Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009804

Physical Location & Seq

Genomic Coordinates Forward (+)

25132004 .. 25137248

Gene Structure 5245 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1238200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGCTACGTACCTACACCACAATTATGGAGCAACGTAACAAGAAGATGAAGATGAAGATAATGATGATGCAGTGCGCATCATCATCGTTAATGTCATCTCGTGCTCTTGGTTTCTTCTTTGTTCTGTTACATGTTTGTGTTGCCTCTTCTGCGGAGGTTTCTGAAACCGAAATTCTCCTCAAGTTTAAGGGTTCCTTACAAAACACCGACGGAATAACCAATTGGAAGCCATCGACCAGTCCCTGCACCTCCCAAACGTCGAACTGGATGGGTGTTCGTTGCGATGGAAAGGGGAGAGTTTGGGCTCTGCAGCTTGAGAACATGGGGCTCAAGGGTGGAATCAATGTGGACTTGCTCAAGGACCTGACCAACTTAAGAACCATAAGCCTTATGAAGAACAGTTTTGAGGGTCCATTGCCTGATTTGAGGAAACTTGGCCCACTAAAATCTGTCTACCTGTCGGATAATAAATTATCTGGGGCGATTCCTTCAGATTGGTTCGCAGGAATGTCATCATTGAAGAAAGTACACTTGGCGAATAATCAGTTTACGGGAGAGATTCCAAAGTCGTTGGCAGCTGGGTTGGATAAGTTGGTGGAGCTGAGTATTGAAGGGAACAAATTCGAAGGCAAGATACCAGATTTTAGGCAGCCCGGGTTAACAAATTTTAACGTCTCCAACAACAAATTGGAGGGTGAGATCCCGGAAGGCCTAAGAAAATTTGGTGCAAGCTCATTTGCAGGGAATGAAGGCCTATGTGGAGGGCCAACTAATATAGCATGCAGTACCCACTCGAAGAAGCCCGCACCCTGGATTATCGTGCTGATTGTGATCGCTGTCATCGTAGCAATCCTCGCCATAGTTGCTTTACTGGTCTTCCTTCTTGGACGCCGCAAAAATACATCTGAATCATCGGTAGAGGCTCCACCACCATCAACAGCAACTGTCCAAAACAGATCAGTTCAAAAAGAACTAGAGCACCAAGCAAGTGCTCCGGGATCACCCGAAAACTCGAGCAGAGGGAGTGGAAAGAAGGGTGAACAGAGCTTGAAGCTTTCGTTTGTGAGAGATGACAGGGAGAGGTTTGATATGCAAGACTTGCTGAAAGCCTCGGCGGAGGTGTTAGGCAGCGGTTGTTTTGGGTCATCTTACAAGGCTGCTTTGCTGACCGGACCTGTGATGGTTGTGAAGAGGTATAAGCAGATGAATAATGTGGGAAGAGAGGAGTTCCTAGAGCACATGAGAAGGATTGGGAGATTGGGACATCCTAATTTGCTTCCACTTGTGGCTTATTATTATAAGAAGGAAGAGAAGCTCTTGATTGCTGACCATGTTCAAAACGGCAGCTTGGCTCGTCATCTTCATGGAAACCAAACCCTAGGCCAACCAAGCCTTGACTGGCCAACCCGTTTGAAGATTGTCAAAGGAGTAGCACATGGCTTACGCTATCTCTACGCTGAGCTTCCCAGCCTAGTAGCACCCCATGGCCACCTCAAATCATCAAACGTTCTTCTCAACGAGTCCTACGAGCCCCTTGTCACCGACTACGGCCTAATCCCTGTGGTAAACCAAGAACATGCTCACACCATCATGGTGGCCTACAAGTCCCCAGAGTACATGCAAAGCAAGCGCATTACAAAGAAGACCGATGTTTGGAGCTTCGGAGTGCTGATCTTGGAGATCTTAACGGGGAAGTTGCCCACCAACTTTCTGCAGCAAGGTAAAGGAAGCGAGGAGGATTTGGCAAGTTGGGTGAACTCGGTGCCTCGGGGGGAGTGGTTCAATCAGGTGTTTGATACAGGAATGGGGGCAGGCAAGAGTAGTGAGGGAGAGATGCTGAAGCTCTTGAAAATTGGGTTGGAATGTTGTGAAGGGGAAGTGGAGAAGAGATTGGATCTGAAAGAGGTTGTGGAGAGGATTGAAGAGTTGAAAGAGAAGGACAACCGCGGCGATTCAGATATTTTCTCTACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010769 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016324 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0036211 GO:0040007 GO:0040008 GO:0042995 GO:0043170 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044425 GO:0044459 GO:0044464 GO:0044706 GO:0045177 GO:0045595 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0071944 GO:0080092 GO:0090406 GO:0098590 GO:0120025 GO:0140096 GO:1901564 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

658

Amino Acids

72.52

Weight (kDa)

8.98

Isoelectric Point (pI)

34.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	55 - 95	1.4e-06	Leucine rich repeat N-terminal domain
LRR_8	PF13855	148 - 208	3.9e-08	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	373 - 639	4.5e-30	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	373 - 637	3.7e-27	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009804)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G07040 AT5G35390
fragaria_vesca	FvH4_4g06060 FvH4_5g22250
malus_domestica	MD13G1232600.v1.1 MD16G1238200.v1.1
prunus_persica	Prupe.1G064100_v2.0.a1
pyrus_communis	pycom13g20500 pycom13g20530
rosa_chinensis	RchiOBHm_Chr4g0397621
rosa_laevigata	RLG00000009423
rosa_multiflora	Rmu_co8327933.1_g000001 Rmu_sc0002729.1_g000002 Rmu_sc0005653.1_g000001
rosa_roxburghii	Rroxscaffold_5G00342430
rosa_rugosa	Rorug03G0342300
rosa_samantha	Rh4AG079400 Rh4BG077300 Rh4CG085000 Rh4DG072400
rosa_wichuraiana	Rw4G006500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1299
Acc16I	TGCGCA	1 cut(s)	76
AccB1I	GGYRCC	1 cut(s)	1762
AccI	GTMKAC	1 cut(s)	453
AccII	CGCG	1 cut(s)	1947
AciI	CCGC	7 cut(s)	152, 804, 892, 1115, 1131, 1945, 1947
AclI	AACGTT	1 cut(s)	1506
AclWI	GGATC	3 cut(s)	694, 1006, 1902
AcoI	YGGCCR	2 cut(s)	1400, 1486
AcsI	RAATTY	4 cut(s)	171, 620, 664, 719
AcuI	CTGAAG	2 cut(s)	474, 1859
AcyI	GRCGYC	1 cut(s)	889
AfaI	GTAC	4 cut(s)	10, 528, 787, 1616
AfiI	CCNNNNNNNGG	2 cut(s)	810, 1653
AflII	CTTAAG	1 cut(s)	373
AflIII	ACRYGT	1 cut(s)	130
AjnI	CCWGG	1 cut(s)	809
AjuI	GAANNNNNNNTTGG	6 cut(s)	205, 237, 515, 547, 1841, 1873
Alw21I	GWGCWC	4 cut(s)	106, 981, 994, 1239
Alw26I	GTCTC	1 cut(s)	679
AlwI	GGATC	3 cut(s)	694, 1006, 1902
AlwNI	CAGNNNCTG	2 cut(s)	243, 944
Ama87I	CYCGRG	3 cut(s)	653, 1012, 1767
AoxI	GGCC	9 cut(s)	436, 709, 751, 764, 1381, 1400, 1486, 1549, 1596
ApeKI	GCWGC	7 cut(s)	310, 575, 649, 1128, 1157, 1344, 1714
ApoI	RAATTY	4 cut(s)	171, 620, 664, 719
Asp700I	GAANNNNTTC	1 cut(s)	620
AspA2I	CCTAGG	1 cut(s)	1378
AspLEI	GCGC	2 cut(s)	77, 1632
AspS9I	GGNCC	5 cut(s)	361, 410, 437, 764, 1172
AsuC2I	CCSGG	4 cut(s)	654, 655, 704, 996
AsuHPI	GGTGA	5 cut(s)	707, 993, 1049, 1531, 1765
AsuII	TTCGAA	1 cut(s)	624
AvaI	CYCGRG	3 cut(s)	653, 1012, 1767
AvaII	GGWCC	3 cut(s)	361, 410, 1172
AvrII	CCTAGG	1 cut(s)	1378
BalI	TGGCCA	2 cut(s)	1402, 1488
BanI	GGYRCC	1 cut(s)	1762
BanII	GRGCYC	3 cut(s)	307, 330, 1533
BarI	GAAGNNNNNNTAC	2 cut(s)	618, 650
BauI	CACGAG	1 cut(s)	99
BbsI	GAAGAC	2 cut(s)	868, 1649
Bbv12I	GWGCWC	4 cut(s)	106, 981, 994, 1239
BbvI	GCAGC	7 cut(s)	322, 587, 661, 1140, 1144, 1356, 1726
BccI	CCATC	6 cut(s)	238, 262, 278, 940, 1174, 1595
BceAI	ACGGC	2 cut(s)	1357, 1564
BcgI	CGANNNNNNTGC	2 cut(s)	66, 100
BciT130I	CCWGG	1 cut(s)	811
BcnI	CCSGG	4 cut(s)	654, 655, 704, 996
BcoDI	GTCTC	1 cut(s)	679
BfaI	CTAG	4 cut(s)	974, 1232, 1379, 1472
BfmI	CTRYAG	2 cut(s)	308, 1712
BfrI	CTTAAG	1 cut(s)	373
BglII	AGATCT	1 cut(s)	1680
BisI	GCNGC	9 cut(s)	311, 576, 650, 892, 1129, 1158, 1345, 1715, 1948
BlnI	CCTAGG	1 cut(s)	1378
BlpI	GCTNAGC	1 cut(s)	1458
BlsI	GCNGC	9 cut(s)	312, 577, 651, 893, 1130, 1159, 1346, 1716, 1949
Bme1390I	CCNGG	5 cut(s)	654, 655, 704, 811, 996
Bme18I	GGWCC	3 cut(s)	361, 410, 1172
BmeT110I	CYCGRG	3 cut(s)	653, 1012, 1767
BmgT120I	GGNCC	5 cut(s)	361, 410, 437, 764, 1172
BmiI	GGNNCC	4 cut(s)	193, 411, 924, 1764
BmrFI	CCNGG	5 cut(s)	654, 655, 704, 811, 996
BmsI	GCATC	3 cut(s)	57, 86, 1824
BpiI	GAAGAC	2 cut(s)	868, 1649
Bpu1102I	GCTNAGC	1 cut(s)	1458
Bpu14I	TTCGAA	1 cut(s)	624
BpuEI	CTTGAG	4 cut(s)	164, 314, 335, 341
BpuMI	CCSGG	4 cut(s)	654, 655, 704, 996
BsaAI	YACGTR	2 cut(s)	8, 1971
BsaBI	GATNNNNATC	1 cut(s)	830
BsaHI	GRCGYC	1 cut(s)	889
BsaJI	CCNNGG	7 cut(s)	653, 809, 1110, 1378, 1483, 1766, 1945
BsaWI	WCCGGW	1 cut(s)	1169
Bsc4I	CCNNNNNNNGG	2 cut(s)	810, 1653
Bse1I	ACTGG	4 cut(s)	237, 269, 876, 1403
Bse3DI	GCAATG	1 cut(s)	413
Bse8I	GATNNNNATC	1 cut(s)	830
BseBI	CCWGG	1 cut(s)	811
BseDI	CCNNGG	7 cut(s)	653, 809, 1110, 1378, 1483, 1766, 1945
BseGI	GGATG	2 cut(s)	273, 1264
BseJI	GATNNNNATC	1 cut(s)	830
BseLI	CCNNNNNNNGG	2 cut(s)	810, 1653
BseMI	GCAATG	1 cut(s)	413
BseMII	CTCAG	2 cut(s)	593, 1449
BseNI	ACTGG	4 cut(s)	237, 269, 876, 1403
BseRI	GAGGAG	3 cut(s)	167, 1238, 1748
BseXI	GCAGC	7 cut(s)	322, 587, 661, 1140, 1144, 1356, 1726
BseYI	CCCAGC	2 cut(s)	578, 1466
BsgI	GTGCAG	1 cut(s)	229
Bsh1236I	CGCG	1 cut(s)	1947
BshFI	GGCC	9 cut(s)	438, 711, 753, 766, 1383, 1402, 1488, 1551, 1598
BshNI	GGYRCC	1 cut(s)	1762
BsiHKAI	GWGCWC	4 cut(s)	106, 981, 994, 1239
BsiHKCI	CYCGRG	3 cut(s)	653, 1012, 1767
BsiSI	CCGG	4 cut(s)	654, 704, 995, 1170
BslFI	GGGAC	3 cut(s)	225, 1275, 1591
BslI	CCNNNNNNNGG	2 cut(s)	810, 1653
BsmAI	GTCTC	1 cut(s)	679
BsmBI	CGTCTC	1 cut(s)	679
BsmFI	GGGAC	3 cut(s)	225, 1275, 1591
BsnI	GGCC	9 cut(s)	438, 711, 753, 766, 1383, 1402, 1488, 1551, 1598
BsoBI	CYCGRG	3 cut(s)	653, 1012, 1767
Bsp119I	TTCGAA	1 cut(s)	624
Bsp1286I	GDGCHC	7 cut(s)	106, 307, 330, 981, 994, 1239, 1533
Bsp143I	GATC	7 cut(s)	699, 831, 956, 998, 1671, 1680, 1894
Bsp1720I	GCTNAGC	1 cut(s)	1458
Bsp19I	CCATGG	1 cut(s)	1483
BspACI	CCGC	7 cut(s)	152, 804, 892, 1115, 1131, 1945, 1947
BspANI	GGCC	9 cut(s)	438, 711, 753, 766, 1383, 1402, 1488, 1551, 1598
BspCNI	CTCAG	2 cut(s)	594, 1450
BspFNI	CGCG	1 cut(s)	1947
BspLI	GGNNCC	4 cut(s)	193, 411, 924, 1764
BspMAI	CTGCAG	2 cut(s)	312, 1716
BspPI	GGATC	3 cut(s)	694, 1006, 1902
BspT104I	TTCGAA	1 cut(s)	624
BspT107I	GGYRCC	1 cut(s)	1762
BspTI	CTTAAG	1 cut(s)	373
BsrDI	GCAATG	1 cut(s)	413
BsrI	ACTGG	4 cut(s)	237, 269, 876, 1403
BssECI	CCNNGG	7 cut(s)	653, 809, 1110, 1378, 1483, 1766, 1945
BssMI	GATC	7 cut(s)	699, 831, 956, 998, 1671, 1680, 1894
BssNI	GRCGYC	1 cut(s)	889
BssSI	CACGAG	1 cut(s)	99
BssT1I	CCWWGG	2 cut(s)	1378, 1483
Bst2BI	CACGAG	1 cut(s)	99
Bst2UI	CCWGG	1 cut(s)	811
Bst4CI	ACNGT	2 cut(s)	401, 946
Bst6I	CTCTTC	6 cut(s)	151, 1184, 1213, 1302, 1880, 1917
BstACI	GRCGYC	1 cut(s)	889
BstAFI	CTTAAG	1 cut(s)	373
BstAPI	GCANNNNNTGC	1 cut(s)	1099
BstBAI	YACGTR	2 cut(s)	8, 1971
BstBI	TTCGAA	1 cut(s)	624
BstC8I	GCNNGC	5 cut(s)	730, 781, 804, 1628, 1813
BstDEI	CTNAG	3 cut(s)	602, 713, 1458
BstDSI	CCRYGG	2 cut(s)	1483, 1945
BstF5I	GGATG	2 cut(s)	273, 1264
BstFNI	CGCG	1 cut(s)	1947
BstHHI	GCGC	2 cut(s)	77, 1632
BstKTI	GATC	7 cut(s)	702, 834, 959, 1001, 1674, 1683, 1897
BstMAI	GTCTC	1 cut(s)	679
BstMBI	GATC	7 cut(s)	699, 831, 956, 998, 1671, 1680, 1894
BstMWI	GCNNNNNNNGC	6 cut(s)	149, 854, 863, 1099, 1389, 1627
BstNI	CCWGG	1 cut(s)	811
BstNSI	RCATGY	4 cut(s)	134, 783, 1580, 1621
BstSCI	CCNGG	5 cut(s)	652, 653, 702, 809, 994
BstSFI	CTRYAG	2 cut(s)	308, 1712
BstSNI	TACGTA	1 cut(s)	8
BstUI	CGCG	1 cut(s)	1947
BstV1I	GCAGC	7 cut(s)	322, 587, 661, 1140, 1144, 1356, 1726
BstV2I	GAAGAC	2 cut(s)	868, 1649
BstX2I	RGATCY	3 cut(s)	699, 1680, 1894
BstXI	CCANNNNNNTGG	2 cut(s)	25, 1594
BstYI	RGATCY	3 cut(s)	699, 1680, 1894
BsuRI	GGCC	9 cut(s)	438, 711, 753, 766, 1383, 1402, 1488, 1551, 1598
BtgI	CCRYGG	2 cut(s)	1483, 1945
BtgZI	GCGATG	1 cut(s)	297
BtsCI	GGATG	2 cut(s)	273, 1264
BtsI	GCAGTG	1 cut(s)	77
BtsIMutI	CAGTG	1 cut(s)	77
Cac8I	GCNNGC	5 cut(s)	730, 781, 804, 1628, 1813
CaiI	CAGNNNCTG	2 cut(s)	243, 944
CfoI	GCGC	2 cut(s)	77, 1632
Cfr13I	GGNCC	5 cut(s)	361, 410, 437, 764, 1172
Cfr42I	CCGCGG	1 cut(s)	1948
Cfr9I	CCCGGG	1 cut(s)	653
CseI	GACGC	1 cut(s)	897
Csp6I	GTAC	4 cut(s)	9, 527, 786, 1615
CviQI	GTAC	4 cut(s)	9, 527, 786, 1615
DdeI	CTNAG	3 cut(s)	602, 713, 1458
DpnI	GATC	7 cut(s)	701, 833, 958, 1000, 1673, 1682, 1896
DpnII	GATC	7 cut(s)	699, 831, 956, 998, 1671, 1680, 1894
EaeI	YGGCCR	2 cut(s)	1400, 1486
Eam1104I	CTCTTC	6 cut(s)	151, 1184, 1213, 1302, 1880, 1917
EarI	CTCTTC	6 cut(s)	151, 1184, 1213, 1302, 1880, 1917
EciI	GGCGGA	1 cut(s)	1130
Eco105I	TACGTA	1 cut(s)	8
Eco130I	CCWWGG	2 cut(s)	1378, 1483
Eco147I	AGGCCT	2 cut(s)	711, 753
Eco24I	GRGCYC	3 cut(s)	307, 330, 1533
Eco47I	GGWCC	3 cut(s)	361, 410, 1172
Eco57I	CTGAAG	2 cut(s)	474, 1859
Eco88I	CYCGRG	3 cut(s)	653, 1012, 1767
EcoO109I	RGGNCCY	1 cut(s)	361
EcoRII	CCWGG	1 cut(s)	809
EcoT14I	CCWWGG	2 cut(s)	1378, 1483
EcoT38I	GRGCYC	3 cut(s)	307, 330, 1533
ErhI	CCWWGG	2 cut(s)	1378, 1483
Esp3I	CGTCTC	1 cut(s)	679
FalI	AAGNNNNNCTT	2 cut(s)	179, 211
FaqI	GGGAC	3 cut(s)	225, 1275, 1591
FauI	CCCGC	1 cut(s)	811
FblI	GTMKAC	1 cut(s)	453
Fnu4HI	GCNGC	9 cut(s)	311, 576, 650, 892, 1129, 1158, 1345, 1715, 1948
FokI	GGATG	2 cut(s)	280, 1251
FriOI	GRGCYC	3 cut(s)	307, 330, 1533
Fsp4HI	GCNGC	9 cut(s)	311, 576, 650, 892, 1129, 1158, 1345, 1715, 1948
FspAI	RTGCGCAY	1 cut(s)	76
FspBI	CTAG	4 cut(s)	974, 1232, 1379, 1472
FspI	TGCGCA	1 cut(s)	76
GlaI	GCGC	2 cut(s)	76, 1631
GluI	GCNGC	9 cut(s)	311, 576, 650, 892, 1129, 1158, 1345, 1715, 1948
GsaI	CCCAGC	2 cut(s)	582, 1470
HaeIII	GGCC	9 cut(s)	438, 711, 753, 766, 1383, 1402, 1488, 1551, 1598
HapII	CCGG	4 cut(s)	654, 704, 995, 1170
HgaI	GACGC	1 cut(s)	897
HhaI	GCGC	2 cut(s)	77, 1632
Hin1I	GRCGYC	1 cut(s)	889
Hin6I	GCGC	2 cut(s)	75, 1630
HinP1I	GCGC	2 cut(s)	75, 1630
HincII	GTYRAC	1 cut(s)	660
HindII	GTYRAC	1 cut(s)	660
HindIII	AAGCTT	1 cut(s)	1052
HinfI	GANTC	6 cut(s)	339, 484, 559, 908, 1520, 1952
HpaI	GTTAAC	1 cut(s)	660
HpaII	CCGG	4 cut(s)	654, 704, 995, 1170
HphI	GGTGA	5 cut(s)	707, 993, 1049, 1531, 1765
Hpy166II	GTNNAC	8 cut(s)	349, 454, 529, 549, 660, 1040, 1567, 1756
Hpy188I	TCNGA	7 cut(s)	163, 463, 493, 907, 1664, 1899, 1957
Hpy188III	TCNNGA	2 cut(s)	1318, 1846
Hpy8I	GTNNAC	8 cut(s)	349, 454, 529, 549, 660, 1040, 1567, 1756
Hpy99I	CGWCG	2 cut(s)	212, 262
HpyCH4III	ACNGT	2 cut(s)	401, 946
HpyCH4IV	ACGT	6 cut(s)	7, 34, 257, 672, 1506, 1970
HpyCH4V	TGCA	9 cut(s)	70, 246, 310, 728, 740, 783, 1093, 1621, 1714
HpyF10VI	GCNNNNNNNGC	6 cut(s)	149, 854, 863, 1099, 1389, 1627
HpyF3I	CTNAG	3 cut(s)	602, 713, 1458
HpySE526I	ACGT	6 cut(s)	7, 34, 257, 672, 1506, 1970
Hsp92I	GRCGYC	1 cut(s)	889
HspAI	GCGC	2 cut(s)	75, 1630
KspAI	GTTAAC	1 cut(s)	660
KspI	CCGCGG	1 cut(s)	1948
Kzo9I	GATC	7 cut(s)	699, 831, 956, 998, 1671, 1680, 1894
LmnI	GCTCC	5 cut(s)	28, 598, 928, 997, 1656
Lsp1109I	GCAGC	7 cut(s)	322, 587, 661, 1140, 1144, 1356, 1726
LweI	GCATC	3 cut(s)	57, 86, 1824
MaeI	CTAG	4 cut(s)	974, 1232, 1379, 1472
MaeII	ACGT	6 cut(s)	7, 34, 257, 672, 1506, 1970
MaeIII	GTNAC	3 cut(s)	35, 126, 1537
MalI	GATC	7 cut(s)	701, 833, 958, 1000, 1673, 1682, 1896
MboI	GATC	7 cut(s)	699, 831, 956, 998, 1671, 1680, 1894
MfeI	CAATTG	1 cut(s)	220
MflI	RGATCY	3 cut(s)	699, 1680, 1894
MhlI	GDGCHC	7 cut(s)	106, 307, 330, 981, 994, 1239, 1533
MlsI	TGGCCA	2 cut(s)	1402, 1488
MluNI	TGGCCA	2 cut(s)	1402, 1488
MlyI	GAGTC	1 cut(s)	1529
MmeI	TCCRAC	4 cut(s)	441, 564, 702, 1839
Mox20I	TGGCCA	2 cut(s)	1402, 1488
MroXI	GAANNNNTTC	1 cut(s)	620
MscI	TGGCCA	2 cut(s)	1402, 1488
MseI	TTAA	6 cut(s)	89, 186, 374, 659, 669, 1685
MslI	CAYNNNNRTG	5 cut(s)	23, 135, 1362, 1589, 1592
Msp20I	TGGCCA	2 cut(s)	1402, 1488
MspA1I	CMGCKG	3 cut(s)	578, 1131, 1947
MspCI	CTTAAG	1 cut(s)	373
MspI	CCGG	4 cut(s)	654, 704, 995, 1170
MspR9I	CCNGG	5 cut(s)	654, 655, 704, 811, 996
MunI	CAATTG	1 cut(s)	220
MvaI	CCWGG	1 cut(s)	811
MvnI	CGCG	1 cut(s)	1947
MwoI	GCNNNNNNNGC	6 cut(s)	149, 854, 863, 1099, 1389, 1627
NciI	CCSGG	4 cut(s)	654, 655, 704, 996
NcoI	CCATGG	1 cut(s)	1483
NdeII	GATC	7 cut(s)	699, 831, 956, 998, 1671, 1680, 1894
NlaIV	GGNNCC	4 cut(s)	193, 411, 924, 1764
NmeAIII	GCCGAG	1 cut(s)	1091
NmuCI	GTSAC	1 cut(s)	1537
NsbI	TGCGCA	1 cut(s)	76
NspI	RCATGY	4 cut(s)	134, 783, 1580, 1621
NspV	TTCGAA	1 cut(s)	624
PaeI	GCATGC	1 cut(s)	783
PaeR7I	CTCGAG	1 cut(s)	1012
PceI	AGGCCT	2 cut(s)	711, 753
PciI	ACATGT	1 cut(s)	130
PcsI	WCGNNNNNNNCGW	2 cut(s)	840, 1524
PdmI	GAANNNNTTC	1 cut(s)	620
PfeI	GAWTC	5 cut(s)	339, 484, 559, 908, 1952
PfoI	TCCNGGA	2 cut(s)	702, 994
PkrI	GCNGC	9 cut(s)	312, 577, 651, 893, 1130, 1159, 1346, 1716, 1949
PleI	GAGTC	1 cut(s)	1528
PpsI	GAGTC	1 cut(s)	1528
Ppu21I	YACGTR	2 cut(s)	8, 1971
PpuMI	RGGWCCY	1 cut(s)	361
PscI	ACATGT	1 cut(s)	130
PsiI	TTATAA	1 cut(s)	1299
Psp1406I	AACGTT	1 cut(s)	1506
Psp5II	RGGWCCY	1 cut(s)	361
Psp6I	CCWGG	1 cut(s)	809
PspFI	CCCAGC	2 cut(s)	578, 1466
PspGI	CCWGG	1 cut(s)	809
PspN4I	GGNNCC	4 cut(s)	193, 411, 924, 1764
PspPI	GGNCC	5 cut(s)	361, 410, 437, 764, 1172
PspPPI	RGGWCCY	1 cut(s)	361
PspXI	VCTCGAGB	1 cut(s)	1012
PstI	CTGCAG	2 cut(s)	312, 1716
PstNI	CAGNNNCTG	2 cut(s)	243, 944
PsuI	RGATCY	3 cut(s)	699, 1680, 1894
PvuII	CAGCTG	1 cut(s)	578
RsaI	GTAC	4 cut(s)	10, 528, 787, 1616
RsaNI	GTAC	4 cut(s)	9, 527, 786, 1615
RseI	CAYNNNNRTG	5 cut(s)	23, 135, 1362, 1589, 1592
SacII	CCGCGG	1 cut(s)	1948
SaqAI	TTAA	6 cut(s)	89, 186, 374, 659, 669, 1685
SatI	GCNGC	9 cut(s)	311, 576, 650, 892, 1129, 1158, 1345, 1715, 1948
Sau3AI	GATC	7 cut(s)	699, 831, 956, 998, 1671, 1680, 1894
Sau96I	GGNCC	5 cut(s)	361, 410, 437, 764, 1172
SchI	GAGTC	1 cut(s)	1529
ScrFI	CCNGG	5 cut(s)	654, 655, 704, 811, 996
SduI	GDGCHC	7 cut(s)	106, 307, 330, 981, 994, 1239, 1533
SfaNI	GCATC	3 cut(s)	57, 86, 1824
SfcI	CTRYAG	2 cut(s)	308, 1712
Sfr274I	CTCGAG	1 cut(s)	1012
Sfr303I	CCGCGG	1 cut(s)	1948
SfuI	TTCGAA	1 cut(s)	624
SgrBI	CCGCGG	1 cut(s)	1948
SinI	GGWCC	3 cut(s)	361, 410, 1172
SlaI	CTCGAG	1 cut(s)	1012
SmaI	CCCGGG	1 cut(s)	655
SmiMI	CAYNNNNRTG	5 cut(s)	23, 135, 1362, 1589, 1592
SmlI	CTYRAG	6 cut(s)	179, 314, 329, 356, 373, 1012
SmoI	CTYRAG	6 cut(s)	179, 314, 329, 356, 373, 1012
SnaBI	TACGTA	1 cut(s)	8
SphI	GCATGC	1 cut(s)	783
SseBI	AGGCCT	2 cut(s)	711, 753
SsiI	CCGC	7 cut(s)	152, 804, 892, 1115, 1131, 1945, 1947
SspMI	CTAG	4 cut(s)	974, 1232, 1379, 1472
StuI	AGGCCT	2 cut(s)	711, 753
StyD4I	CCNGG	5 cut(s)	652, 653, 702, 809, 994
StyI	CCWWGG	2 cut(s)	1378, 1483
TaaI	ACNGT	2 cut(s)	401, 946
TaiI	ACGT	6 cut(s)	10, 37, 260, 675, 1509, 1973
TaqI	TCGA	5 cut(s)	233, 260, 624, 794, 1013
TaqII	GACCGA	1 cut(s)	1661
TatI	WGTACW	2 cut(s)	526, 1614
TauI	GCSGC	2 cut(s)	894, 1950
TfiI	GAWTC	5 cut(s)	339, 484, 559, 908, 1952
Tru1I	TTAA	6 cut(s)	89, 186, 374, 659, 669, 1685
Tru9I	TTAA	6 cut(s)	89, 186, 374, 659, 669, 1685
TscAI	CASTG	1 cut(s)	77
TseFI	GTSAC	1 cut(s)	1537
TseI	GCWGC	7 cut(s)	310, 575, 649, 1128, 1157, 1344, 1714
Tsp45I	GTSAC	1 cut(s)	1537
TspDTI	ATGAA	6 cut(s)	62, 68, 407, 762, 1220, 1352
TspGWI	ACGGA	1 cut(s)	225
TspMI	CCCGGG	1 cut(s)	653
TspRI	CASTG	1 cut(s)	77
Vha464I	CTTAAG	1 cut(s)	373
VpaK11BI	GGWCC	3 cut(s)	361, 410, 1172
XapI	RAATTY	4 cut(s)	171, 620, 664, 719
XceI	RCATGY	4 cut(s)	134, 783, 1580, 1621
XcmI	CCANNNNNNNNNTGG	2 cut(s)	685, 1395
XhoI	CTCGAG	1 cut(s)	1012
XmaI	CCCGGG	1 cut(s)	653
XmaJI	CCTAGG	1 cut(s)	1378
XmiI	GTMKAC	1 cut(s)	453
XmnI	GAANNNNTTC	1 cut(s)	620
XspI	CTAG	4 cut(s)	974, 1232, 1379, 1472

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.