MD17G1057300.v1.1

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002976
Physical Location & Seq
Reverse (-)
4685217 .. 4687059
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1057300.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGCGACTGCGAAAACTGTGAAGGATGTTTCCCCTCACGAATTTGTCAAGGCTTACGCCGCCCATCTGAAGCGATCTGGAAAGGTTGAGCTTCCTCCATGGACTGATATCGTTAAGACTGGAAGATTCAAGGAGTTGGCTCCTTATGATCCTGACTGGTACTATGTCAGAGCTGCCTCCATGGCAAGGAAGATCTACTTGAGGGGAGGACTCGGTGTTGGTGCATTCCGTAGGATTTATGGTGGAAGCCAGAGGAATGGAAGTCGTCCACCACATTTCTGCGAGAGCAGTGGTGCTATTGCCCGACACATTCTCCAACAGTTACAGAAATTGAACATTGTTGATGTTGATCCTAAGGGGGGGAGGAGAATCACATCTAACGGTCAACGCGATCTTGACCAAGTTGCAGGCCGGATTGTGGTAACCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S19e

Protein Analysis

144

Amino Acids

15.9

Weight (kDa)

10.2

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S19e PF01090 6 - 140 4.5e-57 Ribosomal protein S19e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 390
AciI CCGC 1 cut(s) 60
AclWI GGATC 2 cut(s) 143, 344
AcsI RAATTY 1 cut(s) 41
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 3 cut(s) 186, 359, 418
AgsI TTSAA 2 cut(s) 130, 334
AluBI AGCT 2 cut(s) 91, 173
AluI AGCT 2 cut(s) 91, 173
AlwI GGATC 2 cut(s) 143, 344
AoxI GGCC 1 cut(s) 409
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 1 cut(s) 41
AxyI CCTNAGG 1 cut(s) 354
BbvI GCAGC 1 cut(s) 160
BccI CCATC 1 cut(s) 72
BglI GCCNNNNNGGC 1 cut(s) 182
BglII AGATCT 1 cut(s) 192
BisI GCNGC 2 cut(s) 60, 174
BlsI GCNGC 2 cut(s) 61, 175
BmiI GGNNCC 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 220
BsaBI GATNNNNATC 1 cut(s) 348
BsaJI CCNNGG 2 cut(s) 98, 180
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 3 cut(s) 186, 359, 418
Bse1I ACTGG 2 cut(s) 124, 161
Bse21I CCTNAGG 1 cut(s) 354
Bse8I GATNNNNATC 1 cut(s) 348
BseDI CCNNGG 2 cut(s) 98, 180
BseGI GGATG 1 cut(s) 31
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 3 cut(s) 186, 359, 418
BseNI ACTGG 2 cut(s) 124, 161
BseRI GAGGAG 1 cut(s) 379
BseXI GCAGC 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 1 cut(s) 411
BsiSI CCGG 1 cut(s) 412
BslI CCNNNNNNNGG 3 cut(s) 186, 359, 418
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 1 cut(s) 411
Bsp143I GATC 5 cut(s) 74, 148, 192, 349, 391
Bsp19I CCATGG 2 cut(s) 98, 180
BspACI CCGC 1 cut(s) 60
BspANI GGCC 1 cut(s) 411
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 1 cut(s) 141
BspPI GGATC 2 cut(s) 143, 344
BsrI ACTGG 2 cut(s) 124, 161
BssECI CCNNGG 2 cut(s) 98, 180
BssMI GATC 5 cut(s) 74, 148, 192, 349, 391
BssT1I CCWWGG 2 cut(s) 98, 180
Bst4CI ACNGT 3 cut(s) 19, 321, 383
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 2 cut(s) 98, 180
BstEII GGTNACC 1 cut(s) 421
BstF5I GGATG 1 cut(s) 31
BstFNI CGCG 1 cut(s) 390
BstKTI GATC 5 cut(s) 77, 151, 195, 352, 394
BstMBI GATC 5 cut(s) 74, 148, 192, 349, 391
BstMWI GCNNNNNNNGC 2 cut(s) 59, 182
BstPI GGTNACC 1 cut(s) 421
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 160
BstX2I RGATCY 1 cut(s) 192
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 1 cut(s) 192
Bsu36I CCTNAGG 1 cut(s) 354
BsuRI GGCC 1 cut(s) 411
BtgI CCRYGG 2 cut(s) 98, 180
BtsCI GGATG 1 cut(s) 31
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 409
Csp6I GTAC 1 cut(s) 160
CviAII CATG 3 cut(s) 99, 181, 429
CviJI RGCY 6 cut(s) 53, 91, 140, 173, 249, 411
CviKI_1 RGCY 6 cut(s) 53, 91, 140, 173, 249, 411
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 354
DpnI GATC 5 cut(s) 76, 150, 194, 351, 393
DpnII GATC 5 cut(s) 74, 148, 192, 349, 391
Eco130I CCWWGG 2 cut(s) 98, 180
Eco32I GATATC 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 89
Eco81I CCTNAGG 1 cut(s) 354
Eco91I GGTNACC 1 cut(s) 421
EcoO65I GGTNACC 1 cut(s) 421
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 98, 180
ErhI CCWWGG 2 cut(s) 98, 180
FaeI CATG 3 cut(s) 102, 184, 432
FaiI YATR 6 cut(s) 100, 147, 165, 182, 240, 430
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FatI CATG 3 cut(s) 98, 180, 428
Fnu4HI GCNGC 2 cut(s) 60, 174
FokI GGATG 1 cut(s) 38
Fsp4HI GCNGC 2 cut(s) 60, 174
GluI GCNGC 2 cut(s) 60, 174
HaeIII GGCC 1 cut(s) 411
HapII CCGG 1 cut(s) 412
Hin1II CATG 3 cut(s) 102, 184, 432
HincII GTYRAC 1 cut(s) 386
HindII GTYRAC 1 cut(s) 386
HinfI GANTC 3 cut(s) 126, 210, 369
HpaII CCGG 1 cut(s) 412
Hpy166II GTNNAC 2 cut(s) 269, 386
Hpy188I TCNGA 2 cut(s) 69, 170
Hpy188III TCNNGA 4 cut(s) 38, 78, 152, 395
Hpy8I GTNNAC 2 cut(s) 269, 386
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 3 cut(s) 19, 321, 383
HpyCH4V TGCA 2 cut(s) 224, 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 182
HpyF3I CTNAG 1 cut(s) 354
Hsp92II CATG 3 cut(s) 102, 184, 432
Kzo9I GATC 5 cut(s) 74, 148, 192, 349, 391
LmnI GCTCC 1 cut(s) 145
LpnPI CCDG 7 cut(s) 63, 105, 142, 165, 263, 393, 425
Lsp1109I GCAGC 1 cut(s) 160
MaeIII GTNAC 2 cut(s) 321, 421
MalI GATC 5 cut(s) 76, 150, 194, 351, 393
MboI GATC 5 cut(s) 74, 148, 192, 349, 391
MboII GAAGA 2 cut(s) 135, 202
MflI RGATCY 1 cut(s) 192
MluCI AATT 2 cut(s) 41, 329
MlyI GAGTC 1 cut(s) 204
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 7 cut(s) 45, 105, 187, 195, 200, 246, 357
MseI TTAA 1 cut(s) 114
MspI CCGG 1 cut(s) 412
Mva1269I GAATGC 1 cut(s) 224
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 2 cut(s) 59, 182
NcoI CCATGG 2 cut(s) 98, 180
NdeII GATC 5 cut(s) 74, 148, 192, 349, 391
NlaIII CATG 3 cut(s) 102, 184, 432
NlaIV GGNNCC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 1 cut(s) 387
PctI GAATGC 1 cut(s) 224
PfeI GAWTC 2 cut(s) 126, 369
PkrI GCNGC 2 cut(s) 61, 175
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
PspEI GGTNACC 1 cut(s) 421
PspN4I GGNNCC 1 cut(s) 141
PsuI RGATCY 1 cut(s) 192
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 2 cut(s) 60, 174
Sau3AI GATC 5 cut(s) 74, 148, 192, 349, 391
SchI GAGTC 1 cut(s) 204
SetI ASST 3 cut(s) 87, 93, 175
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 2 cut(s) 41, 329
SsiI CCGC 1 cut(s) 60
StyI CCWWGG 2 cut(s) 98, 180
TaaI ACNGT 3 cut(s) 19, 321, 383
TasI AATT 2 cut(s) 41, 329
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 2 cut(s) 126, 369
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 1 cut(s) 295
TseI GCWGC 1 cut(s) 173
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 1 cut(s) 295
XapI RAATTY 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.