Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0007659

Physical Location & Seq

Genomic Coordinates Reverse (-)

39713839 .. 39724282

Gene Structure 10444 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG204200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1983 bp

ATGGATTCCACTCCAGCTCAGCGTCGCCTCGAAGCCATTAACGGCCACCTCATCTCCGACGCCGACTCCCTCTCCATTCTCCGCCCCAACGCCACCGCCGGCGAGTTCGTTTCCGAGCAAGGGTACAGTGTGGTGCTTCCAGAGAAACTAGATACAGGAAAATGGAATGTATACAGATCTGCGCGTTCTCCTTTGAAGCTTGTTAGTAGATTCCCTGATCATCCCGAAATTGCTACGCTTCATGATAATTTTGTGCACTCAGTTGATACGTTCAGAGATTACAAATATTTGGGAACAAGAATTCGGGTCGATGGAACAGTTGGAGAATACAAATGGATGACGTTTGGAGAAGCAGATACTGCTCGGTCAGCAATAGGTTCTGGCCTCATATATCATGGGATACCAAAGGGATCTTGCATTGGGTTGTATTTTATAAACAGACCGGAGTGGATGATTGTTGATCATGCCTGCTCTGCATATTCTTACATATCAGTTCCTTTGTATGACACACTAGGTCCAGATGCTGTCAAATACATTGCAAATCATGCTGTTGTACAAGTTATATTTTGCGTTCCCGAAACATTGAATAGTATATTGAGCTTTCTCTCAGATATTCCAACTGTACGCCTCATTGTGGTTGTTGGAGGCATAGATGAACAAATACCATCCCTTCCGCCTTCAACGGGAGTGAAGGTTGTAACATATTCAACATTGCTCAATCAGGGCCGTAGTAGCATGCAGCCTTTCTGCCCACCAAAGTCGGATGATATTGCAACAATTTGCTACACAAGTGGCACCACTGGCACTCCAAAGGGAGCTGTATTGTCTCATGGAAACTTGATTGCAAGTGTTGCTGGGGCCACTTTTGGTATCCAGTTCTACCCCTCTGATGTTTACATATCATATCTTCCTTTGGCACACATATATGAGCGGGCTAACCAGGTCATGACGGTACATTTTGGAGTTGCTGTTGGATTCTACCAAGGGGATAATATGAAATTGATGGATGATATGGCTGCTCTAAGACCTACTATCTTCTGCAGTGTCCCTAGGCTGTATAACAGAATATATGCTGGAATCACGAATGCTGTAAAGACATCAGGTGTGCTGAGGGAGAGGCTATTTAATGCTGCTTACAATGCCAAGAAGAAGGCATTATTAAGTGGAAAAAATCCATCTCCCATGTGGGACAGATGCTTTGGGGGTGACGTAAATGAAGGGTATGGAATGACCGAGACTTCCTGTGTTATTAGTACTATGGACAGCGGTGACAACATATCTGGCCATGTTGGCTCTCCTAGTCCAGCTTGCGAAATCAAGCTGGTTGATGTACCAGAAATGAACTACACGTGTGATGATCAACCCTATCCACGCGGTGAAATCTGTGTCAGGGGTCCTATTATTTTTCAGGGCTATCACAAAGATGAAGTGCAGACGAGAGAAGTCATTGATGAAGATGGGTGGCTTCATACTGGAGACATTGGGTCATGGTTACCTGGTGGTCGTCTGAAAATTATTGATAGGAAGAAGAACATTTTCAAGTTGGCACAGGGAGAGTATATAGCTCCAGAAAAAATTGAGAATGTTTATGCCAAGTGCAAGTTTGTTGCCCAGTGCTTTGTACATGGGGACAGCCTAAATTCTTCTTTGGTAGCAATTGTCTCAGTTGACCCAGATATGTTGAAAGCATGGGCTGATTCTGAAGGCATAAAGTATGAAGATTTAGGGCGACTGTGTAATGATCCAAGAGCAAGGGCTGCTGTATTGGCTGATATGGATGCTATTGGCAGGGAAGCTCAGTTGAGAGGTTTTGAATTTGTAAAAGCCGTGACGCTGACGGTTGAACCGTTCACACTGGAGAATGGTCTACTCACTCCAACATTTAAGATCAAGAGACCTCAAGCAAAGGAATACTTTGCAAAAGCAATATCCAATATGTATTCGGAGCTTGCTACTTCTGATCCTGCCCCTTCAAAACTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001101 GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010193 GO:0015645 GO:0016020 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0032787 GO:0042221 GO:0042493 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0050896 GO:0071704 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00061 ko00071 ko01100 ko01212 ko02024 ko03320 ko04146 ko04216 ko04714 ko04920 map00061 map00071 map01100 map01212 map02024 map03320 map04146 map04216 map04714 map04920

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

660

Amino Acids

72.53

Weight (kDa)

5.92

Isoelectric Point (pI)

30.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	108 - 473	2.5e-77	AMP-binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007659)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05970
fragaria_vesca	FvH4_4g15110 FvH4_6g05940
malus_domestica	MD13G1188800.v1.1 MD16G1189300.v1.1
prunus_persica	Prupe.1G155800_v2.0.a1
pyrus_communis	pycom13g16310 pycom16g15930
rosa_chinensis	RchiOBHm_Chr4g0416751
rosa_laevigata	RLG00000007955
rosa_multiflora	Rmu_sc0028652.1_g000009 Rmu_sc0028670.1_g000001 Rmu_sc0028671.1_g000001
rosa_roxburghii	Rroxscaffold_5G00360620
rosa_rugosa	Rorug04G0144200 Rorug04G0144300 Rorug04G0144400 Rorug04G0144400 Rorug04G0144500
rosa_samantha	Rh4AG207100 Rh4BG204300 Rh4CG218100 Rh4DG204200
rosa_wichuraiana	Rw4G017520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	434
AccB1I	GGYRCC	1 cut(s)	794
AccBSI	CCGCTC	1 cut(s)	931
AccI	GTMKAC	2 cut(s)	171, 1867
AccII	CGCG	2 cut(s)	184, 1374
AciI	CCGC	6 cut(s)	82, 96, 674, 931, 1266, 1374
AclWI	GGATC	3 cut(s)	418, 1736, 1955
AcoI	YGGCCR	2 cut(s)	43, 1282
AcsI	RAATTY	3 cut(s)	300, 1639, 1814
AcuI	CTGAAG	1 cut(s)	1722
AcvI	CACGTG	1 cut(s)	1350
AcyI	GRCGYC	1 cut(s)	60
AdeI	CACNNNGTG	1 cut(s)	1376
AfaI	GTAC	7 cut(s)	125, 555, 624, 954, 1255, 1332, 1623
AfiI	CCNNNNNNNGG	3 cut(s)	120, 634, 683
AflIII	ACRYGT	2 cut(s)	1347, 1349
AgsI	TTSAA	9 cut(s)	196, 586, 681, 708, 1540, 1684, 1814, 1844, 1974
AhdI	GACNNNNNGTC	1 cut(s)	1483
AjnI	CCWGG	2 cut(s)	939, 1495
AluBI	AGCT	9 cut(s)	17, 199, 600, 818, 1307, 1321, 1565, 1796, 1948
AluI	AGCT	9 cut(s)	17, 199, 600, 818, 1307, 1321, 1565, 1796, 1948
Alw21I	GWGCWC	1 cut(s)	258
Alw26I	GTCTC	5 cut(s)	831, 1229, 1470, 1666, 1888
Alw44I	GTGCAC	1 cut(s)	254
AlwI	GGATC	3 cut(s)	418, 1736, 1955
AlwNI	CAGNNNCTG	2 cut(s)	359, 524
AoxI	GGCC	5 cut(s)	43, 382, 724, 858, 1282
ApaLI	GTGCAC	1 cut(s)	254
ApeKI	GCWGC	4 cut(s)	739, 1016, 1130, 1757
ApoI	RAATTY	3 cut(s)	300, 1639, 1814
ArsI	GACNNNNNNTTYG	2 cut(s)	1181, 1213
Asp700I	GAANNNNTTC	1 cut(s)	1535
AspA2I	CCTAGG	1 cut(s)	1049
AspLEI	GCGC	1 cut(s)	184
AspS9I	GGNCC	4 cut(s)	515, 724, 858, 1394
AsuHPI	GGTGA	3 cut(s)	1217, 1280, 1388
AvaII	GGWCC	2 cut(s)	515, 1394
AvrII	CCTAGG	1 cut(s)	1049
BaeGI	GKGCMC	1 cut(s)	258
BalI	TGGCCA	1 cut(s)	1284
BanI	GGYRCC	1 cut(s)	794
BbrPI	CACGTG	1 cut(s)	1350
Bbv12I	GWGCWC	1 cut(s)	258
BbvCI	CCTCAGC	1 cut(s)	1109
BbvI	GCAGC	4 cut(s)	751, 1003, 1117, 1744
BccI	CCATC	5 cut(s)	305, 673, 997, 1183, 1451
BceAI	ACGGC	3 cut(s)	58, 711, 1811
BciT130I	CCWGG	2 cut(s)	941, 1497
BciVI	GTATCC	2 cut(s)	393, 881
BclI	TGATCA	3 cut(s)	217, 460, 1357
BcoDI	GTCTC	5 cut(s)	831, 1229, 1470, 1666, 1888
BfaI	CTAG	4 cut(s)	149, 512, 1050, 1299
BfmI	CTRYAG	1 cut(s)	1039
BfuI	GTATCC	2 cut(s)	393, 881
BglI	GCCNNNNNGGC	1 cut(s)	1290
BglII	AGATCT	1 cut(s)	176
BisI	GCNGC	4 cut(s)	740, 1017, 1131, 1758
BlnI	CCTAGG	1 cut(s)	1049
BlpI	GCTNAGC	1 cut(s)	18
BlsI	GCNGC	4 cut(s)	741, 1018, 1132, 1759
BmcAI	AGTACT	1 cut(s)	1255
Bme1390I	CCNGG	2 cut(s)	941, 1497
Bme18I	GGWCC	2 cut(s)	515, 1394
BmeRI	GACNNNNNGTC	1 cut(s)	1483
BmgT120I	GGNCC	4 cut(s)	515, 724, 858, 1394
BmiI	GGNNCC	3 cut(s)	796, 859, 1395
BmrFI	CCNGG	2 cut(s)	941, 1497
BmrI	ACTGGG	1 cut(s)	1606
BmsI	GCATC	3 cut(s)	511, 1184, 1768
BmuI	ACTGGG	1 cut(s)	1606
BpmI	CTGGAG	3 cut(s)	1494, 1551, 1877
Bpu10I	CCTNAGC	1 cut(s)	1109
Bpu1102I	GCTNAGC	1 cut(s)	18
BpuEI	CTTGAG	1 cut(s)	1884
BsaAI	YACGTR	1 cut(s)	1350
BsaHI	GRCGYC	1 cut(s)	60
BsaI	GGTCTC	1 cut(s)	1888
BsaJI	CCNNGG	2 cut(s)	982, 1049
BsaWI	WCCGGW	1 cut(s)	442
Bsc4I	CCNNNNNNNGG	3 cut(s)	120, 634, 683
Bse118I	RCCGGY	1 cut(s)	98
Bse1I	ACTGG	5 cut(s)	805, 874, 1477, 1612, 1860
Bse3DI	GCAATG	2 cut(s)	534, 710
BseBI	CCWGG	2 cut(s)	941, 1497
BseDI	CCNNGG	2 cut(s)	982, 1049
BseGI	GGATG	7 cut(s)	220, 342, 456, 665, 769, 1012, 1783
BseLI	CCNNNNNNNGG	3 cut(s)	120, 634, 683
BseMI	GCAATG	2 cut(s)	534, 710
BseMII	CTCAG	6 cut(s)	32, 273, 621, 1100, 1677, 1811
BseNI	ACTGG	5 cut(s)	805, 874, 1477, 1612, 1860
BseSI	GKGCMC	1 cut(s)	258
BseXI	GCAGC	4 cut(s)	751, 1003, 1117, 1744
BseYI	CCCAGC	1 cut(s)	854
BsgI	GTGCAG	1 cut(s)	1451
Bsh1236I	CGCG	2 cut(s)	184, 1374
BshFI	GGCC	5 cut(s)	45, 384, 726, 860, 1284
BshNI	GGYRCC	1 cut(s)	794
BsiHKAI	GWGCWC	1 cut(s)	258
BsiSI	CCGG	2 cut(s)	99, 443
BslFI	GGGAC	3 cut(s)	1031, 1202, 1643
BslI	CCNNNNNNNGG	3 cut(s)	120, 634, 683
BsmAI	GTCTC	5 cut(s)	831, 1229, 1470, 1666, 1888
BsmFI	GGGAC	3 cut(s)	1031, 1202, 1643
BsmI	GAATGC	1 cut(s)	1090
BsnI	GGCC	5 cut(s)	45, 384, 726, 860, 1284
Bso31I	GGTCTC	1 cut(s)	1888
Bsp1286I	GDGCHC	1 cut(s)	258
Bsp1407I	TGTACA	2 cut(s)	553, 1621
Bsp143I	GATC	8 cut(s)	176, 217, 410, 460, 1357, 1741, 1887, 1960
Bsp1720I	GCTNAGC	1 cut(s)	18
BspACI	CCGC	6 cut(s)	82, 96, 674, 931, 1266, 1374
BspANI	GGCC	5 cut(s)	45, 384, 726, 860, 1284
BspCNI	CTCAG	6 cut(s)	31, 272, 620, 1101, 1676, 1810
BspFNI	CGCG	2 cut(s)	184, 1374
BspHI	TCATGA	2 cut(s)	241, 945
BspLI	GGNNCC	3 cut(s)	796, 859, 1395
BspMAI	CTGCAG	1 cut(s)	1043
BspPI	GGATC	3 cut(s)	418, 1736, 1955
BspT107I	GGYRCC	1 cut(s)	794
BspTNI	GGTCTC	1 cut(s)	1888
BsrBI	CCGCTC	1 cut(s)	931
BsrDI	GCAATG	2 cut(s)	534, 710
BsrFI	RCCGGY	1 cut(s)	98
BsrGI	TGTACA	2 cut(s)	553, 1621
BsrI	ACTGG	5 cut(s)	805, 874, 1477, 1612, 1860
BssAI	RCCGGY	1 cut(s)	98
BssECI	CCNNGG	2 cut(s)	982, 1049
BssMI	GATC	8 cut(s)	176, 217, 410, 460, 1357, 1741, 1887, 1960
BssNAI	GTATAC	1 cut(s)	172
BssNI	GRCGYC	1 cut(s)	60
BssT1I	CCWWGG	2 cut(s)	982, 1049
Bst1107I	GTATAC	1 cut(s)	172
Bst2UI	CCWGG	2 cut(s)	941, 1497
Bst4CI	ACNGT	7 cut(s)	128, 319, 622, 952, 1734, 1840, 1848
BstACI	GRCGYC	1 cut(s)	60
BstAPI	GCANNNNNTGC	4 cut(s)	359, 545, 851, 1757
BstAUI	TGTACA	2 cut(s)	553, 1621
BstBAI	YACGTR	1 cut(s)	1350
BstC8I	GCNNGC	6 cut(s)	100, 469, 737, 933, 1309, 1950
BstDEI	CTNAG	7 cut(s)	18, 259, 607, 1022, 1109, 1663, 1797
BstEII	GGTNACC	1 cut(s)	1491
BstF5I	GGATG	7 cut(s)	220, 342, 456, 665, 769, 1012, 1783
BstFNI	CGCG	2 cut(s)	184, 1374
BstHHI	GCGC	1 cut(s)	184
BstKTI	GATC	8 cut(s)	179, 220, 413, 463, 1360, 1744, 1890, 1963
BstMAI	GTCTC	5 cut(s)	831, 1229, 1470, 1666, 1888
BstMBI	GATC	8 cut(s)	176, 217, 410, 460, 1357, 1741, 1887, 1960
BstNI	CCWGG	2 cut(s)	941, 1497
BstNSI	RCATGY	1 cut(s)	739
BstPI	GGTNACC	1 cut(s)	1491
BstSCI	CCNGG	2 cut(s)	939, 1495
BstSFI	CTRYAG	1 cut(s)	1039
BstSLI	GKGCMC	1 cut(s)	258
BstUI	CGCG	2 cut(s)	184, 1374
BstV1I	GCAGC	4 cut(s)	751, 1003, 1117, 1744
BstX2I	RGATCY	2 cut(s)	176, 410
BstYI	RGATCY	2 cut(s)	176, 410
BstZ17I	GTATAC	1 cut(s)	172
BsuI	GTATCC	2 cut(s)	393, 881
BsuRI	GGCC	5 cut(s)	45, 384, 726, 860, 1284
BtsCI	GGATG	7 cut(s)	220, 342, 456, 665, 769, 1012, 1783
BtsI	GCAGTG	1 cut(s)	1048
BtsIMutI	CAGTG	5 cut(s)	133, 798, 1048, 1619, 1853
Cac8I	GCNNGC	6 cut(s)	100, 469, 737, 933, 1309, 1950
CaiI	CAGNNNCTG	2 cut(s)	359, 524
CciI	TCATGA	2 cut(s)	241, 945
CfoI	GCGC	1 cut(s)	184
Cfr10I	RCCGGY	1 cut(s)	98
Cfr13I	GGNCC	4 cut(s)	515, 724, 858, 1394
CseI	GACGC	3 cut(s)	11, 68, 1840
CsiI	ACCWGGT	2 cut(s)	939, 1495
Csp6I	GTAC	7 cut(s)	124, 554, 623, 953, 1254, 1331, 1622
CviQI	GTAC	7 cut(s)	124, 554, 623, 953, 1254, 1331, 1622
DdeI	CTNAG	7 cut(s)	18, 259, 607, 1022, 1109, 1663, 1797
DpnI	GATC	8 cut(s)	178, 219, 412, 462, 1359, 1743, 1889, 1962
DpnII	GATC	8 cut(s)	176, 217, 410, 460, 1357, 1741, 1887, 1960
DraIII	CACNNNGTG	1 cut(s)	1376
DriI	GACNNNNNGTC	1 cut(s)	1483
EaeI	YGGCCR	2 cut(s)	43, 1282
Eam1105I	GACNNNNNGTC	1 cut(s)	1483
EciI	GGCGGA	2 cut(s)	71, 663
Eco130I	CCWWGG	2 cut(s)	982, 1049
Eco31I	GGTCTC	1 cut(s)	1888
Eco47I	GGWCC	2 cut(s)	515, 1394
Eco57I	CTGAAG	1 cut(s)	1722
Eco72I	CACGTG	1 cut(s)	1350
Eco91I	GGTNACC	1 cut(s)	1491
EcoO109I	RGGNCCY	1 cut(s)	1394
EcoO65I	GGTNACC	1 cut(s)	1491
EcoRI	GAATTC	1 cut(s)	300
EcoRII	CCWGG	2 cut(s)	939, 1495
EcoT14I	CCWWGG	2 cut(s)	982, 1049
ErhI	CCWWGG	2 cut(s)	982, 1049
FalI	AAGNNNNNCTT	2 cut(s)	1898, 1930
FaqI	GGGAC	3 cut(s)	1031, 1202, 1643
FauI	CCCGC	1 cut(s)	924
FbaI	TGATCA	3 cut(s)	217, 460, 1357
FblI	GTMKAC	2 cut(s)	171, 1867
Fnu4HI	GCNGC	4 cut(s)	740, 1017, 1131, 1758
FokI	GGATG	7 cut(s)	207, 349, 463, 652, 776, 1019, 1790
Fsp4HI	GCNGC	4 cut(s)	740, 1017, 1131, 1758
FspBI	CTAG	4 cut(s)	149, 512, 1050, 1299
GlaI	GCGC	1 cut(s)	183
GluI	GCNGC	4 cut(s)	740, 1017, 1131, 1758
GsaI	CCCAGC	1 cut(s)	858
GsuI	CTGGAG	3 cut(s)	1494, 1551, 1877
HaeIII	GGCC	5 cut(s)	45, 384, 726, 860, 1284
HapII	CCGG	2 cut(s)	99, 443
HgaI	GACGC	3 cut(s)	11, 68, 1840
HhaI	GCGC	1 cut(s)	184
Hin1I	GRCGYC	1 cut(s)	60
Hin6I	GCGC	1 cut(s)	182
HinP1I	GCGC	1 cut(s)	182
HincII	GTYRAC	1 cut(s)	1669
HindII	GTYRAC	1 cut(s)	1669
HindIII	AAGCTT	1 cut(s)	197
HinfI	GANTC	6 cut(s)	5, 65, 210, 975, 1077, 1697
HpaII	CCGG	2 cut(s)	99, 443
HphI	GGTGA	3 cut(s)	1217, 1280, 1388
Hpy166II	GTNNAC	6 cut(s)	172, 256, 895, 1669, 1851, 1868
Hpy188III	TCNNGA	9 cut(s)	140, 224, 242, 518, 575, 946, 1081, 1568, 1891
Hpy8I	GTNNAC	6 cut(s)	172, 256, 895, 1669, 1851, 1868
Hpy99I	CGWCG	2 cut(s)	27, 62
HpyAV	CCTTC	7 cut(s)	680, 685, 687, 1144, 1211, 1697, 1980
HpyCH4III	ACNGT	7 cut(s)	128, 319, 622, 952, 1734, 1840, 1848
HpyCH4IV	ACGT	4 cut(s)	269, 341, 1209, 1349
HpyF3I	CTNAG	7 cut(s)	18, 259, 607, 1022, 1109, 1663, 1797
HpySE526I	ACGT	4 cut(s)	269, 341, 1209, 1349
Hsp92I	GRCGYC	1 cut(s)	60
HspAI	GCGC	1 cut(s)	182
KroI	GCCGGC	1 cut(s)	98
KroNI	GCCGGC	1 cut(s)	100
Ksp22I	TGATCA	3 cut(s)	217, 460, 1357
Kzo9I	GATC	8 cut(s)	176, 217, 410, 460, 1357, 1741, 1887, 1960
LmnI	GCTCC	3 cut(s)	815, 1570, 1945
Lsp1109I	GCAGC	4 cut(s)	751, 1003, 1117, 1744
LweI	GCATC	3 cut(s)	511, 1184, 1768
MabI	ACCWGGT	2 cut(s)	939, 1495
MaeI	CTAG	4 cut(s)	149, 512, 1050, 1299
MaeII	ACGT	4 cut(s)	269, 341, 1209, 1349
MaeIII	GTNAC	5 cut(s)	697, 1205, 1268, 1491, 1828
MalI	GATC	8 cut(s)	178, 219, 412, 462, 1359, 1743, 1889, 1962
MbiI	CCGCTC	1 cut(s)	931
MboI	GATC	8 cut(s)	176, 217, 410, 460, 1357, 1741, 1887, 1960
MboII	GAAGA	8 cut(s)	899, 1027, 1159, 1466, 1537, 1540, 1635, 1730
MfeI	CAATTG	1 cut(s)	1656
MflI	RGATCY	2 cut(s)	176, 410
MhlI	GDGCHC	1 cut(s)	258
MlsI	TGGCCA	1 cut(s)	1284
MluNI	TGGCCA	1 cut(s)	1284
MlyI	GAGTC	1 cut(s)	59
MmeI	TCCRAC	7 cut(s)	81, 301, 622, 641, 741, 952, 1901
Mox20I	TGGCCA	1 cut(s)	1284
MreI	CGCCGGCG	1 cut(s)	98
MroNI	GCCGGC	1 cut(s)	98
MroXI	GAANNNNTTC	1 cut(s)	1535
MscI	TGGCCA	1 cut(s)	1284
MseI	TTAA	4 cut(s)	39, 1125, 1160, 1884
MslI	CAYNNNNRTG	2 cut(s)	924, 1422
Msp20I	TGGCCA	1 cut(s)	1284
MspA1I	CMGCKG	1 cut(s)	1266
MspI	CCGG	2 cut(s)	99, 443
MspR9I	CCNGG	2 cut(s)	941, 1497
MunI	CAATTG	1 cut(s)	1656
Mva1269I	GAATGC	1 cut(s)	1090
MvaI	CCWGG	2 cut(s)	941, 1497
MvnI	CGCG	2 cut(s)	184, 1374
NaeI	GCCGGC	1 cut(s)	100
NdeII	GATC	8 cut(s)	176, 217, 410, 460, 1357, 1741, 1887, 1960
NgoMIV	GCCGGC	1 cut(s)	98
NlaIV	GGNNCC	3 cut(s)	796, 859, 1395
NmuCI	GTSAC	3 cut(s)	1205, 1268, 1828
NspI	RCATGY	1 cut(s)	739
PaeI	GCATGC	1 cut(s)	739
PagI	TCATGA	2 cut(s)	241, 945
PcsI	WCGNNNNNNNCGW	1 cut(s)	1844
PctI	GAATGC	1 cut(s)	1090
PdiI	GCCGGC	1 cut(s)	100
PdmI	GAANNNNTTC	1 cut(s)	1535
PfeI	GAWTC	5 cut(s)	5, 210, 975, 1077, 1697
PkrI	GCNGC	4 cut(s)	741, 1018, 1132, 1759
PleI	GAGTC	1 cut(s)	59
PmaCI	CACGTG	1 cut(s)	1350
PmlI	CACGTG	1 cut(s)	1350
PpsI	GAGTC	1 cut(s)	59
Ppu21I	YACGTR	1 cut(s)	1350
PpuMI	RGGWCCY	1 cut(s)	1394
PsiI	TTATAA	1 cut(s)	434
Psp5II	RGGWCCY	1 cut(s)	1394
Psp6I	CCWGG	2 cut(s)	939, 1495
PspCI	CACGTG	1 cut(s)	1350
PspEI	GGTNACC	1 cut(s)	1491
PspFI	CCCAGC	1 cut(s)	854
PspGI	CCWGG	2 cut(s)	939, 1495
PspN4I	GGNNCC	3 cut(s)	796, 859, 1395
PspPI	GGNCC	4 cut(s)	515, 724, 858, 1394
PspPPI	RGGWCCY	1 cut(s)	1394
PstI	CTGCAG	1 cut(s)	1043
PstNI	CAGNNNCTG	2 cut(s)	359, 524
PsuI	RGATCY	2 cut(s)	176, 410
RsaI	GTAC	7 cut(s)	125, 555, 624, 954, 1255, 1332, 1623
RsaNI	GTAC	7 cut(s)	124, 554, 623, 953, 1254, 1331, 1622
RseI	CAYNNNNRTG	2 cut(s)	924, 1422
SaqAI	TTAA	4 cut(s)	39, 1125, 1160, 1884
SatI	GCNGC	4 cut(s)	740, 1017, 1131, 1758
Sau3AI	GATC	8 cut(s)	176, 217, 410, 460, 1357, 1741, 1887, 1960
Sau96I	GGNCC	4 cut(s)	515, 724, 858, 1394
ScaI	AGTACT	1 cut(s)	1255
SchI	GAGTC	1 cut(s)	59
ScrFI	CCNGG	2 cut(s)	941, 1497
SduI	GDGCHC	1 cut(s)	258
SexAI	ACCWGGT	2 cut(s)	939, 1495
SfaNI	GCATC	3 cut(s)	511, 1184, 1768
SfcI	CTRYAG	1 cut(s)	1039
SgrAI	CRCCGGYG	1 cut(s)	98
SinI	GGWCC	2 cut(s)	515, 1394
SmiMI	CAYNNNNRTG	2 cut(s)	924, 1422
SmlI	CTYRAG	1 cut(s)	1899
SmoI	CTYRAG	1 cut(s)	1899
SphI	GCATGC	1 cut(s)	739
SsiI	CCGC	6 cut(s)	82, 96, 674, 931, 1266, 1374
SspI	AATATT	1 cut(s)	287
SspMI	CTAG	4 cut(s)	149, 512, 1050, 1299
StyD4I	CCNGG	2 cut(s)	939, 1495
StyI	CCWWGG	2 cut(s)	982, 1049
TaaI	ACNGT	7 cut(s)	128, 319, 622, 952, 1734, 1840, 1848
TaiI	ACGT	4 cut(s)	272, 344, 1212, 1352
TaqI	TCGA	2 cut(s)	30, 309
TaqII	GACCGA	2 cut(s)	354, 1247
TatI	WGTACW	3 cut(s)	553, 1253, 1621
TfiI	GAWTC	5 cut(s)	5, 210, 975, 1077, 1697
Tru1I	TTAA	4 cut(s)	39, 1125, 1160, 1884
Tru9I	TTAA	4 cut(s)	39, 1125, 1160, 1884
TscAI	CASTG	5 cut(s)	133, 805, 1048, 1619, 1860
TseFI	GTSAC	3 cut(s)	1205, 1268, 1828
TseI	GCWGC	4 cut(s)	739, 1016, 1130, 1757
Tsp45I	GTSAC	3 cut(s)	1205, 1268, 1828
TspDTI	ATGAA	9 cut(s)	230, 669, 1010, 1230, 1355, 1440, 1457, 1467, 1731
TspRI	CASTG	5 cut(s)	133, 805, 1048, 1619, 1860
VneI	GTGCAC	1 cut(s)	254
VpaK11BI	GGWCC	2 cut(s)	515, 1394
XapI	RAATTY	3 cut(s)	300, 1639, 1814
XceI	RCATGY	1 cut(s)	739
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1182
XmaJI	CCTAGG	1 cut(s)	1049
XmiI	GTMKAC	2 cut(s)	171, 1867
XmnI	GAANNNNTTC	1 cut(s)	1535
XspI	CTAG	4 cut(s)	149, 512, 1050, 1299
ZrmI	AGTACT	1 cut(s)	1255

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.