Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0013012

Physical Location & Seq

Genomic Coordinates Reverse (-)

72340076 .. 72353310

Gene Structure 13235 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G045430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7437 bp

ATGGGCGATGGAGGAGTCGCATGCATGCCTTTGCAGCATAATATCATGGACAGGTTTCCAATTCCGGAGAAGACTGCGCTTTGCGGAGGCAATACTAATACTAATGCTGCTACTACTACTACTACCACCGCTAACAATGGCTTCAATTCCAAGCCGGTTGTTAAGAAGAAGGTAATGAAGATCAAGAAGAGGATTGTCAGGAAGCCCAAGGCAAAAAGCAACAGTAACGTAGAGTCGGGGAAGAGTGAGCTGGGATTGGTTAAAGGTGAGAAGAGTGATACTACTAAGGAAGCAGAGAACAGTGGTGGTGCCAAGGAAGCAGCCGAGAAGAGTGGTGTGAAGGAAGCTGTAAAGGAAGCTGAGAACAGTGCTGCTCAGCAAGTTGAGAACGTCGAATATAATGGTGAGGATAAGAAGGAGGAAGTGGAAGAGGGTGAATTGGGGACTTTGAATGGTGAATTTGTTCCGGACAAGTGGCGGAGAAGCGAGGTTGAGAAGGAGGAGATTGGGGGTGAGAAGTGGAGGAGAAGCGAGGTGGAGAAGGGAGAGTCTTTTTCTGGGAAATGGCGTAGAGGTGATGCAGAGAAGGCAGATATATATCCCGAGAAGAGTAGAAATAGTGAAGCTGAATTCGGCTCGTGGAGACCACCCAAGGATGAAATTGAAAGAGGTGAGTATATTCCAGATAGATGGCAAAAAGGAGAGCTGGCCAGAGATGACTACAGTAACCGAAAAATGCCCGCCAGGTACGATATGGGTGGGAGGGGCAGGGGAAGGGGCTGGAAATTTGAGTCCCCTTCTGGCAAGTATCCAAATGATGACGGGTTTAGAAGGAAAGAATATAGTAGAGGTGGGGGTCAGCACAGCAAGAGTACTTTCAGGTGGGATAGTGGCCAGGAGAGGAATACGAGGATCAGTTCCAAAATTGTTGATGATGAGGGTGTCTACAAGAATGAGTACAGCAGTGAGTACAGCAATGGTAAGTATCATGCAAAGGATTACTCTTCTGTTAATCGGTTGAAGAGGTATGGTCCTGATTCAAATAGTAGTGAGCGGAAGCACTATGGAGATTATGGGGATTATGCGGGTTTAAAAAGTCGCAAGCTTTCTGATGAAAGTAATCGCTCTGCTCACTCTGAGCATTATTCACACCGTTCTGGGGAGAGATCTTATCGAAATCCTTCTTTTAGACCAGCATCAGACAAGTACTCTTCCAGGCACTATGAACCTACTTTGTCTTCCAGAGTGGTTTATGACAGGCATGGGCGAAGTCCAGGTCTCTCTGAGCGGTCCCCACGTGACAGGTCCCGGTATTATGATCACCTGGACCGGAGTCCAGTGCGCCGTGAGAGATCTCCATATGATCTAGAGAGGTCCCCTTATGGCCGTGAGAAATCACCATATGGCCGTGAGAGATCTCCATATGGTCATGAGAAATCTCCATATGTACGTGATAAATCTCCACATGTTCGTGACAGATCTCCATATGTTCGCGACAAGTCTCCATATGCTCGCGAGAAGTCACCATATGGACGTGAAAGATCTCCATATGTTCGTGACAGATCTCCTTATGTTCTGGAGAGATCTCCATACGAACGGAGCCGTAATTATGATCACAGAAACCGCAGTTTGTCTCCACAAGATCGACCTAGGTACCATGATCGCAGGGATCACACCCCAAACTATTTGGAGCGGTCACCACGTGGTGACCGGGGTAGAGCTTCTAGTTATAGAGGTACAGGCCGCAAAAGTGGAGCAAGTGATAGGCGCAACTCCCAATATAAAGCGCACGAAGATAAGCTGGTGCAGAAGGATCCCACGGGAACGGACTCAAATTCCTCTGCCAAAGAATGTCAGGATAGAAGCAGTGTTCTTGACATTAATGGAACTGTGGAGACAAACACCAATTCTGAGTCTCACAAAGAAGAACCGTCTCAAAGTCCAAATATAAATTGCAAAGAAACTTCTCATATTAGTGCAGCCCCTCCTGAAGAGCTGCCTTCTATGGAAGAAGATATGGATATATGCGACACACCACCACATGTCCCAGTGATTGCTGATTCATCCACAGGGAAATGGTTTTACCTTGATTATTATGGTGTGGAATGTGGGCCTTCCAAATTATGTGAGCTTAAAGCACTTGTTGAAGAAGGAGCTCTGATGTCCGATCACATGGTCAAGCACTCAGACAGTGATAGGTGGGTAACTGTTGAAAATGCAGTTTCGCCATTGATTACCGTAAATTTTCCATCCATTGTATCGGACTCGATAACTGGACTAGTAAGCCCTCCAGAAGCTCCTGGCAATCTATTGGCAGATATTGGACATACTGGGCACTATGGTACTCAGAGTGGGAGTTTACCGGGGTTGTGTGCAGATGTTGCTTCTGAGCCTCTAGAAGATTTGCACATTGAAGAAAGGGTTGGGGCTCTGATGGAAGGTTTAACAGTTATTCCTGGCAGGGAACTTGAAGCTGTTGGAGAAGTTCTGCAAATGTCATTTGAATCTGCTCAACTGGAAGTATGGGGAAACAGTGAAGGTCTCTCTCAAGGTCATATCGGTGAACAGAATGATCAGAAAACTGAGGAACCACGATTTTCTGACATTAAAATGAAAGAAGCAGCAGAAATAGGGTTGACTTTACCCTCTGACAAAGATGCTTTTGCTTGTGGTGATTCCGGTGACTGGTTTTCTGGTCGATGGTCATGCAAGGGTGGTGATTGGAAAAGGAACGAAGAAGGTGTGCAAGATAGGTCTTCTAGAAAGAAACTTGTTGTCAATGATGGTTTCCCATTATGCCAGATGTCAAAATCTGGGTATGAAGACCCTCGATGGCAAAGAAAAGATGAACTGTATTATCCTTCACAGAACAGAAGGCTTGATCTACCTACTTGGGCTTTTTCATGTCCAGATGATGCTAATAGAGTGTCTCAAAGTAAGCCAACTGTTATAAAAGGAGTAAAAGGAACTATCCTTCCGGTAGTCAAGATAAATGCATGTGTTGTAAAAGACCACGGTTCATTTGTTTCTGAGCCGCGGATAAAAGCACGAGGAATGGAAAGGCATCCTTCAAGGTCTGCTCGGTCATATTCCGCAAGTAGTGATGGCAAGAGATCATCTGGCGATGGTGATTCTCAAATGAAACCAGTAGGTGACCGAGGCTCAAAGGGATCTTCCAAATGCGTTTCCTCCATTAAAATCCCCAAAGACCGTATTGGCACAGTTGATGACTTGCAATTGCATTTGGGAGACTGGTACTACCTTGATGGTGCTGGGCATGAACGAGGACCTTCATCATTCTCTGAGCTACAGGCCTTAGTTGATCAAGGAGTGATCCTGAAACATAGCAGTGTGTTCCGGAAATTTGATAAAGTCTGGGTTCCAGTTACCTCTGCCATTGAGACTTCTGATGCTACCAAAATGAATAAGGAGAAGAACAGAACCTCAAGTAATTTTTCAGGGCAATCTCAAAGTGCTACACTTGCAGAATCAAAAACAAATTTGAGTTGGTTGCAGAATCTGCACCCTCAGTTCATTGGTTATACATGTGGAAAGCTTCATGAATTGGTGATGAAATCTTATAAAAGTCGGGAGTTTGCTGCAGCCATAAATGAGGTTTTAGATCCATGGATTAATGCAAGGCAGCCAAAAAAAGAGTTGGACAAGCACATGTACTGGAAAGCAGATGGTGATGCACGTTCTTCTAAAAGAACTCGGTTGCTGGTTGATGAAAGTGAAGAAGACTATGACGCAGAAGAGGACTTGCAAACAATAGAAAAGGATGATTCCGCATTTGAGGATCTAATTGGTAATGCTTCTTTCTTCAGAGAAGACAGCTTGAGTTATGGATCAGAGATGGCAAGCTGGGGTTTATTGGATGGACATGTGCTGGCACGGGTCTTTCATTTTTTAAGATTGGACATGAAATCTCTTACCATTGCTTCTCTGACTTGTAAGCACTGGAGAGCTGCGGTCAGTTTTTACAAGGATATTTCAAGACAGGTAGACTTGTCATCCTTGGGTCCCAACTGCACCGACTCCATGATTGTGAAGATTATGAGTGGTTATGGCAAAGAGAAGATTAATTCTATGGTTCTAATTGGTTGCACAAACATCACTCCCCACACTCTTGAAGAGATTCTTGGTTCACTTCCTTGTCTATCTACCATAGATATTAGGGGTTGCAACCAGTTTGGTGAGTTGGTCATTAAATTTCAGAACCTGAACTGGATTAAGAGCCGAAGCTCACGTGGTACAAAGATACATGATGAATCCAATTCAAAATTAAGGAGTCTGAAATATATCAGTGAGAAATCTTCTTATGTTTCGAGAAGTAAGGTTCTGGGTAATGATATGGATGATTTTAGTGAACTCAAAGTGTACTTTGATAGTGTGGATAAGAGAGACTCAGCTAATCAATCATTCCGGGGAAGTTTATACAAACGTTCAAAACTATTTGATGCTAGACGGTCCTCATCCATTCTATCTAGGGATGCTCGTATGAGGCGGTTGTCCATTAAGAAATCAGAAAATGGTTATAAGAGGATGGAGGAATTTGTTGCTTCAAGTCTGAAGGACATCATGAAGGAGAATACATCTGATTTCTTTGTACCCAAGGTTGCTGAAATTCAAGATAGAATGAGAAATGGGTATTACATTCGCCGAGGATTGAGTTCTGTGAAGGAGGATATCAGTCGAATGTGCAGGGATGCAATTAAAGCAAAGAATCGTGGTGAAGCTGGAGATATTAATCACCTGAATCACATTATTAATTTGTTTATACAACTTGCCACACGTTTGGAAGGGGCTTCCAAGTCTTCTGATGCAAGGGATGAGCTGATCAAGTCGTGGGAAGATGATACATTTGCAGGGGTTTCTTCTGCCTCCAAGAGTAAAAAGAAGCTCAATAAGACGTCTGAAAGGAAGTTGAGGAGTAATGGTTCCTTTATGAATGGTAGTGTGGATAATGGAGAATATGCATCTGATCGAGAAATCAGAAGGCGTTTATCCAAGTTGAATAAAAAATCTATGGATTCAGAAAGTGAAACATCTGATGATCTTGATAGGTCATCTGAAGATAGCAAGAGCAATACTGAGAGTACTGCCTCGGATACAGAAAGTGACTTGGAATCTGGGTCACAGAGTCAACCTGGGCAATCAACAGCAGATGGATGCTTAGCTCATGATGAGGGTTCAGATTCTGCGACTGATGATCGTGAGTGGGGAGCTCGCATGACCAAATCAAGCTTGGTTCCTCCTGTTACGAGAAAATATGAAGTCATTCATGAATATGTTATTGTATCGCATGAAGAGGATGTAAAGCGGAAGATGCAGGTATCTTTGCCGGATGACTATGTTGAGAAGCTTTATTCGCAGAAGAATGGAACTGAGGAGTCTGATATGGAACTTCCTGAAGTCAAGGACTACAAGCCCAGGAAAATGCTTGGAGATGAGGTTCTAGAGCAAGAGGTTTATGGAATTGATCCCTACAGCCATAATCTTTTACTTGATTCCATGCCAGAGGAGTTGGATTGGCCTCTTTTAGAGAAGCATCTGTTTATAGAAGATGTGCTTCTTCGCACCCTGAATAAGGAAGTTAGACACTTTACTGGTACCGGAACTACTCCCATGATCTATCCTTTGCGGCCCGTTGTTGAAGATATTCGGAAAACTGCTGAAGAGGATAGTGATATTCGAACTGTGAGAATGTGCCAGGGTATTTTGAAGGCCATAGACAGTCGCCCTGATGATAAATATGTTGCTTATAGAAAGGGGCTCGGCGTTGTTTGCAACAAAGAAGAAGGTTTTGGAGAAGAAGATTTTGTTGTGGAATTTTTGGGGGAGGTATATCCTGTTTGGAAATGGTATGAGAAGCAAGATGGGATTCGTTCTCTGCAGAAAAACAGTAAAGATCCAGCTCCGGAATTTTACAACATTTATCTTGAAAGGCCCAAGGGTGATGCTGATGGGTATGATTTAGTTGTTGTTGATGCCATGCATAAAGCAAACTATGCAAGTAGAATTTGTCACTCGTGCCGACCTAATTGTGAAGCAAAAGTTACTGCTGTCGATGGTCGTTACCAGATCGGTATCTACACTGTACGTAAAATTCAATATGGTGAAGAGATCACATTTGACTACAACTCTGTTACGGAGAGTAAGGAAGAATATGAAGCATCAGTTTGTTTGTGTGGCAGTCAAGTTTGCCGGGGAAGTTACCTGAATTTGACAGGCGAAGGAGCATTCCTGAAGGTGCTGAAGGATTGGCATGGCATTCTGGATCGTCATCATCTAATGCTGGAAGCTTGCGAGTTAAATTCAGTATCTGAGGAGGATTATCTTGACCTGGGAAGAGCTGGTTTAGGCAATTGTTTGCTTGGTGGGTTGCCAGATTGGGTGATTGCATATTCAGCTCGTTTGGTGAGGTTCATAAACTTCGAAAGAACAAAGCTTCCTGAGGTGATTTTAAAGCACAACTTGGAAGAAAAGAGGAAGTATTTCTCAGATATATGTCTTGAGGTTGAGAAGAGTGACGCGGAGGTTCAGGCTGAGGGTGTGTTCAACCAAAGGCTTCAGAATTTGGCTGTTACTCTTGACAAGGTGAGGTATGTTATGAGCTCCGTATTTGGCAACCCAAAGAATGCTCCACCTCCACTGGAGAGGTTAAGTGCAGAAGAAGCTGTTGGCTTTGTATGGAAGGGAGAAGGATCAGTTGTTGAGGAACTCCTTCAGAGCATGGCTCCTCATGTGGAGGAACAATTGCTAAATGATCTCAAGTCCAAGATGCTTGCTCATGATCCATCGAGTTCAGATGACATTTGGAAAGAACTTAAAAGATCGTTACTATGGTTGAGAGATGAGGTCCGGAATCTTCCATGTACATACAAGTCTCGGCATGACGCTGCTGCTGACTTGATCCATATTTATGCATACACAAGGTGCTACATTAGAATACGGGAATACAAATCAGTAACTTCACCGCCGGTTTACATTAGTCCACTTGACTTGGGCCCCAACTACACTAACAAATCGGGGGCAGATTTTCAGGAGTATTGCAAGACTTACGGGGAGAATTATTGTTTAGGGCAGCTATTTTTTTGGTATAATCAGTCTAATGCAGAGCCAGATTGTAGTATGGCTAGAGCAAGTAGGGGTTGCTTATCATTACCTGATATCGGTTCCTTCTATGCCAAGCTTTCGAAACCTACACGGCAGCGTGTTTATGGTCCAAAGGCTGTCAAATTTATGCTTTCAAGGATGGAGAAGCAGCCCCAAAAGCCTTGGCCCAAGGATCGAATATGGTCATTTAATAGCTCCCCAAAAGTTCTTGGCAGTCCAATGCTAGATGCTGTTGTGAATAACTCTTTCTTGGACAAAGAAATGTTGCATTGGTTAAAGCACAGACCTGCAATATATCAAGCTATGTGGGATCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009791 GO:0009908 GO:0009987 GO:0010228 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019538 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0034968 GO:0036211 GO:0042054 GO:0042800 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048443 GO:0048466 GO:0048467 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0051276 GO:0051568 GO:0061458 GO:0071704 GO:0071840 GO:0090567 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SET

Protein Analysis

2478

Amino Acids

280.78

Weight (kDa)

6.55

Isoelectric Point (pI)

53.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GYF_ATXR3	PF25531	691 - 738	2.1e-29	Histone-lysine N-methyltransferase ATXR3, GYF domain
GYF_ATXR3	PF25531	1083 - 1114	7.9e-08	Histone-lysine N-methyltransferase ATXR3, GYF domain
SET	PF00856	1840 - 2048	1.2e-12	SET domain
SDG2_C	PF19633	2059 - 2478	1.7e-235	Protein SET DOMAIN GROUP 2 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013012)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15180
fragaria_vesca	FvH4_6g43760
malus_domestica	MD09G1103200.v1.1 MD17G1091000.v1.1
prunus_persica	Prupe.3G222600_v2.0.a1
pyrus_communis	pycom09g02660 pycom17g08780
rosa_chinensis	RchiOBHm_Chr2g0160501
rosa_laevigata	RLG00000021218
rosa_multiflora	Rmu_sc0000674.1_g000001
rosa_roxburghii	Rroxscaffold_2G00089780
rosa_rugosa	Rorug02G0483400 Rorug02G0483400
rosa_samantha	Rh2AG548700 Rh2BG562400 Rh2CG533000 Rh2DG571100
rosa_wichuraiana	Rw2G045430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	2951, 3582, 4539
AatII	GACGTC	1 cut(s)	4916
Acc36I	ACCTGC	2 cut(s)	5323, 7414
Acc65I	GGTACC	2 cut(s)	1653, 5612
AccB1I	GGYRCC	3 cut(s)	308, 1653, 5612
AccBSI	CCGCTC	3 cut(s)	1054, 1288, 1693
AccI	GTMKAC	2 cut(s)	945, 4005
AccII	CGCG	4 cut(s)	1494, 1515, 3037, 6536
AccIII	TCCGGA	5 cut(s)	64, 466, 3357, 5920, 6864
AclI	AACGTT	1 cut(s)	4444
AcoI	YGGCCR	4 cut(s)	708, 892, 1384, 1405
AcvI	CACGTG	3 cut(s)	1298, 1703, 4250
AcyI	GRCGYC	1 cut(s)	4913
AdeI	CACNNNGTG	3 cut(s)	1703, 1706, 6939
AfiI	CCNNNNNNNGG	4 cut(s)	1159, 2685, 5590, 7175
AflIII	ACRYGT	6 cut(s)	1465, 2041, 3545, 3669, 3883, 4793
AhlI	ACTAGT	1 cut(s)	2278
AjiI	CACGTC	1 cut(s)	1535
AjuI	GAANNNNNNNTTGG	8 cut(s)	2406, 2438, 4125, 4157, 5790, 5822, 6666, 6698
AloI	GAACNNNNNNTCC	8 cut(s)	2959, 2991, 4924, 4956, 5439, 5471, 6436, 6468
Alw21I	GWGCWC	3 cut(s)	2158, 5230, 6620
AlwNI	CAGNNNCTG	4 cut(s)	3520, 4222, 5201, 6326
Ama87I	CYCGRG	1 cut(s)	602
Aor13HI	TCCGGA	5 cut(s)	64, 466, 3357, 5920, 6864
ApaI	GGGCCC	1 cut(s)	7013
ArsI	GACNNNNNNTTYG	2 cut(s)	1261, 1293
AseI	ATTAAT	5 cut(s)	1881, 3633, 4083, 4749, 4770
Asp700I	GAANNNNTTC	7 cut(s)	462, 1180, 4137, 5280, 5661, 6497, 6726
Asp718I	GGTACC	2 cut(s)	1653, 5612
AspA2I	CCTAGG	1 cut(s)	1649
AspLEI	GCGC	4 cut(s)	79, 1344, 1770, 1789
AsuC2I	CCSGG	5 cut(s)	1309, 1712, 2364, 4427, 6209
AsuII	TTCGAA	3 cut(s)	5695, 6438, 7199
AvaI	CYCGRG	1 cut(s)	602
AvrII	CCTAGG	1 cut(s)	1649
AxyI	CCTNAGG	1 cut(s)	6456
BaeGI	GKGCMC	2 cut(s)	2337, 7013
BaeI	ACNNNNGTAYC	2 cut(s)	1637, 1670
BalI	TGGCCA	2 cut(s)	710, 894
BamHI	GGATCC	1 cut(s)	1813
BanI	GGYRCC	3 cut(s)	308, 1653, 5612
BanII	GRGCYC	6 cut(s)	2158, 2431, 5230, 5778, 6620, 7013
BauI	CACGAG	3 cut(s)	637, 3048, 6031
BbrPI	CACGTG	3 cut(s)	1298, 1703, 4250
BbsI	GAAGAC	7 cut(s)	77, 1230, 2748, 2829, 3747, 3837, 4809
Bbv12I	GWGCWC	3 cut(s)	2158, 5230, 6620
BbvCI	CCTCAGC	1 cut(s)	6549
BceAI	ACGGC	5 cut(s)	1329, 1371, 1392, 1587, 7226
BcgI	CGANNNNNNTGC	2 cut(s)	2688, 2722
BciVI	GTATCC	2 cut(s)	819, 5104
BclI	TGATCA	5 cut(s)	1318, 1612, 2572, 3322, 4839
BcnI	CCSGG	5 cut(s)	1309, 1712, 2364, 4427, 6209
BcuI	ACTAGT	1 cut(s)	2278
BfmI	CTRYAG	5 cut(s)	721, 3308, 3600, 5488, 5894
BfuAI	ACCTGC	2 cut(s)	5323, 7414
BfuI	GTATCC	2 cut(s)	819, 5104
BglII	AGATCT	7 cut(s)	1166, 1352, 1415, 1478, 1541, 1562, 1583
BlnI	CCTAGG	1 cut(s)	1649
BlpI	GCTNAGC	2 cut(s)	375, 5176
BmcAI	AGTACT	3 cut(s)	874, 1208, 5101
BmeT110I	CYCGRG	1 cut(s)	602
BmgBI	CACGTC	1 cut(s)	1535
BmrI	ACTGGG	2 cut(s)	2042, 2340
BmuI	ACTGGG	2 cut(s)	2042, 2340
BoxI	GACNNNNGTC	1 cut(s)	1332
BpiI	GAAGAC	7 cut(s)	77, 1230, 2748, 2829, 3747, 3837, 4809
BplI	GAGNNNNNCTC	2 cut(s)	6724, 6756
BpmI	CTGGAG	5 cut(s)	1598, 2274, 3982, 4761, 6677
Bpu10I	CCTNAGC	1 cut(s)	6549
Bpu1102I	GCTNAGC	2 cut(s)	375, 5176
Bpu14I	TTCGAA	3 cut(s)	5695, 6438, 7199
BpuEI	CTTGAG	5 cut(s)	2532, 3430, 3859, 6536, 6758
BpuMI	CCSGG	5 cut(s)	1309, 1712, 2364, 4427, 6209
BsaAI	YACGTR	5 cut(s)	1298, 1451, 1703, 4250, 6104
BsaBI	GATNNNNATC	4 cut(s)	597, 4749, 6089, 6285
BsaHI	GRCGYC	1 cut(s)	4913
BsaI	GGTCTC	3 cut(s)	637, 1283, 2546
BsaWI	WCCGGW	9 cut(s)	64, 466, 1329, 2678, 2977, 3357, 5615, 5920, 6864
BsaXI	ACNNNNNCTCC	4 cut(s)	4924, 4954, 5439, 5469
Bsc4I	CCNNNNNNNGG	4 cut(s)	1159, 2685, 5590, 7175
Bse118I	RCCGGY	2 cut(s)	154, 6982
Bse21I	CCTNAGG	1 cut(s)	6456
Bse3DI	GCAATG	2 cut(s)	982, 3936
Bse8I	GATNNNNATC	4 cut(s)	597, 4749, 6089, 6285
BseAI	TCCGGA	5 cut(s)	64, 466, 3357, 5920, 6864
BseJI	GATNNNNATC	4 cut(s)	597, 4749, 6089, 6285
BseLI	CCNNNNNNNGG	4 cut(s)	1159, 2685, 5590, 7175
BseMI	GCAATG	2 cut(s)	982, 3936
BseRI	GAGGAG	8 cut(s)	27, 515, 538, 4945, 5405, 5537, 6344, 6732
BseSI	GKGCMC	2 cut(s)	2337, 7013
BseYI	CCCAGC	3 cut(s)	250, 3272, 3864
BsgI	GTGCAG	7 cut(s)	1826, 1998, 2394, 3506, 4015, 4723, 6690
Bsh1236I	CGCG	4 cut(s)	1494, 1515, 3037, 6536
BshNI	GGYRCC	3 cut(s)	308, 1653, 5612
BsiHKAI	GWGCWC	3 cut(s)	2158, 5230, 6620
BsiHKCI	CYCGRG	1 cut(s)	602
BslFI	GGGAC	7 cut(s)	457, 778, 1276, 1291, 1360, 2030, 4008
BslI	CCNNNNNNNGG	4 cut(s)	1159, 2685, 5590, 7175
BsmBI	CGTCTC	1 cut(s)	1938
BsmFI	GGGAC	7 cut(s)	457, 778, 1276, 1291, 1360, 2030, 4008
BsmI	GAATGC	3 cut(s)	6242, 6273, 6646
Bso31I	GGTCTC	3 cut(s)	637, 1283, 2546
BsoBI	CYCGRG	1 cut(s)	602
Bsp119I	TTCGAA	3 cut(s)	5695, 6438, 7199
Bsp120I	GGGCCC	1 cut(s)	7009
Bsp1286I	GDGCHC	7 cut(s)	2158, 2337, 2431, 5230, 5778, 6620, 7013
Bsp13I	TCCGGA	5 cut(s)	64, 466, 3357, 5920, 6864
Bsp1407I	TGTACA	1 cut(s)	6878
Bsp1720I	GCTNAGC	2 cut(s)	375, 5176
Bsp19I	CCATGG	1 cut(s)	3626
Bsp68I	TCGCGA	2 cut(s)	1494, 1515
BspEI	TCCGGA	5 cut(s)	64, 466, 3357, 5920, 6864
BspFNI	CGCG	4 cut(s)	1494, 1515, 3037, 6536
BspHI	TCATGA	6 cut(s)	1429, 3559, 4581, 5182, 5284, 6793
BspMAI	CTGCAG	2 cut(s)	3604, 5898
BspMI	ACCTGC	2 cut(s)	5323, 7414
BspQI	GCTCTTC	2 cut(s)	1986, 6346
BspT104I	TTCGAA	3 cut(s)	5695, 6438, 7199
BspT107I	GGYRCC	3 cut(s)	308, 1653, 5612
BspTNI	GGTCTC	3 cut(s)	637, 1283, 2546
BsrBI	CCGCTC	3 cut(s)	1054, 1288, 1693
BsrDI	GCAATG	2 cut(s)	982, 3936
BsrFI	RCCGGY	2 cut(s)	154, 6982
BsrGI	TGTACA	1 cut(s)	6878
BssAI	RCCGGY	2 cut(s)	154, 6982
BssNI	GRCGYC	1 cut(s)	4913
BssSI	CACGAG	3 cut(s)	637, 3048, 6031
Bst2BI	CACGAG	3 cut(s)	637, 3048, 6031
BstACI	GRCGYC	1 cut(s)	4913
BstAPI	GCANNNNNTGC	3 cut(s)	2381, 3520, 6011
BstAUI	TGTACA	1 cut(s)	6878
BstBAI	YACGTR	5 cut(s)	1298, 1451, 1703, 4250, 6104
BstBI	TTCGAA	3 cut(s)	5695, 6438, 7199
BstDSI	CCRYGG	4 cut(s)	1818, 3013, 3035, 3626
BstEII	GGTNACC	3 cut(s)	1695, 1706, 3152
BstENI	CCTNNNNNAGG	1 cut(s)	5588
BstFNI	CGCG	4 cut(s)	1494, 1515, 3037, 6536
BstHHI	GCGC	4 cut(s)	79, 1344, 1770, 1789
BstNSI	RCATGY	8 cut(s)	24, 28, 1469, 2045, 3000, 3549, 3673, 3887
BstPAI	GACNNNNGTC	1 cut(s)	1332
BstPI	GGTNACC	3 cut(s)	1695, 1706, 3152
BstSFI	CTRYAG	5 cut(s)	721, 3308, 3600, 5488, 5894
BstSLI	GKGCMC	2 cut(s)	2337, 7013
BstSNI	TACGTA	1 cut(s)	6104
BstUI	CGCG	4 cut(s)	1494, 1515, 3037, 6536
BstV2I	GAAGAC	7 cut(s)	77, 1230, 2748, 2829, 3747, 3837, 4809
BstXI	CCANNNNNNTGG	2 cut(s)	690, 4798
Bsu36I	CCTNAGG	1 cut(s)	6456
BsuI	GTATCC	2 cut(s)	819, 5104
BtgI	CCRYGG	4 cut(s)	1818, 3013, 3035, 3626
BtgZI	GCGATG	2 cut(s)	21, 3138
BtrI	CACGTC	1 cut(s)	1535
BtsI	GCAGTG	3 cut(s)	970, 1873, 3355
BtuMI	TCGCGA	2 cut(s)	1494, 1515
BveI	ACCTGC	2 cut(s)	5323, 7414
CaiI	CAGNNNCTG	4 cut(s)	3520, 4222, 5201, 6326
CciI	TCATGA	6 cut(s)	1429, 3559, 4581, 5182, 5284, 6793
CfoI	GCGC	4 cut(s)	79, 1344, 1770, 1789
Cfr10I	RCCGGY	2 cut(s)	154, 6982
Cfr42I	CCGCGG	1 cut(s)	3038
CseI	GACGC	3 cut(s)	3758, 6542, 6908
CspCI	CAANNNNNGTGG	3 cut(s)	6987, 7022, 7406
DraI	TTTAAA	2 cut(s)	1092, 6468
DraIII	CACNNNGTG	3 cut(s)	1703, 1706, 6939
EaeI	YGGCCR	4 cut(s)	708, 892, 1384, 1405
EciI	GGCGGA	1 cut(s)	493
Ecl136II	GAGCTC	3 cut(s)	2156, 5228, 6618
Eco105I	TACGTA	1 cut(s)	6104
Eco147I	AGGCCT	1 cut(s)	3314
Eco24I	GRGCYC	6 cut(s)	2158, 2431, 5230, 5778, 6620, 7013
Eco31I	GGTCTC	3 cut(s)	637, 1283, 2546
Eco32I	GATATC	2 cut(s)	4690, 7174
Eco53kI	GAGCTC	3 cut(s)	2156, 5228, 6618
Eco72I	CACGTG	3 cut(s)	1298, 1703, 4250
Eco81I	CCTNAGG	1 cut(s)	6456
Eco88I	CYCGRG	1 cut(s)	602
Eco91I	GGTNACC	3 cut(s)	1695, 1706, 3152
EcoICRI	GAGCTC	3 cut(s)	2156, 5228, 6618
EcoNI	CCTNNNNNAGG	1 cut(s)	5588
EcoO109I	RGGNCCY	5 cut(s)	1305, 1374, 3287, 4022, 7010
EcoO65I	GGTNACC	3 cut(s)	1695, 1706, 3152
EcoRI	GAATTC	1 cut(s)	629
EcoRV	GATATC	2 cut(s)	4690, 7174
EcoT22I	ATGCAT	5 cut(s)	26, 2998, 4981, 6000, 6931
EcoT38I	GRGCYC	6 cut(s)	2158, 2431, 5230, 5778, 6620, 7013
Esp3I	CGTCTC	1 cut(s)	1938
FalI	AAGNNNNNCTT	8 cut(s)	3052, 3084, 4125, 4157, 5556, 5588, 6461, 6493
FaqI	GGGAC	7 cut(s)	457, 778, 1276, 1291, 1360, 2030, 4008
FauI	CCCGC	2 cut(s)	748, 1078
FauNDI	CATATG	8 cut(s)	1360, 1402, 1423, 1444, 1486, 1507, 1528, 1549
FbaI	TGATCA	5 cut(s)	1318, 1612, 2572, 3322, 4839
FblI	GTMKAC	2 cut(s)	945, 4005
FriOI	GRGCYC	6 cut(s)	2158, 2431, 5230, 5778, 6620, 7013
GlaI	GCGC	4 cut(s)	78, 1343, 1769, 1788
GsaI	CCCAGC	3 cut(s)	254, 3276, 3868
GsuI	CTGGAG	5 cut(s)	1598, 2274, 3982, 4761, 6677
HgaI	GACGC	3 cut(s)	3758, 6542, 6908
HhaI	GCGC	4 cut(s)	79, 1344, 1770, 1789
Hin1I	GRCGYC	1 cut(s)	4913
Hin6I	GCGC	4 cut(s)	77, 1342, 1768, 1787
HinP1I	GCGC	4 cut(s)	77, 1342, 1768, 1787
HincII	GTYRAC	2 cut(s)	2637, 5147
HindII	GTYRAC	2 cut(s)	2637, 5147
HindIII	AAGCTT	7 cut(s)	1103, 3554, 5245, 5363, 6303, 6449, 7193
Hpy99I	CGWCG	1 cut(s)	395
Hsp92I	GRCGYC	1 cut(s)	4913
HspAI	GCGC	4 cut(s)	77, 1342, 1768, 1787
KflI	GGGWCCC	1 cut(s)	4022
Kpn2I	TCCGGA	5 cut(s)	64, 466, 3357, 5920, 6864
KpnI	GGTACC	2 cut(s)	1657, 5616
Ksp22I	TGATCA	5 cut(s)	1318, 1612, 2572, 3322, 4839
KspI	CCGCGG	1 cut(s)	3038
LguI	GCTCTTC	2 cut(s)	1986, 6346
MbiI	CCGCTC	3 cut(s)	1054, 1288, 1693
MfeI	CAATTG	3 cut(s)	3236, 6367, 6758
MhlI	GDGCHC	7 cut(s)	2158, 2337, 2431, 5230, 5778, 6620, 7013
MlsI	TGGCCA	2 cut(s)	710, 894
MluNI	TGGCCA	2 cut(s)	710, 894
MmeI	TCCRAC	3 cut(s)	2458, 3639, 5508
Mox20I	TGGCCA	2 cut(s)	710, 894
Mph1103I	ATGCAT	5 cut(s)	26, 2998, 4981, 6000, 6931
MroI	TCCGGA	5 cut(s)	64, 466, 3357, 5920, 6864
MroXI	GAANNNNTTC	7 cut(s)	462, 1180, 4137, 5280, 5661, 6497, 6726
MscI	TGGCCA	2 cut(s)	710, 894
MslI	CAYNNNNRTG	8 cut(s)	1470, 1974, 2559, 2609, 3348, 4046, 5289, 6924
Msp20I	TGGCCA	2 cut(s)	710, 894
MspA1I	CMGCKG	1 cut(s)	3037
MunI	CAATTG	3 cut(s)	3236, 6367, 6758
Mva1269I	GAATGC	3 cut(s)	6242, 6273, 6646
MvnI	CGCG	4 cut(s)	1494, 1515, 3037, 6536
NciI	CCSGG	5 cut(s)	1309, 1712, 2364, 4427, 6209
NcoI	CCATGG	1 cut(s)	3626
NdeI	CATATG	8 cut(s)	1360, 1402, 1423, 1444, 1486, 1507, 1528, 1549
NmeAIII	GCCGAG	4 cut(s)	349, 4688, 5757, 6871
NruI	TCGCGA	2 cut(s)	1494, 1515
NsiI	ATGCAT	5 cut(s)	26, 2998, 4981, 6000, 6931
NspI	RCATGY	8 cut(s)	24, 28, 1469, 2045, 3000, 3549, 3673, 3887
NspV	TTCGAA	3 cut(s)	5695, 6438, 7199
PaeI	GCATGC	2 cut(s)	24, 28
PagI	TCATGA	6 cut(s)	1429, 3559, 4581, 5182, 5284, 6793
PceI	AGGCCT	1 cut(s)	3314
PciI	ACATGT	5 cut(s)	1465, 2041, 3545, 3669, 3883
PciSI	GCTCTTC	2 cut(s)	1986, 6346
PctI	GAATGC	3 cut(s)	6242, 6273, 6646
PdmI	GAANNNNTTC	7 cut(s)	462, 1180, 4137, 5280, 5661, 6497, 6726
PmaCI	CACGTG	3 cut(s)	1298, 1703, 4250
PmlI	CACGTG	3 cut(s)	1298, 1703, 4250
Ppu21I	YACGTR	5 cut(s)	1298, 1451, 1703, 4250, 6104
PpuMI	RGGWCCY	4 cut(s)	1305, 1374, 3287, 4022
PscI	ACATGT	5 cut(s)	1465, 2041, 3545, 3669, 3883
PshAI	GACNNNNGTC	1 cut(s)	1332
PshBI	ATTAAT	5 cut(s)	1881, 3633, 4083, 4749, 4770
PsiI	TTATAA	3 cut(s)	2951, 3582, 4539
Psp124BI	GAGCTC	3 cut(s)	2158, 5230, 6620
Psp1406I	AACGTT	1 cut(s)	4444
Psp5II	RGGWCCY	4 cut(s)	1305, 1374, 3287, 4022
PspCI	CACGTG	3 cut(s)	1298, 1703, 4250
PspEI	GGTNACC	3 cut(s)	1695, 1706, 3152
PspFI	CCCAGC	3 cut(s)	250, 3272, 3864
PspOMI	GGGCCC	1 cut(s)	7009
PspPPI	RGGWCCY	4 cut(s)	1305, 1374, 3287, 4022
PstI	CTGCAG	2 cut(s)	3604, 5898
PstNI	CAGNNNCTG	4 cut(s)	3520, 4222, 5201, 6326
RruI	TCGCGA	2 cut(s)	1494, 1515
RseI	CAYNNNNRTG	8 cut(s)	1470, 1974, 2559, 2609, 3348, 4046, 5289, 6924
SacI	GAGCTC	3 cut(s)	2158, 5230, 6620
SacII	CCGCGG	1 cut(s)	3038
SapI	GCTCTTC	2 cut(s)	1986, 6346
ScaI	AGTACT	3 cut(s)	874, 1208, 5101
SduI	GDGCHC	7 cut(s)	2158, 2337, 2431, 5230, 5778, 6620, 7013
SfcI	CTRYAG	5 cut(s)	721, 3308, 3600, 5488, 5894
Sfr303I	CCGCGG	1 cut(s)	3038
SfuI	TTCGAA	3 cut(s)	5695, 6438, 7199
SgrBI	CCGCGG	1 cut(s)	3038
SmiMI	CAYNNNNRTG	8 cut(s)	1470, 1974, 2559, 2609, 3348, 4046, 5289, 6924
SmlI	CTYRAG	5 cut(s)	2547, 3445, 3838, 6515, 6773
SmoI	CTYRAG	5 cut(s)	2547, 3445, 3838, 6515, 6773
SnaBI	TACGTA	1 cut(s)	6104
SpeI	ACTAGT	1 cut(s)	2278
SphI	GCATGC	2 cut(s)	24, 28
SseBI	AGGCCT	1 cut(s)	3314
SstI	GAGCTC	3 cut(s)	2158, 5230, 6620
StuI	AGGCCT	1 cut(s)	3314
TaqII	GACCGA	2 cut(s)	3072, 3171
TatI	WGTACW	8 cut(s)	872, 957, 969, 1206, 3672, 4380, 5099, 6878
TauI	GCSGC	3 cut(s)	1746, 3037, 5647
TspGWI	ACGGA	4 cut(s)	1612, 1841, 6167, 6610
VspI	ATTAAT	5 cut(s)	1881, 3633, 4083, 4749, 4770
XagI	CCTNNNNNAGG	1 cut(s)	5588
XbaI	TCTAGA	4 cut(s)	1366, 2395, 2759, 5458
XceI	RCATGY	8 cut(s)	24, 28, 1469, 2045, 3000, 3549, 3673, 3887
XcmI	CCANNNNNNNNNTGG	2 cut(s)	751, 5245
XmaJI	CCTAGG	1 cut(s)	1649
XmiI	GTMKAC	2 cut(s)	945, 4005
XmnI	GAANNNNTTC	7 cut(s)	462, 1180, 4137, 5280, 5661, 6497, 6726
ZraI	GACGTC	1 cut(s)	4914
ZrmI	AGTACT	3 cut(s)	874, 1208, 5101
Zsp2I	ATGCAT	5 cut(s)	26, 2998, 4981, 6000, 6931

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.