Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0013012

Physical Location & Seq

Genomic Coordinates Forward (+)

7563670 .. 7574912

Gene Structure 11243 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1091000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7386 bp

ATGGGCGATGGAGGCGTCACATGCATGCCTTTGCAGCATAATATCATGGACAGGTTTCCAATTCAGGAGAAGACTACTGTTTGCGGAGGCAAGAACGGTAACAATGGATTCAATTCCAAGACGGTTAAGAAGAAGAAGATAGTGAAGGTAATGAAGCCCAAGAAGAAAGTTGTCAAGAACCCTGTGTCTTCAAAGAACGTGGAGTCCGAAAAAAGTGAACTGGGATTGGATAAAGGTGCTAGCAGTGCGACTAAGGAAGCTGAGAATGGTGAAATTGCTGAGGAGAAGGAGGAAGTGGAAGAGGGTGAATTGGGGACTTTGAAGTGGCCCAAAGTTGAAGAAGAGAATGGTGAATTTGTGCCAGAGAAGTCGAGAAGAAGTGAGATTGAGAAGGGAGAGATTGTTGGTGAGAAGTGCAGGAGAAGTGAGGTGGAGAAAGCAGAGTTCCTTTCAGGGAAATGGCGAAGAGGGGACATAGAGAAAGGAGAGGTTGTTCCAGAGAGGAGCAGAAAAGTCGAAGCCGAATTCGGGTCCTGGAGACCTCCCAAGGATGATATTGAGAAAGGTGAGTTCATTCCAGATAGATGGCAGAAAGGGGAAGGGGCCAGAGATGACTACACTCCCAGTAAATTCCGCAGGTATGATATTGGTAAGGACAGGAGCTGGAAATTTGATCGTGAGCGTACGCCACCTTCGGGGAAGTATTCAAATGATGACCCTTTTAGAAGGAAAGAAAATAGAAGTGGAAGCCAGCAGATCAAGAGTATTGCCAGGTGGGAGAGTGGCCTTGACAGGAATACGAGGATCAGTTCCAAAATTGTTGATGAGGACAGTGCGTACAGAAATGAGTACAGCAATGGTAAGTGCCACCCCAGAGACTACCCTTCTATTAATCGGTTAAAAAGGTTTGGTACTGATACAAGTATCGGTGAGCGAAAGAATTATGGAGACTATGGGGATTATCCAGGTGCAAAATTTCGTAGGGTTTCTGATGACACCAATCGCTCTGCCCACCCTGAGCATTATTCACGCAGTTCTGTGGAGAGGTCTTATCGGAATCCTTCATCATCAAGAGTTGCAGCAGACAAGTACTCCTCCAGGCCTTATGAATCTACTTTGTCTTCCAGGGTAGTTTATGACAGGCATGGGAGAAGTCCTGGACTTCCAGGTCATTCTGAGCGATCCCCACATGATCGGGCCCGTTATTTTGATCACCGGGACAGAAGCCCATTGCGCCGTGAAAGATCTCCATATGTCCATGAGAGGTTTCCATATGGTCGGGAGAAATCACCGCATGGCCGTGAGAGATCACCGTATGTTCGTGAAAAATCACCGTATAGTCGTGAGAAATCACCACATGGCCGTGAGAAATCACCACATGGCCGGGAGAGATCACCATATGGCCGCGAGAAATCACCATTCAGGCGCGAGAGATCACCATATGGACGAGAGAGATCACCTTATGGTCGCGAGAGATCTCCATATGACAGGAGCCATCAATACGATCACAGGAACCGGAGTCTGTCTCCACACGATCGACCTCGATACCATGACCGCAGGAATCGCACTCCAAATTATTTGGAACGGTCCCCTCATGATCGGAGTAGACCTAATAATCATCGAGACACAAGTCGAAAAAGTGGAGCAAGTGAAAGGCGCAACTCCCGTCACGGTAATAGGGGGCAAGAAGATAAACCGGTGCAGAAGGATCCCTGTGGAAAAGACTCACATTCGACTGCTAAAGAATCTCTAGATAGAAGCACTGTGCCTGATGTTAATGTATCTGTGGAGACAAATTCCAACTGTGAGTCTCACAAAGAAGAACCGTCTCAAATTCCAAGTGTAAATTGCACCGAAAATTCTCATATCAGTGTAGCCCCTCCTGCAGAGCTGCTTTCGATGGAAGAAGATATGGATATATGTGATACACCACCACATGTCCCGGTAATTGCTGATTCATCCGCCGGGAAATGGTTTTACCTCGATTATTATGGTGTGGAACGTGGACCTTCAAAATTGTGTGAGCTTAAAGCACTTGTTGAAGAAGGGGCTCTTGTGTCTGACCACATGGTCAAGCACTCGGATAGTGATAGGTGGGTAACTGTTGAAAATGCAGTTTCACCACTGGTGACCATACATTTCCCATCCCTTGTATCAGACTCTATAACTCGACTAGTAAGCCCTCCAGAAGCTCCTGGTAATCTATTGGCAGATACTGGAGATACTGCGCAATATGATGCTCAGAGTGGGAAGGACACAGCAATCACTTTGCTGCCGCCAGGATTTGGCGCAGATGTTGGTGGAGCTGCTTCTGAGCCTTTAGAAGATCTCCATATTGAAGAAAGGGTTGCAGCTCTGATGGAGGGTTTTGCGGTTATTCCTGGCAGGGAGCTTGAAGCTGTGGGAGAAGTTCTGCAAATGTCATTTGAATATGCACAACGAGATGGATGGGAAAACACTGCAGGTTTCAGTCAAGGCCACGATGCTGAACTGTATGATCAGAAAACCGAGGAACCCGGCTATTCTGACATTAAAATAAAGGAAGCTGCAGAAATCTGGCTGACTGCACCTTCTGACAAGGACGCTGGTTTTGCTTGTGGTGATTCTGATGACTGGTTTTCTGATCGGTGGTCATGCAAGGGTGGTGATTGGAAGAGGAATGAAGAAGCTTCACAAGAGAGGTCTTCTAGGAAGAAATTTGTTGTCAATTATGGTTTCCCATTATGCCAGATGCCAAAGTCTGGTTATGAAGACCCTCGATGGCATAGAAAAGATGAATTGTATTATCCTTCGCAAAGTAGAAGACTTGATCTACCTACTTGGGGTTTTTCATGTCCAGGTGAGATAAATGATTGTAGTGGCGTGAGCAGAACAACTCAAGTTAAGCCTACTGTCATAAAAGGAACTATGCTTCCGGTAGTAAGGATAAATGCATGCGTGGTTAAAGACCATGGTTCATTTGTTTCTGAGCCTCGCATAAAAACCCGTGGTATGGAGAGGTACACGTCAAGGTCTTCTCGGTCATATTCTTCAGGTAGTGATGGGAAGAGATCATCGGGAGAAGGTGACACCCAGTTGAAACCAGTTGGTGAACGACGGTCACAGGGCTCTTCAAAATGCATTACGTCTATTAACACCAACAAAGACCGTATTTGCACAGTCGATGAATTGAAATTGCACTTGGGTGACTGGTACTACCTGGATGGTGCTGGGCACGAACAAGGACCTTCATCATTTTCTGAGCTACAGGTCTTGGTTGATCAAGGAGTGATCTTAAAACATAGTAGTGTTTTCCGGAAATTTGATAAAGTCTGGGTTCCTGTGACATCTGCTGCAGAGACTTCTGAAGCTACTCATATGAATCAGCATGAGAAGAACACAAGATCTAGTGATACTTCAGGACCTGCTCCTTCACAATCTAAAAATGCTTTATTTGAAGAATCGAGCTCAAAGTCGAGTTGGTTGCACAACCTGCATCCTCAATTCATTGGTTATACGTGCGGAAAGCTTCATGAACTGGTGATGAAATCTTACAAAAGCCGGGAGTTTGCTGCGGCCATAAATGATGTTCTAGATCCATGGATTAATGCAAAGCAGCCAAAGAAAGAGGTGGAGAAGCAAATGTACTGGAAAACAGATGGTGATGCACGTAATGCCAAAAGAGCCCGGTTGCTGATTGATGAAAGTGAAGAAGACTATGACATGGGAGATGATTTGCTAACAGTTGAAAAGGATGAGTCTACATTTGAGGATCTATGTGGTGATGCTTCTTTCTACAGAGAAAATAGTGGGAGTTATGGCTCAGAGATGGGAAGCTGGGGTTTATTAGACGGGCAAGTGCTGGCACGAGTCTTTCATTTTCTGAGATTAGACATGAATTCCCTTTCCTTTGCTTCTTTGACCTGTAAGCACTGGAGAGCTGCTGTCATGTTTTATAAGGATATTTCAAGACAGGTCGATTTTTCATCCTTGGGTCCTAACTGCACTGATTCAGCGATTGTGAACATTATGAGTGGTTATGGTAAAGAAAAGATTAACTCTATGGTTCTGATTGGTTGCACCAACATCACCCCCCGCACTCTTGAAGAGATCCTTAGTTCACTTCCTTGTTTATCTACCATAGATATCAGAGGCTGCAACCAGTTTGGCGAGTTGGTCATCAAGTTTCAGAATCTGAACTGGATTAAGAGCCGAAGTTCATCTGGTACGAAGATATTTGAGGAATCCTACTCGAAAATTAGGAGTCTAAAGCAGATCAGTGAGAAATCTTCATCTGTTTCTAGAAGTAAGGTTCTAGGCAATGATATGGATGATTTTAGTGAACTGAAAGTGTACTTTGATAGTGTGGATAAGAGAGAAACTGCAAATCTATCATTCCGGGGAAGTTTGTACAAACGTTCAAAACTATTTGATGCTAGACGGTCCTCATCCATTCTATCTAGGGATGCTCGCATGAGGCGGTTGTCCATTAAGAAATCTGAACACGGTTACAAGAAGATTGAGGAATTTGTTGCTTCAAGTCTGAAGGATATCATGAAAGACAATCCCTATGATTTCTTTGTGCCCAAGGTTGCTGAAATTCAGGATAAAATGAGAAATGGTCATTACATACGCCGAGGATTGAGTTCTGTCAAGGAGGATATCAGTCGAATGTGCAGGGATGCAATAAAAGCAAAGAATCGGGGGGATGCTGGAGACATGAATCACATTATAACATTGTTTATTCAACTTGCTACACGTTTAGAAGTGGCTACTAAGTCTTCTAATGAAAGAGATGAGCTGATTAAGTCATGGGAAGATGATACATTTGCAGGGTTCTCTTCATCCTCCAAGTGTAGAAAGAAGATCAATAAAGTAGCACCTGAAAGGAAGTACTCGAATAGGAGTAATGGCACTGTGAATGGTAGTTTGGATTATGGAGAGTATGCATCTGATCGAGAAATCAGAAGGCGTTTATCCAGATTGAATAAAAAATCTATGGATTCAGAAAGTGAAACCTCTGATGATATGGACAAGTCTTCTGAATATAGCAAGAGCAATAGTGATAGTACTTCTTCAGATACAGAAAGTGACTCCGAAGTGAGGTCACAAAGTCAAACTGGGCAGTCAAGAGCAGATGGAAGCTATACACCTGATGAGGGTTTTGATTCTATGACTGATGATCGGGAGTGGGGTGCTCGCATGACAAAATCAAGCTTGGTTCCTCCTGTTACGAGGAAATATGAAGTTATTGAGGAATATGTTATTGTATCCAATGAGGAGGATGTGAAACGGAAGATGCAGGTATCTTTACCAGATGATTATGTTGAGAAACTTAATTCTCAGAAGAATGGAATTGAGGAGTCGGATATGGAGCTTCCTGAAGTAAAGGATTACAAGCCAAGGAAAATGCTTGGAGATGAAGTTATAGAGCAAGAAGTTTATGGAATTGATCCTTATAGCCACAATCTTTTACTTGACTCCATGCCAGAGGAGTTCGACTGGCCTCTTGCGGAGAAGCATATGTTTATCGAAGATGTGCTCCTTCGCACATTGAATAAGCAAGTCAGGCGGTACACTGGGAGTGGAAATACTCCCATGATGGTTCCCTTGCATCCTGTTGTTGAAGAAATCCTAAACGGTGCTGAGGAGGATGGTGATGTGCGAACTGTTAGGATGTGTCAGGCTATTTTGAAGGCCATAGAGAGCCGACATGATGATAAGTATGTGGCATATAGAAAGGGGCTTGGTGTCGTTTGCAACAAAGAAGGCGGCTTTGGAGAGGAAGATTTTGTTGTGGAATTTTTGGGGGAGGTTTATCCTGTTTGGAAATGGTTCGAGAAGCAAGACGGGATTCGATCTTTGCAGAAGAATAATAAAGATCCAGCTCCAGAGTTTTACAACATTTATCTTGAAAGACCCAAGGGTGATGCCGATGGGTATGATCTAGTCGTTGTGGATGCCATGCATAAGGCAAACTATGCAAGTAGAATTTGTCACTCATGCCGACCTAATTGCGAAGCAAAAGTTACTGCTGTAGATGGTCGTTACCAAATTGGAATCTACACTGTACAAAAAATTCAATATGGTGAGGAGGTGACATTCGATTACAATTCCGTTACAGAGAGTAAGGAAGAATACGAAGCATCAGTTTGTTTGTGTGGCAGCCAAGTTTGCCGGGGAAGCTACTTAAATTTGACAGGCGAAGGTGCTTTTCAAAAGGTGCTGAAAGAGTGGCATGGAGCTCTTGATCGTCATCAGTTAATGCTGGAGGCTTGTGAGTTAAATTCAGTATCTGAGGAGGACTATCTTGAGTTGGGAAGAGCTGGTTTAGGCAGTTGCTTGCTGGGTGGGTTGCCAGATTGGGTGATTGCATATTCAGCTCGCTTGGTGAGGTTTATAAATTTTGAAAGAACAAAGCTTCCTGAGGAGATTTTAAAGCACAATTTGGAAGAAAAGAGGAAGTACTTTTCAGATATTTTCCTTGAGGTTGAGAAAAGTGATGCTGAGGTTCAGGCTGAGGGCGTGTACAACCAAAGGCTTCAGAATTTGGCTGTAACTCTTGACAAGGTGAGGTATGTTATGAAATGCGCTTTTGGCAACCCCAAGGATGCTCCACCTCCGCTGGAAAGGTTAAGTCCTGAAGAGGCTGTTTCCTTTCTATGGAAGGGAGAAGGATCACTTGTACAGGAGCTTCTTCAGAGCATGGCTCCTCATGTGGAGGAAAACTTGCTAAATGATCTACGGATGAAGATCCTTGCTCGTGATCCATCAGGTTCGGATGACATATGGAAAGAACTTAAGAGATCTTTATTATGGTTGAGAGATGAAGTCCGGAATCTTCCTTGTACATACAAGTCTAGAAACGATGCTGCTGCTGACTTGATCCATATTTATGCTTACACACGGTGTTTTATTAGAATACGGGAATACAAAACTGTAACTTCGCCACCAGTATTCATTAGTCCACTTGACCTAGGCCCCAAGTACACTGAGACGCTGGGATCAGGTTTTCAGGAGTATTGCAAGATGTATGGGGAGAATTATTGTTTAGGGCAGCTGATTTTTTGGTATAGCCAGACTAGTGCAGAGCCGGATTGTAGCCTGGCAAGAGCAAGTAGGGGTTGCTTGTCGTTACCAGACTTCAGTTCCTTCTATGCCAAGGTTCAGAAACCTTCACGACAGCGTGTTTATGGCCCAAGAACAGTAAAGTTTATGCTAGCGAGGATGGAGAAGCAGCCGCAGAGACCTTGGCCTAAGGATCGAATATGGTCGTTCAGTAACTCTCCAAAAGTTATCGCCAGTCCTATGCTAGACGCTGTTGTGAATAAATCTCATTTGGACAGGGAGATGGTGCATTGGTTAAAGCACAGACCCGCGATATTTCAGGCTATGTGGGATCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009791 GO:0009908 GO:0009987 GO:0010228 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019538 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0034968 GO:0036211 GO:0042054 GO:0042800 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048443 GO:0048466 GO:0048467 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0051276 GO:0051568 GO:0061458 GO:0071704 GO:0071840 GO:0090567 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SET

Protein Analysis

2462

Amino Acids

280.32

Weight (kDa)

6.62

Isoelectric Point (pI)

55.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GYF_ATXR3	PF25531	656 - 703	3.4e-29	Histone-lysine N-methyltransferase ATXR3, GYF domain
GYF_ATXR3	PF25531	1062 - 1093	1.2e-07	Histone-lysine N-methyltransferase ATXR3, GYF domain
SET	PF00856	1749 - 2031	1e-11	SET domain
SDG2_C	PF19633	2042 - 2461	6.5e-240	Protein SET DOMAIN GROUP 2 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013012)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15180
fragaria_vesca	FvH4_6g43760
malus_domestica	MD09G1103200.v1.1 MD17G1091000.v1.1
prunus_persica	Prupe.3G222600_v2.0.a1
pyrus_communis	pycom09g02660 pycom17g08780
rosa_chinensis	RchiOBHm_Chr2g0160501
rosa_laevigata	RLG00000021218
rosa_multiflora	Rmu_sc0000674.1_g000001
rosa_roxburghii	Rroxscaffold_2G00089780
rosa_rugosa	Rorug02G0483400 Rorug02G0483400
rosa_samantha	Rh2AG548700 Rh2BG562400 Rh2CG533000 Rh2DG571100
rosa_wichuraiana	Rw2G045430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	3936, 4712, 6380
AasI	GACNNNNNNGTC	2 cut(s)	1167, 2069
Acc16I	TGCGCA	1 cut(s)	2229
Acc36I	ACCTGC	5 cut(s)	627, 2453, 3412, 3480, 5272
AccB7I	CCANNNNNTGG	2 cut(s)	2285, 2741
AccI	GTMKAC	2 cut(s)	1606, 3740
AccII	CGCG	4 cut(s)	1407, 1428, 1470, 7358
AccIII	TCCGGA	2 cut(s)	3294, 6813
AclI	AACGTT	1 cut(s)	4396
AcoI	YGGCCR	5 cut(s)	1297, 1360, 1381, 1402, 3555
AcyI	GRCGYC	1 cut(s)	15
AdeI	CACNNNGTG	1 cut(s)	6888
AflII	CTTAAG	1 cut(s)	6779
AflIII	ACRYGT	3 cut(s)	1936, 3003, 4736
AgeI	ACCGGT	1 cut(s)	1696
AhlI	ACTAGT	2 cut(s)	2173, 7061
AjiI	CACGTC	1 cut(s)	3006
AjuI	GAANNNNNNNTTGG	6 cut(s)	3164, 3196, 4892, 4924, 5739, 5771
AleI	CACNNNNGTG	1 cut(s)	3183
AloI	GAACNNNNNNTCC	8 cut(s)	188, 220, 477, 509, 2896, 2928, 6385, 6417
Alw21I	GWGCWC	4 cut(s)	3449, 5179, 5523, 6226
AlwNI	CAGNNNCTG	6 cut(s)	663, 2216, 3404, 4134, 4174, 6275
Aor13HI	TCCGGA	2 cut(s)	3294, 6813
ApaI	GGGCCC	1 cut(s)	1201
ArsI	GACNNNNNNTTYG	2 cut(s)	2573, 2605
AseI	ATTAAT	2 cut(s)	891, 3585
AsiGI	ACCGGT	1 cut(s)	1696
Asp700I	GAANNNNTTC	1 cut(s)	5813
AspA2I	CCTAGG	1 cut(s)	6955
AspLEI	GCGC	6 cut(s)	1236, 1428, 1659, 2230, 2291, 6572
AsuC2I	CCSGG	9 cut(s)	1217, 1385, 1943, 1966, 2517, 3542, 3667, 4379, 6158
AsuNHI	GCTAGC	2 cut(s)	239, 7198
AvaII	GGWCC	7 cut(s)	531, 1587, 2006, 3224, 3401, 3974, 4422
AvrII	CCTAGG	1 cut(s)	6955
AxyI	CCTNAGG	2 cut(s)	6405, 7236
BaeGI	GKGCMC	3 cut(s)	1201, 3216, 4566
BamHI	GGATCC	1 cut(s)	1708
BanII	GRGCYC	6 cut(s)	1201, 2053, 3110, 3449, 3667, 6226
BarI	GAAGNNNNNNTAC	6 cut(s)	676, 708, 3608, 3640, 6657, 6689
BauI	CACGAG	2 cut(s)	3846, 6741
Bbv12I	GWGCWC	4 cut(s)	3449, 5179, 5523, 6226
BbvCI	CCTCAGC	4 cut(s)	279, 5625, 6486, 6498
BceAI	ACGGC	3 cut(s)	1221, 1284, 1347
BcgI	CGANNNNNNTGC	8 cut(s)	496, 530, 3445, 3479, 5976, 6010, 6836, 6870
BciVI	GTATCC	1 cut(s)	5260
BclI	TGATCA	3 cut(s)	1210, 2497, 3259
BcnI	CCSGG	9 cut(s)	1217, 1385, 1943, 1966, 2517, 3542, 3667, 4379, 6158
BcuI	ACTAGT	2 cut(s)	2173, 7061
BfmI	CTRYAG	7 cut(s)	1884, 2460, 2547, 3245, 3333, 3775, 6015
BfrI	CTTAAG	1 cut(s)	6779
BfuAI	ACCTGC	5 cut(s)	627, 2453, 3412, 3480, 5272
BfuI	GTATCC	1 cut(s)	5260
BglII	AGATCT	5 cut(s)	1244, 1475, 2326, 3383, 6785
BlnI	CCTAGG	1 cut(s)	6955
BmcAI	AGTACT	4 cut(s)	1091, 4874, 5050, 6446
Bme18I	GGWCC	7 cut(s)	531, 1587, 2006, 3224, 3401, 3974, 4422
BmgBI	CACGTC	1 cut(s)	3006
BmrI	ACTGGG	5 cut(s)	230, 618, 3067, 5109, 5568
BmtI	GCTAGC	2 cut(s)	243, 7202
BmuI	ACTGGG	5 cut(s)	230, 618, 3067, 5109, 5568
BoxI	GACNNNNGTC	1 cut(s)	1629
BplI	GAGNNNNNCTC	4 cut(s)	1510, 1542, 6673, 6705
BpmI	CTGGAG	8 cut(s)	556, 1081, 2169, 2238, 3934, 4713, 5853, 6269
Bpu10I	CCTNAGC	5 cut(s)	279, 1017, 5625, 6486, 6498
BpuEI	CTTGAG	3 cut(s)	2862, 6311, 6485
BpuMI	CCSGG	9 cut(s)	1217, 1385, 1943, 1966, 2517, 3542, 3667, 4379, 6158
Bsa29I	ATCGAT	1 cut(s)	7381
BsaAI	YACGTR	2 cut(s)	3498, 3650
BsaBI	GATNNNNATC	2 cut(s)	6234, 6731
BsaHI	GRCGYC	1 cut(s)	15
BsaI	GGTCTC	2 cut(s)	532, 7219
BsaWI	WCCGGW	5 cut(s)	1515, 1696, 2914, 3294, 6813
BsaXI	ACNNNNNCTCC	8 cut(s)	387, 417, 477, 507, 1076, 1106, 5388, 5418
Bse118I	RCCGGY	1 cut(s)	1696
Bse21I	CCTNAGG	2 cut(s)	6405, 7236
Bse3DI	GCAATG	3 cut(s)	862, 1229, 4306
Bse8I	GATNNNNATC	2 cut(s)	6234, 6731
BseAI	TCCGGA	2 cut(s)	3294, 6813
BseCI	ATCGAT	1 cut(s)	7381
BseJI	GATNNNNATC	2 cut(s)	6234, 6731
BseMI	GCAATG	3 cut(s)	862, 1229, 4306
BseSI	GKGCMC	3 cut(s)	1201, 3216, 4566
BseYI	CCCAGC	4 cut(s)	3209, 3816, 6325, 6979
BsgI	GTGCAG	6 cut(s)	436, 1721, 2550, 3967, 4675, 7086
Bsh1236I	CGCG	4 cut(s)	1407, 1428, 1470, 7358
Bsh1285I	CGRYCG	1 cut(s)	1537
BshTI	ACCGGT	1 cut(s)	1696
BshVI	ATCGAT	1 cut(s)	7381
BsiEI	CGRYCG	1 cut(s)	1537
BsiHKAI	GWGCWC	4 cut(s)	3449, 5179, 5523, 6226
BsiWI	CGTACG	1 cut(s)	683
BslFI	GGGAC	5 cut(s)	328, 485, 1232, 1573, 1925
BsmBI	CGTCTC	2 cut(s)	1833, 6968
BsmFI	GGGAC	5 cut(s)	328, 485, 1232, 1573, 1925
Bso31I	GGTCTC	2 cut(s)	532, 7219
Bsp120I	GGGCCC	1 cut(s)	1197
Bsp13I	TCCGGA	2 cut(s)	3294, 6813
Bsp1407I	TGTACA	5 cut(s)	4389, 6049, 6507, 6664, 6827
Bsp19I	CCATGG	2 cut(s)	2950, 3578
Bsp68I	TCGCGA	1 cut(s)	1470
BspDI	ATCGAT	1 cut(s)	7381
BspEI	TCCGGA	2 cut(s)	3294, 6813
BspFNI	CGCG	4 cut(s)	1407, 1428, 1470, 7358
BspHI	TCATGA	3 cut(s)	1594, 3511, 4533
BspMAI	CTGCAG	4 cut(s)	1888, 2464, 2551, 3337
BspMI	ACCTGC	5 cut(s)	627, 2453, 3412, 3480, 5272
BspOI	GCTAGC	2 cut(s)	243, 7202
BspQI	GCTCTTC	2 cut(s)	3115, 6295
BspTI	CTTAAG	1 cut(s)	6779
BspTNI	GGTCTC	2 cut(s)	532, 7219
BsrDI	GCAATG	3 cut(s)	862, 1229, 4306
BsrFI	RCCGGY	1 cut(s)	1696
BsrGI	TGTACA	5 cut(s)	4389, 6049, 6507, 6664, 6827
BssAI	RCCGGY	1 cut(s)	1696
BssNI	GRCGYC	1 cut(s)	15
BssSI	CACGAG	2 cut(s)	3846, 6741
Bst2BI	CACGAG	2 cut(s)	3846, 6741
BstACI	GRCGYC	1 cut(s)	15
BstAFI	CTTAAG	1 cut(s)	6779
BstAPI	GCANNNNNTGC	3 cut(s)	3472, 3653, 5960
BstAUI	TGTACA	5 cut(s)	4389, 6049, 6507, 6664, 6827
BstBAI	YACGTR	2 cut(s)	3498, 3650
BstDSI	CCRYGG	3 cut(s)	2950, 2986, 3578
BstEII	GGTNACC	1 cut(s)	2128
BstFNI	CGCG	4 cut(s)	1407, 1428, 1470, 7358
BstHHI	GCGC	6 cut(s)	1236, 1428, 1659, 2230, 2291, 6572
BstMCI	CGRYCG	1 cut(s)	1537
BstNSI	RCATGY	4 cut(s)	24, 28, 1940, 2937
BstPAI	GACNNNNGTC	1 cut(s)	1629
BstPI	GGTNACC	1 cut(s)	2128
BstSFI	CTRYAG	7 cut(s)	1884, 2460, 2547, 3245, 3333, 3775, 6015
BstSLI	GKGCMC	3 cut(s)	1201, 3216, 4566
BstUI	CGCG	4 cut(s)	1407, 1428, 1470, 7358
BstXI	CCANNNNNNTGG	1 cut(s)	585
Bsu15I	ATCGAT	1 cut(s)	7381
Bsu36I	CCTNAGG	2 cut(s)	6405, 7236
BsuI	GTATCC	1 cut(s)	5260
BsuTUI	ATCGAT	1 cut(s)	7381
BtgI	CCRYGG	3 cut(s)	2950, 2986, 3578
BtgZI	GCGATG	1 cut(s)	21
BtrI	CACGTC	1 cut(s)	3006
BtsI	GCAGTG	2 cut(s)	250, 2457
BtuMI	TCGCGA	1 cut(s)	1470
BveI	ACCTGC	5 cut(s)	627, 2453, 3412, 3480, 5272
CaiI	CAGNNNCTG	6 cut(s)	663, 2216, 3404, 4134, 4174, 6275
CciI	TCATGA	3 cut(s)	1594, 3511, 4533
CfoI	GCGC	6 cut(s)	1236, 1428, 1659, 2230, 2291, 6572
Cfr10I	RCCGGY	1 cut(s)	1696
ClaI	ATCGAT	1 cut(s)	7381
CseI	GACGC	4 cut(s)	4, 2591, 6985, 7304
CspAI	ACCGGT	1 cut(s)	1696
CspCI	CAANNNNNGTGG	2 cut(s)	180, 215
DraI	TTTAAA	1 cut(s)	6417
DraIII	CACNNNGTG	1 cut(s)	6888
DrdI	GACNNNNNNGTC	2 cut(s)	1167, 2069
DseDI	GACNNNNNNGTC	2 cut(s)	1167, 2069
EaeI	YGGCCR	5 cut(s)	1297, 1360, 1381, 1402, 3555
EciI	GGCGGA	1 cut(s)	1951
Ecl136II	GAGCTC	2 cut(s)	3447, 6224
Eco147I	AGGCCT	1 cut(s)	1102
Eco24I	GRGCYC	6 cut(s)	1201, 2053, 3110, 3449, 3667, 6226
Eco31I	GGTCTC	2 cut(s)	532, 7219
Eco32I	GATATC	3 cut(s)	4126, 4531, 4642
Eco47I	GGWCC	7 cut(s)	531, 1587, 2006, 3224, 3401, 3974, 4422
Eco53kI	GAGCTC	2 cut(s)	3447, 6224
Eco81I	CCTNAGG	2 cut(s)	6405, 7236
Eco91I	GGTNACC	1 cut(s)	2128
EcoICRI	GAGCTC	2 cut(s)	3447, 6224
EcoO109I	RGGNCCY	5 cut(s)	531, 3224, 3401, 3974, 6959
EcoO65I	GGTNACC	1 cut(s)	2128
EcoRI	GAATTC	2 cut(s)	524, 3877
EcoRV	GATATC	3 cut(s)	4126, 4531, 4642
EcoT22I	ATGCAT	5 cut(s)	26, 2935, 3122, 4930, 5949
EcoT38I	GRGCYC	6 cut(s)	1201, 2053, 3110, 3449, 3667, 6226
Esp3I	CGTCTC	2 cut(s)	1833, 6968
FalI	AAGNNNNNCTT	6 cut(s)	2037, 2069, 4077, 4109, 6645, 6677
FaqI	GGGAC	5 cut(s)	328, 485, 1232, 1573, 1925
FauI	CCCGC	2 cut(s)	4082, 7363
FauNDI	CATATG	8 cut(s)	1252, 1273, 1399, 1441, 1483, 3357, 5502, 6767
FbaI	TGATCA	3 cut(s)	1210, 2497, 3259
FblI	GTMKAC	2 cut(s)	1606, 3740
FriOI	GRGCYC	6 cut(s)	1201, 2053, 3110, 3449, 3667, 6226
FspI	TGCGCA	1 cut(s)	2229
GlaI	GCGC	6 cut(s)	1235, 1427, 1658, 2229, 2290, 6571
GsaI	CCCAGC	4 cut(s)	3213, 3820, 6329, 6983
GsuI	CTGGAG	8 cut(s)	556, 1081, 2169, 2238, 3934, 4713, 5853, 6269
HgaI	GACGC	4 cut(s)	4, 2591, 6985, 7304
HhaI	GCGC	6 cut(s)	1236, 1428, 1659, 2230, 2291, 6572
Hin1I	GRCGYC	1 cut(s)	15
Hin6I	GCGC	6 cut(s)	1234, 1426, 1657, 2228, 2289, 6570
HinP1I	GCGC	6 cut(s)	1234, 1426, 1657, 2228, 2289, 6570
HindIII	AAGCTT	4 cut(s)	2667, 3506, 5194, 6398
Hpy99I	CGWCG	1 cut(s)	3099
HpyCH4IV	ACGT	8 cut(s)	198, 2002, 3005, 3125, 3497, 3649, 4396, 4738
HpySE526I	ACGT	8 cut(s)	198, 2002, 3005, 3125, 3497, 3649, 4396, 4738
Hsp92I	GRCGYC	1 cut(s)	15
HspAI	GCGC	6 cut(s)	1234, 1426, 1657, 2228, 2289, 6570
Kpn2I	TCCGGA	2 cut(s)	3294, 6813
Ksp22I	TGATCA	3 cut(s)	1210, 2497, 3259
LguI	GCTCTTC	2 cut(s)	3115, 6295
MaeII	ACGT	8 cut(s)	198, 2002, 3005, 3125, 3497, 3649, 4396, 4738
MmeI	TCCRAC	2 cut(s)	1824, 5325
Mph1103I	ATGCAT	5 cut(s)	26, 2935, 3122, 4930, 5949
MroI	TCCGGA	2 cut(s)	3294, 6813
MroXI	GAANNNNTTC	1 cut(s)	5813
MslI	CAYNNNNRTG	7 cut(s)	23, 1299, 1362, 1869, 3183, 3285, 6873
MspA1I	CMGCKG	2 cut(s)	6604, 7039
MspCI	CTTAAG	1 cut(s)	6779
MvnI	CGCG	4 cut(s)	1407, 1428, 1470, 7358
NciI	CCSGG	9 cut(s)	1217, 1385, 1943, 1966, 2517, 3542, 3667, 4379, 6158
NcoI	CCATGG	2 cut(s)	2950, 3578
NdeI	CATATG	8 cut(s)	1252, 1273, 1399, 1441, 1483, 3357, 5502, 6767
NheI	GCTAGC	2 cut(s)	239, 7198
NmeAIII	GCCGAG	1 cut(s)	4640
NruI	TCGCGA	1 cut(s)	1470
NsbI	TGCGCA	1 cut(s)	2229
NsiI	ATGCAT	5 cut(s)	26, 2935, 3122, 4930, 5949
NspI	RCATGY	4 cut(s)	24, 28, 1940, 2937
OliI	CACNNNNGTG	1 cut(s)	3183
PaeI	GCATGC	2 cut(s)	28, 2937
PagI	TCATGA	3 cut(s)	1594, 3511, 4533
PceI	AGGCCT	1 cut(s)	1102
PciI	ACATGT	1 cut(s)	1936
PciSI	GCTCTTC	2 cut(s)	3115, 6295
PcsI	WCGNNNNNNNCGW	1 cut(s)	204
PdmI	GAANNNNTTC	1 cut(s)	5813
Pfl23II	CGTACG	1 cut(s)	683
PflMI	CCANNNNNTGG	2 cut(s)	2285, 2741
PfoI	TCCNGGA	2 cut(s)	533, 1156
PinAI	ACCGGT	1 cut(s)	1696
Ple19I	CGATCG	1 cut(s)	1537
Ppu21I	YACGTR	2 cut(s)	3498, 3650
PpuMI	RGGWCCY	4 cut(s)	531, 3224, 3401, 3974
PscI	ACATGT	1 cut(s)	1936
PshAI	GACNNNNGTC	1 cut(s)	1629
PshBI	ATTAAT	2 cut(s)	891, 3585
PsiI	TTATAA	3 cut(s)	3936, 4712, 6380
Psp124BI	GAGCTC	2 cut(s)	3449, 6226
Psp1406I	AACGTT	1 cut(s)	4396
Psp5II	RGGWCCY	4 cut(s)	531, 3224, 3401, 3974
PspEI	GGTNACC	1 cut(s)	2128
PspFI	CCCAGC	4 cut(s)	3209, 3816, 6325, 6979
PspLI	CGTACG	1 cut(s)	683
PspOMI	GGGCCC	1 cut(s)	1197
PspPPI	RGGWCCY	4 cut(s)	531, 3224, 3401, 3974
PstI	CTGCAG	4 cut(s)	1888, 2464, 2551, 3337
PstNI	CAGNNNCTG	6 cut(s)	663, 2216, 3404, 4134, 4174, 6275
PvuI	CGATCG	1 cut(s)	1537
PvuII	CAGCTG	1 cut(s)	7039
RruI	TCGCGA	1 cut(s)	1470
RseI	CAYNNNNRTG	7 cut(s)	23, 1299, 1362, 1869, 3183, 3285, 6873
SacI	GAGCTC	2 cut(s)	3449, 6226
SapI	GCTCTTC	2 cut(s)	3115, 6295
ScaI	AGTACT	4 cut(s)	1091, 4874, 5050, 6446
SfcI	CTRYAG	7 cut(s)	1884, 2460, 2547, 3245, 3333, 3775, 6015
SinI	GGWCC	7 cut(s)	531, 1587, 2006, 3224, 3401, 3974, 4422
SmiMI	CAYNNNNRTG	7 cut(s)	23, 1299, 1362, 1869, 3183, 3285, 6873
SmlI	CTYRAG	4 cut(s)	2877, 6290, 6464, 6779
SmoI	CTYRAG	4 cut(s)	2877, 6290, 6464, 6779
SpeI	ACTAGT	2 cut(s)	2173, 7061
SphI	GCATGC	2 cut(s)	28, 2937
SseBI	AGGCCT	1 cut(s)	1102
SstI	GAGCTC	2 cut(s)	3449, 6226
StuI	AGGCCT	1 cut(s)	1102
TaiI	ACGT	8 cut(s)	201, 2005, 3008, 3128, 3500, 3652, 4399, 4741
TaqII	GACCGA	1 cut(s)	3009
TauI	GCSGC	5 cut(s)	1407, 2278, 3557, 5754, 7222
TspGWI	ACGGA	3 cut(s)	5287, 6085, 6739
Van91I	CCANNNNNTGG	2 cut(s)	2285, 2741
Vha464I	CTTAAG	1 cut(s)	6779
VpaK11BI	GGWCC	7 cut(s)	531, 1587, 2006, 3224, 3401, 3974, 4422
VspI	ATTAAT	2 cut(s)	891, 3585
XbaI	TCTAGA	4 cut(s)	1750, 3571, 4280, 6839
XceI	RCATGY	4 cut(s)	24, 28, 1940, 2937
XmaJI	CCTAGG	1 cut(s)	6955
XmiI	GTMKAC	2 cut(s)	1606, 3740
XmnI	GAANNNNTTC	1 cut(s)	5813
ZrmI	AGTACT	4 cut(s)	1091, 4874, 5050, 6446
Zsp2I	ATGCAT	5 cut(s)	26, 2935, 3122, 4930, 5949

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.