Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004656

Physical Location & Seq

Genomic Coordinates Forward (+)

5522220 .. 5523320

Gene Structure 1101 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1068100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1101 bp

ATGACTGAGCTCTCCGCAACCACTCAGCTACCCGGCGACCCACAACCAACAAATGAACCCGTTGACCCGCAAACCCACCCAACCCTCCTCATCGATCCCCTCACCCACTGCTGCGCCAATCACAACTGCAACCCCTTCGCCGTCGATGCTCTTGATTCCCGGAGCACCTCCGATCGAAACTGGTTCTCTGATGCCGCCTCCAATTCTGACCATGACGTCAGCTGGTTGGTCACCGATCTTTTCGACCGCAGCAGCTCGGAGCAGCAGCGCAATACCGACTGCGTTGTCGATTCGGGTTCGCGGTCGGATCCGTTTGCGACCGAATTGGAAATTGGGGGTGTTGAGGACGGCGACGAAACGGAGTCGAATTTAGGGTTTGGGTCAAACTCTATGGGATCGGTTTCTGAGCTTCGGATTGTTGGGTTGGAGTTGGAATCGGAATCGGATTCGGGTACAATCGATGACATAGAAAGTGATCGGATCACCGATTCGGGTATCCAGTCAATTTGGGATAACCTTTTCTTGATGGAGCAGAGGAATATGCTTGAAAGTTCTGAATCGAACGAGAGTAATCCGGTCAACGGCGGAGTGGAGGAGCTATCAGTGGTCTCTGGGTTTTTCACCGCGGAAGCCGAGGTTCTCCAAGGAGCAGCACGAAGCATGGAGTGGGAAATAGTCAACAATTTTCGGAGGTTCGCGGATGATGACGATCTTGACTTAGAGACTGCAGAGTACGTCAACACGATTTTCGGGCAATTCGTGGAGAATGCCAATGCCATGAATGGCCGTCCTCCGGCAGCTTTAAGCGTGGTCGAAGATCTTCCATTGGTAGAGTTTACAGTCGAAGAATTGAAGAAAGAAGAAGCAGTTTGTGCTGTTTGTAAAGACAGCATTTTGGTGGAGGATAAAGTCAGAAGCTTGCCCTGCTGCCATTACTACCATCATGATTGCATTGTGCCGTGGTTGGGCATTCGGAATACATGCCCTGTTTGTAGGTACGAGCTGCCCACCGATGATCCCGATTACGAAAGGAGCAAGCGTGAAAGTGCTGCTCGTGGTGAGCCGGGGGATTTACAAGTCAGGTTTGAACAGTTTGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009987 GO:0010026 GO:0010154 GO:0016043 GO:0016049 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022412 GO:0022414 GO:0030154 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032989 GO:0036211 GO:0040007 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048316 GO:0048468 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0060560 GO:0061458 GO:0070647 GO:0071704 GO:0071840 GO:0090376 GO:0090378 GO:0090558 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

367

Amino Acids

40.55

Weight (kDa)

4.12

Isoelectric Point (pI)

51.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	291 - 332	1.3e-11	Ring finger domain
zf-C3HC4_2	PF13923	291 - 331	1.4e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	291 - 318	1.6e-06	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004656)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02340 AT3G02340 AT5G15820
fragaria_vesca	FvH4_6g46010 FvH4_6g46010 FvH4_6g46010
malus_domestica	MD09G1078100.v1.1 MD17G1068100.v1.1 MD17G1068500.v1.1
prunus_persica	Prupe.3G246300_v2.0.a1
pyrus_communis	pycom09g00500 pycom17g06870 pycom17g06880
rosa_chinensis	RchiOBHm_Chr2g0164241
rosa_laevigata	RLG00000021448
rosa_multiflora	Rmu_sc0000854.1_g000011 Rmu_sc0001427.1_g000007 Rmu_sc0004956.1_g000001 Rmu_sc0007154.1_g000003 Rmu_sc0008593.1_g000002 Rmu_sc0018459.1_g000001 Rmu_sc0041118.1_g000003
rosa_roxburghii	Rroxscaffold_2G00086690 Rroxscaffold_6G00413980
rosa_rugosa	Rorug02G0507400 Rorug07G0301000
rosa_samantha	Rh2AG575600 Rh2BG586400 Rh2CG557200 Rh2DG596000
rosa_wichuraiana	Rw2G047650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	215, 284
AatII	GACGTC	1 cut(s)	219
AccII	CGCG	3 cut(s)	301, 626, 698
AciI	CCGC	9 cut(s)	15, 68, 195, 247, 301, 585, 624, 626, 698
AclWI	GGATC	6 cut(s)	89, 302, 315, 403, 488, 1010
AcoI	YGGCCR	1 cut(s)	784
AcsI	RAATTY	1 cut(s)	367
AcyI	GRCGYC	1 cut(s)	216
AfaI	GTAC	3 cut(s)	454, 734, 998
AfiI	CCNNNNNNNGG	3 cut(s)	581, 793, 965
AgsI	TTSAA	3 cut(s)	548, 853, 1088
AjuI	GAANNNNNNNTTGG	2 cut(s)	315, 347
AluBI	AGCT	9 cut(s)	10, 28, 222, 255, 409, 598, 800, 918, 1003
AluI	AGCT	9 cut(s)	10, 28, 222, 255, 409, 598, 800, 918, 1003
Alw21I	GWGCWC	2 cut(s)	12, 167
Alw26I	GTCTC	2 cut(s)	613, 716
AlwI	GGATC	6 cut(s)	89, 302, 315, 403, 488, 1010
AoxI	GGCC	1 cut(s)	784
ApoI	RAATTY	1 cut(s)	367
Asp700I	GAANNNNTTC	1 cut(s)	819
AspLEI	GCGC	2 cut(s)	116, 270
AsuC2I	CCSGG	3 cut(s)	33, 160, 1065
AsuHPI	GGTGA	5 cut(s)	94, 223, 475, 613, 1070
BamHI	GGATCC	1 cut(s)	307
BanII	GRGCYC	1 cut(s)	12
BauI	CACGAG	1 cut(s)	1053
Bbv12I	GWGCWC	2 cut(s)	12, 167
BccI	CCATC	2 cut(s)	520, 948
BceAI	ACGGC	5 cut(s)	125, 364, 598, 771, 943
BcgI	CGANNNNNNTGC	2 cut(s)	118, 152
BciVI	GTATCC	1 cut(s)	506
BcnI	CCSGG	3 cut(s)	33, 160, 1065
BcoDI	GTCTC	2 cut(s)	613, 716
BfmI	CTRYAG	1 cut(s)	726
BfuI	GTATCC	1 cut(s)	506
BglII	AGATCT	1 cut(s)	817
Bme1390I	CCNGG	3 cut(s)	33, 160, 1065
BmiI	GGNNCC	1 cut(s)	309
BmrFI	CCNGG	3 cut(s)	33, 160, 1065
BmsI	GCATC	2 cut(s)	136, 181
BpuMI	CCSGG	3 cut(s)	33, 160, 1065
Bsa29I	ATCGAT	2 cut(s)	93, 459
BsaBI	GATNNNNATC	1 cut(s)	708
BsaHI	GRCGYC	1 cut(s)	216
BsaI	GGTCTC	1 cut(s)	613
BsaJI	CCNNGG	5 cut(s)	624, 633, 643, 959, 1064
BsaWI	WCCGGW	1 cut(s)	574
BsaXI	ACNNNNNCTCC	2 cut(s)	1024, 1054
Bsc4I	CCNNNNNNNGG	3 cut(s)	581, 793, 965
Bse1I	ACTGG	2 cut(s)	185, 499
Bse8I	GATNNNNATC	1 cut(s)	708
BseCI	ATCGAT	2 cut(s)	93, 459
BseDI	CCNNGG	5 cut(s)	624, 633, 643, 959, 1064
BseGI	GGATG	1 cut(s)	706
BseJI	GATNNNNATC	1 cut(s)	708
BseLI	CCNNNNNNNGG	3 cut(s)	581, 793, 965
BseMII	CTCAG	2 cut(s)	38, 396
BseNI	ACTGG	2 cut(s)	185, 499
BseRI	GAGGAG	2 cut(s)	77, 608
Bsh1236I	CGCG	3 cut(s)	301, 626, 698
Bsh1285I	CGRYCG	4 cut(s)	175, 247, 305, 321
BshFI	GGCC	1 cut(s)	786
BshVI	ATCGAT	2 cut(s)	93, 459
BsiEI	CGRYCG	4 cut(s)	175, 247, 305, 321
BsiHKAI	GWGCWC	2 cut(s)	12, 167
BsiSI	CCGG	5 cut(s)	33, 160, 575, 794, 1064
BslI	CCNNNNNNNGG	3 cut(s)	581, 793, 965
BsmAI	GTCTC	2 cut(s)	613, 716
BsmI	GAATGC	2 cut(s)	772, 969
BsnI	GGCC	1 cut(s)	786
Bso31I	GGTCTC	1 cut(s)	613
Bsp1286I	GDGCHC	2 cut(s)	12, 167
BspACI	CCGC	9 cut(s)	15, 68, 195, 247, 301, 585, 624, 626, 698
BspANI	GGCC	1 cut(s)	786
BspCNI	CTCAG	2 cut(s)	37, 397
BspDI	ATCGAT	2 cut(s)	93, 459
BspFNI	CGCG	3 cut(s)	301, 626, 698
BspHI	TCATGA	1 cut(s)	943
BspLI	GGNNCC	1 cut(s)	309
BspMAI	CTGCAG	1 cut(s)	730
BspPI	GGATC	6 cut(s)	89, 302, 315, 403, 488, 1010
BspTNI	GGTCTC	1 cut(s)	613
BsrI	ACTGG	2 cut(s)	185, 499
BssECI	CCNNGG	5 cut(s)	624, 633, 643, 959, 1064
BssNI	GRCGYC	1 cut(s)	216
BssSI	CACGAG	1 cut(s)	1053
BssT1I	CCWWGG	1 cut(s)	643
Bst2BI	CACGAG	1 cut(s)	1053
Bst4CI	ACNGT	2 cut(s)	841, 1092
BstACI	GRCGYC	1 cut(s)	216
BstAPI	GCANNNNNTGC	1 cut(s)	872
BstC8I	GCNNGC	2 cut(s)	920, 1037
BstDEI	CTNAG	4 cut(s)	6, 24, 405, 718
BstDSI	CCRYGG	2 cut(s)	624, 959
BstEII	GGTNACC	1 cut(s)	229
BstF5I	GGATG	1 cut(s)	706
BstFNI	CGCG	3 cut(s)	301, 626, 698
BstHHI	GCGC	2 cut(s)	116, 270
BstMAI	GTCTC	2 cut(s)	613, 716
BstMCI	CGRYCG	4 cut(s)	175, 247, 305, 321
BstMWI	GCNNNNNNNGC	3 cut(s)	146, 872, 924
BstNSI	RCATGY	1 cut(s)	984
BstPI	GGTNACC	1 cut(s)	229
BstSCI	CCNGG	3 cut(s)	31, 158, 1063
BstSFI	CTRYAG	1 cut(s)	726
BstUI	CGCG	3 cut(s)	301, 626, 698
BstX2I	RGATCY	2 cut(s)	307, 817
BstYI	RGATCY	2 cut(s)	307, 817
Bsu15I	ATCGAT	2 cut(s)	93, 459
BsuI	GTATCC	1 cut(s)	506
BsuRI	GGCC	1 cut(s)	786
BsuTUI	ATCGAT	2 cut(s)	93, 459
BtgI	CCRYGG	2 cut(s)	624, 959
BtsCI	GGATG	1 cut(s)	706
BtsI	GCAGTG	1 cut(s)	106
BtsIMutI	CAGTG	2 cut(s)	106, 609
Cac8I	GCNNGC	2 cut(s)	920, 1037
CciI	TCATGA	1 cut(s)	943
CfoI	GCGC	2 cut(s)	116, 270
Cfr42I	CCGCGG	1 cut(s)	627
ClaI	ATCGAT	2 cut(s)	93, 459
Csp6I	GTAC	3 cut(s)	453, 733, 997
CviAII	CATG	5 cut(s)	212, 661, 778, 944, 981
CviQI	GTAC	3 cut(s)	453, 733, 997
DdeI	CTNAG	4 cut(s)	6, 24, 405, 718
DrdI	GACNNNNNNGTC	2 cut(s)	215, 284
DseDI	GACNNNNNNGTC	2 cut(s)	215, 284
EaeI	YGGCCR	1 cut(s)	784
EciI	GGCGGA	1 cut(s)	600
Ecl136II	GAGCTC	1 cut(s)	10
Eco130I	CCWWGG	1 cut(s)	643
Eco24I	GRGCYC	1 cut(s)	12
Eco31I	GGTCTC	1 cut(s)	613
Eco53kI	GAGCTC	1 cut(s)	10
Eco91I	GGTNACC	1 cut(s)	229
EcoICRI	GAGCTC	1 cut(s)	10
EcoO65I	GGTNACC	1 cut(s)	229
EcoT14I	CCWWGG	1 cut(s)	643
EcoT38I	GRGCYC	1 cut(s)	12
ErhI	CCWWGG	1 cut(s)	643
FaeI	CATG	5 cut(s)	215, 664, 781, 947, 984
FaiI	YATR	8 cut(s)	213, 392, 467, 542, 662, 779, 945, 982
FatI	CATG	5 cut(s)	211, 660, 777, 943, 980
FauI	CCCGC	1 cut(s)	75
FokI	GGATG	1 cut(s)	713
FriOI	GRGCYC	1 cut(s)	12
GlaI	GCGC	2 cut(s)	115, 269
HaeIII	GGCC	1 cut(s)	786
HapII	CCGG	5 cut(s)	33, 160, 575, 794, 1064
HhaI	GCGC	2 cut(s)	116, 270
Hin1I	GRCGYC	1 cut(s)	216
Hin1II	CATG	5 cut(s)	215, 664, 781, 947, 984
Hin6I	GCGC	2 cut(s)	114, 268
HinP1I	GCGC	2 cut(s)	114, 268
HincII	GTYRAC	4 cut(s)	64, 580, 679, 739
HindII	GTYRAC	4 cut(s)	64, 580, 679, 739
HindIII	AAGCTT	1 cut(s)	916
HinfI	GANTC	8 cut(s)	155, 290, 362, 434, 440, 446, 488, 557
HpaII	CCGG	5 cut(s)	33, 160, 575, 794, 1064
HphI	GGTGA	5 cut(s)	94, 223, 475, 613, 1070
Hpy166II	GTNNAC	5 cut(s)	64, 580, 679, 739, 837
Hpy188III	TCNNGA	5 cut(s)	152, 523, 713, 944, 1019
Hpy8I	GTNNAC	5 cut(s)	64, 580, 679, 739, 837
Hpy99I	CGWCG	2 cut(s)	146, 356
HpyAV	CCTTC	1 cut(s)	145
HpyCH4III	ACNGT	2 cut(s)	841, 1092
HpyCH4IV	ACGT	2 cut(s)	216, 735
HpyCH4V	TGCA	3 cut(s)	129, 728, 951
HpyF10VI	GCNNNNNNNGC	3 cut(s)	146, 872, 924
HpyF3I	CTNAG	4 cut(s)	6, 24, 405, 718
HpySE526I	ACGT	2 cut(s)	216, 735
Hsp92I	GRCGYC	1 cut(s)	216
Hsp92II	CATG	5 cut(s)	215, 664, 781, 947, 984
HspAI	GCGC	2 cut(s)	114, 268
KspI	CCGCGG	1 cut(s)	627
LmnI	GCTCC	6 cut(s)	162, 259, 529, 595, 647, 1032
LweI	GCATC	2 cut(s)	136, 181
MaeII	ACGT	2 cut(s)	216, 735
MaeIII	GTNAC	1 cut(s)	229
MboII	GAAGA	5 cut(s)	812, 827, 857, 865, 872
MflI	RGATCY	2 cut(s)	307, 817
MhlI	GDGCHC	2 cut(s)	12, 167
MluCI	AATT	8 cut(s)	202, 323, 330, 367, 504, 682, 755, 848
MlyI	GAGTC	1 cut(s)	371
MmeI	TCCRAC	3 cut(s)	285, 405, 411
MroXI	GAANNNNTTC	1 cut(s)	819
MseI	TTAA	1 cut(s)	803
MslI	CAYNNNNRTG	1 cut(s)	896
MspA1I	CMGCKG	2 cut(s)	222, 626
MspI	CCGG	5 cut(s)	33, 160, 575, 794, 1064
MspR9I	CCNGG	3 cut(s)	33, 160, 1065
Mva1269I	GAATGC	2 cut(s)	772, 969
MvnI	CGCG	3 cut(s)	301, 626, 698
MwoI	GCNNNNNNNGC	3 cut(s)	146, 872, 924
NciI	CCSGG	3 cut(s)	33, 160, 1065
NlaIII	CATG	5 cut(s)	215, 664, 781, 947, 984
NlaIV	GGNNCC	1 cut(s)	309
NmeAIII	GCCGAG	1 cut(s)	658
NmuCI	GTSAC	1 cut(s)	229
NspI	RCATGY	1 cut(s)	984
PagI	TCATGA	1 cut(s)	943
PcsI	WCGNNNNNNNCGW	1 cut(s)	756
PctI	GAATGC	2 cut(s)	772, 969
PdmI	GAANNNNTTC	1 cut(s)	819
PfeI	GAWTC	7 cut(s)	155, 290, 434, 440, 446, 488, 557
PfoI	TCCNGGA	1 cut(s)	158
Ple19I	CGATCG	1 cut(s)	175
PleI	GAGTC	1 cut(s)	370
PpsI	GAGTC	1 cut(s)	370
Psp124BI	GAGCTC	1 cut(s)	12
PspEI	GGTNACC	1 cut(s)	229
PspN4I	GGNNCC	1 cut(s)	309
PstI	CTGCAG	1 cut(s)	730
PsuI	RGATCY	2 cut(s)	307, 817
PvuI	CGATCG	1 cut(s)	175
PvuII	CAGCTG	1 cut(s)	222
RsaI	GTAC	3 cut(s)	454, 734, 998
RsaNI	GTAC	3 cut(s)	453, 733, 997
RseI	CAYNNNNRTG	1 cut(s)	896
SacI	GAGCTC	1 cut(s)	12
SacII	CCGCGG	1 cut(s)	627
SaqAI	TTAA	1 cut(s)	803
SchI	GAGTC	1 cut(s)	371
ScrFI	CCNGG	3 cut(s)	33, 160, 1065
SduI	GDGCHC	2 cut(s)	12, 167
SfaNI	GCATC	2 cut(s)	136, 181
SfcI	CTRYAG	1 cut(s)	726
Sfr303I	CCGCGG	1 cut(s)	627
SgrBI	CCGCGG	1 cut(s)	627
SmiMI	CAYNNNNRTG	1 cut(s)	896
Sse9I	AATT	8 cut(s)	202, 323, 330, 367, 504, 682, 755, 848
SsiI	CCGC	9 cut(s)	15, 68, 195, 247, 301, 585, 624, 626, 698
SstI	GAGCTC	1 cut(s)	12
StyD4I	CCNGG	3 cut(s)	31, 158, 1063
StyI	CCWWGG	1 cut(s)	643
TaaI	ACNGT	2 cut(s)	841, 1092
TaiI	ACGT	2 cut(s)	219, 738
TaqII	GACCGA	1 cut(s)	335
TasI	AATT	8 cut(s)	202, 323, 330, 367, 504, 682, 755, 848
TauI	GCSGC	1 cut(s)	197
TfiI	GAWTC	7 cut(s)	155, 290, 434, 440, 446, 488, 557
Tru1I	TTAA	1 cut(s)	803
Tru9I	TTAA	1 cut(s)	803
TscAI	CASTG	2 cut(s)	113, 609
TseFI	GTSAC	1 cut(s)	229
Tsp45I	GTSAC	1 cut(s)	229
TspDTI	ATGAA	2 cut(s)	69, 794
TspGWI	ACGGA	2 cut(s)	300, 374
TspRI	CASTG	2 cut(s)	113, 609
XapI	RAATTY	1 cut(s)	367
XceI	RCATGY	1 cut(s)	984
XmnI	GAANNNNTTC	1 cut(s)	819
ZraI	GACGTC	1 cut(s)	217

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.