Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007699

Physical Location & Seq

Genomic Coordinates Forward (+)

35109855 .. 35121185

Gene Structure 11331 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1270600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 828 bp

ATGAGCTTTCGGGATCTGGAAGCGGGTCGGGGCACCAATTATTCCCGGCGAGGAGACCTCATCAATGGCAAGCGGGACCCCACCCAAGCTGTGGCCTCCGGCATTTTCCAGATCAACACCGCCGTATCCACCTTCCAGAGGCTCGTCAACACCATCGGCACCCCCAAAGACACGCCGGATCTCCGCGAAAAGCTGCACAAGACAAGACTACATATCGGGCAATTGGTGAAGGATACTTCAGAAAAACTTAAACAAGTCAGTGAAAGAGATCATCATACCGAAGTTAATGCAAGCAAGAAGATTACAGATGCTAAACTTGCAAAAGATTTTCAAGCAGTGCTTAGAGAATTTCAGAAGGCTCAACGACTTGCAGCTGAGAGGGAAACAACATATGCTCCTTTTGTTCCTCAAGCAGTTCTTCCGACAAGTTACACCGCTAGCGAGATAGATGTAAACTCAGACAAAAGTCTGGAACAGCGTGCTCTCCTTGTGGAATCTAGAAGACAAGAGGTCTTGCTACTGGACAATGAGATTGCTTTCAATGAGGCTATCATCGAGGAAAGAGAACAGGGAATACAAGAAATCCAGCAGCAAATTGGTGAAGTGAATGAGATTTTTAAAGATCTTGCTATACTGGTTCATGAACAAGGAACAATGATTGATGACATTGGCTCCAATATTGAGAGTGCTCATGCTGCTACTGGGCAGGCAAAGTCCCATCTAGTGAAGGCGTCAAAGACCCAAAGATCGAATTCATCTCTGACTTGCTTGCTCTTGGTAATATTTGGAATTGTGCTTCTCATTGTGATAGTAGTACTTGCAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0000325 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006886 GO:0006906 GO:0006996 GO:0008104 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009657 GO:0009660 GO:0009705 GO:0009959 GO:0009987 GO:0010118 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0022406 GO:0031090 GO:0031201 GO:0031224 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048278 GO:0048284 GO:0050896 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0061024 GO:0061025 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0090174 GO:0098588 GO:0098796 GO:0098805 GO:0140056

KEGG Pathways

Metabolic & Signaling

ko04130 ko04145 map04130 map04145

Pfam Domains

Protein Families

SNARE Syntaxin Syntaxin_2

Protein Analysis

276

Amino Acids

30.57

Weight (kDa)

6.2

Isoelectric Point (pI)

38.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syntaxin_2	PF14523	28 - 163	2.9e-37	Syntaxin-like protein
SNARE	PF05739	218 - 270	7.3e-16	SNARE domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007699)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17730 AT4G17730 AT5G46860
fragaria_vesca	FvH4_5g22860 FvH4_5g22860
malus_domestica	MD06G1038300.v1.1 MD16G1270600.v1.1
prunus_persica	Prupe.5G030000_v2.0.a1 Prupe.5G030000_v2.0.a1
pyrus_communis	pycom06g03200 pycom16g23960
rosa_chinensis	RchiOBHm_Chr7g0211551
rosa_laevigata	RLG00000002929 RLG00000002999
rosa_multiflora	Rmu_co8509339.1_g000001 Rmu_sc0011240.1_g000011 Rmu_sc0012097.1_g000010
rosa_roxburghii	Rroxscaffold_3G00247770
rosa_rugosa	Rorug07G0125900
rosa_samantha	Rh7AG258100 Rh7BG252300 Rh7CG275200 Rh7DG265700
rosa_wichuraiana	Rw7G022080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	32, 158
AccB7I	CCANNNNNTGG	1 cut(s)	91
AccII	CGCG	1 cut(s)	186
AciI	CCGC	5 cut(s)	23, 73, 120, 184, 435
AclWI	GGATC	2 cut(s)	21, 186
AcsI	RAATTY	2 cut(s)	347, 751
AcuI	CTGAAG	1 cut(s)	222
AcyI	GRCGYC	1 cut(s)	731
AfaI	GTAC	1 cut(s)	816
AfiI	CCNNNNNNNGG	2 cut(s)	91, 138
AgsI	TTSAA	2 cut(s)	332, 541
AhdI	GACNNNNNGTC	1 cut(s)	509
AluBI	AGCT	5 cut(s)	6, 89, 193, 374, 824
AluI	AGCT	5 cut(s)	6, 89, 193, 374, 824
Alw21I	GWGCWC	2 cut(s)	484, 691
Alw26I	GTCTC	1 cut(s)	48
AlwI	GGATC	2 cut(s)	21, 186
AoxI	GGCC	1 cut(s)	93
ApeKI	GCWGC	5 cut(s)	193, 371, 589, 695, 821
ApoI	RAATTY	2 cut(s)	347, 751
AspS9I	GGNCC	1 cut(s)	76
AsuC2I	CCSGG	1 cut(s)	46
AsuHPI	GGTGA	2 cut(s)	238, 611
AsuNHI	GCTAGC	1 cut(s)	437
AvaII	GGWCC	1 cut(s)	76
BaeGI	GKGCMC	1 cut(s)	35
BaeI	ACNNNNGTAYC	2 cut(s)	108, 141
BanI	GGYRCC	2 cut(s)	32, 158
BbsI	GAAGAC	1 cut(s)	508
Bbv12I	GWGCWC	2 cut(s)	484, 691
BbvI	GCAGC	4 cut(s)	180, 383, 601, 682
BccI	CCATC	2 cut(s)	161, 726
BceAI	ACGGC	1 cut(s)	107
BcgI	CGANNNNNNTGC	2 cut(s)	269, 303
BciVI	GTATCC	2 cut(s)	136, 226
BcnI	CCSGG	1 cut(s)	46
BcoDI	GTCTC	1 cut(s)	48
BfaI	CTAG	3 cut(s)	438, 498, 722
BfuI	GTATCC	2 cut(s)	136, 226
BglII	AGATCT	1 cut(s)	622
BisI	GCNGC	5 cut(s)	194, 372, 590, 696, 822
BlsI	GCNGC	5 cut(s)	195, 373, 591, 697, 823
BmcAI	AGTACT	1 cut(s)	816
Bme1390I	CCNGG	1 cut(s)	46
Bme18I	GGWCC	1 cut(s)	76
BmeRI	GACNNNNNGTC	1 cut(s)	509
BmgT120I	GGNCC	1 cut(s)	76
BmiI	GGNNCC	5 cut(s)	34, 77, 78, 160, 673
BmrFI	CCNGG	1 cut(s)	46
BmrI	ACTGGG	1 cut(s)	711
BmsI	GCATC	1 cut(s)	298
BmtI	GCTAGC	1 cut(s)	441
BmuI	ACTGGG	1 cut(s)	711
BoxI	GACNNNNGTC	1 cut(s)	465
BpiI	GAAGAC	1 cut(s)	508
BplI	GAGNNNNNCTC	2 cut(s)	42, 74
BpuEI	CTTGAG	1 cut(s)	393
BpuMI	CCSGG	1 cut(s)	46
BsaHI	GRCGYC	1 cut(s)	731
BsaI	GGTCTC	1 cut(s)	48
BsaXI	ACNNNNNCTCC	4 cut(s)	379, 409, 656, 686
Bsc4I	CCNNNNNNNGG	2 cut(s)	91, 138
Bse1I	ACTGG	3 cut(s)	525, 639, 706
BseLI	CCNNNNNNNGG	2 cut(s)	91, 138
BseMII	CTCAG	2 cut(s)	366, 471
BseNI	ACTGG	3 cut(s)	525, 639, 706
BseRI	GAGGAG	1 cut(s)	66
BseSI	GKGCMC	1 cut(s)	35
BseXI	GCAGC	4 cut(s)	180, 383, 601, 682
BsgI	GTGCAG	1 cut(s)	179
Bsh1236I	CGCG	1 cut(s)	186
BshFI	GGCC	1 cut(s)	95
BshNI	GGYRCC	2 cut(s)	32, 158
BsiHKAI	GWGCWC	2 cut(s)	484, 691
BsiSI	CCGG	3 cut(s)	46, 99, 176
BslFI	GGGAC	2 cut(s)	89, 700
BslI	CCNNNNNNNGG	2 cut(s)	91, 138
BsmAI	GTCTC	1 cut(s)	48
BsmFI	GGGAC	2 cut(s)	89, 700
BsnI	GGCC	1 cut(s)	95
Bso31I	GGTCTC	1 cut(s)	48
Bsp1286I	GDGCHC	3 cut(s)	35, 484, 691
Bsp143I	GATC	6 cut(s)	13, 111, 178, 268, 622, 746
BspACI	CCGC	5 cut(s)	23, 73, 120, 184, 435
BspANI	GGCC	1 cut(s)	95
BspCNI	CTCAG	2 cut(s)	367, 470
BspFNI	CGCG	1 cut(s)	186
BspHI	TCATGA	1 cut(s)	640
BspLI	GGNNCC	5 cut(s)	34, 77, 78, 160, 673
BspOI	GCTAGC	1 cut(s)	441
BspPI	GGATC	2 cut(s)	21, 186
BspT107I	GGYRCC	2 cut(s)	32, 158
BspTNI	GGTCTC	1 cut(s)	48
BsrI	ACTGG	3 cut(s)	525, 639, 706
BssMI	GATC	6 cut(s)	13, 111, 178, 268, 622, 746
BssNI	GRCGYC	1 cut(s)	731
BstACI	GRCGYC	1 cut(s)	731
BstC8I	GCNNGC	6 cut(s)	71, 292, 439, 480, 708, 770
BstDEI	CTNAG	3 cut(s)	341, 375, 457
BstENI	CCTNNNNNAGG	1 cut(s)	136
BstFNI	CGCG	1 cut(s)	186
BstKTI	GATC	6 cut(s)	16, 114, 181, 271, 625, 749
BstMAI	GTCTC	1 cut(s)	48
BstMBI	GATC	6 cut(s)	13, 111, 178, 268, 622, 746
BstMWI	GCNNNNNNNGC	2 cut(s)	317, 695
BstPAI	GACNNNNGTC	1 cut(s)	465
BstSCI	CCNGG	1 cut(s)	44
BstSLI	GKGCMC	1 cut(s)	35
BstUI	CGCG	1 cut(s)	186
BstV1I	GCAGC	4 cut(s)	180, 383, 601, 682
BstV2I	GAAGAC	1 cut(s)	508
BstX2I	RGATCY	3 cut(s)	13, 178, 622
BstYI	RGATCY	3 cut(s)	13, 178, 622
BsuI	GTATCC	2 cut(s)	136, 226
BsuRI	GGCC	1 cut(s)	95
BtsI	GCAGTG	1 cut(s)	342
BtsIMutI	CAGTG	2 cut(s)	265, 342
Cac8I	GCNNGC	6 cut(s)	71, 292, 439, 480, 708, 770
CciI	TCATGA	1 cut(s)	640
Cfr13I	GGNCC	1 cut(s)	76
CseI	GACGC	1 cut(s)	720
Csp6I	GTAC	1 cut(s)	815
CviAII	CATG	2 cut(s)	641, 692
CviQI	GTAC	1 cut(s)	815
DdeI	CTNAG	3 cut(s)	341, 375, 457
DpnI	GATC	6 cut(s)	15, 113, 180, 270, 624, 748
DpnII	GATC	6 cut(s)	13, 111, 178, 268, 622, 746
DraI	TTTAAA	1 cut(s)	619
DriI	GACNNNNNGTC	1 cut(s)	509
Eam1105I	GACNNNNNGTC	1 cut(s)	509
Eco31I	GGTCTC	1 cut(s)	48
Eco47I	GGWCC	1 cut(s)	76
Eco57I	CTGAAG	1 cut(s)	222
EcoNI	CCTNNNNNAGG	1 cut(s)	136
EcoO109I	RGGNCCY	1 cut(s)	76
EcoRI	GAATTC	1 cut(s)	751
FaeI	CATG	2 cut(s)	644, 695
FaiI	YATR	7 cut(s)	213, 276, 391, 393, 632, 642, 693
FalI	AAGNNNNNCTT	4 cut(s)	324, 356, 402, 434
FaqI	GGGAC	2 cut(s)	89, 700
FatI	CATG	2 cut(s)	640, 691
FauI	CCCGC	2 cut(s)	16, 66
FauNDI	CATATG	1 cut(s)	391
Fnu4HI	GCNGC	5 cut(s)	194, 372, 590, 696, 822
Fsp4HI	GCNGC	5 cut(s)	194, 372, 590, 696, 822
FspBI	CTAG	3 cut(s)	438, 498, 722
GluI	GCNGC	5 cut(s)	194, 372, 590, 696, 822
HaeIII	GGCC	1 cut(s)	95
HapII	CCGG	3 cut(s)	46, 99, 176
HgaI	GACGC	1 cut(s)	720
Hin1I	GRCGYC	1 cut(s)	731
Hin1II	CATG	2 cut(s)	644, 695
HincII	GTYRAC	1 cut(s)	148
HindII	GTYRAC	1 cut(s)	148
HinfI	GANTC	1 cut(s)	494
HpaII	CCGG	3 cut(s)	46, 99, 176
HphI	GGTGA	2 cut(s)	238, 611
Hpy166II	GTNNAC	2 cut(s)	148, 454
Hpy188I	TCNGA	5 cut(s)	241, 354, 423, 460, 762
Hpy188III	TCNNGA	7 cut(s)	11, 17, 109, 136, 470, 498, 641
Hpy8I	GTNNAC	2 cut(s)	148, 454
HpyAV	CCTTC	4 cut(s)	142, 223, 349, 721
HpyCH4V	TGCA	5 cut(s)	196, 290, 320, 371, 821
HpyF10VI	GCNNNNNNNGC	2 cut(s)	317, 695
HpyF3I	CTNAG	3 cut(s)	341, 375, 457
Hsp92I	GRCGYC	1 cut(s)	731
Hsp92II	CATG	2 cut(s)	644, 695
KflI	GGGWCCC	1 cut(s)	76
Kzo9I	GATC	6 cut(s)	13, 111, 178, 268, 622, 746
LmnI	GCTCC	2 cut(s)	400, 677
Lsp1109I	GCAGC	4 cut(s)	180, 383, 601, 682
LweI	GCATC	1 cut(s)	298
MaeI	CTAG	3 cut(s)	438, 498, 722
MaeIII	GTNAC	1 cut(s)	428
MalI	GATC	6 cut(s)	15, 113, 180, 270, 624, 748
MboI	GATC	6 cut(s)	13, 111, 178, 268, 622, 746
MboII	GAAGA	3 cut(s)	310, 410, 513
MfeI	CAATTG	1 cut(s)	221
MflI	RGATCY	3 cut(s)	13, 178, 622
MhlI	GDGCHC	3 cut(s)	35, 484, 691
MluCI	AATT	6 cut(s)	37, 221, 347, 594, 751, 789
MmeI	TCCRAC	1 cut(s)	446
MnlI	CCTC	9 cut(s)	44, 68, 106, 132, 372, 417, 502, 538, 550
MseI	TTAA	4 cut(s)	249, 285, 618, 826
MspA1I	CMGCKG	1 cut(s)	374
MspI	CCGG	3 cut(s)	46, 99, 176
MspR9I	CCNGG	1 cut(s)	46
MunI	CAATTG	1 cut(s)	221
MvnI	CGCG	1 cut(s)	186
MwoI	GCNNNNNNNGC	2 cut(s)	317, 695
NciI	CCSGG	1 cut(s)	46
NdeI	CATATG	1 cut(s)	391
NdeII	GATC	6 cut(s)	13, 111, 178, 268, 622, 746
NheI	GCTAGC	1 cut(s)	437
NlaIII	CATG	2 cut(s)	644, 695
NlaIV	GGNNCC	5 cut(s)	34, 77, 78, 160, 673
PagI	TCATGA	1 cut(s)	640
PfeI	GAWTC	1 cut(s)	494
PflMI	CCANNNNNTGG	1 cut(s)	91
PkrI	GCNGC	5 cut(s)	195, 373, 591, 697, 823
PpuMI	RGGWCCY	1 cut(s)	76
PshAI	GACNNNNGTC	1 cut(s)	465
Psp5II	RGGWCCY	1 cut(s)	76
PspN4I	GGNNCC	5 cut(s)	34, 77, 78, 160, 673
PspPI	GGNCC	1 cut(s)	76
PspPPI	RGGWCCY	1 cut(s)	76
PsrI	GAACNNNNNNTAC	2 cut(s)	558, 590
PsuI	RGATCY	3 cut(s)	13, 178, 622
PvuII	CAGCTG	1 cut(s)	374
RsaI	GTAC	1 cut(s)	816
RsaNI	GTAC	1 cut(s)	815
SaqAI	TTAA	4 cut(s)	249, 285, 618, 826
SatI	GCNGC	5 cut(s)	194, 372, 590, 696, 822
Sau3AI	GATC	6 cut(s)	13, 111, 178, 268, 622, 746
Sau96I	GGNCC	1 cut(s)	76
ScaI	AGTACT	1 cut(s)	816
ScrFI	CCNGG	1 cut(s)	46
SduI	GDGCHC	3 cut(s)	35, 484, 691
SetI	ASST	8 cut(s)	8, 60, 91, 134, 195, 376, 513, 826
SfaNI	GCATC	1 cut(s)	298
SinI	GGWCC	1 cut(s)	76
SmlI	CTYRAG	1 cut(s)	408
SmoI	CTYRAG	1 cut(s)	408
Sse9I	AATT	6 cut(s)	37, 221, 347, 594, 751, 789
SsiI	CCGC	5 cut(s)	23, 73, 120, 184, 435
SspI	AATATT	2 cut(s)	679, 783
SspMI	CTAG	3 cut(s)	438, 498, 722
StyD4I	CCNGG	1 cut(s)	44
TaqI	TCGA	2 cut(s)	555, 749
TasI	AATT	6 cut(s)	37, 221, 347, 594, 751, 789
TatI	WGTACW	1 cut(s)	814
TfiI	GAWTC	1 cut(s)	494
Tru1I	TTAA	4 cut(s)	249, 285, 618, 826
Tru9I	TTAA	4 cut(s)	249, 285, 618, 826
TscAI	CASTG	2 cut(s)	265, 342
TseI	GCWGC	5 cut(s)	193, 371, 589, 695, 821
TspDTI	ATGAA	3 cut(s)	629, 657, 744
TspRI	CASTG	2 cut(s)	265, 342
Van91I	CCANNNNNTGG	1 cut(s)	91
VpaK11BI	GGWCC	1 cut(s)	76
XagI	CCTNNNNNAGG	1 cut(s)	136
XapI	RAATTY	2 cut(s)	347, 751
XbaI	TCTAGA	1 cut(s)	497
XcmI	CCANNNNNNNNNTGG	2 cut(s)	88, 593
XspI	CTAG	3 cut(s)	438, 498, 722
ZrmI	AGTACT	1 cut(s)	816

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.