Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0013012

Physical Location & Seq

Genomic Coordinates Reverse (-)

77335603 .. 77347941

Gene Structure 12339 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG562400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7413 bp

ATGGGCGATGGAGGAGTCGCATGCATGCCTTTGCAGCATAATATCATGGACAGGTTTCCAATTCCGGAGAAGACTGCGCTTTGCGGAGGCAATACTAATACTAATGCTACTACTACTACTACCACCACCGCTAACAATGGCTTCAATTCCAAGCCGGTTGTTAAGAAGAAGGTAATGAAGATCAAGAAGAGGATTGTCAGGAAGCCCAAGGCAAAAAGCAACAGTAACGTAGAGTCGGGGAAGAGTGAGCTGGGATTGGTTAAAGGTGAGAAGAGTGATACTACTAAGGAAGCAGAGAACAGTGGTGGTGCCAAGGAAGCAGCCGAGAAGGAAGCTGAGAACAGTGCTGCTCAGCAAGTTGAGAACGTCGAAAATAATGGTGAGGATAAGAGGGAGGAAGTGGAAGAGGGTGAATTGGGGACTTTGAATGGTGAATTTGTTCCGGACAAGTGGCGGAGAAGCGAGGTTGAGAAGGAGGAGATTGGGGGTGAGAAATGGAGGAGAAGCGATGTGGAGAAGGGAGAGTCTTTTTCTGGGAAATGGCGTAGAGGTGATGCAGAGAAGGCAGATATATATCCCGAGAAGAGTAGAAATAGTGAAGCTGAATTCGGCTCGTGGAGACCACCTAAGGATGAAATTGAAAGAGGTGAGTATATTCCAGATAGATGGCAAAAAGGAGAGCTGGCCAGAGATGACTACAGTAACCGAAAAATGCCCGCCAGGTACGATATGGGTGGGAGGGGCAGGGGAAGGGGCTGGAAATTTGAGTCCCCTTCTGGCAAGTATCCAAATGATGACGGGTTTAGAAGGAAAGAATATAGTAGAGGTGGGGGTCAGCACAGCAAGAGTACTTTCAGGTGGGATAGTGGCCAGGAGAGGAATACGAGGATCAGTTCCAAAATTGTTGATGATGAGGGTGTCTACAAGAATGAGTACAGCAGTGAGTACATCAATGGTAAGTATCAAGCAAAGGATTACTCTTCTGTTAATCGGTTGAAGAGGTATGGTCCTGATTCAAATAGTAGTGAGCGGAAGCACTATGGAGATTATGGGGATTATGCGGGTTTAAAAAGTCGCAAGCTTTCTGATGAAAGTAATCGCTCTGCTCACTCTGAGCATTATTCACACCGTTCTGGGGAGAGATCTTATCGAAATCCTTCTTTTAGACCAGCATCAGACAAGTACTCTTCCAGGCACTATGAACCTACTTTGTCTTCCAGAGTGGTTTATGACAGGCATGGGCGAAGTCCAGGTCTCTCTGAGCGGTCCCCACGTGACAGGTCCCGGTATTATGATCACCTGGACCGGAGTCCAGTGCGCCGTGAGAGATCTCCATATGATCTAGAGAGGTCCCCTTATGGCCGTGAGAAATCACCATATGGCCGTGAGAGATCTCCATATGGTCATGAGAAATCTCCGTATGTACGTGATAAATCTCCACATGTTCGTGACAGATCTCCATATGTTCGCGACAAGTCTCCATATGCTCGCGAGAAGTCACCATATGGACGTGAAAGATCTCCATATGTTCGTGACAGATCTCCTTATGTTCTGGAGAGATCTCCATACGAACGGAGCCGTAATTATGATCACAGAAACCGCAGTTTGTCTCCACAAGATCGACCTAGGTACCATGATCGCAGGGATCACACCCCAAACTATTTGGAGCGGTCACCACGTGGTGACCGGGGTAGAGCTTCTAGTTATAGAGGTACAGGCCGCAAAAGTGGAGCAAGTGATAGGCGCAACTCCCAATATAAAGCGCACGAAGATAAGCTGGTGCAGAAGGATCCCACGGGAACGGACTCAAATTCCTCTGCCAAAGAATGTCAGGATAGAAGCAGTGTTCTTGACATTAATGGAACTGTGGAGACAAACACCAATTCTGAGTCTCACAAAGAAGAACCGTCTCAAAGTCCAAATATAAATTGCAAAGAAACTTCTCATATTAGTGCAGCCCCTCCTGAAGAGCTGCCTTCTATGGAAGAAGATATGGATATATGCGACACACCACCACATGTCCCAGTGATTGCTGATTCATCCACAGGGAAATGGTTTTACCTTGATTATTATGGTGTGGAATGTGGGCCTTCCAAATTATGTGAGCTTAAAGCACTTGTTGAAGAAGGAGCTCTGATGTCCGATCACATGGTCAAGCACTCAGACAGTGATAGGTGGGTAACTGTTGAAAATGCAGTTTCGCCATTGATTACCGTAAATTTTCCATCCATTGTATCGGACTCGATAACTGGACTAGTAAGCCCTCCAGAAGCTCCTGGCAATCTATTGGCAGATATTGGACATACTGGGCACTATGGTACTCAGAGTGGGAGTTTACCGGGGTTGTGTGCAGATGTTGCTTCTGAGCCTCTAGAAGATTTGCACATTGAAGAAAGGGTTGGGGCTCTGATGGAAGGTTTAACAGTTATTCCTGGCAGGGAACTTGAAGCTGTTGGAGAAGTTCTGCAAATGTCATTTGAATCTGCTCAACTGGAAGTATGGGGAAACAGTGAAGGTCTCTCTCAAGGTCATATCGGTGAACAGAATGATCAGAAAACTGAGGAACCACGATTTTCTGACATTAAAATGAAAGAAGCAGCAGAAATAGGGTTGACTTTACCCTCTGACAAAGATGCTTTTGCTTGTGGTGATTCCGGTGACTGGTTTTCTGGTCGATGGTCATGCAAGGGTGGTGATTGGAAAAGGAACGAAGAAGGTGTGCAAGATAGGTCTTCTAGAAAGAAACTTGTTGTCAATGATGGTTTCCCATTATGCCAGATGTCAAAATCTGGGTATGAAGACCCTCGATGGCAAAGAAAAGATGAACTGTATTATCCTTCACAGAACAGAAGGCTTGATCTACCTACTTGGGCTTTTTCATGTCCAGATGATGCTAATAGAGTGTCTCAAAGTAAGCCAACTGTTATAAAAGGAGTAAAAGGAACTATCCTTCCGGTAGTCAAGATAAATGCATGTGTTGTAAAAGACCACGGTTCATTTGTTTCTGAGCCGCGGATAAAAGCACGAGGAATGGAAAGGCATCCTTCAAGGTCTGCTCGGTCATATTCCGCAAGTAGTGATGGCAAGAGATCATCTGGCGATGGTGATTCTCAAATGAAACCAGTAGGTGACCGAGGCTCAAAGGGATCTTCCAAATGCGTTTCCTCCATTAAAATCCCCAAAGACCGTATTGGCACAGTTGATGACTTGCAATTGCATTTGGGAGACTGGTACTACCTTGATGGTGCTGGGCATGAACGAGGACCTTCATCATTCTCTGAGCTACAGGCCTTAGTTGATCAAGGAGTGATCCTGAAACATAGCAGTGTGTTCCGGAAATTTGATAAAGTCTGGGTTCCAGTTACCTCTGCCATTGAGACTTCTGATGCTACCAAAATGAATAAGGAGAAGAACAGAACCTCAAGTAATTTTTCAGGGCAATCTCAAAGTGCTACACTTGCAGAATCAAAAACAAATTTGAGTTGGTTGCAGAATTTGCACCCTCAGTTCATTGGTTATACATGTGGAAAGCTTCATGAATTGGTGATGAAATCTTATAAAAGTCGGGAGTTTGCTGCAGCCATAAATGAGGTTTTAGATCCATGGATTAATGCAAGGCAGCCAAAAAAAGAGTTGGACAAGCACATGTACTGGAAAGCAGATGGTGATGCACGTTCTTCTAAAAGAACTCGGTTGCTGGTTGATGAAAGTGAAGAAGACTATGACGCAGAAGAGGACTTGCAAACAATAGAAAAGGATGATTCCGCATTTGAGGATCTAATTGGTAATGCTTCTTTCTTCAGAGAAGACAGCTTGAGTTATGGATCAGAGATGGCAAGCTGGGGTTTATTGGATGGACATGTGCTGGCACGGGTCTTTCATTTTTTAAGATTGGACATGAAATCTCTTACCATTGCTTCTCTGACTTGTAAGCACTGGAGAGCTGCGGTCAGTTTTTACAAGGATATTTCAAGACAGGTAGACTTGTCATCCTTGGGTCCCAACTGCACCGACTCCATGATTGTGAAGATTATGAGTGGTTATGGCAAAGAGAAGATTAATTCTATGGTTCTAATTGGTTGCACAAACATCACTCCCCACACTCTTGAAGAGATTCTTGGTTCACTTCCTTGTCTATCTACCATAGATATTAGGGGTTGCAACCAGTTTGGTGAGTTGGTCATTAAATTTCAGAACCTGAACTGGATTAAGAGCCGAAGCTCACGTGGTACAAAGATACATGATGAATCCAATTCAAAATTAAGGAGTCTGAAATATATCAGTGAGAAATCTTCTTATGTTTCGAGAAGTAAGGTTCTGGGTAATGATATGGATGATTTTAGTGAACTCAAAGTGTACTTTGATAGTGTGGATAAGAGAGACTCAGCTAATCAATCATTCCGGGGAAGTTTATACAAACGTTCAAAACTATTTGATGCTAGACGGTCCTCATCCATTCTATCTAGGGATGCTCGTATGAGGCGGTTGTCCATTAAGAAATCAGAAAATGGTTATAAGAGGATGGAGGAATTTGTTGCTTCAAGTCTGAAGGACATCATGAAGGAGAATACATCTGATTTCTTTGTACCCAAGGTTGCTGAAATTCAAGATAGAATGAGAAATGGGTATTACATTCGCCGAGGATTGAGTTCTGTGAAGGAGGATATCAGTCGAATGTGCAGGGATGCAATTAAAGCAAAGAATCGTGGTGAAGCTGGAGATATTAATCACCTGAATCACATTATTAATTTGTTTATACAACTTGCCACACGTTTGGAAGGGGCTTCCAAGTCTTCTGATGCAAGGGATGAGCTGATCAAGTCGTGGGAAGATGATACATTTGCAGGGGTTTCTTCTGCCTCCAAGAGTAAAAAGAAGCTCAATAAGACGTCTGAAAGGAAGTTGAGGAGTAATGGTTCCTTTATGAATGGTAGTGTGGATAATGGAGAATATGCATCTGATCGAGAAATCAGAAGGCGTTTATCCAAGTTGAATAAAAAATCTATGGATTCTGAAAGTGAAACATCTGATGATCTTGATAGGTCATCTGAAGATAGCAAGAGCAATACTGAGAGTACTGCCTCGGATACAGAAAGTGACTTGGAATCTGGGTCACAGAGTCAACCTGGGCAATCAACAGCAGATGGATGCTTAGCTCATGATGAGGGTTCAGATTCTGTGACTGATGATCGTGAGTGGGGAGCTCGCATGACCAAATCAAGCTTGGTTCCTCCTGTTACGAGAAAATATGAAGTCATTCATGAATATGTTATTGTATCGCATGAAGAGGATGTAAAGCGGAAGATGCAGGTATCTTTGCCGGATGACTATGTTGAGAAGCTTTATTCGCAGAAGAATGGAACTGAGGAGTCTGATATGGAACTTCCTGAAGTCAAGGACTACAAGCCCAGGAAAATGCTTGGAGATGAGGTTCTAGAGCAAGAGGTTTATGGAATTGATCCCTACAGCCATAATCTTTTACTTGATTCCATGCCAGAGGAGTTGGATTGGCCTCTTTTAGAGAAGCATCTGTTTATAGAAGATGTGCTTCTTCGCACCCTGAATAAGGAAGTTAGACACTTTACTGGTACCGGAACTACTCCCATGATCTATCCTTTGCGGCCCGTTGTTGAAGATATTCGGAAAACTGCTGAAGAGGATAGTGATATTCGAACTGTGAGAATGTGCCAGGGTATTTTGAAGGCCATAGACAGTCGCCCTGATGATAAATATGTTGCTTATAGAAAGGGGCTCGGCGTTGTTTGCAACAAAGAAGAAGGTTTTGGAGAAGAAGATTTTGTTGTGGAATTTTTGGGGGAGGTATATCCTGTTTGGAAATGGTATGAGAAGCAAGATGGGATTCGTTCTCTGCAGAAAAACAGTAAAGATCCAGCTCCGGAATTTTACAACATTTATCTTGAAAGGCCCAAGGGTGATGCTGATGGGTATGATTTAGTTGTTGTTGATGCCATGCATAAAGCAAACTATGCAAGTAGAATTTGTCACTCGTGCCGACCTAATTGTGAAGCAAAAGTTACTGCTGTCGATGGTCGTTACCAGATCGGTATCTACACTGTACGTAAAATTCAATATGGTGAAGAGATCACATTTGACTACAACTCTGTTACGGAGAGTAAGGAAGAATATGAAGCATCAGTTTGTTTGTGTGGCAGTCAAGTTTGCCGGGGAAGTTACCTGAATTTGACAGGCGAAGGAGCATTCCTGAAGGTGCTGAAGGATTGGCATGGAATTCTGGATCGTCATCATCTAATGCTGGAAGCTTGCGAGTTAAATTCAGTATCTGAGGAGGATTATCTTGACCTGGGAAGAGCTGGTTTAGGCAATTGTTTGCTTGGTGGCTTGCCAGATTGGGTGATTGCATATTCAGCTCGTTTGGTGAGGTTCATAAACTTCGAAAGAACAAAGCTTCCTGAGGTGATTTTAAAGCACAACTTAGAAGAAAAGAGGAAGTATTTCTCAGATATATGTCTTGAGGTTGAGAAGAGTGACGCGGAGGTTCAGGCTGAGGGTGTGTTCAACCAAAGGCTTCAGAATTTGGCTGTTACTCTTGACAAGGTGAGGTATGTTATGAGCTCCGTATTTGGCAACCCAAAGAATGCTCCACCTCCACTGGAGAGGTTAAGTCCAGAAGAAGCTGTTGGCTTTGTATGGAAGGGAGAAGGATCAGTTGTTGAGGAACTCCTTCAGAGCATGGCTCCTCATGTGGAGGAACAATTGCTAAATGATCTCAAGTCCAAGATGCTTGCTCATGATCCATCGAGTTCAGATGACATTTGGAAAGAACTTAAAAGATCGTTACTATGGTTGAGAGATGAGGTCCGGAATCTTCCATGTACATACAAGTCTCGGCATGACGCTGCTGCTGACTTGATCCATATTTATGCATACACAAGGTGCTACATTAGAATACGGGAATACAAATCAGTAACTTCACCGCCGGTTTACATTAGTCCACTTGACTTGGGCCCCAACTACACTAACAAATCGGGGGCAGATTTTCAGGAGTATTGCAAGACTTACGGGGAGAATTATTGTTTAGGGCAGCTATTTTTTTGGTATAATCAGTCTAATGCAGAGCCAGATTGTAGTATGGCTAGAGCAAGTAGGGGTTGCTTATCATTACCTGATATCGGTTCCTTCTATGCCAAGATTTCGAAACCTACACGGCAGCGTGTTTATGGTCCAAAGGCTGTCAAATTTATGCTTTCAAGGATGGAGAAGCAGCCCCAAAAGCCTTGGCCCAAGGATCGAATATGGTCATTTAATAGCTCCCCAAAAGTTCTTGGCAGTCCAATGCTAGATGCTGTTGTGAATAACTCTTTCTTGGACAAAGAAATGTTGCATTGGTTAAAGCACAGACCTGCAATATATCAAGCTATGTGGGATCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009791 GO:0009908 GO:0009987 GO:0010228 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019538 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0034968 GO:0036211 GO:0042054 GO:0042800 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048443 GO:0048466 GO:0048467 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0051276 GO:0051568 GO:0061458 GO:0071704 GO:0071840 GO:0090567 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SET

Protein Analysis

2470

Amino Acids

280.04

Weight (kDa)

6.51

Isoelectric Point (pI)

54.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GYF_ATXR3	PF25531	683 - 730	2.1e-29	Histone-lysine N-methyltransferase ATXR3, GYF domain
GYF_ATXR3	PF25531	1075 - 1106	7.9e-08	Histone-lysine N-methyltransferase ATXR3, GYF domain
SET	PF00856	1832 - 2040	1.2e-12	SET domain
SDG2_C	PF19633	2051 - 2470	7.8e-237	Protein SET DOMAIN GROUP 2 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013012)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15180
fragaria_vesca	FvH4_6g43760
malus_domestica	MD09G1103200.v1.1 MD17G1091000.v1.1
prunus_persica	Prupe.3G222600_v2.0.a1
pyrus_communis	pycom09g02660 pycom17g08780
rosa_chinensis	RchiOBHm_Chr2g0160501
rosa_laevigata	RLG00000021218
rosa_multiflora	Rmu_sc0000674.1_g000001
rosa_roxburghii	Rroxscaffold_2G00089780
rosa_rugosa	Rorug02G0483400 Rorug02G0483400
rosa_samantha	Rh2AG548700 Rh2BG562400 Rh2CG533000 Rh2DG571100
rosa_wichuraiana	Rw2G045430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	2927, 3558, 4515
AatII	GACGTC	1 cut(s)	4892
Acc36I	ACCTGC	2 cut(s)	5299, 7390
Acc65I	GGTACC	2 cut(s)	1629, 5588
AccB1I	GGYRCC	3 cut(s)	308, 1629, 5588
AccBSI	CCGCTC	3 cut(s)	1030, 1264, 1669
AccI	GTMKAC	2 cut(s)	921, 3981
AccII	CGCG	4 cut(s)	1470, 1491, 3013, 6512
AccIII	TCCGGA	5 cut(s)	64, 442, 3333, 5896, 6840
AclI	AACGTT	1 cut(s)	4420
AcoI	YGGCCR	4 cut(s)	684, 868, 1360, 1381
AcvI	CACGTG	3 cut(s)	1274, 1679, 4226
AcyI	GRCGYC	1 cut(s)	4889
AdeI	CACNNNGTG	3 cut(s)	1679, 1682, 6915
AfiI	CCNNNNNNNGG	4 cut(s)	1135, 2661, 5566, 7151
AflIII	ACRYGT	6 cut(s)	1441, 2017, 3521, 3645, 3859, 4769
AhlI	ACTAGT	1 cut(s)	2254
AjiI	CACGTC	1 cut(s)	1511
AjuI	GAANNNNNNNTTGG	8 cut(s)	2382, 2414, 4101, 4133, 5766, 5798, 6642, 6674
AloI	GAACNNNNNNTCC	8 cut(s)	2935, 2967, 4900, 4932, 5415, 5447, 6412, 6444
Alw21I	GWGCWC	3 cut(s)	2134, 5206, 6596
AlwNI	CAGNNNCTG	3 cut(s)	4198, 5177, 6302
Ama87I	CYCGRG	1 cut(s)	578
Aor13HI	TCCGGA	5 cut(s)	64, 442, 3333, 5896, 6840
ApaI	GGGCCC	1 cut(s)	6989
ArsI	GACNNNNNNTTYG	2 cut(s)	1237, 1269
AseI	ATTAAT	5 cut(s)	1857, 3609, 4059, 4725, 4746
Asp700I	GAANNNNTTC	7 cut(s)	438, 1156, 4113, 5256, 5637, 6473, 6702
Asp718I	GGTACC	2 cut(s)	1629, 5588
AspA2I	CCTAGG	1 cut(s)	1625
AspLEI	GCGC	4 cut(s)	79, 1320, 1746, 1765
AsuC2I	CCSGG	5 cut(s)	1285, 1688, 2340, 4403, 6185
AsuII	TTCGAA	3 cut(s)	5671, 6414, 7175
AvaI	CYCGRG	1 cut(s)	578
AvrII	CCTAGG	1 cut(s)	1625
AxyI	CCTNAGG	2 cut(s)	627, 6432
BaeGI	GKGCMC	2 cut(s)	2313, 6989
BaeI	ACNNNNGTAYC	2 cut(s)	1613, 1646
BalI	TGGCCA	2 cut(s)	686, 870
BamHI	GGATCC	1 cut(s)	1789
BanI	GGYRCC	3 cut(s)	308, 1629, 5588
BanII	GRGCYC	6 cut(s)	2134, 2407, 5206, 5754, 6596, 6989
BauI	CACGAG	3 cut(s)	613, 3024, 6007
BbrPI	CACGTG	3 cut(s)	1274, 1679, 4226
BbsI	GAAGAC	7 cut(s)	77, 1206, 2724, 2805, 3723, 3813, 4785
Bbv12I	GWGCWC	3 cut(s)	2134, 5206, 6596
BbvCI	CCTCAGC	1 cut(s)	6525
BceAI	ACGGC	5 cut(s)	1305, 1347, 1368, 1563, 7202
BcgI	CGANNNNNNTGC	2 cut(s)	2664, 2698
BciVI	GTATCC	2 cut(s)	795, 5080
BclI	TGATCA	5 cut(s)	1294, 1588, 2548, 3298, 4815
BcnI	CCSGG	5 cut(s)	1285, 1688, 2340, 4403, 6185
BcuI	ACTAGT	1 cut(s)	2254
BfmI	CTRYAG	5 cut(s)	697, 3284, 3576, 5464, 5870
BfuAI	ACCTGC	2 cut(s)	5299, 7390
BfuI	GTATCC	2 cut(s)	795, 5080
BglII	AGATCT	7 cut(s)	1142, 1328, 1391, 1454, 1517, 1538, 1559
BlnI	CCTAGG	1 cut(s)	1625
BlpI	GCTNAGC	2 cut(s)	351, 5152
BmcAI	AGTACT	3 cut(s)	850, 1184, 5077
BmeT110I	CYCGRG	1 cut(s)	578
BmgBI	CACGTC	1 cut(s)	1511
BmrI	ACTGGG	2 cut(s)	2018, 2316
BmuI	ACTGGG	2 cut(s)	2018, 2316
BoxI	GACNNNNGTC	1 cut(s)	1308
BpiI	GAAGAC	7 cut(s)	77, 1206, 2724, 2805, 3723, 3813, 4785
BplI	GAGNNNNNCTC	2 cut(s)	6700, 6732
BpmI	CTGGAG	5 cut(s)	1574, 2250, 3958, 4737, 6653
Bpu10I	CCTNAGC	1 cut(s)	6525
Bpu1102I	GCTNAGC	2 cut(s)	351, 5152
Bpu14I	TTCGAA	3 cut(s)	5671, 6414, 7175
BpuEI	CTTGAG	5 cut(s)	2508, 3406, 3835, 6512, 6734
BpuMI	CCSGG	5 cut(s)	1285, 1688, 2340, 4403, 6185
BsaAI	YACGTR	5 cut(s)	1274, 1427, 1679, 4226, 6080
BsaBI	GATNNNNATC	4 cut(s)	573, 4725, 6065, 6261
BsaHI	GRCGYC	1 cut(s)	4889
BsaI	GGTCTC	3 cut(s)	613, 1259, 2522
BsaWI	WCCGGW	9 cut(s)	64, 442, 1305, 2654, 2953, 3333, 5591, 5896, 6840
BsaXI	ACNNNNNCTCC	4 cut(s)	4900, 4930, 5415, 5445
Bsc4I	CCNNNNNNNGG	4 cut(s)	1135, 2661, 5566, 7151
Bse118I	RCCGGY	2 cut(s)	154, 6958
Bse21I	CCTNAGG	2 cut(s)	627, 6432
Bse3DI	GCAATG	1 cut(s)	3912
Bse8I	GATNNNNATC	4 cut(s)	573, 4725, 6065, 6261
BseAI	TCCGGA	5 cut(s)	64, 442, 3333, 5896, 6840
BseJI	GATNNNNATC	4 cut(s)	573, 4725, 6065, 6261
BseLI	CCNNNNNNNGG	4 cut(s)	1135, 2661, 5566, 7151
BseMI	GCAATG	1 cut(s)	3912
BseRI	GAGGAG	8 cut(s)	27, 491, 514, 4921, 5381, 5513, 6320, 6708
BseSI	GKGCMC	2 cut(s)	2313, 6989
BseYI	CCCAGC	3 cut(s)	250, 3248, 3840
BsgI	GTGCAG	5 cut(s)	1802, 1974, 2370, 3991, 4699
Bsh1236I	CGCG	4 cut(s)	1470, 1491, 3013, 6512
BshNI	GGYRCC	3 cut(s)	308, 1629, 5588
BsiHKAI	GWGCWC	3 cut(s)	2134, 5206, 6596
BsiHKCI	CYCGRG	1 cut(s)	578
BslFI	GGGAC	7 cut(s)	433, 754, 1252, 1267, 1336, 2006, 3984
BslI	CCNNNNNNNGG	4 cut(s)	1135, 2661, 5566, 7151
BsmBI	CGTCTC	1 cut(s)	1914
BsmFI	GGGAC	7 cut(s)	433, 754, 1252, 1267, 1336, 2006, 3984
BsmI	GAATGC	2 cut(s)	6218, 6622
Bso31I	GGTCTC	3 cut(s)	613, 1259, 2522
BsoBI	CYCGRG	1 cut(s)	578
Bsp119I	TTCGAA	3 cut(s)	5671, 6414, 7175
Bsp120I	GGGCCC	1 cut(s)	6985
Bsp1286I	GDGCHC	7 cut(s)	2134, 2313, 2407, 5206, 5754, 6596, 6989
Bsp13I	TCCGGA	5 cut(s)	64, 442, 3333, 5896, 6840
Bsp1407I	TGTACA	1 cut(s)	6854
Bsp1720I	GCTNAGC	2 cut(s)	351, 5152
Bsp19I	CCATGG	1 cut(s)	3602
Bsp68I	TCGCGA	2 cut(s)	1470, 1491
BspEI	TCCGGA	5 cut(s)	64, 442, 3333, 5896, 6840
BspFNI	CGCG	4 cut(s)	1470, 1491, 3013, 6512
BspHI	TCATGA	6 cut(s)	1405, 3535, 4557, 5158, 5260, 6769
BspMAI	CTGCAG	2 cut(s)	3580, 5874
BspMI	ACCTGC	2 cut(s)	5299, 7390
BspQI	GCTCTTC	2 cut(s)	1962, 6322
BspT104I	TTCGAA	3 cut(s)	5671, 6414, 7175
BspT107I	GGYRCC	3 cut(s)	308, 1629, 5588
BspTNI	GGTCTC	3 cut(s)	613, 1259, 2522
BsrBI	CCGCTC	3 cut(s)	1030, 1264, 1669
BsrDI	GCAATG	1 cut(s)	3912
BsrFI	RCCGGY	2 cut(s)	154, 6958
BsrGI	TGTACA	1 cut(s)	6854
BssAI	RCCGGY	2 cut(s)	154, 6958
BssNI	GRCGYC	1 cut(s)	4889
BssSI	CACGAG	3 cut(s)	613, 3024, 6007
BssT1I	CCWWGG	9 cut(s)	207, 312, 1625, 3602, 3993, 4590, 5928, 7256, 7263
Bst2BI	CACGAG	3 cut(s)	613, 3024, 6007
BstACI	GRCGYC	1 cut(s)	4889
BstAPI	GCANNNNNTGC	3 cut(s)	2357, 3496, 5987
BstAUI	TGTACA	1 cut(s)	6854
BstBAI	YACGTR	5 cut(s)	1274, 1427, 1679, 4226, 6080
BstBI	TTCGAA	3 cut(s)	5671, 6414, 7175
BstDSI	CCRYGG	4 cut(s)	1794, 2989, 3011, 3602
BstEII	GGTNACC	3 cut(s)	1671, 1682, 3128
BstENI	CCTNNNNNAGG	1 cut(s)	5564
BstFNI	CGCG	4 cut(s)	1470, 1491, 3013, 6512
BstHHI	GCGC	4 cut(s)	79, 1320, 1746, 1765
BstNSI	RCATGY	8 cut(s)	24, 28, 1445, 2021, 2976, 3525, 3649, 3863
BstPAI	GACNNNNGTC	1 cut(s)	1308
BstPI	GGTNACC	3 cut(s)	1671, 1682, 3128
BstSFI	CTRYAG	5 cut(s)	697, 3284, 3576, 5464, 5870
BstSLI	GKGCMC	2 cut(s)	2313, 6989
BstSNI	TACGTA	1 cut(s)	6080
BstUI	CGCG	4 cut(s)	1470, 1491, 3013, 6512
BstV2I	GAAGAC	7 cut(s)	77, 1206, 2724, 2805, 3723, 3813, 4785
BstXI	CCANNNNNNTGG	2 cut(s)	666, 4774
Bsu36I	CCTNAGG	2 cut(s)	627, 6432
BsuI	GTATCC	2 cut(s)	795, 5080
BtgI	CCRYGG	4 cut(s)	1794, 2989, 3011, 3602
BtgZI	GCGATG	3 cut(s)	21, 522, 3114
BtrI	CACGTC	1 cut(s)	1511
BtsI	GCAGTG	3 cut(s)	946, 1849, 3331
BtuMI	TCGCGA	2 cut(s)	1470, 1491
BveI	ACCTGC	2 cut(s)	5299, 7390
CaiI	CAGNNNCTG	3 cut(s)	4198, 5177, 6302
CciI	TCATGA	6 cut(s)	1405, 3535, 4557, 5158, 5260, 6769
CfoI	GCGC	4 cut(s)	79, 1320, 1746, 1765
Cfr10I	RCCGGY	2 cut(s)	154, 6958
Cfr42I	CCGCGG	1 cut(s)	3014
CseI	GACGC	3 cut(s)	3734, 6518, 6884
CspCI	CAANNNNNGTGG	3 cut(s)	6963, 6998, 7382
DraI	TTTAAA	2 cut(s)	1068, 6444
DraIII	CACNNNGTG	3 cut(s)	1679, 1682, 6915
EaeI	YGGCCR	4 cut(s)	684, 868, 1360, 1381
EciI	GGCGGA	1 cut(s)	469
Ecl136II	GAGCTC	3 cut(s)	2132, 5204, 6594
Eco105I	TACGTA	1 cut(s)	6080
Eco130I	CCWWGG	9 cut(s)	207, 312, 1625, 3602, 3993, 4590, 5928, 7256, 7263
Eco147I	AGGCCT	1 cut(s)	3290
Eco24I	GRGCYC	6 cut(s)	2134, 2407, 5206, 5754, 6596, 6989
Eco31I	GGTCTC	3 cut(s)	613, 1259, 2522
Eco32I	GATATC	2 cut(s)	4666, 7150
Eco53kI	GAGCTC	3 cut(s)	2132, 5204, 6594
Eco72I	CACGTG	3 cut(s)	1274, 1679, 4226
Eco81I	CCTNAGG	2 cut(s)	627, 6432
Eco88I	CYCGRG	1 cut(s)	578
Eco91I	GGTNACC	3 cut(s)	1671, 1682, 3128
EcoICRI	GAGCTC	3 cut(s)	2132, 5204, 6594
EcoNI	CCTNNNNNAGG	1 cut(s)	5564
EcoO109I	RGGNCCY	5 cut(s)	1281, 1350, 3263, 3998, 6986
EcoO65I	GGTNACC	3 cut(s)	1671, 1682, 3128
EcoRI	GAATTC	2 cut(s)	605, 6249
EcoRV	GATATC	2 cut(s)	4666, 7150
EcoT14I	CCWWGG	9 cut(s)	207, 312, 1625, 3602, 3993, 4590, 5928, 7256, 7263
EcoT22I	ATGCAT	5 cut(s)	26, 2974, 4957, 5976, 6907
EcoT38I	GRGCYC	6 cut(s)	2134, 2407, 5206, 5754, 6596, 6989
ErhI	CCWWGG	9 cut(s)	207, 312, 1625, 3602, 3993, 4590, 5928, 7256, 7263
Esp3I	CGTCTC	1 cut(s)	1914
FalI	AAGNNNNNCTT	8 cut(s)	3028, 3060, 4101, 4133, 5532, 5564, 6437, 6469
FaqI	GGGAC	7 cut(s)	433, 754, 1252, 1267, 1336, 2006, 3984
FauI	CCCGC	2 cut(s)	724, 1054
FauNDI	CATATG	7 cut(s)	1336, 1378, 1399, 1462, 1483, 1504, 1525
FbaI	TGATCA	5 cut(s)	1294, 1588, 2548, 3298, 4815
FblI	GTMKAC	2 cut(s)	921, 3981
FriOI	GRGCYC	6 cut(s)	2134, 2407, 5206, 5754, 6596, 6989
GlaI	GCGC	4 cut(s)	78, 1319, 1745, 1764
GsaI	CCCAGC	3 cut(s)	254, 3252, 3844
GsuI	CTGGAG	5 cut(s)	1574, 2250, 3958, 4737, 6653
HgaI	GACGC	3 cut(s)	3734, 6518, 6884
HhaI	GCGC	4 cut(s)	79, 1320, 1746, 1765
Hin1I	GRCGYC	1 cut(s)	4889
Hin6I	GCGC	4 cut(s)	77, 1318, 1744, 1763
HinP1I	GCGC	4 cut(s)	77, 1318, 1744, 1763
HincII	GTYRAC	2 cut(s)	2613, 5123
HindII	GTYRAC	2 cut(s)	2613, 5123
HindIII	AAGCTT	6 cut(s)	1079, 3530, 5221, 5339, 6279, 6425
Hpy99I	CGWCG	1 cut(s)	371
Hsp92I	GRCGYC	1 cut(s)	4889
HspAI	GCGC	4 cut(s)	77, 1318, 1744, 1763
KflI	GGGWCCC	1 cut(s)	3998
Kpn2I	TCCGGA	5 cut(s)	64, 442, 3333, 5896, 6840
KpnI	GGTACC	2 cut(s)	1633, 5592
Ksp22I	TGATCA	5 cut(s)	1294, 1588, 2548, 3298, 4815
KspI	CCGCGG	1 cut(s)	3014
LguI	GCTCTTC	2 cut(s)	1962, 6322
MbiI	CCGCTC	3 cut(s)	1030, 1264, 1669
MfeI	CAATTG	3 cut(s)	3212, 6343, 6734
MhlI	GDGCHC	7 cut(s)	2134, 2313, 2407, 5206, 5754, 6596, 6989
MlsI	TGGCCA	2 cut(s)	686, 870
MluNI	TGGCCA	2 cut(s)	686, 870
MmeI	TCCRAC	3 cut(s)	2434, 3615, 5484
Mox20I	TGGCCA	2 cut(s)	686, 870
Mph1103I	ATGCAT	5 cut(s)	26, 2974, 4957, 5976, 6907
MroI	TCCGGA	5 cut(s)	64, 442, 3333, 5896, 6840
MroXI	GAANNNNTTC	7 cut(s)	438, 1156, 4113, 5256, 5637, 6473, 6702
MscI	TGGCCA	2 cut(s)	686, 870
MslI	CAYNNNNRTG	8 cut(s)	1446, 1950, 2535, 2585, 3324, 4022, 5265, 6900
Msp20I	TGGCCA	2 cut(s)	686, 870
MspA1I	CMGCKG	1 cut(s)	3013
MunI	CAATTG	3 cut(s)	3212, 6343, 6734
Mva1269I	GAATGC	2 cut(s)	6218, 6622
MvnI	CGCG	4 cut(s)	1470, 1491, 3013, 6512
NciI	CCSGG	5 cut(s)	1285, 1688, 2340, 4403, 6185
NcoI	CCATGG	1 cut(s)	3602
NdeI	CATATG	7 cut(s)	1336, 1378, 1399, 1462, 1483, 1504, 1525
NmeAIII	GCCGAG	4 cut(s)	349, 4664, 5733, 6847
NruI	TCGCGA	2 cut(s)	1470, 1491
NsiI	ATGCAT	5 cut(s)	26, 2974, 4957, 5976, 6907
NspI	RCATGY	8 cut(s)	24, 28, 1445, 2021, 2976, 3525, 3649, 3863
NspV	TTCGAA	3 cut(s)	5671, 6414, 7175
PaeI	GCATGC	2 cut(s)	24, 28
PagI	TCATGA	6 cut(s)	1405, 3535, 4557, 5158, 5260, 6769
PceI	AGGCCT	1 cut(s)	3290
PciI	ACATGT	5 cut(s)	1441, 2017, 3521, 3645, 3859
PciSI	GCTCTTC	2 cut(s)	1962, 6322
PctI	GAATGC	2 cut(s)	6218, 6622
PdmI	GAANNNNTTC	7 cut(s)	438, 1156, 4113, 5256, 5637, 6473, 6702
PmaCI	CACGTG	3 cut(s)	1274, 1679, 4226
PmlI	CACGTG	3 cut(s)	1274, 1679, 4226
Ppu21I	YACGTR	5 cut(s)	1274, 1427, 1679, 4226, 6080
PpuMI	RGGWCCY	4 cut(s)	1281, 1350, 3263, 3998
PscI	ACATGT	5 cut(s)	1441, 2017, 3521, 3645, 3859
PshAI	GACNNNNGTC	1 cut(s)	1308
PshBI	ATTAAT	5 cut(s)	1857, 3609, 4059, 4725, 4746
PsiI	TTATAA	3 cut(s)	2927, 3558, 4515
Psp124BI	GAGCTC	3 cut(s)	2134, 5206, 6596
Psp1406I	AACGTT	1 cut(s)	4420
Psp5II	RGGWCCY	4 cut(s)	1281, 1350, 3263, 3998
PspCI	CACGTG	3 cut(s)	1274, 1679, 4226
PspEI	GGTNACC	3 cut(s)	1671, 1682, 3128
PspFI	CCCAGC	3 cut(s)	250, 3248, 3840
PspOMI	GGGCCC	1 cut(s)	6985
PspPPI	RGGWCCY	4 cut(s)	1281, 1350, 3263, 3998
PstI	CTGCAG	2 cut(s)	3580, 5874
PstNI	CAGNNNCTG	3 cut(s)	4198, 5177, 6302
RruI	TCGCGA	2 cut(s)	1470, 1491
RseI	CAYNNNNRTG	8 cut(s)	1446, 1950, 2535, 2585, 3324, 4022, 5265, 6900
SacI	GAGCTC	3 cut(s)	2134, 5206, 6596
SacII	CCGCGG	1 cut(s)	3014
SapI	GCTCTTC	2 cut(s)	1962, 6322
ScaI	AGTACT	3 cut(s)	850, 1184, 5077
SduI	GDGCHC	7 cut(s)	2134, 2313, 2407, 5206, 5754, 6596, 6989
SfcI	CTRYAG	5 cut(s)	697, 3284, 3576, 5464, 5870
Sfr303I	CCGCGG	1 cut(s)	3014
SfuI	TTCGAA	3 cut(s)	5671, 6414, 7175
SgrBI	CCGCGG	1 cut(s)	3014
SmiMI	CAYNNNNRTG	8 cut(s)	1446, 1950, 2535, 2585, 3324, 4022, 5265, 6900
SmlI	CTYRAG	5 cut(s)	2523, 3421, 3814, 6491, 6749
SmoI	CTYRAG	5 cut(s)	2523, 3421, 3814, 6491, 6749
SnaBI	TACGTA	1 cut(s)	6080
SpeI	ACTAGT	1 cut(s)	2254
SphI	GCATGC	2 cut(s)	24, 28
SseBI	AGGCCT	1 cut(s)	3290
SstI	GAGCTC	3 cut(s)	2134, 5206, 6596
StuI	AGGCCT	1 cut(s)	3290
StyI	CCWWGG	9 cut(s)	207, 312, 1625, 3602, 3993, 4590, 5928, 7256, 7263
TaqII	GACCGA	2 cut(s)	3048, 3147
TatI	WGTACW	8 cut(s)	848, 933, 945, 1182, 3648, 4356, 5075, 6854
TauI	GCSGC	3 cut(s)	1722, 3013, 5623
TspGWI	ACGGA	5 cut(s)	1407, 1588, 1817, 6143, 6586
VspI	ATTAAT	5 cut(s)	1857, 3609, 4059, 4725, 4746
XagI	CCTNNNNNAGG	1 cut(s)	5564
XbaI	TCTAGA	4 cut(s)	1342, 2371, 2735, 5434
XceI	RCATGY	8 cut(s)	24, 28, 1445, 2021, 2976, 3525, 3649, 3863
XcmI	CCANNNNNNNNNTGG	2 cut(s)	727, 5221
XmaJI	CCTAGG	1 cut(s)	1625
XmiI	GTMKAC	2 cut(s)	921, 3981
XmnI	GAANNNNTTC	7 cut(s)	438, 1156, 4113, 5256, 5637, 6473, 6702
ZraI	GACGTC	1 cut(s)	4890
ZrmI	AGTACT	3 cut(s)	850, 1184, 5077
Zsp2I	ATGCAT	5 cut(s)	26, 2974, 4957, 5976, 6907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.