CG218100 Rh4DG204200
rosa_wichuraiana	Rw4G017520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	477
Acc36I	ACCTGC	1 cut(s)	428
AccBSI	CCGCTC	1 cut(s)	1015
AccI	GTMKAC	4 cut(s)	144, 255, 1635, 1854
AccII	CGCG	1 cut(s)	390
AciI	CCGC	7 cut(s)	92, 124, 180, 692, 1015, 1157, 1172
AclWI	GGATC	1 cut(s)	502
AcoI	YGGCCR	2 cut(s)	1953, 2118
AcsI	RAATTY	3 cut(s)	384, 959, 2000
AcuI	CTGAAG	4 cut(s)	806, 1105, 1422, 1908
AcyI	GRCGYC	2 cut(s)	12, 141
AfaI	GTAC	8 cut(s)	209, 319, 442, 617, 708, 880, 1191, 2099
AfiI	CCNNNNNNNGG	7 cut(s)	82, 162, 204, 314, 718, 1269, 1270
AhdI	GACNNNNNGTC	1 cut(s)	151
AjnI	CCWGG	2 cut(s)	1023, 1681
AjuI	GAANNNNNNNTTGG	2 cut(s)	481, 513
AloI	GAACNNNNNNTCC	2 cut(s)	1302, 1334
Alw21I	GWGCWC	1 cut(s)	2136
Alw26I	GTCTC	5 cut(s)	807, 1195, 1415, 1656, 2074
AlwI	GGATC	1 cut(s)	502
AlwNI	CAGNNNCTG	2 cut(s)	443, 608
AoxI	GGCC	4 cut(s)	164, 466, 1953, 2118
ApeKI	GCWGC	5 cut(s)	95, 1100, 1214, 1943, 1967
ApoI	RAATTY	3 cut(s)	384, 959, 2000
ArsI	GACNNNNNNTTYG	2 cut(s)	785, 817
AseI	ATTAAT	1 cut(s)	1085
Asp700I	GAANNNNTTC	2 cut(s)	460, 1721
AspA2I	CCTAGG	1 cut(s)	1133
AspS9I	GGNCC	4 cut(s)	165, 599, 942, 1580
AsuC2I	CCSGG	1 cut(s)	1859
AsuHPI	GGTGA	4 cut(s)	1335, 1344, 1403, 1574
AsuII	TTCGAA	1 cut(s)	1998
AvaII	GGWCC	3 cut(s)	599, 942, 1580
AvrII	CCTAGG	1 cut(s)	1133
BaeGI	GKGCMC	1 cut(s)	380
BanII	GRGCYC	2 cut(s)	812, 2136
BauI	CACGAG	1 cut(s)	1557
BbsI	GAAGAC	1 cut(s)	812
Bbv12I	GWGCWC	1 cut(s)	2136
BbvI	GCAGC	5 cut(s)	107, 1112, 1201, 1954, 1955
BccI	CCATC	9 cut(s)	76, 389, 727, 757, 1124, 1267, 1442, 1637, 1641
BceAI	ACGGC	2 cut(s)	1050, 1997
BcgI	CGANNNNNNTGC	4 cut(s)	1844, 1878, 1946, 1980
BciT130I	CCWGG	2 cut(s)	1025, 1683
BclI	TGATCA	3 cut(s)	544, 739, 1543
BcnI	CCSGG	1 cut(s)	1859
BcoDI	GTCTC	5 cut(s)	807, 1195, 1415, 1656, 2074
BfaI	CTAG	4 cut(s)	596, 813, 1134, 1307
BfuAI	ACCTGC	1 cut(s)	428
BglII	AGATCT	1 cut(s)	260
BisI	GCNGC	6 cut(s)	93, 96, 1101, 1215, 1944, 1968
BlnI	CCTAGG	1 cut(s)	1133
BlpI	GCTNAGC	2 cut(s)	99, 1302
BlsI	GCNGC	6 cut(s)	94, 97, 1102, 1216, 1945, 1969
BmcAI	AGTACT	1 cut(s)	2099
Bme1390I	CCNGG	3 cut(s)	1025, 1683, 1859
Bme18I	GGWCC	3 cut(s)	599, 942, 1580
BmeRI	GACNNNNNGTC	1 cut(s)	151
BmgT120I	GGNCC	4 cut(s)	165, 599, 942, 1580
BmiI	GGNNCC	2 cut(s)	943, 1581
BmrFI	CCNGG	3 cut(s)	1025, 1683, 1859
BmrI	ACTGGG	1 cut(s)	1792
BmsI	GCATC	3 cut(s)	595, 1852, 1954
BmuI	ACTGGG	1 cut(s)	1792
BoxI	GACNNNNGTC	1 cut(s)	62
BpiI	GAAGAC	1 cut(s)	812
BpmI	CTGGAG	4 cut(s)	789, 1680, 1704, 1737
Bpu1102I	GCTNAGC	2 cut(s)	99, 1302
Bpu14I	TTCGAA	1 cut(s)	1998
BpuEI	CTTGAG	1 cut(s)	2070
BpuMI	CCSGG	1 cut(s)	1859
BsaBI	GATNNNNATC	1 cut(s)	953
BsaHI	GRCGYC	2 cut(s)	12, 141
BsaI	GGTCTC	1 cut(s)	2074
BsaJI	CCNNGG	3 cut(s)	1066, 1133, 1594
BsaWI	WCCGGW	1 cut(s)	1184
BsaXI	ACNNNNNCTCC	8 cut(s)	762, 792, 874, 904, 1038, 1068, 1302, 1332
Bsc4I	CCNNNNNNNGG	7 cut(s)	82, 162, 204, 314, 718, 1269, 1270
Bse118I	RCCGGY	2 cut(s)	137, 182
Bse1I	ACTGG	3 cut(s)	1663, 1798, 2025
Bse3DI	GCAATG	1 cut(s)	1930
Bse8I	GATNNNNATC	1 cut(s)	953
BseBI	CCWGG	2 cut(s)	1025, 1683
BseDI	CCNNGG	3 cut(s)	1066, 1133, 1594
BseJI	GATNNNNATC	1 cut(s)	953
BseLI	CCNNNNNNNGG	7 cut(s)	82, 162, 204, 314, 718, 1269, 1270
BseMI	GCAATG	1 cut(s)	1930
BseMII	CTCAG	6 cut(s)	113, 519, 818, 1184, 1410, 1997
BseNI	ACTGG	3 cut(s)	1663, 1798, 2025
BseSI	GKGCMC	1 cut(s)	380
BseXI	GCAGC	5 cut(s)	107, 1112, 1201, 1954, 1955
BseYI	CCCAGC	1 cut(s)	938
BsgI	GTGCAG	2 cut(s)	249, 1637
Bsh1236I	CGCG	1 cut(s)	390
BshFI	GGCC	4 cut(s)	166, 468, 1955, 2120
BsiHKAI	GWGCWC	1 cut(s)	2136
BsiSI	CCGG	5 cut(s)	138, 163, 183, 1185, 1859
BsiWI	CGTACG	1 cut(s)	706
BslFI	GGGAC	3 cut(s)	50, 139, 955
BslI	CCNNNNNNNGG	7 cut(s)	82, 162, 204, 314, 718, 1269, 1270
BsmAI	GTCTC	5 cut(s)	807, 1195, 1415, 1656, 2074
BsmFI	GGGAC	3 cut(s)	50, 139, 955
BsmI	GAATGC	1 cut(s)	340
BsnI	GGCC	4 cut(s)	166, 468, 1955, 2120
Bso31I	GGTCTC	1 cut(s)	2074
Bsp119I	TTCGAA	1 cut(s)	1998
Bsp1286I	GDGCHC	3 cut(s)	380, 812, 2136
Bsp143I	GATC	8 cut(s)	36, 260, 301, 494, 544, 739, 1543, 2073
Bsp1720I	GCTNAGC	2 cut(s)	99, 1302
BspACI	CCGC	7 cut(s)	92, 124, 180, 692, 1015, 1157, 1172
BspANI	GGCC	4 cut(s)	166, 468, 1955, 2120
BspCNI	CTCAG	6 cut(s)	112, 520, 817, 1185, 1411, 1996
BspFNI	CGCG	1 cut(s)	390
BspHI	TCATGA	1 cut(s)	1029
BspLI	GGNNCC	2 cut(s)	943, 1581
BspMI	ACCTGC	1 cut(s)	428
BspPI	GGATC	1 cut(s)	502
BspT104I	TTCGAA	1 cut(s)	1998
BspTNI	GGTCTC	1 cut(s)	2074
BsrBI	CCGCTC	1 cut(s)	1015
BsrDI	GCAATG	1 cut(s)	1930
BsrFI	RCCGGY	2 cut(s)	137, 182
BsrI	ACTGG	3 cut(s)	1663, 1798, 2025
BssAI	RCCGGY	2 cut(s)	137, 182
BssECI	CCNNGG	3 cut(s)	1066, 1133, 1594
BssMI	GATC	8 cut(s)	36, 260, 301, 494, 544, 739, 1543, 2073
BssNAI	GTATAC	1 cut(s)	256
BssNI	GRCGYC	2 cut(s)	12, 141
BssSI	CACGAG	1 cut(s)	1557
BssT1I	CCWWGG	3 cut(s)	1066, 1133, 1594
Bst1107I	GTATAC	1 cut(s)	256
Bst2BI	CACGAG	1 cut(s)	1557
Bst2UI	CCWGG	2 cut(s)	1025, 1683
Bst4CI	ACNGT	4 cut(s)	212, 403, 706, 2034
Bst6I	CTCTTC	1 cut(s)	759
BstACI	GRCGYC	2 cut(s)	12, 141
BstAPI	GCANNNNNTGC	2 cut(s)	443, 935
BstBI	TTCGAA	1 cut(s)	1998
BstC8I	GCNNGC	5 cut(s)	184, 268, 553, 1236, 1972
BstDEI	CTNAG	9 cut(s)	54, 99, 528, 804, 1106, 1193, 1302, 1419, 1983
BstEII	GGTNACC	1 cut(s)	1677
BstFNI	CGCG	1 cut(s)	390
BstKTI	GATC	8 cut(s)	39, 263, 304, 497, 547, 742, 1546, 2076
BstMAI	GTCTC	5 cut(s)	807, 1195, 1415, 1656, 2074
BstMBI	GATC	8 cut(s)	36, 260, 301, 494, 544, 739, 1543, 2073
BstNI	CCWGG	2 cut(s)	1025, 1683
BstNSI	RCATGY	1 cut(s)	1475
BstPAI	GACNNNNGTC	1 cut(s)	62
BstPI	GGTNACC	1 cut(s)	1677
BstSCI	CCNGG	3 cut(s)	1023, 1681, 1857
BstSLI	GKGCMC	1 cut(s)	380
BstUI	CGCG	1 cut(s)	390
BstV1I	GCAGC	5 cut(s)	107, 1112, 1201, 1954, 1955
BstV2I	GAAGAC	1 cut(s)	812
BstX2I	RGATCY	2 cut(s)	260, 494
BstYI	RGATCY	2 cut(s)	260, 494
BstZ17I	GTATAC	1 cut(s)	256
BsuRI	GGCC	4 cut(s)	166, 468, 1955, 2120
BtsI	GCAGTG	2 cut(s)	320, 1132
BtsIMutI	CAGTG	5 cut(s)	217, 320, 1132, 1805, 2018
BveI	ACCTGC	1 cut(s)	428
Cac8I	GCNNGC	5 cut(s)	184, 268, 553, 1236, 1972
CaiI	CAGNNNCTG	2 cut(s)	443, 608
CciI	TCATGA	1 cut(s)	1029
Cfr10I	RCCGGY	2 cut(s)	137, 182
Cfr13I	GGNCC	4 cut(s)	165, 599, 942, 1580
CseI	GACGC	3 cut(s)	20, 92, 130
CsiI	ACCWGGT	1 cut(s)	1023
Csp6I	GTAC	8 cut(s)	208, 318, 441, 616, 707, 879, 1190, 2098
CspCI	CAANNNNNGTGG	2 cut(s)	1447, 1482
CviQI	GTAC	8 cut(s)	208, 318, 441, 616, 707, 879, 1190, 2098
DdeI	CTNAG	9 cut(s)	54, 99, 528, 804, 1106, 1193, 1302, 1419, 1983
DpnI	GATC	8 cut(s)	38, 262, 303, 496, 546, 741, 1545, 2075
DpnII	GATC	8 cut(s)	36, 260, 301, 494, 544, 739, 1543, 2073
DraI	TTTAAA	1 cut(s)	1080
DriI	GACNNNNNGTC	1 cut(s)	151
EaeI	YGGCCR	2 cut(s)	1953, 2118
Eam1104I	CTCTTC	1 cut(s)	759
Eam1105I	GACNNNNNGTC	1 cut(s)	151
EarI	CTCTTC	1 cut(s)	759
EciI	GGCGGA	1 cut(s)	707
Ecl136II	GAGCTC	1 cut(s)	2134
Eco130I	CCWWGG	3 cut(s)	1066, 1133, 1594
Eco24I	GRGCYC	2 cut(s)	812, 2136
Eco31I	GGTCTC	1 cut(s)	2074
Eco32I	GATATC	1 cut(s)	1601
Eco47I	GGWCC	3 cut(s)	599, 942, 1580
Eco53kI	GAGCTC	1 cut(s)	2134
Eco57I	CTGAAG	4 cut(s)	806, 1105, 1422, 1908
Eco91I	GGTNACC	1 cut(s)	1677
EcoICRI	GAGCTC	1 cut(s)	2134
EcoO109I	RGGNCCY	1 cut(s)	1580
EcoO65I	GGTNACC	1 cut(s)	1677
EcoRI	GAATTC	1 cut(s)	384
EcoRII	CCWGG	2 cut(s)	1023, 1681
EcoRV	GATATC	1 cut(s)	1601
EcoT14I	CCWWGG	3 cut(s)	1066, 1133, 1594
EcoT22I	ATGCAT	1 cut(s)	1867
EcoT38I	GRGCYC	2 cut(s)	812, 2136
ErhI	CCWWGG	3 cut(s)	1066, 1133, 1594
FalI	AAGNNNNNCTT	2 cut(s)	1367, 1399
FaqI	GGGAC	3 cut(s)	50, 139, 955
FauI	CCCGC	2 cut(s)	1008, 1150
FbaI	TGATCA	3 cut(s)	544, 739, 1543
FblI	GTMKAC	4 cut(s)	144, 255, 1635, 1854
Fnu4HI	GCNGC	6 cut(s)	93, 96, 1101, 1215, 1944, 1968
FriOI	GRGCYC	2 cut(s)	812, 2136
Fsp4HI	GCNGC	6 cut(s)	93, 96, 1101, 1215, 1944, 1968
FspBI	CTAG	4 cut(s)	596, 813, 1134, 1307
GluI	GCNGC	6 cut(s)	93, 96, 1101, 1215, 1944, 1968
GsaI	CCCAGC	1 cut(s)	942
GsuI	CTGGAG	4 cut(s)	789, 1680, 1704, 1737
HaeIII	GGCC	4 cut(s)	166, 468, 1955, 2120
HapII	CCGG	5 cut(s)	138, 163, 183, 1185, 1859
HgaI	GACGC	3 cut(s)	20, 92, 130
Hin1I	GRCGYC	2 cut(s)	12, 141
HincII	GTYRAC	4 cut(s)	145, 1021, 1636, 1855
HindII	GTYRAC	4 cut(s)	145, 1021, 1636, 1855
HindIII	AAGCTT	2 cut(s)	281, 1505
HinfI	GANTC	8 cut(s)	86, 146, 152, 294, 910, 1059, 1883, 2147
HpaI	GTTAAC	1 cut(s)	1021
HpaII	CCGG	5 cut(s)	138, 163, 183, 1185, 1859
HphI	GGTGA	4 cut(s)	1335, 1344, 1403, 1574
Hpy166II	GTNNAC	8 cut(s)	145, 256, 320, 1021, 1190, 1636, 1855, 2037
Hpy8I	GTNNAC	8 cut(s)	145, 256, 320, 1021, 1190, 1636, 1855, 2037
Hpy99I	CGWCG	5 cut(s)	14, 108, 146, 1640, 2141
HpyAV	CCTTC	4 cut(s)	86, 769, 1397, 1883
HpyCH4III	ACNGT	4 cut(s)	212, 403, 706, 2034
HpyCH4IV	ACGT	1 cut(s)	63
HpyF3I	CTNAG	9 cut(s)	54, 99, 528, 804, 1106, 1193, 1302, 1419, 1983
HpySE526I	ACGT	1 cut(s)	63
Hsp92I	GRCGYC	2 cut(s)	12, 141
KroI	GCCGGC	1 cut(s)	182
KroNI	GCCGGC	1 cut(s)	184
Ksp22I	TGATCA	3 cut(s)	544, 739, 1543
KspAI	GTTAAC	1 cut(s)	1021
Kzo9I	GATC	8 cut(s)	36, 260, 301, 494, 544, 739, 1543, 2073
LmnI	GCTCC	4 cut(s)	78, 275, 899, 1756
Lsp1109I	GCAGC	5 cut(s)	107, 1112, 1201, 1954, 1955
LweI	GCATC	3 cut(s)	595, 1852, 1954
MabI	ACCWGGT	1 cut(s)	1023
MaeI	CTAG	4 cut(s)	596, 813, 1134, 1307
MaeII	ACGT	1 cut(s)	63
MaeIII	GTNAC	4 cut(s)	781, 1350, 1677, 2014
MalI	GATC	8 cut(s)	38, 262, 303, 496, 546, 741, 1545, 2075
MbiI	CCGCTC	1 cut(s)	1015
MboI	GATC	8 cut(s)	36, 260, 301, 494, 544, 739, 1543, 2073
MfeI	CAATTG	1 cut(s)	501
MflI	RGATCY	2 cut(s)	260, 494
MhlI	GDGCHC	3 cut(s)	380, 812, 2136
MlyI	GAGTC	3 cut(s)	140, 161, 904
MmeI	TCCRAC	5 cut(s)	385, 725, 996, 1036, 2087
Mph1103I	ATGCAT	1 cut(s)	1867
MreI	CGCCGGCG	1 cut(s)	182
MroNI	GCCGGC	1 cut(s)	182
MroXI	GAANNNNTTC	2 cut(s)	460, 1721
MslI	CAYNNNNRTG	2 cut(s)	1008, 1608
MspA1I	CMGCKG	2 cut(s)	92, 1946
MspI	CCGG	5 cut(s)	138, 163, 183, 1185, 1859
MspR9I	CCNGG	3 cut(s)	1025, 1683, 1859
MunI	CAATTG	1 cut(s)	501
Mva1269I	GAATGC	1 cut(s)	340
MvaI	CCWGG	2 cut(s)	1025, 1683
MvnI	CGCG	1 cut(s)	390
NaeI	GCCGGC	1 cut(s)	184
NciI	CCSGG	1 cut(s)	1859
NdeII	GATC	8 cut(s)	36, 260, 301, 494, 544, 739, 1543, 2073
NgoMIV	GCCGGC	1 cut(s)	182
NlaIV	GGNNCC	2 cut(s)	943, 1581
NmuCI	GTSAC	2 cut(s)	1350, 2014
NsiI	ATGCAT	1 cut(s)	1867
NspI	RCATGY	1 cut(s)	1475
NspV	TTCGAA	1 cut(s)	1998
PagI	TCATGA	1 cut(s)	1029
PcsI	WCGNNNNNNNCGW	2 cut(s)	77, 138
PctI	GAATGC	1 cut(s)	340
PdiI	GCCGGC	1 cut(s)	184
PdmI	GAANNNNTTC	2 cut(s)	460, 1721
PfeI	GAWTC	5 cut(s)	86, 294, 1059, 1883, 2147
Pfl23II	CGTACG	1 cut(s)	706
PkrI	GCNGC	6 cut(s)	94, 97, 1102, 1216, 1945, 1969
PleI	GAGTC	3 cut(s)	140, 160, 904
PpsI	GAGTC	3 cut(s)	140, 160, 904
PpuMI	RGGWCCY	1 cut(s)	1580
PshAI	GACNNNNGTC	1 cut(s)	62
PshBI	ATTAAT	1 cut(s)	1085
PsiI	TTATAA	1 cut(s)	477
Psp124BI	GAGCTC	1 cut(s)	2136
Psp5II	RGGWCCY	1 cut(s)	1580
Psp6I	CCWGG	2 cut(s)	1023, 1681
PspEI	GGTNACC	1 cut(s)	1677
PspFI	CCCAGC	1 cut(s)	938
PspGI	CCWGG	2 cut(s)	1023, 1681
PspLI	CGTACG	1 cut(s)	706
PspN4I	GGNNCC	2 cut(s)	943, 1581
PspPI	GGNCC	4 cut(s)	165, 599, 942, 1580
PspPPI	RGGWCCY	1 cut(s)	1580
PstNI	CAGNNNCTG	2 cut(s)	443, 608
PsuI	RGATCY	2 cut(s)	260, 494
PvuII	CAGCTG	1 cut(s)	1946
RsaI	GTAC	8 cut(s)	209, 319, 442, 617, 708, 880, 1191, 2099
RsaNI	GTAC	8 cut(s)	208, 318, 441, 616, 707, 879, 1190, 2098
RseI	CAYNNNNRTG	2 cut(s)	1008, 1608
SacI	GAGCTC	1 cut(s)	2136
SalI	GTCGAC	3 cut(s)	143, 1634, 1853
SatI	GCNGC	6 cut(s)	93, 96, 1101, 1215, 1944, 1968
Sau3AI	GATC	8 cut(s)	36, 260, 301, 494, 544, 739, 1543, 2073
Sau96I	GGNCC	4 cut(s)	165, 599, 942, 1580
ScaI	AGTACT	1 cut(s)	2099
SchI	GAGTC	3 cut(s)	140, 161, 904
ScrFI	CCNGG	3 cut(s)	1025, 1683, 1859
SduI	GDGCHC	3 cut(s)	380, 812, 2136
SexAI	ACCWGGT	1 cut(s)	1023
SfaNI	GCATC	3 cut(s)	595, 1852, 1954
SfuI	TTCGAA	1 cut(s)	1998
SgrAI	CRCCGGYG	2 cut(s)	137, 182
SinI	GGWCC	3 cut(s)	599, 942, 1580
SmiMI	CAYNNNNRTG	2 cut(s)	1008, 1608
SmlI	CTYRAG	1 cut(s)	2085
SmoI	CTYRAG	1 cut(s)	2085
SsiI	CCGC	7 cut(s)	92, 124, 180, 692, 1015, 1157, 1172
SspI	AATATT	1 cut(s)	371
SspMI	CTAG	4 cut(s)	596, 813, 1134, 1307
SstI	GAGCTC	1 cut(s)	2136
StyD4I	CCNGG	3 cut(s)	1023, 1681, 1857
StyI	CCWWGG	3 cut(s)	1066, 1133, 1594
TaaI	ACNGT	4 cut(s)	212, 403, 706, 2034
TaiI	ACGT	1 cut(s)	66
TaqI	TCGA	6 cut(s)	144, 1635, 1764, 1854, 1998, 2158
TaqII	GACCGA	1 cut(s)	438
TatI	WGTACW	3 cut(s)	615, 1189, 2097
TauI	GCSGC	1 cut(s)	95
TfiI	GAWTC	5 cut(s)	86, 294, 1059, 1883, 2147
TscAI	CASTG	5 cut(s)	217, 327, 1132, 1805, 2025
TseFI	GTSAC	2 cut(s)	1350, 2014
TseI	GCWGC	5 cut(s)	95, 1100, 1214, 1943, 1967
Tsp45I	GTSAC	2 cut(s)	1350, 2014
TspDTI	ATGAA	4 cut(s)	293, 1541, 1626, 1643
TspGWI	ACGGA	2 cut(s)	444, 747
TspRI	CASTG	5 cut(s)	217, 327, 1132, 1805, 2025
VpaK11BI	GGWCC	3 cut(s)	599, 942, 1580
VspI	ATTAAT	1 cut(s)	1085
XapI	RAATTY	3 cut(s)	384, 959, 2000
XceI	RCATGY	1 cut(s)	1475
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1266
XmaJI	CCTAGG	1 cut(s)	1133
XmiI	GTMKAC	4 cut(s)	144, 255, 1635, 1854
XmnI	GAANNNNTTC	2 cut(s)	460, 1721
XspI	CTAG	4 cut(s)	596, 813, 1134, 1307
ZrmI	AGTACT	1 cut(s)	2099
Zsp2I	ATGCAT	1 cut(s)	1867

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.