Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003761

Physical Location & Seq

Genomic Coordinates Reverse (-)

23925598 .. 23934651

Gene Structure 9054 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1232700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGGCTTCGGCTCAGGTACTGCCCAACTCGCGAAAGCAAGATCTTTTGGAAGCCGGAAAGCGCCGGCTGGAGGAGTTTCGGAAGAAAAAAGCAGCAGACCGAGCTAAGAAGGCTTCATCCACTAGCCAAATCGATGCTTCTGAAGTTAGCTCAAACGAGAAACAATCTCTAGAAACTGAACATGTACGAGTTACAGCCTCTGATGGTGCTGGTACATCTGATGGGCCCAGTAGTGCTTTTACTGAAACTTCTTCTGTATTATTGAACAATGATAGCAACGCGATTGATTTTTTGCAAAAAAATGAACAAGCTTCCGTGGATAATAATACACACAGCAGTCCTTCAACCTCTGAGTTTAATGCATATTCTGGAGATCAAGTACAGAAACATGCTGATCAAGAATATCTGAGATATGGTGTTTTAGGGTTTGGGGGACCACTGGCCGTTAATAATCGCCATGGGACTAATGGGATAAGTAATGATTTTGAAAAATATACTGGTGCCTTAGGTGGGACAACATCTGACCAGTCTTTTGCTCCACGCCCACAAGCAAATCAAGATTTTGCGGGCAATACAAGTCACTCCAGTTCTTATGGAAGGAATGAATTTCAGTCCAAAGAACATATTACATCTTTAATGGATTCTGGTTCTTCACATCCATCTGTTGCAAAAATCTCATCTCAGAACTCTGTCAGCACATTACTACAGAGTGAAGCCTCTAATGTCAGCATAGTGTCTGGTGGCCCTGCACCGTCTTCACTTTATGAAGACTTGGTTGAACCCAGTCCCAGCATAAGAGGATTTGCTTCTGAAGTTGGTAAAAACATCCGTGGTAGTAGTGAGGACTTAAGTGATTCTCTGAGTTATAAATTTGGGGAAGGAAAGCTTAGCAGCTTTGCCAGTAGCATTTCTAGTGGGCAGAGTGCACCAGTTCAAACATATGAATCCATGGGGGGTTTTGGATCTGATTCTAGAAGTTCCTCCAATCATGCATCATTGTATTCAGTTACACCTGAAACTAATTCTCGGAGATCTCGTCCATCCTTCCTTGATTCTCTTAATGTGTCTAAAACTTCTTCAGGCACTGTTTCTCAACAACCTGAACCAGAAGGGCCATTTATGTCCAACAGTTCTAAATCAAGTGGCATGAATTTTCTAGGTTCATCGCCTTTCCACAAGCCATCTATGGATGATGATACTGTGAGACCCTTTTTAAAGTTTGAAAGTGGTGCACCTCATGCATCTGAGCCTTCCATGAAGTCTTCACTTTCTCCTAATGCTTGGATGGACCAGCAGAGGCCAACAGTTGAGGGGAATAGCATGGAGAGAAAGCATGAATTTTATTCTCCTAATCAGAATGAAGATTTTTCTGCTTTGGAACAGCATATTGAAGATTTGACACAAGAAAAGTTCTCTTTGCAACGAGCACTTGAGGCTTCACGTGCTTTAGCAGAGTCATTGGCTGCTGAAAATTCTTCTTTGACAGAGAGTTATAACCAACAGAGAACTGTTGTTGATCAACTAAAATCTGACTTGGAAAATATACAGGAGGAAATCAAACACCAGCTGGTGGAACTTGATGCGGTCAGAAATGAATATGCAAATGCACAGCTAGAATGTAATGCCGCTGATGAACGTGCTAAGCTATTGGCATCGGAAGTTATCGGATTAGAGGAGAAGGCATTAAGACTAAGGTCCAGTGAGCTAAAGCTGGAGAGGCAATTGGAGAACTCACAGGCTGAAATCTTGTCGTACAAGAAAAGAATGTCCAGCCTAGAAAAAGATCGTTCAGATTTGCAGTCAACTATCAATGCTCTTCAGGAAGAGAAGAAGCTGTTGCAGTCTATGCTACGGAAAGCCTCCACAAGTGGAAATAAAGTTGATGTCAGCAAGAGTACTACTAACAACAAAGATGTGTCTACTTCTACAGAAGATCTAGAAGTTGGAGTTGTGGAAAATGAAGACGCTATTCCTGACACCTTAGGCCAGGAAGGGGGCGATGCCTCGAGTTTCCCAATGCTGCCTGAAAATGGACAGTCAACTTTTGACATTTCATCTGTGAATCTACCTCCCGATCAGATGCAAACAGTTGAAAACATCAGTACACTAATTTCCGAGTTAGCATTGGAGAAAGAAGAATTGATACAATCTTTGGCATCCGAGTCATCTCAATGTTCCAAGCTGAAGGAGCTGAACAATGAATTGTCTCGGAAACTTGAAGCCCAAACTCAGAGATTAGAGCTTTTGACAGCCCAAAGTATGGCTAATGAGAATACTCTCGTCAGACAACCCACTCCTGTTGATATGTCGTACAATGCTCCATATGCAGATGAAGGTGATGAGGTAGTGGAAAGAGTCTTGGGATGGATTATGAAGCTTTTCCCTGGAGGGCCATCAAAACGAAGAACTAGTAAGCTTCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009889 GO:0009908 GO:0009965 GO:0009987 GO:0010016 GO:0010026 GO:0010090 GO:0010091 GO:0010154 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032989 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051302 GO:0051781 GO:0060255 GO:0061458 GO:0065007 GO:0071840 GO:0080090 GO:0090558 GO:0090567 GO:0090626 GO:0090698 GO:0099402 GO:1903506 GO:1905392 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

808

Amino Acids

87.89

Weight (kDa)

4.91

Isoelectric Point (pI)

59.66

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003761)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23980
fragaria_vesca	FvH4_4g05311 FvH4_4g05311 FvH4_4g05311 FvH4_4g05311
malus_domestica	MD13G1227500.v1.1 MD16G1232700.v1.1
prunus_persica	Prupe.1G054700_v2.0.a1 Prupe.1G054700_v2.0.a1
pyrus_communis	pycom16g19470
rosa_chinensis	RchiOBHm_Chr4g0396341
rosa_laevigata	RLG00000009549
rosa_multiflora	Rmu_ssc0000234.1_g000016
rosa_roxburghii	Rroxscaffold_5G00341160 Rroxscaffold_7G00160200
rosa_rugosa	Rorug03G0349900 Rorug03G0350000 Rorug03G0350100 Rorug03G0350200 Rorug03G0350300
rosa_samantha	Rh1CG210400 Rh2BG187800 Rh3BG188400 Rh4BG066500 Rh4CG073300 Rh4DG063400 Rh5BG463000 Rh5CG484900 Rh6AG475600 Rh6BG312700 Rh6CG021900 Rh6DG022000 Rh7DG258100
rosa_wichuraiana	Rw4G005470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	867, 1494
AccB1I	GGYRCC	1 cut(s)	500
AccB7I	CCANNNNNTGG	2 cut(s)	1570, 2260
AccI	GTMKAC	1 cut(s)	1919
AccII	CGCG	2 cut(s)	31, 281
AciI	CCGC	3 cut(s)	566, 1583, 1626
AclWI	GGATC	1 cut(s)	970
AcoI	YGGCCR	1 cut(s)	441
AcsI	RAATTY	5 cut(s)	605, 869, 1150, 1337, 1471
AcuI	CTGAAG	5 cut(s)	162, 831, 1062, 1802, 2204
AcvI	CACGTG	1 cut(s)	1442
AfaI	GTAC	8 cut(s)	18, 186, 214, 381, 1754, 1897, 2104, 2312
AfiI	CCNNNNNNNGG	6 cut(s)	70, 510, 1570, 1993, 2030, 2260
AflII	CTTAAG	1 cut(s)	847
AflIII	ACRYGT	1 cut(s)	181
AgsI	TTSAA	9 cut(s)	265, 345, 488, 779, 935, 1223, 1391, 2093, 2219
AhlI	ACTAGT	1 cut(s)	2408
AjnI	CCWGG	2 cut(s)	1986, 2383
Alw21I	GWGCWC	3 cut(s)	928, 1234, 1429
Alw26I	GTCTC	2 cut(s)	1198, 2211
Alw44I	GTGCAC	2 cut(s)	924, 1230
AlwI	GGATC	1 cut(s)	970
AlwNI	CAGNNNCTG	3 cut(s)	19, 200, 1085
Ama87I	CYCGRG	1 cut(s)	2005
AoxI	GGCC	7 cut(s)	224, 441, 742, 1112, 1298, 1984, 2390
ApaI	GGGCCC	1 cut(s)	228
ApaLI	GTGCAC	2 cut(s)	924, 1230
ApeKI	GCWGC	4 cut(s)	92, 891, 1463, 2020
ApoI	RAATTY	5 cut(s)	605, 869, 1150, 1337, 1471
ArsI	GACNNNNNNTTYG	2 cut(s)	515, 547
Asp700I	GAANNNNTTC	1 cut(s)	2378
AspLEI	GCGC	1 cut(s)	63
AspS9I	GGNCC	8 cut(s)	224, 225, 434, 743, 1112, 1288, 1695, 2390
AsuHPI	GGTGA	1 cut(s)	2348
AvaI	CYCGRG	1 cut(s)	2005
AvaII	GGWCC	3 cut(s)	434, 1288, 1695
AxyI	CCTNAGG	2 cut(s)	505, 1981
BaeGI	GKGCMC	3 cut(s)	228, 928, 1234
BanI	GGYRCC	1 cut(s)	500
BanII	GRGCYC	1 cut(s)	228
BarI	GAAGNNNNNNTAC	2 cut(s)	2127, 2159
BbrPI	CACGTG	1 cut(s)	1442
BbsI	GAAGAC	4 cut(s)	747, 774, 1254, 1968
Bbv12I	GWGCWC	3 cut(s)	928, 1234, 1429
BbvI	GCAGC	4 cut(s)	104, 903, 1450, 2007
BccI	CCATC	8 cut(s)	197, 215, 667, 1048, 1189, 1279, 2358, 2401
BceAI	ACGGC	1 cut(s)	428
BciT130I	CCWGG	2 cut(s)	1988, 2385
BclI	TGATCA	2 cut(s)	394, 1516
BcoDI	GTCTC	2 cut(s)	1198, 2211
BcuI	ACTAGT	1 cut(s)	2408
BfaI	CTAG	9 cut(s)	123, 170, 912, 972, 1157, 1613, 1775, 1937, 2409
BfmI	CTRYAG	2 cut(s)	704, 1926
BfoI	RGCGCY	1 cut(s)	64
BfrI	CTTAAG	1 cut(s)	847
BglII	AGATCT	3 cut(s)	40, 1031, 1933
BisI	GCNGC	5 cut(s)	93, 892, 1464, 1626, 2021
BlpI	GCTNAGC	2 cut(s)	887, 1641
BlsI	GCNGC	5 cut(s)	94, 893, 1465, 1627, 2022
BmcAI	AGTACT	1 cut(s)	1897
Bme1390I	CCNGG	2 cut(s)	1988, 2385
Bme18I	GGWCC	3 cut(s)	434, 1288, 1695
BmeT110I	CYCGRG	1 cut(s)	2005
BmgT120I	GGNCC	8 cut(s)	224, 225, 434, 743, 1112, 1288, 1695, 2390
BmiI	GGNNCC	3 cut(s)	226, 435, 502
BmrFI	CCNGG	2 cut(s)	1988, 2385
BmrI	ACTGGG	2 cut(s)	222, 777
BmsI	GCATC	8 cut(s)	124, 1001, 1250, 1570, 1661, 1990, 2070, 2165
BmuI	ACTGGG	2 cut(s)	222, 777
BoxI	GACNNNNGTC	1 cut(s)	1693
BpiI	GAAGAC	4 cut(s)	747, 774, 1254, 1968
BpmI	CTGGAG	5 cut(s)	89, 390, 568, 1733, 2406
Bpu10I	CCTNAGC	1 cut(s)	12
Bpu1102I	GCTNAGC	2 cut(s)	887, 1641
BpuEI	CTTGAG	1 cut(s)	1451
Bsa29I	ATCGAT	1 cut(s)	132
BsaAI	YACGTR	1 cut(s)	1442
BsaI	GGTCTC	1 cut(s)	1198
BsaJI	CCNNGG	5 cut(s)	315, 457, 829, 948, 2383
BsaXI	ACNNNNNCTCC	4 cut(s)	363, 393, 1021, 1051
Bsc4I	CCNNNNNNNGG	6 cut(s)	70, 510, 1570, 1993, 2030, 2260
Bse118I	RCCGGY	1 cut(s)	63
Bse1I	ACTGG	9 cut(s)	228, 444, 502, 526, 585, 783, 900, 929, 1698
Bse21I	CCTNAGG	2 cut(s)	505, 1981
BseBI	CCWGG	2 cut(s)	1988, 2385
BseCI	ATCGAT	1 cut(s)	132
BseDI	CCNNGG	5 cut(s)	315, 457, 829, 948, 2383
BseGI	GGATG	8 cut(s)	116, 655, 825, 1040, 1195, 1290, 2156, 2369
BseLI	CCNNNNNNNGG	6 cut(s)	70, 510, 1570, 1993, 2030, 2260
BseMII	CTCAG	7 cut(s)	26, 342, 398, 695, 851, 1236, 2243
BseNI	ACTGG	9 cut(s)	228, 444, 502, 526, 585, 783, 900, 929, 1698
BseRI	GAGGAG	2 cut(s)	86, 1688
BseSI	GKGCMC	3 cut(s)	228, 928, 1234
BseXI	GCAGC	4 cut(s)	104, 903, 1450, 2007
BseYI	CCCAGC	1 cut(s)	788
BsgI	GTGCAG	1 cut(s)	732
Bsh1236I	CGCG	2 cut(s)	31, 281
BshFI	GGCC	7 cut(s)	226, 443, 744, 1114, 1300, 1986, 2392
BshNI	GGYRCC	1 cut(s)	500
BshVI	ATCGAT	1 cut(s)	132
BsiHKAI	GWGCWC	3 cut(s)	928, 1234, 1429
BsiHKCI	CYCGRG	1 cut(s)	2005
BsiSI	CCGG	2 cut(s)	54, 64
BslFI	GGGAC	4 cut(s)	447, 475, 526, 771
BslI	CCNNNNNNNGG	6 cut(s)	70, 510, 1570, 1993, 2030, 2260
BsmAI	GTCTC	2 cut(s)	1198, 2211
BsmFI	GGGAC	4 cut(s)	447, 475, 526, 771
BsnI	GGCC	7 cut(s)	226, 443, 744, 1114, 1300, 1986, 2392
Bso31I	GGTCTC	1 cut(s)	1198
BsoBI	CYCGRG	1 cut(s)	2005
Bsp120I	GGGCCC	1 cut(s)	224
Bsp1286I	GDGCHC	4 cut(s)	228, 928, 1234, 1429
Bsp143I	GATC	9 cut(s)	40, 373, 394, 962, 1031, 1516, 1783, 1933, 2074
Bsp1720I	GCTNAGC	2 cut(s)	887, 1641
Bsp19I	CCATGG	2 cut(s)	457, 948
Bsp68I	TCGCGA	1 cut(s)	31
BspACI	CCGC	3 cut(s)	566, 1583, 1626
BspANI	GGCC	7 cut(s)	226, 443, 744, 1114, 1300, 1986, 2392
BspCNI	CTCAG	7 cut(s)	25, 343, 399, 694, 852, 1237, 2242
BspDI	ATCGAT	1 cut(s)	132
BspFNI	CGCG	2 cut(s)	31, 281
BspLI	GGNNCC	3 cut(s)	226, 435, 502
BspPI	GGATC	1 cut(s)	970
BspQI	GCTCTTC	1 cut(s)	1821
BspT107I	GGYRCC	1 cut(s)	500
BspTI	CTTAAG	1 cut(s)	847
BspTNI	GGTCTC	1 cut(s)	1198
BsrFI	RCCGGY	1 cut(s)	63
BsrI	ACTGG	9 cut(s)	228, 444, 502, 526, 585, 783, 900, 929, 1698
BssAI	RCCGGY	1 cut(s)	63
BssECI	CCNNGG	5 cut(s)	315, 457, 829, 948, 2383
BssMI	GATC	9 cut(s)	40, 373, 394, 962, 1031, 1516, 1783, 1933, 2074
BssT1I	CCWWGG	2 cut(s)	457, 948
Bst2UI	CCWGG	2 cut(s)	1988, 2385
Bst4CI	ACNGT	8 cut(s)	753, 1087, 1130, 1201, 1306, 1510, 2037, 2089
Bst6I	CTCTTC	2 cut(s)	1818, 1821
BstAFI	CTTAAG	1 cut(s)	847
BstAPI	GCANNNNNTGC	2 cut(s)	1238, 1846
BstBAI	YACGTR	1 cut(s)	1442
BstC8I	GCNNGC	2 cut(s)	65, 568
BstDSI	CCRYGG	4 cut(s)	315, 457, 829, 948
BstF5I	GGATG	8 cut(s)	116, 655, 825, 1040, 1195, 1290, 2156, 2369
BstFNI	CGCG	2 cut(s)	31, 281
BstH2I	RGCGCY	1 cut(s)	64
BstHHI	GCGC	1 cut(s)	63
BstKTI	GATC	9 cut(s)	43, 376, 397, 965, 1034, 1519, 1786, 1936, 2077
BstMAI	GTCTC	2 cut(s)	1198, 2211
BstMBI	GATC	9 cut(s)	40, 373, 394, 962, 1031, 1516, 1783, 1933, 2074
BstNI	CCWGG	2 cut(s)	1988, 2385
BstNSI	RCATGY	2 cut(s)	185, 392
BstPAI	GACNNNNGTC	1 cut(s)	1693
BstSCI	CCNGG	2 cut(s)	1986, 2383
BstSFI	CTRYAG	2 cut(s)	704, 1926
BstSLI	GKGCMC	3 cut(s)	228, 928, 1234
BstUI	CGCG	2 cut(s)	31, 281
BstV1I	GCAGC	4 cut(s)	104, 903, 1450, 2007
BstV2I	GAAGAC	4 cut(s)	747, 774, 1254, 1968
BstX2I	RGATCY	4 cut(s)	40, 962, 1031, 1933
BstYI	RGATCY	4 cut(s)	40, 962, 1031, 1933
Bsu15I	ATCGAT	1 cut(s)	132
Bsu36I	CCTNAGG	2 cut(s)	505, 1981
BsuRI	GGCC	7 cut(s)	226, 443, 744, 1114, 1300, 1986, 2392
BsuTUI	ATCGAT	1 cut(s)	132
BtgI	CCRYGG	4 cut(s)	315, 457, 829, 948
BtgZI	GCGATG	2 cut(s)	1149, 2013
BtsCI	GGATG	8 cut(s)	116, 655, 825, 1040, 1195, 1290, 2156, 2369
BtsIMutI	CAGTG	3 cut(s)	437, 1083, 1705
BtuMI	TCGCGA	1 cut(s)	31
Cac8I	GCNNGC	2 cut(s)	65, 568
CaiI	CAGNNNCTG	3 cut(s)	19, 200, 1085
CfoI	GCGC	1 cut(s)	63
Cfr10I	RCCGGY	1 cut(s)	63
Cfr13I	GGNCC	8 cut(s)	224, 225, 434, 743, 1112, 1288, 1695, 2390
ClaI	ATCGAT	1 cut(s)	132
CseI	GACGC	1 cut(s)	1973
Csp6I	GTAC	8 cut(s)	17, 185, 213, 380, 1753, 1896, 2103, 2311
CspCI	CAANNNNNGTGG	2 cut(s)	2281, 2316
CviQI	GTAC	8 cut(s)	17, 185, 213, 380, 1753, 1896, 2103, 2311
DpnI	GATC	9 cut(s)	42, 375, 396, 964, 1033, 1518, 1785, 1935, 2076
DpnII	GATC	9 cut(s)	40, 373, 394, 962, 1031, 1516, 1783, 1933, 2074
DraI	TTTAAA	1 cut(s)	1215
EaeI	YGGCCR	1 cut(s)	441
Eam1104I	CTCTTC	2 cut(s)	1818, 1821
EarI	CTCTTC	2 cut(s)	1818, 1821
Eco130I	CCWWGG	2 cut(s)	457, 948
Eco24I	GRGCYC	1 cut(s)	228
Eco31I	GGTCTC	1 cut(s)	1198
Eco47I	GGWCC	3 cut(s)	434, 1288, 1695
Eco57I	CTGAAG	5 cut(s)	162, 831, 1062, 1802, 2204
Eco72I	CACGTG	1 cut(s)	1442
Eco81I	CCTNAGG	2 cut(s)	505, 1981
Eco88I	CYCGRG	1 cut(s)	2005
EcoRII	CCWGG	2 cut(s)	1986, 2383
EcoT14I	CCWWGG	2 cut(s)	457, 948
EcoT22I	ATGCAT	3 cut(s)	364, 994, 1243
EcoT38I	GRGCYC	1 cut(s)	228
ErhI	CCWWGG	2 cut(s)	457, 948
FalI	AAGNNNNNCTT	2 cut(s)	870, 902
FaqI	GGGAC	4 cut(s)	447, 475, 526, 771
FauI	CCCGC	1 cut(s)	559
FauNDI	CATATG	2 cut(s)	940, 2323
FbaI	TGATCA	2 cut(s)	394, 1516
FblI	GTMKAC	1 cut(s)	1919
Fnu4HI	GCNGC	5 cut(s)	93, 892, 1464, 1626, 2021
FokI	GGATG	8 cut(s)	103, 642, 812, 1027, 1202, 1297, 2143, 2376
FriOI	GRGCYC	1 cut(s)	228
Fsp4HI	GCNGC	5 cut(s)	93, 892, 1464, 1626, 2021
FspBI	CTAG	9 cut(s)	123, 170, 912, 972, 1157, 1613, 1775, 1937, 2409
GlaI	GCGC	1 cut(s)	62
GluI	GCNGC	5 cut(s)	93, 892, 1464, 1626, 2021
GsaI	CCCAGC	1 cut(s)	792
GsuI	CTGGAG	5 cut(s)	89, 390, 568, 1733, 2406
HaeII	RGCGCY	1 cut(s)	64
HaeIII	GGCC	7 cut(s)	226, 443, 744, 1114, 1300, 1986, 2392
HapII	CCGG	2 cut(s)	54, 64
HgaI	GACGC	1 cut(s)	1973
HhaI	GCGC	1 cut(s)	63
Hin6I	GCGC	1 cut(s)	61
HinP1I	GCGC	1 cut(s)	61
HincII	GTYRAC	2 cut(s)	1803, 2040
HindII	GTYRAC	2 cut(s)	1803, 2040
HindIII	AAGCTT	4 cut(s)	309, 884, 2375, 2414
HinfI	GANTC	9 cut(s)	641, 854, 944, 968, 1052, 1454, 2062, 2162, 2355
HpaII	CCGG	2 cut(s)	54, 64
HphI	GGTGA	1 cut(s)	2348
Hpy166II	GTNNAC	6 cut(s)	926, 1232, 1803, 1920, 2040, 2105
Hpy8I	GTNNAC	6 cut(s)	926, 1232, 1803, 1920, 2040, 2105
HpyCH4III	ACNGT	8 cut(s)	753, 1087, 1130, 1201, 1306, 1510, 2037, 2089
HpyCH4IV	ACGT	2 cut(s)	1441, 1636
HpySE526I	ACGT	2 cut(s)	1441, 1636
HspAI	GCGC	1 cut(s)	61
KroI	GCCGGC	1 cut(s)	63
KroNI	GCCGGC	1 cut(s)	65
Ksp22I	TGATCA	2 cut(s)	394, 1516
Kzo9I	GATC	9 cut(s)	40, 373, 394, 962, 1031, 1516, 1783, 1933, 2074
LguI	GCTCTTC	1 cut(s)	1821
LmnI	GCTCC	3 cut(s)	541, 2188, 2323
Lsp1109I	GCAGC	4 cut(s)	104, 903, 1450, 2007
LweI	GCATC	8 cut(s)	124, 1001, 1250, 1570, 1661, 1990, 2070, 2165
MaeI	CTAG	9 cut(s)	123, 170, 912, 972, 1157, 1613, 1775, 1937, 2409
MaeII	ACGT	2 cut(s)	1441, 1636
MaeIII	GTNAC	3 cut(s)	190, 578, 1006
MalI	GATC	9 cut(s)	42, 375, 396, 964, 1033, 1518, 1785, 1935, 2076
MboI	GATC	9 cut(s)	40, 373, 394, 962, 1031, 1516, 1783, 1933, 2074
MfeI	CAATTG	1 cut(s)	1721
MflI	RGATCY	4 cut(s)	40, 962, 1031, 1933
MhlI	GDGCHC	4 cut(s)	228, 928, 1234, 1429
MlyI	GAGTC	3 cut(s)	1463, 2171, 2364
MmeI	TCCRAC	2 cut(s)	1149, 1924
Mph1103I	ATGCAT	3 cut(s)	364, 994, 1243
MroNI	GCCGGC	1 cut(s)	63
MroXI	GAANNNNTTC	1 cut(s)	2378
MseI	TTAA	7 cut(s)	357, 447, 635, 848, 1059, 1214, 1685
MslI	CAYNNNNRTG	1 cut(s)	2170
MspA1I	CMGCKG	2 cut(s)	1567, 1628
MspCI	CTTAAG	1 cut(s)	847
MspI	CCGG	2 cut(s)	54, 64
MspR9I	CCNGG	2 cut(s)	1988, 2385
MunI	CAATTG	1 cut(s)	1721
MvaI	CCWGG	2 cut(s)	1988, 2385
MvnI	CGCG	2 cut(s)	31, 281
NaeI	GCCGGC	1 cut(s)	65
NcoI	CCATGG	2 cut(s)	457, 948
NdeI	CATATG	2 cut(s)	940, 2323
NdeII	GATC	9 cut(s)	40, 373, 394, 962, 1031, 1516, 1783, 1933, 2074
NgoMIV	GCCGGC	1 cut(s)	63
NlaIV	GGNNCC	3 cut(s)	226, 435, 502
NmuCI	GTSAC	1 cut(s)	578
NruI	TCGCGA	1 cut(s)	31
NsiI	ATGCAT	3 cut(s)	364, 994, 1243
NspI	RCATGY	2 cut(s)	185, 392
PaeR7I	CTCGAG	1 cut(s)	2005
PciI	ACATGT	1 cut(s)	181
PciSI	GCTCTTC	1 cut(s)	1821
PcsI	WCGNNNNNNNCGW	1 cut(s)	1033
PdiI	GCCGGC	1 cut(s)	65
PdmI	GAANNNNTTC	1 cut(s)	2378
PfeI	GAWTC	6 cut(s)	641, 854, 944, 968, 1052, 2062
PflMI	CCANNNNNTGG	2 cut(s)	1570, 2260
PkrI	GCNGC	5 cut(s)	94, 893, 1465, 1627, 2022
PleI	GAGTC	3 cut(s)	1462, 2170, 2363
PmaCI	CACGTG	1 cut(s)	1442
PmlI	CACGTG	1 cut(s)	1442
PpsI	GAGTC	3 cut(s)	1462, 2170, 2363
Ppu21I	YACGTR	1 cut(s)	1442
PscI	ACATGT	1 cut(s)	181
PshAI	GACNNNNGTC	1 cut(s)	1693
PsiI	TTATAA	2 cut(s)	867, 1494
Psp6I	CCWGG	2 cut(s)	1986, 2383
PspCI	CACGTG	1 cut(s)	1442
PspFI	CCCAGC	1 cut(s)	788
PspGI	CCWGG	2 cut(s)	1986, 2383
PspN4I	GGNNCC	3 cut(s)	226, 435, 502
PspOMI	GGGCCC	1 cut(s)	224
PspPI	GGNCC	8 cut(s)	224, 225, 434, 743, 1112, 1288, 1695, 2390
PspXI	VCTCGAGB	1 cut(s)	2005
PstNI	CAGNNNCTG	3 cut(s)	19, 200, 1085
PsuI	RGATCY	4 cut(s)	40, 962, 1031, 1933
PvuII	CAGCTG	1 cut(s)	1567
RruI	TCGCGA	1 cut(s)	31
RsaI	GTAC	8 cut(s)	18, 186, 214, 381, 1754, 1897, 2104, 2312
RsaNI	GTAC	8 cut(s)	17, 185, 213, 380, 1753, 1896, 2103, 2311
RseI	CAYNNNNRTG	1 cut(s)	2170
SapI	GCTCTTC	1 cut(s)	1821
SaqAI	TTAA	7 cut(s)	357, 447, 635, 848, 1059, 1214, 1685
SatI	GCNGC	5 cut(s)	93, 892, 1464, 1626, 2021
Sau3AI	GATC	9 cut(s)	40, 373, 394, 962, 1031, 1516, 1783, 1933, 2074
Sau96I	GGNCC	8 cut(s)	224, 225, 434, 743, 1112, 1288, 1695, 2390
ScaI	AGTACT	1 cut(s)	1897
SchI	GAGTC	3 cut(s)	1463, 2171, 2364
ScrFI	CCNGG	2 cut(s)	1988, 2385
SduI	GDGCHC	4 cut(s)	228, 928, 1234, 1429
SfaNI	GCATC	8 cut(s)	124, 1001, 1250, 1570, 1661, 1990, 2070, 2165
SfcI	CTRYAG	2 cut(s)	704, 1926
Sfr274I	CTCGAG	1 cut(s)	2005
SinI	GGWCC	3 cut(s)	434, 1288, 1695
SlaI	CTCGAG	1 cut(s)	2005
SmiMI	CAYNNNNRTG	1 cut(s)	2170
SmlI	CTYRAG	3 cut(s)	847, 1430, 2005
SmoI	CTYRAG	3 cut(s)	847, 1430, 2005
SpeI	ACTAGT	1 cut(s)	2408
SsiI	CCGC	3 cut(s)	566, 1583, 1626
SspMI	CTAG	9 cut(s)	123, 170, 912, 972, 1157, 1613, 1775, 1937, 2409
StyD4I	CCNGG	2 cut(s)	1986, 2383
StyI	CCWWGG	2 cut(s)	457, 948
TaaI	ACNGT	8 cut(s)	753, 1087, 1130, 1201, 1306, 1510, 2037, 2089
TaiI	ACGT	2 cut(s)	1444, 1639
TaqI	TCGA	2 cut(s)	132, 2006
TaqII	GACCGA	1 cut(s)	114
TatI	WGTACW	3 cut(s)	379, 1895, 2102
TauI	GCSGC	1 cut(s)	1628
TfiI	GAWTC	6 cut(s)	641, 854, 944, 968, 1052, 2062
Tru1I	TTAA	7 cut(s)	357, 447, 635, 848, 1059, 1214, 1685
Tru9I	TTAA	7 cut(s)	357, 447, 635, 848, 1059, 1214, 1685
TscAI	CASTG	3 cut(s)	444, 1090, 1705
TseFI	GTSAC	1 cut(s)	578
TseI	GCWGC	4 cut(s)	92, 891, 1463, 2020
Tsp45I	GTSAC	1 cut(s)	578
TspGWI	ACGGA	3 cut(s)	304, 818, 1867
TspRI	CASTG	3 cut(s)	444, 1090, 1705
Van91I	CCANNNNNTGG	2 cut(s)	1570, 2260
Vha464I	CTTAAG	1 cut(s)	847
VneI	GTGCAC	2 cut(s)	924, 1230
VpaK11BI	GGWCC	3 cut(s)	434, 1288, 1695
XapI	RAATTY	5 cut(s)	605, 869, 1150, 1337, 1471
XbaI	TCTAGA	3 cut(s)	169, 971, 1936
XceI	RCATGY	2 cut(s)	185, 392
XcmI	CCANNNNNNNNNTGG	1 cut(s)	464
XhoI	CTCGAG	1 cut(s)	2005
XmiI	GTMKAC	1 cut(s)	1919
XmnI	GAANNNNTTC	1 cut(s)	2378
XspI	CTAG	9 cut(s)	123, 170, 912, 972, 1157, 1613, 1775, 1937, 2409
ZrmI	AGTACT	1 cut(s)	1897
Zsp2I	ATGCAT	3 cut(s)	364, 994, 1243

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.