Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008082

Physical Location & Seq

Genomic Coordinates Forward (+)

1804584 .. 1806517

Gene Structure 1934 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1024800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1605 bp

ATGAAAATGAGGGAAGTCCTTGCTAATCTGCTTGCATCATGTAACAGCGCGGAATCGGCGGCGCAATTGCACTCTCAAACCCTCAAAGCCGGCCTTTCCCAGGACAGCTTATTTGCCACAAAACTCAACGCTCTGTACGCAAAATATGAGTGCATCGGTCATGCCCGCAAGGTGTTCGACGAAACGCCCCACAGAATTGTCTATCTCTGGAACGCCATGCTCAGGAGCTACTGTAGAGAGGACCGGTGGGAGGAGACCCTGTGTCTATTTTGTAGCATGATGTCTGAGTTAAGAGGTAATGATGAAAAGCCTGATAATTTCACCATTCCTATTGCTCTGAAGGCGTGTGCGAGGTTAAGGGCTCTAGCGTGTGGAAAAATAATACATGGGTTTGTGAAGAAACATGAGAAGGTTGCATTGGATATGTTTGTAGGTTCTGCATTGATTGAATTGTATTCCAAATACGGAGAAATGGGCGAGGCTGTGAAAGTGGTTGACGAGTTTTTGCACCCGGATGTGTTTTTGTGGACTTCTATGGTTACTGGATATGAGCAAAATGGTGATCCTGAAGAAGCATTGGAGTTTTTCTCCCGAATGGTGATGGTGGGGCGCATTAATCATGACCGAGTGACGCTTGTTAGTGCTGTTTCGACTTGTGCTAAGTTATCAAATTTTAGAATTGGAAGTTGTGTGCATGATGTTTCTATTAGGAACGGGTTCAACTCTGATTTATCTTTAGGTAATGCATTGCTAAATCTCTATGCAAAGACGGGTTCTGTCAAAACTGCCGCTAAGTTGTTCATGAAGATGCCGGAAAAAGATGTGGTTTCTTGGAGCTCCATGATTGCGTGTTACACTCATAATGGGGATGTAATAGAAGCGTTAAATCTTTTCAACGAAATGATTGATATGAGAACCGAACCTAATTCAGTGACTCTGGTTAGTGCATTGCAAGCATGTGCACTGGCAGGTAATTTAGAAGAAGGTGAGAAGATCCATGAAACTGCTACTAGGAAGTGTTTCGAGTTAGATATCAAGGTAGCTACAGCCCTGATAGATATGTACATGAAGTGCTCTGCACCCGAAAAAGCAGGTGATCTCTTCAATAGAGTGCCAGAGAAAGATGTGGTTTCGTGGGCTGCCTTGTTGAGCGGGTATGCTCATAACGGAATGACATACAAGTCAATTGGGGTCTTTCGCGACATGCTATCAGACGAAACTAAGCCTGATGCGTTGCTATGCCATTCGGGTTTGGTTGAAGAAGGCATCGAGATATTCAAGACCATGTTGCCTGAATACCAACTTAAGCCCGAACCTGAGCACTACGGGATAATTGTTGATCTTCTTGGCCGGATAGGAGAGCTGGATAAGGCAATGGAAATCGTTGAGAGAATGCCCAATCCAGCTGCACCGCATGTTTGGGGAGCCTTGCTTGGTGCATGCCGGATTCACAACAACACAAAGCTTGGAGAGGTTGCAGCAAAGAGTCTTTTTCGGTTGGATCCTAATCATGCAGGATATTACATCTTGCTCTCGAATATATATGCCATGGATAACAAATGGGAAAATGTGACAAATCTTAGAACTTTGATAAAGGAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

535

Amino Acids

59.68

Weight (kDa)

5.93

Isoelectric Point (pI)

34.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	67 - 118	4.1e-06	PPR repeat family
PPR	PF01535	175 - 201	1.1e-06	PPR repeat
PPR_2	PF13041	273 - 321	2.8e-10	PPR repeat family
PPR	PF01535	276 - 303	7.1e-08	PPR repeat
E_motif	PF20431	484 - 533	1.2e-10	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008082)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01580
fragaria_vesca	FvH4_6g51580 FvH4_6g51620
malus_domestica	MD07G1177500.v1.1 MD17G1023700.v1.1 MD17G1024400.v1.1 MD17G1024800.v1.1 MD17G1025100.v1.1 MD17G1025800.v1.1 MD17G1026000.v1.1
prunus_persica	Prupe.3G292700_v2.0.a1
pyrus_communis	pycom07g17150 pycom17g02080 pycom17g02110
rosa_laevigata	RLG00000022166
rosa_multiflora	Rmu_sc0000860.1_g000001 Rmu_sc0016793.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079160
rosa_rugosa	Rorug02G0565900
rosa_samantha	Rh2AG646100 Rh2BG656800 Rh2CG621900 Rh2DG670200
rosa_wichuraiana	Rw2G053060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1082
AasI	GACNNNNNNGTC	1 cut(s)	1180
Acc36I	ACCTGC	2 cut(s)	959, 1082
AccBSI	CCGCTC	1 cut(s)	1152
AccII	CGCG	2 cut(s)	50, 1200
AciI	CCGC	6 cut(s)	50, 59, 166, 789, 1152, 1412
AclWI	GGATC	4 cut(s)	557, 988, 1496, 1509
AcoI	YGGCCR	1 cut(s)	1348
AcsI	RAATTY	1 cut(s)	670
AcuI	CTGAAG	2 cut(s)	359, 588
AfaI	GTAC	2 cut(s)	137, 1064
AfiI	CCNNNNNNNGG	3 cut(s)	100, 222, 250
AflII	CTTAAG	1 cut(s)	1304
AgeI	ACCGGT	1 cut(s)	243
AgsI	TTSAA	6 cut(s)	449, 721, 895, 1105, 1259, 1279
AhdI	GACNNNNNGTC	1 cut(s)	261
AjnI	CCWGG	1 cut(s)	99
AjuI	GAANNNNNNNTTGG	2 cut(s)	401, 433
AluBI	AGCT	7 cut(s)	108, 228, 837, 1043, 1363, 1406, 1465
AluI	AGCT	7 cut(s)	108, 228, 837, 1043, 1363, 1406, 1465
Alw21I	GWGCWC	4 cut(s)	839, 964, 1076, 1323
Alw26I	GTCTC	1 cut(s)	248
Alw44I	GTGCAC	1 cut(s)	960
AlwI	GGATC	4 cut(s)	557, 988, 1496, 1509
AoxI	GGCC	2 cut(s)	91, 1348
ApaLI	GTGCAC	1 cut(s)	960
ApeKI	GCWGC	3 cut(s)	1139, 1406, 1478
ApoI	RAATTY	1 cut(s)	670
ArsI	GACNNNNNNTTYG	2 cut(s)	95, 127
AseI	ATTAAT	1 cut(s)	615
AsiGI	ACCGGT	1 cut(s)	243
Asp700I	GAANNNNTTC	2 cut(s)	716, 1019
AspLEI	GCGC	3 cut(s)	50, 64, 612
AspS9I	GGNCC	1 cut(s)	241
AsuC2I	CCSGG	1 cut(s)	512
AsuHPI	GGTGA	5 cut(s)	313, 572, 610, 998, 1106
AvaII	GGWCC	1 cut(s)	241
BaeGI	GKGCMC	1 cut(s)	964
BamHI	GGATCC	1 cut(s)	1501
BanII	GRGCYC	2 cut(s)	364, 839
Bbv12I	GWGCWC	4 cut(s)	839, 964, 1076, 1323
BbvI	GCAGC	3 cut(s)	1126, 1393, 1490
BccI	CCATC	1 cut(s)	595
BcgI	CGANNNNNNTGC	2 cut(s)	157, 191
BciT130I	CCWGG	1 cut(s)	101
BcnI	CCSGG	1 cut(s)	512
BcoDI	GTCTC	1 cut(s)	248
BfaI	CTAG	2 cut(s)	365, 1011
BfmI	CTRYAG	2 cut(s)	232, 1044
BfrI	CTTAAG	1 cut(s)	1304
BfuAI	ACCTGC	2 cut(s)	959, 1082
BisI	GCNGC	5 cut(s)	60, 789, 1140, 1407, 1479
BlsI	GCNGC	5 cut(s)	61, 790, 1141, 1408, 1480
Bme1390I	CCNGG	2 cut(s)	101, 512
Bme18I	GGWCC	1 cut(s)	241
BmeRI	GACNNNNNGTC	1 cut(s)	261
BmgT120I	GGNCC	1 cut(s)	241
BmiI	GGNNCC	2 cut(s)	1426, 1503
BmrFI	CCNGG	2 cut(s)	101, 512
BmsI	GCATC	5 cut(s)	44, 162, 798, 1219, 1275
BplI	GAGNNNNNCTC	2 cut(s)	572, 604
Bpu10I	CCTNAGC	2 cut(s)	221, 1317
BpuMI	CCSGG	1 cut(s)	512
BsaBI	GATNNNNATC	1 cut(s)	1506
BsaI	GGTCTC	1 cut(s)	248
BsaJI	CCNNGG	2 cut(s)	99, 1548
BsaWI	WCCGGW	1 cut(s)	243
BsaXI	ACNNNNNCTCC	4 cut(s)	217, 245, 247, 275
Bsc4I	CCNNNNNNNGG	3 cut(s)	100, 222, 250
Bse118I	RCCGGY	2 cut(s)	89, 243
Bse1I	ACTGG	2 cut(s)	547, 969
Bse3DI	GCAATG	3 cut(s)	746, 947, 1380
Bse8I	GATNNNNATC	1 cut(s)	1506
BseBI	CCWGG	1 cut(s)	101
BseDI	CCNNGG	2 cut(s)	99, 1548
BseGI	GGATG	2 cut(s)	520, 874
BseJI	GATNNNNATC	1 cut(s)	1506
BseLI	CCNNNNNNNGG	3 cut(s)	100, 222, 250
BseMI	GCAATG	3 cut(s)	746, 947, 1380
BseMII	CTCAG	3 cut(s)	235, 276, 1308
BseNI	ACTGG	2 cut(s)	547, 969
BseRI	GAGGAG	1 cut(s)	266
BseSI	GKGCMC	1 cut(s)	964
BseXI	GCAGC	3 cut(s)	1126, 1393, 1490
BsgI	GTGCAG	2 cut(s)	1062, 1392
Bsh1236I	CGCG	2 cut(s)	50, 1200
BshFI	GGCC	2 cut(s)	93, 1350
BshTI	ACCGGT	1 cut(s)	243
BsiHKAI	GWGCWC	4 cut(s)	839, 964, 1076, 1323
BsiSI	CCGG	6 cut(s)	90, 244, 512, 812, 1351, 1444
BslI	CCNNNNNNNGG	3 cut(s)	100, 222, 250
BsmAI	GTCTC	1 cut(s)	248
BsmI	GAATGC	1 cut(s)	1398
BsnI	GGCC	2 cut(s)	93, 1350
Bso31I	GGTCTC	1 cut(s)	248
Bsp1286I	GDGCHC	5 cut(s)	364, 839, 964, 1076, 1323
Bsp1407I	TGTACA	1 cut(s)	1062
Bsp143I	GATC	5 cut(s)	562, 993, 1096, 1339, 1501
Bsp19I	CCATGG	1 cut(s)	1548
Bsp68I	TCGCGA	1 cut(s)	1200
BspACI	CCGC	6 cut(s)	50, 59, 166, 789, 1152, 1412
BspANI	GGCC	2 cut(s)	93, 1350
BspCNI	CTCAG	3 cut(s)	234, 277, 1309
BspFNI	CGCG	2 cut(s)	50, 1200
BspHI	TCATGA	2 cut(s)	619, 801
BspLI	GGNNCC	2 cut(s)	1426, 1503
BspMI	ACCTGC	2 cut(s)	959, 1082
BspPI	GGATC	4 cut(s)	557, 988, 1496, 1509
BspTI	CTTAAG	1 cut(s)	1304
BspTNI	GGTCTC	1 cut(s)	248
BsrBI	CCGCTC	1 cut(s)	1152
BsrDI	GCAATG	3 cut(s)	746, 947, 1380
BsrFI	RCCGGY	2 cut(s)	89, 243
BsrGI	TGTACA	1 cut(s)	1062
BsrI	ACTGG	2 cut(s)	547, 969
BssAI	RCCGGY	2 cut(s)	89, 243
BssECI	CCNNGG	2 cut(s)	99, 1548
BssMI	GATC	5 cut(s)	562, 993, 1096, 1339, 1501
BssT1I	CCWWGG	1 cut(s)	1548
Bst2UI	CCWGG	1 cut(s)	101
Bst4CI	ACNGT	1 cut(s)	233
Bst6I	CTCTTC	1 cut(s)	1106
BstAFI	CTTAAG	1 cut(s)	1304
BstAUI	TGTACA	1 cut(s)	1062
BstC8I	GCNNGC	5 cut(s)	33, 91, 166, 954, 1441
BstDEI	CTNAG	7 cut(s)	221, 285, 660, 792, 1221, 1317, 1580
BstDSI	CCRYGG	1 cut(s)	1548
BstENI	CCTNNNNNAGG	1 cut(s)	98
BstF5I	GGATG	2 cut(s)	520, 874
BstFNI	CGCG	2 cut(s)	50, 1200
BstHHI	GCGC	3 cut(s)	50, 64, 612
BstKTI	GATC	5 cut(s)	565, 996, 1099, 1342, 1504
BstMAI	GTCTC	1 cut(s)	248
BstMBI	GATC	5 cut(s)	562, 993, 1096, 1339, 1501
BstMWI	GCNNNNNNNGC	4 cut(s)	56, 137, 341, 953
BstNI	CCWGG	1 cut(s)	101
BstNSI	RCATGY	4 cut(s)	960, 1207, 1418, 1443
BstSCI	CCNGG	2 cut(s)	99, 510
BstSFI	CTRYAG	2 cut(s)	232, 1044
BstSLI	GKGCMC	1 cut(s)	964
BstUI	CGCG	2 cut(s)	50, 1200
BstV1I	GCAGC	3 cut(s)	1126, 1393, 1490
BstX2I	RGATCY	2 cut(s)	993, 1501
BstYI	RGATCY	2 cut(s)	993, 1501
BsuRI	GGCC	2 cut(s)	93, 1350
BtgI	CCRYGG	1 cut(s)	1548
BtsCI	GGATG	2 cut(s)	520, 874
BtsIMutI	CAGTG	2 cut(s)	936, 962
BtuMI	TCGCGA	1 cut(s)	1200
BveI	ACCTGC	2 cut(s)	959, 1082
Cac8I	GCNNGC	5 cut(s)	33, 91, 166, 954, 1441
CciI	TCATGA	2 cut(s)	619, 801
CfoI	GCGC	3 cut(s)	50, 64, 612
Cfr10I	RCCGGY	2 cut(s)	89, 243
Cfr13I	GGNCC	1 cut(s)	241
CseI	GACGC	1 cut(s)	640
Csp6I	GTAC	2 cut(s)	136, 1063
CspAI	ACCGGT	1 cut(s)	243
CviQI	GTAC	2 cut(s)	136, 1063
DdeI	CTNAG	7 cut(s)	221, 285, 660, 792, 1221, 1317, 1580
DpnI	GATC	5 cut(s)	564, 995, 1098, 1341, 1503
DpnII	GATC	5 cut(s)	562, 993, 1096, 1339, 1501
DrdI	GACNNNNNNGTC	1 cut(s)	1180
DriI	GACNNNNNGTC	1 cut(s)	261
DseDI	GACNNNNNNGTC	1 cut(s)	1180
EaeI	YGGCCR	1 cut(s)	1348
Eam1104I	CTCTTC	1 cut(s)	1106
Eam1105I	GACNNNNNGTC	1 cut(s)	261
EarI	CTCTTC	1 cut(s)	1106
Ecl136II	GAGCTC	1 cut(s)	837
Eco130I	CCWWGG	1 cut(s)	1548
Eco24I	GRGCYC	2 cut(s)	364, 839
Eco31I	GGTCTC	1 cut(s)	248
Eco32I	GATATC	1 cut(s)	1033
Eco47I	GGWCC	1 cut(s)	241
Eco53kI	GAGCTC	1 cut(s)	837
Eco57I	CTGAAG	2 cut(s)	359, 588
EcoICRI	GAGCTC	1 cut(s)	837
EcoNI	CCTNNNNNAGG	1 cut(s)	98
EcoRII	CCWGG	1 cut(s)	99
EcoRV	GATATC	1 cut(s)	1033
EcoT14I	CCWWGG	1 cut(s)	1548
EcoT22I	ATGCAT	1 cut(s)	748
EcoT38I	GRGCYC	2 cut(s)	364, 839
ErhI	CCWWGG	1 cut(s)	1548
FalI	AAGNNNNNCTT	2 cut(s)	78, 110
FauI	CCCGC	2 cut(s)	173, 1145
Fnu4HI	GCNGC	5 cut(s)	60, 789, 1140, 1407, 1479
FokI	GGATG	2 cut(s)	527, 881
FriOI	GRGCYC	2 cut(s)	364, 839
Fsp4HI	GCNGC	5 cut(s)	60, 789, 1140, 1407, 1479
FspBI	CTAG	2 cut(s)	365, 1011
GlaI	GCGC	3 cut(s)	49, 63, 611
GluI	GCNGC	5 cut(s)	60, 789, 1140, 1407, 1479
HaeIII	GGCC	2 cut(s)	93, 1350
HapII	CCGG	6 cut(s)	90, 244, 512, 812, 1351, 1444
HgaI	GACGC	1 cut(s)	640
HhaI	GCGC	3 cut(s)	50, 64, 612
Hin6I	GCGC	3 cut(s)	48, 62, 610
HinP1I	GCGC	3 cut(s)	48, 62, 610
HincII	GTYRAC	1 cut(s)	496
HindII	GTYRAC	1 cut(s)	496
HindIII	AAGCTT	1 cut(s)	1463
HinfI	GANTC	4 cut(s)	53, 934, 1447, 1486
HpaII	CCGG	6 cut(s)	90, 244, 512, 812, 1351, 1444
HphI	GGTGA	5 cut(s)	313, 572, 610, 998, 1106
Hpy166II	GTNNAC	3 cut(s)	496, 528, 962
Hpy188I	TCNGA	4 cut(s)	286, 339, 727, 1213
Hpy8I	GTNNAC	3 cut(s)	496, 528, 962
Hpy99I	CGWCG	1 cut(s)	182
HpyAV	CCTTC	4 cut(s)	334, 403, 977, 1256
HpyCH4III	ACNGT	1 cut(s)	233
HpyF10VI	GCNNNNNNNGC	4 cut(s)	56, 137, 341, 953
HpyF3I	CTNAG	7 cut(s)	221, 285, 660, 792, 1221, 1317, 1580
HspAI	GCGC	3 cut(s)	48, 62, 610
KroI	GCCGGC	1 cut(s)	89
KroNI	GCCGGC	1 cut(s)	91
Kzo9I	GATC	5 cut(s)	562, 993, 1096, 1339, 1501
LmnI	GCTCC	4 cut(s)	225, 834, 842, 1424
Lsp1109I	GCAGC	3 cut(s)	1126, 1393, 1490
LweI	GCATC	5 cut(s)	44, 162, 798, 1219, 1275
MaeI	CTAG	2 cut(s)	365, 1011
MaeIII	GTNAC	6 cut(s)	41, 538, 628, 851, 931, 1570
MalI	GATC	5 cut(s)	564, 995, 1098, 1341, 1503
MbiI	CCGCTC	1 cut(s)	1152
MboI	GATC	5 cut(s)	562, 993, 1096, 1339, 1501
MboII	GAAGA	8 cut(s)	409, 581, 817, 992, 1003, 1093, 1271, 1334
MfeI	CAATTG	2 cut(s)	65, 1185
MflI	RGATCY	2 cut(s)	993, 1501
MhlI	GDGCHC	5 cut(s)	364, 839, 964, 1076, 1323
MlyI	GAGTC	2 cut(s)	928, 1495
MmeI	TCCRAC	1 cut(s)	1479
MnlI	CCTC	8 cut(s)	3, 92, 232, 244, 287, 345, 472, 1465
Mph1103I	ATGCAT	1 cut(s)	748
MroNI	GCCGGC	1 cut(s)	89
MroXI	GAANNNNTTC	2 cut(s)	716, 1019
MseI	TTAA	5 cut(s)	290, 356, 615, 884, 1305
MslI	CAYNNNNRTG	2 cut(s)	513, 806
MspA1I	CMGCKG	1 cut(s)	1406
MspCI	CTTAAG	1 cut(s)	1304
MspI	CCGG	6 cut(s)	90, 244, 512, 812, 1351, 1444
MspR9I	CCNGG	2 cut(s)	101, 512
MunI	CAATTG	2 cut(s)	65, 1185
Mva1269I	GAATGC	1 cut(s)	1398
MvaI	CCWGG	1 cut(s)	101
MvnI	CGCG	2 cut(s)	50, 1200
MwoI	GCNNNNNNNGC	4 cut(s)	56, 137, 341, 953
NaeI	GCCGGC	1 cut(s)	91
NciI	CCSGG	1 cut(s)	512
NcoI	CCATGG	1 cut(s)	1548
NdeII	GATC	5 cut(s)	562, 993, 1096, 1339, 1501
NgoMIV	GCCGGC	1 cut(s)	89
NlaIV	GGNNCC	2 cut(s)	1426, 1503
NmuCI	GTSAC	3 cut(s)	628, 931, 1570
NruI	TCGCGA	1 cut(s)	1200
NsiI	ATGCAT	1 cut(s)	748
NspI	RCATGY	4 cut(s)	960, 1207, 1418, 1443
PaeI	GCATGC	1 cut(s)	1443
PagI	TCATGA	2 cut(s)	619, 801
PaqCI	CACCTGC	1 cut(s)	1082
PctI	GAATGC	1 cut(s)	1398
PdiI	GCCGGC	1 cut(s)	91
PdmI	GAANNNNTTC	2 cut(s)	716, 1019
PfeI	GAWTC	2 cut(s)	53, 1447
PinAI	ACCGGT	1 cut(s)	243
PkrI	GCNGC	5 cut(s)	61, 790, 1141, 1408, 1480
PleI	GAGTC	2 cut(s)	928, 1494
PpsI	GAGTC	2 cut(s)	928, 1494
PshBI	ATTAAT	1 cut(s)	615
Psp124BI	GAGCTC	1 cut(s)	839
Psp6I	CCWGG	1 cut(s)	99
PspGI	CCWGG	1 cut(s)	99
PspN4I	GGNNCC	2 cut(s)	1426, 1503
PspPI	GGNCC	1 cut(s)	241
PsuI	RGATCY	2 cut(s)	993, 1501
PvuII	CAGCTG	1 cut(s)	1406
RruI	TCGCGA	1 cut(s)	1200
RsaI	GTAC	2 cut(s)	137, 1064
RsaNI	GTAC	2 cut(s)	136, 1063
RseI	CAYNNNNRTG	2 cut(s)	513, 806
SacI	GAGCTC	1 cut(s)	839
SaqAI	TTAA	5 cut(s)	290, 356, 615, 884, 1305
SatI	GCNGC	5 cut(s)	60, 789, 1140, 1407, 1479
Sau3AI	GATC	5 cut(s)	562, 993, 1096, 1339, 1501
Sau96I	GGNCC	1 cut(s)	241
SchI	GAGTC	2 cut(s)	928, 1495
ScrFI	CCNGG	2 cut(s)	101, 512
SduI	GDGCHC	5 cut(s)	364, 839, 964, 1076, 1323
SfaNI	GCATC	5 cut(s)	44, 162, 798, 1219, 1275
SfcI	CTRYAG	2 cut(s)	232, 1044
SinI	GGWCC	1 cut(s)	241
SmiMI	CAYNNNNRTG	2 cut(s)	513, 806
SmlI	CTYRAG	1 cut(s)	1304
SmoI	CTYRAG	1 cut(s)	1304
SphI	GCATGC	1 cut(s)	1443
SsiI	CCGC	6 cut(s)	50, 59, 166, 789, 1152, 1412
SspMI	CTAG	2 cut(s)	365, 1011
SstI	GAGCTC	1 cut(s)	839
StyD4I	CCNGG	2 cut(s)	99, 510
StyI	CCWWGG	1 cut(s)	1548
TaaI	ACNGT	1 cut(s)	233
TaqI	TCGA	5 cut(s)	177, 650, 1023, 1269, 1535
TaqII	GACCGA	2 cut(s)	146, 639
TatI	WGTACW	1 cut(s)	1062
TauI	GCSGC	2 cut(s)	62, 791
TfiI	GAWTC	2 cut(s)	53, 1447
Tru1I	TTAA	5 cut(s)	290, 356, 615, 884, 1305
Tru9I	TTAA	5 cut(s)	290, 356, 615, 884, 1305
TscAI	CASTG	2 cut(s)	936, 969
TseFI	GTSAC	3 cut(s)	628, 931, 1570
TseI	GCWGC	3 cut(s)	1139, 1406, 1478
Tsp45I	GTSAC	3 cut(s)	628, 931, 1570
TspDTI	ATGAA	6 cut(s)	17, 318, 790, 818, 1014, 1082
TspGWI	ACGGA	2 cut(s)	480, 1182
TspRI	CASTG	2 cut(s)	936, 969
Vha464I	CTTAAG	1 cut(s)	1304
VneI	GTGCAC	1 cut(s)	960
VpaK11BI	GGWCC	1 cut(s)	241
VspI	ATTAAT	1 cut(s)	615
XagI	CCTNNNNNAGG	1 cut(s)	98
XapI	RAATTY	1 cut(s)	670
XceI	RCATGY	4 cut(s)	960, 1207, 1418, 1443
XmnI	GAANNNNTTC	2 cut(s)	716, 1019
XspI	CTAG	2 cut(s)	365, 1011
Zsp2I	ATGCAT	1 cut(s)	748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.